guanidine has been researched along with 1-anilino-8-naphthalenesulfonate in 116 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 1 (0.86) | 18.7374 |
| 1990's | 38 (32.76) | 18.2507 |
| 2000's | 53 (45.69) | 29.6817 |
| 2010's | 23 (19.83) | 24.3611 |
| 2020's | 1 (0.86) | 2.80 |
| Authors | Studies |
|---|---|
| Hryniewicz, W; Jeljaszewicz, J; Tyski, S | 1 |
| Hlodan, R; Pain, RH | 1 |
| Shastry, MC; Udgaonkar, JB | 1 |
| Prakash, V; Rajeshwara, AN | 1 |
| Gajdusek, DC; Gibbs, CJ; Roller, PP; Safar, J | 1 |
| Dobson, CM; Evans, PA; Itzhaki, LS; Radford, SE | 1 |
| Clottes, E; Couthon, F; Ebel, C; Vial, C | 1 |
| Ptitsyn, OB; Uversky, VN | 1 |
| Flores-Flores, C; Martinez-Martinez, A; Muñoz-Delgado, E; Vidal, CJ | 1 |
| Macek, P; Malavasic, M; Poklar, N; Vesnaver, G | 1 |
| Ghosh, N; Sarkar, SN | 1 |
| Löber, G; Uversky, VN; Winter, S | 1 |
| Tsou, CL; Zhang, YL; Zhou, JM | 1 |
| Ehrhard, B; Glaser, RW; Hausdorf, G; Misselwitz, R; Schneider-Mergener, J; Welfle, H; Welfle, K | 1 |
| Hamada, S; Moriyama, Y; Ogawa, K; Ohara, M; Takeda, K | 1 |
| Gopalakrishna, KN; Prakash, V; Rajeshwara, AN | 1 |
| Merrill, AR; Steer, BA | 1 |
| Ahn, JH; Lee, YP; Rhee, JS | 1 |
| Churchich, JE; Li, W | 1 |
| Fan, YX; Zhou, JM; Zhu, L | 1 |
| de Prat Gay, G; Fersht, AR; Itzhaki, LS; Ladurner, AG | 1 |
| Kim, H; Song, M | 1 |
| Llinás, M; Marqusee, S | 1 |
| Gribenko, A; Lopez, MM; Makhatadze, GI; Richardson, JM | 1 |
| Cai, H; Lei, XD; Li, J; Li, XL; Tsou, CL; Wang, CC; Yang, SL | 1 |
| Bensadoun, A; Berryman, DE; Brasaemle, DL; Hughes, LB; Mulero, JJ | 1 |
| Britton, KL; Chiaraluce, R; Consalvi, V; Engel, PC; Giangiacomo, L; Millevoi, S; Pasquo, A; Rice, DW; Stillman, TJ | 1 |
| Clottes, E; Leydier, C; Marcillat, O; Perraut, C; Vial, C | 1 |
| Jerala, R; Kroon Zitko, L; Turk, V; Virden, R; Waltho, JP; Zerovnik, E | 1 |
| Das, PK; Krupakar, J; Podder, SK | 1 |
| Clottes, E; Couthon, F; Ebel, C; Leydier, C; Marcillat, O; Vial, C | 1 |
| Fan, YX; Kihara, H; Tsou, CL; Zhou, JM | 1 |
| Malecki, J; Wasylewski, Z | 1 |
| Artigues, A; Doñate, F; Iriarte, A; Martinez-Carrion, M | 1 |
| Chang, Z; Dai, H; Gong, Y; Huang, S; Yang, H; Zheng, C | 1 |
| Inouye, K; Obata, H; Okuda, K; Senoo, K; Tanaka, A | 1 |
| Masaki, K; Mizuguchi, M; Nitta, K | 1 |
| Biktashev, AG; Khaitlina, SY; Kuznetsova, IM; Turoverov, KK; Uversky, VN; Vassilenko, KS | 1 |
| Brito, RM; Quintas, A; Saraiva, MJ | 1 |
| Agrò, AF; De Matteis, F; Di Venere, A; Mei, G; Rosato, N; Rossi, A | 1 |
| Jutila, A; Kinnunen, PK; Zhu, K | 1 |
| Eastman, P; Fink, AL; Nishimura, C; Riley, R | 1 |
| Jing, G; Yin, J | 1 |
| Hsieh, H; Kumar, TK; Samuel, D; Srimathi, T; Yu, C | 1 |
| Deshpande, VV; Pawar, SA | 1 |
| Andersen, SO | 1 |
| Andersson, D; Carlsson, U; Hammarström, P | 1 |
| Calmettes, P; Desmadril, M; Durand, D; Russo, D | 1 |
| Bläckberg, L; Fält, H; Hernell, O | 1 |
| Baranov, V; Hörstedt, P; Ippel, HJ; Lundgren, E; Olofsson, A; Wijmenga, S | 1 |
| Ou, WB; Park, YD; Yu, TW; Zhou, HM | 1 |
| Clérico, EM; Ermácora, MR | 1 |
| Nath, D; Rao, M | 1 |
| Chang, GG; Hung, HC | 1 |
| Ou, WB; Park, YD; Zhou, HM | 1 |
| Ahmad, A; Bhakuni, V; Jain, SK; Prajapati, S; Prakash, K | 1 |
| Beltramini, M; Di Muro, P; Favilla, R; Goldoni, M; Mazzini, A; Salvato, B | 1 |
| Bhattacharyya, D; Das Gupta, C; Sanyal, SC | 1 |
| Almeida, FL; Alonso, LG; García-Alai, MM; Gualfetti, P; Lapeña, AN; Nadra, AD; Prat-Gay, GD | 1 |
| Jagannadham, MV; Kundu, S; Sundd, M | 1 |
| Hong, YK; Meng, FG; Tang, H; Zhou, HM | 1 |
| Borders, CL; Brym, MJ; Chan, C; Cox, JM; Davis, CA; Edmiston, PL; Jorjorian, AD; Jourden, MJ; Snider, MJ | 1 |
| Di Venere, A; Finazzi-Agrò, A; Maccarrone, M; Mei, G; Rosato, N; Salucci, ML; van Zadelhoff, G; Veldink, G | 1 |
| Ahmad, F; Moza, B; Qureshi, SH | 1 |
| Panda, D; Santra, MK | 1 |
| Acharya, P; Rao, NM | 1 |
| Chang, GG; Lee, HJ; Lu, SW | 1 |
| Gupta, P; Khan, RH; Saleemuddin, M | 1 |
| Song, XH; Yan, SL; Zhang, YX; Zhou, HM | 1 |
| Beauregard, M; Doucet, A; McDuff, FO | 1 |
| Xie, Q; Zhou, HM | 1 |
| Aerts, T; De Deyn, P; Filée, P; Frère, JM; Joris, B; Llabrès, G; Matagne, A; Simorre, JP; Van Melckebeke, H; Vreuls, C | 1 |
| Akke, M; Miron, S; Oliveberg, M; Otzen, DE | 1 |
| Chen, Q; Huang, H; Li, X; Shi, Y; Wu, J; Xu, J; Zhang, J; Zhang, X | 1 |
| D'Auria, S; Kuznetsova, IM; Rossi, M; Scognamiglio, V; Staiano, M; Stepanenko, OV; Turoverov, KK | 1 |
| Ganea, E; Harding, JJ | 1 |
| Khan, A; Khan, RH; Naeem, A | 1 |
| Huang, GC; Li, ZY; Liu, CP; Perrett, S; Zhou, JM | 1 |
| Akiyama, S; Fujisawa, T; Ishimori, K; Kimura, T; Morishima, I; Takahashi, S; Uzawa, T | 1 |
| Beuria, TK; Mukherjee, A; Panda, D; Santra, MK | 1 |
