guanidine has been researched along with urea in 682 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 107 (15.69) | 18.7374 |
| 1990's | 181 (26.54) | 18.2507 |
| 2000's | 232 (34.02) | 29.6817 |
| 2010's | 142 (20.82) | 24.3611 |
| 2020's | 20 (2.93) | 2.80 |
| Authors | Studies |
|---|---|
| Cardamone, M; Puri, NK | 1 |
| Jaenicke, R; Kern, G; Schmid, FX; Schülke, N | 1 |
| Neet, KE; Timm, DE | 1 |
| Darszon, A; Fernández-Velasco, DA; Garza-Ramos, G; Gómez-Puyou, A; Ramírez, L; Shoshani, L; Tuena de Gómez-Puyou, M | 2 |
| Koshy, TI; Luntz, TL; Margoliash, E; Schejter, A | 1 |
| Lukaszuk, C; Rółkowski, R; Skrzydlewski, Z; Worowski, K | 1 |
| Gierasch, LM; Landry, SJ; Srere, PA; Zhi, W | 1 |
| Fountoulakis, M | 1 |
| Amir, D; Haas, E; Krausz, S | 1 |
| Fang, D; Gu, W; Hachiya, K; Hamada, S; Sasa, K; Shigemura, A; Takeda, K | 1 |
| Dyer, JM; Murai, N; Nelson, JW | 1 |
| Timasheff, SN | 1 |
| Martorana, GE; Meucci, E; Miggiano, GA; Mordente, A; Santini, SA | 1 |
| Goñi, FM; Rial, E; Viguera, AR | 1 |
| Breslow, R; Halfon, S | 1 |
| Bertolini, J; Brandon, MR; Cardamone, M; Crivelli, E; Fiddes, R; Ninham, B; Puri, NK | 1 |
| Asboth, B; Hartley, BS; Rangarajan, M | 1 |
| Agarwal, SK; Ahmad, S; Khan, MY | 1 |
| Chaffotte, AF; Goldberg, ME; Guillou, Y | 1 |
| Busath, D; Hemsley, G | 1 |
| Batra, PP | 1 |
| Bayer, EA; Horowitz, PM; Kurzban, GP; Wilchek, M | 1 |
| Asel, ED; Bird, RE; Dodd, SW; Hardman, KD; Johnson, S; Pantoliano, MW; Wood, JF | 1 |
| Bujalowski, W; Lohman, TM | 1 |
| Agarwal, SK; Das, BK; Khan, MY | 1 |
| Damodaran, S; Deshpande, SS | 1 |
| Hahn, U; Kiefhaber, T; Quaas, R; Schmid, FX | 1 |
| Cousins, CJ; Hamilton, MG; Herskovits, TT; Wall, JS | 1 |
| Breslow, R; Guo, T | 1 |
| Kieft, TL; Ruscetti, T | 1 |
| Coggins, JR; Kleanthous, C | 1 |
| Choma, CT; Kaplan, H | 1 |
| Cerletti, P; Guerrieri, N | 1 |
| De Deyn, PP; Hogaerth, R; Lowenthal, A; Marescau, B; Possemiers, I; Swartz, RD | 1 |
| Batra, PP; Uetrecht, D | 1 |
| Biberfeld, P; Collins, VP; Costa, PP; Jacobsson, B | 1 |
| Ayrault-Jarrier, M; Bekaert, ED; Betourne, C; Petit, E; Polonovski, J; Robin, H | 1 |
| Bras, GL; Deville-Bonne, D; Garel, JR; Teschner, W | 1 |
| Gerring, SL; Meeker, AK; Shortle, D | 1 |
| Ascoli, F; Giartosio, A; Santucci, R | 1 |
| Autuori, F; Ceru, MP; Fesus, L; Piacentini, M; Tarcsa, E; Thomazy, V | 1 |
| Batra, PP; Sasa, K; Takeda, K; Ueki, T | 1 |
| Bucci, E; Fronticelli, C; Malak, H | 1 |
| Berger, JW; Vanderkooi, JM | 1 |
| Creighton, DJ; Pourmotabbed, T; Shih, MJ | 1 |
| Moriyama, Y; Takeda, K | 1 |
| Büscher, KH; Karch, H; Kroll, HP; Leying, H; Opferkuch, W | 1 |
| Gonnelli, M; Strambini, GB | 3 |
| Bryant, C; Stellwagen, E; Strottmann, JM | 1 |
| Fares, F; Gavish, M | 1 |
| Caldwell, SE; Lyles, DS | 1 |
| Deutsch, HF; Han, KK; Xu, LX; Zhang, A; Zhu, DX; Zhu, NC | 1 |
| Arends, J; Meiring, HD; ten Bosch, JJ; vd Linden, AH | 1 |
| Hamilton, MG; Herskovits, TT | 3 |
| Batra, PP; Nagao, M; Sasa, K; Takeda, K | 1 |
| Bolen, DW; Santoro, MM | 1 |
| Kim, CS; Prakash, V; Rao, AG | 1 |
| Beckman, SB; Duffy, TH; Huennekens, FM; Peterson, SM; Vitols, KS | 1 |
| Liang, JN; Pelletier, MR | 1 |
| White, TK; Wilson, JE | 1 |
| Mitchell, WR | 1 |
| Meeker, AK; Shortle, D | 1 |
| Arakawa, T; Boone, T; Davis, JM; Kenney, WC | 1 |
| Snyder, GH | 1 |
| Masucci, MT; Petrillo, A; Sica, V | 1 |
| Lutz, WK; Schlatter, C; Shephard, SE | 1 |
| Bootello, A; Ferreira, A; Gonzalez-Porqué, P; Revilla, Y; Villar, ML | 1 |
| Pace, CN | 1 |
| Barnes, C; Evans, JA; Lewis, TJ | 1 |
| Carberry, SE; Herskovits, TT; Villanueva, GB | 1 |
| Bascom, CC; Johnson, TC; Khurana, VK; Sharifi, BG | 1 |
| Herskovits, TT; Mazzella, LJ; Villanueva, GB | 1 |
| Konishi, Y; Mui, PW; Scheraga, HA | 1 |
| Arakawa, T; Timasheff, SN | 1 |
| Anderton, B | 1 |
| Munro, MH; Rinehart, KL; Stroshane, RM | 1 |
| Deal, CD; Kaplan, S | 1 |
| Lovell, SJ; Piper, JM | 1 |
| Little, C; Olsen, RL | 1 |
| Lennon, VA; Momoi, MY | 1 |
| Hryniewicz, W; Jeljaszewicz, J; Tyski, S | 1 |
| Price, NC; Stevens, E | 1 |
| Herskovits, TT; Russell, MW | 1 |
| Nandi, PK; Robinson, DR | 1 |
| Bowden, GT; Cress, AE | 1 |
| Gold, W; Lowary, RL; Moreno, OM; Saffir, AJ; Yankell, SL | 1 |
| Coulet, PR; Gautheron, DC | 1 |
| Asher, CJ; Blakeley, RL; Dixon, NE; Gazzola, C; Lee, DS; Zerner, B | 1 |
| Hirano, K; Samejima, T; Shiroya, Y; Takeda, A | 1 |
| Ahmad, F | 1 |
| Bindels, JG; Siezen, RJ | 1 |
| Wagner, PD | 1 |
| Kreutzberger, A; Schimmelpfennig, H | 1 |
| Allegra, P; Altieri, F; Caiafa, P; Lonigro, R; Scarpati-Cioffari, MR; Turano, C | 1 |
| Keene, JL; Ross, P; Ruh, TS; Wood, DM | 1 |
| Badcoe, IG; Clarke, AR; Edwards, HG; Jackson, GS; Mehta, V; Taylor, LS; Williams, AC; York, P | 1 |
| de Windt, FE; van der Drift, C; Xu, Z | 1 |
| Fesik, SW; Logan, TM; Thériault, Y | 1 |
| Creighton, TE; Kemmink, J | 1 |
| Gindzieński, A; Paszkiewicz-Gadek, A; Porowska, H | 1 |
| Clinkenbeard, KD; Clinkenbeard, PA; Waurzyniak, BJ | 1 |
| Edwards, MD; Hamilton, MG; Herskovits, TT | 1 |
| Hodges, RS; Kay, CM; Kohn, WD; Monera, OD | 1 |
| Chang, JY | 1 |
| Goto, Y; Kataoka, M; Nishii, I | 1 |
| Fermandjian, S; Frère, V; Monnot, M; Sourgen, F; Troalen, F | 1 |
| Shirley, BA | 1 |
| Furter, R; Gross, M; Lustig, A; Wallimann, T | 1 |
| Rao, KN | 2 |
| Alagiri, S; Singh, TP | 1 |
| Hodges, RS; Kay, CM; Monera, OD | 1 |
| Ramasarma, PR; Rao, AG; Rao, DR | 1 |
| Shen, S; Strobel, HW; Yu, XC | 1 |
| Bishop, PD; Ingham, KC; Kurochkin, IV; Medved, LV; Procyk, R; Teller, DC; Yee, VC | 1 |
| McClelland, DA; Price, NC; Stevens, L | 1 |
| Fukumura, M; Ohmiya, K; Sakka, K; Tanaka, A | 1 |
| Moosavi-Movahedi, AA; Nazari, K | 1 |
| De Deyn, PP; Marescau, B; Qureshi, IA; Robitaille, P; Vanasse, M | 1 |
| DeKoster, GT; Robertson, AD | 2 |
| Mironov, SL | 1 |
| Desmadril, M; Garcia, P; Minard, P; Yon, JM | 1 |
| Gibbons, DL; Horowitz, PM; Hua, S | 1 |
| Lorimer, GH; Todd, MJ | 1 |
| Grötzinger, J; Kula, MR; Pohl, M; Wollmer, A | 1 |
| Marino, G; Pucci, P; Ruoppolo, M; Torella, C | 1 |
| Mücke, M; Schmid, FX | 1 |
| Hamada, S; Moriyama, Y; Takeda, K; Yamaguchi, K | 1 |
| Builder, SE; Giltinan, DM; Hart, RA; Lester, PM; Ogez, JR; Reifsnyder, H | 1 |
| Faguy, DM; Jarrell, KF; Koval, SF | 1 |
| Feller, G; Gerday, C; Haser, R; Payan, F; Qian, M; Theys, F | 1 |
| Friedman, M; Gorinstein, S; Greene, LL; Katzhendler, J; Minsky, A; Zemser, M | 1 |
| Calaycay, J; Chabin, R; Covey, TR; Green, BG; Knight, WB; Lee, TD; Sakuma, T; Shively, JE; Shushan, B; Swiderek, KM | 1 |
| Butterfield, DA; Lee, J | 1 |
| Hedman, K; Polanec, J; Rousseau, S; Seppälä, I | 1 |
| Bessalle, R; D'Andrea, A; Fasman, G | 1 |
| Jaenicke, R; Lehle, K; Wrba, A | 1 |
| Ahmad, F; Haque, SE; Taneja, S; Yadav, S | 1 |
| Fountoulakis, M; Juranville, JF | 1 |
| Uversky, VN | 2 |
| Böhm, G; Jaenicke, R; Rudolph, R; Schumacher, G; Schumann, J | 1 |
| Robertson, AD; Swint, L | 1 |
| Mozhaev, VV; Panova, AA | 1 |
| Baracca, A; Parenti Castelli, G; Solaini, G; Strambini, GB | 1 |
| Coffino, P; Tsirka, SE; Turck, CW | 1 |
| Messinger, J; Renger, G; Schröder, WP | 1 |
| Mayr, LM; Schmid, FX | 1 |
| Pinto, A; Sorrentino, L; Sorrentino, R | 1 |
| Rossi, SG; Rotundo, RL | 1 |
| Booy, FP; Brown, JC; Newcomb, WW; Steven, AC; Trus, BL; Wall, JS | 1 |
| Khurgin, Yu; Maksareva, E | 1 |
| Gilbert, HF; McKeone, BJ; Morjana, NA | 1 |
| Atkinson, T; Badcoe, IG; Burston, SG; Clarke, AR; Holbrook, JJ; Jackson, GS; Smith, CJ; Staniforth, RA | 1 |
| Clarke, J; Fersht, AR | 1 |
| Tsou, CL | 1 |
| Campbell-Burk, SL; Carpenter, JW | 1 |
| Kanaya, S; Ogasahara, K; Oobatake, M; Yamasaki, K; Yutani, K | 1 |
| Clottes, E; Couthon, F; Ebel, C; Vial, C | 1 |
| Bolen, DW; Huang, Y | 1 |
| Ptitsyn, OB | 1 |
| Fee, L; Gray, T; Grimsley, G; Pace, CN; Vajdos, F | 1 |
| Midura, RJ; Yanagishita, M | 1 |
| Manning, JM; Peisach, D; Petsko, G; Pospischil, MA; Ringe, D; Soda, K; Van Ophem, PW | 1 |
| Beck, K; Nomizu, M; Otaka, A; Roller, PP; Utani, A; Yamada, Y | 1 |
| Chottard, G; Kuchumov, AR; Martin, PD; Sharma, PK; Vinogradov, SN; Wall, JS | 1 |
| Macek, P; Malavasic, M; Poklar, N; Vesnaver, G | 1 |
| Rao, AG; Srinivasulu, S | 1 |
| Blackburn, MN; Peterson, CB; Zhuang, P | 1 |
| Fan, YX; Ju, M; Tsou, CL; Zhou, JM | 1 |
| Gassner, NC; Schellman, JA | 1 |
| Agarwalla, S; Balaram, P; Gokhale, RS; Santi, DV | 1 |
| Scholtz, JM; Smith, JS | 1 |
| Elhammer, A; Nagaraj, RH; Perry, G; Richey, PL; Siedlak, SL; Smith, MA | 1 |
| Lapanje, S; Poklar, N; Vesnaver, G | 1 |
| Iuchi, I; Murata, K; Sugiyama, H; Yamagami, K | 1 |
| Gopalakrishna, KN; Prakash, V; Rajeshwara, AN | 1 |
| Ahmad, F; Gupta, R; Yadav, S | 1 |
| Colombini, M; Xu, X | 1 |
| Kaur, H; Salahuddin, A | 1 |
| Bannister, JV; Bocchini, V; Dello Russo, A; Masullo, M; Raimo, G | 1 |
| Camacho, CJ; Thirumalai, D | 1 |
| Mas, MT; Szpikowska, BK | 1 |
| Bowler, BE; Dong, A; Garbin, K; Godbole, S | 1 |
| Ahmad, F; Moosavi-Movahedi, AA; Shareghi, B | 1 |
| Bryson, JW; DeGrado, WF; Lombardi, A | 1 |
| Ptitsyn, OB; Uversky, VN | 1 |
| Amicosante, G; Frère, JM; Galleni, M; Gicquel, B; Quinting, B; Timm, J | 1 |
| Gauthier, M; Patston, PA | 1 |
| Glockshuber, R; Jaenicke, R; Mayr, EM | 1 |
| Jaenicke, R; Palme, S; Slingsby, C | 1 |
| Chacón, P; De Meester, I; Devreese, B; Díaz Pereira, JF; Heremans, K; Lambeir, AM; Scharpé, S; Van Beeumen, J; Vermeulen, G | 1 |
| Banerjee, S; Dunbar, J; Farber, GK; Luo, J; Yennawar, HP | 1 |
| Amemiya, Y; Fan, YX; Kihara, H; Kimura, K; Zhou, JM | 1 |
| Rykke, M; Rølla, G; Smistad, G; Young, A | 1 |
| Grimsley, JK; Pace, CN; Scholtz, JM; Wild, JR | 1 |
| Carlson, KE; Choi, I; Gee, A; Katzenellenbogen, BS; Katzenellenbogen, JA | 1 |
| Li, S; Yang, HP; Zhou, HM | 1 |
| Lopez, MM; Makhatadze, GI; Richardson, JM; Thomas, ST | 1 |
| Allocatelli, CT; Arese, M; Bigotti, MG; Brunori, M; Cutruzzolà, F; Staniforth, RA | 1 |
| Klimov, DK; Thirumalai, D | 1 |
| Britton, KL; Chiaraluce, R; Consalvi, V; Engel, PC; Giangiacomo, L; Millevoi, S; Pasquo, A; Rice, DW; Stillman, TJ | 1 |
| Denson, AM; Doyle, RJ | 1 |
| Budisa, N; Golbik, R; Huber, R; Minks, C; Moroder, L; Weyher, E | 1 |
| Teng, CC; Yang, YW | 1 |
| Burton, RE; Ghaemmaghami, S; Oas, TG; Richardson, JS; Word, JM | 1 |
| Das, M; Dasgupta, D | 1 |
| Baldwin, MA; Bose, HS; Miller, WL | 1 |
| Fürlinger, M; Haltrich, D; Kulbe, KD; Nidetzky, B; Satory, M | 1 |
| Horowitz, PM; Masters, BS; McMillan, K; Narayanasami, R; Nishimura, JS; Robida, AM; Roman, LJ; Shea, TM | 1 |
| Choe, SE; Matsudaira, PT; Osterhout, J; Shakhnovich, EI; Wagner, G | 1 |
| Das, BK; Liang, JJ | 1 |
| Balaram, H; Balaram, P; Gokhale, RS; Ray, SS | 1 |
| Carew, EL; Doss-Pepe, EW; Koretz, JF | 1 |
| Ahmad, F; Gupta, R | 1 |
| Brown, BM; Sauer, RT | 1 |
| Bonete, MJ; Cremades, R; Ferrer, J; Pire, C | 1 |
| Francky, A; Janjić, V; Mozetic-Francky, B; Zerovnik, E | 1 |
| Loewen, PC; O'Neil, JO; Switala, J | 1 |
| Raleigh, DP; Spector, S; Young, P | 1 |
| Arai, M; Fujiwara, K; Ikeguchi, M; Kuwajima, K; Shimizu, A; Sugai, S | 1 |
| Kelly, JW; Koepf, EK; Petrassi, HM; Sudol, M | 1 |
| Fu, X; Inouye, M; Shinde, U | 1 |
| De Bernardez Clark, E; Schauer, N; Tran-Moseman, A | 1 |
| DiNardo, AA; Merrill, AR; Steer, BA | 1 |
| Fan, YX; McPhie, P; Miles, EW | 1 |
| Plückthun, A; Wörn, A | 1 |
| Martins, NF; Santoro, MM | 1 |
| Chang, JY; Lai, PH; Märki, W | 1 |
| Borchard, W; Flemming, HC; Kirschner, C; Maibaum, R; Mayer, C; Moritz, R; Wingender, J | 1 |
| Grier, DG; Preuss, D; Zinkl, GM; Zwiebel, BI | 1 |
| Chen, GQ; Gouaux, E | 1 |
| Rutkowska, SM; Skowron, PM | 1 |
| Behere, DV; Deva, MS | 1 |
| Hodges, RS; Lavigne, P; Mant, C; Tripet, B; Wagschal, K | 1 |
| Farruggia, B; Picó, GA | 1 |
| Chen, SG; Gambetti, P; Morillas, M; Surewicz, WK; Swietnicki, W | 1 |
| Chang, GG; Chang, HC; Lin, TL | 1 |
| Chakraborty, S; Peng, Z | 1 |
| Chen, J; Smith, DL | 1 |
| Briganti, F; Clare, BW; Scozzafava, A; Supuran, CT | 1 |
| Argaraña, CE; Fidelio, GD; González, M | 1 |
| Bachinger, HP; Mechling, DE | 1 |
| Ballatore, A; Chang, J | 1 |
| Campoy, FJ; Fini, C; Flores-Flores, C; Martínez-Martínez, A; Muñoz-Delgado, E; Rodríguez-López, JN; Vidal, CJ | 1 |
| Christova, P; Cox, JA; Craescu, CT | 1 |
| Eastman, P; Fink, AL; Nishimura, C; Riley, R | 1 |
| Backmann, J; Dao-Thi, MH; Messens, J; Wyns, L | 1 |
| Cao, ZF; Wang, GF; Zhao, YF; Zhou, HM | 1 |
| Hsieh, H; Kumar, TK; Samuel, D; Srimathi, T; Yu, C | 1 |
| Hirose, M; Muralidhara, BK | 1 |
| Stephens, RE | 1 |
| Knapp, M; Lund-Katz, S; Morrow, JA; Phillips, MC; Rupp, B; Segall, ML; Weisgraber, KH | 1 |
| Capp, MW; Courtenay, ES; Record, MT; Saecker, RM | 1 |
| Armstrong, RN; Dirr, HW; Hornby, JA; Kaplan, W; Luo, JK; Stevens, JM; Wallace, LA | 1 |
| Cigić, B; Dahl, SW; Pain, RH | 1 |
| Goto, J; Hazeki, N; Kanazawa, I; Tukamoto, T | 1 |
| Cape, SP; Carpenter, JF; Chi, E; Kim, YS; Manning, MC; Raffen, R; Randolph, TW; Solomon, A; Stevens, FJ; Wilkins-Stevens, P | 1 |
| García-Moreno E, B; Whitten, ST | 1 |
| Aguzzi, A; Fischer, MB; Parizek, P; Roeckl, C; Schwarz, HP | 1 |
| Chang, JY; Li, L | 2 |
| Besman, MJ; Edwards, KL; Grillo, AO; Hu, L; Kashi, RS; Middaugh, CR; Shipley, KM | 1 |
| Guidry, JJ; Landry, SJ; Moczygemba, CK; Steede, NK; Wittung-Stafshede, P | 1 |
| Aoyagi, K; Cohen, BD; Hirayama, A; Iida, S; Koyama, A; Nagase, S; Narita, M; Ohba, S; Shahrzad, S; Takemura, K; Tomida, C | 1 |
| Azumaya, I | 1 |
| Domínguez-Ramírez, L; Gómez-Puyou, A; Reyes-Vivas, H; Tuena de Gómez-Puyou, M | 1 |
| Guidry, J; Pozdnyakova, I; Wittung-Stafshede, P | 1 |
| Abrashev, R; Dolashka-Angelova, P; Nicolov, P; Schütz, J; Voelter, W | 1 |
| Amici, M; Angeletti, M; Eleuteri, AM; Fioretti, E; Lupidi, G; Marchini, C; Pucciarelli, S | 1 |
| Xie, ZQ; Xu, GJ; Yuan, C; Zhang, FW | 1 |
| Deshpande, RA; Khan, MI; Kumar, AR; Shankar, V | 1 |
| Buche, A; Ellis, G; Ramirez, JM | 1 |
| Bigotti, MG; Brunori, M; Cutruzzolà, F; Gianni, S; Travaglini-Allocatelli, C | 2 |
| Beck, PJ; Chang, JY; Lu, BY | 1 |
| Stauffer, CE; Sullivan, JF | 1 |
| Perrin, CL; Young, DB | 1 |
| Lonzer, WL; Nagy, JK; Sanders, CR | 1 |
| Brunori, M; Gianni, S; Travaglini-Allocatelli, C | 1 |
| Hayer-Hartl, M; Weber, F | 1 |
| Ejima, D; Harada, T; Kato, K; Kurimoto, E; Moriyama, Y; Nohara, D; Sakai, T | 1 |
| Hammarström, P; Jiang, X; Kelly, JW; Petrassi, HM; Sacchettini, JC; Smith, CS; White, JT | 1 |
| Deechongkit, S; Hammarström, P; Jiang, X; Kelly, JW | 1 |
| Lee, C; Seo, EJ; Yu, MH | 1 |
| Balny, C; Carpenter, JF; Randolph, TW; St John, RJ | 1 |
| Matthews, KS; Nichols, NM | 1 |
| Hoshino, H; Takahashi, T; Yotsuyanagi, T | 1 |
| Capp, MW; Courtenay, ES; Record, MT | 1 |
| Ghosh, M; Mandal, DK | 1 |
| Chang, GG; Hung, HC | 1 |
| Ahmad, A; Bhakuni, V; Jain, SK; Prajapati, S; Prakash, K | 1 |
| Baldini, G; Collini, M; D'Alfonso, L | 1 |
| Antonin, W; Fasshauer, D; Jahn, R; Subramaniam, V | 1 |
| Barone, G; Del Vecchio, P; Granata, V; Graziano, G; Manco, G; Mandrich, L; Rossi, M | 2 |
| Sridevi, K; Udgaonkar, JB | 1 |
| Edwin, F; Jagannadham, MV; Sharma, YV | 1 |
| Al Banchaabouchi, M; D'Hooge, R; De Deyn, PP; Marescau, B | 1 |
| Ahmad, A; Akhtar, MS; Bhakuni, V | 1 |
| Aviles, FX; Chang, JY; Li, L; Salamanca, S; Vendrell, J; Villegas, V | 1 |
| Bagorogoza, K; Liavoga, A; Okot-Kotber, M; Yong, KJ | 1 |
| Biktashev, AG; Bushmarina, NA; Kuznetsova, IM; Turoverov, KK; Uversky, VN | 1 |
| Fairman, R; Ratia, K; Solan, A | 1 |
| Chang, JY; Lu, BY | 1 |
| Xu, Y; Zhou, HM; Zhou, HW | 1 |
| Kurganov, BI | 1 |
| Acevedo, O; Garcia-Mira, MM; Guzman-Casado, M; Ibarra-Molero, B; Sanchez-Ruiz, JM | 1 |
| Luo, J; Zhang, TH; Zhou, JM | 1 |
| Shi, H; Sturgis, JN; Tsang, SY; Xue, H; Zheng, H | 1 |
| Cobos, ES; Filimonov, VV; Gálvez, A; Maqueda, M; Martínez, JC; Mateo, PL; Valdivia, E | 1 |
| Balaram, H; Balaram, P; Maithal, K; Nagaraj, G; Ravindra, G; Singh, SK | 1 |
| Senthilkumar, R; Sharma, KK | 1 |
| Jagannadham, MV; Kundu, S; Sundd, M | 1 |
| Bhakuni, V; Bhatt, AN; Prakash, K; Subramanya, HS | 1 |
| Sun, CM | 1 |
| Bhuyan, AK | 1 |
| Chapeaurouge, A; Ferreira, ST; Martins, SM | 1 |
| Lilie, H; Rudolph, R; Winter, J | 1 |
| Emi, M; Hayaishi, O; Inui, T; Irikura, D; Ohkubo, T; Urade, Y | 1 |
| Berndt, KD; Chiaraluce, R; Consalvi, V; Ladenstein, R; Lebbink, JH | 1 |
| Ganesh, KN; Hegde, SS; Khan, MI; Kumar, AR | 1 |
| Balaram, H; Balaram, P; Maithal, K; Pattanaik, P; Ravindra, G; Sengupta, C | 1 |
| Borges, MH; Brito, AG; Giglio, JR; Homsi-Brandeburgo, MI; Penha-Silva, N; Soares, AM | 1 |
| Singh, S; Zlotnick, A | 1 |
| Panda, D; Santra, MK | 1 |
| Deshpande, RA; Khan, MI; Shankar, V | 1 |
| Botelho, MG; Ferreira, ST; Gralle, M; Oliveira, CL; Torriani, I | 1 |
| Schellman, JA | 1 |
| Banerjee, KK; Chattopadhyay, K | 1 |
| CREZE, J; DELAUNAY, A | 1 |
| LEONIS, J; LEVAUX, P | 1 |
| CATER, CW; NAISMITH, WE | 1 |
| HILL, RL; SCHWARTZ, HC; SMITH, EL | 1 |
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| Cabral, H; da Rosa, NG; da Silva, RR; de Oliveira, LCG; Juliano, L; Juliano, MA; Rosa, JC | 1 |
| Chi, MC; Lan, YG; Lin, LL; Lin, MG; Lo, HF; Wang, TF | 1 |
| Hughes, CS; Krijgsveld, J; Moggridge, S; Morin, GB; Müller, T; Sorensen, PH | 1 |
| Okamoto, K; Sako, Y | 1 |
| Castagnolo, D; Lauder, K; Magri, A; Mokrane, O; Patel, AH | 1 |
| Aubé, J; Bacqué, E; Ballinger, E; Blanc, I; Burns-Huang, K; Couturier, C; Engelhart, CA; Fay, A; Fraisse, L; Gold, B; Goullieux, L; Hartman, T; Ioerger, TR; Lagrange, S; McAulay, K; McConnell, JA; Morris, R; Mosior, J; Nathan, C; Ouerfelli, O; Rhee, K; Roubert, C; Sacchettini, J; Sans, S; Scarry, SM; Schnappinger, D; Vaubourgeix, J; Yang, G | 1 |
| Baker, D; Basak, S; Deveau, LM; Koga, N; Massi, F; Matthews, CR; Nobrega, RP; Tatsumi-Koga, R; Tavella, D | 1 |
| Jana, B; Parui, S | 1 |
| Ganguly, P; Shea, JE | 1 |
| Sarkar, S; Singh, PC | 1 |
| Bernabei, M; Borgini, M; Botta, M; Bugli, F; D'Agostino, I; Dreassi, E; Fiorucci, D; Khandelwal, NK; Maccari, L; Martini, C; Meis, JF; Orofino, F; Poggialini, F; Prasad, R; Sanguinetti, M; Torelli, R; Truglio, GI; Zamperini, C | 1 |
| Ahmad, F; Hassan, MI; Islam, A; Khan, SH; Lynn, AM; Pandey, P; Prakash, A | 1 |
| Hamborg, L; Horsted, EW; Johansson, KE; Lindorff-Larsen, K; Teilum, K; Willemoës, M | 1 |
| Laseke, AJ; Maurice, MS; Reiter, NJ; Schneider, NO; Tassoulas, LJ; Wackett, LP; Zeng, D | 1 |
| Gautam, BK; Kurnik, M; Ortega, G; Plaxco, KW | 1 |
| Atkinson, MJ; Schneider, M; Subedi, P; Tapio, S | 1 |
| Aukema, KG; Martinez-Vaz, B; Robinson, A; Tassoulas, LJ; Wackett, LP | 1 |
| Ahmad, F; Ahmed, A; Hassan, MI; Islam, A; Lakhrm, NA; Malik, A; Nasreen, K; Parray, ZA; Shamsi, A | 1 |
| Chakraborty, T; Hazra, J; Mitra, SK; Pal, M; Polley, S; Sau, K; Sau, S; Seal, S; Sinha, D | 1 |
| Huczyński, A; Krzywik, J; Maj, E; Mozga, W; Nasulewicz-Goldeman, A; Wietrzyk, J | 1 |
| Agrawal, S; Govind Kumar, V; Gundampati, RK; Kumar, TKS; Moradi, M | 1 |
| Elizalde-Velázquez, GA; Galar-Martínez, M; García-Medina, S; Gómez-Oliván, LM; Hernández-Navarro, MD; Islas-Flores, H | 1 |
| Anselmi, S; Biolatti, M; Castagnolo, D; Dell'Oste, V; Denaro, R; Pacheco, SFC; Toscani, A | 1 |
| Childs, EW; Jas, GS; Kuczera, K; Middaugh, CR | 1 |
| Elizalde-Velázquez, GA; Galar-Martínez, M; García-Medina, S; Gómez-Oliván, LM; Islas-Flores, H; Orozco-Hernández, JM; Rosales-Pérez, KE | 1 |
| Aubé, J; Bacqué, E; Bowler, MM; Feher, VA; Gold, BS; Goullieux, L; Lagiakos, HR; Li, K; Ling, Y; Moraca, F; Mosior, J; Nathan, CF; Ottavi, S; Perkowski, AJ; Ramesh, R; Roberts, J; Roubert, C; Sacchettini, JC; Scarry, SM; Singh, A; Tracy, W; Vendome, J; Zhang, D | 1 |
| Bhattacharya, S; Cazade, PA; Gamero-Quijano, A; Herzog, G; Scanlon, MD; Thompson, D; Walsh, S | 1 |
| Bano, B; Feroz, A; Khaki, PSS | 1 |
| Dachraoui, W; Mayer, M; Nirmalraj, PN; Rossell, MD; Thompson, D | 1 |
7 review(s) available for guanidine and urea
| Article | Year |
|---|---|
Water as ligand: preferential binding and exclusion of denaturants in protein unfolding.
Topics: Binding Sites; Guanidine; Guanidines; Protein Binding; Protein Denaturation; Protein Folding; Thermodynamics; Urea; Water | 1992 |
Urea and guanidine hydrochloride denaturation curves.
Topics: Dose-Response Relationship, Drug; Guanidine; Guanidines; Protein Denaturation; Spectrometry, Fluorescence; Thermodynamics; Urea | 1995 |
Inactivation precedes overall molecular conformation changes during enzyme denaturation.
Topics: Amino Acid Sequence; Endopeptidases; Enzyme Reactivators; Enzymes; Guanidine; Guanidines; Hot Temperature; Kinetics; Molecular Sequence Data; Protein Conformation; Protein Denaturation; Urea | 1995 |
Molten globule and protein folding.
Topics: Biological Evolution; Guanidine; Guanidines; Kinetics; Membrane Proteins; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Proteins; Urea | 1995 |
[Discovery of cis-preference of aromatic N-methylamides and its application to molecular constructions].
Topics: Amides; Crystallization; DNA; Guanidine; Molecular Conformation; Solutions; Stereoisomerism; Urea | 2001 |
Kinetics of protein aggregation. Quantitative estimation of the chaperone-like activity in test-systems based on suppression of protein aggregation.
Topics: Animals; Guanidine; Heating; Kinetics; Molecular Chaperones; Protein Denaturation; Protein Folding; Proteins; Urea | 2002 |
Role of solvation effects in protein denaturation: from thermodynamics to single molecules and back.
