guanidine has been researched along with cysteine in 117 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 6 (5.13) | 18.7374 |
| 1990's | 40 (34.19) | 18.2507 |
| 2000's | 56 (47.86) | 29.6817 |
| 2010's | 12 (10.26) | 24.3611 |
| 2020's | 3 (2.56) | 2.80 |
| Authors | Studies |
|---|---|
| Chen, M; Hu, C; Suzuki, A; Thakkar, S; Tong, W; Yu, K | 1 |
| Brand, L; James, E; Stites, W; Wu, PG | 1 |
| Le, HV; Syto, R; Trotta, PP; Windsor, WT | 1 |
| Becker, GW; Brems, DN; Brown, PL | 1 |
| Ballery, N; Betton, JM; Desmadril, M; Hall, L; Minard, P; Mouawad, L; Perahia, D; Yon, JM | 1 |
| Ebina, S; Lerner, RA; Wright, PE | 1 |
| Beharry, S; Bragg, PD | 1 |
| Howell, EE; Kraut, J; Oatley, SJ; Villafranca, JE; Xuong, NH | 1 |
| Snyder, GH | 1 |
| Arakawa, T; Boone, TC; Kenney, WC; Narhi, LO; Prestrelski, SJ | 1 |
| Gonnelli, M; Strambini, GB | 1 |
| Hellekant, G; Markley, JL; Ming, D | 1 |
| Dirr, H; Sluis-Cremer, N | 1 |
| Tsujikawa, T; Uyeda, K; Watanabe, F | 1 |
| Andrieux, M; Desmadril, M; Guittet, E; Leroy, E; Minard, P; Mouratou, B; Ritco-Vonsovici, M; Yon, JM | 1 |
| Ocheretina, O; Scheibe, R | 1 |
| Goldfarb, D; Kreimer, DI; Silman, I; Szosenfogel, R; Weiner, L | 1 |
| Landt, O; Mayr, LM; Schmid, FX; Willbold, D | 1 |
| Frieden, C; Jiang, N | 1 |
| Bächinger, HP; Davis, JM | 1 |
| Auld, DS; Betz, SF; Doyle, DF; Pielak, GJ; Saunders, AJ; Young, GB | 1 |
| Dallas, JS; Desai, RK; Kurosky, A; Prabhakar, BS; Seetharamaiah, GS | 1 |
| Blau, N; Bürgisser, DM; Heizmann, CW; Hunziker, P; Redweik, U; Thöny, B | 1 |
| Carlsson, U; Freskgård, PO; Jonsson, BH; Kihlgren, A; Mårtensson, LG; Svensson, M | 1 |
| Johnson, JL; Raushel, FM | 1 |
| Haniu, M; Hui, JO; Katta, V; Le, J; Rohde, MF; Rosenfeld, R | 1 |
| Camacho, CJ; Thirumalai, D | 1 |
| Kreimer, DI; Shin, I; Shnyrov, VL; Silman, I; Villar, E; Weiner, L | 1 |
| Beltrán, C; Darszon, A; Ferreira, ST; Garzón-Rodríguez, W; Gómez-Puyou, A; Sepúlveda-Becerra, MA; Strasser, RJ | 1 |
| Bedouelle, H; Blondel, A; Nageotte, R | 1 |
| Fetrow, JS; Qu, K; Scholes, CP; Sienkiewicz, A; Vaughn, JL | 1 |
| Burstein, Y; Peretz, M; Weiner, LM | 1 |
| Helmkamp, GM; Tremblay, JM; Voziyan, PA; Yarbrough, LR | 1 |
| Abian, J; Caminade, E; Canosa, D; Carrascal, M; Daniel, R; Le Goffic, F; Martel, A | 1 |
| Carlsson, U; Hammarström, P; Jonsson, BH; Kalman, B | 1 |
| Artigues, A; Iriarte, A; Martinez-Carrion, M; Mattingly, JR; Torella, C | 1 |
| Gehrig, P; Gervasoni, P; Plückthun, A | 1 |
| Baker, GA; Brennan, JD; Bright, FV; Doody, MA; Flora, K | 1 |
| Ekuni, A; Fujiwara, Y; Hao, W; Hayatsu, H; Munakata, N; Negishi, K; Ono, T; Yang, SJ | 1 |
| Clottes, E; Couthon, F; Ebel, C; Leydier, C; Marcillat, O; Vial, C | 1 |
| Balaram, H; Balaram, P; Gokhale, RS; Ray, SS | 1 |
| Brancaccio, A; Engel, J; Jenö, P | 1 |
| Dadlez, M; Zdanowski, K | 1 |
| Brandi, L; Caserta, E; Gualerzi, CO; Krafft, C; Misselwitz, R; Welfle, H; Welfle, K | 1 |
| Marqusee, S; Parker, MJ | 1 |
| Baase, WA; Breyer, WA; Bustamante, C; Cecconi, C; Dahlquist, FW; Haack, JA; Matthews, BW; Vetter, IR; Yang, G | 1 |
| Izutani, K; Koumoto, Y; Kubo, Y; Noda, Y; Segawa, S; Tachibana, H; Takai, M; Yokota, A | 1 |
| Aloor, JJ; Panse, VG; Surolia, A; Swaminathan, CP; Varadarajan, R | 1 |
| Grütter, MG; Hunziker, P; Lüthy, L; Mittl, PR | 1 |
| Chakraborty, S; Peng, Z | 1 |
| Deshpande, VV; Ghatge, MS; Tanksale, AM; Vernekar, JV | 1 |
| Eastman, P; Fink, AL; Nishimura, C; Riley, R | 1 |
| Blaber, M; Blaber, SI; Culajay, JF; Khurana, A | 1 |
| Ferguson, SJ; Tomlinson, EJ | 1 |
| Hirose, M; Muralidhara, BK | 1 |
| Carlsson, U; Hammarstrom, P; Persson, M | 1 |
| Kunugi, S; Mitani, D; Tanaka, N | 1 |
| Brandt, M; Chambers, PA; Daines, RA; Doyle, ML; Gentry, DR; Khandekar, SS; Konstantinidis, AK; Lonsdale, JT; Silverman, C; Van Aller, GS; Warren, P; Xiang, H | 1 |
| Alam, SL; Butler, MC; Feng, Z; Loh, SN | 1 |
| Baker, GA; Bright, FV; Hartnett, AM; Kane, MA; Maloney, TD; Pandey, S | 1 |
| Bunting, KA; Cooper, JB; Tickle, IJ; Young, DB | 1 |
| Grutter, MG; Luthy, L; Mittl, PR | 1 |
| Sridevi, K; Udgaonkar, JB | 1 |
| Caughey, B; Raymond, LD; Scheraga, HA; Welker, E | 1 |
| Luo, J; Zhang, TH; Zhou, JM | 1 |
| Bennaars-Eiden, A; Bernlohr, DA; Ferrington, DA; Hertzel, AV; Higgins, L; Kapphahn, RJ | 1 |
| Lilie, H; Rudolph, R; Winter, J | 1 |
| Czupryn, MJ; Marzilli, LA; Rouse, JC; Zhang, W | 1 |
| Berndt, KD; Chiaraluce, R; Consalvi, V; Ladenstein, R; Lebbink, JH | 1 |
| Carlsson, U; Karlsson, M; Mårtensson, LG | 1 |
| Devenish, RJ; Khan, MA; Nagley, P; Roucou, X; Stephens, AN | 1 |
| Anderluh, G; Boetzel, R; Hong, Q; Lakey, JH; MacDonald, C; Moore, GR; Virden, R | 1 |
| Bakalova, A; Kuciel, R; Mazurkiewicz, A; Wójciak, P | 1 |
| Alanen, HI; Howard, MJ; Jäntti, HP; Kellokumpu, S; Lappi, AK; Rautio, SM; Ruddock, LW; Williamson, RA | 1 |
| Chiba, H; Hayashi, R; Mima, J; Narita, Y | 1 |
| Narayan, M; Scheraga, HA; Welker, E | 1 |
| RAPOPORT, S; SARKAR, SR | 1 |
| KOMORIYAMA, K | 1 |
| Nagy, JK; Sanders, CR | 1 |
| Ermácora, MR; Ferreyra, RG; Gebhard, LG; Risso, VA; Rossi, JP; Santos, J | 1 |
| Imoto, T; Shioi, S; Ueda, T | 1 |
| Carlsson, U; Karlsson, C; Karlsson, M; Mårtensson, LG | 1 |
| Babul, J; Báez, M; Cabrera, R; Caniuguir, A; Guixé, V; Vásquez, CC | 1 |
| Favilla, R; Grolli, S; Mazzini, A; Parisi, M; Ramoni, R; Tibor Sorbi, R | 1 |
