glucoraphanin has been researched along with indole* in 3 studies
3 other study(ies) available for glucoraphanin and indole
| Article | Year |
|---|---|
Analysis of Processing Effects on Glucosinolate Profiles in Red Cabbage by LC-MS/MS in Multiple Reaction Monitoring Mode.
Red cabbage ( Topics: Brassica; Chromatography, Liquid; Cooking; Glucose; Glucosinolates; Imidoesters; Indoles; Microwaves; Oximes; Sulfoxides; Tandem Mass Spectrometry | 2021 |
Profiling glucosinolate metabolites in human urine and plasma after broccoli consumption using non-targeted and targeted metabolomic analyses.
Broccoli is a popular brassica vegetable and its consumption may decrease the occurrence of cancer in certain populations. To gain insight into the metabolites that may induce physiological responses to broccoli intake, a non-targeted metabolomic approach and a targeted approach for analysis of glucosinolate metabolites were developed using high resolution accurate mass spectrometry. A human study was conducted in which 6 subjects consumed a single meal of 200 g of uncooked broccoli florets. The metabolomic analysis revealed changes in endogenous metabolites and a decrease in hippuric acid after broccoli consumption. Targeted analysis using high-resolution, accurate mass-mass spectrometry (HRAM-MS) enabled detection of low concentrations (nM) of glucosinolate metabolites in human urine and plasma. Glucosinolate metabolites were found in human urine (13) and plasma (8), respectively. Metabolites from methoxyl-indole glucosinolates, arising from broccoli consumption, are reported for the first time. Most glucosinolate metabolites reached their peak concentration in urine 2-4 h after consumption while, in plasma, peak maxima were achieved 2 h after intake. The results suggest that glucoraphanin metabolites (sulforaphane, sulforaphane cysteine, sulforaphane N-acetyl cysteine) and indole metabolites (ascorbigen and methoxyl ascorbigen from indole glucosinolates) may serve as marker compounds for the intake of broccoli. Topics: Adult; Aged; Brassica; Female; Glucosinolates; Humans; Imidoesters; Indoles; Limit of Detection; Male; Mass Spectrometry; Metabolomics; Middle Aged; Oximes; Principal Component Analysis; Sulfoxides | 2020 |
Blanching impact on pigments, glucosinolates, and phenolics of dehydrated broccoli by-products.
Because of high water content, the valorisation of broccoli by-products requires dehydration that can preserve bioactive compounds. Blanching pre-treatment has been reported to improve the drying rate of broccoli. As a thermal treatment, it promotes also enzyme inactivation. Therefore, in this study, the impact of pre-dehydration blanching step, freeze-drying, air-drying at 40 °C, and microwave hydrodiffusion and gravity (MHG) dehydration on the levels of pigments, glucosinolates, and phenolics, was evaluated by UHPLC-DAD-ESI/MS Topics: Brassica; Chlorophyll; Desiccation; Food Ingredients; Glucosinolates; Imidoesters; Indoles; Microwaves; Oximes; Phenols; Pigments, Biological; Plant Extracts; Sulfoxides; Water | 2020 |