germacrene-a has been researched along with beta-elemene* in 3 studies
*beta-elemene: increases tumor cell immunogenicity by inducing, at least in part, elevated expression of heat shock protein 70 on tumor cell surface [MeSH]
*beta-elemene: increases tumor cell immunogenicity by inducing, at least in part, elevated expression of heat shock protein 70 on tumor cell surface [MeSH]
3 other study(ies) available for germacrene-a and beta-elemene
| Article | Year |
|---|---|
Engineering Escherichia coli BL21 (DE3) for high-yield production of germacrene A, a precursor of β-elemene via combinatorial metabolic engineering strategies.
Topics: Acetyl Coenzyme A; Carbon; Escherichia coli; Metabolic Engineering; Sesquiterpenes | 2023 |
Combination of microbial and chemical synthesis for the sustainable production of β-elemene, a promising plant-extracted anticancer compound.
Beta-elemene, a class of sesquiterpene derived from the Chinese medicinal herb Curcuma wenyujin, is widely used in clinical medicine due to its broad-spectrum antitumor activity. However, the unsustainable plant extraction prompted the search for environmentally friendly strategies for β-elemene production. In this study, we designed a Yarrowia lipolytica cell factory that can continuously produce germacrene A, which is further converted into β-elemene with 100% yield through a Cope rearrangement reaction by shifting the temperature to 250°C. First, the productivity of four plant-derived germacrene A synthases was evaluated. After that, the metabolic flux of the precursor to germacrene A was maximized by optimizing the endogenous mevalonate pathway, inhibiting the competing squalene pathway, and expressing germacrene A synthase gene in multiple copies. Finally, the most promising strain achieved the highest β-elemene titer reported to date with 5.08 g/L. This sustainable and green method has the potential for industrial β-elemene production. Topics: Metabolic Engineering; Plant Extracts; Sesquiterpenes; Sesquiterpenes, Germacrane; Yarrowia | 2023 |
Metabolic engineering of Saccharomyces cerevisiae for production of germacrene A, a precursor of beta-elemene.
Beta-elemene, a sesquiterpene and the major component of the medicinal herb Curcuma wenyujin, has antitumor activity against various types of cancer and could potentially serve as a potent antineoplastic drug. However, its current mode of production through extraction from plants has been inefficient and suffers from limited natural resources. Here, we engineered a yeast cell factory for the sustainable production of germacrene A, which can be transformed to beta-elemene by a one-step chemical reaction in vitro. Two heterologous germacrene A synthases (GASs) converting farnesyl pyrophosphate (FPP) to germacrene A were evaluated in yeast for their ability to produce germacrene A. Thereafter, several metabolic engineering strategies were used to improve the production level. Overexpression of truncated 3-hydroxyl-3-methylglutaryl-CoA reductase and fusion of FPP synthase with GAS, led to a sixfold increase in germacrene A production in shake-flask culture. Finally, 190.7 mg/l of germacrene A was achieved. The results reported in this study represent the highest titer of germacrene A reported to date. These results provide a basis for creating an efficient route for further industrial application re-placing the traditional extraction of beta-elemene from plant sources. Topics: Batch Cell Culture Techniques; Culture Media; Gene Expression Regulation, Fungal; Geranyltranstransferase; HMGB1 Protein; Metabolic Engineering; Plasmids; Polyisoprenyl Phosphates; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sesquiterpenes; Sesquiterpenes, Germacrane | 2017 |