| Chang, JY; Jiang, C | 1 |
| Patteson, KG; Stadtman, TC; Trivedi, N | 1 |
| Abel, J; Barnes, JM; Brems, DN; King, J; Maloney, KM; Pipes, G; Raso, SW; Treuheit, MJ | 1 |
| Basu, G; Chakrabarti, P; Saha, RP | 1 |
| Ho, WK; Leung, MY | 1 |
| Krishnamoorthy, G; Saxena, AM; Udgaonkar, JB | 1 |
| Chang, JY; Lin, CC; Lu, BY | 1 |
| Beach, M; Colón, W; Meinhold, D; Osuna, R; Shao, Y | 1 |
| Andújar-Sánchez, M; Clemente-Jiménez, JM; Jara-Pérez, V; Las Heras-Vázquez, FJ; Martínez-Rodríguez, S; Neira, JL; Rodríguez-Vico, F | 1 |
| Baskakov, IV; Bocharova, OV; Breydo, L; Makarava, N; Sun, Y; Yang, Q | 1 |
| Buschauer, A; Dove, S; Ghorai, P; Kraus, A; Preuss, H; Seifert, R | 1 |
| Kamchonwongpaisan, S; Taweechai, S; Thebtaranonth, Y; Thongpanchang, C; Yuthavong, Y | 1 |
| Ichiki, S; Kobayashi, Y; Mita, H; Nakamura, S; Nakasone, K; Ogawa, K; Sambongi, Y; Sonoyama, T; Takayama, SJ; Takeda, T; Uchiyama, S; Yamamoto, Y | 1 |
| Meng, FG; Mu, H; Park, YD; Yang, JM; Zhou, SM | 1 |
| Chen, HM; Chow, CY; Fang, HJ; Hu, CK; Tsong, TY; Wu, MC | 1 |
| Isacoff, EY; Tombola, F; Ulbrich, MH | 1 |
| Bellini, T; Buscaglia, M; Longhi, R; Soranno, A | 1 |
| Liu, P; Meng, X; Qu, P; Wang, CC; Zhao, XS | 1 |
| de Kort, BJ; Nabuurs, SM; van Mierlo, CP; Westphal, AH | 1 |
| Chen, Y; Lapidus, LJ; Parrini, C; Taddei, N | 1 |
| Chen, R; Feng, J; Li, Y; Pang, XF; Ren, ZL; Tang, LX; Zhang, T | 1 |
| Ito, T; Kurokawa, T; Mastui, H; Shintani, Y; Tsuji, I | 1 |
| Kajihara, Y; Morooka, K; Okamoto, R | 1 |
| Garza-Ramos, G; Guzman-Luna, V | 1 |
| Bhattacharyya, K; Chattoraj, S; Chowdhury, R; Sen Mojumdar, S | 1 |
| Li, X; Mao, L; Wang, F; Yang, L; Yu, P | 1 |
| Basak, S; Bera, K; Nag, M | 1 |
| Eger, BT; Kawaguchi, Y; Matsumura, T; Nishino, T; Okamoto, K; Pai, EF | 1 |
| Hassani, L; Mosaddegh, B; Rasti, B; Sajedi, RH; Shirin Shahangian, S; Takalloo, Z | 1 |
| Ma, H; Ó'Fágáin, C; O'Kennedy, R | 1 |
| Fan, K; Hong, Y; Hu, H; Hu, Q; Raston, CL; Tang, Y; Zhang, X | 1 |
| Cai, S; Chen, G; Chu, X; Guo, DS; He, G; Li, B; Tang, H; Tian, HW; Wan, Z; Xue, XS; Zheng, H | 1 |
| Anzaki, K; Denda, M; Harada, N; Inagaki, N; Kobayashi, D; Kyan, R; Ohkawachi, K; Otaka, A; Shigenaga, A; Yasuda, T | 1 |
1 review(s) available for guanidine and cysteine
| Article | Year |
|---|---|
DILIrank: the largest reference drug list ranked by the risk for developing drug-induced liver injury in humans.
Topics: Chemical and Drug Induced Liver Injury; Databases, Factual; Drug Labeling; Humans; Pharmaceutical Preparations; Risk | 2016 |
116 other study(ies) available for guanidine and cysteine
| Article | Year |
|---|---|
Compact denatured state of a staphylococcal nuclease mutant by guanidinium as determined by resonance energy transfer.
Topics: Amino Acid Sequence; Cysteine; Energy Transfer; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Kinetics; Lysine; Mathematics; Micrococcal Nuclease; Mutagenesis, Site-Directed; Naphthalenesulfonates; Protein Denaturation; Recombinant Proteins; Tryptophan | 1992 |
Analysis of the conformation and stability of Escherichia coli derived recombinant human interleukin 4 by circular dichroism.
Topics: Circular Dichroism; Cysteine; Cystine; Escherichia coli; Guanidine; Guanidines; Hydrogen-Ion Concentration; Interleukin-4; Lymphocyte Activation; Protein Conformation; Protein Denaturation; Recombinant Proteins; T-Lymphocytes; Thermodynamics | 1991 |
Equilibrium denaturation of human growth hormone and its cysteine-modified forms.
Topics: Alkylation; Chemical Phenomena; Chemistry, Physical; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Disulfides; Escherichia coli; Growth Hormone; Guanidine; Guanidines; Humans; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Thermodynamics | 1990 |
Introduction of internal cysteines as conformational probes in yeast phosphoglycerate kinase.
Topics: Catalysis; Circular Dichroism; Cysteine; Drug Stability; Guanidine; Guanidines; Kinetics; Molecular Structure; Mutation; Phosphoglycerate Kinase; Protein Conformation; Saccharomyces cerevisiae; Sulfhydryl Compounds; Thermodynamics | 1990 |
Chemical modification of bovine pancreatic trypsin inhibitor for single site coupling of immunogenic peptides for NMR conformational analysis.
Topics: Acetylation; Aprotinin; Chemical Phenomena; Chemistry; Chymotrypsin; Cross-Linking Reagents; Cysteine; Dimyristoylphosphatidylcholine; Guanidine; Guanidines; Hydrogen-Ion Concentration; Lysine; Magnetic Resonance Spectroscopy; Methylurea Compounds; Peptides; Protein Conformation; Succinimides; Trypsin | 1989 |
Conformational change in beef-heart mitochondrial F1 ATPase to ATP synthesis mode induced by dimethylsulfoxide and ATP revealed by sulfhydryl group labeling.
Topics: Adenosine Triphosphate; Animals; Cattle; Cysteine; Dimethyl Sulfoxide; Dithionitrobenzoic Acid; Guanidine; Guanidines; Mitochondria, Heart; Protein Conformation; Proton-Translocating ATPases | 1989 |
An engineered disulfide bond in dihydrofolate reductase.
Topics: Amino Acid Sequence; Cloning, Molecular; Cysteine; Disulfides; Escherichia coli; Genes; Genes, Bacterial; Guanidine; Guanidines; Models, Molecular; Mutation; Protein Conformation; Tetrahydrofolate Dehydrogenase; X-Ray Diffraction | 1987 |
Intramolecular disulfide loop formation in a peptide containing two cysteines.
