glucoiberin and sulforaphane

glucoiberin has been researched along with sulforaphane* in 4 studies

Trials

1 trial(s) available for glucoiberin and sulforaphane

ArticleYear
In vivo formation and bioavailability of isothiocyanates from glucosinolates in broccoli as affected by processing conditions.
    Molecular nutrition & food research, 2014, Volume: 58, Issue:7

    To study the effect of residual myrosinase (MYR) activity in differently processed broccoli on sulforaphane (SR) and iberin (IB) formation, bioavailability, and excretion in human volunteers.. Five different broccoli products were obtained with similar glucoraphanin (GR) and glucoiberin (GI) content, yet different MYR activity. Excretion of SR and IB conjugates in urine were determined in 15 participants after ingestion of the broccoli products. A reduction of 80% of MYR in the product did not cause differences in the total amount of SR and IB found in urine compared to the product with 100% MYR. Complete inactivation of MYR gave the lowest total amount of SR and IB in urine (10 and 19%). A residual MYR of only 2% in the product gave an intermediate amount (17 and 29%). The excretion half-lives of SR and IB conjugates were comparable for all the products (2.5 h on average), although the maximum excretion peak times were clearly shorter when the residual MYR was higher (2.3-6.1 h).. For the first time, the effect of residual MYR activity on isothiocyanate bioavailability was systematically and quantitatively studied. Processing conditions have a large effect on the kinetics and bioavailability of isothiocyanates from broccoli.

    Topics: Adult; Biological Availability; Brassica; Cross-Over Studies; Female; Glucosinolates; Glycoside Hydrolases; Healthy Volunteers; Humans; Imidoesters; Isothiocyanates; Male; Middle Aged; Oximes; Sulfoxides

2014

Other Studies

3 other study(ies) available for glucoiberin and sulforaphane

ArticleYear
Bioavailability of Isothiocyanates From Broccoli Sprouts in Protein, Lipid, and Fiber Gels.
    Molecular nutrition & food research, 2018, Volume: 62, Issue:18

    Optimization of bioavailability of dietary bioactive health-beneficial compounds is as important as increasing their concentration in foods. The aim of this study is to explore the change in bioavailability of isothiocyanates (ITCs) in broccoli sprouts incorporated in protein, fiber, and lipid gels.. Five participants took part in a cross-over study and collected timed urine samples up to 24 h after consumption of proteins, dietary fibers, and lipid gels containing broccoli sprouts powder. Sulforaphane and iberin metabolites were determined in the urine samples. Samples in which sulforaphane and iberin were preformed by myrosinase led to a higher bioavailability of those compounds. Compared to the control broccoli sprout, incorporation of sprouts in gels led to lower bioavailability for preformed sulforaphane and iberin (although for sulforaphane the lower bioavailability was not significantly different) whereas for the gels rich in their precursors, glucoraphanin and glucoiberin, the opposite trend was observed (although not significantly different).. This explorative study suggests that ITCs bioavailability can be modulated by food structure and composition and further and deeper investigations are needed to develop food products that lead to an optimized ITCs bioavailability.

    Topics: Adult; Body Mass Index; Brassica; Cross-Over Studies; Dietary Fats; Dietary Fiber; Dietary Proteins; Female; Gels; Glucosinolates; Glycoproteins; Humans; Imidoesters; Intracellular Signaling Peptides and Proteins; Isothiocyanates; Male; Middle Aged; Oximes; Plant Proteins; Powders; Sulfoxides; Young Adult

2018
The metabolism of methylsulfinylalkyl- and methylthioalkyl-glucosinolates by a selection of human gut bacteria.
    Molecular nutrition & food research, 2014, Volume: 58, Issue:4

    Certain myrosinase-positive human gut bacteria can metabolize glucosinolates (GSLs) to produce isothiocyanates (ITC) as chemopreventive agents. We investigated glucoerucin, glucoiberin, and glucoraphanin (present in broccoli) metabolism by human gut strains.. All tested bacteria metabolized glucoerucin to completion within 16 h to erucin and erucin nitrile (NIT). Lactobacillus agilis R16 metabolized only 10% of glucoiberin and glucoraphanin with no detectable products. Enterococcus casseliflavus CP1, however, metabolized 40-50% of glucoiberin and glucoraphanin producing relatively low concentrations of iberin and sulforaphane. Interestingly, Escherichia coli VL8 metabolized 80-90% of glucoiberin and glucoraphanin and also bioconverted glucoraphanin and glucoiberin to glucoerucin and glucoiberverin, respectively, producing erucin, erucin NIT, iberverin, and iberverin NIT from the two GSLs. The putative reductase enzyme in the cell-free extracts of this bacterium required both Mg(2+) and NAD(P)H as cofactors for bioconversion. The cell-free extract of E. coli VL8 containing the reductase enzyme was able to reduce both the GSL glucoraphanin and its hydrolysis product sulforaphane to glucoerucin and erucin/erucin NIT, respectively.. The composition and metabolic activity of the human gut bacteria can indirectly impact on the potential chemopreventive effects of GSL-derived metabolites.

    Topics: Brassica; Cell-Free System; Enterococcus; Escherichia coli; Gastrointestinal Tract; Glucose; Glucosinolates; Humans; Imidoesters; Isothiocyanates; Lactobacillus; Oximes; Sulfides; Sulfoxides; Thiocyanates

2014
Myrosinase hydrolysates of Brassica oleraceae L. var. italica reduce the risk of colon cancer.
    Phytotherapy research : PTR, 2012, Volume: 26, Issue:5

    By means of liquid chromatography-electrospray ionization (LC-ESI) mass spectrometry two glucosinolates, glucoiberin and 3-hydroxy,4(α-L-rhamnopyranosyloxy) benzyl glucosinolate, were identified in the aqueous extract of Brassica oleraceae L var. italica. Further, two compounds were isolated after enzymatic hydrolysis of the aqueous extract by myrosinase, one of them was identified as 4-vinyl-3-pyrazolidinone. The second compound (sulphoraphane) 1-isothiocyanate-4-methyl-sulphinyl butane, converted to the most stable form of thiourea (sulphoraphane thiourea). The crude extract (80% alcohol extract) of broccoli florets was examined for cytotoxic activity against different human cancer cell lines, it showed good inhibition of colon cancer (IC(50) 3.88 µg/mL). On the other hand each of the successive extracts (petroleum ether, chloroform, ethyl acetate and ethanol) showed no significant cytotoxic activity. When myrosinase hydrolysate was tested for cytotoxic activity on the colon cancer cell line it showed very high activity - 95% lethality up to 0.78 µg/mL.

    Topics: Antineoplastic Agents; Brassica; Cell Line, Tumor; Cell Survival; Chromatography, Liquid; Colonic Neoplasms; Flowers; Glucosinolates; Glycoside Hydrolases; Humans; Hydrolysis; Inhibitory Concentration 50; Isothiocyanates; Plant Extracts; Pyrazolones; Spectrometry, Mass, Electrospray Ionization; Sulfoxides; Thiourea

2012
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