guanosine-monophosphate and benzothiazole

guanosine-monophosphate has been researched along with benzothiazole* in 1 studies

Other Studies

1 other study(ies) available for guanosine-monophosphate and benzothiazole

Article	Year
AMP/GMP Analogs as Affinity ESIPT Probes for Highly Selective Sensing of Alkaline Phosphatase Activity in Living Systems. Chemistry, an Asian journal, 2015, Volume: 10, Issue:11 Current probes for alkaline phosphatase (ALP) detection had been developed mainly by adding a phosphate group to a dye, which would lead to indistinct performance when implemented in a living system as several phosphatases exist together. In this study, the nucleotides adenosine monophosphate (AMP) and guanosine monophosphate (GMP) were introduced into 2'-(2'-hydroxyphenyl)-benzothiazole-based probes, and highly fluorescent turn-on probes with good selectivity towards ALP over several phosphatases, as well as high affinity and low toxicity were obtained. In the presence of L-phenylalanine, an ALP inhibitor, a strong decrease in fluorescence recovery was observed. These probes allowed for real-time imaging of endogenous ALP activity in living cells as well as in a zebrafish model. Topics: Adenosine Monophosphate; Alkaline Phosphatase; Animals; Benzothiazoles; Fluorescent Dyes; Guanosine Monophosphate; HeLa Cells; Humans; Microscopy, Confocal; Zebrafish	2015

Article

Year

AMP/GMP Analogs as Affinity ESIPT Probes for Highly Selective Sensing of Alkaline Phosphatase Activity in Living Systems.

Chemistry, an Asian journal, 2015, Volume: 10, Issue:11

Current probes for alkaline phosphatase (ALP) detection had been developed mainly by adding a phosphate group to a dye, which would lead to indistinct performance when implemented in a living system as several phosphatases exist together. In this study, the nucleotides adenosine monophosphate (AMP) and guanosine monophosphate (GMP) were introduced into 2'-(2'-hydroxyphenyl)-benzothiazole-based probes, and highly fluorescent turn-on probes with good selectivity towards ALP over several phosphatases, as well as high affinity and low toxicity were obtained. In the presence of L-phenylalanine, an ALP inhibitor, a strong decrease in fluorescence recovery was observed. These probes allowed for real-time imaging of endogenous ALP activity in living cells as well as in a zebrafish model.

Topics: Adenosine Monophosphate; Alkaline Phosphatase; Animals; Benzothiazoles; Fluorescent Dyes; Guanosine Monophosphate; HeLa Cells; Humans; Microscopy, Confocal; Zebrafish

2015