| Aizawa, T; Demura, M; Kawano, K; Masaki, K; Matsuura, A; Mizuguchi, M; Mori, Y; Nabeshima, Y; Nitta, K; Shinoda, H; Watanabe, M | 1 |
| Ryder, AG; Togashi, DM | 1 |
| Atkinson, D; Chroni, A; Gorshkova, IN; Kan, HY; Liu, T; Zannis, VI | 1 |
| Dhanasekaran, P; Lund-Katz, S; Phillips, MC; Saito, H; Sakamoto, T; Tanaka, M; Vedhachalam, C | 1 |
| Antalík, M; Marek, J; Sedlák, E; Sprinzl, M; Valusová, E; Wolfrum, A; Zoldák, G | 1 |
| Chaudhuri, TK; Deb, JK; Jana, S | 1 |
| Fukunaga, Y; Nishimoto, E; Otosu, T; Yamashita, K; Yamashita, S | 1 |
| Ahmad, B; Haq, SK; Khan, RH; Shamim, TA | 1 |
| Dudzik, P; Kuciel, R; Mazurkiewicz, A | 1 |
| Patra, AK; Udgaonkar, JB | 1 |
| Angelaccio, S; Chiaraluce, R; Consalvi, V; Florio, R; Gianese, G; van der Oost, J; van Lieshout, JF | 1 |
| Bolivar, JM; Fernandez-Lafuente, R; Filice, M; Godoy, C; Guisan, JM; Rodrigues, RC; Volpato, G | 1 |
| O'Shaughnessy, D; Ryder, AG; Togashi, DM | 1 |
| Ahmad, B; Islam, Z; Khan, RH; Varshney, A | 1 |
| Bhattacharyya, B; Chakrabarti, P; Chakraborti, S; Chatterjee, T; Gupta, V; Joshi, P; Poddar, A; Singh, SP | 1 |
| Ahmad, E; Fatima, S; Khan, MM; Khan, RH | 1 |
| Bano, B; Khan, RH; Priyadarshini, M | 1 |
| Chen, HM; Fang, HJ; Forrest, MD; Hu, CK; Hu, HY; Tsong, TY; Wu, MC | 1 |
| Kuznetsova, IM; Povarova, OI; Turoverov, KK | 1 |
| Aatif, M; Bano, B; Rahman, S | 1 |
| Bhargava, P; Kumar, K; Roy, U | 1 |
| Abu-Omar, MM; Burgner, JW; Loaiza, A; Paul, LN; Ribbe, A; Ronau, JA; Stanciu, L | 1 |
| Chandra, V; Dave, S; Gupta, P; Mahajan, S | 1 |
| Iram, A; Naeem, A | 1 |
| Ahmad, E; Fatima, S; Khan, RH; Rabbani, G; Zaidi, N | 1 |
| Castellano, I; Graziano, G; La Cara, F; Merlino, A; Pica, A; Rossi, M; Russo Krauss, I; Sica, F | 1 |
| Garza-Ramos, G; Guzman-Luna, V | 1 |
| Ghosh, G; Mandal, DK | 1 |
| Adkar, BV; Ali, PS; Krishnan, B; Kulothungan, SR; Prajapati, RS; Sharma, L; Singh, P; Varadarajan, R | 1 |
| Mandal, DK; Sen, D | 1 |
| Gao, C; Li, L; Mu, J; Qiu, Z; Zhang, C | 1 |
| Sen, P; Yadav, R | 1 |
| Cao, X; Yu, T | 1 |
| Chaitanya, PK; Prabhu, NP | 1 |
| Honda, R; Kakuda, K; Kuwata, K; Yamaguchi, KI | 1 |
| Arreguin-Espinosa, R; Baeza-Ramírez, I; Cárdenas-Rodríguez, N; Castillo-Rodríguez, RA; Centeno-Leija, S; Cuevas-Cruz, M; Gómez-Manzo, S; González-Valdez, A; Hernández-Ochoa, B; Juárez-Cruz, MV; Marcial-Quino, J; Martínez-Rosas, V; Morales-Luna, L; Ortega-Cuellar, D; Ortiz-Ramírez, P; Pérez de la Cruz, V; Ramírez-Nava, EJ; Rocha-Ramírez, LM; Serrano-Posada, H; Sierra-Palacios, E | 1 |
116 other study(ies) available for guanidine and 1-anilino-8-naphthalenesulfonate
| Article | Year |
|---|---|
Physical and chemical properties of extracellular staphylococcal lipase.
Topics: Guanidine; Guanidines; Lipase; Oils; Osmolar Concentration; Peptide Hydrolases; Polysorbates; Sodium Chloride; Staphylococcus; Substrate Specificity; Urea | 1983 |
The folding and assembly pathway of tumour necrosis factor TNF alpha, a globular trimeric protein.
Topics: Anilino Naphthalenesulfonates; Cross-Linking Reagents; Glutaral; Guanidine; Guanidines; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan; Tumor Necrosis Factor-alpha | 1995 |
The folding mechanism of barstar: evidence for multiple pathways and multiple intermediates.
Topics: Anilino Naphthalenesulfonates; Bacterial Proteins; Fluorescent Dyes; Guanidine; Guanidines; Kinetics; Models, Chemical; Protein Folding; Recombinant Proteins; Ribonucleases; Spectrometry, Fluorescence | 1995 |
Interaction of guanidine hydrochloride and guanidine thiocyanate with wheat germ lipase.
Topics: Guanidine; Guanidines; Lipase; Protein Denaturation; Thiocyanates; Triticum | 1994 |
Scrapie amyloid (prion) protein has the conformational characteristics of an aggregated molten globule folding intermediate.
Topics: Anilino Naphthalenesulfonates; Animals; Binding Sites; Circular Dichroism; Cricetinae; Fluorescent Dyes; Guanidine; Guanidines; Hydrochloric Acid; Mesocricetus; Prions; Protein Conformation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Temperature; Tryptophan | 1994 |
Tertiary interactions in the folding pathway of hen lysozyme: kinetic studies using fluorescent probes.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Animals; Binding Sites; Binding, Competitive; Chickens; Circular Dichroism; Female; Fluorescent Dyes; Guanidine; Guanidines; Hymecromone; Iodides; Kinetics; Models, Molecular; Molecular Sequence Data; Muramidase; Oligosaccharides; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence | 1994 |
Reversible dissociation and unfolding of dimeric creatine kinase isoenzyme MM in guanidine hydrochloride and urea.
