germacrene-d and 1-1-diphenyl-2-picrylhydrazyl

The chemical composition of the essential oil of

Topics: Biphenyl Compounds; Free Radical Scavengers; Gas Chromatography-Mass Spectrometry; Inhibitory Concentration 50; Malaysia; Monocyclic Sesquiterpenes; Myristicaceae; Oils, Volatile; Picrates; Polycyclic Sesquiterpenes; Sesquiterpenes, Germacrane

Topics: Acyclic Monoterpenes; Antioxidants; Benzothiazoles; Biphenyl Compounds; Gas Chromatography-Mass Spectrometry; Monoterpenes; Oils, Volatile; Picrates; Plant Extracts; Polycyclic Sesquiterpenes; Scutellaria; Sesquiterpenes; Sesquiterpenes, Germacrane; Sulfonic Acids; Uzbekistan

The aims of this study were to investigate the chemical composition and the antioxidant activity and antibacterial activity of the essential oil of Xylopia sericea fruits (OXS). The fruits of this species are popularly used for medicinal purposes, and as a condiment in food preparation.. The chemical composition of OXS was analysed by GC/MS. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging, β-carotene/linoleic acid bleaching and phosphomolybdenum and thiobarbituric acid-reactive substance (TBARS) assays were used to evaluate the antioxidant activity. Antibacterial activity was assessed by minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against bacterial strains of interest to human health and food spoilage.. Eighty-four compounds were identified. The sesquiterpenes spathulenol (16.42%), guaiol (13.93%) and germacrene D (8.11%) were the most abundant constituents. OXS presented a significant antioxidant activity and also a high bacteriostatic effect against Staphylococcus aureus, Enterobacter cloacae, Bacillus cereus and Klebsiella pneumoniae.. Those results evidenced the potential of OXS to treat human bacterial infections and as an antimicrobial ingredient for food preservation.

Topics: Anti-Bacterial Agents; Antioxidants; Bacteria; Biphenyl Compounds; Brazil; Fruit; Microbial Sensitivity Tests; Oils, Volatile; Picrates; Plant Extracts; Plant Oils; Sesquiterpenes; Sesquiterpenes, Germacrane; Sesquiterpenes, Guaiane; Xylopia

This study aimed to evaluate the variations of the chemical composition and bioactivity of essential oils of Liquidambar styraciflua L. (Altingiaceae) collected in different seasons.. The oils were analysed by GLC/FID and GLC/MS. The antioxidant activity was investigated by diphenylpicrylhydrazyl (DPPH) and superoxide anion radical scavenging assays and the deoxyribose degradation assay. Inhibition of both 5-lipoxygenase (5-LOX) and prostaglandin E2 (PGE2) production in hepatic cancer (HepG-2) cells were used to assess the anti-inflammatory activity. The cytotoxic activity was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.. Altogether, 64 volatile secondary metabolites were identified. The major components of the leaf oil were d-limonene, α-pinene and β-pinene, and of the stem oil were germacrine D, α-cadinol, d-limonene, α-pinene, and β-pinene. Leaf and stem oils collected in spring could reduce DPPH● (IC50 = 3.17 and 2.19 mg/ml) and prevent the degradation of the deoxyribose sugar (IC50 = 17.55 and 14.29 μg/ml). The stem oil exhibited a higher inhibition of both 5-LOX and PGE2 than the leaf oil. The cytotoxic activity of leaf and stem oils was low in cancer cell lines (IC50 = 136.27 and 119.78 μg/ml in cervical cancer (HeLa) cells).. Essential oils of L. styraciflua exhibited an interesting anti-inflammatory activity with low cytotoxicity, supporting its traditional use to treat inflammation.

Topics: Anti-Inflammatory Agents; Antioxidants; Arachidonate 5-Lipoxygenase; Bicyclic Monoterpenes; Biphenyl Compounds; Bridged Bicyclo Compounds; Cyclohexenes; Deoxyribose; Dinoprostone; HeLa Cells; Hep G2 Cells; Humans; Limonene; Liquidambar; Monoterpenes; Neoplasms; Oils, Volatile; Picrates; Plant Extracts; Plant Leaves; Plant Stems; Sesquiterpenes, Germacrane; Superoxides; Terpenes