Topics: Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Glutathione; Guanidine; Guanidines; Iodoacetates; Kinetics; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Trypsin Inhibitor, Kunitz Soybean; Trypsin Inhibitors; Urea | 1987 |
Cysteine 17 of recombinant human granulocyte-colony stimulating factor is partially solvent-exposed.
Topics: Amino Acid Sequence; Animals; CHO Cells; Cloning, Molecular; Cricetinae; Cysteine; Disulfides; Escherichia coli; Glycosylation; Granulocyte Colony-Stimulating Factor; Guanidine; Guanidines; Humans; Hydrogen-Ion Concentration; Kinetics; Mutagenesis, Site-Directed; Point Mutation; Protein Conformation; Recombinant Proteins; Serine; Solvents; Spectrophotometry, Infrared; Transfection | 1993 |
Phosphorescence lifetime of tryptophan in proteins.
Topics: Amino Acid Sequence; Cysteine; Glyceraldehyde-3-Phosphate Dehydrogenases; Guanidine; Guanidines; Histidine; Hydrogen-Ion Concentration; Kinetics; L-Lactate Dehydrogenase; Luminescent Measurements; Molecular Sequence Data; Peptides; Protein Denaturation; Proteins; Tryptophan; Tyrosine; Viscosity | 1995 |
Quantification of cysteinyl sulfhydryl residues in peptides and proteins by ESI-MS or MALDI-MS.
Topics: Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Guanidine; Guanidines; Indicators and Reagents; Mass Spectrometry; Peptides; Plant Proteins; Plants; Pyridines; Sulfhydryl Compounds | 1995 |
Conformational stability of Cys45-alkylated and hydrogen peroxide-oxidised glutathione S-transferase.
Topics: Alkylation; Animals; Cysteine; Dithiothreitol; Enzyme Stability; Glutathione; Glutathione Transferase; Guanidine; Guanidines; Hydrogen Peroxide; Naphthalenesulfonates; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Structure-Activity Relationship; Swine; Thermodynamics | 1995 |
Hexose phosphate binding sites of fructose 6-phosphate,2-kinase:fructose 2,6-bisphosphatase.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cysteine; Guanidine; Guanidines; Hexosephosphates; Kinetics; Molecular Sequence Data; Multienzyme Complexes; Mutagenesis, Site-Directed; Phosphofructokinase-2; Phosphoric Monoester Hydrolases; Phosphotransferases; Protein Denaturation; Rats; Sequence Alignment; Sequence Homology, Amino Acid; Structure-Activity Relationship | 1995 |
Role of the C-terminal helix in the folding and stability of yeast phosphoglycerate kinase.
Topics: Circular Dichroism; Cysteine; Guanidine; Guanidines; Magnetic Resonance Spectroscopy; Molecular Weight; Mutagenesis, Site-Directed; Peptide Fragments; Phosphoglycerate Kinase; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Saccharomyces cerevisiae; Structure-Activity Relationship; Thermodynamics | 1995 |
Cysteines of chloroplast NADP-malate dehydrogenase form mixed disulfides.
Topics: Carbon Radioisotopes; Chloroplasts; Cysteine; Disulfides; Enzyme Activation; Glutathione; Guanidine; Guanidines; Isotope Labeling; Malate Dehydrogenase; Malate Dehydrogenase (NADP+); Models, Chemical; Oxidation-Reduction; Peptide Fragments; Pisum sativum; Serine Endopeptidases; Spinacia oleracea; Vegetables | 1994 |
Two-state transition between molten globule and unfolded states of acetylcholinesterase as monitored by electron paramagnetic resonance spectroscopy.
Topics: Acetylcholinesterase; Amino Acid Sequence; Animals; Chromatography, Affinity; Circular Dichroism; Cysteine; Electric Organ; Electron Spin Resonance Spectroscopy; Guanidine; Guanidines; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Torpedo | 1994 |
Role of the Cys 2-Cys 10 disulfide bond for the structure, stability, and folding kinetics of ribonuclease T1.
Topics: Amino Acid Isomerases; Amino Acid Sequence; Carrier Proteins; Circular Dichroism; Cysteine; Disulfides; Enzyme Stability; Guanidine; Guanidines; Kinetics; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Molecular Structure; Peptidylprolyl Isomerase; Protein Denaturation; Protein Folding; Ribonuclease T1; Thermodynamics | 1994 |
Intestinal fatty acid binding protein: characterization of mutant proteins containing inserted cysteine residues.
Topics: Animals; Carrier Proteins; Cysteine; Disulfides; Dithionitrobenzoic Acid; Drug Stability; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Mutagenesis, Site-Directed; Neoplasm Proteins; Nerve Tissue Proteins; Protein Denaturation; Protein Structure, Secondary; Pyridines; Rats; Spectrometry, Fluorescence; Sulfhydryl Compounds | 1993 |
Hysteresis in the triple helix-coil transition of type III collagen.
Topics: Amino Acid Sequence; Animals; Calorimetry, Differential Scanning; Cattle; Cells, Cultured; Collagen; Cysteine; Ehlers-Danlos Syndrome; Fetus; Fibroblasts; Guanidine; Guanidines; Humans; Kinetics; Methionine; Molecular Sequence Data; Proline; Protein Denaturation; Protein Folding; Skin; Sulfur Radioisotopes; Thermodynamics; Tritium | 1993 |
Probing weakly polar interactions in cytochrome c.
Topics: Cysteine; Cytochrome c Group; Cytochromes c; Guanidine; Guanidines; Magnetic Resonance Spectroscopy; Methionine; Models, Molecular; Mutation; Phenylalanine; Protein Conformation; Protein Denaturation; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Thermodynamics; Tyrosine | 1993 |
A recombinant extracellular domain of the thyrotropin (TSH) receptor binds TSH in the absence of membranes.
Topics: Amino Acid Sequence; Animals; Antibodies; Baculoviridae; Binding Sites; Binding, Competitive; Cell Membrane; Chromatography, High Pressure Liquid; Cysteine; Cystine; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Follicle Stimulating Hormone; Guanidine; Guanidines; Humans; Immunoblotting; Luteinizing Hormone; Molecular Sequence Data; Molecular Weight; Protein Folding; Receptors, Thyrotropin; Recombinant Proteins; Thyrotropin; Transfection | 1994 |
Expression and characterization of recombinant human and rat liver 6-pyruvoyl tetrahydropterin synthase. Modified cysteine residues inhibit the enzyme activity.
Topics: Alcohol Oxidoreductases; Animals; Base Sequence; Chromatography, Gel; Cloning, Molecular; Cysteine; DNA Primers; Escherichia coli; Ethylmaleimide; Factor Xa; Gene Expression; Genetic Vectors; Guanidine; Guanidines; Humans; Kinetics; Liver; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphorus-Oxygen Lyases; Pyridines; Rats; Recombinant Proteins; Restriction Mapping | 1994 |
Characterization of folding intermediates of human carbonic anhydrase II: probing substructure by chemical labeling of SH groups introduced by site-directed mutagenesis.
Topics: Alkylation; Carbonic Anhydrases; Circular Dichroism; Cysteine; Enzyme Stability; Guanidine; Guanidines; Humans; Mutagenesis, Site-Directed; Protein Folding; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Thermodynamics | 1993 |
Influence of primary sequence transpositions on the folding pathways of ribonuclease T1.
Topics: Amino Acid Sequence; Calorimetry; Cloning, Molecular; Cysteine; Disulfides; Escherichia coli; Glycine; Guanidine; Guanidines; Kinetics; Molecular Sequence Data; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Ribonuclease T1; Spectrometry, Fluorescence; Structure-Activity Relationship; Thermodynamics; Time Factors | 1996 |
Human neurotrophin-3: a one-step peptide mapping method and complete disulfide characterization of the recombinant protein.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Chymotrypsin; Cysteine; Disulfides; Dithiothreitol; Escherichia coli; Guanidine; Guanidines; Humans; Molecular Sequence Data; Nerve Growth Factors; Neurotrophin 3; Peptide Fragments; Peptide Mapping; Protein Denaturation; Recombinant Proteins; Thermolysin | 1996 |
Denaturants can accelerate folding rates in a class of globular proteins.