Topics: Fluorescence Resonance Energy Transfer; Guanidine; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteins; Solvents; Thermodynamics; Urea; Water | 2011 |
1 trial(s) available for guanidine and urea
| Article | Year |
|---|---|
Serum guanidino compound levels in uremic pediatric patients treated with hemodialysis or continuous cycle peritoneal dialysis. Correlations between nerve conduction velocities and altered guanidino compound concentrations.
Topics: Adolescent; Adult; Arginine; Child; Child, Preschool; Creatinine; Female; Guanidine; Guanidines; Humans; Male; Methylguanidine; Neural Conduction; Peritoneal Dialysis; Renal Dialysis; Succinates; Urea; Uremia | 1995 |
674 other study(ies) available for guanidine and urea
| Article | Year |
|---|---|
A relationship between the starting secondary structure of recombinant porcine growth hormone solubilised from inclusion bodies and the yield of native (monomeric) protein after in vitro refolding.
Topics: Animals; Cetrimonium; Cetrimonium Compounds; Escherichia coli; Growth Hormone; Guanidine; Guanidines; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Solubility; Swine; Urea | 1992 |
Stability, quaternary structure, and folding of internal, external, and core-glycosylated invertase from yeast.
Topics: beta-Fructofuranosidase; Enzyme Reactivators; Enzyme Stability; Glycoproteins; Glycoside Hydrolases; Glycosylation; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Protein Processing, Post-Translational; Saccharomyces cerevisiae; Urea | 1992 |
Equilibrium denaturation studies of mouse beta-nerve growth factor.
Topics: Animals; Circular Dichroism; Dose-Response Relationship, Drug; Guanidine; Guanidines; Hydrogen-Ion Concentration; Mice; Nerve Growth Factors; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Ultraviolet Rays; Urea | 1992 |
Enzyme activation by denaturants in organic solvent systems with a low water content.
Topics: Animals; Cattle; Enzyme Activation; Enzymes; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycerolphosphate Dehydrogenase; Guanidine; Guanidines; Hexokinase; Inorganic Pyrophosphatase; Isoenzymes; Kinetics; L-Lactate Dehydrogenase; Muscles; Myocardium; Protein Denaturation; Pyrophosphatases; Rabbits; Saccharomyces cerevisiae; Solutions; Solvents; Urea; Water | 1992 |
Relationship between local and global stabilities of proteins: site-directed mutants and chemically-modified derivatives of cytochrome c.
Topics: Amino Acid Sequence; Animals; Calorimetry; Cytochrome c Group; Drug Stability; Guanidine; Guanidines; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Recombinant Proteins; Urea | 1992 |
Complexoproductive and antiheparin properties of low density lipoproteins (LDL). I. Interaction of isolated low density lipoproteins with heparin of different molecular weight.
Topics: Calcium Chloride; Drug Interactions; Guanidine; Guanidines; Heparin; Heparin Antagonists; Heparin, Low-Molecular-Weight; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Indicators and Reagents; Lipoproteins, LDL; Molecular Weight; Sodium Chloride; Urea | 1992 |
Renaturation of citrate synthase: influence of denaturant and folding assistants.
Topics: Bacterial Proteins; Chaperonin 10; Chaperonin 60; Citrate (si)-Synthase; Glycerol; Guanidine; Guanidines; Heat-Shock Proteins; Models, Molecular; Oxaloacetates; Protein Denaturation; Protein Folding; Serum Albumin, Bovine; Urea | 1992 |
Unfolding intermediates of the extracellular domain of the interferon gamma receptor.
Topics: Blotting, Western; Catalysis; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanidines; Humans; Interferons; NADP; Oxidation-Reduction; Protein Conformation; Receptors, Immunologic; Receptors, Interferon; Thioredoxin-Disulfide Reductase; Thioredoxins; Urea | 1992 |
Detection of local structures in reduced unfolded bovine pancreatic trypsin inhibitor.
Topics: Aprotinin; Glycerol; Guanidine; Guanidines; Molecular Structure; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Urea; Viscosity | 1992 |
Dependence of reaction rate of 5,5'-dithiobis-(2-nitrobenzoic acid) to free sulfhydryl groups of bovine serum albumin and ovalbumin on the protein conformations.
Topics: Dithionitrobenzoic Acid; Guanidine; Guanidines; Kinetics; Mathematics; Ovalbumin; Protein Conformation; Serum Albumin, Bovine; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Urea | 1992 |
Biophysical analysis of phaseolin denaturation induced by urea, guanidinium chloride, pH, and temperature.
Topics: Circular Dichroism; Fluorescence Polarization; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Plant Proteins; Protein Denaturation; Urea | 1992 |
Conformational stability of bovine alpha-crystallin. Evidence for a destabilizing effect of ascorbate.
Topics: Acrylamide; Acrylamides; Animals; Ascorbic Acid; Cattle; Crystallins; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; In Vitro Techniques; Iodides; Light; Nephelometry and Turbidimetry; Protein Conformation; Protein Denaturation; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan; Urea | 1992 |
The uncoupling protein from brown adipose tissue mitochondria. The environment of the tryptophan residues as revealed by quenching of the intrinsic fluorescence.
Topics: Adipose Tissue, Brown; Animals; Carrier Proteins; Cricetinae; Guanidine; Guanidines; Hydrochloric Acid; Ion Channels; Mathematics; Membrane Proteins; Mitochondria; Mitochondrial Proteins; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Uncoupling Protein 1; Urea | 1992 |
Quantitative effects of antihydrophobic agents on binding constants and solubilities in water.
Topics: Alcohols; Anilino Naphthalenesulfonates; beta-Cyclodextrins; Binding Sites; Carbohydrate Conformation; Cyclodextrins; Guanidine; Guanidines; Organophosphates; Solubility; Urea; Water | 1992 |
Solubilization of growth hormone and other recombinant proteins from Escherichia coli inclusion bodies by using a cationic surfactant.
Topics: Animals; Biological Assay; Cell Fractionation; Cetrimonium; Cetrimonium Compounds; Chromatography, High Pressure Liquid; Escherichia coli; Growth Hormone; Growth Plate; Guanidine; Guanidines; Insulin-Like Growth Factor II; Interleukin-1; Protein Conformation; Recombinant Proteins; Sheep; Solubility; Surface-Active Agents; Swine; Urea | 1992 |
Stability of Arthrobacter D-xylose isomerase to denaturants and heat.
Topics: Aldose-Ketose Isomerases; Arthrobacter; Binding Sites; Carbohydrate Epimerases; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Fructose; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Macromolecular Substances; Magnesium Chloride; Molecular Weight; Protein Conformation; Protein Denaturation; Sodium Dodecyl Sulfate; Urea | 1992 |
Structure-activity relationship in buffalo spleen cathepsin B.
Topics: Animals; Binding Sites; Buffaloes; Cathepsin B; Guanidine; Guanidines; Hydrogen-Ion Concentration; In Vitro Techniques; Protein Conformation; Spleen; Structure-Activity Relationship; Urea | 1992 |
Inclusion bodies of the thermophilic endoglucanase D from Clostridium thermocellum are made of native enzyme that resists 8 M urea.
Topics: Cellulase; Circular Dichroism; Clostridium; Enzyme Activation; Guanidine; Guanidines; Hot Temperature; Protein Denaturation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Urea | 1992 |
Activity of heart and muscle lactate dehydrogenases in all-aqueous systems and in organic solvents with low amounts of water. Effect of guanidine chloride.
Topics: Animals; Cattle; Guanidine; Guanidines; Isoenzymes; Kinetics; L-Lactate Dehydrogenase; Micelles; Muscles; Myocardium; Solvents; Spectrophotometry, Ultraviolet; Swine; Urea; Water | 1992 |
Small iminium ions block gramicidin channels in lipid bilayers.
Topics: Amidines; Formamides; Gramicidin; Guanidine; Guanidines; Kinetics; Lipid Bilayers; Membrane Potentials; Models, Biological; Potassium Channels; Time Factors; Urea | 1991 |
Conformational stability of citraconylated ovalbumin.
Topics: Circular Dichroism; Citraconic Anhydrides; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanidines; Hydrogen-Ion Concentration; Ovalbumin; Protein Conformation; Spectrometry, Fluorescence; Urea | 1991 |
The quaternary structure of streptavidin in urea.
Topics: Bacterial Proteins; Biotin; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanidines; Protein Conformation; Spectrometry, Fluorescence; Streptavidin; Urea | 1991 |
Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli.
Topics: Amino Acid Sequence; Drug Stability; Escherichia coli; Fluoresceins; Genetic Vectors; Guanidine; Guanidines; Immunoglobulin Fragments; Immunoglobulins; Immunosorbent Techniques; Kinetics; Models, Molecular; Molecular Sequence Data; Plasmids; Protein Binding; Protein Conformation; Recombinant Proteins; Solvents; Urea | 1991 |
Monomer-tetramer equilibrium of the Escherichia coli ssb-1 mutant single strand binding protein.
Topics: DNA-Binding Proteins; Escherichia coli; Fluorescence Polarization; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Magnesium Chloride; Molecular Structure; Mutation; Osmolar Concentration; Sodium Chloride; Temperature; Thermodynamics; Tryptophan; Urea | 1991 |
Unfolding-refolding behaviour of chicken egg white ovomucoid and its correlation with the three domain structure of the protein.
Topics: Animals; Chickens; Egg White; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Ovomucin; Protein Conformation; Protein Denaturation; Spectrophotometry, Ultraviolet; Thermodynamics; Urea | 1991 |
Denaturation behavior of phaseolin in urea, guanidine hydrochloride, and sodium dodecyl sulfate solutions.
Topics: Fluorescence; Guanidine; Guanidines; Plant Proteins; Protein Conformation; Protein Denaturation; Sodium Dodecyl Sulfate; Urea | 1991 |
Folding of ribonuclease T1. 1. Existence of multiple unfolded states created by proline isomerization.
Topics: Endoribonucleases; Escherichia coli; Genes, Synthetic; Guanidine; Guanidines; Isomerism; Kinetics; Proline; Protein Conformation; Recombinant Proteins; Ribonuclease T1; Urea | 1990 |
Light-scattering and scanning transmission electron microscopic investigation of the hemocyanin of the bivalve, Yoldia limatula (Say).
Topics: Animals; Calcium; Chemical Phenomena; Chemistry, Physical; Circular Dichroism; Edetic Acid; Guanidine; Guanidines; Hemocyanins; Hydrogen-Ion Concentration; Light; Macromolecular Substances; Magnesium; Microscopy, Electron, Scanning; Molecular Weight; Mollusca; Scattering, Radiation; Ultracentrifugation; Urea | 1990 |
Surface tension measurements show that chaotropic salting-in denaturants are not just water-structure breakers.
Topics: Benzene; Chlorides; Ethylene Glycol; Ethylene Glycols; Formamides; Guanidine; Guanidines; Lithium; Lithium Chloride; Lithium Compounds; Perchlorates; Solubility; Surface Tension; Urea; Water | 1990 |
Characterization of biological ice nuclei from a lichen.
Topics: Bacteria; Guanidine; Guanidines; Hydrogen-Ion Concentration; Ice; Lichens; Peptide Hydrolases; Temperature; Urea | 1990 |
Reversible alkylation of an active site methionine residue in dehydroquinase.
Topics: Affinity Labels; Alkylation; Binding Sites; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Enzyme Reactivators; Escherichia coli; Guanidine; Guanidines; Hydro-Lyases; Hydrogen-Ion Concentration; Iodoacetates; Iodoacetic Acid; Kinetics; Mass Spectrometry; Mercaptoethanol; Methionine; Peptide Mapping; Protein Conformation; Protein Denaturation; Sulfides; Urea | 1990 |
Folding and unfolding of the protoxin from Bacillus thuringiensis: evidence that the toxic moiety is present in an active conformation.
Topics: Bacillus thuringiensis; Bacillus thuringiensis Toxins; Bacterial Proteins; Bacterial Toxins; Biological Assay; Endotoxins; Guanidine; Guanidines; Hemolysin Proteins; Hydrogen-Ion Concentration; Papain; Protein Conformation; Protein Denaturation; Protein Precursors; Spectrometry, Fluorescence; Structure-Activity Relationship; Trypsin; Urea | 1990 |
Association and folding in legumin oligomers of lupin seed.
Topics: Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Fabaceae; Guanidine; Guanidines; Legumins; Macromolecular Substances; Plant Proteins; Plant Proteins, Dietary; Plants, Medicinal; Protein Conformation; Protein Denaturation; Seeds; Urea | 1990 |
Serum guanidino compound levels and clearances in uremic patients treated with continuous ambulatory peritoneal dialysis.
Topics: Adult; Aged; Dialysis Solutions; Female; Guanidine; Guanidines; Humans; Kidney Failure, Chronic; Male; Middle Aged; Peritoneal Dialysis, Continuous Ambulatory; Urea; Uremia | 1990 |
Helix stability in succinylated and acetylated ovalbumins: effect of high pH, urea and guanidine hydrochloride.
Topics: Acetylation; Acylation; Anhydrides; Circular Dichroism; Guanidine; Guanidines; Hydrogen-Ion Concentration; Ovalbumin; Protein Conformation; Protein Denaturation; Urea | 1990 |
Unmasking antigen determinants in amyloid.
Topics: Amyloid; Antibodies, Monoclonal; DDT; Epitopes; Guanidine; Guanidines; Histocytochemistry; Humans; Kidney; Mercaptoethanol; Methods; Prealbumin; Urea | 1986 |
Competitive enzyme inhibition immunoassay of apolipoprotein A-I: use of monoclonal antibodies.
Topics: Antibodies, Monoclonal; Antibody Specificity; Apolipoprotein A-I; Apolipoproteins A; Drug Stability; Enzyme-Linked Immunosorbent Assay; Epitopes; Female; Guanidine; Guanidines; Hot Temperature; Humans; Immunoassay; Male; Quality Control; Statistics as Topic; Urea | 1988 |
Urea-induced inactivation, dissociation, and unfolding of the allosteric phosphofructokinase from Escherichia coli.
Topics: Allosteric Regulation; Chromatography, High Pressure Liquid; Escherichia coli; Guanidine; Guanidines; Kinetics; Phosphofructokinase-1; Protein Conformation; Protein Denaturation; Thiocyanates; Tryptophan; Urea | 1989 |
Effects of denaturants at low concentrations on the reversible denaturation of staphylococcal nuclease.
Topics: Anions; Binding Sites; Calcium; Glycerol; Guanidine; Guanidines; Iodides; Micrococcal Nuclease; Mutation; Perchlorates; Protein Conformation; Protein Denaturation; Sodium Compounds; Spectrometry, Fluorescence; Thermodynamics; Thiocyanates; Urea | 1989 |
Structural transitions of carboxymethylated cytochrome c: calorimetric and circular dichroic studies.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Cytochrome c Group; Guanidine; Guanidines; Kinetics; Methionine; Methylation; Protein Conformation; Protein Denaturation; Urea | 1989 |
Apoptotic hepatocytes become insoluble in detergents and chaotropic agents as a result of transglutaminase action.
Topics: Animals; Detergents; Dipeptides; DNA; Guanidine; Guanidines; Hot Temperature; Liver; Male; Mercaptoethanol; Microscopy, Electron, Scanning; Rats; Rats, Inbred Strains; Sodium Dodecyl Sulfate; Solubility; Spermidine; Surface-Active Agents; Transglutaminases; Urea | 1989 |
Circular dichroic study of conformational changes in ovalbumin induced by modification of sulfhydryl groups and disulfide reduction.
Topics: Circular Dichroism; Disulfides; Guanidine; Guanidines; Ovalbumin; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Sodium Dodecyl Sulfate; Sulfhydryl Compounds; Urea | 1989 |
Local phenomena and distribution of molecular species during the unfolding of heme-free myoglobin in the presence of GdnHCl and urea as seen by time-resolved fluorescence spectroscopy.
Topics: Apoproteins; Guanidine; Guanidines; Kinetics; Myoglobin; Protein Denaturation; Spectrometry, Fluorescence; Time Factors; Urea | 1989 |
Characterization of lens alpha-crystallin tryptophan microenvironments by room temperature phosphorescence spectroscopy.
Topics: Acrylamide; Acrylamides; Crystallins; Guanidine; Guanidines; Iodides; Luminescent Measurements; Nitrites; Protein Conformation; Protein Denaturation; Spectrum Analysis; Temperature; Thermodynamics; Tryptophan; Urea | 1989 |
Bovine liver formaldehyde dehydrogenase. Kinetic and molecular properties.
Topics: Aldehyde Oxidoreductases; Animals; Binding Sites; Catalysis; Cattle; Deuterium; Guanidine; Guanidines; Humans; Isoelectric Point; Isoenzymes; Kinetics; Liver; Molecular Weight; NAD; Protein Denaturation; Urea | 1989 |
Secondary structural changes in the intact and the disulfide bridges cleaved beta-lactoglobulin A and B in solutions of urea, guanidine hydrochloride, and sodium dodecyl sulfate.
Topics: Circular Dichroism; Guanidine; Guanidines; Lactoglobulins; Protein Conformation; Sodium Dodecyl Sulfate; Solutions; Urea | 1989 |
Isolation and separation of physicochemically distinct fimbrial types expressed on a single culture of Escherichia coli O7:K1:H6.
Topics: Bacterial Proteins; Escherichia coli; Fimbriae, Bacterial; Glucosides; Guanidine; Guanidines; Urea | 1985 |
Effects of urea and guanidine hydrochloride on the activity and dynamical structure of equine liver alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Animals; Circular Dichroism; Guanidine; Guanidines; Horses; Kinetics; Liver; Luminescent Measurements; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Urea; Viscosity | 1986 |
Refolding a disulfide dimer of cytochrome c.
Topics: Circular Dichroism; Cytochrome c Group; Disulfides; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Protein Conformation; Protein Denaturation; Saccharomyces cerevisiae; Urea | 1985 |
Solubilization of peripheral benzodiazepine-binding sites from rat kidney.
Topics: Animals; Benzodiazepinones; Binding, Competitive; Carbolines; Chlordiazepoxide; Clonazepam; Diazepam; Digitonin; Flumazenil; Flurazepam; Guanidine; Guanidines; Hot Temperature; Isoquinolines; Kidney; Kinetics; Male; Rats; Rats, Inbred Strains; Receptors, GABA-A; Solubility; Time Factors; Urea | 1985 |
Dissociation of newly synthesized Sendai viral proteins from the cytoplasmic surface of isolated plasma membranes of infected cells.
Topics: Animals; Cell Membrane; Cricetinae; Cytoplasm; Guanidine; Guanidines; Membrane Proteins; Molecular Weight; Parainfluenza Virus 1, Human; Potassium Chloride; Protein Denaturation; Solubility; Thiocyanates; Urea; Viral Envelope Proteins | 1986 |
Investigations of primary and secondary structure of porcine ubiquitin. Its N epsilon-acetylated lysine derivative.
Topics: Acetylation; Amino Acid Sequence; Animals; Cattle; Circular Dichroism; Dithiothreitol; Erythrocytes; Guanidine; Guanidines; High Mobility Group Proteins; Lysine; Male; Protein Conformation; Swine; Ubiquitins; Urea | 1986 |
Inorganic phosphate assay with the ascorbic acid or stannous chloride method: influence of fluoride.
Topics: Ascorbic Acid; Carbonates; Colorimetry; Edetic Acid; Fluorides; Guanidine; Guanidines; Phosphates; Proteins; Tin; Tin Compounds; Urea | 1986 |
Hemocyanin of the chiton, Stenoplax conspicua (Dall).
Topics: Animals; Edetic Acid; Guanidine; Guanidines; Hemocyanins; Hydrogen-Ion Concentration; Kinetics; Macromolecular Substances; Magnesium; Mathematics; Molecular Weight; Mollusca; Protein Conformation; Urea | 1987 |
Secondary structural changes of non-reduced and reduced ribonuclease A in solutions of urea, guanidine hydrochloride and sodium dodecyl sulfate.
Topics: Circular Dichroism; Disulfides; Dose-Response Relationship, Drug; Guanidine; Guanidines; Oxidation-Reduction; Protein Conformation; Ribonuclease, Pancreatic; Sodium Dodecyl Sulfate; Solutions; Urea | 1988 |
Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants.
Topics: Binding Sites; Chymotrypsin; Guanidine; Guanidines; Kinetics; Mathematics; Methylurea Compounds; Phenylmethylsulfonyl Fluoride; Protein Conformation; Protein Denaturation; Solvents; Thermodynamics; Urea | 1988 |
Effect of urea, guanidine hydrochloride and sodium dodecyl sulphate on the isolated major protein fraction, lupinin, from lupin seed (Lupinus angustifolius).
Topics: Guanidine; Guanidines; Plant Proteins; Protein Conformation; Seeds; Sodium Dodecyl Sulfate; Urea | 1988 |
L1210 dihydrofolate reductase. Kinetics and mechanism of activation by various agents.
Topics: Animals; Cell Line; Chloromercuribenzoates; Dithionitrobenzoic Acid; Enzyme Activation; Guanidine; Guanidines; Kinetics; Leukemia L1210; Mice; p-Chloromercuribenzoic Acid; Peptide Hydrolases; Potassium Chloride; Protein Conformation; Sodium Chloride; Spectrometry, Fluorescence; Tetrahydrofolate Dehydrogenase; Urea | 1987 |
Destabilization of lens protein conformation by glutathione mixed disulfide.
Topics: Animals; Cattle; Crystallins; Disulfides; Glutathione; Guanidine; Guanidines; Protein Conformation; Protein Denaturation; Time Factors; Urea | 1988 |
Rat brain hexokinase: location of the allosteric regulatory site in a structural domain at the N-terminus of the enzyme.
Topics: Animals; Binding Sites; Brain; Glucose-6-Phosphate; Glucosephosphates; Guanidine; Guanidines; Hexokinase; Hexoses; Peptide Mapping; Rats; Urea | 1987 |
Biodegradation of guanidinium ion in aerobic soil samples.
Topics: Aerobiosis; Biodegradation, Environmental; Guanidine; Guanidines; Soil; Sterilization; Urea | 1987 |
Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation.
Topics: Circular Dichroism; Guanidine; Guanidines; Micrococcal Nuclease; Mutation; Protein Conformation; Protein Denaturation; Urea | 1986 |
Structure of unfolded and refolded recombinant derived [Ala125]interleukin 2.
Topics: Acetates; Circular Dichroism; Disulfides; Guanidine; Guanidines; Interleukin-2; Protein Conformation; Protein Denaturation; Recombinant Proteins; Urea | 1986 |
Intramolecular disulfide loop formation in a peptide containing two cysteines.
Topics: Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Glutathione; Guanidine; Guanidines; Iodoacetates; Kinetics; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Trypsin Inhibitor, Kunitz Soybean; Trypsin Inhibitors; Urea | 1987 |
"4 S" polycyclic aromatic hydrocarbon binding protein: further characterization and kinetic properties.
Topics: Animals; Cytosol; Female; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Liver; Polycyclic Compounds; Protein Binding; Rats; Rats, Inbred Strains; Temperature; Urea | 1987 |
Hydrophobic stabilization of chiton hemocyanins: effects of ureas, Hofmeister salts and pH on their dissociation.
Topics: Animals; Chlorates; Circular Dichroism; Guanidine; Guanidines; Hemocyanins; Hydrogen-Ion Concentration; Light; Macromolecular Substances; Molecular Weight; Mollusca; Salts; Scattering, Radiation; Sodium Iodide; Thiocyanates; Ultracentrifugation; Urea | 1987 |
Model risk analysis of nitrosatable compounds in the diet as precursors of potential endogenous carcinogens.
Topics: Amines; Amino Acids; Biotransformation; Carcinogens; Diet; Dietary Proteins; Dimethylnitrosamine; Guanidine; Guanidines; Humans; Kinetics; Models, Statistical; Nitroso Compounds; Risk; Urea | 1987 |
Studies on the quaternary structure of class I major histocompatibility complex antigens. Effect of different agents on the interaction between subunits.
Topics: Animals; Detergents; Guanidine; Guanidines; Histocompatibility Antigens; HLA Antigens; Humans; Hydrogen-Ion Concentration; Macromolecular Substances; Mice; Temperature; Urea | 1986 |
Determination and analysis of urea and guanidine hydrochloride denaturation curves.
Topics: Guanidine; Guanidines; Kinetics; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Urea | 1986 |
Ultrasonic absorption of bovine serum albumin solutions in the frequency range 60 to 160 kHz.
Topics: Animals; Cattle; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Serum Albumin; Sodium Dodecyl Sulfate; Ultrasonics; Urea | 1986 |
Subunit dissociation of Busycon canaliculatum hemocyanin.
Topics: Animals; Circular Dichroism; Copper; Guanidine; Guanidines; Hemocyanins; Hydrogen-Ion Concentration; Light; Macromolecular Substances; Molecular Weight; Protein Denaturation; Scattering, Radiation; Snails; Urea | 1985 |
Use of a urea and guanidine-HCl-propanol solvent system to purify a growth inhibitory glycopeptide by high-performance liquid chromatography.
Topics: 1-Propanol; Animals; Cattle; Cells, Cultured; Chromatography, High Pressure Liquid; Drug Stability; Growth Inhibitors; Guanidine; Guanidines; Membrane Proteins; Mice; Mice, Inbred BALB C; Solvents; Urea | 1985 |
Light-scattering investigation of the dissociation behavior of Lunatia heros and Littorina littorea hemocyanins.
Topics: Animals; Circular Dichroism; Guanidine; Guanidines; Hemocyanins; Hydrogen-Ion Concentration; Light; Macromolecular Substances; Molecular Weight; Protein Conformation; Scattering, Radiation; Snails; Species Specificity; Urea | 1985 |
Kinetics and mechanism of the refolding of denatured ribonuclease A.
Topics: Animals; Cattle; Drug Stability; Guanidine; Guanidines; Kinetics; Mathematics; Models, Biological; Pancreas; Protein Conformation; Protein Denaturation; Ribonuclease, Pancreatic; Urea | 1985 |
Calculation of the partial specific volume of proteins in concentrated salt and amino acid solutions.
Topics: Amino Acids; Ammonium Sulfate; Guanidine; Guanidines; Molecular Weight; Muramidase; Osmolar Concentration; Protein Conformation; Proteins; Solutions; Urea | 1985 |
Untangling insoluble filaments.
Topics: Acyltransferases; Alzheimer Disease; Dementia; Guanidine; Guanidines; Humans; Neurofibrils; Octoxynol; Polyethylene Glycols; Sodium Dodecyl Sulfate; Solubility; Transglutaminases; Urea | 1983 |
Location of guanidino and ureido groups in bluensomycin from C NMR spectra of streptomycin and related compounds.
Topics: Chemical Phenomena; Chemistry; Dihydrostreptomycin Sulfate; Guanidine; Guanidines; Magnetic Resonance Spectroscopy; Streptomycin; Urea | 1982 |
Solubilization, isolation, and immunochemical characterization of the major outer membrane protein from Rhodopseudomonas sphaeroides.
Topics: Bacterial Proteins; Electrophoresis, Polyacrylamide Gel; Epitopes; Guanidine; Guanidines; Immunologic Techniques; Macromolecular Substances; Membrane Proteins; Rhodobacter sphaeroides; Sodium Dodecyl Sulfate; Solubility; Urea | 1983 |
One-step molybdate method for rapid determination of inorganic phosphate in the presence of protein.
Topics: Adenosine Triphosphate; Amino Acids; Animals; Chemical Phenomena; Chemistry; Colorimetry; Diphosphates; Guanidine; Guanidines; Molybdenum; Phosphates; Proteins; Rabbits; Time Factors; Urea | 1981 |
Purification and some properties of myeloperoxidase and eosinophil peroxidase from human blood.
Topics: Chromatography, Affinity; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Eosinophils; Guanidine; Guanidines; Hot Temperature; Humans; Isoenzymes; Peroxidase; Peroxidases; Spectrophotometry; Urea | 1983 |
The stability of solubilized mammalian muscle acetylcholine receptors during purification by monoclonal immunoadsorption.
Topics: Animals; Antibodies, Monoclonal; Female; Guanidine; Guanidines; Hydrogen-Ion Concentration; Immunosorbent Techniques; Muscles; Receptors, Cholinergic; Solubility; Torpedo; Urea | 1984 |
Physical and chemical properties of extracellular staphylococcal lipase.
Topics: Guanidine; Guanidines; Lipase; Oils; Osmolar Concentration; Peptide Hydrolases; Polysorbates; Sodium Chloride; Staphylococcus; Substrate Specificity; Urea | 1983 |
The denaturation of rabbit muscle phosphorylase b by guanidinium chloride.
Topics: Animals; Circular Dichroism; Guanidine; Guanidines; Muscles; Phosphorylase b; Phosphorylases; Protein Conformation; Protein Denaturation; Rabbits; Spectrophotometry; Subtilisins; Sulfhydryl Compounds; Urea | 1983 |
Light-scattering investigation of the subunit structure and dissociation of Helix pomatia hemocyanin. Effects of salts and ureas.
Topics: Animals; Guanidine; Guanidines; Helix, Snails; Hemocyanins; Kinetics; Light; Macromolecular Substances; Mathematics; Osmolar Concentration; Salts; Scattering, Radiation; Urea | 1984 |
Effects of urea and guanidine hydrochloride on peptide and nonpolar groups.
Topics: Energy Transfer; Esters; Glycine; Guanidine; Guanidines; Peptides; Protein Denaturation; Solubility; Thermodynamics; Urea | 1984 |
Covalent DNA-protein crosslinking occurs after hyperthermia and radiation.
Topics: Animals; Cell Line; DNA; Dose-Response Relationship, Radiation; Guanidine; Guanidines; Hot Temperature; Hydroxylamine; Hydroxylamines; Leukemia L1210; Mice; Proteins; Time Factors; Urea | 1983 |
Effects of chlorhexidine and four antimicrobial compounds on plaque, gingivitis, and staining in beagle dogs.
Topics: Adamantane; Animals; Chlorhexidine; Dental Plaque; Disinfectants; Dogs; Female; Gingival Crevicular Fluid; Gingivitis; Guanidine; Guanidines; Male; Piperazines; Tooth Discoloration; Urea | 1982 |
Resistance to deactivation of enzyme-collagen membranes.
Topics: Aspartate Aminotransferases; Collagen; Enzymes, Immobilized; Glutaral; Guanidine; Guanidines; Membranes, Artificial; Protein Denaturation; Temperature; Time Factors; Urea | 1980 |
Jack been urease (EC 3.5.1.5). II. The relationship between nickel, enzymatic activity, and the "abnormal" ultraviolet spectrum. The nickel content of jack beans.
Topics: Diacetyl; Edetic Acid; Fabaceae; Guanidine; Guanidines; Hydrogen-Ion Concentration; Nickel; Oximes; Plants; Plants, Medicinal; Spectrophotometry, Ultraviolet; Urea; Urease | 1980 |
On the denaturation of porcine erythrocyte catalase with alkali, urea, and guanidine hydrochloride in relation to its subunit structure.
Topics: Alkalies; Animals; Catalase; Chemical Phenomena; Chemistry; Circular Dichroism; Erythrocytes; Guanidine; Guanidines; In Vitro Techniques; Peptide Fragments; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Swine; Urea | 1983 |
Free energy changes in ribonuclease A denaturation. Effect of urea, guanidine hydrochloride, and lithium salts.
Topics: Animals; Cattle; Chemical Phenomena; Chemistry; Circular Dichroism; Endoribonucleases; Guanidine; Guanidines; Lithium; Mathematics; Pancreas; Protein Denaturation; Ribonuclease, Pancreatic; Urea | 1983 |
Stepwise dissociation/denaturation and reassociation/renaturation of bovine alpha-crystallin in urea and guanidine hydrochloride: sedimentation, fluorescence, near-ultraviolet and far-ultraviolet circular dichroism studies.
Topics: Animals; Cattle; Circular Dichroism; Crystallins; Guanidine; Guanidines; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Ultracentrifugation; Urea | 1982 |
Preparation and fractionation of myosin light chains and exchange of the essential light chains.
Topics: Animals; Drug Stability; Guanidine; Guanidines; Methods; Molecular Weight; Muscles; Myosins; Organ Specificity; Species Specificity; Urea | 1982 |
[Hypnotic drugs. 2. Reaction of guanidine carboxamide with fluorinated beta-diketones].
Topics: Chemical Phenomena; Chemistry; Guanidine; Hydrocarbons, Fluorinated; Hypnotics and Sedatives; Urea | 1981 |
Tightly bound non-histone proteins in nucleosomes from pig-liver chromatin.
Topics: Animals; Chromosomal Proteins, Non-Histone; DNA; Guanidine; Guanidines; Hydroxyapatites; Liver; Micrococcal Nuclease; Molecular Weight; Nucleosomes; Swine; Urea | 1981 |
The binding of [3H]oestradiol-receptor complexes to calf uterine chromatin.
Topics: Animals; Binding Sites; Cattle; Cellulose; Chromatin; Estradiol; Female; Guanidine; Guanidines; In Vitro Techniques; Protein Binding; Receptors, Estradiol; Receptors, Estrogen; Sodium Chloride; Urea; Uterus | 1981 |
Sucrose reduces the efficiency of protein denaturation by a chaotropic agent.
Topics: Formamides; Geobacillus stearothermophilus; Guanidine; Guanidines; Kinetics; Phosphoglycerate Kinase; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrum Analysis, Raman; Sucrose; Thermodynamics; Urea; Water | 1995 |
Purification and characterization of allantoate amidohydrolase from Bacillus fastidiosus.
