guanosine-diphosphate and retinol-phosphate

Topics: Animals; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cricetinae; Diterpenes; Dolichol Monophosphate Mannose; Guanosine Diphosphate; Intracellular Membranes; Liver; Mannose; Mannosyltransferases; Mesocricetus; Polyisoprenyl Phosphate Monosaccharides; Polyisoprenyl Phosphate Sugars; Rats; Vitamin A

Rat liver microsomes catalyze the transfer of mannose from GDPmannose to both retinyl phosphate and dolichyl phosphate to form mannosylphosphorylretinol, mannosylphosphoryldolichol and GDP. The two reactions differ in term of reversibility. In fact, a 200-fold isotopic dilution of GDP[14C]mannose by unlabeled GDPmannose causes mannosylphosphoryldolichol labeling to disappear almost completely, while mannosylphosphorylretinol labeling remains at the same level. The same observation can be made if the mannose donor is removed by centrifugation and replaced by excess GDP; again mannosylphosphorylretinol is stable, but mannosylphosphoryldolichol drops down to one-third of its initial level, as expected for, respectively, a non-reversible and a reversible reaction. Placed in an aqueous medium, mannosylphosphorylretinol releases mannose 1-phosphate (beta configuration) whereas it is quite stable when kept in a membranous environment. These results strongly suggest that mannosylphosphorylretinol as soon as it is formed is segregated in such a way that it is no longer available to the back-reaction; the functional consequence of this segregation would be the possibility for mannosylphosphorylretinol to mannosylate some non-polar regions of certain protein chains.

Topics: Animals; Cell Compartmentation; Diterpenes; Dolichol Monophosphate Mannose; Dolichol Phosphates; Glycosides; Guanosine Diphosphate; Hexosyltransferases; Male; Mannose; Mannosides; Mannosyltransferases; Microsomes, Liver; Polyisoprenyl Phosphate Sugars; Polyisoprenyl Phosphates; Rats; Vitamin A