guanosine-diphosphate and 2-5-di-tert-butylhydroquinone

The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine 5'-[gamma thio]triphosphate (GTP gamma S) (introduced into the cytoplasmic space by microinjection), thapsigargin and 2,5-di-tert- butylhydroquinone (DBHQ) to stimulate Ca2+ inflow. Photolysis of 'caged' GPIP2 or 'caged' GTP gamma S stimulated Ca2+ inflow. The abilities of GPIP2, thapsigargin and DBHQ to stimulate Ca2+ inflow were inhibited by the pre-treatment of hepatocytes with pertussis toxin in vivo for 36 h. Thapsigargin-stimulated Ca2+ inflow was also inhibited by guanosine 5'-[beta-thio]diphosphate (GDP beta S) (introduced by microinjection). It is concluded that, in hepatocytes, store-operated Ca2+ inflow induced by the actions of either inositol 1,4,5-trisphosphate, thapsigargin or DBHQ requires a pertussis toxin-sensitive trimeric G-protein.

Topics: Aniline Compounds; Animals; Calcium; Fluorescent Dyes; Fura-2; GTP-Binding Proteins; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Diphosphate; Hydroquinones; Inositol Phosphates; Liver; Macromolecular Substances; Pertussis Toxin; Phosphatidylinositol Phosphates; Photolysis; Rats; Terpenes; Thapsigargin; Thionucleotides; Virulence Factors, Bordetella; Xanthenes