Topics: Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Creatine Kinase; Fluorescent Dyes; Guanidine; Guanidines; Isoenzymes; Muscles; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Urea | 1995 |
Further evidence on the equilibrium "pre-molten globule state": four-state guanidinium chloride-induced unfolding of carbonic anhydrase B at low temperature.
Topics: Anilino Naphthalenesulfonates; Animals; Carbonic Anhydrases; Cattle; Cold Temperature; Fluorescent Dyes; Guanidine; Guanidines; Protein Denaturation; Protein Folding; Protein Structure, Secondary | 1996 |
Conversion of acetylcholinesterase hydrophilic tetramers into amphiphilic dimers and monomers.
Topics: Acetylcholinesterase; Anilino Naphthalenesulfonates; Animals; Cattle; Centrifugation, Density Gradient; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Guanidine; Guanidines; Isoflurophate; Macromolecular Substances; Molecular Weight; Octoxynol; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet | 1996 |
Fluorescence studies of the effect of pH, guanidine hydrochloride and urea on equinatoxin II conformation.
Topics: Anilino Naphthalenesulfonates; Animals; Cnidarian Venoms; Cytotoxins; Fluorescent Dyes; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Protein Denaturation; Sea Anemones; Spectrometry, Fluorescence; Urea | 1996 |
Reversible unfolding of Escherichia coli alkaline phosphatase: active site can be reconstituted by a number of pathways.
Topics: Alkaline Phosphatase; Anilino Naphthalenesulfonates; Binding Sites; Circular Dichroism; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence | 1996 |
Use of fluorescence decay times of 8-ANS-protein complexes to study the conformational transitions in proteins which unfold through the molten globule state.
Topics: Acrylamide; Acrylamides; Anilino Naphthalenesulfonates; Animals; Cattle; Chemical Phenomena; Chemistry, Physical; Fluorescence; Fluorescent Dyes; Guanidine; Guanidines; Humans; Kinetics; Protein Conformation; Protein Folding; Proteins; Solvents; Spectrometry, Fluorescence | 1996 |
Sequential unfolding of adenylate kinase during denaturation by guanidine hydrochloride.
Topics: Adenylate Kinase; Anilino Naphthalenesulfonates; Chemical Phenomena; Chemistry, Physical; Chromatography, Gel; Circular Dichroism; Fluorescent Dyes; Guanidine; Guanidines; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectrophotometry; Tyrosine | 1996 |
Chemical modification of recombinant HIV-1 capsid protein p24 leads to the release of a hidden epitope prior to changes of the overall folding of the protein.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Antibodies, Monoclonal; Antibody Affinity; Circular Dichroism; Epitopes; Guanidine; Guanidines; HIV Antibodies; HIV Core Protein p24; Humans; Hydrogen-Ion Concentration; Lysine; Maleic Anhydrides; Molecular Sequence Data; Peptide Mapping; Protein Conformation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thermodynamics | 1996 |
Conformational changes of alpha-lactalbumin induced by the stepwise reduction of its disulfide bridges: the effect of the disulfide bridges on the structural stability of the protein in sodium dodecyl sulfate solution.
Topics: Anilino Naphthalenesulfonates; Animals; Cattle; Circular Dichroism; Detergents; Disulfides; Fluorescent Dyes; Guanidine; Guanidines; Lactalbumin; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Tryptophan | 1995 |
Preferential interaction of denaturants with rice bran lipase.
Topics: Enzyme Stability; Guanidine; Guanidines; Lipase; Oryza; Protein Denaturation; Thiocyanates; Urea | 1996 |
Characterization of an unfolding intermediate and kinetic analysis of guanidine hydrochloride-induced denaturation of the colicin E1 channel peptide.
Topics: Anilino Naphthalenesulfonates; Chromatography, Gel; Colicins; Cross-Linking Reagents; Dimerization; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Kinetics; Membrane Proteins; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 1997 |
Investigation of refolding condition for Pseudomonas fluorescens lipase by response surface methodology.
Topics: Arginine; Escherichia coli; Gene Expression; Glycerol; Guanidine; Guanidines; Hydrogen-Ion Concentration; Inclusion Bodies; Lipase; Protein Denaturation; Protein Folding; Pseudomonas fluorescens; Recombinant Proteins; Temperature | 1997 |
Activation of partially folded mitochondrial malate dehydrogenase by thioredoxin.
Topics: Anilino Naphthalenesulfonates; Animals; Chromatography, Gel; Circular Dichroism; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Malate Dehydrogenase; Mitochondria, Heart; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Swine; Thioredoxins | 1997 |
The influence of intersubunit cross-linking on the conformational changes of creatine kinase during denaturation by guanidine hydrochloride.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Creatine Kinase; Cross-Linking Reagents; Dimerization; Fluorescent Dyes; Guanidine; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Succinimides; Sulfhydryl Compounds | 1997 |
Complementation of peptide fragments of the single domain protein chymotrypsin inhibitor 2.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescent Dyes; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Peptides; Plant Proteins; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Serine Proteinase Inhibitors; Spectrometry, Fluorescence | 1997 |
Stability and solvent accessibility of SecA protein of Escherichia coli.
Topics: Adenosine Triphosphatases; Anilino Naphthalenesulfonates; Bacterial Proteins; Circular Dichroism; Enzyme Stability; Escherichia coli; Escherichia coli Proteins; Fluorescent Dyes; Guanidine; Hydrogen; Hydrolysis; Membrane Transport Proteins; Protein Denaturation; Protein Folding; SEC Translocation Channels; SecA Proteins; Solvents; Spectrometry, Fluorescence; Temperature; Tritium; Trypsin | 1997 |
Subdomain interactions as a determinant in the folding and stability of T4 lysozyme.
Topics: Anilino Naphthalenesulfonates; Bacteriophage T4; Circular Dichroism; Cloning, Molecular; Enzyme Stability; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Muramidase; Peptide Fragments; Protein Conformation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics | 1998 |
Cloning, overexpression, purification, and spectroscopic characterization of human S100P.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Base Sequence; Calcium; Calcium-Binding Proteins; Circular Dichroism; Cloning, Molecular; Escherichia coli; Fluorescent Dyes; Gene Expression; Guanidine; Humans; Molecular Sequence Data; Neoplasm Proteins; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Analysis, DNA; Spectrometry, Fluorescence | 1998 |
Binding of a burst-phase intermediate formed in the folding of denatured D-glyceraldehyde-3-phosphate dehydrogenase by chaperonin 60 and 8-anilino-1-naphthalenesulphonic acid.
Topics: Adenosine Triphosphate; Anilino Naphthalenesulfonates; Chaperonin 60; Dimerization; Enzyme Reactivators; Fluorescent Dyes; Glyceraldehyde-3-Phosphate Dehydrogenases; Guanidine; Protein Denaturation; Protein Folding | 1998 |
Oligomeric structure of hepatic lipase: evidence from a novel epitope tag technique.