Topics: Aprotinin; Cysteine; Disulfides; Guanidine; Guanidines; Kinetics; Models, Molecular; Monte Carlo Method; Protein Denaturation; Protein Folding; Ribonuclease T1; Thermodynamics; Urea | 1996 |
Two partially unfolded states of Torpedo californica acetylcholinesterase.
Topics: Acetylcholinesterase; Animals; Calorimetry, Differential Scanning; Chemical Phenomena; Chemistry, Physical; Cholinesterase Inhibitors; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electric Organ; Electron Spin Resonance Spectroscopy; Guanidine; Guanidines; Macromolecular Substances; Protein Folding; Spectrometry, Fluorescence; Sulfhydryl Reagents; Torpedo | 1996 |
Refolding of triosephosphate isomerase in low-water media investigated by fluorescence resonance energy transfer.
Topics: Animals; Cetrimonium; Cetrimonium Compounds; Cysteine; Detergents; Fluorescein-5-isothiocyanate; Guanidine; Guanidines; Hexanols; Micelles; Muscle, Skeletal; Naphthalenesulfonates; Octanes; Protein Conformation; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Triose-Phosphate Isomerase | 1996 |
Destabilizing interactions between the partners of a bifunctional fusion protein.
Topics: ATP-Binding Cassette Transporters; Bacteriophage M13; Carrier Proteins; Chromatography, Gel; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Guanidine; Guanidines; Kinetics; Maltose-Binding Proteins; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Mutation; Oxidation-Reduction; Periplasmic Binding Proteins; Protein Denaturation; Recombinant Fusion Proteins; Ultracentrifugation | 1996 |
Kinetics and motional dynamics of spin-labeled yeast iso-1-cytochrome c: 1. Stopped-flow electron paramagnetic resonance as a probe for protein folding/unfolding of the C-terminal helix spin-labeled at cysteine 102.
Topics: Circular Dichroism; Cysteine; Cytochrome c Group; Cytochromes c; Electron Spin Resonance Spectroscopy; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Mesylates; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spectrophotometry; Spin Labels; Temperature; Thermodynamics | 1997 |
Cysteine reactivity in Thermoanaerobacter brockii alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Bacteria, Anaerobic; Cysteine; Disulfides; Dithiothreitol; Electron Spin Resonance Spectroscopy; Gram-Positive Asporogenous Rods, Irregular; Guanidine; Guanidines; Iodoacetates; Iodoacetic Acid; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Spin Labels; Thermodynamics | 1997 |
Importance of phospholipid in the folding and conformation of phosphatidylinositol transfer protein: comparison of apo and holo species.
Topics: Carrier Proteins; Circular Dichroism; Cysteine; Guanidine; Guanidines; Isomerism; Membrane Proteins; Oxidation-Reduction; Phospholipid Transfer Proteins; Protein Conformation; Protein Denaturation; Protein Folding | 1997 |
Mass spectrometric determination of the cleavage sites in Escherichia coli dihydroorotase induced by a cysteine-specific reagent.
Topics: Binding Sites; Cysteine; Dihydroorotase; Dithionitrobenzoic Acid; Escherichia coli; Guanidine; Kinetics; Macromolecular Substances; Peptide Fragments; Spectrometry, Mass, Secondary Ion; Sulfhydryl Reagents; Thiocyanates | 1997 |
Pyrene excimer fluorescence as a proximity probe for investigation of residual structure in the unfolded state of human carbonic anhydrase II.
Topics: Carbonic Anhydrases; Circular Dichroism; Computer Simulation; Cysteine; Fluorescent Dyes; Guanidine; Humans; Iodoacetamide; Models, Molecular; Protein Conformation; Protein Denaturation; Pyrenes; Spectrometry, Fluorescence | 1997 |
Insight into the conformation of protein folding intermediate(s) trapped by GroEL.
Topics: Aspartate Aminotransferases; Chaperonin 10; Chaperonin 60; Cysteine; Endopeptidase K; Endopeptidases; Guanidine; Mitochondria; Models, Molecular; Molecular Chaperones; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Folding; Sulfhydryl Reagents; Trypsin | 1998 |
Two conformational states of beta-lactamase bound to GroEL: a biophysical characterization.
Topics: Alanine; beta-Lactamases; Chaperonin 60; Cysteine; Guanidine; Kinetics; Macromolecular Substances; Mass Spectrometry; Protein Conformation; Protein Denaturation; Protein Folding; Temperature | 1998 |
Unfolding of acrylodan-labeled human serum albumin probed by steady-state and time-resolved fluorescence methods.
Topics: 2-Naphthylamine; Binding Sites; Cysteine; Energy Transfer; Fluorescent Dyes; Guanidine; Humans; Kinetics; Models, Chemical; Protein Denaturation; Protein Folding; Regression Analysis; Salicylates; Serum Albumin; Spectrometry, Fluorescence; Thermodynamics; Time Factors | 1998 |
Sunlight mutagenesis: changes in mutational specificity during the irradiation of phage M13mp2.
Topics: Bacteriophage M13; Base Sequence; Cysteine; DNA; Dose-Response Relationship, Drug; Escherichia coli; Guanidine; Guanosine; Molecular Sequence Data; Mutagenicity Tests; Sunlight; Time Factors; Ultraviolet Rays | 1999 |
Evidence for kinetic intermediate states during the refolding of GdnHCl-denatured MM-creatine kinase. Characterization of a trapped monomeric species.
Topics: Anilino Naphthalenesulfonates; Animals; Chemical Phenomena; Chemistry, Physical; Chromatography, Gel; Creatine Kinase; Cysteine; Enzyme Activation; Guanidine; Isoenzymes; Kinetics; Models, Chemical; Protein Binding; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Titrimetry; Tryptophan; Tyrosine | 1998 |
Unfolding of Plasmodium falciparum triosephosphate isomerase in urea and guanidinium chloride: evidence for a novel disulfide exchange reaction in a covalently cross-linked mutant.
Topics: Animals; Chromatography, Gel; Circular Dichroism; Cross-Linking Reagents; Cysteine; Disulfides; Guanidine; Models, Molecular; Mutagenesis, Site-Directed; Plasmodium falciparum; Protein Conformation; Protein Denaturation; Recombinant Proteins; Spectrometry, Fluorescence; Triose-Phosphate Isomerase; Tryptophan; Tyrosine; Urea | 1999 |
A single disulfide bridge (Cys182-Cys264) is crucial for alpha-dystroglycan N-terminal domain stability.
Topics: Amino Acid Sequence; Cysteine; Cytoskeletal Proteins; Disulfides; Dystroglycans; Guanidine; Membrane Glycoproteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Point Mutation; Protein Denaturation; Receptors, Laminin; Recombinant Proteins | 1998 |
Stability of the residual structure in unfolded BPTI in different conditions of temperature and solvent composition measured by disulphide kinetics and double mutant cycle analysis.
Topics: Animals; Aprotinin; Cattle; Cysteine; Disulfides; Glutathione Disulfide; Guanidine; Kinetics; Models, Molecular; Mutation; Osmolar Concentration; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Solvents; Temperature | 1999 |
The fMet-tRNA binding domain of translational initiation factor IF2: role and environment of its two Cys residues.
Topics: Binding Sites; Cysteine; Geobacillus stearothermophilus; Guanidine; Mutagenesis, Site-Directed; Peptide Initiation Factors; Prokaryotic Initiation Factor-2; Protein Conformation; Protein Denaturation; RNA, Transfer, Met; Spectrum Analysis, Raman | 1999 |
The cooperativity of burst phase reactions explored.
