genipin-1-gentiobioside and geniposide

The traditional Chinese medicine formula Fuling Decoction (FD) has been clinically used for eczema treatment, but the unclear chemical distribution and the lack of quality control have strongly restricted its application. In this study, an analytical method incorporating ultra-fast liquid chromatography (UFLC) with MS and UV detection was developed for rapid profiling of the chemical constitutes from FD. Fourteen constitutes were identified by UFLC-ESI-MS, while four major components including genipingentiobioside, geniposide, paeoniflorin and liquiritin were quantified simultaneously by UFLC-DAD. The UFLC-based method was fully validated and can be applied to screening and determination of principal components in commercially FD prescriptions.

Topics: Benzoates; Bridged-Ring Compounds; Chromatography, Liquid; Drugs, Chinese Herbal; Flavanones; Glucosides; Iridoids; Monoterpenes; Quality Control; Spectrometry, Mass, Electrospray Ionization

A new iridoid, gardaloside (1), and a new safranal-type monoterpene, jasminoside G (2), together with 10 known compounds including nine iridoids and a second safranal-type monoterpene, were isolated from the fruits of Gardenia jasminoides. The structures of 1 and 2 were established on the basis of spectroscopic evidence. Of these compounds, geniposide (3), 6alpha-hydroxygeniposide (5), ixoroside (7), and shanzhiside (8) showed significant inhibition of IL-2 secretion by phorbol myristate acetate and anti-CD28 monoclonal antibody co-stimulated activation of human peripheral blood T cells.

Topics: Antibodies, Monoclonal; CD28 Antigens; Fruit; Gardenia; Humans; Immunosuppressive Agents; Interleukin-2; Iridoids; Molecular Structure; Monoterpenes; Plants, Medicinal; Pyrans; T-Lymphocytes; Taiwan; Tetradecanoylphorbol Acetate

Topics: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Glucosides; Glycosides; Iridoid Glucosides; Iridoids; Pyrans