Topics: Allantoin; Bacillus; Diethyl Pyrocarbonate; Enzyme Activation; Guanidine; Guanidines; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Isoelectric Point; Kinetics; Protein Conformation; Protein Denaturation; Urea; Ureohydrolases | 1995 |
Structural characterization of the FK506 binding protein unfolded in urea and guanidine hydrochloride.
Topics: Amino Acid Sequence; Carrier Proteins; Guanidine; Guanidines; Heat-Shock Proteins; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Tacrolimus; Tacrolimus Binding Proteins; Thermodynamics; Urea | 1994 |
The physical properties of local interactions of tyrosine residues in peptides and unfolded proteins.
Topics: Amino Acid Sequence; Aprotinin; Chemical Phenomena; Chemistry, Physical; Guanidine; Guanidines; Magnetic Resonance Spectroscopy; Models, Chemical; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Folding; Proteins; Tyrosine; Urea | 1995 |
The use of preparative polyacrylamide gel electrophoresis and electroelution for purification of mucus glycoproteins.
Topics: Acetylgalactosamine; Acetylglucosamine; Animals; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Fucose; Galactose; Gastric Mucins; Guanidine; Guanidines; Mannose; Mucus; Sodium Dodecyl Sulfate; Swine; Urea | 1995 |
Chaotropic agents cause disaggregation and enhanced activity of Pasteurella haemolytica leukotoxin.
Topics: Animals; Bacterial Toxins; Cattle; Guanidine; Guanidines; L-Lactate Dehydrogenase; Lymphoma; Mannheimia haemolytica; Thiocyanates; Tumor Cells, Cultured; Urea | 1995 |
The hemocyanin of the Californian black sea hare, Aplysia vaccaria Winkler.
Topics: Animals; Aplysia; Guanidine; Guanidines; Hemocyanins; Hemolymph; Hydrogen-Ion Concentration; Light; Microscopy, Electron, Scanning Transmission; Molecular Weight; Protein Conformation; Scattering, Radiation; Urea | 1995 |
The effects of interhelical electrostatic repulsions between glutamic acid residues in controlling the dimerization and stability of two-stranded alpha-helical coiled-coils.
Topics: Amino Acid Sequence; Chromatography, Gel; Circular Dichroism; Disulfides; Glutamic Acid; Guanidine; Guanidines; Hydrogen-Ion Concentration; Models, Molecular; Molecular Sequence Data; Oxidation-Reduction; Peptides; Potassium Chloride; Protein Binding; Protein Denaturation; Protein Structure, Secondary; Scattering, Radiation; Thermodynamics; Ultracentrifugation; Urea | 1995 |
The properties of scrambled hirudins.
Topics: Chromatography, High Pressure Liquid; Disulfides; Guanidine; Guanidines; Hirudins; Oxidation-Reduction; Peptide Fragments; Protein Denaturation; Protein Folding; Serine Endopeptidases; Urea | 1995 |
Thermodynamic stability of the molten globule states of apomyoglobin.
Topics: Animals; Apoproteins; Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Guanidines; Horses; Myoglobin; Protein Denaturation; Protein Folding; Thermodynamics; Urea | 1995 |
A peptide fragment of human DNA topoisomerase II alpha forms a stable coiled-coil structure in solution.
Topics: Amino Acid Sequence; Circular Dichroism; DNA Topoisomerases, Type II; Glutaral; Guanidine; Guanidines; Humans; Molecular Sequence Data; Peptide Fragments; Protein Folding; Solutions; Trifluoroethanol; Urea | 1995 |
Multiple-state equilibrium unfolding of guanidino kinases.
Topics: Animals; Arginine Kinase; Chemical Phenomena; Chemistry, Physical; Chickens; Chromatography, High Pressure Liquid; Circular Dichroism; Creatine Kinase; Cytosol; Guanidine; Guanidines; Macromolecular Substances; Mitochondria; Molecular Weight; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Urea | 1995 |
Denaturation of pteroylpoly-gamma-glutamyl hydrolase from chicken liver by urea, thiourea and guanidine hydrochloride: altered catalytic properties of the enzyme activated by urea.
Topics: Animals; Catalysis; Chickens; Enzyme Activation; gamma-Glutamyl Hydrolase; Guanidine; Guanidines; Liver; Protein Denaturation; Thiourea; Urea | 1995 |
Stability and kinetics of a bifunctional amylase/trypsin inhibitor.
Topics: Amino Acid Sequence; Amylases; Circular Dichroism; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Panicum; Protein Denaturation; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Trypsin; Trypsin Inhibitors; Urea | 1993 |
Protein denaturation with guanidine hydrochloride or urea provides a different estimate of stability depending on the contributions of electrostatic interactions.
Topics: Amino Acid Sequence; Circular Dichroism; Guanidine; Guanidines; Molecular Sequence Data; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Thermodynamics; Urea | 1994 |
Role of disulfide linkages in structure and activity of proteinase inhibitor from horsegram (Dolichos biflorus).
Topics: Disulfides; Dithiothreitol; Fabaceae; Guanidine; Guanidines; Oxidation-Reduction; Plants, Medicinal; Protease Inhibitors; Protein Folding; Urea | 1995 |
Denaturation of cytochrome P450 2B1 by guanidine hydrochloride and urea: evidence for a metastable intermediate state of the active site.
Topics: Animals; Aryl Hydrocarbon Hydroxylases; Binding Sites; Cytochrome P-450 Enzyme System; Guanidine; Guanidines; Kinetics; Microsomes, Liver; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Rats; Sodium Chloride; Spectrophotometry; Steroid Hydroxylases; Urea | 1995 |
Domain structure, stability and domain-domain interactions in recombinant factor XIII.
Topics: Calorimetry, Differential Scanning; Factor XIII; Guanidine; Guanidines; Hot Temperature; Humans; Hydrogen-Ion Concentration; Models, Molecular; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Ultracentrifugation; Urea | 1995 |
The molecular weight and subunit organization of Helisoma trivolvis (Say) hemoglobin: light-scattering and scanning transmission electron microscopic studies.
Topics: Animals; Chemical Phenomena; Chemistry, Physical; Disulfides; Guanidine; Guanidines; Hemoglobins; Light; Macromolecular Substances; Microscopy, Electron, Scanning Transmission; Molecular Weight; Protein Denaturation; Scattering, Radiation; Snails; Urea | 1994 |
The refolding of hen egg white riboflavin binding protein.
Topics: Animals; Carrier Proteins; Chickens; Dithiothreitol; Edetic Acid; Female; Guanidine; Guanidines; Membrane Transport Proteins; Phosphorylation; Protein Conformation; Protein Denaturation; Protein Folding; Riboflavin; Spectrometry, Fluorescence; Tryptophan; Urea | 1995 |
Process of thermal denaturation of xylanase (XynB) from Clostridium stercorarium F-9.
Topics: Calorimetry, Differential Scanning; Clostridium; Endo-1,4-beta Xylanases; Enzyme Stability; Escherichia coli; Glucosides; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Protein Denaturation; Recombinant Proteins; Substrate Specificity; Urea; Xylosidases | 1995 |
Denaturation of horseradish peroxidase with urea and guanidine hydrochloride.
Topics: Binding Sites; Chemical Phenomena; Chemistry, Physical; Guanidine; Guanidines; Horseradish Peroxidase; Hydrogen-Ion Concentration; Macromolecular Substances; Models, Chemical; Protein Denaturation; Thermodynamics; Urea | 1995 |
Cold denaturation of CheY.
Topics: Bacterial Proteins; Chemotaxis; Circular Dichroism; Cold Temperature; Drug Stability; Escherichia coli; Escherichia coli Proteins; Guanidine; Guanidines; Membrane Proteins; Methyl-Accepting Chemotaxis Proteins; Models, Chemical; Protein Denaturation; Salmonella typhimurium; Thermodynamics; Urea | 1995 |
Monovalent amidiniums block calcium channels in chick sensory neurons.
Topics: Amidines; Animals; Arginine; Barium; Calcium Channel Blockers; Calcium Channels; Chickens; Ganglia, Spinal; Glucose; Guanidine; Guanidines; In Vitro Techniques; Kinetics; Membrane Potentials; Neurons, Afferent; Sodium; Structure-Activity Relationship; Thermodynamics; Urea | 1994 |
Evidence for residual structures in an unfolded form of yeast phosphoglycerate kinase.
Topics: Guanidine; Guanidines; Mutagenesis, Site-Directed; Phenylalanine; Phosphoglycerate Kinase; Protein Conformation; Protein Folding; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 1995 |
Hydrophobic surfaces that are hidden in chaperonin Cpn60 can be exposed by formation of assembly-competent monomers or by ionic perturbation of the oligomer.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Chaperonin 60; Chymotrypsin; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Macromolecular Substances; Osmolar Concentration; Peptide Fragments; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Surface Properties; Ultracentrifugation; Urea | 1995 |
Stability of the asymmetric Escherichia coli chaperonin complex. Guanidine chloride causes rapid dissociation.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Chaperonin 10; Chaperonin 60; Escherichia coli; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Models, Theoretical; Osmolar Concentration; Potassium Chloride; Protein Folding; Recombinant Proteins; Ribulose-Bisphosphate Carboxylase; Urea | 1995 |
Reversible dissociation and unfolding of pyruvate decarboxylase from Zymomonas mobilis.
Topics: Amino Acid Sequence; Chromatography, Gel; Circular Dichroism; Conserved Sequence; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Molecular Sequence Data; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Pyruvate Decarboxylase; Recombinant Proteins; Sequence Homology, Amino Acid; Urea; Zymomonas | 1994 |
Analysis of RNase A refolding intermediates by electrospray/mass spectrometry.
Topics: Animals; Cattle; Guanidine; Guanidines; Kinetics; Mass Spectrometry; Pancreas; Protein Denaturation; Protein Folding; Ribonuclease, Pancreatic; Sulfhydryl Compounds; Sulfhydryl Reagents; Time Factors; Urea | 1994 |
A kinetic method to evaluate the two-state character of solvent-induced protein denaturation.
Topics: Circular Dichroism; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Lactalbumin; Protein Denaturation; Protein Folding; Ribonuclease T1; Sodium Chloride; Solvents; Spectrometry, Fluorescence; Urea | 1994 |
Conformational stability of alpha-lactalbumin missing a peptide bond between Asp66 and Pro67.
Topics: Amino Acid Sequence; Aspartic Acid; Circular Dichroism; Disulfides; Drug Stability; Ethanol; Formates; Guanidine; Guanidines; Lactalbumin; Proline; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Spectrometry, Fluorescence; Thermodynamics; Urea | 1994 |
Effect of environment on insulin-like growth factor I refolding selectivity.
Topics: Amino Acid Sequence; Guanidine; Guanidines; Insulin-Like Growth Factor I; Molecular Sequence Data; Osmolar Concentration; Protein Denaturation; Protein Folding; Recombinant Proteins; Salts; Solutions; Temperature; Urea | 1994 |
Physical characterization of the flagella and flagellins from Methanospirillum hungatei.
Topics: Amino Acid Sequence; Cell Fractionation; Cyanogen Bromide; Detergents; Edetic Acid; Endopeptidases; Flagella; Flagellin; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Methanomicrobiales; Microscopy, Electron; Molecular Sequence Data; Negative Staining; Peptide Fragments; Sequence Analysis; Urea | 1994 |
Stability and structural analysis of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23.
Topics: alpha-Amylases; Amino Acid Sequence; Animals; Antarctic Regions; Enzyme Stability; Gram-Negative Aerobic Bacteria; Guanidine; Guanidines; Kinetics; Molecular Sequence Data; Pancreas; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Sequence Homology, Amino Acid; Swine; Thermodynamics; Urea | 1994 |
Relationship between functional properties and structure of ovalbumin.
Topics: Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanidines; Kinetics; Ovalbumin; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Urea | 1994 |
Electrospray ionization mass spectrometry as a mechanistic tool: mass of human leucocyte elastase and a beta-lactam-derived E-I complex.
Topics: Anti-Bacterial Agents; Azetidines; beta-Lactams; Binding Sites; Guanidine; Guanidines; Humans; Isoenzymes; Leukocyte Elastase; Macromolecular Substances; Molecular Weight; Oligopeptides; Pancreatic Elastase; Proteinase Inhibitory Proteins, Secretory; Proteins; Secretory Leukocyte Peptidase Inhibitor; Serine Proteinase Inhibitors; Spectrum Analysis; Urea | 1993 |
Active site structure and stability of the thiol protease papain studied by electron paramagnetic resonance employing a methanethiosulfonate spin label.
Topics: Binding Sites; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Guanidine; Guanidines; Mesylates; Papain; Protein Denaturation; Spin Labels; Urea | 1994 |
Evaluation of protein-denaturing immunoassays for avidity of immunoglobulin G to rubella virus.
Topics: Antibody Affinity; Diethylamines; Enzyme-Linked Immunosorbent Assay; Guanidine; Guanidines; Humans; Hydrogen-Ion Concentration; Immunoglobulin G; Protein Denaturation; Rubella; Rubella virus; Thiocyanates; Urea | 1994 |
Refolding of the isolated extracellular domain of the erythropoietin receptor.
Topics: Animals; Cell Line; Chlorocebus aethiops; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Erythropoietin; Guanidine; Guanidines; Mice; Molecular Weight; Protein Denaturation; Protein Folding; Receptors, Erythropoietin; Recombinant Proteins; Transfection; Urea | 1994 |
Erythrina caffra trypsin inhibitor retains its native structure and function after reducing its disulfide bonds.
Topics: Chymotrypsin; Disulfides; Drug Stability; Electrophoresis, Polyacrylamide Gel; Erythrina; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Molecular Weight; Oxidation-Reduction; Plant Proteins; Plants, Medicinal; Protein Denaturation; Protein Folding; Seeds; Spectrophotometry, Ultraviolet; Trypsin; Trypsin Inhibitors; Urea | 1994 |
A new method for testing the functional dependence of unfolding free energy changes on denaturant concentration.
Topics: Animals; Cytochrome c Group; Enzyme Stability; Guanidine; Guanidines; Horses; Muramidase; Myocardium; Protein Denaturation; Protein Folding; Ribonuclease, Pancreatic; Thermodynamics; Urea | 1994 |
Reduced binding capacity of concanavalin A-sepharose after treatment with chaotropic agents.
Topics: Animals; Buffers; Chromatography, Affinity; Guanidine; Guanidines; Interferon-gamma; Mice; Octoxynol; Protein Denaturation; Sepharose; Sodium Dodecyl Sulfate; Urea | 1993 |
Use of fast protein size-exclusion liquid chromatography to study the unfolding of proteins which denature through the molten globule.
Topics: Chromatography, High Pressure Liquid; Chromatography, Liquid; Guanidine; Guanidines; Molecular Weight; Protein Denaturation; Proteins; Solvents; Urea | 1993 |
Stabilization of creatinase from Pseudomonas putida by random mutagenesis.
Topics: Cloning, Molecular; Enzyme Activation; Enzyme Stability; Escherichia coli; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Mutagenesis; Nitrosoguanidines; Protein Denaturation; Pseudomonas putida; Thermodynamics; Urea; Ureohydrolases | 1993 |
Thermodynamics of unfolding for turkey ovomucoid third domain: thermal and chemical denaturation.
Topics: Animals; Circular Dichroism; Dose-Response Relationship, Drug; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Models, Chemical; Ovomucin; Peptide Fragments; Protein Denaturation; Spectrophotometry, Ultraviolet; Thermodynamics; Turkeys; Urea | 1993 |
Correlation of high-temperature stability of alpha-chymotrypsin with 'salting-in' properties of solution.
Topics: Chymotrypsin; Enzyme Stability; Formamides; Guanidine; Guanidines; Hot Temperature; Kinetics; Models, Structural; Osmolar Concentration; Protein Conformation; Solutions; Thermodynamics; Thiocyanates; Urea | 1994 |
Tryptophan phosphorescence as a structural probe of mitochondrial F1-ATPase epsilon-subunit.
Topics: Adenosine Triphosphate; Animals; Cattle; Cold Temperature; Guanidine; Guanidines; Kinetics; Luminescent Measurements; Mitochondria, Heart; Nucleotides; Proton-Translocating ATPases; Spectrophotometry; Tryptophan; Urea | 1993 |
Multiple active conformers of mouse ornithine decarboxylase.
Topics: Animals; Binding Sites; Decarboxylation; Eflornithine; Guanidine; Guanidines; Mice; Mutation; Ornithine Decarboxylase; Protein Conformation; Protein Denaturation; Recombinant Proteins; Urea | 1993 |
Structure-function relations in photosystem II. Effects of temperature and chaotropic agents on the period four oscillation of flash-induced oxygen evolution.
Topics: Guanidine; Guanidines; Kinetics; Oxidation-Reduction; Oxygen; Photolysis; Photosynthetic Reaction Center Complex Proteins; Photosystem II Protein Complex; Protein Conformation; Protein Denaturation; Structure-Activity Relationship; Temperature; Thiocyanates; Urea | 1993 |
Stabilization of a protein by guanidinium chloride.
Topics: Enzyme Stability; Guanidine; Guanidines; Kinetics; Protein Conformation; Protein Denaturation; Ribonuclease T1; Ribonuclease, Pancreatic; Sodium Chloride; Thermodynamics; Urea | 1993 |
Effect of some products of protein catabolism on the endothelium-dependent and -independent relaxation of rabbit thoracic aorta rings.
Topics: Animals; Aorta, Thoracic; Creatinine; Endothelium, Vascular; Guanidine; Guanidines; In Vitro Techniques; Methylguanidine; Nitric Oxide; Proteins; Rabbits; Urea; Uremia; Vasodilation | 1993 |
Localization of "non-extractable" acetylcholinesterase to the vertebrate neuromuscular junction.
Topics: Acetylcholinesterase; Animals; Buffers; Collagenases; Fluorescent Antibody Technique; Guanidine; Guanidines; Heparin; Male; Muscles; Neuromuscular Junction; Osmolar Concentration; Quail; Sodium Chloride; Solubility; Urea | 1993 |
Structure of the herpes simplex virus capsid. Molecular composition of the pentons and the triplexes.
Topics: Capsid; Capsid Proteins; Guanidine; Guanidines; Image Processing, Computer-Assisted; Microscopy, Electron; Simplexvirus; Urea | 1993 |
Urea-generated free rotating water molecules are active in the protein unfolding process.
Topics: Chymotrypsinogen; Guanidine; Guanidines; Hydrogen Bonding; Motion; Protein Denaturation; Serum Albumin, Bovine; Spectrophotometry, Ultraviolet; Urea; Water | 1993 |
Guanidine hydrochloride stabilization of a partially unfolded intermediate during the reversible denaturation of protein disulfide isomerase.
Topics: Animals; Calorimetry; Cattle; Enzyme Stability; Guanidine; Guanidines; Isoenzymes; Isomerases; Kinetics; Liver; Protein Denaturation; Protein Disulfide-Isomerases; Protein Folding; Urea | 1993 |
The energetics and cooperativity of protein folding: a simple experimental analysis based upon the solvation of internal residues.
Topics: Algorithms; Amino Acid Sequence; Calorimetry; Geobacillus stearothermophilus; Guanidine; Guanidines; Kinetics; Mathematics; Micrococcal Nuclease; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphoglycerate Kinase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Software; Staphylococcus aureus; Thermodynamics; Tryptophan; Urea | 1993 |
Engineered disulfide bonds as probes of the folding pathway of barnase: increasing the stability of proteins against the rate of denaturation.
Topics: Bacillus; Bacterial Proteins; Calorimetry; Cloning, Molecular; Disulfides; Escherichia coli; Guanidine; Guanidines; Kinetics; Mathematics; Models, Molecular; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Ribonucleases; Urea | 1993 |
Refolding and purification of Ras proteins.
Topics: Escherichia coli; Guanidine; Guanidines; Humans; Inclusion Bodies; Magnetic Resonance Spectroscopy; Peptide Fragments; Protein Folding; Proto-Oncogene Proteins p21(ras); Recombinant Proteins; Solubility; Urea | 1995 |
Folding pathway of Escherichia coli ribonuclease HI: a circular dichroism, fluorescence, and NMR study.
Topics: Amino Acid Sequence; Circular Dichroism; Enzyme Stability; Escherichia coli; Guanidine; Guanidines; Hydrogen; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Molecular Structure; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Ribonuclease H; Spectrometry, Fluorescence; Thermodynamics; Urea | 1995 |
Reversible dissociation and unfolding of dimeric creatine kinase isoenzyme MM in guanidine hydrochloride and urea.
Topics: Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Creatine Kinase; Fluorescent Dyes; Guanidine; Guanidines; Isoenzymes; Muscles; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Urea | 1995 |
Probes of energy transduction in enzyme catalysis.
Topics: Acylation; Catalysis; Chymotrypsin; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Protein Binding; Protein Folding; Spectrophotometry; Thermodynamics; Titrimetry; Urea | 1995 |
How to measure and predict the molar absorption coefficient of a protein.
Topics: 1-Propanol; Chemical Phenomena; Chemistry, Physical; Cystine; Guanidine; Guanidines; Proteins; Solvents; Spectrophotometry, Ultraviolet; Tryptophan; Tyrosine; Urea; Water | 1995 |
Chaotropic solvents increase the critical micellar concentrations of detergents.
Topics: Detergents; Formamides; Guanidine; Guanidines; Micelles; Ultrafiltration; Urea | 1995 |
Catalytic ability and stability of two recombinant mutants of D-amino acid transaminase involved in coenzyme binding.
Topics: Alanine; Base Sequence; beta-Alanine; Binding Sites; Catalysis; D-Alanine Transaminase; Enzyme Inhibitors; Enzyme Stability; Guanidine; Guanidines; Hot Temperature; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Denaturation; Pyridoxal Phosphate; Recombinant Proteins; Spectrophotometry; Structure-Activity Relationship; Substrate Specificity; Sulfhydryl Compounds; Transaminases; Urea | 1995 |
Mechanism of laminin chain assembly into a triple-stranded coiled-coil structure.
Topics: Amino Acid Sequence; Animals; Disulfides; Drug Stability; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Laminin; Macromolecular Substances; Mice; Models, Structural; Molecular Sequence Data; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Thermodynamics; Urea | 1996 |
The role of the dodecamer subunit in the dissociation and reassembly of the hexagonal bilayer structure of Lumbricus terrestris hemoglobin.
Topics: Animals; Annelida; Calcium; Guanidine; Guanidines; Macromolecular Substances; Methemoglobin; Microscopy, Electron, Scanning Transmission; Oxyhemoglobins; Protein Binding; Protein Denaturation; Urea | 1996 |
Fluorescence studies of the effect of pH, guanidine hydrochloride and urea on equinatoxin II conformation.
Topics: Anilino Naphthalenesulfonates; Animals; Cnidarian Venoms; Cytotoxins; Fluorescent Dyes; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Protein Denaturation; Sea Anemones; Spectrometry, Fluorescence; Urea | 1996 |
The detection of kinetic intermediates during the unfolding of lipoxygenase-1 by urea or guanidine hydrochloride.
Topics: Circular Dichroism; Glycine max; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Lipoxygenase; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Urea | 1996 |
Characterization of the denaturation and renaturation of human plasma vitronectin. I. Biophysical characterization of protein unfolding and multimerization.
Topics: Chromatography, High Pressure Liquid; Circular Dichroism; Guanidine; Guanidines; Humans; Kinetics; Macromolecular Substances; Models, Theoretical; Molecular Weight; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Ultracentrifugation; Urea; Vitronectin | 1996 |
Activation of chicken liver dihydrofolate reductase by urea and guanidine hydrochloride is accompanied by conformational change at the active site.
Topics: Amino Acid Sequence; Animals; Binding Sites; Chickens; Enzyme Activation; Fluorescence; Fluorescent Dyes; Guanidine; Guanidines; Liver; Mice; Molecular Sequence Data; Naphthalenesulfonates; Peptide Fragments; Protein Conformation; Protein Denaturation; Solutions; Tetrahydrofolate Dehydrogenase; Trypsin; Urea | 1996 |
The enthalpy of transfer of unfolded proteins into solutions of urea and guanidinium chloride.
Topics: Binding Sites; Guanidine; Guanidines; Models, Chemical; Protein Conformation; Protein Denaturation; Protein Folding; Solutions; Thermodynamics; Urea | 1996 |
Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding.
Topics: Binding Sites; Chromatography, Gel; Circular Dichroism; Fluorescence; Guanidine; Guanidines; Lacticaseibacillus casei; Models, Chemical; Models, Molecular; Molecular Probes; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Thymidylate Synthase; Tryptophan; Urea | 1996 |
Guanidine hydrochloride unfolding of peptide helices: separation of denaturant and salt effects.
Topics: Amino Acid Sequence; Circular Dichroism; Guanidine; Guanidines; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Salts; Sodium Chloride; Thermodynamics; Urea | 1996 |
Quantitative solubilization and analysis of insoluble paired helical filaments from Alzheimer disease.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Alkalies; Alzheimer Disease; Cell Fractionation; Cross-Linking Reagents; Densitometry; Endopeptidases; Formates; Glycation End Products, Advanced; Guanidine; Guanidines; Hemostatics; Humans; Immunoblotting; Microscopy, Electron; Middle Aged; Neurofilament Proteins; Phosphorylation; Protein Denaturation; Sodium Dodecyl Sulfate; Solubility; Urea | 1996 |
Thermodynamics of denaturation of alpha-chymotrypsinogen A in aqueous urea and alkylurea solutions.
Topics: Calorimetry, Differential Scanning; Chromatography, Gel; Chymotrypsinogen; Enzyme Stability; Guanidine; Guanidines; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Sodium Chloride; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Temperature; Thermodynamics; Urea | 1995 |
Evaluation of solubilizing methods of the egg envelope of the fish, Oryzias latipes, and partial determination of amino acid sequence of its subunit protein, ZI-3.
Topics: Amino Acid Sequence; Amino Acids; Animals; Chromatography, High Pressure Liquid; Egg Proteins; Electrophoresis, Polyacrylamide Gel; Female; Guanidine; Guanidines; Molecular Sequence Data; Oryzias; Sodium Dodecyl Sulfate; Solubility; Urea | 1996 |
Preferential interaction of denaturants with rice bran lipase.
Topics: Enzyme Stability; Guanidine; Guanidines; Lipase; Oryza; Protein Denaturation; Thiocyanates; Urea | 1996 |
Protein stability: urea-induced versus guanidine-induced unfolding of metmyoglobin.
Topics: Animals; Guanidine; Guanidines; Horses; Metmyoglobin; Myocardium; Protein Denaturation; Protein Folding; Spectrophotometry; Thermodynamics; Urea | 1996 |
Self-catalyzed insertion of proteins into phospholipid membranes.
Topics: Catalysis; Guanidine; Guanidines; Hydrogen Bonding; Membrane Lipids; Membrane Potentials; Membrane Proteins; Membranes, Artificial; Mitochondria; Osmolar Concentration; Phospholipids; Porins; Protein Denaturation; Sarcosine; Urea; Voltage-Dependent Anion Channels | 1996 |
Studies on activation and inhibition of cathepsin B from buffalo liver.
Topics: Animals; Benzoylarginine-2-Naphthylamide; Buffaloes; Calcium; Cathepsin B; Copper; Edetic Acid; Enzyme Activation; Enzyme Stability; Guanidine; Guanidines; Liver; Protein Denaturation; Sulfhydryl Compounds; Sulfhydryl Reagents; Temperature; Urea | 1996 |
Purification and characterization of NADH oxidase from the archaea Sulfolobus acidocaldarius and Sulfolobus solfataricus.
Topics: Amino Acid Sequence; Enzyme Stability; Guanidine; Guanidines; Hot Temperature; Kinetics; Molecular Sequence Data; Molecular Weight; Multienzyme Complexes; NADH, NADPH Oxidoreductases; Protein Denaturation; Solvents; Spectrophotometry; Sulfolobus; Sulfolobus acidocaldarius; Urea | 1996 |
Denaturants can accelerate folding rates in a class of globular proteins.
Topics: Aprotinin; Cysteine; Disulfides; Guanidine; Guanidines; Kinetics; Models, Molecular; Monte Carlo Method; Protein Denaturation; Protein Folding; Ribonuclease T1; Thermodynamics; Urea | 1996 |
Urea-induced equilibrium unfolding of single tryptophan mutants of yeast phosphoglycerate kinase: evidence for a stable intermediate.
Topics: Circular Dichroism; Guanidine; Guanidines; Kinetics; Phosphoglycerate Kinase; Point Mutation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1996 |
A lysine 73-->histidine variant of yeast iso-1-cytochrome c: evidence for a native-like intermediate in the unfolding pathway and implications for m value effects.
Topics: Circular Dichroism; Cytochrome c Group; Cytochromes c; Fluorescence; Fungal Proteins; Guanidine; Guanidines; Hydrogen-Ion Concentration; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Thermodynamics; Tryptophan; Urea | 1997 |
Stability of D-amino acid oxidase: denaturation by guanidine hydrochloride and urea.
Topics: Animals; D-Amino-Acid Oxidase; Dimerization; Enzyme Stability; Guanidine; Guanidines; In Vitro Techniques; Protein Denaturation; Protein Folding; Swine; Thermodynamics; Urea | 1996 |
De novo design of heterotrimeric coiled coils.
Topics: Amino Acid Sequence; Binding Sites; Circular Dichroism; Crystallography, X-Ray; Drug Design; Guanidine; Guanidines; Molecular Sequence Data; Peptides; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Temperature; Ultracentrifugation; Urea | 1996 |
All-or-none solvent-induced transitions between native, molten globule and unfolded states in globular proteins.
Topics: Guanidine; Guanidines; Models, Chemical; Molecular Weight; Protein Conformation; Protein Denaturation; Proteins; Solvents; Thermodynamics; Urea | 1996 |
Purification and properties of the Mycobacterium smegmatis mc(2)155 beta-lactamase.
Topics: Bacterial Proteins; beta-Lactamases; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Mycobacterium; Protein Denaturation; Sequence Homology, Amino Acid; Urea | 1997 |
Calorimetrically-derived parameters for protein interactions with urea and guanidine-HCl are not consistent with denaturant m values.
Topics: Calorimetry; Guanidine; Guanidines; Protein Denaturation; Proteins; Ribonucleases; Thermodynamics; Urea | 1997 |
Reactivation of C1-inhibitor polymers by denaturation and gel-filtration chromatography.
Topics: Chromatography, Gel; Complement C1 Inactivator Proteins; Detergents; Dimerization; Guanidine; Guanidines; Humans; In Vitro Techniques; Polymers; Protein Conformation; Protein Denaturation; Protein Folding; Urea | 1997 |
The domains in gammaB-crystallin: identical fold-different stabilities.
Topics: Amino Acid Sequence; Circular Dichroism; Crystallins; Dimerization; Guanidine; Guanidines; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Urea | 1997 |
Mutational analysis of hydrophobic domain interactions in gamma B-crystallin from bovine eye lens.
Topics: Animals; Cattle; Circular Dichroism; Crystallins; DNA Mutational Analysis; gamma-Crystallins; Guanidine; Guanidines; Lens, Crystalline; Models, Molecular; Mutagenesis, Site-Directed; Peptide Fragments; Phenylalanine; Protein Conformation; Protein Denaturation; Recombinant Proteins; Urea | 1997 |
A prediction of DPP IV/CD26 domain structure from a physico-chemical investigation of dipeptidyl peptidase IV (CD26) from human seminal plasma.
Topics: Amino Acid Sequence; Dipeptidyl Peptidase 4; Glycosylation; Guanidine; Guanidines; Humans; Molecular Sequence Data; Protein Folding; Protein Structure, Secondary; Semen; Urea | 1997 |
The effect of denaturants on protein structure.
Topics: Crystallography, X-Ray; Guanidine; Guanidines; Protein Conformation; Protein Denaturation; Ribonuclease, Pancreatic; Tetrahydrofolate Dehydrogenase; Urea | 1997 |
Unfolding of dimeric creatine kinase in urea and guanidine hydrochloride as measured using small angle X-ray scattering with synchrotron radiation.
Topics: Animals; Creatine Kinase; Dimerization; Fluorescence; Guanidine; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Rabbits; Scattering, Radiation; Urea; X-Rays | 1997 |
On the rôle of human salivary micelle-like globules in bacterial agglutination.
Topics: Adult; Aged; Agglutination; Calcium; Coloring Agents; Diphosphates; Female; Guanidine; Humans; Male; Micelles; Microscopy, Electron; Middle Aged; Mouth; Parotid Gland; Saliva; Salivary Proteins and Peptides; Spectrophotometry, Ultraviolet; Static Electricity; Streptococcus mutans; Streptococcus sanguis; Surface Properties; Time Factors; Urea | 1997 |
Organophosphorus hydrolase is a remarkably stable enzyme that unfolds through a homodimeric intermediate.
Topics: Aryldialkylphosphatase; Calorimetry; Chromatography, Gel; Computer Graphics; Computer Simulation; Dimerization; Escherichia coli; Esterases; Guanidine; Kinetics; Models, Chemical; Models, Molecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Thermodynamics; Urea | 1997 |
Altered ligand binding properties and enhanced stability of a constitutively active estrogen receptor: evidence that an open pocket conformation is required for ligand interaction.
Topics: Binding Sites; Estradiol; Guanidine; Humans; Ligands; Models, Chemical; Point Mutation; Protein Conformation; Protein Denaturation; Receptors, Estrogen; Recombinant Proteins; Tyrosine; Urea | 1997 |
Time-resolved protein phosphorescence in the stopped-flow: denaturation of horse liver alcohol dehydrogenase by urea and guanidine hydrochloride.