Topics: Amino Acid Sequence; Animals; Cell Line; Centrifugation, Density Gradient; Dimerization; Epitopes; Genes, myc; Guanidine; Lipase; Liver; Molecular Sequence Data; Molecular Weight; Oligopeptides; Peptides; Protein Conformation; Protein Denaturation; Rats; Recombinant Proteins; Transfection | 1998 |
A monomeric mutant of Clostridium symbiosum glutamate dehydrogenase: comparison with a structured monomeric intermediate obtained during refolding.
Topics: Anilino Naphthalenesulfonates; Binding Sites; Circular Dichroism; Clostridium; Computer Simulation; Escherichia coli; Fluorescence; Glutamate Dehydrogenase; Guanidine; Molecular Weight; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Ultracentrifugation; Urea | 1998 |
Role of quaternary structure in muscle creatine kinase stability: tryptophan 210 is important for dimer cohesion.
Topics: Anilino Naphthalenesulfonates; Animals; Chromatography, High Pressure Liquid; Creatine Kinase; Dimerization; Enzyme Stability; Fluorescence; Guanidine; Hydroxymercuribenzoates; Isoenzymes; Mutagenesis, Site-Directed; Myocardium; Protein Conformation; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan | 1998 |
On the mechanism of human stefin B folding: II. Folding from GuHCl unfolded, TFE denatured, acid denatured, and acid intermediate states.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Cystatin B; Cystatins; Fluorescent Dyes; Guanidine; Humans; Hydrogen-Ion Concentration; Kinetics; Protein Denaturation; Protein Folding; Temperature | 1998 |
The effect of pH on the unfolding pathway and stability of ribosome-inactivating protein abrin-II.
Topics: Abrin; Anilino Naphthalenesulfonates; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence | 1998 |
Evidence for kinetic intermediate states during the refolding of GdnHCl-denatured MM-creatine kinase. Characterization of a trapped monomeric species.
Topics: Anilino Naphthalenesulfonates; Animals; Chemical Phenomena; Chemistry, Physical; Chromatography, Gel; Creatine Kinase; Cysteine; Enzyme Activation; Guanidine; Isoenzymes; Kinetics; Models, Chemical; Protein Binding; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Titrimetry; Tryptophan; Tyrosine | 1998 |
Unfolding and refolding of dimeric creatine kinase equilibrium and kinetic studies.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Creatine Kinase; Dimerization; Fluorescent Dyes; Guanidine; Kinetics; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Thermodynamics | 1998 |
The sequential mechanism of guanidine hydrochloride-induced denaturation of cAMP receptor protein from Escherichia coli. A fluorescent study using 8-anilino-1-naphthalenesulfonic acid.
Topics: Anilino Naphthalenesulfonates; Bacterial Proteins; Dimerization; Escherichia coli; Fluorescent Dyes; Guanidine; Kinetics; Protein Denaturation; Protein Folding; Receptors, Cyclic AMP; Spectrometry, Fluorescence | 1998 |
Opposite behavior of two isozymes when refolding in the presence of non-ionic detergents.
Topics: Anilino Naphthalenesulfonates; Animals; Aspartate Aminotransferases; beta-Cyclodextrins; Chaperonin 60; Cyclodextrins; Cytosol; Detergents; Dose-Response Relationship, Drug; Fluorescent Dyes; Guanidine; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Liver; Micelles; Mitochondria; Molecular Chaperones; Octoxynol; Polyethylene Glycols; Protein Folding; Rats; Spectrometry, Fluorescence; Temperature; Time Factors; Trypsin | 1998 |
The Mycobacterium tuberculosis small heat shock protein Hsp16.3 exposes hydrophobic surfaces at mild conditions: conformational flexibility and molecular chaperone activity.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescent Dyes; Guanidine; Heat-Shock Proteins; Insulin; Molecular Chaperones; Mycobacterium tuberculosis; Protein Conformation; Protein Denaturation | 1999 |
Guanidine hydrochloride-induced denaturation of Pseudomonas cepacia lipase.
Topics: Burkholderia cepacia; Calcium; Circular Dichroism; Dose-Response Relationship, Drug; Ethanol; Guanidine; Hydrogen-Ion Concentration; Ions; Kinetics; Lipase; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Thermodynamics; Time Factors | 1999 |
The molten globule state of a chimera of human alpha-lactalbumin and equine lysozyme.
Topics: Acrylamide; Amino Acid Sequence; Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Enzyme Stability; Fluorescent Dyes; Guanidine; Horses; Humans; Hydrogen-Ion Concentration; Lactalbumin; Molecular Sequence Data; Muramidase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Fusion Proteins; Solvents; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1999 |
Effect of self-association on the structural organization of partially folded proteins: inactivated actin.
Topics: Acrylamide; Actins; Anilino Naphthalenesulfonates; Animals; Guanidine; Protein Conformation; Protein Folding; Protein Multimerization; Protein Unfolding; Rabbits; Solvents; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan | 1999 |
The tetrameric protein transthyretin dissociates to a non-native monomer in solution. A novel model for amyloidogenesis.
Topics: Amyloid; Amyloid Neuropathies; Anilino Naphthalenesulfonates; Chromatography, Gel; Guanidine; Humans; Iodides; Models, Molecular; Prealbumin; Protein Conformation; Recombinant Proteins; Solubility; Spectrometry, Fluorescence; Tryptophan | 1999 |
Opposite effects of Ca(2+) and GTP binding on tissue transglutaminase tertiary structure.
Topics: Anilino Naphthalenesulfonates; Binding Sites; Calcium; Circular Dichroism; Enzyme Stability; Guanidine; Guanosine Triphosphate; Pressure; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Thermodynamics; Transglutaminases | 2000 |
Steady state and time resolved effects of guanidine hydrochloride on the structure of Humicola lanuginosa lipase revealed by fluorescence spectroscopy.
Topics: Amino Acid Substitution; Ascomycota; Binding Sites; Guanidine; Lipase; Mutation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2000 |
Fluorescence energy transfer indicates similar transient and equilibrium intermediates in staphylococcal nuclease folding.
Topics: Amino Acid Substitution; Anilino Naphthalenesulfonates; Anions; Circular Dichroism; Cysteine; Energy Transfer; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Models, Molecular; Pliability; Proline; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Thermodynamics; Titrimetry; Tryptophan; Urea | 2000 |
Tryptophan 140 is important, but serine 141 Is essential for the formation of the integrated conformation of staphylococcal nuclease.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescent Dyes; Guanidine; Micrococcal Nuclease; Peptide Fragments; Protein Folding; Recombinant Proteins; Serine; Spectrometry, Fluorescence; Tryptophan | 2000 |
Identification and characterization of an equilibrium intermediate in the unfolding pathway of an all beta-barrel protein.
Topics: Anilino Naphthalenesulfonates; Chromatography; Densitometry; Deuterium; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Fibroblast Growth Factor 1; Fibroblast Growth Factor 2; Fluorescent Dyes; Guanidine; Heparin; Humans; Hydrogen; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Time Factors; Urea | 2000 |
Characterization of acid-induced unfolding intermediates of glucose/xylose isomerase.
