gemifloxacin and 2--deoxyguanosine-5--phosphate

The photochemical properties of gemifloxacin (GEFX), a fluoroquinolone antibacterial drug that exhibits phototoxicity toward biological substrates, were studied in aqueous solutions by laser flash photolysis (LFP) and pulse radiolysis. GEFX triplet state ((3)GEFX(∗)) absorption spectra showed maximum absorption at 510nm. (3)GEFX(∗) was quenched by naproxen (NAP) via energy transfer with a rate constant of 1.2×10(8)dm(3)mol(-1)s(-1). The energy of (3)GEFX(∗) was 266kJmol(-1) and the transient absorption spectra showed direct evidence of electron transfer from 2'-deoxyguanosine-5'-monophosphate, N,N,N',N'-tetramethyl-p-phenylenediamine, and tryptophan to (3)GEFX(∗) with bimolecular reaction rate constants of 4.1×10(6), 2.0×10(7), and 2.2×10(7)dm(3)mol(-1)s(-1), respectively. The rate constants for reactions of GEFX with OH, eaq(-) were found to be 1.5×10(10) and 1.4×10(10)dm(3)mol(-1)s(-1), respectively. The mechanisms and products of the photosensitive damage of lysozyme were related to the GEFX concentration, irradiation time, and ambient conditions.

Topics: Deoxyguanine Nucleotides; Electron Transport; Fluoroquinolones; Gemifloxacin; Hydroxyl Radical; Kinetics; Lasers; Naphthyridines; Photolysis; Tetramethylphenylenediamine; Tryptophan