Topics: Alcohol Dehydrogenase; Animals; Circular Dichroism; Guanidine; Horses; Kinetics; Liver; Luminescent Measurements; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Sodium Chloride; Spectrometry, Fluorescence; Spectrum Analysis; Tryptophan; Urea | 1997 |
Inactivation and conformational changes of yeast invertase during unfolding in urea and guanidinium chloride solutions.
Topics: beta-Fructofuranosidase; Glycoside Hydrolases; Guanidine; Protein Conformation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Urea | 1998 |
Anion binding to the ubiquitin molecule.
Topics: Animals; Anions; Calorimetry, Differential Scanning; Cattle; Chlorides; Circular Dichroism; Guanidine; Humans; Hydrogen-Ion Concentration; Osmolar Concentration; Protein Denaturation; Spectrometry, Fluorescence; Thermodynamics; Ubiquitins; Urea | 1998 |
Equilibrium unfolding of a small bacterial cytochrome, cytochrome c551 from Pseudomonas aeruginosa.
Topics: Acids; Animals; Bacterial Proteins; Circular Dichroism; Cytochrome c Group; Enzyme Stability; Guanidine; Horses; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Protein Denaturation; Protein Engineering; Protein Folding; Protein Structure, Secondary; Pseudomonas aeruginosa; Salts; Spectrometry, Fluorescence; Thermodynamics; Urea | 1998 |
Cooperativity in protein folding: from lattice models with sidechains to real proteins.
Topics: Fibronectins; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Peptides; Plant Proteins; Protein Denaturation; Protein Folding; Proteins; Ribonucleases; Temperature; Thermodynamics; Trifluoroethanol; Urea | 1998 |
A monomeric mutant of Clostridium symbiosum glutamate dehydrogenase: comparison with a structured monomeric intermediate obtained during refolding.
Topics: Anilino Naphthalenesulfonates; Binding Sites; Circular Dichroism; Clostridium; Computer Simulation; Escherichia coli; Fluorescence; Glutamate Dehydrogenase; Guanidine; Molecular Weight; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Ultracentrifugation; Urea | 1998 |
Stabilization of the glucan-binding lectin of Streptococcus sobrinus by specific ligand.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Cell Wall; Cetylpyridinium; Dental Plaque; Dentifrices; Detergents; Endopeptidases; Glucans; Guanidine; Humans; Lectins; Ligands; Molecular Weight; Muramidase; Octoxynol; Sodium Dodecyl Sulfate; Streptococcus sobrinus; Sucrose; Urea | 1998 |
Atomic mutations in annexin V--thermodynamic studies of isomorphous protein variants.
Topics: Annexin A5; Circular Dichroism; Genetic Variation; Guanidine; Humans; In Vitro Techniques; Methionine; Models, Molecular; Point Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Solubility; Temperature; Thermodynamics; Urea | 1998 |
Stability of polyomavirus major capsid protein VP1 under denaturants guanidine hydrochloride and urea.
Topics: Capsid; Capsid Proteins; Circular Dichroism; Guanidine; Polyomavirus; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Urea | 1998 |
Folding kinetics of a fluorescent variant of monomeric lambda repressor.
Topics: Amino Acid Substitution; Bacteriophage lambda; Circular Dichroism; DNA-Binding Proteins; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Repressor Proteins; Spectrometry, Fluorescence; Tryptophan; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 1998 |
Enhancement of transcriptional activity of T7 RNA polymerase by guanidine hydrochloride.
Topics: Bacteriophage T7; Circular Dichroism; DNA-Directed RNA Polymerases; Dose-Response Relationship, Drug; Guanidine; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Transcription, Genetic; Trypsin; Urea; Viral Proteins | 1998 |
Incorrect folding of steroidogenic acute regulatory protein (StAR) in congenital lipoid adrenal hyperplasia.
Topics: Adrenal Hyperplasia, Congenital; Circular Dichroism; Escherichia coli; Guanidine; Hot Temperature; Humans; Mutagenesis, Site-Directed; Phosphoproteins; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectroscopy, Fourier Transform Infrared; Urea | 1998 |
Control of the association state of tetrameric glucose-fructose oxidoreductase from Zymomonas mobilis as the rationale for stabilization of the enzyme in biochemical reactors.
Topics: Enzyme Repression; Enzyme Stability; Guanidine; Oxidoreductases; Protein Conformation; Protein Denaturation; Protein Folding; Time Factors; Urea; Zymomonas | 1998 |
The influence of chaotropic reagents on neuronal nitric oxide synthase and its flavoprotein module. Urea and guanidine hydrochloride stimulate NADPH-cytochrome c reductase activity of both proteins.
Topics: Base Sequence; Catalysis; DNA Primers; Enzyme Activation; Flavin Mononucleotide; Flavin-Adenine Dinucleotide; Guanidine; Indicators and Reagents; NADPH-Ferrihemoprotein Reductase; Nitric Oxide Synthase; Nitric Oxide Synthase Type I; Spectrometry, Fluorescence; Urea | 1997 |
Folding kinetics of villin 14T, a protein domain with a central beta-sheet and two hydrophobic cores.
Topics: Animals; Carrier Proteins; Chickens; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Microfilament Proteins; Peptide Fragments; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thermodynamics; Urea | 1998 |
Thermodynamic and kinetic characterization of calf lens gammaF-crystallin.
Topics: Animals; Cattle; Circular Dichroism; Crystallins; Guanidine; Kinetics; Lens, Crystalline; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1998 |
Reversible inhibition by pyridoxal 5'-phosphate and irreversible inactivation by urea and guanidine hydrochloride of thymidylate synthase from Lactobacillus leichmannii.
Topics: Dimerization; Enzyme Inhibitors; Guanidine; Lactobacillus; Protein Conformation; Pyridoxal Phosphate; Thymidylate Synthase; Urea | 1998 |
Unfolding of Plasmodium falciparum triosephosphate isomerase in urea and guanidinium chloride: evidence for a novel disulfide exchange reaction in a covalently cross-linked mutant.
Topics: Animals; Chromatography, Gel; Circular Dichroism; Cross-Linking Reagents; Cysteine; Disulfides; Guanidine; Models, Molecular; Mutagenesis, Site-Directed; Plasmodium falciparum; Protein Conformation; Protein Denaturation; Recombinant Proteins; Spectrometry, Fluorescence; Triose-Phosphate Isomerase; Tryptophan; Tyrosine; Urea | 1999 |
Studies of the denaturation patterns of bovine alpha-crystallin using an ionic denaturant, guanidine hydrochloride and a non-ionic denaturant, urea.
Topics: Animals; Cattle; Chromatography, Liquid; Crystallins; Electrophoresis, Polyacrylamide Gel; Guanidine; Isotonic Solutions; Microscopy, Electron; Protein Denaturation; Protein Structure, Secondary; Urea | 1998 |
Protein stability: functional dependence of denaturational Gibbs energy on urea concentration.
Topics: Animals; Cattle; Chickens; Cytochrome c Group; Egg White; Guanidine; Horses; Models, Chemical; Muramidase; Protein Conformation; Protein Denaturation; Proteins; Ribonuclease, Pancreatic; Spectrophotometry, Ultraviolet; Thermodynamics; Urea | 1999 |
Tolerance of Arc repressor to multiple-alanine substitutions.
Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Circular Dichroism; Dimerization; DNA-Binding Proteins; Guanidine; Hot Temperature; Kinetics; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Repressor Proteins; Thermodynamics; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 1999 |
Fluorescence and quenching comparative studies of halophilic and bovine glutamate dehydrogenase.
Topics: Acrylamide; Animals; Cattle; Fluorescence; Glutamate Dehydrogenase (NADP+); Guanidine; Haloferax mediterranei; Potassium Chloride; Protein Denaturation; Sodium Chloride; Spectrometry, Fluorescence; Urea | 1998 |
Equilibrium and transient intermediates in folding of human macrophage migration inhibitory factor.
Topics: Circular Dichroism; Escherichia coli; Guanidine; Humans; Hydrogen-Ion Concentration; Kinetics; Macrophage Migration-Inhibitory Factors; Protein Denaturation; Protein Folding; Recombinant Proteins; Urea | 1999 |
Catalase HPII from Escherichia coli exhibits enhanced resistance to denaturation.
Topics: Catalase; Circular Dichroism; Enzyme Activation; Enzyme Stability; Escherichia coli; Guanidine; Hot Temperature; Models, Molecular; Mutagenesis, Site-Directed; Protein Denaturation; Sodium Dodecyl Sulfate; Urea | 1999 |
Nativelike structure and stability in a truncation mutant of a protein minidomain: the peripheral subunit-binding domain.
Topics: Amino Acid Sequence; Enzyme Stability; Guanidine; Hot Temperature; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Magnetic Resonance, Biomolecular; Peptide Fragments; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Pyruvate Dehydrogenase Complex; Thermodynamics; Urea | 1999 |
Folding-unfolding equilibrium and kinetics of equine beta-lactoglobulin: equivalence between the equilibrium molten globule state and a burst-phase folding intermediate.
Topics: Animals; Cattle; Circular Dichroism; Guanidine; Horses; Kinetics; Lactoglobulins; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Urea | 1999 |
WW: An isolated three-stranded antiparallel beta-sheet domain that unfolds and refolds reversibly; evidence for a structured hydrophobic cluster in urea and GdnHCl and a disordered thermal unfolded state.
Topics: Adaptor Proteins, Signal Transducing; Biochemical Phenomena; Carrier Proteins; Circular Dichroism; Dose-Response Relationship, Drug; Guanidine; Humans; Kinetics; Magnetic Resonance Spectroscopy; Models, Statistical; Phosphoproteins; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Temperature; Thermodynamics; Transcription Factors; Urea; YAP-Signaling Proteins | 1999 |
A pathway for conformational diversity in proteins mediated by intramolecular chaperones.
Topics: Amino Acid Sequence; Bacillus subtilis; Binding Sites; Enzyme Precursors; Escherichia coli; Guanidine; Kinetics; Models, Molecular; Molecular Chaperones; Molecular Sequence Data; Peptide Fragments; Point Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Sequence Alignment; Subtilisins; Temperature; Urea | 1999 |
Renaturation of Escherichia coli-derived recombinant human macrophage colony-stimulating factor.
Topics: Dimerization; Escherichia coli; Guanidine; Humans; In Vitro Techniques; Kinetics; Macrophage Colony-Stimulating Factor; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Sulfhydryl Compounds; Temperature; Urea | 1999 |
Colicin E1 forms a dimer after urea-induced unfolding.
Topics: Amino Acid Sequence; Amino Acid Substitution; Chromatography, High Pressure Liquid; Circular Dichroism; Colicins; Dimerization; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Models, Molecular; Molecular Sequence Data; Naphthalenesulfonates; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
Guanidine hydrochloride exerts dual effects on the tryptophan synthase alpha 2 beta 2 complex as a cation activator and as a modulator of the active site conformation.
Topics: Binding Sites; Cations, Monovalent; Chromatography, Gel; Crystallography, X-Ray; Dose-Response Relationship, Drug; Enzyme Activation; Guanidine; Macromolecular Substances; Models, Chemical; Protein Conformation; Serine; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan Synthase; Urea | 1999 |
Different equilibrium stability behavior of ScFv fragments: identification, classification, and improvement by protein engineering.
Topics: Chromatography, Gel; Endopeptidases; Genetic Vectors; Guanidine; Humans; Hydrolysis; Immunoglobulin Fragments; Immunoglobulin Variable Region; Protein Denaturation; Protein Engineering; Protein Folding; Protein Structure, Tertiary; Recombinant Proteins; Temperature; Thermolysin; Urea | 1999 |
Partially folded intermediates during trypsinogen denaturation.
Topics: Animals; Cattle; Diuretics, Osmotic; Guanidine; Parasympathomimetics; Protein Denaturation; Protein Folding; Trypsinogen; Urea | 1999 |
Analysis of the extent of unfolding of denatured insulin-like growth factor.
Topics: Guanidine; Guanidines; Protein Denaturation; Somatomedins; Thiocyanates; Urea | 1999 |
The role of intermolecular interactions: studies on model systems for bacterial biofilms.
Topics: Acrylic Resins; Alginates; Biofilms; Electrolytes; Glucuronic Acid; Guanidine; Hexuronic Acids; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Methylurea Compounds; Models, Biological; Models, Chemical; Osmolar Concentration; Polymers; Pseudomonas aeruginosa; Rheology; Solutions; Static Electricity; Urea; Viscosity | 1999 |
Pollen-stigma adhesion in Arabidopsis: a species-specific interaction mediated by lipophilic molecules in the pollen exine.
Topics: Arabidopsis; Cell Adhesion; Guanidine; Molecular Biology; Mutation; Plant Proteins; Pollen; Reproduction; Species Specificity; Urea | 1999 |
Probing the folding and unfolding of wild-type and mutant forms of bacteriorhodopsin in micellar solutions: evaluation of reversible unfolding conditions.
Topics: Amino Acid Substitution; Bacteriorhodopsins; Cholic Acids; Detergents; Dimyristoylphosphatidylcholine; Guanidine; Halobacterium salinarum; Hydrogen-Ion Concentration; Micelles; Point Mutation; Protein Denaturation; Protein Folding; Recombinant Fusion Proteins; Sodium Dodecyl Sulfate; Solutions; Spectrophotometry, Ultraviolet; Temperature; Urea | 1999 |
Rapid screening of elution conditions prior to immunopurification of proteins.
Topics: Antibodies, Monoclonal; Chlorella; Chromatography, Affinity; Deoxyribonucleases, Type II Site-Specific; Guanidine; Immunosorbents; Phycodnaviridae; Sepharose; Urea | 1999 |
Fluorescence and circular dichroism spectroscopic studies on bovine lactoperoxidase.
Topics: Acrylamide; Animals; Cattle; Cesium; Circular Dichroism; Fluorescent Dyes; Guanidine; Iodine; Lactoperoxidase; Milk; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
The role of position a in determining the stability and oligomerization state of alpha-helical coiled coils: 20 amino acid stability coefficients in the hydrophobic core of proteins.
Topics: Amino Acid Sequence; Circular Dichroism; Disulfides; Drug Stability; Guanidine; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Structure, Secondary; Proteins; Regression Analysis; Thermodynamics; Urea | 1999 |
Thermodynamic features of the chemical and thermal denaturations of human serum albumin.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Hot Temperature; Humans; Protein Denaturation; Protein Folding; Serum Albumin; Spectrometry, Fluorescence; Thermodynamics; Urea | 1999 |
Aggregation and fibrillization of the recombinant human prion protein huPrP90-231.
Topics: Chromatography, Gel; Circular Dichroism; Endopeptidase K; Guanidine; Humans; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Peptide Fragments; Plasmids; Prions; Protein Conformation; PrPC Proteins; PrPSc Proteins; Recombinant Proteins; Sodium Chloride; Urea | 2000 |
Molecular basis for the polymerization of octopus lens S-crystallin.
Topics: Animals; Biophysical Phenomena; Biophysics; Biopolymers; Crystallins; Glutathione Transferase; Guanidine; Hydrogen-Ion Concentration; In Vitro Techniques; Microscopy, Electron; Models, Molecular; Octopodiformes; Protein Denaturation; Protein Structure, Quaternary; Protein Structure, Tertiary; Sodium Chloride; Spectrometry, Fluorescence; Static Electricity; Temperature; Urea | 2000 |
Hierarchical unfolding of the alpha-lactalbumin molten globule: presence of a compact intermediate without a unique tertiary fold.
Topics: Amino Acid Substitution; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Disulfides; Dose-Response Relationship, Drug; Guanidine; Humans; Isomerism; Lactalbumin; Models, Molecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Urea | 2000 |
Unfolding and disassembly of the chaperonin GroEL occurs via a tetradecameric intermediate with a folded equatorial domain.
Topics: Amino Acid Sequence; Chaperonin 60; Chromatography, Gel; Deuterium; Deuterium Oxide; Escherichia coli; Guanidine; Kinetics; Light; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Molecular Weight; Pepsin A; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Tertiary; Scattering, Radiation; Thermodynamics; Urea | 2000 |
Protease inhibitors: synthesis and QSAR study of novel classes of nonbasic thrombin inhibitors incorporating sulfonylguanidine and O-methylsulfonylisourea moieties at P1.
Topics: Computer Simulation; Drug Design; Guanidine; Humans; Regression Analysis; Serine Proteinase Inhibitors; Structure-Activity Relationship; Thrombin; Trypsin Inhibitors; Urea | 2000 |
Extremely high thermal stability of streptavidin and avidin upon biotin binding.
Topics: Avidin; Biotin; Calorimetry, Differential Scanning; Drug Stability; Guanidine; Hot Temperature; Ligands; Protein Binding; Protein Denaturation; Protein Engineering; Protein Structure, Quaternary; Streptavidin; Thermodynamics; Urea | 1999 |
The collagen-like peptide (GER)15GPCCG forms pH-dependent covalently linked triple helical trimers.
Topics: Biopolymers; Carrier Proteins; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Proteins; Static Electricity; Urea | 2000 |
The structure of denatured bovine pancreatic trypsin inhibitor (BPTI).
Topics: Animals; Aprotinin; Cattle; Disulfides; Dose-Response Relationship, Drug; Guanidine; Guanidines; Protein Conformation; Protein Denaturation; Sequence Analysis, Protein; Thiocyanates; Urea | 2000 |
The ecto-5'-nucleotidase subunits in dimers are not linked by disulfide bridges but by non-covalent bonds.
Topics: 5'-Nucleotidase; Animals; Cattle; Centrifugation, Density Gradient; Chromatography, High Pressure Liquid; Dimerization; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidine; Male; Protein Structure, Quaternary; Sulfhydryl Compounds; Urea | 2000 |
Ion-induced conformational and stability changes in Nereis sarcoplasmic calcium binding protein: evidence that the APO state is a molten globule.
Topics: Animals; Annelida; Calcium; Calcium-Binding Proteins; Chromatography, Gel; Circular Dichroism; Dose-Response Relationship, Drug; Fluorescent Dyes; Guanidine; Hydrogen-Ion Concentration; Ions; Magnetic Resonance Spectroscopy; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Sarcoplasmic Reticulum; Spectrometry, Fluorescence; Temperature; Thermodynamics; Urea | 2000 |
Fluorescence energy transfer indicates similar transient and equilibrium intermediates in staphylococcal nuclease folding.
Topics: Amino Acid Substitution; Anilino Naphthalenesulfonates; Anions; Circular Dichroism; Cysteine; Energy Transfer; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Models, Molecular; Pliability; Proline; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Thermodynamics; Titrimetry; Tryptophan; Urea | 2000 |
The thermodynamic stability of the proteins of the ccd plasmid addiction system.
Topics: Bacterial Proteins; Bacterial Toxins; Calorimetry, Differential Scanning; Circular Dichroism; Dimerization; DNA; DNA-Binding Proteins; Escherichia coli; Fluorescence; Genes, Bacterial; Guanidine; Hydrogen-Ion Concentration; Oligodeoxyribonucleotides; Operator Regions, Genetic; Plasmids; Protein Denaturation; Protein Folding; Substrate Specificity; Temperature; Thermodynamics; Urea | 2000 |
Comparison of inactivation and unfolding of methanol dehydrogenase during denaturation in guanidine hydrochloride and urea.
Topics: Alcohol Oxidoreductases; Binding Sites; Calcium; Dose-Response Relationship, Drug; Guanidine; Kinetics; PQQ Cofactor; Protein Conformation; Protein Denaturation; Protein Folding; Quinolones; Quinones; Spectrometry, Fluorescence; Urea | 2000 |
Identification and characterization of an equilibrium intermediate in the unfolding pathway of an all beta-barrel protein.
Topics: Anilino Naphthalenesulfonates; Chromatography; Densitometry; Deuterium; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Fibroblast Growth Factor 1; Fibroblast Growth Factor 2; Fluorescent Dyes; Guanidine; Heparin; Humans; Hydrogen; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Time Factors; Urea | 2000 |
Structural and functional consequences of removal of the interdomain disulfide bridge from the isolated C-lobe of ovotransferrin.
Topics: Conalbumin; Crystallography, X-Ray; Cysteine; Diphosphates; Disulfides; Guanidine; Iron; Kinetics; Models, Chemical; Peptides; Protein Conformation; Urea | 2000 |
The mitotic apparatus. Physical chemical characterization of the 22S protein component and its subunits.
Topics: Acids; Alkalies; Animals; Glycols; Guanidine; Hydrogen-Ion Concentration; Molecular Weight; Ovum; Proteins; Sea Urchins; Spindle Apparatus; Subcellular Fractions; Sulfur; Urea | 1967 |
Differences in stability among the human apolipoprotein E isoforms determined by the amino-terminal domain.
Topics: Apolipoproteins E; Catalysis; Guanidine; Hot Temperature; Humans; Hydrolysis; Molecular Weight; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Isoforms; Protein Structure, Secondary; Protein Structure, Tertiary; Thermodynamics; Thrombin; Urea | 2000 |
Thermodynamic analysis of interactions between denaturants and protein surface exposed on unfolding: interpretation of urea and guanidinium chloride m-values and their correlation with changes in accessible surface area (ASA) using preferential interactio
Topics: Guanidine; Models, Chemical; Protein Denaturation; Proteins; Thermodynamics; Urea | 2000 |
Equilibrium folding of dimeric class mu glutathione transferases involves a stable monomeric intermediate.
Topics: Anilino Naphthalenesulfonates; Animals; Chromatography, Gel; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Dimerization; Enzyme Stability; Glutaral; Glutathione Transferase; Guanidine; Hot Temperature; Isoenzymes; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Rats; Structure-Activity Relationship; Urea | 2000 |
The residual pro-part of cathepsin C fulfills the criteria required for an intramolecular chaperone in folding and stabilizing the human proenzyme.
Topics: Cathepsin C; Chromatography, Gel; Circular Dichroism; Colorimetry; Dithiothreitol; Enzyme Precursors; Enzyme Stability; Guanidine; Humans; Kinetics; Molecular Chaperones; Oxidation-Reduction; Peptide Fragments; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Recombinant Proteins; Reducing Agents; Thermodynamics; Urea | 2000 |
Formic acid dissolves aggregates of an N-terminal huntingtin fragment containing an expanded polyglutamine tract: applying to quantification of protein components of the aggregates.
Topics: Animals; Caustics; COS Cells; Cysteine Endopeptidases; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Exons; Formates; Green Fluorescent Proteins; Guanidine; Hemostatics; Hydrochloric Acid; Immunoblotting; Lactones; Luminescent Proteins; Membranes, Artificial; Multienzyme Complexes; Nerve Tissue Proteins; Nuclear Proteins; Peptides; Proteasome Endopeptidase Complex; Protein Binding; Recombinant Fusion Proteins; Sodium Hydroxide; Time Factors; Transfection; Trinucleotide Repeat Expansion; Urea | 2000 |
Counteracting effects of renal solutes on amyloid fibril formation by immunoglobulin light chains.
Topics: Amyloid; Amyloidosis; Betaine; Chromatography, High Pressure Liquid; Guanidine; Humans; Immunoglobulin Light Chains; Immunoglobulin Variable Region; Kidney; Kinetics; Lymph Nodes; Sorbitol; Thermodynamics; Urea | 2001 |
pH dependence of stability of staphylococcal nuclease: evidence of substantial electrostatic interactions in the denatured state.
Topics: Acids; Binding Sites; Enzyme Stability; Guanidine; Hydrogen-Ion Concentration; Micrococcal Nuclease; Models, Chemical; Potassium Chloride; Potentiometry; Protein Denaturation; Protein Folding; Protons; Spectrometry, Fluorescence; Static Electricity; Temperature; Thermodynamics; Urea | 2000 |
Binding of disease-associated prion protein to plasminogen.
Topics: Animals; Blotting, Western; Guanidine; Humans; Magnetics; Mice; Mice, Inbred C57BL; Mice, Transgenic; Plasminogen; Prion Diseases; Protein Binding; Protein Conformation; PrP 27-30 Protein; PrPSc Proteins; Structure-Activity Relationship; Urea | 2000 |
The structure of denatured alpha-lactalbumin elucidated by the technique of disulfide scrambling: fractionation of conformational isomers of alpha-lactalbumin.
Topics: Acetonitriles; Biochemistry; Calcium; Chromatography, High Pressure Liquid; Disulfides; Dose-Response Relationship, Drug; Guanidine; Guanidines; Kinetics; Lactalbumin; Models, Molecular; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Solvents; Stereoisomerism; Temperature; Thermodynamics; Thiocyanates; Time Factors; Urea | 2001 |
Conformational origin of the aggregation of recombinant human factor VIII.
Topics: 1-Propanol; Body Temperature; Calorimetry, Differential Scanning; Chromatography, Gel; Circular Dichroism; Factor VIII; Guanidine; Hot Temperature; Humans; Light; Protein Conformation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Scattering, Radiation; Solvents; Spectrometry, Fluorescence; Urea | 2001 |
Reversible denaturation of oligomeric human chaperonin 10: denatured state depends on chemical denaturant.
Topics: Chaperonin 10; Circular Dichroism; Citrate (si)-Synthase; Cross-Linking Reagents; Dose-Response Relationship, Drug; Escherichia coli; Guanidine; Hot Temperature; Humans; Mitochondria; Models, Molecular; Protein Binding; Protein Conformation; Protein Denaturation; Recombinant Proteins; Thermodynamics; Tyrosine; Ultraviolet Rays; Urea | 2000 |
Role of nitric oxide in the synthesis of guanidinosuccinic acid, an activator of the N-methyl-D-aspartate receptor.
Topics: Animals; Argininosuccinic Acid; Guanidine; Guanidines; Hepatocytes; In Vitro Techniques; Male; Molsidomine; Nitric Oxide; Nitric Oxide Donors; Rats; Rats, Wistar; Receptors, N-Methyl-D-Aspartate; Succinates; Urea | 2001 |
Structural alterations and inhibition of unisite and multisite ATP hydrolysis in soluble mitochondrial F1 by guanidinium chloride.
Topics: Adenine Nucleotides; Adenosine Triphosphate; Animals; Binding Sites; Catalysis; Cattle; Dose-Response Relationship, Drug; Guanidine; Hydrolysis; Mitochondria, Heart; Phosphorus Radioisotopes; Protein Denaturation; Proton-Translocating ATPases; Solubility; Urea | 2001 |
Probing copper ligands in denatured Pseudomonas aeruginosa azurin: unfolding His117Gly and His46Gly mutants.
Topics: Azurin; Bacterial Proteins; Binding Sites; Circular Dichroism; Copper; Guanidine; Kinetics; Ligands; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Urea | 2001 |
Isolation and spectroscopic characterization of the structural subunits of keyhole limpet hemocyanin.
Topics: Animals; Binding Sites; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Circular Dichroism; Guanidine; Hemocyanins; Mollusca; Protein Conformation; Protein Denaturation; Protein Subunits; Spectrometry, Fluorescence; Temperature; Urea | 2001 |
Structure--function relationships in bovine thymus 20S proteasome: a fluorimetric study.
Topics: Animals; Cattle; Cysteine Endopeptidases; Fluorescence; Fluorometry; Guanidine; Multienzyme Complexes; Proteasome Endopeptidase Complex; Protein Denaturation; Sodium Dodecyl Sulfate; Structure-Activity Relationship; Thymus Gland; Urea | 2001 |
Association and activation of fructose 1,6-bisphosphase during unfolding and refolding: spectroscopic and enzymatic studies.
Topics: Amino Acid Substitution; Circular Dichroism; Enzyme Activation; Fluorescence; Fructose-Bisphosphatase; Guanidine; Magnesium; Mutation; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Urea | 2001 |
Ribonuclease Rs from Rhizopus stolonifer: lowering of optimum temperature in the presence of urea.
Topics: Binding Sites; Catalysis; Fungal Proteins; Guanidine; Guanidines; Kinetics; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Rhizopus; Ribonucleases; Temperature; Thiocyanates; Urea | 2001 |
Probing the binding site of 800-nm bacteriochlorophyll in the membrane-linked LH2 protein of Rhodobacter capsulatus by local unfolding and chemical modification: evidence for the involvement of a betaHis20 residue.
Topics: Bacteriochlorophylls; Binding Sites; Cell Membrane; Circular Dichroism; Diethyl Pyrocarbonate; Enzyme Inhibitors; Guanidine; Histidine; Hydrogen-Ion Concentration; Imidazoles; Light-Harvesting Protein Complexes; Models, Chemical; Photosynthetic Reaction Center Complex Proteins; Protein Binding; Protein Folding; Protein Structure, Tertiary; Rhodobacter capsulatus; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Urea | 2001 |
Snapshots of protein folding. A study on the multiple transition state pathway of cytochrome c(551) from Pseudomonas aeruginosa.
Topics: Acids; Bacterial Proteins; Cytochrome c Group; Fluorescence; Glutamic Acid; Guanidine; Hydrogen Bonding; Kinetics; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Static Electricity; Thermodynamics; Tryptophan; Urea | 2001 |
Oxidative folding of murine prion mPrP(23-231).
Topics: Animals; Circular Dichroism; Disulfides; Guanidine; Light; Mice; Oxidation-Reduction; Peptide Fragments; Prions; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Scattering, Radiation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Urea | 2001 |
The effect of urea and guanidinium chloride on activity of subtilisin Carlsberg.
Topics: Arginine; Binding, Competitive; Enzyme Stability; Guanidine; Kinetics; Optical Rotatory Dispersion; Protein Conformation; Protein Denaturation; Subtilisins; Urea; Viscosity | 1971 |
Is there stereoelectronic control in hydrolysis of cyclic guanidinium ions?
Topics: Guanidine; Hydrolysis; Magnetic Resonance Spectroscopy; Stereoisomerism; Urea | 2001 |
Kinetic study of folding and misfolding of diacylglycerol kinase in model membranes.
Topics: Amino Acid Sequence; Detergents; Diacylglycerol Kinase; Escherichia coli; Glucosides; Guanidine; Kinetics; Lipid Bilayers; Micelles; Models, Chemical; Molecular Sequence Data; Phosphatidylcholines; Protein Denaturation; Protein Folding; Reproducibility of Results; Solutions; Ultracentrifugation; Urea | 2001 |
Refolding kinetics of cytochrome c(551) reveals a mechanistic difference between urea and guanidine.
Topics: Bacterial Proteins; Cytochrome c Group; Guanidine; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Urea | 2001 |
Prevention of rhodanese aggregation by the chaperonin GroEL.
Topics: Chaperonin 60; Guanidine; Indicators and Reagents; Kinetics; Light; Protein Denaturation; Protein Folding; Scattering, Radiation; Spectrophotometry, Ultraviolet; Thiosulfate Sulfurtransferase; Urea | 2000 |
Application of combined reagent solution to the oxidative refolding of recombinant human interleukin 6.
Topics: Chromatography, High Pressure Liquid; Circular Dichroism; Disulfides; Electrophoresis, Polyacrylamide Gel; Guanidine; Humans; Indicators and Reagents; Interleukin-6; Lithium Chloride; Oxidation-Reduction; Peptide Mapping; Protein Denaturation; Protein Folding; Recombinant Proteins; Serine Endopeptidases; Solutions; Urea | 2001 |
An engineered transthyretin monomer that is nonamyloidogenic, unless it is partially denatured.
Topics: Amino Acid Substitution; Amyloid; Binding Sites; Crystallography, X-Ray; Dimerization; Drug Stability; Fluorescent Dyes; Guanidine; Humans; Hydrogen-Ion Concentration; Macromolecular Substances; Models, Molecular; Mutagenesis, Site-Directed; Nuclear Magnetic Resonance, Biomolecular; Prealbumin; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Ultracentrifugation; Urea | 2001 |
Anion shielding of electrostatic repulsions in transthyretin modulates stability and amyloidosis: insight into the chaotrope unfolding dichotomy.
Topics: Amino Acid Substitution; Amyloid; Amyloidosis; Anions; Binding Sites; Cross-Linking Reagents; Drug Stability; Glutaral; Guanidine; Humans; Kinetics; Macromolecular Substances; Models, Molecular; Mutagenesis, Site-Directed; Potassium Chloride; Prealbumin; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Static Electricity; Thyroxine; Urea | 2001 |
Role of the connectivity of secondary structure segments in the folding of alpha(1)-antitrypsin.
Topics: alpha 1-Antitrypsin; Circular Dichroism; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Humans; Hydrogen-Ion Concentration; Models, Molecular; Parasympathomimetics; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Serpins; Spectrometry, Fluorescence; Urea | 2001 |
High pressure refolding of recombinant human growth hormone from insoluble aggregates. Structural transformations, kinetic barriers, and energetics.
Topics: Dose-Response Relationship, Drug; Guanidine; Human Growth Hormone; Humans; Kinetics; Pressure; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Temperature; Thermodynamics; Time Factors; Ultraviolet Rays; Urea | 2001 |
p53 unfolding detected by CD but not by tryptophan fluorescence.
Topics: Circular Dichroism; Guanidine; Humans; Potassium Iodide; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Tumor Suppressor Protein p53; Urea | 2001 |
The use of water structure breakers, urea and guanidium chloride, new mobile phase modifiers in reversed-phase partition high-performance liquid chromatography.
Topics: Anions; Chromatography, High Pressure Liquid; Chromatography, Liquid; Dose-Response Relationship, Drug; Guanidine; Temperature; Time Factors; Urea; Water | 2001 |
Thermodynamics of interactions of urea and guanidinium salts with protein surface: relationship between solute effects on protein processes and changes in water-accessible surface area.