Topics: Aldose-Ketose Isomerases; Anilino Naphthalenesulfonates; Circular Dichroism; Dimerization; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Light; Osmolar Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Scattering, Radiation; Spectrophotometry, Ultraviolet; Streptomyces; Tryptophan | 2000 |
Matrix proteins from insect pliable cuticles: are they flexible and easily deformed?
Topics: Anilino Naphthalenesulfonates; Animals; Fluorescent Dyes; Grasshoppers; Guanidine; Hydrogen-Ion Concentration; Insect Proteins; Moths; Pliability; Protein Conformation; Protein Denaturation; Protein Folding | 2001 |
Cofactor-induced refolding: refolding of molten globule carbonic anhydrase induced by Zn(II) and Co(II).
Topics: Anilino Naphthalenesulfonates; Animals; Apoenzymes; Binding Sites; Carbonic Anhydrases; Cattle; Circular Dichroism; Cobalt; Fluorescent Dyes; Guanidine; Holoenzymes; Kinetics; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 2001 |
Characterization of the denatured states distribution of neocarzinostatin by small-angle neutron scattering and differential scanning calorimetry.
Topics: Anilino Naphthalenesulfonates; Antibiotics, Antineoplastic; Apoproteins; Calorimetry, Differential Scanning; Circular Dichroism; Fluorescent Dyes; Guanidine; Neutrons; Protein Denaturation; Protein Folding; Scattering, Radiation; Solutions; Spectrometry, Fluorescence; Zinostatin | 2001 |
Do human bile salt stimulated lipase and colipase-dependent pancreatic lipase share a common heparin-containing receptor?
Topics: Albumins; Binding Sites; Biosensing Techniques; Carrier Proteins; Colipases; Guanidine; Heparin; Humans; Lipase; Molecular Weight; Oligosaccharides; Osmolar Concentration; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Sequence Analysis, Protein; Static Electricity; Sterol Esterase; Sulfates | 2001 |
Capture of a dimeric intermediate during transthyretin amyloid formation.
Topics: Amyloid; Amyloid Neuropathies, Familial; Anilino Naphthalenesulfonates; Asparagine; Dimerization; Fluorescent Dyes; Glutamic Acid; Guanidine; Humans; Models, Molecular; Mutation; Prealbumin; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Staining and Labeling; Temperature; Thyroxine; Valine | 2001 |
Folding pathway for partially folded rabbit muscle creatine kinase.
Topics: Anilino Naphthalenesulfonates; Animals; Chaperonin 60; Circular Dichroism; Creatine Kinase; Dinitrobenzenes; Electrophoresis, Polyacrylamide Gel; Guanidine; Kinetics; Muscles; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet | 2001 |
Tryptophan mutants of intestinal fatty acid-binding protein: ultraviolet absorption and circular dichroism studies.
Topics: Anilino Naphthalenesulfonates; Carrier Proteins; Circular Dichroism; Dose-Response Relationship, Drug; Fatty Acid-Binding Proteins; Fluorescent Dyes; Guanidine; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Neoplasm Proteins; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Receptors, Retinoic Acid; Spectrophotometry; Spectrophotometry, Ultraviolet; Tryptophan; Ultraviolet Rays | 2001 |
Acid-induced partly folded conformation resembling a molten globule state of xylanase from an alkalothermophilic Bacillus sp.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bacillus; Bacterial Proteins; Circular Dichroism; Enzyme Stability; Fluorescence; Fluorescent Dyes; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Xylan Endo-1,3-beta-Xylosidase; Xylosidases | 2001 |
Multiple unfolding intermediates of human placental alkaline phosphatase in equilibrium urea denaturation.
Topics: Alkaline Phosphatase; Anilino Naphthalenesulfonates; Binding Sites; Circular Dichroism; Dose-Response Relationship, Drug; Enzyme Stability; Fluorescent Dyes; Guanidine; Humans; Kinetics; Models, Molecular; Placenta; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Salts; Sodium Chloride; Spectrometry, Fluorescence; Thermodynamics; Urea | 2001 |
Molecular mechanism for osmolyte protection of creatine kinase against guanidine denaturation.
Topics: Anilino Naphthalenesulfonates; Animals; Creatine Kinase; Fluorescent Dyes; Guanidine; Muscles; Osmolar Concentration; Protein Denaturation; Protein Structure, Secondary; Rabbits; Spectrometry, Fluorescence; Sucrose | 2001 |
Unique oligomeric intermediates of bovine liver catalase.
Topics: Anilino Naphthalenesulfonates; Animals; Catalase; Cattle; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Dimerization; Dose-Response Relationship, Drug; Fluorescent Dyes; Glutaral; Guanidine; Liver; Protein Folding; Protein Structure, Quaternary; Protein Structure, Tertiary; Sodium Chloride; Spectrometry, Fluorescence; Time Factors; Urea | 2002 |
Guanidinium chloride induced unfolding of a hemocyanin subunit from Carcinus aestuarii. I. Apo form.
Topics: Acrylamides; Anilino Naphthalenesulfonates; Animals; Apoproteins; Brachyura; Circular Dichroism; Fluorescence; Fluorescent Dyes; Guanidine; Hemocyanins; Protein Folding; Spectrophotometry, Ultraviolet; Thermodynamics | 2002 |
The folding of dimeric cytoplasmic malate dehydrogenase. Equilibrium and kinetic studies.
Topics: Anilino Naphthalenesulfonates; Chromatography, High Pressure Liquid; Circular Dichroism; Cytoplasm; Fluorescence; Guanidine; Kinetics; Light; Malate Dehydrogenase; Protein Denaturation; Protein Folding; Scattering, Radiation; Spectrophotometry, Ultraviolet; Time Factors | 2002 |
High-risk (HPV16) human papillomavirus E7 oncoprotein is highly stable and extended, with conformational transitions that could explain its multiple cellular binding partners.
Topics: Anilino Naphthalenesulfonates; Cell Transformation, Viral; Circular Dichroism; Dimerization; Electrophoresis, Polyacrylamide Gel; Guanidine; Hot Temperature; Humans; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Oncogene Proteins, Viral; Papillomaviridae; Papillomavirus E7 Proteins; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Risk Factors; Sodium Dodecyl Sulfate; Solvents | 2002 |
Acid and chemical induced conformational changes of ervatamin B. Presence of partially structured multiple intermediates.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Cysteine Endopeptidases; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectrophotometry; Urea | 2002 |
Evidence for the existence of an unfolding intermediate of thyroglobulin during denaturation by guanidine hydrochloride.
Topics: Anilino Naphthalenesulfonates; Animals; Cattle; Circular Dichroism; Guanidine; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Thyroglobulin | 2002 |
Generation of an active monomer of rabbit muscle creatine kinase by site-directed mutagenesis: the effect of quaternary structure on catalysis and stability.
