glucoraphanin has been researched along with trioctylmethylammonium* in 1 studies
1 other study(ies) available for glucoraphanin and trioctylmethylammonium
| Article | Year |
|---|---|
Strong ion-exchange centrifugal partition chromatography as an efficient method for the large-scale purification of glucosinolates.
The glucosinolates sinalbin and glucoraphanin were purified by strong ion-exchange displacement centrifugal partition chromatography (SIXCPC). The optimized conditions involved the biphasic solvent system ethyl acetate/n-butanol/water (3:2:5, v/v), the lipophilic anion-exchanger Aliquat 336 (trioctylmethylammonium chloride, 160 and 408 mM) and a sodium iodide solution (80 and 272 mM) as displacer. Amounts as high as 2.4 g of sinalbin and 2.6g of glucoraphanin were obtained in one step in 2.5 and 3.5h respectively, starting from 12 and 25 g of mustard and broccoli seed aqueous extracts, using a laboratory scale CPC column (200 mL inner volume). Topics: Centrifugation; Chemical Fractionation; Choline; Chromatography, Ion Exchange; Glucosinolates; Hydrogen-Ion Concentration; Imidoesters; Molecular Structure; Mustard Plant; Oximes; Plant Extracts; Quaternary Ammonium Compounds; Seeds; Solvents; Sulfoxides; Water | 2007 |