Topics: Animals; Cattle; Dose-Response Relationship, Drug; Guanidine; Guanidines; Models, Theoretical; Osmolar Concentration; Potassium Chloride; Protein Binding; Protein Folding; Protein Structure, Tertiary; Serum Albumin; Thermodynamics; Thiocyanates; Urea; Water | 2001 |
Analysis of equilibrium dissociation and unfolding in denaturants of soybean agglutinin and two of its derivatives.
Topics: Chromatography; Chromatography, Gel; Dimerization; Guanidine; Kinetics; Lectins; Plant Lectins; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Tertiary; Soybean Proteins; Spectrometry, Fluorescence; Thermodynamics; Time Factors; Urea | 2001 |
Multiple unfolding intermediates of human placental alkaline phosphatase in equilibrium urea denaturation.
Topics: Alkaline Phosphatase; Anilino Naphthalenesulfonates; Binding Sites; Circular Dichroism; Dose-Response Relationship, Drug; Enzyme Stability; Fluorescent Dyes; Guanidine; Humans; Kinetics; Models, Molecular; Placenta; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Salts; Sodium Chloride; Spectrometry, Fluorescence; Thermodynamics; Urea | 2001 |
Unique oligomeric intermediates of bovine liver catalase.
Topics: Anilino Naphthalenesulfonates; Animals; Catalase; Cattle; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Dimerization; Dose-Response Relationship, Drug; Fluorescent Dyes; Glutaral; Guanidine; Liver; Protein Folding; Protein Structure, Quaternary; Protein Structure, Tertiary; Sodium Chloride; Spectrometry, Fluorescence; Time Factors; Urea | 2002 |
Does beta-lactoglobulin denaturation occur via an intermediate state?
Topics: Animals; Cattle; Circular Dichroism; Fluorescence Polarization; Guanidine; Hydrogen-Ion Concentration; Kinetics; Lactoglobulins; Models, Chemical; Protein Denaturation; Spectrophotometry, Ultraviolet; Urea | 2002 |
SNARE assembly and disassembly exhibit a pronounced hysteresis.
Topics: Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Endosomes; Guanidine; Kinetics; Macromolecular Substances; Membrane Proteins; Nerve Tissue Proteins; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Qa-SNARE Proteins; R-SNARE Proteins; SNARE Proteins; Synapses; Synaptosomal-Associated Protein 25; Temperature; Thermodynamics; Urea; Vesicular Transport Proteins | 2002 |
Temperature- and denaturant-induced unfolding of two thermophilic esterases.
Topics: Bacillus; Circular Dichroism; Enzyme Stability; Esterases; Guanidine; Protein Conformation; Protein Denaturation; Protein Folding; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Temperature; Urea | 2002 |
Unfolding rates of barstar determined in native and low denaturant conditions indicate the presence of intermediates.
Topics: Bacterial Proteins; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Enzyme Inhibitors; Guanidine; Kinetics; Protein Denaturation; Protein Folding; Solutions; Spectrometry, Fluorescence; Sulfhydryl Reagents; Urea | 2002 |
Stabilization of molten globule state of papain by urea.
Topics: Circular Dichroism; Dose-Response Relationship, Drug; Drug Stability; Guanidine; Hot Temperature; Hydrogen-Ion Concentration; Papain; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Urea | 2002 |
The unfolding mechanism and the disulfide structures of denatured lysozyme.
Topics: Animals; Chickens; Chromatography, High Pressure Liquid; Disulfides; Enzyme Stability; Guanidine; Guanidines; Isomerism; Mercaptoethanol; Muramidase; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Tertiary; Temperature; Thiocyanates; Urea | 2002 |
The effect of high protein diet on urea and guanidino compound levels in renal insufficient mice.
Topics: Animals; Body Weight; Chromatography, Gas; Dietary Proteins; Guanidine; Male; Mice; Mice, Inbred Strains; Nephrectomy; Renal Insufficiency; Urea | 2001 |
Guanidinium chloride- and urea-induced unfolding of the dimeric enzyme glucose oxidase.
Topics: Calorimetry, Differential Scanning; Chromatography, Gel; Circular Dichroism; Dimerization; Glucose Oxidase; Guanidine; Protein Denaturation; Sodium Chloride; Spectrometry, Fluorescence; Urea | 2002 |
The unfolding pathway of leech carboxypeptidase inhibitor.
Topics: Amino Acid Sequence; Animals; Disulfides; Guanidine; Guanidines; Isothiocyanates; Leeches; Molecular Sequence Data; Protein Conformation; Protein Denaturation; Protein Folding; Proteins; Spectrometry, Fluorescence; Urea | 2002 |
Activation of polyphenol oxidase in extracts of bran from several wheat (Triticum aestivum) cultivars using organic solvents, detergents, and chaotropes.
Topics: 1-Butanol; 2-Propanol; Catechol Oxidase; Detergents; Enzyme Activation; Ethanol; Guanidine; Plant Extracts; Sodium Dodecyl Sulfate; Solvents; Triticum; Urea | 2002 |
Cracking the folding code. Why do some proteins adopt partially folded conformations, whereas other don't?
Topics: Amino Acid Sequence; Computational Biology; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Proteins; Temperature; Urea | 2002 |
Partially folded conformations in the folding pathway of bovine carbonic anhydrase II: a fluorescence spectroscopic analysis.
Topics: Animals; Carbonic Anhydrase II; Cattle; Fluorescence Polarization; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Spectrometry, Fluorescence; Time Factors; Urea | 2001 |
Exploring the role of alanine in the structure of the Lac repressor tetramerization domain, a ferritin-like Alacoil.
Topics: Alanine; Amino Acid Sequence; Bacterial Proteins; Circular Dichroism; Escherichia coli Proteins; Ferritins; Guanidine; Lac Repressors; Models, Molecular; Molecular Sequence Data; Mutation; Protein Denaturation; Protein Structure, Quaternary; Protein Structure, Tertiary; Repressor Proteins; Sequence Alignment; Sodium Chloride; Temperature; Thermodynamics; Ultracentrifugation; Urea | 2002 |
Isolation and characterization of a polymerized prion protein.
Topics: Animals; Chromatography, High Pressure Liquid; Circular Dichroism; Disulfides; Endopeptidase K; Guanidine; Hydrogen-Ion Concentration; Light; Mice; Peptide Fragments; Prions; Protein Conformation; Protein Isoforms; Protein Structure, Secondary; Scattering, Radiation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors; Ultraviolet Rays; Urea | 2002 |
Activity and conformational changes of horseradish peroxidase in trifluoroethanol.
Topics: Binding Sites; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Guanidine; Horseradish Peroxidase; Hydrogen Bonding; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteins; Spectrometry, Fluorescence; Trifluoroethanol; Trypsinogen; Urea | 2002 |
pH corrections in chemical denaturant solutions.
Topics: Electrochemistry; Guanidine; Hydrogen-Ion Concentration; Osmolar Concentration; Protein Denaturation; Solutions; Thermodynamics; Urea; Water | 2002 |
Conformational changes at the active site of adenylate kinase detected using a fluorescent probe and monoclonal antibody binding.
Topics: Adenylate Kinase; Animals; Antibodies, Monoclonal; Binding Sites; Cysteine; Fluorescence; Fluorescent Dyes; Guanidine; Protein Binding; Protein Conformation; Protein Denaturation; Rabbits; Time Factors; Urea | 2002 |
Two beta-rich structural domains in GABA(A) receptor alpha(1) subunit with different physical properties: Evidence for multidomain nature of the receptor.
Topics: Animals; Cattle; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Guanidine; Hydrogen-Ion Concentration; Lasers; Molecular Weight; Mutagenesis, Site-Directed; Peptide Fragments; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Subunits; Receptors, GABA-A; Scattering, Radiation; Spectroscopy, Fourier Transform Infrared; Urea | 2002 |
The denaturation of circular enterocin AS-48 by urea and guanidinium hydrochloride.
Topics: Bacteriocins; Bridged-Ring Compounds; Circular Dichroism; Enterococcus faecalis; Guanidine; Hot Temperature; Kinetics; Mathematics; Models, Molecular; Protein Conformation; Protein Denaturation; Thermodynamics; Urea | 2002 |
Denaturing action of urea and guanidine hydrochloride towards two thermophilic esterases.
Topics: Archaeoglobus; Bacillus; Circular Dichroism; Esterases; Guanidine; Protein Denaturation; Spectrometry, Fluorescence; Urea | 2002 |
Subunit interface mutation disrupting an aromatic cluster in Plasmodium falciparum triosephosphate isomerase: effect on dimer stability.
Topics: Animals; Chromatography; Circular Dichroism; Dimerization; Energy Transfer; Enzyme Stability; Glyceraldehyde 3-Phosphate; Guanidine; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Plasmodium falciparum; Point Mutation; Protein Folding; Protein Subunits; Spectrometry, Fluorescence; Spectrometry, Mass, Electrospray Ionization; Triose-Phosphate Isomerase; Urea | 2002 |
Effect of chaotropic agents on the structure-function of recombinant acylpeptide hydrolase.
Topics: Animals; Chromatography, Gel; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Guanidine; Kinetics; Molecular Weight; Peptide Hydrolases; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Structure-Activity Relationship; Swine; Urea | 2002 |
Acid and chemical induced conformational changes of ervatamin B. Presence of partially structured multiple intermediates.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Cysteine Endopeptidases; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectrophotometry; Urea | 2002 |
Different unfolding pathways for mesophilic and thermophilic homologues of serine hydroxymethyltransferase.
Topics: Amino Acid Sequence; Aspartate Aminotransferases; Bacillus subtilis; Chromatography; Circular Dichroism; Enzyme Stability; Fluorescent Dyes; Glutaral; Glycine Hydroxymethyltransferase; Guanidine; Hot Temperature; Molecular Sequence Data; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Tertiary; Pyridoxal Phosphate; Sequence Alignment; Sequence Homology; Urea | 2002 |
Rapid liquid-phase combinatorial synthesis of heterocyclic libraries.
Topics: Benzimidazoles; Combinatorial Chemistry Techniques; Guanidine; Guanidines; Heterocyclic Compounds; Magnetic Resonance Spectroscopy; Piperidines; Urea | 2002 |
Protein stabilization by urea and guanidine hydrochloride.
Topics: Animals; Carbon Monoxide; Cytochrome c Group; Drug Synergism; Entropy; Enzyme Activation; Guanidine; Horses; Kinetics; Models, Chemical; Protein Conformation; Protein Denaturation; Protein Folding; Sodium Chloride; Solvents; Spectrometry, Fluorescence; Spectrophotometry; Sulfates; Temperature; Urea | 2002 |
Equilibrium unfolding and conformational plasticity of troponin I and T.
Topics: Anilino Naphthalenesulfonates; Animals; Chickens; Circular Dichroism; Escherichia coli; Fluorescent Dyes; Guanidine; Muscles; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Troponin I; Troponin T; Urea | 2002 |
Renaturation of human proinsulin--a study on refolding and conversion to insulin.
Topics: Carboxypeptidase B; Carboxypeptidases; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Cystine; Enzyme-Linked Immunosorbent Assay; Guanidine; Humans; Hydrogen-Ion Concentration; Insulin; Oxidation-Reduction; Proinsulin; Protein Denaturation; Protein Renaturation; Protein Structure, Secondary; Recombinant Proteins; Temperature; Trypsin; Urea | 2002 |
Characterization of the unfolding process of lipocalin-type prostaglandin D synthase.
Topics: Animals; Circular Dichroism; Guanidine; Intramolecular Oxidoreductases; Kinetics; Lipocalins; Magnetic Resonance Spectroscopy; Mice; Protein Conformation; Protein Denaturation; Protein Folding; Urea | 2003 |
Structural and thermodynamic studies on a salt-bridge triad in the NADP-binding domain of glutamate dehydrogenase from Thermotoga maritima: cooperativity and electrostatic contribution to stability.
Topics: Alanine; Arginine; Binding Sites; Crystallography, X-Ray; Cysteine; Enzyme Stability; Glutamate Dehydrogenase; Glutamic Acid; Guanidine; Lysine; Mutagenesis, Site-Directed; NADP; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Recombinant Proteins; Salts; Serine; Static Electricity; Structure-Activity Relationship; Thermodynamics; Thermotoga maritima; Urea | 2002 |
Structural changes enhance the activity of Chainia xylanase in low urea concentrations.
Topics: Acrylamide; Actinomyces; Circular Dichroism; Enzyme Activation; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Spectrometry, Fluorescence; Thermodynamics; Urea; Xylan Endo-1,3-beta-Xylosidase; Xylosidases | 2003 |
Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants.
Topics: Animals; Circular Dichroism; Fluorescence; Glycolates; Guanidine; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Plasmodium falciparum; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Triose-Phosphate Isomerase; Tryptophan; Urea | 2003 |
Spectroscopic analysis of the stability of bothrops myotoxic phospholipases A2 to guanidine and urea denaturation.
Topics: Animals; Bothrops; Cattle; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Guanidine; Phospholipases A; Protein Denaturation; Ribonuclease, Pancreatic; Spectrophotometry, Ultraviolet; Thermodynamics; Trypsin; Urea | 2003 |
Observed hysteresis of virus capsid disassembly is implicit in kinetic models of assembly.
Topics: Capsid; Dimerization; Dose-Response Relationship, Drug; Escherichia coli; Guanidine; Hepatitis B Core Antigens; Hepatitis B virus; Kinetics; Light; Scattering, Radiation; Thermodynamics; Time Factors; Tryptophan; Urea; Viral Core Proteins; Virion; Virus Assembly | 2003 |
Cytochrome c(551) as a model system for protein folding.
Topics: Bacterial Proteins; Cytochrome c Group; Guanidine; Kinetics; Models, Chemical; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Urea | 2003 |
Detection of an intermediate during unfolding of bacterial cell division protein FtsZ: loss of functional properties precedes the global unfolding of FtsZ.
Topics: Anilino Naphthalenesulfonates; Bacterial Proteins; Circular Dichroism; Cytoskeletal Proteins; Dose-Response Relationship, Drug; Escherichia coli; Fluorescent Dyes; GTP Phosphohydrolases; Guanidine; Hydrolysis; Light; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Scattering, Radiation; Sodium Dodecyl Sulfate; Spectrophotometry; Surface-Active Agents; Temperature; Time Factors; Ultraviolet Rays; Urea | 2003 |
Equilibrium unfolding of RNase Rs from Rhizopus stolonifer: pH dependence of chemical and thermal denaturation.
Topics: Circular Dichroism; Enzyme Stability; Guanidine; Hot Temperature; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Rhizopus; Ribonucleases; Spectrometry, Fluorescence; Urea | 2003 |
Folding and stability of the extracellular domain of the human amyloid precursor protein.
Topics: Amyloid; Anilino Naphthalenesulfonates; Circular Dichroism; Dose-Response Relationship, Drug; Guanidine; Humans; Kinetics; Ligands; Microscopy, Fluorescence; Protein Conformation; Protein Folding; Protein Isoforms; Protein Structure, Tertiary; Scattering, Radiation; Ultraviolet Rays; Urea; X-Rays | 2003 |
Protein stability in mixed solvents: a balance of contact interaction and excluded volume.
Topics: Algorithms; Binding Sites; Complex Mixtures; Computer Simulation; Databases, Protein; Guanidine; Methylamines; Models, Chemical; Models, Molecular; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Proteins; Solvents; Sucrose; Surface Properties; Urea; Water | 2003 |
Unfolding of Vibrio cholerae hemolysin induces oligomerization of the toxin monomer.
Topics: Cell Membrane; Cholesterol; Diffusion; Electrophoresis, Polyacrylamide Gel; Guanidine; Hemolysin Proteins; Lipid Metabolism; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Sphingolipids; Thermodynamics; Toxins, Biological; Tryptophan; Urea; Vibrio cholerae | 2003 |
[Modifications of the agglutinating power of a human O serum towards A and B erythrocytes under the influence of sodium salicylate, urea and guanidine hydrochloride].
Topics: Agglutination; Amidines; Erythrocytes; Guanidine; Hemagglutination; Hemagglutination Tests; Humans; Salicylates; Sodium Salicylate; Urea | 1954 |
[Edestin modification by urea and guanidinium ion].
Topics: Amidines; Cannabis; Globulins; Guanidine; Plant Proteins; Urea | 1954 |
Further studies on the denaturation of arachin with urea and guanidinium chloride.
Topics: Globulins; Guanidine; Humans; Plant Proteins; Urea | 1958 |
The effect of urea and guanidine hydrochloride on activity and optical rotation of crystalline papain.
Topics: Amidines; Endopeptidases; Guanidine; Hydrolases; Optical Rotation; Papain; Peptide Hydrolases; Urea | 1959 |
[Carbamoyl and guanidine compounds in plants].
Topics: Amidines; Guanidine; Guanidines; Urea | 1959 |
The effect of urea and guanidine hydrochloride on activity and optical rotation of penicillinase.
Topics: Amidines; Guanidine; Optical Rotation; Penicillinase; Urea | 1960 |
Effect of denaturing agents of the urea-guanidinium class on the solubility of acetyltetraglycine ethyl ester and related compounds.
Topics: Esters; Guanidine; Guanidines; Peptides; Solubility; Urea | 1963 |
Characteristics of kappa-casein in the presence of various dissociating agents.
Topics: Caseins; Guanidine; Guanidines; Humans; Urea | 1963 |
The effect of urea and guanidine on the helix content of poly-N5-(3-hydroxypropyl)-L-glutamine in aqueous-solvent systems.
Topics: Glutamine; Guanidine; Guanidines; Protein Structure, Secondary; Solvents; Urea; Water | 1963 |
The role of solvent-induced conformational changes on the enzymatic adenosine triphosphatase activity of cardiac myosin A.
Topics: Adenosine Triphosphatases; Alcohols; Cardiac Myosins; Dioxanes; Glycols; Guanidine; Guanidines; Heart; Myocardium; Nonmuscle Myosin Type IIA; Solvents; Urea | 1962 |
Inhibition of kidney mutarotase activity by glucose, urea and guanidine hydrochloride.
Topics: Carbohydrate Epimerases; Glucose; Guanidine; Isomerases; Kidney; Urea | 1963 |
[Action of various aromatic substances and nitrogen metabolites on heme synthesis in vitro].
Topics: Creatine; Creatinine; Guanidine; Guanidines; Heme; In Vitro Techniques; Indoles; Nitrogen; Phenols; Urea; Uric Acid | 1963 |
ISOCITRIC-DEHYDROGENASE OF RABBIT RETICULOCYTES AND ERYTHROCYTES. II. THE INFLUENCE OF PHYSICAL AND CHEMICAL AGENTS ON THE LIBERATION OF THE ENZYME; THE MAXIMAL ACTIVITY OF THE ENZYME IN THE CELL.
Topics: Animals; Cold Temperature; Cysteine; Erythrocytes; Glutathione; Guanidine; Guanidines; Isocitrate Dehydrogenase; Lagomorpha; Oxidoreductases; Physical Examination; Rabbits; Research; Reticulocytes; Sucrose; Urea | 1963 |
STRUCTURE OF MURAMIDASE (LYSOZYME). I. THE EFFECT OF GUANIDINE HYDROCHLORIDE ON MURAMIDASE.
Topics: Chemical Phenomena; Chemistry; Guanidine; Guanidines; Muramidase; Research; Spectrophotometry; Urea | 1963 |
THE EFFECT OF UREA AND GUANIDINE HYDROCHLORIDE ON THE PAPAIN-CATALYZED SYNTHESIS OF HIPPURYL-M-TOLUIDIDE.
Topics: Chemical Phenomena; Chemistry; Chlorides; Guanidine; Guanidines; Hippurates; Papain; Research; Toluene; Urea | 1963 |
[THE PROBLEM OF THE SPECIFICITY OF GUANIDINE FOR THE DEVELOPMENT OF A GUANIDINE-REQUIRING MUTANT OF POLIOVIRUS].
Topics: Adenine; Amino Acids; Arginine; Creatine; Creatinine; Enterovirus C, Human; Glycine; Guanidine; Guanidines; Hydrazines; Metabolism; Mutation; Ornithine; Poliovirus; Research; Uracil; Urea | 1963 |
[EFFECTS OF GUANIDINE AND UREA ON VARIOUS SENSITIVE, RESISTANT AND REQUIRING POLIOVIRUS STRAINS].
Topics: Drug Resistance; Drug Resistance, Microbial; Enterovirus C, Human; Guanidine; Guanidines; Metabolism; Mutation; Poliovirus; Research; Urea | 1963 |
STRUCTURAL TRANSITIONS OF LYSOZYME.
Topics: Chemistry Techniques, Analytical; Fluorescence; Guanidine; Guanidines; Hydrogen-Ion Concentration; Muramidase; Research; Spectrum Analysis; Temperature; Tryptophan; Urea | 1964 |
[REMARKS ON ISATIN-BETA-THIOSEMICARBAZONE AND ON SEVERAL INHIBITORS OF VIRAL DEVELOPMENT].
Topics: Antiviral Agents; Arginine; Citrulline; Creatine; Creatinine; Enterovirus C, Human; Glutamine; Glycine; Guanidine; Guanidines; Indoles; Isatin; Mutation; Ornithine; Pharmacology; Poliovirus; Thiosemicarbazones; Thiourea; Uracil; Urea | 1964 |
ISOZYME HISTOCHEMISTRY: A NEW METHOD FOR THE DISPLAY OF SELECTIVE LACTATE DEHYDROGENASE ISOZYMES ON AN ELECTROPHORETIC PATTERN.
Topics: Animals; Electrophoresis; Enzyme Inhibitors; Guanidine; Guanidines; Guinea Pigs; Histocytochemistry; Isoenzymes; L-Lactate Dehydrogenase; Liver; Muscles; Pharmacology; Physiology, Comparative; Research; Species Specificity; Urea | 1964 |
[STUDIES ON HABU-SNAKE VENOM].
Topics: Animals; Crotalid Venoms; Cysteine; Ecchymosis; Formaldehyde; Glucuronates; Guanidine; Guanidines; Hemolysis; Injections, Intraperitoneal; Injections, Subcutaneous; Lagomorpha; Mice; Pharmacology; Rabbits; Research; Salicylates; Snake Venoms; Snakes; Sulfates; Thioglycolates; Toxicology; Trimeresurus; Urea; Venoms | 1963 |
OXYGEN-SENSITIVE REACTIONS OF PROTEINS AND PEPTIDES. II. THE REACTIVE BASE.
Topics: Amines; Amino Alcohols; Ammonia; Animals; Colorimetry; Cystine; Ethylamines; Ethylenediamines; Feathers; Guanidine; Guanidines; Hydrazines; Keratins; Oxygen; Peptides; Proteins; Research; Urea; Wool | 1964 |
STRUCTURE OF MURAMIDASE (LYSOZYME). VI. EFFECT OF AMIDE DERIVATIVES AND THE STRUCTURE OF INTERNAL FOLD OF THE MURAMIDASE MOLECULE.
Topics: Amides; Chemical Phenomena; Chemistry; Guanidine; Guanidines; Muramidase; Research; Solvents; Urea; Viscosity | 1964 |
EFFECT OF GUANIDINIUM SALTS ON THE TOXICITY OF BOTULINUM TOXIN.
Topics: Acetates; Animals; Antitoxins; Benzoates; Botulinum Toxins; Chemical Phenomena; Chemistry; Chlorides; Citrates; Clostridium botulinum; Fluorides; Fumarates; Glutarates; Guanidine; Guanidines; Malates; Malonates; Mice; Phosphates; Phthalic Acids; Research; Salts; Succinates; Sulfates; Tartrates; Toxicology; Toxins, Biological; Urea | 1964 |
THE EFFECT OF PYRIDINE DERIVATIVES ON THE ACTIVITY OF CRYSTALLINE RIBONUCLEASE.
Topics: Guanidine; Guanidines; Isoniazid; Isonicotinic Acids; Niacin; Niacinamide; Nicotinic Acids; Phosphates; Pyridines; Research; Ribonucleases; RNA; Solubility; Urea | 1964 |
ALTERATIONS IN THE PHYSICAL AND ENZYMIC PROPERTIES OF ALPHA-CHYMOTRYPSIN IN UREA AND GUANIDINIUM CHLORIDE.
Topics: Calcium Chloride; Chemical Phenomena; Chemistry; Chymotrypsin; Guanidine; Guanidines; Hydrogen-Ion Concentration; Research; Spectrophotometry; Urea | 1964 |
COMPETITIVE INHIBITION OF XANTHINE OXIDASE BY UREA AND GUANIDINIUM ION.
Topics: Enzyme Inhibitors; Guanidine; Guanidines; Kinetics; Oxygen; Research; Thiocyanates; Urea; Xanthine Oxidase | 1964 |
COHESIVE FORCES AND DISRUPTIVE REAGENTS.
Topics: Alcohols; Chemical Phenomena; Chemistry; Chemistry, Physical; Ethanol; Glycols; Guanidine; Guanidines; Hemoglobins; Indicators and Reagents; Proteins; Sodium Chloride; Solvents; Terminology as Topic; Thermodynamics; Urea | 1964 |
A PILOT TRIAL OF CI-515 IN SCHIZOPHRENIC PATIENTS.
Topics: Blood Cell Count; Blood Urea Nitrogen; Drug Therapy; Electrocardiography; Guanidine; Guanidines; Liver Function Tests; Photosensitivity Disorders; Schizophrenia; Toxicology; Urea; Urine | 1965 |
THE DEAGGREGATION OF BOVINE LENS ALPHA-CRYSTALLIN.
Topics: alpha-Crystallins; Animals; Biochemical Phenomena; Biochemistry; Cattle; Crystallins; Eye Proteins; Guanidine; Guanidines; Hydrogen-Ion Concentration; Lens, Crystalline; Molecular Weight; Research; Succinates; Ultracentrifugation; Urea | 1965 |
SYNERGISTIC INHIBITION OF XANTHINE OXIDASE BY GUANIDINIUM PLUS THIOCYANATE.
Topics: Chemical Phenomena; Chemistry; Enzyme Inhibitors; Guanidine; Guanidines; Hydrogen-Ion Concentration; Research; Thiocyanates; Urea; Xanthine Oxidase | 1965 |
ISOZYME HISTOCHEMISTRY, ESPECIALLY IN RELATION TO MALIGNANT TRANSFORMATION AND DIFFERENTIATION. I. HISTOCHEMICAL DEMONSTRATION OF LACTIC DEHYDROGENASE ISOZYMES.
Topics: Animals; Electrophoresis; Guanidine; Guanidines; Guinea Pigs; Histocytochemistry; Hot Temperature; Isoenzymes; L-Lactate Dehydrogenase; Poultry; Rats; Urea | 1965 |
ACTIVITY OF CONGENERS OF HYDROXYUREA AGAINST ADVANCED LEUKEMIA L1210.
Topics: Animals; Guanidine; Guanidines; Hydrazines; Hydroxylamines; Hydroxyurea; Leukemia L1210; Mice; Pharmacology; Research; Selenium; Selenium Compounds; Thiourea; Urea; Urethane | 1965 |
THE EFFECT OF COMPOUNDS OF THE UREA-GUANIDINIUM CLASS ON THE ACTIVITY COEFFICIENT OF ACETYLTETRAGLYCINE ETHYL ESTER AND RELATED COMPOUNDS.
Topics: Chemical Phenomena; Chemistry; Esters; Guanidine; Guanidines; Peptides; Protein Denaturation; Research; Urea | 1965 |
STUDIES OF SOME SUBSTANCES WITH ANTIVIRAL ACTIVITY. I. THE INHIBITORY EFFECT OF GUANIDINE ON THE GROWTH OF HERPES SIMPLEX VIRUS IN HUMAN EMBRYONIC KIDNEY CELL CULTURES.
Topics: Antiviral Agents; Arginine; Creatine; Creatinine; Cytopathogenic Effect, Viral; DNA; DNA, Viral; Guanidine; Guanidines; Pharmacology; Research; Simplexvirus; Thiourea; Tissue Culture Techniques; Urea; Virus Cultivation | 1965 |
LOCATION OF ABNORMAL TYROSINES IN ACTIN.
Topics: Actins; Guanidine; Guanidines; Hydrogen-Ion Concentration; Muscle Proteins; Research; Spectrophotometry; Tyrosine; Ultracentrifugation; Urea | 1965 |
[Study of anaphylactic shock in the guinea pig; modifications of the releasing power of horse serum by chemical treatment; action of sodium salicylate, various substances related to salicylate, urea, guanidine, formol, etc..].
Topics: Anaphylaxis; Formaldehyde; Guanidine; Guanidines; Guanine; Guinea Pigs; Hypersensitivity; Salicylates; Serum; Sodium Salicylate; Urea | 1955 |
[Studies on several reactions with alkyl vinyl ethers and their derivatives. II. Reactions of 1,1,3,3-tetraalkoxypropane with guanidine, guanidine derivatives, and urea].
Topics: Amidines; Antihypertensive Agents; Ethers; Guanidine; Guanidines; Urea; Vinyl Compounds | 1962 |
Probing the interface in a human co-chaperonin heptamer: residues disrupting oligomeric unfolded state identified.
Topics: Chaperonin 10; Guanidine; Humans; Macromolecular Substances; Phenylalanine; Point Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Subunits; Temperature; Urea; Valine | 2003 |
Differences in the unfolding of procerain induced by pH, guanidine hydrochloride, urea, and temperature.
Topics: Cysteine Endopeptidases; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Temperature; Thermodynamics; Urea | 2003 |
Kinetic inactivation study of mushroom tyrosinase: intermediate detection by denaturants.
Topics: Agaricus; Catalysis; Guanidine; Kinetics; Monophenol Monooxygenase; Protein Denaturation; Protein Folding; Sodium Dodecyl Sulfate; Thermodynamics; Urea | 2003 |
Folding mechanism of FIS, the intertwined, dimeric factor for inversion stimulation.
Topics: Circular Dichroism; Dimerization; Factor For Inversion Stimulation Protein; Guanidine; Kinetics; Models, Molecular; Molecular Weight; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Thermodynamics; Urea | 2004 |
The side chain of aspartic acid 69 dictates the folding mechanism of Bacillus subtilis HPr.
Topics: Alanine; Amino Acid Substitution; Aspartic Acid; Bacillus subtilis; Bacterial Proteins; Calorimetry, Differential Scanning; Circular Dichroism; Deuterium Exchange Measurement; Guanidine; Kinetics; Protein Denaturation; Protein Folding; Salts; Sodium Chloride; Solvents; Thermodynamics; Transcription Factors; Urea | 2004 |
Low molecular mass pectate lyase from Fusarium moniliforme: similar modes of chemical and thermal denaturation.
Topics: Binding, Competitive; Dose-Response Relationship, Drug; Fusarium; Guanidine; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Polysaccharide-Lyases; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Thermodynamics; Time Factors; Urea; Water | 2004 |
Blood levels of urea nitrogen, phenol, guanidine and creatinine in uremia.
Topics: Blood; Creatinine; Guanidine; Guanidines; Humans; Nitrogen; Phenol; Phenols; Urea; Uremia | 1951 |
Effect of signal peptide on the stability and folding kinetics of maltose binding protein.
Topics: Bacterial Proteins; Carrier Proteins; Circular Dichroism; Escherichia coli Proteins; Guanidine; Hydrolysis; Kinetics; Maltose-Binding Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Protein Precursors; Protein Renaturation; Protein Sorting Signals; Spectrometry, Fluorescence; Substrate Specificity; Temperature; Thermodynamics; Urea | 2004 |
Monomer topology defines folding speed of heptamer.
Topics: Chaperonin 10; Fluorescence; Guanidine; Humans; Kinetics; Protein Folding; Urea | 2004 |
Effect of chaotropic agents on reversible unfolding of a soybean (Glycine max) seed acid phosphatase.
Topics: Acid Phosphatase; Glycine max; Guanidine; Protein Denaturation; Protein Folding; Seeds; Spectrometry, Fluorescence; Thermodynamics; Urea | 2004 |
The NMR structure of a stable and compact all-beta-sheet variant of intestinal fatty acid-binding protein.
Topics: Carrier Proteins; Fatty Acid-Binding Proteins; Guanidine; Nuclear Magnetic Resonance, Biomolecular; Oleic Acid; Protein Binding; Protein Denaturation; Protein Structure, Secondary; Spectrometry, Fluorescence; Urea | 2004 |
Characterization of bis(levuglandinyl) urea derivatives as products of the reaction between prostaglandin H2 and arginine.
Topics: Aldehydes; Amyloid beta-Peptides; Arginine; Guanidine; Histones; Humans; Lysine; Ornithine; Peptide Fragments; Prostaglandin H2; Prostaglandins E; Schiff Bases; Serum Albumin; Spectrometry, Mass, Electrospray Ionization; Urea | 2004 |
Small amounts of urea and guanidine hydrochloride can be detected by a far-UV spectrophotometric method in dialysed protein solutions.
Topics: Complex Mixtures; Dialysis Solutions; Guanidine; Microchemistry; Proteins; Reference Values; Refractometry; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Urea | 2004 |
Is an intermediate state populated on the folding pathway of ubiquitin?
Topics: Amino Acid Sequence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Potassium Chloride; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Sulfates; Ubiquitin; Urea | 2004 |
Global analysis of three-state protein unfolding data.
Topics: Animals; Circular Dichroism; Guanidine; Humans; Macrophage Colony-Stimulating Factor; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Retinoid X Receptor alpha; Spectrometry, Fluorescence; Urea | 2004 |
Random-coil behavior and the dimensions of chemically unfolded proteins.
