glu-asp-arg and lysyl-glutamyl-aspartic-acid

This study was designed to estimate the neurobehavioral status and to compare the prevalence of psychoadaptive disorders among lorry-drivers (experimental group) and metal craftsmen (control group) in connection with their age, length of service, occupational hazards, work schedule and sociodemographic characteristics. 150 male lorry-drivers (mean age 43.3 +/- 0.9) and 150 male metal craftsmen (mean age 42.8 +/- 0.9) were examined using a clinical questionnaire to identify, estimate and compare neurotic states. The study comprised 3 groups: 1st--subjects with stable psychic adaptation, 2nd--subjects with unstable psychic adaptation, a risk group, 3rd--subjects with stable psychic disadaptation, i.e. with some borderline mental disorders (BMD). Significant differences in the prevalence of psychic adaptation and disadaptation among groups under study were found. The predominance of the 2nd and 3rd groups among lorry-drivers in comparison with control group was found. The results showed that social and demographic characteristics had no significant influence neither in experimental nor in control groups (p > 0.1). Variability of psychoemotional imbalance levels among lorry-drivers was found to be due to a combination of the following factors: occupational exposure and their work schedule, while in control group--to the age of metal craftsmen. Comparative analysis of neurobehavioral disorders revealed the predominance of the asthenic symptoms, anxious and depressive manifestations, hysterical reactions among lorry-drivers, and the vegetative disorders only in control group. The results thus obtained support the hypothesis of occupational hazards and long driving experience being the risk factors for the development of BMD. The application of bioregulating peptides was found to restore the organism adaptive potential, improved psychoemotional indices, intensified resistance to work stress and reduced occupational risk of borderline mental disorders (p < 0.001-0.05). The best effect was obtained in case of combined application of several cytogens (pinealon and vezugen), which were optimally selected regarding the effect of each adverse occupational factor on a target organ or system. The employed parameters of psychoemotional state were rather informative for assessing the peptidergic properties of cytogens in occupational medicine and geriatrics.

Topics: Adaptation, Psychological; Adult; Automobile Driving; Drug Therapy, Combination; Humans; Male; Mental Disorders; Motor Vehicles; Occupational Diseases; Oligopeptides; Treatment Outcome

In the monitoring process 110 representatives of different age groups held a comparative analysis of the efficacy and safety of several geroprotective techniques, including the use of dry carbon dioxide baths, hyperbaric oxygen therapy, therapeutic massage and receiving Oligopeptide preparations containing complexes lysyl-glutamyl-asparagin and glutamyl-asparagin-arginine (Vezugen and Pinealon). The most pronounced positive impact on indicators of biological age were detected during the combined use of these two Oligopeptide complexes. The most safe from the point of view of influence on a number of biochemical, immunological parameters, clinical condition of the patients were therapeutic massage and Oligopeptide drugs. The use of dry carbon dioxide baths and hyperbaric oxygenation with an undoubtedly positive influence on the indices of biological age has a number of limitations and contraindications regarding its security.. У 110 пациентов разного возраста проведен сравнительный анализ эффективности и безопасности нескольких геропротекторных методик, включавших использование сухих углекислых ванн, гипербарической оксигенации, лечебного массажа и приема олигопептидных препаратов, содержащих комплексы лизил-глутамил-аспарагин и глутамил-аспаригил-аргинин (Везуген и Пинеалон). Наиболее отчетливое позитивное влияние на показатели биологического возраста обнаружено при сочетанном применении двух названных олигопептидных комплексов. Наиболее безопасными с точки зрения воздействия на целый ряд биохимических, иммунных показателей и на клиническое состояние пациентов оказались лечебный массаж и олигопептидные препараты. Применение сухих углекислых ванн и гипербарической оксигенации, при несомненно позитивном влиянии на показатели биологического возраста, имеет ряд ограничений и противопоказаний.