Topics: Bacteriophage T4; Cysteine; Escherichia coli; Guanidine; Hydrogen; Kinetics; Models, Theoretical; Muramidase; Mutation; Protein Denaturation; Protein Folding; Ribonuclease H; Thermodynamics; Time Factors; Water | 1999 |
Solid-state synthesis and mechanical unfolding of polymers of T4 lysozyme.
Topics: Bacteriophage T4; Cysteine; Electrophoresis, Capillary; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Guanidine; Microscopy, Atomic Force; Models, Molecular; Molecular Sequence Data; Muramidase; Oxygen; Polymers; Protein Folding; Stress, Mechanical | 2000 |
The transition state in the folding-unfolding reaction of four species of three-disulfide variant of hen lysozyme: the role of each disulfide bridge.
Topics: Amino Acid Substitution; Animals; Binding Sites; Calorimetry; Chickens; Circular Dichroism; Cysteine; Disulfides; Enzyme Stability; Female; Fluorescence; Genetic Variation; Guanidine; Kinetics; Methionine; Models, Molecular; Muramidase; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Tertiary; Recombinant Proteins; Thermodynamics; Titrimetry; Trisaccharides | 2000 |
Unfolding thermodynamics of the tetrameric chaperone, SecB.
Topics: Bacterial Proteins; Calorimetry, Differential Scanning; Cysteine; Escherichia coli; Guanidine; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Molecular Chaperones; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry; Temperature; Thermodynamics | 2000 |
The cysteine-rich protein A from Helicobacter pylori is a beta-lactamase.
Topics: Amino Acid Sequence; Bacterial Proteins; beta-Lactamases; Cysteine; Disulfides; Guanidine; Helicobacter pylori; Kinetics; Molecular Sequence Data; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Sorting Signals; Recombinant Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity | 2000 |
Hierarchical unfolding of the alpha-lactalbumin molten globule: presence of a compact intermediate without a unique tertiary fold.
Topics: Amino Acid Substitution; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Disulfides; Dose-Response Relationship, Drug; Guanidine; Humans; Isomerism; Lactalbumin; Models, Molecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Urea | 2000 |
Evidence for tryptophan in proximity to histidine and cysteine as essential to the active site of an alkaline protease.
Topics: Affinity Labels; Binding Sites; Chromatography, DEAE-Cellulose; Conidiobolus; Cysteine; Endopeptidases; Energy Transfer; Fluoresceins; Guanidine; Histidine; Kinetics; Spectrometry, Fluorescence; Tryptophan; Ultrafiltration | 2000 |
Fluorescence energy transfer indicates similar transient and equilibrium intermediates in staphylococcal nuclease folding.
Topics: Amino Acid Substitution; Anilino Naphthalenesulfonates; Anions; Circular Dichroism; Cysteine; Energy Transfer; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Models, Molecular; Pliability; Proline; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Thermodynamics; Titrimetry; Tryptophan; Urea | 2000 |
Thermodynamic characterization of mutants of human fibroblast growth factor 1 with an increased physiological half-life.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Cysteine; Fibroblast Growth Factor 1; Guanidine; Half-Life; Humans; Mutagenesis; Mutation; Protein Denaturation; Protein Folding; Serine; Thermodynamics | 2000 |
Loss of either of the two heme-binding cysteines from a class I c-type cytochrome has a surprisingly small effect on physicochemical properties.
Topics: Amino Acid Sequence; Amino Acid Substitution; Bacteria, Aerobic; Base Sequence; Binding Sites; Cloning, Molecular; Cysteine; Cytochrome c Group; Escherichia coli; Guanidine; Heme; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Protein Denaturation; Protein Folding; Recombinant Proteins; Templates, Genetic | 2000 |
Structural and functional consequences of removal of the interdomain disulfide bridge from the isolated C-lobe of ovotransferrin.
Topics: Conalbumin; Crystallography, X-Ray; Cysteine; Diphosphates; Disulfides; Guanidine; Iron; Kinetics; Models, Chemical; Peptides; Protein Conformation; Urea | 2000 |
Protein compactness measured by fluorescence resonance energy transfer. Human carbonic anhydrase ii is considerably expanded by the interaction of GroEL.
Topics: Amino Acid Sequence; Amino Acid Substitution; Carbonic Anhydrases; Chaperonin 60; Cysteine; Energy Transfer; Fluorescent Dyes; Guanidine; Humans; Models, Molecular; Naphthalenesulfonates; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence | 2001 |
Pressure-induced perturbation on the active site of beta-amylase monitored from the sulfhydryl reaction.
Topics: beta-Amylase; Binding Sites; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Guanidine; Pressure; Protein Conformation; Protein Folding; Solanaceae; Spectrometry, Fluorescence; Sulfhydryl Reagents; Thermodynamics; Thiocyanates | 2001 |
Identification, substrate specificity, and inhibition of the Streptococcus pneumoniae beta-ketoacyl-acyl carrier protein synthase III (FabH).
Topics: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; Amino Acid Sequence; Anti-Bacterial Agents; Asparagine; Catalysis; Chromatography; Circular Dichroism; Cloning, Molecular; Cysteine; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Guanidine; Histidine; Indoles; Inhibitory Concentration 50; Isoelectric Focusing; Kinetics; Models, Chemical; Molecular Sequence Data; Sequence Homology, Amino Acid; Streptococcus pneumoniae; Substrate Specificity; Thiophenes; Ultraviolet Rays | 2001 |
On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobin.
Topics: Amides; Animals; Circular Dichroism; Cysteine; Disulfides; Dithionitrobenzoic Acid; Guanidine; Hydrogen; Kinetics; Methyl Methanesulfonate; Models, Molecular; Mutation; Myoglobin; Protein Conformation; Protein Folding; Sulfhydryl Compounds; Thermodynamics; Whales | 2001 |
Effects of fluorescent probe structure on the dynamics at cysteine-34 within bovine serum albumin: evidence for probe-dependent modulation of the cybotactic region.
Topics: Animals; Boron Compounds; Cattle; Cysteine; Fluorescence Polarization; Fluorescent Dyes; Guanidine; Hot Temperature; In Vitro Techniques; Kinetics; Models, Molecular; Protein Binding; Protein Denaturation; Serum Albumin, Bovine; Spectrometry, Fluorescence | 2001 |
Engineering of an intersubunit disulfide bridge in the iron-superoxide dismutase of Mycobacterium tuberculosis.
Topics: Cloning, Molecular; Crystallography, X-Ray; Cysteine; Dimerization; Disulfides; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanine; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Mycobacterium tuberculosis; Protein Denaturation; Protein Structure, Quaternary; Serine; Superoxide Dismutase; Thermodynamics | 2002 |
The crystal structure of Helicobacter pylori cysteine-rich protein B reveals a novel fold for a penicillin-binding protein.
Topics: Amino Acid Motifs; Amino Acid Sequence; Amino Acids; Bacterial Proteins; beta-Lactamases; Binding Sites; Carrier Proteins; Crystallography, X-Ray; Cysteine; Disulfides; Dose-Response Relationship, Drug; Guanidine; Helicobacter pylori; Hexosyltransferases; Hydrolysis; Kinetics; Models, Molecular; Molecular Sequence Data; Muramoylpentapeptide Carboxypeptidase; Open Reading Frames; Penicillin-Binding Proteins; Peptidyl Transferases; Protein Binding; Protein Folding; Repetitive Sequences, Amino Acid; Sequence Homology, Amino Acid; Water | 2002 |
Unfolding rates of barstar determined in native and low denaturant conditions indicate the presence of intermediates.
Topics: Bacterial Proteins; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Enzyme Inhibitors; Guanidine; Kinetics; Protein Denaturation; Protein Folding; Solutions; Spectrometry, Fluorescence; Sulfhydryl Reagents; Urea | 2002 |
Intramolecular versus intermolecular disulfide bonds in prion proteins.