Topics: Guanidine; Humans; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteins; Thermodynamics; Urea | 2004 |
Low stability of nucleocapsid protein in SARS virus.
Topics: Cations, Monovalent; Guanidine; Hot Temperature; Humans; Hydrogen-Ion Concentration; Nucleocapsid Proteins; Protein Denaturation; Protein Folding; Severe Acute Respiratory Syndrome; Severe acute respiratory syndrome-related coronavirus; Sodium Chloride; Thermodynamics; Urea; Virus Inactivation | 2004 |
Effect of metal ion binding on the structural stability of the hepatitis C virus RNA polymerase.
Topics: Alanine; DNA-Directed RNA Polymerases; Enzyme Stability; Guanidine; Hepacivirus; Metals; Mutation; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Thermodynamics; Urea; Viral Proteins | 2004 |
[Guanidine and azotemic intoxication: clinical value of the correlation between guanidine and urea in uremic and eclamptic syndromes].
Topics: Blood; Guanidine; Guanidines; Syndrome; Urea | 1949 |
Thermodynamics of denaturant-induced unfolding of a protein that exhibits variable two-state denaturation.
Topics: Bacterial Proteins; Circular Dichroism; Guanidine; Hydrochloric Acid; Light; Linear Energy Transfer; Models, Chemical; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Protons; Recombinant Proteins; Scattering, Radiation; Spectrometry, Fluorescence; Streptococcus; Thermodynamics; Titrimetry; Urea | 2004 |
The Kirkwood-Buff theory and the effect of cosolvents on biochemical reactions.
Topics: Animals; Chemistry, Physical; Chickens; Guanidine; Ligands; Models, Statistical; Muramidase; Protein Binding; Protein Conformation; Protein Denaturation; Proteins; Solvents; Thermodynamics; Urea; Water | 2004 |
Denaturant-induced unfolding of the acetyl-esterase from Escherichia coli.
Topics: Acetylesterase; Aspartic Acid; Carboxylic Ester Hydrolases; Circular Dichroism; Enzyme Stability; Escherichia coli Proteins; Guanidine; Models, Molecular; Point Mutation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Sodium Chloride; Spectrometry, Fluorescence; Structural Homology, Protein; Thermodynamics; Urea; Valine | 2004 |
Molecular basis for the effect of urea and guanidinium chloride on the dynamics of unfolded polypeptide chains.
Topics: Guanidine; Molecular Structure; Peptides; Protein Conformation; Protein Denaturation; Protein Folding; Urea; Viscosity; Xanthones | 2005 |
Equilibrium unfolding of dimeric and engineered monomeric forms of lambda Cro (F58W) repressor and the effect of added salts: evidence for the formation of folded monomer induced by sodium perchlorate.
Topics: Bacteriophage lambda; Circular Dichroism; Dimerization; DNA-Binding Proteins; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Perchlorates; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Protein Structure, Quaternary; Recombinant Proteins; Repressor Proteins; Sodium Chloride; Sodium Compounds; Static Electricity; Thermodynamics; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 2005 |
Unfolding and refolding pathways of a major kinetic trap in the oxidative folding of alpha-lactalbumin.
Topics: Animals; Cattle; Chromatography, High Pressure Liquid; Circular Dichroism; Disulfides; Guanidine; Hot Temperature; Isomerism; Kinetics; Lactalbumin; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Folding; Protein Isoforms; Signal Transduction; Urea | 2005 |
Dissecting contributions to the denaturant sensitivities of proteins.
Topics: Amino Acid Sequence; Circular Dichroism; Guanidine; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Solutions; Thermodynamics; Tryptophan; Urea | 2005 |
Cloning, expression, and refolding of a secretory protein ESAT-6 of Mycobacterium tuberculosis.
Topics: Antigens, Bacterial; Bacterial Proteins; Blotting, Western; Centrifugation; Chromatography, Gel; Cloning, Molecular; Dialysis; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Expression; Genetic Vectors; Guanidine; Histidine; Hydrogen-Ion Concentration; Inclusion Bodies; Mycobacterium tuberculosis; Plasmids; Protein Renaturation; Recombinant Fusion Proteins; Reference Standards; Solubility; Sonication; Urea | 2005 |
The fusion-controlling disulfide bond isomerase in retrovirus Env is triggered by protein destabilization.
Topics: Animals; Cell Line; Cricetinae; Disulfides; Gene Expression Regulation, Viral; Gene Products, env; Guanidine; Hot Temperature; Membrane Fusion; Mice; Moloney murine leukemia virus; Protein Disulfide-Isomerases; Stereoisomerism; Urea | 2005 |
Effects of heme on the structure of the denatured state and folding kinetics of cytochrome b562.
Topics: Amino Acid Sequence; Apoproteins; Circular Dichroism; Cytochrome b Group; Escherichia coli; Escherichia coli Proteins; Guanidine; Heme; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Molecular Sequence Data; Mutation; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Thermodynamics; Tryptophan; Urea | 2005 |
Apparent Debye-Huckel electrostatic effects in the folding of a simple, single domain protein.
Topics: Bromides; Guanidine; Kinetics; Osmolar Concentration; Potassium Chloride; Potassium Compounds; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fyn; Sodium Chloride; Sodium Compounds; Solvents; src Homology Domains; src-Family Kinases; Static Electricity; Thermodynamics; Urea | 2005 |
Preparation and characterization of geodin. A betagamma-crystallin-type protein from a sponge.
Topics: Amino Acid Sequence; Animals; beta-Crystallins; Calcium; Cloning, Molecular; Escherichia coli Proteins; gamma-Crystallins; Guanidine; Models, Molecular; Molecular Sequence Data; Porifera; Protein Structure, Tertiary; Recombinant Proteins; Sequence Alignment; Temperature; Urea | 2005 |
FMN binding and unfolding of Desulfovibrio desulfuricans flavodoxin: "hidden" intermediates at low denaturant concentrations.
Topics: Circular Dichroism; Desulfovibrio desulfuricans; Flavin Mononucleotide; Flavodoxin; Guanidine; Protein Conformation; Protein Denaturation; Protein Folding; Thermodynamics; Urea | 2005 |
Conformational study of spectrin in presence of submolar concentrations of denaturants.
Topics: 2-Naphthylamine; Animals; Binding Sites; Dimerization; Fluorescence Polarization; Fluorescent Dyes; Guanidine; In Vitro Techniques; Protein Conformation; Protein Denaturation; Protein Structure, Quaternary; Sheep; Spectrin; Tryptophan; Urea | 2005 |
Two-state folding of horse ferrocytochrome c: analyses of linear free energy relationship, chevron curvature, and stopped-flow burst relaxation kinetics.
Topics: Animals; Cytochromes c; Guanidine; Horses; Indicators and Reagents; Kinetics; Protein Denaturation; Protein Folding; Thermodynamics; Urea | 2005 |
[Investigation of alpha-amylase denatured by urea and guanidine hydrochloride. I. Studies on denaturation mechanism and renaturation efficiency by high-performance hydrophobic interaction chromatography].
Topics: alpha-Amylases; Animals; Cattle; Chromatography, Liquid; Guanidine; Hydrophobic and Hydrophilic Interactions; Protein Denaturation; Urea | 1997 |
Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability.
Topics: Disulfides; Guanidine; Immunoglobulin Fab Fragments; Immunoglobulin Heavy Chains; Kinetics; Models, Molecular; Protein Binding; Protein Denaturation; Protein Engineering; Protein Folding; Protein Structure, Quaternary; Protein Structure, Tertiary; Thermodynamics; Urea | 2005 |
Kinetic stabilization of the native state by protein engineering: implications for inhibition of transthyretin amyloidogenesis.
Topics: Amyloid; Crystallography, X-Ray; Guanidine; Kinetics; Ligands; Models, Molecular; Prealbumin; Protein Binding; Protein Denaturation; Protein Engineering; Protein Structure, Quaternary; Protein Structure, Tertiary; Protein Subunits; Spectrometry, Fluorescence; Urea | 2005 |
Folding and stability of a coiled-coil investigated using chemical and physical denaturing agents: comparative analysis of polymerized and non-polymerized forms of alpha-tropomyosin.
Topics: Anilino Naphthalenesulfonates; Animals; Chickens; Dimerization; Dipeptides; Guanidine; Hydrophobic and Hydrophilic Interactions; Hydrostatic Pressure; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Static Electricity; Temperature; Tropomyosin; Urea | 2005 |
Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase: stabilization of an apo-protein by GdmCl.
Topics: Circular Dichroism; Enzyme Stability; Guanidine; NADH, NADPH Oxidoreductases; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Static Electricity; Urea | 2005 |
A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea.
Topics: Anilino Naphthalenesulfonates; Bacterial Proteins; Cell Division; Cytoskeletal Proteins; Fluorescent Dyes; Glutamic Acid; GTP Phosphohydrolases; Guanidine; Methylamines; Parasympathomimetics; Protein Conformation; Protein Denaturation; Protein Folding; Urea | 2005 |
Interface mutation in heptameric co-chaperonin protein 10 destabilizes subunits but not interfaces.
Topics: Amino Acid Sequence; Amino Acid Substitution; Chaperonin 10; Circular Dichroism; Conserved Sequence; Cross-Linking Reagents; Dimerization; Fluorescence; Glycine; Guanidine; Humans; Leucine; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Subunits; Ultracentrifugation; Urea | 2005 |
[Studies on formation of aggregates from denatured lysozymes upon renaturing with size exclusion chromatography].
Topics: Animals; Chickens; Chromatography, Gel; Chromatography, High Pressure Liquid; Egg Proteins; Egg White; Electrophoresis, Polyacrylamide Gel; Guanidine; Muramidase; Protein Denaturation; Protein Folding; Protein Renaturation; Urea | 2005 |
The effect of stabilizers and denaturants on the cold denaturation temperatures of proteins and implications for freeze-drying.
Topics: Calorimetry, Differential Scanning; Cold Temperature; Drug Stability; Excipients; Freeze Drying; Glycerol; Guanidine; Hot Temperature; Hydrogen-Ion Concentration; Lactoglobulins; Phosphoglycerate Kinase; Protein Denaturation; Spectrometry, Fluorescence; Sucrose; Trehalose; Urea | 2005 |
Measurement of the kinetics of protein unfolding in viscous systems and implications for protein stability in freeze-drying.
Topics: Calorimetry, Differential Scanning; Cold Temperature; Drug Stability; Excipients; Freeze Drying; Guanidine; Kinetics; Lactoglobulins; Phosphoglycerate Kinase; Protein Denaturation; Spectrometry, Fluorescence; Sucrose; Trehalose; Urea; Viscosity | 2005 |
Biophysical characterization of insoluble aggregates of a multi-domain protein: an insight into the role of the various domains.
Topics: Calorimetry, Differential Scanning; Chemistry, Pharmaceutical; Drug Stability; Electrophoresis, Polyacrylamide Gel; Glycoproteins; Guanidine; Immunoglobulin G; Mercaptoethanol; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Temperature; Urea | 2005 |
Apparent pKa shifts of titratable residues at high denaturant concentration and the impact on protein stability.
Topics: Aspartic Acid; Chemical Phenomena; Chemistry, Physical; Glutamic Acid; Guanidine; Histidine; Hydrogen-Ion Concentration; Lysine; Magnetic Resonance Spectroscopy; Protein Folding; Proteins; Sensitivity and Specificity; Titrimetry; Urea | 2005 |
Purification and antigenicity of two recombinant forms of Boophilus microplus yolk pro-cathepsin expressed in inclusion bodies.
Topics: Animals; Aspartic Acid Endopeptidases; Blotting, Western; Cloning, Molecular; DNA, Complementary; Enzyme Precursors; Epitopes; Female; Gene Expression Regulation; Guanidine; Inclusion Bodies; Molecular Sequence Data; Recombinant Proteins; Sarcosine; Solubility; Ticks; Urea | 2006 |
Slow irreversible unfolding of Pyrococcus furiosus triosephosphate isomerase: separation and quantitation of conformers through a novel electrophoretic approach.
Topics: Circular Dichroism; Cold Temperature; Electrophoresis, Polyacrylamide Gel; Guanidine; Hot Temperature; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Pyrococcus furiosus; Sodium Dodecyl Sulfate; Triose-Phosphate Isomerase; Urea | 2005 |
Comparison of guanidine hydrochloride (GdnHCl) and urea denaturation on inactivation and unfolding of human placental cystatin (HPC).
Topics: Cystatins; Dose-Response Relationship, Drug; Guanidine; Humans; Placenta; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Urea | 2005 |
The pathway by which the tetrameric protein transthyretin dissociates.
Topics: Dimerization; Disulfides; Guanidine; Mutagenesis; Prealbumin; Protein Denaturation; Protein Structure, Quaternary; Protein Structure, Secondary; Urea | 2005 |
Improved 2-nitrobenzenesulfenyl method: optimization of the protocol and improved enrichment for labeled peptides.
Topics: Animals; Chickens; Guanidine; Mice; Nitrobenzenes; Peptides; Protein Denaturation; Proteomics; Sequence Analysis, Protein; Urea | 2006 |
Protein stabilization by specific binding of guanidinium to a functional arginine-binding surface on an SH3 domain.
Topics: Animals; Arginine; Binding Sites; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Mutation; Peptides; Protein Binding; Protein Denaturation; Proto-Oncogene Proteins c-fyn; Sodium Chloride; src Homology Domains; Surface Properties; Thermodynamics; Urea | 2006 |
Multiple unfolding states of glutathione transferase from Physa acuta (Gastropoda [correction of Gastropada]: Physidae).
Topics: Animals; Enzyme Stability; Gastropoda; Glutathione Transferase; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Urea | 2006 |
Chemical denaturation of the elongation factor 1alpha isolated from the hyperthermophilic archaeon Sulfolobus solfataricus.
Topics: Bacterial Proteins; Enzyme Stability; Guanidine; Hot Temperature; Peptide Elongation Factor 1; Protein Conformation; Protein Denaturation; Sulfolobus solfataricus; Thermodynamics; Urea | 2006 |
Biochemical properties and inhibitors of (N-)WASP.
Topics: Actin-Related Protein 2-3 Complex; Actins; Carbazoles; cdc42 GTP-Binding Protein; Fluorescence Resonance Energy Transfer; Guanidine; Humans; Nuclear Magnetic Resonance, Biomolecular; Propanolamines; Protein Denaturation; Protein Structure, Quaternary; Protein Structure, Tertiary; Pyrenes; Urea; Wiskott-Aldrich Syndrome Protein, Neuronal | 2006 |
Structural characterization of an equilibrium unfolding intermediate in cytochrome c.
Topics: Amino Acid Substitution; Animals; Cytochromes c; Guanidine; Horses; Protein Denaturation; Protein Folding; Spectrophotometry; Urea | 2006 |
Crystallization and preliminary characterization of a highly thermostable lectin from Trichosanthes dioica and comparison with other Trichosanthes lectins.
Topics: Crystallization; Crystallography, X-Ray; Guanidine; Hemagglutination; Hot Temperature; Hydrogen-Ion Concentration; Plant Lectins; Protein Denaturation; Protein Structure, Quaternary; Seeds; Trichosanthes; Urea | 2006 |
Characterization of intra-molecular distances and site-specific dynamics in chemically unfolded barstar: evidence for denaturant-dependent non-random structure.
Topics: Bacterial Proteins; Cysteine; Fluorescence Resonance Energy Transfer; Guanidine; Models, Molecular; Molecular Structure; Mutation; Nitrobenzoates; Protein Conformation; Protein Denaturation; Sulfhydryl Compounds; Urea | 2006 |
Matrix with high salt tolerance for the analysis of peptide and protein samples by desorption/ionization time-of-flight mass spectrometry.
Topics: Benzophenones; Cations; Guanidine; Insulin; Metals; Neurotensin; Peptides; Phenylenediamines; Proteins; Sensitivity and Specificity; Serum Albumin, Bovine; Solutions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin; Urea | 2006 |
Dissecting the role of protein-protein and protein-nucleic acid interactions in MS2 bacteriophage stability.
Topics: Anilino Naphthalenesulfonates; DNA, Viral; Fluorescent Dyes; Guanidine; Hot Temperature; Levivirus; Macromolecular Substances; Mutation; Nucleic Acid Conformation; Protein Conformation; Protein Denaturation; Urea; Viral Proteins | 2006 |
An evidence for the equilibrium unfolding intermediates of ribonuclease A by tritium labeling method.
Topics: Amino Acids; Guanidine; Hydrophobic and Hydrophilic Interactions; In Vitro Techniques; Lithium Chloride; Pancreas; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Ribonuclease, Pancreatic; Tritium; Urea | 2006 |
Conformational stability of secretory leucocyte protease inhibitor: a protein with no hydrophobic core and very little secondary structure.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithiothreitol; Guanidine; Guanidines; Humans; Hydrophobic and Hydrophilic Interactions; Isothiocyanates; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteinase Inhibitory Proteins, Secretory; Proteins; Recombinant Proteins; Secretory Leukocyte Peptidase Inhibitor; Thermodynamics; Urea | 2006 |
Urea-, thiourea-, and guanidine-linked glycooligomers as phosphate binders in water.
Topics: Carbohydrate Conformation; Carbohydrate Sequence; Guanidine; Molecular Sequence Data; Oligosaccharides; Phosphates; Stereoisomerism; Thiourea; Urea; Water | 2006 |
Effects of surface charge on denaturation of bovine carbonic anhydrase.
Topics: Acetylation; Animals; Carbonic Anhydrases; Cattle; Guanidine; Lysine; Models, Molecular; Osmolar Concentration; Protein Denaturation; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Static Electricity; Surface Properties; Temperature; Thermodynamics; Urea | 2006 |
NUB1 suppresses the formation of Lewy body-like inclusions by proteasomal degradation of synphilin-1.
Topics: Adaptor Proteins, Signal Transducing; alpha-Synuclein; Binding Sites; Brain; Carrier Proteins; Cells, Cultured; Down-Regulation; Gene Expression Profiling; Guanidine; Humans; Lewy Bodies; NEDD8 Protein; Nerve Tissue Proteins; Proteasome Endopeptidase Complex; Protein Binding; Protein Processing, Post-Translational; Protein Transport; RNA Interference; RNA, Messenger; Sodium Dodecyl Sulfate; Solubility; Solutions; Transcription Factors; Ubiquitins; Urea; Yeasts | 2006 |
The location of an engineered inter-subunit disulfide bond in factor for inversion stimulation (FIS) affects the denaturation pathway and cooperativity.
Topics: Circular Dichroism; Crystallography, X-Ray; Cysteine; Disulfides; DNA; Factor For Inversion Stimulation Protein; Fluorescence; Guanidine; Hydrogen Bonding; Hydrogen-Ion Concentration; Models, Molecular; Mutation; Oxidation-Reduction; Protein Binding; Protein Denaturation; Protein Engineering; Protein Subunits; Serine; Structure-Activity Relationship; Urea; Valine | 2006 |
Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii.
Topics: Cations, Divalent; Cloning, Molecular; Dimerization; Dimethyl Sulfoxide; Dithiothreitol; Enzyme Stability; Escherichia coli; Guanidine; Hexosaminidases; Hydrogen-Ion Concentration; Pyrococcus horikoshii; Recombinant Proteins; Sodium Dodecyl Sulfate; Temperature; Urea | 2006 |
Conformational stability of 17 beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus.
Topics: 17-Hydroxysteroid Dehydrogenases; Ascomycota; Circular Dichroism; Enzyme Stability; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Recombinant Proteins; Spectrophotometry, Ultraviolet; Temperature; Urea | 2006 |
Effect of denaturants on the structure and activity of 3-hydroxybenzoate-6-hydroxylase.
Topics: Bacteria; Circular Dichroism; Guanidine; Mixed Function Oxygenases; Protein Denaturation; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Urea | 2006 |
Physical nature of signal peptide binding to DmsD.
Topics: Binding Sites; Blotting, Western; Carrier Proteins; Chromatography, Gel; Dose-Response Relationship, Drug; Escherichia coli Proteins; Glutathione Transferase; Guanidine; Intracellular Signaling Peptides and Proteins; Protein Binding; Protein Denaturation; Protein Folding; Protein Sorting Signals; Recombinant Fusion Proteins; Thermodynamics; Urea | 2006 |
Key role of coulombic interactions for the folding transition state of the cold shock protein.
Topics: Bacterial Proteins; Binding Sites; Guanidine; Models, Molecular; Mutation; Protein Conformation; Protein Folding; Urea | 2006 |
Equilibrium unfolding of DLC8 monomer by urea and guanidine hydrochloride: Distinctive global and residue level features.
Topics: Carrier Proteins; Circular Dichroism; Drosophila Proteins; Dyneins; Guanidine; Magnetic Resonance Imaging; Protein Conformation; Protein Denaturation; Recombinant Proteins; Urea | 2007 |
A mass spectrometry-based probe of equilibrium intermediates in protein-folding reactions.
Topics: Animals; Cattle; Cytochromes c; Deuterium Oxide; Fatty Acid-Binding Proteins; Guanidine; Kinetics; Lactalbumin; Mass Spectrometry; Protein Folding; Thermodynamics; Urea | 2006 |
Multiple intermediate conformations of jack bean urease at low pH: anion-induced refolding.
Topics: Anions; Circular Dichroism; Fabaceae; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Urea; Urease | 2006 |
Denaturation of an extremely stable hyperthermophilic protein occurs via a dimeric intermediate.
Topics: Archaeal Proteins; beta-Glucosidase; Circular Dichroism; Cloning, Molecular; Dimerization; Enzyme Stability; Guanidine; Models, Molecular; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Subunits; Pyrococcus furiosus; Recombinant Proteins; Spectrometry, Fluorescence; Temperature; Urea | 2007 |
Identification and characterization of functional intermediates of stem bromelain during urea and guanidine hydrochloride unfolding.
Topics: Anilino Naphthalenesulfonates; Bromelains; Circular Dichroism; Ethanol; Fluorescence; Guanidine; Protein Conformation; Protein Denaturation; Protein Folding; Solvents; Structure-Activity Relationship; Urea | 2007 |
Characterization of a lectin from Gonatanthus pumilus D. Don having anti-proliferative effect against human cancer cell lines.
Topics: Antifungal Agents; Antineoplastic Agents; Cell Line, Tumor; Cell Proliferation; Guanidine; Humans; India; Neoplasms; Plant Lectins; Plants; Protein Denaturation; Spectrometry, Fluorescence; Urea | 2007 |
Guanidinium chloride and urea denaturations of beta-lactoglobulin A at pH 2.0 and 25 degrees C: the equilibrium intermediate contains non-native structures (helix, tryptophan and hydrophobic patches).
Topics: Animals; Cattle; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Kinetics; Lactoglobulins; Parasympathomimetics; Protein Denaturation; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 2007 |
Effect of ionic detergents, nonionic detergents, and chaotropic agents on polyphenol oxidase activity from dormant saffron (Crocus sativus L.) corms.
Topics: Anions; Catechol Oxidase; Crocus; Enzyme Activation; Enzyme Inhibitors; Guanidine; Surface-Active Agents; Urea | 2007 |
The unfolding pathway for Apo Escherichia coli aspartate aminotransferase is dependent on the choice of denaturant.
Topics: Apoenzymes; Aspartate Aminotransferases; Circular Dichroism; Escherichia coli; Guanidine; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Spectrometry, Fluorescence; Temperature; Urea | 2007 |
Structure and denaturation of 4-chlorobenzoyl coenzyme A dehalogenase from Arthrobacter sp. strain TM-1.
Topics: Amino Acid Sequence; Arthrobacter; Enzyme Stability; Guanidine; Hydrogen-Ion Concentration; Hydrolases; Molecular Sequence Data; Protein Denaturation; Protein Structure, Secondary; Sequence Alignment; Temperature; Urea | 2008 |
Cofactor-directed reversible denaturation pathways: the cofactor-stabilized Escherichia coli aspartate aminotransferase homodimer unfolds through a pathway that differs from that of the apoenzyme.
Topics: Aspartate Aminotransferases; Circular Dichroism; Enzyme Stability; Escherichia coli; Guanidine; Holoenzymes; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Quaternary; Pyridoxal Phosphate; Pyridoxamine; Spectrometry, Fluorescence; Thermodynamics; Urea | 2007 |
Purification and in vitro refolding of maize chloroplast transglutaminase over-expressed in Escherichia coli.
Topics: Biotechnology; Chloroplasts; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transglutaminases; Urea; Zea mays | 2007 |
Interactions between hydrophobic and ionic solutes in aqueous guanidinium chloride and urea solutions: lessons for protein denaturation mechanism.
Topics: Algorithms; Animals; Computer Simulation; Guanidine; Hydrogen Bonding; Hydrophobic and Hydrophilic Interactions; Ions; Mice; Protein Denaturation; Protein Structure, Secondary; Proteins; Solutions; Urea; Water | 2007 |
Virus inactivation by protein denaturants used in affinity chromatography.
Topics: Chromatography, Affinity; Drug Contamination; Ethanol; Guanidine; Guanidines; Herpesvirus 1, Human; Humans; Hydrogen-Ion Concentration; Poliovirus; Protein Denaturation; Sindbis Virus; Sodium Hydroxide; Thiocyanates; Urea; Vaccinia virus; Virus Inactivation | 2007 |
Comparison of digestion protocols for microgram quantities of enriched protein samples.
Topics: Animals; Bacterial Proteins; Cattle; Chromatography, Liquid; Guanidine; Microtubule-Associated Proteins; Organic Chemicals; Proteome; Rhodopseudomonas; Ribosomes; Tandem Mass Spectrometry; Trypsin; Urea | 2007 |
Accumulation of partly folded states in the equilibrium unfolding of ervatamin A: spectroscopic description of the native, intermediate, and unfolded states.
Topics: Biophysical Phenomena; Biophysics; Circular Dichroism; Cysteine Endopeptidases; Enzyme Stability; Guanidine; Hydrogen-Ion Concentration; Kinetics; Magnoliopsida; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Spectrometry, Fluorescence; Temperature; Thermodynamics; Ultraviolet Rays; Urea | 2007 |
Dynamic similarity of the unfolded states of proteins L and G.
Topics: Algorithms; Bacterial Proteins; Computer Simulation; Deuterium Exchange Measurement; DNA-Binding Proteins; Fluorescence Resonance Energy Transfer; Guanidine; Hydrogen Bonding; Hydrophobic and Hydrophilic Interactions; Models, Chemical; Models, Theoretical; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Proteins; Recombinant Fusion Proteins; Thermodynamics; Tryptophan; Urea | 2007 |
Trimethylamine N-oxide counteracts the denaturing effects of urea or GdnHCl on protein denatured state.
Topics: Dipeptides; Guanidine; Methylamines; Protein Conformation; Protein Denaturation; Protein Folding; Urea | 2007 |
Conformational plasticity of cryptolepain: accumulation of partially unfolded states in denaturants induced equilibrium unfolding.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Plant Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Temperature; Thermodynamics; Urea | 2007 |
A unique and differential effect of denaturants on cofactor mediated activation of Plasmodium falciparum beta-ketoacyl-ACP reductase.
Topics: 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; Alcohol Oxidoreductases; Animals; Chromatography, Gel; Circular Dichroism; Enzyme Activation; Guanidine; Plasmodium falciparum; Protein Denaturation; Spectrometry, Fluorescence; Urea | 2008 |
A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S).
Topics: Antigens, Plant; Circular Dichroism; Globulins; Guanidine; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Seed Storage Proteins; Soybean Proteins; Spectrometry, Fluorescence; Urea | 2007 |
Hydrogen peroxide affects abscisic acid binding to ABAP1 in barley aleurones.
Topics: Abscisic Acid; Binding Sites; Dose-Response Relationship, Drug; Guanidine; Hordeum; Hydrogen Peroxide; Kinetics; Plant Growth Regulators; Plant Proteins; Protein Conformation; Protein Folding; Time Factors; Urea | 2007 |
Cation and strong co-solute effects on protein kinetic stability.
Topics: Cations; Cesium; Glycerol; Guanidine; Kinetics; Luminescent Proteins; Protein Denaturation; Quaternary Ammonium Compounds; Red Fluorescent Protein; Salts; Solubility; Solutions; Sucrose; Urea | 2007 |
Peptide sequence and conformation strongly influence tryptophan fluorescence.
Topics: Acrylamides; Biophysics; Computational Biology; Guanidine; Iodides; Molecular Conformation; Normal Distribution; Peptides; Spectrometry, Fluorescence; Temperature; Time Factors; Tryptophan; Tyrosine; Urea | 2008 |
Coulomb forces control the density of the collapsed unfolded state of barstar.
Topics: Bacterial Proteins; Dose-Response Relationship, Drug; Escherichia coli; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Genetic Variation; Guanidine; Mutagenesis, Site-Directed; Plasmids; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Static Electricity; Urea | 2008 |
A new method for determining the constant-pressure heat capacity change associated with the protein denaturation induced by guanidinium chloride (or urea).
Topics: Calorimetry, Differential Scanning; Guanidine; Hot Temperature; Protein Denaturation; Proteins; Urea | 2008 |
A conformationally isoformic thermophilic protein with high kinetic unfolding barriers.
Topics: Alcohol Dehydrogenase; Cross-Linking Reagents; Dimerization; Guanidine; Isoenzymes; Kinetics; Models, Chemical; Models, Molecular; Prions; Protein Conformation; Protein Folding; Protein Renaturation; Saccharomyces cerevisiae; Thermoanaerobacter; Thermodynamics; Urea | 2008 |
Demonstration by burst-phase analysis of a robust folding intermediate in the FF domain.
Topics: Guanidine; Hydrogen-Ion Concentration; Mutation; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Tertiary; Proteins; Temperature; Thermodynamics; Urea | 2008 |
Urea and guanidinium chloride denature protein L in different ways in molecular dynamics simulations.
Topics: Bacterial Proteins; Binding Sites; Computer Simulation; Guanidine; Models, Chemical; Models, Molecular; Protein Binding; Protein Conformation; Protein Denaturation; Urea | 2008 |
Bromophenol blue binding as a probe to study urea and guanidine hydrochloride denaturation of bovine serum albumin.
Topics: Animals; Bromphenol Blue; Cattle; Guanidine; Indicators and Reagents; Protein Denaturation; Serum Albumin, Bovine; Spectrophotometry; Urea | 2008 |
Use of protein folding reagents.
Topics: Dithiothreitol; Glutathione; Guanidine; Mercaptoethanol; Oxidants; Protein Denaturation; Protein Folding; Reducing Agents; Sulfhydryl Reagents; Urea | 2001 |
Enzymatic digestion of proteins in solution.
Topics: Alkylation; Amino Acid Sequence; Biochemistry; Guanidine; Molecular Sequence Data; Oxidation-Reduction; Peptide Hydrolases; Peptides; Protein Denaturation; Proteins; Sodium Dodecyl Sulfate; Solutions; Substrate Specificity; Urea | 2001 |
Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli.
Topics: Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Recombinant Proteins; Solubility; Urea | 2004 |
Assessment of the stability and unfolding pathways of azurin from Pseudomonas aeruginosa through the combination of denaturating osmolytes.
Topics: Azurin; Guanidine; Kinetics; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Urea | 2008 |
Effect of linkage geometry on biological activity in thiourea- and guanidine-substituted acridines and platinum-acridines.
Topics: Acridines; Antineoplastic Agents; Cell Line, Tumor; Chemistry, Pharmaceutical; Drug Design; Drug Resistance, Neoplasm; Drug Screening Assays, Antitumor; Guanidine; HL-60 Cells; Humans; Inhibitory Concentration 50; Models, Chemical; Platinum; Structure-Activity Relationship; Thiourea; Urea | 2008 |
Arginine increases the solubility of coumarin: comparison with salting-in and salting-out additives.
Topics: Arginine; Benzyl Alcohols; Biochemistry; Caffeine; Coumarins; Dose-Response Relationship, Drug; Guanidine; Hydrogen-Ion Concentration; Models, Biological; Salts; Solubility; Surface Properties; Urea; Water | 2008 |
Thermodynamic characterization of nucleoplasmin unfolding: interplay between function and stability.
Topics: Animals; Chromatography, Gel; Circular Dichroism; Female; Guanidine; Kinetics; Mutation; Nuclear Proteins; Nucleoplasmins; Phosphoproteins; Phosphorylation; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Ultracentrifugation; Urea; Xenopus laevis; Xenopus Proteins | 2008 |
Mechanism of transition from xanthine dehydrogenase to xanthine oxidase: effect of guanidine-HCL or urea on the activity.
Topics: Animals; Cattle; Guanidine; Liver; Milk; Mutant Proteins; Mutation; NAD; Oxidation-Reduction; Rats; Urea; Xanthine Dehydrogenase; Xanthine Oxidase | 2008 |
Increased folding stability of TEM-1 beta-lactamase by in vitro selection.
Topics: Amino Acid Substitution; Base Sequence; beta-Lactamases; Crystallography, X-Ray; Directed Molecular Evolution; Disulfides; DNA Primers; Enzyme Stability; Escherichia coli; Guanidine; Models, Molecular; Molecular Sequence Data; Mutagenesis; Mutagenesis, Site-Directed; Peptide Library; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Selection, Genetic; Thermodynamics; Urea | 2008 |
Chemical denaturants inhibit the onset of dewetting.
Topics: Guanidine; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Protein Denaturation; Solutions; Urea; Water; Wettability | 2008 |
Methanocaldococcus jannaschii adenylosuccinate synthetase: studies on temperature dependence of catalytic activity and structural stability.