Topics: Adult; Aged; Aging; Comparative Effectiveness Research; Female; Humans; Hyperbaric Oxygenation; Male; Massage; Middle Aged; Monitoring, Physiologic; Oligopeptides; Protective Agents; Russia; Treatment Outcome

We've estimated the cellular and metabolic part of geroprophylactic effects of short synthetic tripeptides vesugen and pinealon for correction of the biological age. 32 people (18 men, 12 women) aged 41-83 years with polymorbidity and the organic brain syndrome in remission participated in the study. The preparations of "Pinealon" and "Vesugen" have had the significant anabolic effect. They have improved the activity of the Central nervous system and other vital organs, which slows the rate of aging by biological age indicators. Vesugen has demonstrated more visible geroprophylactic effect than Pinealon. At the same time we've found the prooxidant activity through chemiluminescence. Decrease of markers CD34+ positive hematopoietic polypotent cells in blood has shown significant inhibition of hemopoiesis. Apparently, the cells have not been involved in the adaptive reactions. Pinealon and Vesugen haven't affected the degree of chromatin condensation, so they are safe on nuclear genetic level. This property should be studied in future. In geriatric practice, we recommend to apply the peptides Pinealon and Vesugen as geroprotectors anabolic neuroprotective and no antioxidant type for reducing the rate of aging in patients with the organic brain syndrome vascular and/or traumatic genesis.

Topics: Adult; Aged; Aged, 80 and over; Aging; Brain; Chronic Disease; Female; Humans; Male; Middle Aged; Neurocognitive Disorders; Oligopeptides; Treatment Outcome

Peptides Glu-Asp-Arg and Lys-Glu-Asp stimulate serotonin expression in aging cultures of brain cortex cells. Peptide regulation of 5-tryptophan hydroxylase gene encoding the enzyme involved in serotonin synthesis was demonstrated by the molecular docking method. The CCTGCC nucleotide sequence in 5-tryptophan hydroxylase gene was found to be complementary to these peptides. Hence, Glu-Asp-Arg and Lys-Glu-Asp peptides epigenetically regulate serotonin synthesis in the brain cortex, which indicates their neuro- and geroprotective activities.

Topics: Animals; Base Sequence; Binding Sites; Central Nervous System Stimulants; Cerebral Cortex; Gene Expression Regulation; Molecular Docking Simulation; Molecular Sequence Data; Oligopeptides; Primary Cell Culture; Protein Binding; Rats; Rats, Wistar; Serotonin; Serotonin Agents; Tryptophan Hydroxylase

We studied the effects of synthesized tripeptides T-32 (Glu-Asp-Ala), T-33 (Glu-Asp-Arg), T-34 (Glu-Asp-Gly), T-36 (Glu-Asp-Pro), and T-38 (Lys-Glu-Asp) on organotypic skin cultures of young and old rats. In skin explants from young rats, all peptides except T-34 produced a stimulating effect on cell proliferation. In skin explants from old rats, tripeptide T-38 produced a marked stimulatory effect on proliferation. Immunocytochemical study of the proapoptotic p53 protein expression showed that cell proliferation increased due to less pronounced apoptosis. The capacity of the studied tripeptides to promote cell proliferation in the skin tissues of young and old animals provides the basis for further study of these substances as preparations boosting the regenerative processes in the skin, including those at age-associated pathology.

Topics: Age Factors; Animals; Cell Proliferation; Cells, Cultured; Immunohistochemistry; Oligopeptides; Rats; Skin

We demonstrated the influence of short peptides on the expression of signaling molecules in organotypic culture of the pineal gland from 3-month-old rats. Peptides Ala-Glu-Asp-Gly and Lys-Glu-Asp stimulate the expression of proliferative protein Ki-67 in pineal gland culture. These peptides as well as Glu-Asp-Arg and Lys-Glu do not affect the expression of apoptosis marker AIF. The synthesis of transcription factor CGRP by pinealocytes was stimulated only by Ala-Glu-Asp-Gly. Thus, peptide Ala-Glu-Asp-Gly tissue-specifically stimulates proliferative and secretory activities of pinealocytes, which can be used for recovery of pineal gland functions at the molecular level.

Topics: Animals; Apoptosis Inducing Factor; Biomarkers; Calcitonin Gene-Related Peptide; Dipeptides; Intercellular Signaling Peptides and Proteins; Ki-67 Antigen; Oligopeptides; Pineal Gland; Rats; Rats, Wistar; Tissue Culture Techniques