Topics: Animals; Cell-Free System; Cricetinae; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Guanidine; Hydrogen-Ion Concentration; Immunoblotting; Oxygen; Prions; Protein Binding; Protein Conformation; Sulfhydryl Compounds | 2002 |
Conformational changes at the active site of adenylate kinase detected using a fluorescent probe and monoclonal antibody binding.
Topics: Adenylate Kinase; Animals; Antibodies, Monoclonal; Binding Sites; Cysteine; Fluorescence; Fluorescent Dyes; Guanidine; Protein Binding; Protein Conformation; Protein Denaturation; Rabbits; Time Factors; Urea | 2002 |
Covalent modification of epithelial fatty acid-binding protein by 4-hydroxynonenal in vitro and in vivo. Evidence for a role in antioxidant biology.
Topics: Aldehydes; Amino Acid Sequence; Amino Acid Substitution; Animals; Antioxidants; Carrier Proteins; Cysteine; Epithelial Cells; Escherichia coli; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Guanidine; Hydrogen-Ion Concentration; Kinetics; Mice; Mice, Knockout; Molecular Sequence Data; Mutagenesis, Site-Directed; Neoplasm Proteins; Nerve Tissue Proteins; Oxidative Stress; Peptide Fragments; Protein Denaturation; Recombinant Proteins; Thermodynamics | 2002 |
Renaturation of human proinsulin--a study on refolding and conversion to insulin.
Topics: Carboxypeptidase B; Carboxypeptidases; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Cystine; Enzyme-Linked Immunosorbent Assay; Guanidine; Humans; Hydrogen-Ion Concentration; Insulin; Oxidation-Reduction; Proinsulin; Protein Denaturation; Protein Renaturation; Protein Structure, Secondary; Recombinant Proteins; Temperature; Trypsin; Urea | 2002 |
Complete disulfide bond assignment of a recombinant immunoglobulin G4 monoclonal antibody.
Topics: Amino Acid Sequence; Animals; Antibodies, Monoclonal; CHO Cells; Chromatography, High Pressure Liquid; Cricetinae; Cysteine; Disulfides; Ethylmaleimide; Guanidine; Humans; Immunoglobulin G; Metalloendopeptidases; Molecular Sequence Data; Peptides; Recombinant Proteins; Sequence Analysis, Protein; Spectrometry, Mass, Electrospray Ionization | 2002 |
Structural and thermodynamic studies on a salt-bridge triad in the NADP-binding domain of glutamate dehydrogenase from Thermotoga maritima: cooperativity and electrostatic contribution to stability.
Topics: Alanine; Arginine; Binding Sites; Crystallography, X-Ray; Cysteine; Enzyme Stability; Glutamate Dehydrogenase; Glutamic Acid; Guanidine; Lysine; Mutagenesis, Site-Directed; NADP; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Recombinant Proteins; Salts; Serine; Static Electricity; Structure-Activity Relationship; Thermodynamics; Thermotoga maritima; Urea | 2002 |
Dramatic stabilization of the native state of human carbonic anhydrase II by an engineered disulfide bond.
Topics: Alanine; Amino Acid Substitution; Carbonic Anhydrase II; Circular Dichroism; Cysteine; Disulfides; Enzyme Activation; Enzyme Stability; Genetic Variation; Guanidine; Humans; Leucine; Mutagenesis, Site-Directed; Neisseria gonorrhoeae; Protein Denaturation; Protein Folding | 2002 |
The molecular neighborhood of subunit 8 of yeast mitochondrial F1F0-ATP synthase probed by cysteine scanning mutagenesis and chemical modification.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Cross-Linking Reagents; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Fungal Proteins; Guanidine; Hydrolysis; Immunoblotting; Lipid Bilayers; Mitochondria; Models, Biological; Mutagenesis, Site-Directed; Mutation; Protein Binding; Protein Structure, Tertiary; Proton-Translocating ATPases; Temperature; Time Factors; Valine | 2003 |
Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolA.
Topics: Amino Acid Sequence; Anisotropy; Circular Dichroism; Colicins; Cysteine; Escherichia coli; Escherichia coli Proteins; Fluorescence Resonance Energy Transfer; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Surface Plasmon Resonance; Time Factors; Tryptophan; Water | 2003 |
Equilibrium unfolding of dimeric human prostatic acid phosphatase involves an inactive monomeric intermediate.
Topics: Acid Phosphatase; Catalysis; Catalytic Domain; Chromatography; Chromatography, High Pressure Liquid; Cysteine; Dimerization; Dose-Response Relationship, Drug; Guanidine; Humans; Kinetics; Male; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Protein Tyrosine Phosphatases; Spectrometry, Fluorescence; Thermodynamics; Time Factors; Tryptophan | 2003 |
Functional characterization of ERp18, a new endoplasmic reticulum-located thioredoxin superfamily member.
Topics: Amino Acid Sequence; Animals; Binding Sites; Catalysis; Circular Dichroism; COS Cells; Cysteine; Disulfides; Endoplasmic Reticulum; Escherichia coli; Gene Expression; Guanidine; Humans; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Oxidation-Reduction; Polymerase Chain Reaction; Protein Conformation; Protein Denaturation; Protein Disulfide Reductase (Glutathione); Protein Disulfide-Isomerases; Recombinant Proteins; Sequence Alignment; Spectrometry, Fluorescence; Structure-Activity Relationship; Sulfhydryl Compounds; Thermodynamics; Thioredoxins; Transfection | 2003 |
The multiple site binding of carboxypeptidase Y inhibitor (IC) to the cognate proteinase. Implications for the biological roles of the phosphatidylethanolamine-binding protein.
Topics: Amino Acid Sequence; Androgen-Binding Protein; Animals; Binding Sites; Brain; Carboxypeptidases; Carrier Proteins; Catalysis; Cathepsin A; Chromatography, Gel; Cross-Linking Reagents; Cysteine; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Guanidine; Kinetics; Mice; Molecular Sequence Data; Parasympathomimetics; Phosphatidylethanolamines; Phospholipid Transfer Proteins; Protein Binding; Protein Structure, Tertiary; Saccharomyces cerevisiae; Sequence Homology, Amino Acid; Serine; Time Factors; Trypsin | 2003 |
Characterizing the unstructured intermediates in oxidative folding.
Topics: Chromatography, High Pressure Liquid; Cysteine; Disulfides; Guanidine; Hydrogen-Ion Concentration; Isomerism; Models, Biological; Organoplatinum Compounds; Oxidation-Reduction; Protein Denaturation; Protein Folding; Ribonuclease, Pancreatic | 2003 |
ISOCITRIC-DEHYDROGENASE OF RABBIT RETICULOCYTES AND ERYTHROCYTES. II. THE INFLUENCE OF PHYSICAL AND CHEMICAL AGENTS ON THE LIBERATION OF THE ENZYME; THE MAXIMAL ACTIVITY OF THE ENZYME IN THE CELL.
Topics: Animals; Cold Temperature; Cysteine; Erythrocytes; Glutathione; Guanidine; Guanidines; Isocitrate Dehydrogenase; Lagomorpha; Oxidoreductases; Physical Examination; Rabbits; Research; Reticulocytes; Sucrose; Urea | 1963 |
[STUDIES ON HABU-SNAKE VENOM].
Topics: Animals; Crotalid Venoms; Cysteine; Ecchymosis; Formaldehyde; Glucuronates; Guanidine; Guanidines; Hemolysis; Injections, Intraperitoneal; Injections, Subcutaneous; Lagomorpha; Mice; Pharmacology; Rabbits; Research; Salicylates; Snake Venoms; Snakes; Sulfates; Thioglycolates; Toxicology; Trimeresurus; Urea; Venoms | 1963 |
Destabilizing mutations promote membrane protein misfolding.