Topics: Adenylosuccinate Synthase; Enzyme Stability; Guanidine; Hot Temperature; Kinetics; Methanococcaceae; Protein Denaturation; Protein Folding; Structure-Activity Relationship; Urea | 2008 |
Solution structure and refolding of the Mycobacterium tuberculosis pentapeptide repeat protein MfpA.
Topics: Amino Acid Sequence; Bacterial Proteins; Circular Dichroism; DNA Topoisomerases, Type I; Drug Resistance, Microbial; Guanidine; Molecular Sequence Data; Monomeric GTP-Binding Proteins; Mycobacterium tuberculosis; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Urea | 2008 |
Comparison of NMR structural and dynamics features of the urea and guanidine-denatured states of GED.
Topics: Circular Dichroism; GTP Phosphohydrolases; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Ultraviolet Rays; Urea | 2009 |
Heterotrimeric coiled coils with core residue urea side chains.
Topics: Amino Acid Sequence; Carboxylic Acids; Chromatography, High Pressure Liquid; Circular Dichroism; Guanidine; Heterocyclic Compounds, 3-Ring; Indicators and Reagents; Models, Molecular; Molecular Sequence Data; Oligopeptides; Protein Conformation; Urea | 2008 |
Consequences of domain insertion on the stability and folding mechanism of a protein.
Topics: Calorimetry; Escherichia coli Proteins; Guanidine; Kinetics; Protein Folding; Protein Stability; Protein Structure, Tertiary; Proteins; Urea | 2009 |
Urea, but not guanidinium, destabilizes proteins by forming hydrogen bonds to the peptide group.
Topics: Guanidine; Hydrogen Bonding; Peptides; Protein Denaturation; Proteins; Thermodynamics; Urea | 2009 |
Investigation of cosolute-protein preferential interaction coefficients: new insight into the mechanism by which arginine inhibits aggregation.
Topics: Arginine; Chymotrypsinogen; Glucose; Glycerol; Guanidine; Muramidase; Osmolar Concentration; Particle Size; Pressure; Serum Albumin, Bovine; Solutions; Urea; Volatilization | 2009 |
Kinetics of contact formation and end-to-end distance distributions of swollen disordered peptides.
Topics: Algorithms; Buffers; Circular Dichroism; Cysteine; Energy Transfer; Guanidine; Kinetics; Models, Chemical; Peptides; Protein Conformation; Protein Denaturation; Tryptophan; Urea | 2009 |
Cooperative effects of urea and L-arginine on protein refolding.
Topics: Arginine; Granulocyte Colony-Stimulating Factor; Guanidine; Humans; Protein Conformation; Protein Folding; Recombinant Proteins; Urea | 2009 |
Guanidine hydrochloride- and urea-induced unfolding of Toxoplasma gondii ferredoxin-NADP+ reductase: stabilization of a functionally inactive holo-intermediate.
Topics: Animals; Circular Dichroism; Ferredoxin-NADP Reductase; Guanidine; Protein Conformation; Protein Folding; Spectrometry, Fluorescence; Temperature; Toxoplasma; Urea | 2009 |
Influence of surface groups of proteins on water studied by freezing/thawing hysteresis and infrared spectroscopy.
Topics: Ammonium Chloride; Animals; Antifreeze Proteins; Cattle; Cytochromes c; Flounder; Freezing; Glutamic Acid; Guanidine; Methanol; Methylamines; Peptides; Polylysine; Proteins; Serum Albumin, Bovine; Sodium Acetate; Spectrophotometry, Infrared; Temperature; Transition Temperature; Urea; Water | 2009 |
Biophysical characterization and unfolding of LEF4 factor of RNA polymerase from AcNPV.
Topics: Acrylamide; Adenosine Triphosphatases; Amino Acid Sequence; Biophysical Phenomena; Circular Dichroism; DNA-Directed RNA Polymerases; Electrophoresis, Polyacrylamide Gel; Guanidine; Molecular Sequence Data; Nucleopolyhedroviruses; Protein Denaturation; Protein Folding; Recombinant Proteins; Temperature; Urea; Viral Proteins | 2009 |
Conformational stability of alpha-amylase from malted sorghum (Sorghum bicolor): reversible unfolding by denaturants.
Topics: alpha-Amylases; Enzyme Stability; Guanidine; Plants; Protein Folding; Sorghum; Temperature; Urea | 2009 |
Enhanced prion protein stability coupled to DNA recognition and milieu acidification.
Topics: Animals; DNA; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Mice; Prions; Protein Denaturation; Protein Folding; Protein Stability; Recombinant Proteins; Salts; Spectrum Analysis; Urea | 2009 |
Hsp70-1 from Plasmodium falciparum: protein stability, domain analysis and chaperone activity.
Topics: Animals; Circular Dichroism; Cloning, Molecular; Dose-Response Relationship, Drug; Guanidine; HSP72 Heat-Shock Proteins; Molecular Chaperones; Plasmodium falciparum; Polymerase Chain Reaction; Protein Stability; Protein Structure, Tertiary; Spectrometry, Fluorescence; Temperature; Urea | 2009 |
Stability check of succinylated concanavalin A: presence of functionally active conformational state.
Topics: Concanavalin A; Guanidine; Hot Temperature; Protein Conformation; Protein Denaturation; Protein Folding; Protein Multimerization; Protein Stability; Spectrometry, Fluorescence; Urea | 2009 |
Revealing a concealed intermediate that forms after the rate-limiting step of refolding of the SH3 domain of PI3 kinase.
Topics: Guanidine; Hydrogen-Ion Concentration; Kinetics; Phosphatidylinositol 3-Kinases; Protein Denaturation; Protein Folding; src Homology Domains; Thermodynamics; Urea | 2009 |
Refolding of ribonuclease A monitored by real-time photo-CIDNP NMR spectroscopy.
Topics: Animals; Cattle; Guanidine; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Photochemistry; Protein Denaturation; Protein Folding; Ribonuclease, Pancreatic; Urea | 2009 |
Conformational stability and multistate unfolding of poly(A)-specific ribonuclease.
Topics: Guanidine; Poly A; Protein Conformation; Protein Denaturation; Ribonucleases; Static Electricity; Urea | 2009 |
Structural, functional and unfolding characteristics of glutathione S-transferase of Plasmodium vivax.
Topics: Animals; Enzyme Stability; Glutathione; Glutathione Transferase; Guanidine; Models, Molecular; Plasmodium vivax; Protein Denaturation; Protein Multimerization; Protein Structure, Quaternary; Salts; Solvents; Spectrum Analysis; Urea | 2009 |
Complete conformational stability of kinetically stable dimeric serine protease milin against pH, temperature, urea, and proteolysis.
Topics: Animals; Calorimetry, Differential Scanning; Enzyme Stability; Euphorbia; Guanidine; Hydrogen-Ion Concentration; Kinetics; Protein Denaturation; Protein Multimerization; Protein Structure, Quaternary; Serine Proteases; Temperature; Urea | 2009 |
Conformational stability of esterase enzymes from different sources.
Topics: Alicyclobacillus; Archaeal Proteins; Archaeoglobales; Bacterial Proteins; Carboxylic Ester Hydrolases; Catalytic Domain; Circular Dichroism; Crystallography, X-Ray; Enzyme Stability; Escherichia coli; Esterases; Guanidine; Kinetics; Mutation; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Sterol Esterase; Temperature; Urea | 2009 |
Computing protein stabilities from their chain lengths.
Topics: Algorithms; Computational Biology; Computer Simulation; Entropy; Guanidine; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Protein Stability; Proteins; Sodium Chloride; Static Electricity; Temperature; Thermodynamics; Urea | 2009 |
Leishmania donovani trypanothione reductase: role of urea and guanidine hydrochloride in modulation of functional and structural properties.
Topics: Animals; Circular Dichroism; Dimerization; Enzyme Stability; Flavin-Adenine Dinucleotide; Guanidine; Leishmania donovani; Models, Molecular; NADH, NADPH Oxidoreductases; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Spectrometry, Fluorescence; Spectrophotometry; Sulfhydryl Compounds; Thermodynamics; Tryptophan; Urea | 2009 |
Combinatorial peptide ligand libraries for urine proteome analysis: investigation of different elution systems.
Topics: Adsorption; Adult; Chemical Precipitation; Cholic Acids; Citric Acid; Combinatorial Chemistry Techniques; Electrophoresis, Gel, Two-Dimensional; Female; Guanidine; Humans; Hydrogen-Ion Concentration; Male; Peptide Library; Proteinuria; Proteome; Sodium Dodecyl Sulfate; Thiourea; Urea | 2009 |
Synthesis of new linear guanidines and macrocyclic amidinourea derivatives endowed with high antifungal activity against Candida spp. and Aspergillus spp.
Topics: Antifungal Agents; Aspergillus; Candida; Drug Design; Guanidine; Humans; Macrocyclic Compounds; Microbial Sensitivity Tests; Urea | 2009 |
Sequential dissociation of subunits from bovine heart cytochrome C oxidase by urea.
Topics: Animals; Cattle; Dose-Response Relationship, Drug; Electron Transport Complex IV; Guanidine; Humans; Myocardium; Protein Denaturation; Reproducibility of Results; Thermodynamics; Urea | 2009 |
Binding of 6-propyl-2-thiouracil to human serum albumin destabilized by chemical denaturants.
Topics: Antithyroid Agents; Binding Sites; Guanidine; Humans; Propylthiouracil; Protein Binding; Protein Denaturation; Serum Albumin; Spectrometry, Fluorescence; Urea | 2009 |
Guanidine hydrochloride and urea-induced unfolding of Brugia malayi hexokinase.
Topics: Animals; Brugia malayi; Chromatography, Gel; Circular Dichroism; Glutaral; Guanidine; Helminth Proteins; Hexokinase; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Protein Multimerization; Spectrometry, Fluorescence; Urea | 2010 |
Denaturant-dependent conformational changes in a beta-trefoil protein: global and residue-specific aspects of an equilibrium denaturation process.
Topics: Circular Dichroism; Endopeptidase K; Fluorescence Polarization; Guanidine; Humans; Interleukin 1 Receptor Antagonist Protein; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Protein Conformation; Protein Denaturation; Recombinant Proteins; Scattering, Radiation; Spectrometry, Fluorescence; Thermodynamics; Urea; X-Rays | 2009 |
Influences of urea and guanidine hydrochloride on the interaction of 6-thioguanine with bovine serum albumin.
Topics: Animals; Buffers; Cattle; Guanidine; Hydrogen-Ion Concentration; Kinetics; Protein Denaturation; Serum Albumin, Bovine; Spectrometry, Fluorescence; Thioguanine; Urea | 2009 |
Refolding of an unstable lysozyme by gradient removal of a solubilizer and gradient addition of a stabilizer.
Topics: Animals; Chickens; Dialysis; Enzyme Stability; Glycerol; Guanidine; Muramidase; Oxidation-Reduction; Protein Denaturation; Protein Folding; Solubility; Temperature; Urea | 2010 |
Structure and activity of lysozyme on binding to ZnO nanoparticles.
Topics: Anilino Naphthalenesulfonates; Animals; Calorimetry; Catalytic Domain; Cross-Linking Reagents; Glutaral; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Muramidase; Nanoparticles; Particle Size; Protein Denaturation; Protein Multimerization; Protein Structure, Quaternary; Protein Structure, Secondary; Spectrometry, Fluorescence; Thermodynamics; Urea; Zinc Oxide | 2010 |
Denaturation and solvent effect on the conformation and fibril formation of TGFBIp.
Topics: Amyloid; Cell Line; Extracellular Matrix Proteins; Guanidine; Humans; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Solvents; Spectrometry, Fluorescence; Transforming Growth Factor beta; Trifluoroethanol; Tryptophan; Urea | 2009 |
A capillary monolithic trypsin reactor for efficient protein digestion in online and offline coupling to ESI and MALDI mass spectrometry.
Topics: Acetonitriles; Analytic Sample Preparation Methods; Animals; Cattle; Cytochromes c; Enzymes, Immobilized; Guanidine; Humans; Muramidase; Online Systems; Polymers; Protein Denaturation; Proteins; Serum Albumin, Bovine; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin; Urea | 2010 |
The amidine-pentacyanoferrate reaction; a test for guanidines, urea and thiourea.
Topics: Acids; Amidines; Guanidine; Guanidines; Thiourea; Urea | 1946 |
Circulatory properties of iso-thioureas, guanidines, iso-ureas and amidines.
Topics: Amidines; Blood Pressure; Guanidine; Guanidines; Thiourea; Urea; Vasomotor System | 1947 |
Differential scanning calorimetry and fluorescence study of lactoperoxidase as a function of guanidinium-HCl, urea, and pH.
Topics: Animals; Calorimetry; Calorimetry, Differential Scanning; Cattle; Guanidine; Hydrochloric Acid; Hydrogen-Ion Concentration; Kinetics; Lactoperoxidase; Microscopy, Fluorescence; Molecular Conformation; Temperature; Thermodynamics; Tryptophan; Urea | 2010 |
Increasing protein stability: importance of DeltaC(p) and the denatured state.
Topics: Bacteria; Circular Dichroism; Escherichia coli; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Protein Stability; Recombinant Proteins; Ribonucleases; Sodium Chloride; Thermodynamics; Urea | 2010 |
Study of conformational changes in glucoamylase of Aspergillus awamori nakazawa in presence of denaturants through CD-spectroscopy.
Topics: Aspergillus; Circular Dichroism; Glucan 1,4-alpha-Glucosidase; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Protein Structure, Secondary; Urea | 2010 |
Physicochemical properties of thiol proteinase inhibitor isolated from goat pancreas.
Topics: Anilino Naphthalenesulfonates; Animals; Chemical Phenomena; Circular Dichroism; Cystatins; Dimerization; Fluorescent Dyes; Goats; Guanidine; In Vitro Techniques; Pancreas; Protein Denaturation; Protein Structure, Secondary; Spectrometry, Fluorescence; Urea | 2010 |
Deglycosylated milin unfolds via inactive monomeric intermediates.
Topics: Circular Dichroism; Enzyme Stability; Glycosides; Guanidine; Hydrogen-Ion Concentration; Kinetics; Mesylates; Protein Folding; Protein Structure, Secondary; Serine Proteases; Solubility; Spectrometry, Fluorescence; Urea | 2010 |
Characterization of membrane protein non-native states. 1. Extent of unfolding and aggregation of rhodopsin in the presence of chemical denaturants.
Topics: Animals; Cattle; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Guanidine; Hydrophobic and Hydrophilic Interactions; Magnetic Resonance Spectroscopy; Membrane Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Protein Multimerization; Rhodopsin; Sodium Dodecyl Sulfate; Trifluoroacetic Acid; Urea | 2010 |
Characterization of functional intermediates of endoglucanase from Aspergillus aculeatus during urea and guanidine hydrochloride unfolding.
Topics: Aspergillus; Catalytic Domain; Cellulase; Guanidine; Kinetics; Protein Denaturation; Spectrometry, Fluorescence; Thermodynamics; Urea | 2010 |
Mechanisms of amphipathic helical peptide denaturation by guanidinium chloride and urea: a molecular dynamics simulation study.
Topics: Amino Acid Sequence; Guanidine; Molecular Dynamics Simulation; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Structure, Secondary; Urea | 2010 |
Concentration regimes and denaturation effects on the conformational changes of α-chymotrypsin by viscosity and dynamic light scattering measurements.
Topics: Acetonitriles; Animals; Cattle; Chemistry Techniques, Analytical; Chymotrypsin; Guanidine; Hydrodynamics; Light; Protein Conformation; Protein Denaturation; Scattering, Radiation; Solvents; Temperature; Urea; Viscosity | 2010 |
A quantitative study of the effects of chaotropic agents, surfactants, and solvents on the digestion efficiency of human plasma proteins by trypsin.
Topics: Acetonitriles; Biocatalysis; Blood Proteins; Deoxycholic Acid; Guanidine; Humans; Hydrolysis; Methanol; Peptides; Proteomics; Reproducibility of Results; Sodium Dodecyl Sulfate; Tandem Mass Spectrometry; Trifluoroethanol; Trypsin; Urea | 2010 |
Influence of urea and guanidine hydrochloride on lysozyme stability and thermal denaturation; a correlation between activity, protein dynamics and conformational changes.
Topics: Calorimetry, Differential Scanning; Guanidine; Muramidase; Protein Conformation; Protein Denaturation; Protein Stability; Spectrum Analysis, Raman; Temperature; Urea | 2010 |
The effect of zinc oxide nanoparticles on the structure of the periplasmic domain of the Vibrio cholerae ToxR protein.
Topics: Adsorption; Bacterial Proteins; Circular Dichroism; DNA-Binding Proteins; Guanidine; Nanoparticles; Periplasm; Protein Conformation; Protein Denaturation; Thermodynamics; Transcription Factors; Urea; Zinc Oxide | 2010 |
Xylanase II from Trichoderma reesei QM 9414: conformational and catalytic stability to Chaotropes, Trifluoroethanol, and pH changes.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Endo-1,4-beta Xylanases; Fluorescent Dyes; Fungal Proteins; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Trichoderma; Trifluoroethanol; Urea | 2011 |
The combination of fatty acids and related compounds with serum albumin; stabilization against urea and guanidine denaturation.
Topics: Fatty Acids; Guanidine; Guanidines; Humans; Proteins; Serum Albumin; Urea | 1946 |
The effect of riboflavin deficiency upon carbohydrate metabolism in anoxia.
Topics: Blood Proteins; Carbohydrate Metabolism; Fatty Acids; Guanidine; Guanidines; Proteins; Riboflavin Deficiency; Urea | 1946 |
Biophysical characterization of a recombinant α-amylase from thermophilic Bacillus sp. strain TS-23.
Topics: alpha-Amylases; Bacillus; Bacterial Proteins; Circular Dichroism; Guanidine; Protein Denaturation; Protein Stability; Protein Structure, Quaternary; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Ultracentrifugation; Urea | 2010 |
Stability profiles of nepenthesin in urea and guanidine hydrochloride: comparison with porcine pepsin A.
Topics: Animals; Aspartic Acid Endopeptidases; Enzyme Stability; Guanidine; Hydrogen-Ion Concentration; Pepsin A; Plant Proteins; Protein Denaturation; Sarraceniaceae; Swine; Temperature; Urea | 2010 |
Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin.
Topics: Adenylate Cyclase Toxin; Bordetella pertussis; Calcium; Circular Dichroism; Fluorescence; Guanidine; Models, Molecular; Protein Folding; Protein Stability; Protein Structure, Tertiary; Sodium Chloride; Spectroscopy, Fourier Transform Infrared; Tryptophan; Urea | 2010 |
Differences in the pathways of proteins unfolding induced by urea and guanidine hydrochloride: molten globule state and aggregates.
Topics: Actins; Anilino Naphthalenesulfonates; Animals; Carbonic Anhydrase II; Cattle; Fluorescent Dyes; Guanidine; Hydrophobic and Hydrophilic Interactions; Kinetics; Muscle, Skeletal; Protein Conformation; Protein Denaturation; Protein Unfolding; Proteins; Rabbits; Spectrometry, Fluorescence; Urea | 2010 |
Biophysical characterization of Entamoeba histolytica phosphoserine aminotransferase (EhPSAT): role of cofactor and domains in stability and subunit assembly.
Topics: Amino Acid Sequence; Apoenzymes; Biophysical Phenomena; Coenzymes; Entamoeba histolytica; Enzyme Stability; Guanidine; Holoenzymes; Models, Molecular; Molecular Sequence Data; Protein Denaturation; Protein Multimerization; Protein Structure, Quaternary; Protein Structure, Tertiary; Protein Subunits; Pyridoxal Phosphate; Transaminases; Urea | 2011 |
Protein unfolding studies of thiol-proteinase inhibitor from goat (Capra hircus) muscle in the presence of urea and GdnHCl as denaturants.
Topics: Anilino Naphthalenesulfonates; Animals; Cystatins; Goats; Guanidine; Muscles; Protease Inhibitors; Protein Denaturation; Protein Structure, Secondary; Protein Unfolding; Spectrometry, Fluorescence; Urea | 2011 |
Effects of urea and guanidine hydrochloride on the sliding movement of actin filaments with ATP hydrolysis by myosin molecules.
Topics: Actins; Adenosine Triphosphatases; Adenosine Triphosphate; Biocatalysis; Dose-Response Relationship, Drug; Enzyme Activation; Guanidine; Hydrogen Bonding; Hydrolysis; Myosins; Spectrometry, Fluorescence; Urea | 2011 |
Polyhydroxylated bicyclic isoureas and guanidines are potent glucocerebrosidase inhibitors and nanomolar enzyme activity enhancers in Gaucher cells.
Topics: Bridged Bicyclo Compounds; Cell Line; Enzyme Inhibitors; Enzyme Stability; Fibroblasts; Gaucher Disease; Glucosylceramidase; Guanidine; Humans; Hydroxylation; Mutation; Protein Denaturation; Temperature; Urea | 2011 |
The CLN025 decapeptide retains a β-hairpin conformation in urea and guanidinium chloride.
Topics: Circular Dichroism; Crystallography, X-Ray; Guanidine; Models, Molecular; Molecular Dynamics Simulation; Oligopeptides; Protein Conformation; Urea | 2011 |
Solvent denaturation of proteins and interpretations of the m value.
Topics: Animals; Guanidine; Humans; Linear Models; Protein Conformation; Protein Denaturation; Protein Stability; Proteins; Ribonuclease, Pancreatic; Ribonucleases; Thermodynamics; Urea | 2009 |
Equilibrium unfolding of A. niger RNase: pH dependence of chemical and thermal denaturation.
Topics: Aspergillus niger; Circular Dichroism; Endoribonucleases; Guanidine; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Protein Denaturation; Protein Unfolding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Temperature; Thermodynamics; Urea | 2011 |
Urea and guanidinium induced denaturation of a Trp-cage miniprotein.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Magnetic Resonance Spectroscopy; Models, Molecular; Protein Denaturation; Tryptophan; Urea | 2011 |
Structural stability as a probe for molecular evolution of homologous albumins studied by spectroscopy and bioinformatics.
Topics: Amino Acid Sequence; Animals; Cattle; Computational Biology; Dose-Response Relationship, Drug; Evolution, Molecular; Guanidine; Humans; Models, Molecular; Molecular Sequence Data; Protein Stability; Protein Structure, Tertiary; Protein Unfolding; Rabbits; Sequence Homology, Amino Acid; Serum Albumin; Spectrum Analysis; Static Electricity; Thermodynamics; Urea | 2011 |
Biophysical studies of an NAD(P)(+)-dependent aldehyde dehydrogenase from Bacillus licheniformis.
Topics: Aldehyde Dehydrogenase; Bacillus; Biophysical Phenomena; Circular Dichroism; Guanidine; Protein Multimerization; Protein Structure, Quaternary; Protein Unfolding; Sodium Dodecyl Sulfate; Solvents; Spectrometry, Fluorescence; Temperature; Tryptophan; Ultracentrifugation; Urea | 2011 |
Microsecond unfolding kinetics of sheep prion protein reveals an intermediate that correlates with susceptibility to classical scrapie.
Topics: Animals; Disease Susceptibility; Guanidine; Humans; Hydrogen-Ion Concentration; Kinetics; Models, Biological; Models, Molecular; Prions; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Unfolding; Scrapie; Sheep; Spectrometry, Fluorescence; Tryptophan; Urea | 2011 |
Influence of denaturants on native-state structural fluctuations in azurin probed by molecular size-dependent quenching of Trp phosphorescence.
Topics: Azurin; Guanidine; Protein Denaturation; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 2011 |
Residual transglutaminase in collagen - effects, detection, quantification, and removal.
Topics: Animals; Blotting, Western; Collagen Type I; Dialysis; Enzyme-Linked Immunosorbent Assay; Guanidine; Hydrogen-Ion Concentration; Sodium Chloride; Streptomyces; Swine; Transglutaminases; Urea | 2012 |
How does sucrose stabilize the native state of globular proteins?
Topics: Carbohydrates; Cold Temperature; Guanidine; Macromolecular Substances; Protein Conformation; Protein Denaturation; Proteins; Solvents; Sucrose; Temperature; Thermodynamics; Trehalose; Urea; Water | 2012 |
Dissociation of hydrophobic and charged nano particles in aqueous guanidinium chloride and urea solutions: a molecular dynamics study.
Topics: Amino Acids; Guanidine; Hydrogen Bonding; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Nanoparticles; Proteins; Urea; Water | 2012 |
Thermostable hexameric form of Eis (Rv2416c) protein of M. tuberculosis plays an important role for enhanced intracellular survival within macrophages.
Topics: Acetyl Coenzyme A; Acetyltransferases; Antigens, Bacterial; Bacterial Proteins; Cell Line, Tumor; Guanidine; Humans; Intracellular Space; Macrophages; Microbial Viability; Mycobacterium smegmatis; Mycobacterium tuberculosis; Protein Multimerization; Protein Stability; Protein Structure, Quaternary; Protein Unfolding; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Temperature; Urea | 2011 |
Molecular characterization of a novel trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformis.
Topics: Amino Acid Sequence; Bacillus; Cloning, Molecular; Computational Biology; Disaccharidases; Enzyme Stability; Escherichia coli; Genetic Vectors; Guanidine; Molecular Sequence Data; Molecular Weight; Promoter Regions, Genetic; Protein Denaturation; Sodium Dodecyl Sulfate; Solvents; Temperature; Urea; Water | 2012 |
Small-angle X-ray scattering and single-molecule FRET spectroscopy produce highly divergent views of the low-denaturant unfolded state.
Topics: Fluorescence Resonance Energy Transfer; Guanidine; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Unfolding; Proteins; Scattering, Small Angle; Urea; X-Rays | 2012 |
Folding studies on muscle type of creatine kinase from Pelodiscus sinensis.
Topics: Animals; Creatine Kinase, MM Form; Enzyme Activation; Guanidine; Kinetics; Protein Folding; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Turtles; Urea | 2012 |
Effect of cosolvents on nano-confined water: a molecular dynamics study.
Topics: Guanidine; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Nanotechnology; Nanotubes, Carbon; Solvents; Urea; Water | 2012 |
Differing structural characteristics of molten globule intermediate of peanut lectin in urea and guanidine-HCl.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Guanidine; Peanut Agglutinin; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Protein Unfolding; Temperature; Tryptophan; Urea | 2012 |
Length of polyglutamine tract affects secondary and tertiary structures of huntingtin protein.
Topics: Guanidine; Nerve Tissue Proteins; Peptide Fragments; Peptides; Protein Denaturation; Protein Refolding; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Urea | 2012 |
Stability of early-stage amyloid-β(1-42) aggregation species.
Topics: Amyloid; Amyloid beta-Peptides; Antibodies; Guanidine; Humans; Peptide Fragments; Protein Stability; Sodium Dodecyl Sulfate; Urea | 2013 |
A kinetic analysis of the folding and unfolding of OmpA in urea and guanidinium chloride: single and parallel pathways.
Topics: Bacterial Outer Membrane Proteins; Escherichia coli Proteins; Guanidine; Kinetics; Maltose; Protein Denaturation; Protein Folding; Surface-Active Agents; Urea | 2012 |
Solute's perspective on how trimethylamine oxide, urea, and guanidine hydrochloride affect water's hydrogen bonding ability.
Topics: Guanidine; Hydrogen Bonding; Methylamines; Thermodynamics; Urea; Water | 2012 |
Collapse of unfolded proteins in a mixture of denaturants.
Topics: Animals; Bacterial Proteins; Chickens; DNA-Binding Proteins; Guanidine; Models, Molecular; Muramidase; Peptococcus; Protein Conformation; Protein Unfolding; Urea | 2012 |
Structural comparison of ColH and ColG collagen-binding domains from Clostridium histolyticum.
Topics: Calcium; Calorimetry, Differential Scanning; Cations, Divalent; Clostridium histolyticum; Collagenases; Crystallography, X-Ray; Guanidine; Models, Molecular; Protein Binding; Protein Conformation; Protein Stability; Scattering, Small Angle; Sequence Homology, Amino Acid; Temperature; Urea | 2013 |
Thermal, chemical and pH induced denaturation of a multimeric β-galactosidase reveals multiple unfolding pathways.
Topics: beta-Galactosidase; Cicer; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Plant Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Protein Multimerization; Protein Stability; Protein Unfolding; Spectrometry, Fluorescence; Temperature; Thermodynamics; Urea | 2012 |
Using guanidine-hydrochloride for fast and efficient protein digestion and single-step affinity-purification mass spectrometry.
Topics: Chromatography, Affinity; Guanidine; High-Throughput Screening Assays; Mass Spectrometry; Metalloendopeptidases; Protein Interaction Mapping; Proteolysis; Proteomics; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Urea | 2013 |
Use of anionic denaturing detergents to purify insoluble proteins after overexpression.
Topics: Anions; Chromatography, Affinity; Detergents; Escherichia coli; Guanidine; Histidine; Inclusion Bodies; Oligopeptides; Protein Denaturation; Protein Refolding; Recombinant Fusion Proteins; Sodium Dodecyl Sulfate; Urea | 2012 |
Pharmacophoric features of drugs with guanylurea moiety: an electronic structure analysis.
Topics: Adrenergic alpha-2 Receptor Agonists; Electrons; Guanidine; Humans; Imidazoles; Kinetics; Models, Chemical; Molecular Docking Simulation; Phenylurea Compounds; Quantum Theory; Receptors, Adrenergic, alpha-2; Stereoisomerism; Structure-Activity Relationship; Thermodynamics; Urea | 2013 |
Denaturation-resistant bifunctional colloidal superstructures assembled via the proteinaceous barnase-barstar interface.
Topics: Animals; Bacterial Proteins; Colloids; Dose-Response Relationship, Drug; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Stability; Ribonucleases; Salts; Temperature; Urea | 2013 |
Striking stabilization of Rana catesbeiana ribonuclease 3 by guanidine hydrochloride.
Topics: Amphibian Proteins; Animals; Enzyme Stability; Escherichia coli; Gene Expression; Guanidine; Kinetics; Molecular Docking Simulation; Protein Denaturation; Protein Unfolding; Rana catesbeiana; Recombinant Proteins; Ribonuclease III; Ribonuclease, Pancreatic; Ribonucleases; Thermodynamics; Urea | 2013 |
Collapse of a hydrophobic polymer in a mixture of denaturants.
Topics: Guanidine; Hydrophobic and Hydrophilic Interactions; Polymers; Protein Denaturation; Urea | 2013 |
At very low concentrations known chaotropes act as kosmotropes for the N and B isoforms of human serum albumin.
Topics: Detergents; Guanidine; Humans; Hydrogen-Ion Concentration; Micelles; Models, Molecular; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Serum Albumin; Solutions; Urea | 2013 |
TK1656, a thermostable l-asparaginase from Thermococcus kodakaraensis, exhibiting highest ever reported enzyme activity.
Topics: Amino Acid Sequence; Ammonia; Asparaginase; Asparagine; Aspartic Acid; Biocatalysis; Cloning, Molecular; Enzyme Stability; Escherichia coli; Guanidine; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Protein Denaturation; Protein Unfolding; Temperature; Thermococcus; Urea | 2013 |
Carbohydrate binding and unfolding of Spatholobus parviflorus lectin: fluorescence and circular dichroism spectroscopic study.
Topics: Aspergillus niger; Carbohydrate Metabolism; Circular Dichroism; Fabaceae; Fusarium; Guanidine; Hemagglutination; Hydrogen-Ion Concentration; Ligands; Plant Lectins; Protein Denaturation; Sequence Analysis; Spectrometry, Fluorescence; Temperature; Urea | 2013 |
Automation of dimethylation after guanidination labeling chemistry and its compatibility with common buffers and surfactants for mass spectrometry-based shotgun quantitative proteome analysis.
Topics: Automation; Buffers; Equipment Design; Escherichia coli Proteins; Guanidine; Isotope Labeling; Mass Spectrometry; Methylation; Proteomics; Sodium Dodecyl Sulfate; Surface-Active Agents; Urea | 2013 |
Dodine as a protein denaturant: the best of two worlds?
Topics: Circular Dichroism; Guanidine; Guanidines; Protein Denaturation; Protein Structure, Secondary; Proteins; Recombinant Proteins; Repressor Proteins; Sodium Dodecyl Sulfate; Spectroscopy, Fourier Transform Infrared; Temperature; Urea; Viral Regulatory and Accessory Proteins | 2013 |
Spectroscopic studies on unfolding processes of apo-neuroglobin induced by guanidine hydrochloride and urea.
Topics: Anilino Naphthalenesulfonates; Apoproteins; Circular Dichroism; Globins; Guanidine; Nerve Tissue Proteins; Neuroglobin; Protein Binding; Protein Unfolding; Spectrometry, Fluorescence; Thermodynamics; Urea | 2013 |
Synthesis of saxitoxin derivatives bearing guanidine and urea groups at C13 and evaluation of their inhibitory activity on voltage-gated sodium channels.
Topics: Animals; Cell Line, Tumor; Cell Survival; Dose-Response Relationship, Drug; Guanidine; Mice; Molecular Structure; Saxitoxin; Structure-Activity Relationship; Urea; Voltage-Gated Sodium Channels | 2013 |
Thermally stable harpin, HrpZPss is sensitive to chemical denaturants: probing tryptophan environment, chemical and thermal unfolding by fluorescence spectroscopy.
