glycoprotein-e2--hepatitis-c-virus and benzyl-salicylate

Starting point of the present paper was the result of a virtual screening using the open conformation of the large extracellular loop (LEL) of the CD81-receptor (crystal structure: PDB-ID: 1G8Q). After benzyl salicylate had been experimentally validated to be a moderate inhibitor of the CD81-LEL-HCV-E2 interaction, further optimization was performed and heterocyclic-substituted benzyl salicylate derivatives were synthesized. The compounds were tested for their ability to inhibit the interaction of a fluorescence-labeled antibody to CD81-LEL using HUH7.5 cells. No compound showed an increase concerning the inhibition of the protein-protein interaction compared to benzyl salicylate.

Topics: Antigens, CD; Antiviral Agents; Cell Line; Drug Evaluation, Preclinical; Humans; Protein Binding; Salicylates; Structure-Activity Relationship; Tetraspanin 28; Viral Envelope Proteins