Topics: Amino Acid Sequence; Amino Acid Substitution; Cysteine; Diacylglycerol Kinase; Enzyme Stability; Escherichia coli Proteins; Genetic Variation; Guanidine; Kinetics; Liposomes; Membrane Proteins; Micelles; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphatidylcholines; Protein Denaturation; Protein Folding; Thermodynamics | 2004 |
Folding of an abridged beta-lactamase.
Topics: Bacillus; Bacterial Proteins; beta-Lactamases; Circular Dichroism; Cysteine; Guanidine; Isoenzymes; Kinetics; Mutagenesis, Site-Directed; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Sequence Deletion; Spectrometry, Fluorescence | 2004 |
Analysis of the early stage of the folding process of reduced lysozyme using all lysozyme variants containing a pair of cysteines.
Topics: Animals; Chickens; Cysteine; Disulfides; Entropy; Genetic Variation; Guanidine; Muramidase; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Denaturation; Protein Folding; Recombinant Proteins; Solutions; Water | 2004 |
Denaturant-assisted formation of a stabilizing disulfide bridge from engineered cysteines in nonideal conformations.
Topics: Alanine; Carbonic Anhydrase II; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Entropy; Enzyme Stability; Guanidine; Humans; Leucine; Models, Molecular; Mutagenesis, Site-Directed; Neisseria gonorrhoeae; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Software; Structure-Activity Relationship | 2005 |
Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coli.
Topics: Allosteric Regulation; Cysteine; Dimerization; Enzyme Inhibitors; Enzyme Stability; Escherichia coli; Fructosephosphates; Guanidine; Kinetics; Mutation; Phosphofructokinase-2; Protein Denaturation; Protein Folding; Protein Subunits; Substrate Specificity | 2005 |
Role of the disulphide bridge in folding, stability and function of porcine odorant binding protein: spectroscopic equilibrium studies on C63A/C155A double mutant.
Topics: Animals; Circular Dichroism; Cysteine; Disulfides; Guanidine; Ligands; Mutation; Protein Denaturation; Protein Folding; Protein Renaturation; Receptors, Odorant; Spectrometry, Fluorescence; Swine; Thermodynamics | 2005 |
Unfolding and breakdown of insulin in the presence of endogenous thiols.
Topics: Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Guanidine; Hot Temperature; Humans; Hydrogen-Ion Concentration; Insulin; Kinetics; Mass Spectrometry; Molecular Conformation; Pancreas; Protein Conformation; Protein Denaturation; Protein Folding; Sulfhydryl Compounds; Temperature; Time Factors | 2005 |
Methanococcus vannielii selenium-binding protein (SeBP): chemical reactivity of recombinant SeBP produced in Escherichia coli.
Topics: Calorimetry, Differential Scanning; Cysteine; Electrophoresis; Escherichia coli; Glutathione; Guanidine; Hydrogen-Ion Concentration; Methanococcus; Phosphines; Protein Binding; Protein Subunits; Recombinant Proteins; Selenium-Binding Proteins; Sodium Selenite; Spectrophotometry, Atomic | 2005 |
Aggregation of granulocyte-colony stimulating factor in vitro involves a conformationally altered monomeric state.
Topics: Biophysical Phenomena; Biophysics; Circular Dichroism; Cysteine; Fluorescence; Granulocyte Colony-Stimulating Factor; Guanidine; Humans; Hydrogen-Ion Concentration; Models, Molecular; Protein Conformation; Protein Denaturation; Recombinant Proteins; Tryptophan | 2005 |
Cloning, expression, purification, and characterization of Vibrio cholerae transcriptional activator, HlyU.
Topics: Bacterial Proteins; Chromatography, Gel; Circular Dichroism; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Guanidine; Models, Genetic; Protein Folding; Solvents; Spectrometry, Fluorescence; Trans-Activators; Transcription Factors; Transcription, Genetic; Vibrio cholerae | 2006 |
Substitution of serine for non-disulphide-bond-forming cysteine in grass carp (Ctenopharygodon idellus) growth hormone improves in vitro oxidative renaturation.
Topics: Amino Acid Substitution; Animals; Carps; Cysteine; Gene Expression Profiling; Growth Hormone; Guanidine; Hydrogen-Ion Concentration; In Vitro Techniques; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Folding; Protein Renaturation; Recombinant Proteins; Serine; Time Factors | 2006 |
Characterization of intra-molecular distances and site-specific dynamics in chemically unfolded barstar: evidence for denaturant-dependent non-random structure.
Topics: Bacterial Proteins; Cysteine; Fluorescence Resonance Energy Transfer; Guanidine; Models, Molecular; Molecular Structure; Mutation; Nitrobenzoates; Protein Conformation; Protein Denaturation; Sulfhydryl Compounds; Urea | 2006 |
Conformational stability of secretory leucocyte protease inhibitor: a protein with no hydrophobic core and very little secondary structure.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithiothreitol; Guanidine; Guanidines; Humans; Hydrophobic and Hydrophilic Interactions; Isothiocyanates; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteinase Inhibitory Proteins, Secretory; Proteins; Recombinant Proteins; Secretory Leukocyte Peptidase Inhibitor; Thermodynamics; Urea | 2006 |
The location of an engineered inter-subunit disulfide bond in factor for inversion stimulation (FIS) affects the denaturation pathway and cooperativity.
Topics: Circular Dichroism; Crystallography, X-Ray; Cysteine; Disulfides; DNA; Factor For Inversion Stimulation Protein; Fluorescence; Guanidine; Hydrogen Bonding; Hydrogen-Ion Concentration; Models, Molecular; Mutation; Oxidation-Reduction; Protein Binding; Protein Denaturation; Protein Engineering; Protein Subunits; Serine; Structure-Activity Relationship; Urea; Valine | 2006 |
Site-directed mutagenesis indicates an important role of cysteines 76 and 181 in the catalysis of hydantoin racemase from Sinorhizobium meliloti.
Topics: Amino Acid Sequence; Binding Sites; Calorimetry; Catalysis; Circular Dichroism; Cloning, Molecular; Computer Simulation; Conserved Sequence; Cysteine; Fluorescence; Guanidine; Models, Biological; Models, Molecular; Mutagenesis, Site-Directed; Mutant Proteins; Protein Binding; Protein Conformation; Protein Folding; Racemases and Epimerases; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Sinorhizobium meliloti | 2006 |
Site-specific conformational studies of prion protein (PrP) amyloid fibrils revealed two cooperative folding domains within amyloid structure.
Topics: 2-Naphthylamine; Amyloid; Animals; Cysteine; Disulfides; Fluorescent Dyes; Guanidine; Kinetics; Mice; Molecular Conformation; Prion Proteins; Prions; Protein Conformation; Protein Denaturation; Protein Folding; Thermodynamics | 2007 |
Mutations of Cys-17 and Ala-271 in the human histamine H2 receptor determine the species selectivity of guanidine-type agonists and increase constitutive activity.
Topics: Adenylyl Cyclases; Alanine; Animals; Baculoviridae; Cell Line; Cell Membrane; Cysteine; GTP Phosphohydrolases; GTP-Binding Protein alpha Subunits, Gs; Guanidine; Guanosine Triphosphate; Guinea Pigs; Histamine; Histamine Agonists; Histamine H2 Antagonists; Humans; Mutation, Missense; Receptors, Histamine H2; Recombinant Fusion Proteins; Species Specificity; Spodoptera; Transfection | 2007 |
Immobilization of malarial (Plasmodium falciparum) dihydrofolate reductase for the selection of tight-binding inhibitors from combinatorial library.
Topics: Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Chromatography, High Pressure Liquid; Combinatorial Chemistry Techniques; Cysteine; Enzymes, Immobilized; Folic Acid Antagonists; Guanidine; Ligands; Mass Spectrometry; Molecular Sequence Data; Peptide Library; Plasmodium falciparum; Reproducibility of Results; Sensitivity and Specificity; Sepharose; Sulfhydryl Compounds; Sulfides; Tetrahydrofolate Dehydrogenase | 2007 |
Roles of a short connecting disulfide bond in the stability and function of psychrophilic Shewanella violacea cytochrome c (5).