Topics: Acrylamide; Bacterial Outer Membrane Proteins; Calorimetry, Differential Scanning; Guanidine; Kinetics; Protein Denaturation; Protein Stability; Protein Unfolding; Spectrometry, Fluorescence; Tryptophan; Urea | 2013 |
Dimeric argininamide-type neuropeptide Y receptor antagonists: chiral discrimination between Y1 and Y4 receptors.
Topics: Arginine; Dimerization; Fura-2; Guanidine; Humans; Models, Molecular; Protein Binding; Receptors, Neuropeptide Y; Stereoisomerism; Structure-Activity Relationship; Urea | 2013 |
Single-molecule spectroscopy of the unexpected collapse of an unfolded protein at low pH.
Topics: Cold Shock Proteins and Peptides; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Protein Unfolding; Spectrometry, Fluorescence; Urea | 2013 |
Ornithine carbamoyltransferase unfolding states in the presence of urea and guanidine hydrochloride.
Topics: Animals; Circular Dichroism; Electrophoresis, Agar Gel; Enzyme Activation; Fluorescence; Guanidine; Kinetics; Ornithine Carbamoyltransferase; Protein Folding; Protein Structure, Secondary; Sharks; Spectrophotometry, Ultraviolet; Thermodynamics; Urea | 2014 |
Structure-reactivity relationships of zwitterionic 1,3-diaza-Claisen rearrangements.
Topics: Guanidine; Molecular Structure; Thiourea; Urea | 2013 |
Asymmetric α-hydroxylation of tetralone-derived β-ketoesters by using a guanidine-urea bifunctional organocatalyst in the presence of cumene hydroperoxide.
Topics: Benzene Derivatives; Catalysis; Esters; Guanidine; Hydroxylation; Oxidation-Reduction; Stereoisomerism; Tetralones; Urea | 2013 |
Synthesis of c-di-GMP analogs with thiourea, urea, carbodiimide, and guanidinium linkages.
Topics: Carbodiimides; Cyclic GMP; Guanidine; Nucleic Acid Conformation; Urea | 2014 |
Co-aggregation of laccase and nature egg white: a simple method to prepare stable and recyclable biocatalyst.
Topics: Animals; Biocatalysis; Chickens; Cross-Linking Reagents; Egg White; Environmental Pollutants; Enzyme Stability; Enzymes, Immobilized; Fungal Proteins; Fungi; Glutaral; Guanidine; Laccase; Phenols; Protein Denaturation; Temperature; Urea | 2014 |
Effect of chaotropes on lipase back extraction recovery in the process of reverse micellar extraction.
Topics: Animals; Cetrimonium; Cetrimonium Compounds; Guanidine; Hydrogen-Ion Concentration; Lipase; Liquid Phase Microextraction; Micelles; Pancreas; Solutions; Static Electricity; Surface-Active Agents; Swine; Urea; Water | 2014 |
The effect of internal control sequence and length on the response to PCR inhibition in real-time PCR quantitation.
Topics: Bile Acids and Salts; Collagen; Cytosine; DNA Fingerprinting; Guanidine; Guanine; Humans; Humic Substances; Melanins; Microsatellite Repeats; Real-Time Polymerase Chain Reaction; Sequence Analysis, DNA; Tannins; Urea | 2014 |
Folding studies of arginine kinase from Euphausia superba using denaturants.
Topics: Animals; Arginine Kinase; Enzyme Activation; Euphausiacea; Guanidine; Kinetics; Muscles; Protein Denaturation; Protein Structure, Tertiary; Urea | 2014 |
Distribution, transition and thermodynamic stability of protein conformations in the denaturant-induced unfolding of proteins.
Topics: Animals; Carbonic Anhydrases; Cattle; Chickens; Cytochromes c; Guanidine; Muramidase; Myocardium; Protein Conformation; Protein Denaturation; Protein Unfolding; Regression Analysis; Thermodynamics; Urea | 2014 |
The Yariv reagent: behaviour in different solvents and interaction with a gum arabic arabinogalactan-protein.
Topics: Diffusion; Dimethyl Sulfoxide; Dimethylformamide; Glucosides; Guanidine; Gum Arabic; Mucoproteins; Nuclear Magnetic Resonance, Biomolecular; Phloroglucinol; Plant Proteins; Scattering, Radiation; Sodium Chloride; Solvents; Urea; Water | 2014 |
Structural functional and folding scenario of an anti platelet and thrombolytic enzyme crinumin.
Topics: Circular Dichroism; Fibrinolytic Agents; Guanidine; Hydrogen-Ion Concentration; Plant Proteins; Platelet Aggregation Inhibitors; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Temperature; Urea | 2014 |
Synthesis and biological evaluation of novel urea- and guanidine-based derivatives for the treatment of obesity-related hepatic steatosis.
Topics: 3T3-L1 Cells; Animals; Diet, High-Fat; Fatty Liver; Gene Expression Regulation; Guanidine; Humans; Leptin; Lipid Metabolism; Mice; Obesity; Urea | 2014 |
Free energy landscape of lysozyme: multiple near-native conformational states and rollover in the urea dependence of folding energy.
Topics: Guanidine; Hydrodynamics; Hydrogen-Ion Concentration; Kinetics; Muramidase; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Thermodynamics; Urea | 2014 |
Chemical- and thermal-induced unfolding of Leishmania donovani ribose-5-phosphate isomerase B: a single-tryptophan protein.
Topics: Aldose-Ketose Isomerases; Enzyme Stability; Guanidine; Leishmania donovani; Protein Unfolding; Recombinant Proteins; Temperature; Tryptophan; Urea | 2014 |
A comparative study on the aggregating effects of guanidine thiocyanate, guanidine hydrochloride and urea on lysozyme aggregation.
Topics: Circular Dichroism; Guanidine; Guanidines; Microscopy, Atomic Force; Muramidase; Protein Binding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Thiocyanates; Urea | 2014 |
A folding study of Antarctic krill (Euphausia superba) alkaline phosphatase using denaturants.
Topics: Alkaline Phosphatase; Animals; Enzyme Activation; Euphausiacea; Guanidine; Protein Denaturation; Protein Folding; Sodium Dodecyl Sulfate; Thermodynamics; Urea | 2014 |
Structural stability and unfolding properties of cutinases from Thermobifida fusca.
Topics: Actinobacteria; Carboxylic Ester Hydrolases; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Protein Conformation; Protein Unfolding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Urea | 2014 |
Stability and activity of porcine lipase against temperature and chemical denaturants.
Topics: Animals; Enzyme Stability; Guanidine; Hot Temperature; Lipase; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Unfolding; Swine; Urea | 2014 |
Inactivation and unfolding of protein tyrosine phosphatase from Thermus thermophilus HB27 during urea and guanidine hydrochloride denaturation.
Topics: Amino Acid Sequence; Enzyme Activation; Guanidine; Models, Molecular; Molecular Sequence Data; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Tyrosine Phosphatases; Sequence Alignment; Thermus thermophilus; Urea | 2014 |
Structural characteristic of the initial unfolded state on refolding determines catalytic efficiency of the folded protein in presence of osmolytes.
Topics: Biocatalysis; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Kinetics; Osmolar Concentration; Protein Denaturation; Protein Refolding; Ribonuclease, Pancreatic; Temperature; Thermodynamics; Urea | 2014 |
Guanidine hydrochloride and urea effects upon thermal stability of Glossoscolex paulistus hemoglobin (HbGp).
Topics: Animals; Annelida; Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Hemoglobins; Models, Molecular; Protein Conformation; Protein Multimerization; Protein Refolding; Protein Stability; Protein Unfolding; Thermodynamics; Urea | 2015 |
Protein denaturants at aqueous-hydrophobic interfaces: self-consistent correlation between induced interfacial fluctuations and denaturant stability at the interface.
Topics: Fungal Proteins; Guanidine; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Protein Denaturation; Protein Stability; Surface Properties; Urea; Water | 2015 |
Origin of stereocontrol in guanidine-bisurea bifunctional organocatalyst that promotes α-hydroxylation of tetralone-derived β-ketoesters: asymmetric synthesis of β- and γ-substituted tetralone derivatives via organocatalytic oxidative kinetic resolution.
Topics: Catalysis; Esters; Guanidine; Hydroxylation; Kinetics; Lignans; Models, Molecular; Molecular Conformation; Oxidation-Reduction; Stereoisomerism; Tetralones; Urea | 2015 |
Probing conformational stability and dynamics of erythroid and nonerythroid spectrin: effects of urea and guanidine hydrochloride.
Topics: Animals; Erythroid Cells; Guanidine; Protein Denaturation; Protein Multimerization; Protein Stability; Protein Structure, Quaternary; Protein Structure, Secondary; Spectrin; Urea | 2015 |
Quantitative assessments of the distinct contributions of polypeptide backbone amides versus side chain groups to chain expansion via chemical denaturation.
Topics: Amides; Amino Acid Sequence; Dose-Response Relationship, Drug; Guanidine; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Structure, Secondary; Urea; Water | 2015 |
Chemical and thermal unfolding of a global staphylococcal virulence regulator with a flexible C-terminal end.
Topics: Bacterial Proteins; Guanidine; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Multigene Family; Protein Conformation; Protein Structure, Tertiary; Protein Unfolding; Recombinant Proteins; Staphylococcus aureus; Temperature; Urea | 2015 |
Dynamic surface properties of lysozyme solutions. Impact of urea and guanidine hydrochloride.
Topics: Animals; Cattle; Elasticity; Guanidine; Kinetics; Muramidase; Protein Denaturation; Protein Structure, Tertiary; Surface Properties; Surface Tension; Urea | 2015 |
Denaturation properties and folding transition states of leghemoglobin and other heme proteins.
Topics: Arachis; Cytochromes c; Guanidine; Leghemoglobin; Myoglobin; Protein Denaturation; Protein Folding; Spectrum Analysis; Urea | 2015 |
Cooperative Unfolding of Residual Structure in Heat Denatured Proteins by Urea and Guanidinium Chloride.
Topics: Animals; Cattle; Chickens; Chymotrypsinogen; Circular Dichroism; Guanidine; Hot Temperature; Lactalbumin; Muramidase; Protein Denaturation; Protein Folding; Protein Stability; Protein Structure, Secondary; Protein Unfolding; Ribonuclease, Pancreatic; Solutions; Thermodynamics; Urea | 2015 |
Effect of guanidine hydrochloride and urea on the interaction of 6-thioguanine with human serum albumin: a spectroscopic and molecular dynamics based study.
Topics: Guanidine; Humans; Molecular Conformation; Molecular Docking Simulation; Molecular Dynamics Simulation; Protein Binding; Protein Denaturation; Protein Folding; Serum Albumin; Spectrum Analysis; Structure-Activity Relationship; Thioguanine; Urea | 2016 |
Destabilization of the Quaternary Structure of Bovine Heart Cytochrome c Oxidase upon Removal of Tightly Bound Cardiolipin.
Topics: Animals; Cardiolipins; Cattle; Electron Transport Complex IV; Guanidine; Models, Molecular; Myocardium; Protein Binding; Protein Denaturation; Protein Structure, Quaternary; Protein Subunits; Thermodynamics; Urea | 2015 |
Analysis of the pH-dependent stability and millisecond folding kinetics of horse cytochrome c.
Topics: Amino Acid Sequence; Animals; Cytochromes c; Guanidine; Horses; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Molecular Sequence Data; Myocardium; Protein Denaturation; Protein Folding; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Unfolding; Static Electricity; Thermodynamics; Urea | 2015 |
Comparison of Different Sample Preparation Protocols Reveals Lysis Buffer-Specific Extraction Biases in Gram-Negative Bacteria and Human Cells.
Topics: Artifacts; Deoxycholic Acid; Deoxyribonucleases; Gene Expression; Guanidine; HEK293 Cells; Histones; Humans; Liquid-Liquid Extraction; Proteome; Proteomics; Pseudomonas aeruginosa; Ribosomal Proteins; Species Specificity; Specimen Handling; Urea | 2015 |
Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms.
Topics: Amyloid; Animals; Biofilms; Cell Line; Creutzfeldt-Jakob Syndrome; Decontamination; Detergents; Guanidine; Mice; Neurons; Prions; Protein Denaturation; Scrapie; Sodium Dodecyl Sulfate; Surgical Instruments; Thiourea; Urea | 2015 |
Effect of Osmolytes and Guanidinium Chloride on the Enzymatic Properties of Bovine α-Chymotrypsin.
Topics: Animals; Betaine; Biocatalysis; Catalytic Domain; Cattle; Chymotrypsin; Guanidine; Kinetics; Methylamines; Peptides; Urea | 2016 |
Reorientation Motion and Preferential Interactions of a Peptide in Denaturants and Osmolyte.
Topics: Fluorescence Polarization; Guanidine; Molecular Dynamics Simulation; Motion; Osmolar Concentration; Peptides; Proline; Protein Denaturation; Urea; Water | 2016 |
Use of Protein Folding Reagents.
Topics: Biochemistry; Guanidine; Indicators and Reagents; Kinetics; Oxidation-Reduction; Protein Denaturation; Protein Folding; Protein Stability; Proteins; Reducing Agents; Solvents; Sulfhydryl Reagents; Urea | 2016 |
Denaturation induced aggregation in α-crystallin: differential action of chaotropes.
Topics: alpha-Crystallins; Amyloid; Circular Dichroism; Guanidine; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Urea | 2016 |
Probing the molecular forces involved in binding of selected volatile flavour compounds to salt-extracted pea proteins.
Topics: Aldehydes; Benzaldehydes; Disulfides; Gas Chromatography-Mass Spectrometry; Guanidine; Hydrogen Bonding; Ketones; Pisum sativum; Plant Proteins; Propylene Glycol; Reducing Agents; Sodium Chloride, Dietary; Taste; Urea; Volatile Organic Compounds | 2016 |
Probing Selection Mechanism of the Most Favorable Conformation of a Dipeptide in Chaotropic and Kosmotropic Solution.
Topics: Dipeptides; Fluorescence Polarization; Guanidine; Hydrogen-Ion Concentration; Molecular Dynamics Simulation; Temperature; Thermodynamics; Urea | 2016 |
Conformational Stability of the NH2-Terminal Propeptide of the Precursor of Pulmonary Surfactant Protein SP-B.
Topics: Amino Acid Sequence; Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Humans; Hydrodynamics; Kinetics; Osmolar Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Protein Precursors; Protein Stability; Protein Structure, Secondary; Protein Unfolding; Proteolipids; Pulmonary Surfactant-Associated Protein B; Sodium Chloride; Spectrometry, Fluorescence; Thermodynamics; Urea | 2016 |
Refolding of Aggregation-Prone ScFv Antibody Fragments Assisted by Hydrophobically Modified Poly(sodium acrylate) Derivatives.
Topics: Acrylic Resins; Circular Dichroism; Dialysis; Fluorescence; Guanidine; Hydrodynamics; Hydrophobic and Hydrophilic Interactions; Protein Aggregates; Protein Folding; Protein Structure, Secondary; Single-Chain Antibodies; Urea | 2017 |
Different States of Acrylodan-Labeled 3'5'-Cyclic Adenosine Monophosphate Dependent Protein Kinase Catalytic Subunits in Denaturant Solutions.
Topics: 2-Naphthylamine; Buffers; Catalytic Domain; Cyclic AMP-Dependent Protein Kinases; Fluorescent Dyes; Guanidine; Humans; Hydrophobic and Hydrophilic Interactions; Morpholines; Protein Denaturation; Protein Unfolding; Spectrometry, Fluorescence; Staining and Labeling; Urea | 2016 |
Investigating the folding pathway and substrate induced conformational changes in B. malayi Guanylate kinase.
Topics: Adenosine Triphosphate; Animals; Brugia malayi; Cross-Linking Reagents; Escherichia coli; Gene Expression; Glutaral; Guanidine; Guanosine Monophosphate; Guanylate Kinases; Helminth Proteins; Molecular Docking Simulation; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Urea | 2017 |
Identification of protein secondary structures by laser induced autofluorescence: A study of urea and GnHCl induced protein denaturation.
Topics: Animals; Cattle; Fluorescence; Guanidine; Humans; Lasers; Protein Denaturation; Protein Structure, Secondary; Proteins; Spectrometry, Fluorescence; Urea | 2017 |
Probing Small Molecule Binding to Unfolded Polyprotein Based on its Elasticity and Refolding.
Topics: Elasticity; Guanidine; Models, Molecular; Protein Denaturation; Protein Refolding; Proteins; Sodium Dodecyl Sulfate; Urea | 2016 |
In vitro activity and stability of pure human salivary aldehyde dehydrogenase.
Topics: Aldehyde Dehydrogenase; Enzyme Stability; Glycerol; Guanidine; Humans; Protein Denaturation; Saliva; Sodium Chloride; Temperature; Urea | 2017 |
Synthesis and Biological Evaluation of Amidinourea and Triazine Congeners as Inhibitors of MDA-MB-231 Human Breast Cancer Cell Proliferation.
Topics: Antineoplastic Agents; Cell Line, Tumor; Cell Proliferation; Drug Design; Guanidine; Guanidines; Humans; Morpholines; Structure-Activity Relationship; Triazines; Urea | 2017 |
Comparative stability of Major Facilitator Superfamily transport proteins.
Topics: Bacterial Proteins; Guanidine; Ligands; Membrane Transport Proteins; Models, Molecular; Protein Domains; Protein Stability; Protein Structure, Secondary; Protein Unfolding; Urea | 2017 |
Effect of mammalian kidney osmolytes on the folding pathway of sheep serum albumin.
Topics: Animals; Betaine; Guanidine; Inositol; Kidney; Osmosis; Protein Denaturation; Protein Folding; Protein Stability; Serum Albumin; Sheep; Sorbitol; Thermodynamics; Urea | 2017 |
Thermodynamic and structural effect of urea and guanidine chloride on the helical and on a hairpin fragment of GB1 from molecular simulations.
Topics: Bacterial Proteins; Binding Sites; Guanidine; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Denaturation; Protein Folding; Protein Interaction Domains and Motifs; Streptococcus; Thermodynamics; Urea | 2017 |
Structural analyses combined with small-angle X-ray scattering reveals that the retention of heme is critical for maintaining the structure of horseradish peroxidase under denaturing conditions.
Topics: Circular Dichroism; Guanidine; Heme; Horseradish Peroxidase; Models, Molecular; Protein Denaturation; Protein Structure, Tertiary; Protein Unfolding; Temperature; Urea; X-Ray Diffraction | 2017 |
Stability of different influenza subtypes: How can high hydrostatic pressure be a useful tool for vaccine development?
Topics: Animals; Guanidine; Humans; Hydrostatic Pressure; Influenza A virus; Influenza A Virus, H1N1 Subtype; Influenza A Virus, H3N2 Subtype; Influenza A Virus, H3N8 Subtype; Temperature; Urea; Vaccines; Virus Inactivation | 2017 |
Fluorescence and circular dichroism studies on the accessibility of tryptophan residues and unfolding of a jacalin-related α-d-galactose-specific lectin from mulberry (Morus indica).
Topics: Cesium; Circular Dichroism; Galactose; Guanidine; Guanidines; Hydrogen-Ion Concentration; Iodides; Lectins; Morus; Plant Lectins; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Unfolding; Spectrometry, Fluorescence; Temperature; Thiocyanates; Tryptophan; Urea | 2017 |
Effect of chemical denaturants on the conformational stability of GyrB subunit of DNA gyrase from Salmonella enterica serovar Typhi.
Topics: DNA Gyrase; Dose-Response Relationship, Drug; Enzyme Stability; Guanidine; Protein Denaturation; Protein Domains; Protein Subunits; Salmonella typhi; Urea | 2017 |
Comparison between chaotropic and detergent-based sample preparation workflow in tendon for mass spectrometry analysis.
Topics: Animals; Detergents; Guanidine; Horses; Proteins; Proteomics; Tandem Mass Spectrometry; Tendons; Urea; Workflow | 2017 |
Structure and Functional Characterisation of a Distinctive β-Lactamase from an Environmental Strain EMB20 of Bacillus cereus.
Topics: Bacillus cereus; beta-Lactamase Inhibitors; beta-Lactamases; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Guanidine; Protein Structure, Secondary; Spectrometry, Fluorescence; Substrate Specificity; Ultrafiltration; Urea | 2018 |
Quantifying Protection in Disordered Proteins Using Millisecond Hydrogen Exchange-Mass Spectrometry and Peptic Reference Peptides.
Topics: Algorithms; Animals; Chromatography, High Pressure Liquid; CREB-Binding Protein; Deuterium Exchange Measurement; Guanidine; Humans; Indicators and Reagents; Intrinsically Disordered Proteins; Kinetics; Mice; Models, Molecular; Nuclear Receptor Coactivator 3; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Interaction Domains and Motifs; Recombinant Proteins; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Urea | 2017 |
Effect of replacing main-chain ureas with thiourea and guanidinium surrogates on the bactericidal activity of membrane active oligourea foldamers.
Topics: Animals; Anti-Bacterial Agents; Bacillus anthracis; Cell Survival; Dose-Response Relationship, Drug; Guanidine; Mice; Microbial Sensitivity Tests; Molecular Structure; RAW 264.7 Cells; Structure-Activity Relationship; Thiourea; Urea | 2017 |
Insight into the functional and structural transition of garlic phytocystatin induced by urea and guanidine hydrochloride: A comparative biophysical study.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Cystatins; Fluorescent Dyes; Garlic; Guanidine; Protein Denaturation; Protein Folding; Protein Stability; Protein Structure, Secondary; Spectrometry, Fluorescence; Urea | 2018 |
Structural plasticity of T4 transcription co-activator gp33 revealed by a protease-resistant unfolded state.
Topics: Guanidine; Models, Molecular; Peptide Hydrolases; Protein Conformation; Protein Unfolding; Urea; Viral Proteins | 2017 |
Effect of chaotropes on the kinetics of iron release from ferritin by flavin nucleotides.
Topics: Animals; Electron Transport; Ferritins; Flavin Mononucleotide; Guanidine; Horses; Humans; Hydroquinones; Iron; Kinetics; Octoxynol; Oxidation-Reduction; Urea | 2017 |
Isothermal chemical denaturation of large proteins: Path-dependence and irreversibility.
Topics: Animals; Antibodies, Monoclonal; Cattle; Guanidine; Protein Denaturation; Protein Refolding; Proteins; Serum Albumin, Bovine; Spectrometry, Fluorescence; Thermodynamics; Urea | 2017 |
Cation-Induced Stabilization and Denaturation of DNA Origami Nanostructures in Urea and Guanidinium Chloride.
Topics: Cations; DNA; Guanidine; Microscopy, Atomic Force; Nucleic Acid Conformation; Nucleic Acid Denaturation; Transition Temperature; Urea | 2017 |
Counteraction of the deleterious effects of urea on structure and stability of mammalian kidney proteins by osmolytes.
Topics: Animals; Betaine; Guanidine; Inositol; Kidney; Mammals; Osmolar Concentration; Protein Stability; Proteins; Sheep; Sorbitol; Urea | 2018 |
Denaturant-specific effects on the structural energetics of a protein-denatured ensemble.
Topics: Bacterial Proteins; Fluorescence Resonance Energy Transfer; Guanidine; Protein Denaturation; Protein Domains; Thermodynamics; Urea | 2018 |
Changing relations between proteins and osmolytes: a choice of nature.
Topics: Amino Acids; Guanidine; Hydrophobic and Hydrophilic Interactions; Methylamines; Molecular Dynamics Simulation; Polymers; Protein Denaturation; Protein Folding; Protein Stability; Proteins; Synucleins; Thermodynamics; Urea | 2018 |
Investigation of the thermal shift assay and its power to predict protein and virus stabilizing conditions.
Topics: Albumins; Cells, Cultured; Drug Compounding; Drug Stability; Guanidine; Hydrogen-Ion Concentration; Immunoglobulin G; Measles virus; Molecular Dynamics Simulation; Mumps virus; Ovalbumin; Polymerization; Potassium Cyanide; Protein Denaturation; Protein Stability; Transition Temperature; Urea; Viral Envelope Proteins | 2018 |
Biochemical Properties and Catalytic Specificity of a Novel Neutral Serine Peptidase Secreted by Fungus Pyrenochaetopsis sp.
Topics: Ascomycota; Biocatalysis; Enzyme Inhibitors; Guanidine; Metals; Serine Proteases; Serine Proteinase Inhibitors; Substrate Specificity; Surface-Active Agents; Temperature; Urea | 2019 |
Protective Effect of Biological Osmolytes against Heat- and Chaotropic Agent-Induced Denaturation of
Topics: Bacillus licheniformis; Escherichia coli; gamma-Glutamyltransferase; Glycerol; Guanidine; Hot Temperature; Methylamines; Osmolar Concentration; Protein Denaturation; Protein Stability; Protein Structure, Secondary; Recombinant Proteins; Sorbitol; Spectrometry, Fluorescence; Sucrose; Urea | 2018 |
Single-pot, solid-phase-enhanced sample preparation for proteomics experiments.
Topics: Buffers; Chemical Fractionation; Detergents; Guanidine; HEK293 Cells; Humans; Hydrophobic and Hydrophilic Interactions; Isothiocyanates; Proteome; Proteomics; Solid Phase Microextraction; Solvents; Specimen Handling; Urea | 2019 |
Single-Molecule Förster Resonance Energy Transfer Measurement Reveals the Dynamic Partially Ordered Structure of the Epidermal Growth Factor Receptor C-Tail Domain.
Topics: Amino Acid Sequence; Circular Dichroism; ErbB Receptors; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Guanidine; Humans; Intrinsically Disordered Proteins; Mutation; Phosphorylation; Potassium Chloride; Protein Conformation, beta-Strand; Protein Domains; Urea | 2019 |
Synthesis, biological evaluation and mode of action studies of novel amidinourea inhibitors of hepatitis C virus (HCV).
Topics: Antiviral Agents; Cell Line; Guanidine; Hepacivirus; Humans; Urea; Virus Replication | 2019 |
Opposing reactions in coenzyme A metabolism sensitize
Topics: Acyl Carrier Protein; Animals; Bacterial Proteins; Catalytic Domain; Coenzyme A; Drug Resistance, Bacterial; Female; Guanidine; Hydrolases; Lipid Metabolism; Loss of Function Mutation; Mice; Mice, Inbred BALB C; Mycobacterium tuberculosis; Operon; Protein Binding; Protein Structure, Tertiary; Small Molecule Libraries; Transferases (Other Substituted Phosphate Groups); Urea | 2019 |
Networks of electrostatic and hydrophobic interactions modulate the complex folding free energy surface of a designed βα protein.
Topics: Guanidine; Hydrophobic and Hydrophilic Interactions; Nuclear Magnetic Resonance, Biomolecular; Protein Folding; Protein Structure, Secondary; Static Electricity; Urea | 2019 |
Relative Solvent Exposure of the Alpha-Helix and Beta-Sheet in Water Determines the Initial Stages of Urea and Guanidinium Chloride-Induced Denaturation of Alpha/Beta Proteins.
Topics: Guanidine; Humans; Molecular Dynamics Simulation; Protein Conformation; Protein Denaturation; Proteins; Solvents; Thermodynamics; Urea | 2019 |
Distinct and Nonadditive Effects of Urea and Guanidinium Chloride on Peptide Solvation.
Topics: Amino Acid Sequence; Guanidine; Hydrogen Bonding; Peptides; Protein Conformation, alpha-Helical; Protein Denaturation; Urea | 2019 |
Alteration of the groove width of DNA induced by the multimodal hydrogen bonding of denaturants with DNA bases in its grooves affects their stability.
Topics: Base Pairing; DNA; Guanidine; Hydrogen Bonding; Molecular Dynamics Simulation; Nucleic Acid Conformation; Urea; Water | 2020 |
In vitro characterization, ADME analysis, and histological and toxicological evaluation of BM1, a macrocyclic amidinourea active against azole-resistant Candida strains.
Topics: Animals; Antifungal Agents; Azoles; Candida; Candidiasis; Drug Resistance, Fungal; Guanidine; Microbial Sensitivity Tests; Rats; Urea | 2020 |
Effects of natural mutations (L94I and L94V) on the stability and mechanism of folding of horse cytochrome c: A combined in vitro and molecular dynamics simulations approach.
Topics: Amino Acid Substitution; Animals; Cytochromes c; Guanidine; Horses; Molecular Dynamics Simulation; Mutation; Protein Denaturation; Protein Folding; Spectrum Analysis; Thermodynamics; Urea | 2020 |
Global analysis of protein stability by temperature and chemical denaturation.
Topics: Carrier Proteins; Guanidine; Kinetics; Peptides; Plant Proteins; Protein Conformation; Protein Denaturation; Protein Stability; Thermodynamics; Urea | 2020 |
Solving the Conundrum: Widespread Proteins Annotated for Urea Metabolism in Bacteria Are Carboxyguanidine Deiminases Mediating Nitrogen Assimilation from Guanidine.
Topics: Allophanate Hydrolase; Ammonia; Carbon-Nitrogen Ligases; Catalysis; Citrullination; Guanidine; Hydrolases; Metabolic Networks and Pathways; Molecular Sequence Annotation; Nitrogen; Protein Subunits; Pseudomonas syringae; Urea | 2020 |
Attachment of Proteins to a Hydroxyl-Terminated Surface Eliminates the Stabilizing Effects of Polyols.
Topics: Guanidine; Hydroxyl Radical; Polymers; Protein Folding; Proteins; Sulfates; Surface Properties; Thermodynamics; Urea | 2020 |
Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses.
Topics: Analytic Sample Preparation Methods; Animals; Cells, Cultured; Exosomes; Guanidine; Humans; Immunoprecipitation; Mass Spectrometry; Proteins; Proteomics; Urea | 2021 |
Filling in the Gaps in Metformin Biodegradation: a New Enzyme and a Metabolic Pathway for Guanylurea.
Topics: Ammonia; Bacterial Proteins; Biodegradation, Environmental; Biomineralization; Genome, Bacterial; Guanidine; Hydrolases; Metabolic Networks and Pathways; Metformin; Multigene Family; Pseudomonas mendocina; Substrate Specificity; Urea; Wastewater; Water Pollutants, Chemical | 2021 |
Crowding Milleu stabilizes apo-myoglobin against chemical-induced denaturation: Dominance of hardcore repulsions in the heme devoid protein.
Topics: Animals; Apoproteins; Dextrans; Ficoll; Guanidine; Heme; Horses; Macromolecular Substances; Myoglobin; Protein Conformation; Protein Denaturation; Protein Stability; Spectrophotometry, Ultraviolet; Thermodynamics; Urea | 2021 |
Structurally distinct unfolding intermediates formed from a staphylococcal capsule-producing enzyme retained NADPH binding activity.
Topics: Circular Dichroism; Guanidine; Kinetics; NADP; Protein Denaturation; Protein Folding; Staphylococcus aureus; Urea | 2022 |
Synthesis and antiproliferative screening of novel doubly modified colchicines containing urea, thiourea and guanidine moieties.
Topics: Antineoplastic Agents; Cell Line, Tumor; Cell Proliferation; Colchicine; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Guanidine; Humans; Molecular Structure; Structure-Activity Relationship; Thiourea; Urea | 2021 |
Characterization of the structural forces governing the reversibility of the thermal unfolding of the human acidic fibroblast growth factor.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cell Proliferation; Fibroblast Growth Factor 1; Guanidine; Heparin; Humans; Mice; Mutant Proteins; Mutation; NIH 3T3 Cells; Protein Conformation; Protein Denaturation; Protein Stability; Protein Unfolding; Static Electricity; Temperature; Urea | 2021 |
Oxidative stress as a potential mechanism by which guanylurea disrupts the embryogenesis of Danio rerio.
Topics: Animals; Diabetes Mellitus, Type 2; Embryo, Nonmammalian; Embryonic Development; Guanidine; Oxidative Stress; Urea; Water Pollutants, Chemical; Zebrafish | 2021 |
Synthesis and Biological Evaluation of Amidinourea Derivatives against Herpes Simplex Viruses.
Topics: Acyclovir; Antiviral Agents; Drug Resistance, Viral; Guanidine; Herpes Simplex; Herpesvirus 1, Human; Humans; Simplexvirus; Urea | 2021 |
Probing the Internal Dynamics and Shape of Simple Peptides in Urea, Guanidinium Hydrochloride, and Proline Solutions with Time-Resolved Fluorescence Anisotropy and Atomistic Cosolvent Simulations.
Topics: Fluorescence Polarization; Guanidine; Peptides; Proline; Urea | 2021 |
Chronic exposure to environmentally relevant concentrations of guanylurea induces neurotoxicity of Danio rerio adults.
Topics: Acetylcholinesterase; Animals; Guanidine; Oxidative Stress; Urea; Water Pollutants, Chemical; Zebrafish | 2022 |
In Vitro and In Vivo Inhibition of the
Topics: Bacterial Proteins; Binding Sites; Crystallography, X-Ray; Guanidine; Kinetics; Microbial Sensitivity Tests; Molecular Conformation; Molecular Dynamics Simulation; Mycobacterium tuberculosis; Structure-Activity Relationship; Transferases (Other Substituted Phosphate Groups); Urea | 2022 |
On the origin of chaotrope-modulated electrocatalytic activity of cytochrome
Topics: Cytochromes c; Guanidine; Reactive Oxygen Species; Urea; Water | 2022 |
Urea induced unfolding of rai seed cystatin: Influence of glycerol as a chemical chaperone.
Topics: Circular Dichroism; Cystatins; Glycerol; Guanidine; Peptide Hydrolases; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Urea | 2023 |
Topics: Ferritins; Graphite; Guanidine; Protein Denaturation; Urea | 2023 |