Topics: Anilino Naphthalenesulfonates; Animals; Catalysis; Chromatography, Gel; Creatine Kinase; Dimerization; Enzyme Activation; Enzyme Stability; Fluorescent Dyes; Guanidine; Hot Temperature; Kinetics; Muscle, Skeletal; Mutagenesis, Site-Directed; Protein Denaturation; Protein Structure, Quaternary; Rabbits; Recombinant Proteins; Spectrometry, Fluorescence; Ultracentrifugation | 2003 |
Structure-to-function relationship of mini-lipoxygenase, a 60-kDa fragment of soybean lipoxygenase-1 with lower stability but higher enzymatic activity.
Topics: Anilino Naphthalenesulfonates; Animals; Arachidonic Acids; Binding Sites; Circular Dichroism; Dose-Response Relationship, Drug; Guanidine; Kinetics; Lipoxygenase; Models, Molecular; Protein Binding; Protein Conformation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Structure-Activity Relationship; Time Factors | 2003 |
Equilibrium studies of the effect of difference in sequence homology on the mechanism of denaturation of bovine and horse cytochromes-c.
Topics: Anilino Naphthalenesulfonates; Animals; Cattle; Circular Dichroism; Cytochrome c Group; Dose-Response Relationship, Drug; Fluorescent Dyes; Guanidine; Horses; Hot Temperature; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Sequence Alignment; Sequence Homology, Amino Acid; Spectrophotometry; Temperature | 2003 |
Detection of an intermediate during unfolding of bacterial cell division protein FtsZ: loss of functional properties precedes the global unfolding of FtsZ.
Topics: Anilino Naphthalenesulfonates; Bacterial Proteins; Circular Dichroism; Cytoskeletal Proteins; Dose-Response Relationship, Drug; Escherichia coli; Fluorescent Dyes; GTP Phosphohydrolases; Guanidine; Hydrolysis; Light; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Scattering, Radiation; Sodium Dodecyl Sulfate; Spectrophotometry; Surface-Active Agents; Temperature; Time Factors; Ultraviolet Rays; Urea | 2003 |
Stability studies on a lipase from Bacillus subtilis in guanidinium chloride.
Topics: Anilino Naphthalenesulfonates; Bacillus subtilis; Circular Dichroism; Enzyme Stability; Fluorescent Dyes; Guanidine; Lipase; Protein Binding; Protein Denaturation; Protein Folding | 2003 |
Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck delta2-crystallin.
Topics: Anilino Naphthalenesulfonates; Animals; Chromatography, Gel; Circular Dichroism; delta-Crystallins; Ducks; Fluorescent Dyes; Guanidine; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Ultracentrifugation | 2003 |
Induction of 'molten globule' like state in acid-denatured state of unmodified preparation of stem bromelain: implications of disulfides in protein folding.
Topics: Acids; Anilino Naphthalenesulfonates; Bromelains; Circular Dichroism; Disulfides; Fluorescence; Fluorescent Dyes; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Trifluoroethanol; Tryptophan | 2003 |
The unfolding intermediate state of calf intestinal alkaline phosphatase during denaturation in guanidine solutions.
Topics: Alkaline Phosphatase; Anilino Naphthalenesulfonates; Animals; Cattle; Guanidine; Intestines; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet | 2003 |
Low concentration of guanidine hydrochloride induces the formation of an aggregation-prone state in alpha-urease.
Topics: Anilino Naphthalenesulfonates; Canavalia; Circular Dichroism; Fluorescent Dyes; Guanidine; Light; Microscopy, Electron; Protein Conformation; Protein Denaturation; Scattering, Radiation; Urease | 2004 |
Refolding intermediate of guanidine hydrochloride denatured aminoacylase.
Topics: Amidohydrolases; Anilino Naphthalenesulfonates; Animals; Fluorescent Dyes; Guanidine; Protein Denaturation; Protein Folding; Spectrum Analysis; Zinc | 2004 |
Guanidinium chloride denaturation of the dimeric Bacillus licheniformis BlaI repressor highlights an independent domain unfolding pathway.
Topics: Anilino Naphthalenesulfonates; Bacillus; Bacterial Proteins; Centrifugation, Isopycnic; Chromatography, Gel; Circular Dichroism; Cross-Linking Reagents; DNA, Bacterial; Fluorescence; Gene Expression Regulation, Enzymologic; Guanidine; Molecular Probes; Nuclear Magnetic Resonance, Biomolecular; Peptides; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Repressor Proteins; Succinimides | 2004 |
Transient aggregation and stable dimerization induced by introducing an Alzheimer sequence into a water-soluble protein.
Topics: Alzheimer Disease; Amino Acid Sequence; Amyloid beta-Peptides; Anilino Naphthalenesulfonates; Dimerization; Guanidine; Humans; Hydrophobic and Hydrophilic Interactions; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Ribonucleoprotein, U1 Small Nuclear; RNA-Binding Proteins; Sequence Homology, Amino Acid; Solubility; Water | 2004 |
Compact molten globule-like state of hUBF HMG Box1 at extremely low pH.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescent Dyes; Guanidine; High Mobility Group Proteins; HMG-Box Domains; Humans; Hydrogen-Ion Concentration; Nuclear Magnetic Resonance, Biomolecular; Pol1 Transcription Initiation Complex Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Temperature | 2005 |
Unfolding and refolding of the glutamine-binding protein from Escherichia coli and its complex with glutamine induced by guanidine hydrochloride.
Topics: Anilino Naphthalenesulfonates; Carrier Proteins; Circular Dichroism; Escherichia coli; Escherichia coli Proteins; Fluorescence Polarization; Fluorescent Dyes; Glutamine; Guanidine; Macromolecular Substances; Models, Molecular; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tyrosine | 2005 |
Trehalose and 6-aminohexanoic acid stabilize and renature glucose-6-phosphate dehydrogenase inactivated by glycation and by guanidinium hydrochloride.
Topics: Aminocaproic Acid; Anilino Naphthalenesulfonates; Blotting, Western; Glucose; Glucosephosphate Dehydrogenase; Guanidine; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Trehalose | 2005 |
Partially folded intermediate state of concanavalin A retains its carbohydrate specificity.
Topics: Anilino Naphthalenesulfonates; Chromatography, Gel; Circular Dichroism; Concanavalin A; Guanidine; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet | 2005 |
Two distinct intermediates of trigger factor are populated during guanidine denaturation.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Cross-Linking Reagents; Dimerization; Electrophoresis, Polyacrylamide Gel; Escherichia coli Proteins; Guanidine; Molecular Chaperones; Muramidase; Peptidylprolyl Isomerase; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Succinimides; Thermodynamics | 2005 |
Specifically collapsed intermediate in the early stage of the folding of ribonuclease A.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescence; Guanidine; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Ribonuclease, Pancreatic; Thermodynamics; X-Ray Diffraction | 2005 |
A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea.