Topics: Amino Acid Sequence; Bacterial Proteins; Calorimetry; Circular Dichroism; Cloning, Molecular; Cysteine; Cytochrome c Group; Disulfides; Enzyme Stability; Escherichia coli; Guanidine; Hot Temperature; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Recombinant Proteins; Sequence Analysis, Protein; Shewanella; Thermodynamics | 2007 |
Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathway.
Topics: Anilino Naphthalenesulfonates; Animals; Binding Sites; Chromatography, Gel; Circular Dichroism; Creatine Kinase; Cysteine; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; Fluorescent Dyes; Guanidine; Kinetics; Models, Biological; Muscles; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Rabbits; Spectrophotometry, Ultraviolet | 2007 |
Compact dimension of denatured states of staphylococcal nuclease.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Mutant Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Salmon; Tryptophan | 2008 |
The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor.
Topics: Amino Acid Sequence; Animals; Cell Membrane; Cloning, Molecular; Cysteine; Dose-Response Relationship, Drug; Electric Stimulation; Green Fluorescent Proteins; Guanidine; Humans; Ion Channel Gating; Ion Channels; Membrane Potentials; Models, Molecular; Molecular Conformation; Oocytes; Patch-Clamp Techniques; Protein Structure, Tertiary; Xenopus laevis | 2008 |
Kinetics of contact formation and end-to-end distance distributions of swollen disordered peptides.
Topics: Algorithms; Buffers; Circular Dichroism; Cysteine; Energy Transfer; Guanidine; Kinetics; Models, Chemical; Peptides; Protein Conformation; Protein Denaturation; Tryptophan; Urea | 2009 |
Subdomain-specific collapse of denatured staphylococcal nuclease revealed by single molecule fluorescence resonance energy transfer measurements.
Topics: Biophysics; Chemistry, Physical; Cysteine; Diffusion; Fluorescence Resonance Energy Transfer; Guanidine; Micrococcal Nuclease; Models, Statistical; Molecular Conformation; Mutation; Normal Distribution; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Proteins | 2009 |
Non-native hydrophobic interactions detected in unfolded apoflavodoxin by paramagnetic relaxation enhancement.
Topics: Amino Acid Substitution; Apoproteins; Azotobacter vinelandii; Cyclic N-Oxides; Cysteine; Dose-Response Relationship, Drug; Flavodoxin; Guanidine; Hydrophobic and Hydrophilic Interactions; Magnetics; Mesylates; Models, Molecular; Protein Binding; Protein Denaturation; Protein Structure, Secondary; Spin Labels; Temperature | 2010 |
Conformational properties of unfolded HypF-N.
Topics: Carboxyl and Carbamoyl Transferases; Cysteine; Diffusion; Escherichia coli Proteins; Guanidine; Kinetics; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Tryptophan | 2009 |
[Effects of redox state of disulfide bonds on the intrinsic fluorescence and denaturation of Trx-fused gibberellin-induced cysteine-rich protein from Gymnadnia conopsea].
Topics: Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Fluorescence; Gibberellins; Guanidine; Hydrogen-Ion Concentration; Orchidaceae; Oxidation-Reduction; Plant Proteins; Protein Conformation; Spectrometry, Fluorescence; Thermodynamics; Thioredoxins; Tryptophan | 2010 |
L-cysteine-enhanced renaturation of bioactive soluble tumor necrosis factor ligand family member LIGHT from inclusion bodies in Escherichia coli.
Topics: Alanine; Apoptosis; Biological Assay; Buffers; Cell Line, Tumor; Chromatography, High Pressure Liquid; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Humans; Inclusion Bodies; Plasmids; Protein Renaturation; Recombinant Proteins; Tumor Necrosis Factor Ligand Superfamily Member 14 | 2011 |
A synthetic approach to a peptide α-thioester from an unprotected peptide through cleavage and activation of a specific peptide bond by N-acetylguanidine.
Topics: Amino Acid Sequence; Cysteine; Glycopeptides; Guanidine; Kinetics; Molecular Sequence Data; Sulfhydryl Compounds | 2012 |
The folding pathway of glycosomal triosephosphate isomerase: structural insights into equilibrium intermediates.
Topics: Anilino Naphthalenesulfonates; Chromatography, Gel; Circular Dichroism; Cysteine; Enzyme Stability; Guanidine; Microbodies; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Folding; Protozoan Proteins; Thermodynamics; Triose-Phosphate Isomerase; Trypanosoma brucei brucei | 2012 |
Effect of ionic liquid on the native and denatured state of a protein covalently attached to a probe: solvation dynamics study.
Topics: Anisotropy; Coumarins; Cysteine; Guanidine; Humans; Ionic Liquids; Protein Conformation; Serum Albumin; Solutions; Spectrometry, Fluorescence | 2012 |
An electrochemical method for investigation of conformational flexibility of active sites of Trametes versicolor laccase based on sensitive determination of copper ion with cysteine-modified electrodes.
Topics: Catalytic Domain; Copper; Cysteine; Electrochemistry; Electrodes; Gold; Guanidine; Laccase; Protein Unfolding; Trametes | 2012 |
Intermolecular disulfide bond formation promotes immunoglobulin aggregation: investigation by fluorescence correlation spectroscopy.
Topics: Animals; Cattle; Cysteine; Disulfides; Dithionitrobenzoic Acid; Guanidine; Immunoglobulin G; Kinetics; Organophosphorus Compounds; Protein Aggregates; Protein Denaturation; Spectrometry, Fluorescence | 2015 |
The C-terminal peptide plays a role in the formation of an intermediate form during the transition between xanthine dehydrogenase and xanthine oxidase.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Crystallography, X-Ray; Cysteine; Dithiothreitol; Guanidine; Liver; Models, Biological; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Protein Conformation; Rats; Recombinant Proteins; Sequence Deletion; Sequence Homology, Amino Acid; Static Electricity; Sulfhydryl Reagents; Xanthine Dehydrogenase; Xanthine Oxidase | 2015 |
An inter-subunit disulfide bond of artemin acts as a redox switch for its chaperone-like activity.
Topics: Animals; Arthropod Proteins; Cysteine; Disulfides; Guanidine; Iron-Binding Proteins; Molecular Chaperones; Molecular Dynamics Simulation; Oxidation-Reduction; Protein Denaturation; Protein Subunits; Recombinant Proteins; RNA-Binding Proteins; Structure-Activity Relationship; Thermodynamics | 2018 |
Unravelling enhancement of antibody fragment stability - Role of format structure and cysteine modification.
Topics: Animals; Antibody Affinity; Antibody Specificity; Binding Sites, Antibody; Chickens; Complementarity Determining Regions; Cysteine; Guanidine; Immunoglobulin Fab Fragments; Immunoglobulin Fc Fragments; Immunoglobulin Heavy Chains; Mutation; Protein Conformation; Protein Stability; Single-Chain Antibodies; Structure-Activity Relationship; Temperature; Time Factors | 2019 |
In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe.
Topics: Biosensing Techniques; Circular Dichroism; Cysteine; Guanidine; Humans; Kinetics; Lactoglobulins; Protein Conformation; Protein Denaturation; Protein Folding; Protein Refolding; Protein Unfolding; Proteins; Proteostasis; Spectrometry, Fluorescence | 2021 |
Construction of Complex Macromulticyclic Peptides via Stitching with Formaldehyde and Guanidine.
Topics: Cysteine; Formaldehyde; Guanidine; Lysine; Peptides | 2022 |
Residue-Selective C-H Sulfenylation Enabled by Acid-Activated S-Acetamidomethyl Cysteine Sulfoxide with Application to One-Pot Stapling and Lipidation Sequence.
Topics: Cysteine; Guanidine; Peptides; Sulfoxides; Tyrosine | 2023 |