goserelin and 16-hydroxyprogesterone

Is there any correlation between follicular fluid (FF) steroid levels and the occurrence of cytogenetic abnormalities in unfertilized human oocytes?. Cytogenetic analysis was carried out on 397 oocytes, and the steroid content of 104 corresponding FF was analyzed using high-pressure liquid chromatography. Ovarian stimulation was performed by clomiphene citrate and human menopausal gonadotropin (hMG) or by hMG combined with a gonadotropin-releasing hormone agonist (GnRH-a) pretreatment.. Oocyte maturity was correlated with an increasing FF progestin content and a significant decrease of androstenedione (A) levels. Chromosomal analysis revealed 84 of all oocytes to be abnormal (polyploid or aneuploid and/or prematurely condensed chromosomes present). In this group, A levels and A to estradiol ratios were significantly higher. Although progestin levels were higher in GnRH-a/hMG cycles, the incidence of oocyte normality was not different between the two stimulation schemes. More abnormal oocytes were found in patients with good sperm morphology.. Oocyte abnormality correlates with higher A levels in the corresponding FF. Oocyte fertilization is also determined by intrinsic oocytic factors other than maturity.

Topics: 17-alpha-Hydroxyprogesterone; 20-alpha-Dihydroprogesterone; Androstenedione; Buserelin; Chromosomes; Clomiphene; Estradiol; Female; Fertilization in Vitro; Follicular Fluid; Goserelin; Humans; Hydroxyprogesterones; Karyotyping; Male; Menotropins; Oocytes; Ploidies; Progesterone; Progestins; Steroids