Topics: Anilino Naphthalenesulfonates; Bacterial Proteins; Cell Division; Cytoskeletal Proteins; Fluorescent Dyes; Glutamic Acid; GTP Phosphohydrolases; Guanidine; Methylamines; Parasympathomimetics; Protein Conformation; Protein Denaturation; Protein Folding; Urea | 2005 |
Effects of the stabilization of the molten globule state on the folding mechanism of alpha-lactalbumin: a study of a chimera of bovine and human alpha-lactalbumin.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Animals; Cattle; Fluorescent Dyes; Guanidine; Humans; Hydrophobic and Hydrophilic Interactions; Kinetics; Lactalbumin; Models, Molecular; Molecular Sequence Data; Peptides; Protein Folding; Protein Structure, Secondary; Recombinant Fusion Proteins; Spectrometry, Fluorescence | 2005 |
Time-resolved fluorescence studies on bovine serum albumin denaturation process.
Topics: Anilino Naphthalenesulfonates; Animals; Cattle; Fluorescent Dyes; Guanidine; Protein Denaturation; Serum Albumin, Bovine; Spectrometry, Fluorescence; Time Factors | 2006 |
Structure and stability of apolipoprotein a-I in solution and in discoidal high-density lipoprotein probed by double charge ablation and deletion mutation.
Topics: Anilino Naphthalenesulfonates; Animals; Apolipoprotein A-I; Baculoviridae; Circular Dichroism; Dose-Response Relationship, Drug; Gene Deletion; Guanidine; Humans; Lipids; Lipoproteins, HDL; Models, Biological; Mutation; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Solutions; Spectrometry, Fluorescence; Static Electricity; Temperature; Tryptophan; Tumor Cells, Cultured | 2006 |
Effect of carboxyl-terminal truncation on structure and lipid interaction of human apolipoprotein E4.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Apolipoprotein E4; Apolipoproteins E; Chromatography, Gel; Emulsions; Guanidine; Humans; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2006 |
Irreversible thermal denaturation of elongation factor Ts from Thermus thermophilus effect of the residual structure and intermonomer disulfide bond.
Topics: Algorithms; Anilino Naphthalenesulfonates; Calorimetry, Differential Scanning; Circular Dichroism; Cystine; Guanidine; Hot Temperature; Mutation; Peptide Elongation Factors; Protein Denaturation; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Thermus thermophilus | 2006 |
Effects of guanidine hydrochloride on the conformation and enzyme activity of streptomycin adenylyltransferase monitored by circular dichroism and fluorescence spectroscopy.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Circular Dichroism; Guanidine; Mycobacterium tuberculosis; Nucleotidyltransferases; Potassium Iodide; Protein Binding; Protein Conformation; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 2006 |
The partially unfolded state of beta-momorcharin characterized with steady-state and time-resolved fluorescence studies.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescence Polarization; Guanidine; Plant Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Ribosomal Proteins; Ribosome Inactivating Proteins; Spectrometry, Fluorescence | 2007 |
Identification and characterization of functional intermediates of stem bromelain during urea and guanidine hydrochloride unfolding.
Topics: Anilino Naphthalenesulfonates; Bromelains; Circular Dichroism; Ethanol; Fluorescence; Guanidine; Protein Conformation; Protein Denaturation; Protein Folding; Solvents; Structure-Activity Relationship; Urea | 2007 |
Kinetic intermediates of unfolding of dimeric prostatic phosphatase.
Topics: Acid Phosphatase; Anilino Naphthalenesulfonates; Congo Red; Dimerization; Dithionitrobenzoic Acid; Enzyme Inhibitors; Guanidine; Humans; In Vitro Techniques; Kinetics; Male; Prostate; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Tyrosine Phosphatases; Spectrometry, Fluorescence; Sulfhydryl Compounds | 2007 |
Characterization of the folding and unfolding reactions of single-chain monellin: evidence for multiple intermediates and competing pathways.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Computer Simulation; Glycine; Guanidine; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Phenylalanine; Plant Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Temperature | 2007 |
Tertiary structure in 7.9 M guanidinium chloride--the role of Glu53 and Asp287 in Pyrococcus furiosus endo-beta-1,3-glucanase.
Topics: Anilino Naphthalenesulfonates; Aspartic Acid; Binding Sites; Calcium; Chelating Agents; Dose-Response Relationship, Drug; Egtazic Acid; Enzyme Stability; Escherichia coli; Fluorescent Dyes; Glucan Endo-1,3-beta-D-Glucosidase; Glutamic Acid; Guanidine; Kinetics; Models, Biological; Models, Molecular; Mutation; Protein Binding; Protein Denaturation; Protein Renaturation; Protein Structure, Tertiary; Pyrococcus furiosus; Thermodynamics; Titrimetry | 2007 |
Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers.
Topics: Amines; Ascomycota; Colorimetry; Enzyme Activation; Enzyme Stability; Enzymes, Immobilized; Glyoxylates; Guanidine; Hot Temperature; Lipase; Polymers; Sepharose; Solvents; Time Factors; Titrimetry | 2009 |
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy.
Topics: Algorithms; Anilino Naphthalenesulfonates; Animals; Cattle; Guanidine; Membrane Proteins; Models, Chemical; Models, Molecular; Nonlinear Dynamics; Normal Distribution; Protein Denaturation; Protein Folding; Protein Stability; Regression Analysis; Serum Albumin, Bovine; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2010 |
Stabilization of folding intermediate States from alkaline induced unfolded state of bovine serum fetuin in trifluoroethanol and acetonitrile.
Topics: Acetonitriles; alpha-Fetoproteins; Anilino Naphthalenesulfonates; Animals; Blood Proteins; Cattle; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Microscopy, Fluorescence; Protein Folding; Protein Stability; Protein Structure, Secondary; Trifluoroethanol | 2010 |
Structure and activity of lysozyme on binding to ZnO nanoparticles.
Topics: Anilino Naphthalenesulfonates; Animals; Calorimetry; Catalytic Domain; Cross-Linking Reagents; Glutaral; Guanidine; Hydrogen-Ion Concentration; Models, Molecular; Muramidase; Nanoparticles; Particle Size; Protein Denaturation; Protein Multimerization; Protein Structure, Quaternary; Protein Structure, Secondary; Spectrometry, Fluorescence; Thermodynamics; Urea; Zinc Oxide | 2010 |
More stable structure of wheat germ lipase at low pH than its native state.
Topics: Acrylamide; Circular Dichroism; Enzyme Stability; Guanidine; Hydrogen-Ion Concentration; Lipase; Molecular Weight; Naphthalenesulfonates; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Subunits; Spectrometry, Fluorescence; Temperature; Triticum | 2010 |
Physicochemical properties of thiol proteinase inhibitor isolated from goat pancreas.
Topics: Anilino Naphthalenesulfonates; Animals; Chemical Phenomena; Circular Dichroism; Cystatins; Dimerization; Fluorescent Dyes; Goats; Guanidine; In Vitro Techniques; Pancreas; Protein Denaturation; Protein Structure, Secondary; Spectrometry, Fluorescence; Urea | 2010 |
The role of tryptophan in staphylococcal nuclease stability.
Topics: Anilino Naphthalenesulfonates; Bromosuccinimide; Circular Dichroism; Enzyme Stability; Guanidine; Micrococcal Nuclease; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Unfolding; Spectrometry, Fluorescence; Trifluoroethanol; Tryptophan | 2010 |
Differences in the pathways of proteins unfolding induced by urea and guanidine hydrochloride: molten globule state and aggregates.
