guanosine-diphosphate and inositol-1-4-5-trisphosphate-1-(2-nitrophenyl)ethyl-ester

guanosine-diphosphate has been researched along with inositol-1-4-5-trisphosphate-1-(2-nitrophenyl)ethyl-ester* in 1 studies

Other Studies

1 other study(ies) available for guanosine-diphosphate and inositol-1-4-5-trisphosphate-1-(2-nitrophenyl)ethyl-ester

Article	Year
Rapid coupling of calcium release to depolarization in Limulus polyphemus ventral photoreceptors as revealed by microphotolysis and confocal microscopy. The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997, Mar-01, Volume: 17, Issue:5 Microphotolysis and confocal microscopy were used to investigate the timing of calcium release and of the electrical response in Limulus polyphemus ventral photoreceptors. The fluorescent dyes Fluo-3 and Calcium Green-5N were used to monitor local Ca2+ elevations. Photolysis of caged inositol trisphosphate (InsP3) close to the plasma membrane of the light-sensitive rhabdomeral (R-) lobe resulted in Ca2+ elevation within 10-20 msec, 20-45 msec before the physiological response to light normally would be detected. Inward ionic current flow and depolarization followed InsP3-induced calcium release within 2.5 +/- 3.3 msec. Voltage-clamping the cells and removal of extracellular Ca2+ did not affect the timing of the Ca2+ elevation that followed the photolysis of caged InsP3 or its relationship to the electrical response. In contrast to the physiological response to light, which only released calcium within the R-lobe, photolysis of InsP3 elevated Cai in both lobes, although with much greater effect in the R-lobe, as compared with the bulk of the A-lobe, suggesting the presence of InsP3-sensitive calcium stores in both lobes. Photolysis of caged calcium [o-nitrophenyl EGTA (NPE)] at the edge of the R-lobe activated an inward ionic current within 1.8 +/- 0.7 msec. This NPE-induced current reversed at a membrane potential of 10 +/- 6 mV in the range typical of that of the light-activated current under physiological conditions. Calcium release, therefore, activates an inward current rapidly enough to contribute to the electrical response to light. Topics: Action Potentials; Adenosine Triphosphate; Aniline Compounds; Animals; Calcium; Drosophila melanogaster; Egtazic Acid; Fluorescent Dyes; Guanosine Diphosphate; Horseshoe Crabs; Inositol 1,4,5-Trisphosphate; Microchemistry; Microscopy, Confocal; Optic Nerve; Organic Chemicals; Patch-Clamp Techniques; Photolysis; Photoreceptor Cells, Invertebrate; Second Messenger Systems; Species Specificity; Thionucleotides; Xanthenes	1997

Article

Year

Rapid coupling of calcium release to depolarization in Limulus polyphemus ventral photoreceptors as revealed by microphotolysis and confocal microscopy.

The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997, Mar-01, Volume: 17, Issue:5

Microphotolysis and confocal microscopy were used to investigate the timing of calcium release and of the electrical response in Limulus polyphemus ventral photoreceptors. The fluorescent dyes Fluo-3 and Calcium Green-5N were used to monitor local Ca2+ elevations. Photolysis of caged inositol trisphosphate (InsP3) close to the plasma membrane of the light-sensitive rhabdomeral (R-) lobe resulted in Ca2+ elevation within 10-20 msec, 20-45 msec before the physiological response to light normally would be detected. Inward ionic current flow and depolarization followed InsP3-induced calcium release within 2.5 +/- 3.3 msec. Voltage-clamping the cells and removal of extracellular Ca2+ did not affect the timing of the Ca2+ elevation that followed the photolysis of caged InsP3 or its relationship to the electrical response. In contrast to the physiological response to light, which only released calcium within the R-lobe, photolysis of InsP3 elevated Cai in both lobes, although with much greater effect in the R-lobe, as compared with the bulk of the A-lobe, suggesting the presence of InsP3-sensitive calcium stores in both lobes. Photolysis of caged calcium [o-nitrophenyl EGTA (NPE)] at the edge of the R-lobe activated an inward ionic current within 1.8 +/- 0.7 msec. This NPE-induced current reversed at a membrane potential of 10 +/- 6 mV in the range typical of that of the light-activated current under physiological conditions. Calcium release, therefore, activates an inward current rapidly enough to contribute to the electrical response to light.

Topics: Action Potentials; Adenosine Triphosphate; Aniline Compounds; Animals; Calcium; Drosophila melanogaster; Egtazic Acid; Fluorescent Dyes; Guanosine Diphosphate; Horseshoe Crabs; Inositol 1,4,5-Trisphosphate; Microchemistry; Microscopy, Confocal; Optic Nerve; Organic Chemicals; Patch-Clamp Techniques; Photolysis; Photoreceptor Cells, Invertebrate; Second Messenger Systems; Species Specificity; Thionucleotides; Xanthenes

1997