Topics: Actins; Anilino Naphthalenesulfonates; Animals; Carbonic Anhydrase II; Cattle; Fluorescent Dyes; Guanidine; Hydrophobic and Hydrophilic Interactions; Kinetics; Muscle, Skeletal; Protein Conformation; Protein Denaturation; Protein Unfolding; Proteins; Rabbits; Spectrometry, Fluorescence; Urea | 2010 |
Protein unfolding studies of thiol-proteinase inhibitor from goat (Capra hircus) muscle in the presence of urea and GdnHCl as denaturants.
Topics: Anilino Naphthalenesulfonates; Animals; Cystatins; Goats; Guanidine; Muscles; Protease Inhibitors; Protein Denaturation; Protein Structure, Secondary; Protein Unfolding; Spectrometry, Fluorescence; Urea | 2011 |
In vitro refolding of triosephosphate isomerase from L. donovani.
Topics: Anilino Naphthalenesulfonates; Animals; Biotechnology; Blotting, Western; Cattle; Chromatography, Affinity; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genetic Vectors; Guanidine; Inclusion Bodies; Leishmania donovani; Protein Folding; Recombinant Fusion Proteins; Serum Albumin; Spectrometry, Fluorescence; Triose-Phosphate Isomerase | 2011 |
Folding dynamics of phenylalanine hydroxylase depends on the enzyme's metallation state: the native metal, iron, protects against aggregate intermediates.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescence; Guanidine; Iron; Metalloproteases; Metals; Molecular Dynamics Simulation; Phenylalanine Hydroxylase; Protein Conformation; Protein Denaturation; Protein Folding; Protein Unfolding; Thermodynamics; Ultracentrifugation | 2011 |
Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point.
Topics: Absorption; Acrylamide; Amyloid; Anilino Naphthalenesulfonates; Bromelains; Circular Dichroism; Enzyme Stability; Guanidine; Isoelectric Point; Nephelometry and Turbidimetry; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Trifluoroethanol; Tryptophan | 2011 |
Existence of different structural intermediates and aggregates on the folding pathway of ovalbumin.
Topics: Anilino Naphthalenesulfonates; Animals; Benzothiazoles; Dose-Response Relationship, Drug; Glucose; Glycation End Products, Advanced; Guanidine; Ovalbumin; Protein Denaturation; Protein Folding; Spectrum Analysis; Thiazoles; Time Factors | 2012 |
pH-Induced molten globule state of Rhizopus niveus lipase is more resistant against thermal and chemical denaturation than its native state.
Topics: Anilino Naphthalenesulfonates; Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Kinetics; Lipase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Rhizopus; Spectrometry, Fluorescence | 2012 |
Exploring the unfolding mechanism of γ-glutamyltranspeptidases: the case of the thermophilic enzyme from Geobacillus thermodenitrificans.
Topics: Amino Acid Substitution; Anilino Naphthalenesulfonates; Bacterial Proteins; Circular Dichroism; Enzyme Stability; Fluorescent Dyes; gamma-Glutamyltransferase; Geobacillus; Guanidine; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Mutagenesis, Site-Directed; Protein Structure, Secondary; Protein Unfolding; Spectrometry, Fluorescence; Transition Temperature | 2012 |
The folding pathway of glycosomal triosephosphate isomerase: structural insights into equilibrium intermediates.
Topics: Anilino Naphthalenesulfonates; Chromatography, Gel; Circular Dichroism; Cysteine; Enzyme Stability; Guanidine; Microbodies; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Folding; Protozoan Proteins; Thermodynamics; Triose-Phosphate Isomerase; Trypanosoma brucei brucei | 2012 |
Differing structural characteristics of molten globule intermediate of peanut lectin in urea and guanidine-HCl.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Guanidine; Peanut Agglutinin; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Protein Unfolding; Temperature; Tryptophan; Urea | 2012 |
Effect of signal peptide on stability and folding of Escherichia coli thioredoxin.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Buffers; Calorimetry, Differential Scanning; Chromatography, Gel; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Insulin; Molecular Sequence Data; Protein Denaturation; Protein Folding; Protein Refolding; Protein Sorting Signals; Protein Stability; Protein Structure, Quaternary; Proteolysis; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Temperature; Thioredoxins | 2013 |
A comparative study of unfolding of Erythrina cristagalli Lectin in chemical denaturant and Fluoroalcohols.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Erythrina; Guanidine; Hydrogen-Ion Concentration; Oxidation-Reduction; Plant Lectins; Propanols; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Trifluoroethanol; Tryptophan | 2014 |
Spectroscopic studies on unfolding processes of apo-neuroglobin induced by guanidine hydrochloride and urea.
Topics: Anilino Naphthalenesulfonates; Apoproteins; Circular Dichroism; Globins; Guanidine; Nerve Tissue Proteins; Neuroglobin; Protein Binding; Protein Unfolding; Spectrometry, Fluorescence; Thermodynamics; Urea | 2013 |
Mechanistic investigation of domain specific unfolding of human serum albumin and the effect of sucrose.
Topics: Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Guanidine; Hemin; Humans; Models, Molecular; Molecular Dynamics Simulation; Protein Denaturation; Protein Stability; Protein Structure, Tertiary; Protein Unfolding; Serum Albumin; Spectrometry, Fluorescence; Sucrose; Tryptophan; Water | 2013 |
Effect of chaotropes on lipase back extraction recovery in the process of reverse micellar extraction.
Topics: Animals; Cetrimonium; Cetrimonium Compounds; Guanidine; Hydrogen-Ion Concentration; Lipase; Liquid Phase Microextraction; Micelles; Pancreas; Solutions; Static Electricity; Surface-Active Agents; Swine; Urea; Water | 2014 |
Stability and activity of porcine lipase against temperature and chemical denaturants.
Topics: Animals; Enzyme Stability; Guanidine; Hot Temperature; Lipase; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Unfolding; Swine; Urea | 2014 |
A valine-to-lysine substitution at position 210 induces structural conversion of prion protein into a β-sheet rich oligomer.
Topics: Amino Acid Substitution; Amyloid; Anilino Naphthalenesulfonates; Cloning, Molecular; Escherichia coli; Fluorescent Dyes; Gene Expression; Guanidine; Hot Temperature; Humans; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Kinetics; Lysine; Models, Molecular; Mutation; Prion Proteins; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Recombinant Proteins; Thermodynamics; Valine | 2018 |
Effects of Single and Double Mutants in Human Glucose-6-Phosphate Dehydrogenase Variants Present in the Mexican Population: Biochemical and Structural Analysis.
Topics: Anilino Naphthalenesulfonates; Catalysis; Circular Dichroism; Glucosephosphate Dehydrogenase; Glucosephosphate Dehydrogenase Deficiency; Guanidine; Humans; Kinetics; Mexico; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Stability; Recombinant Proteins; Software; Temperature; Trypsin | 2020 |