gastrins and deoxyinosine

gastrins has been researched along with deoxyinosine* in 2 studies

Other Studies

2 other study(ies) available for gastrins and deoxyinosine

Article	Year
An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions. The Journal of biological chemistry, 1985, Mar-10, Volume: 260, Issue:5 Two deoxyoligonucleotide probes (23-mer and 26-mer) carrying deoxyinosine residues (I) at positions corresponding to ambiguous nucleotides derived from amino acid sequence have been synthesized by the phosphotriester method using a polymer support. The 23-mer and 26-mer corresponded to the mRNA for 8 amino acids from gastrin and 9 amino acids from cholecystokinin, respectively. The dIs have been used where the base in the third position of the amino acid codon is ambiguous. These deoxyoligonucleotides were used as probes for hybridization with colonies containing the corresponding cDNAs or genes. The hybrid formed between a gastrin clone and the 23-mer that harbors 5 dIs was dissociated at 50-55 degrees C, suggesting that deoxyinosine did not significantly effect the stabilization or destabilization of the DNA duplex. A similar result was obtained using the 26-mer that contains 5 dIs and a phage clone DNA of the cholecystokinin gene. Thus oligonucleotide probes with deoxyinosine residues at ambiguous points seem to be useful as hybridization probes for cloning genes for proteins containing amino acids with degenerate codons. Topics: Base Sequence; Codon; Deoxyribonucleotides; DNA; Gastrins; Inosine; Nucleic Acid Hybridization; RNA, Messenger; Temperature; Templates, Genetic	1985
Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine. Proceedings of the National Academy of Sciences of the United States of America, 1985, Volume: 82, Issue:7 A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of the isolated gene revealed that human preprocholecystokinin consists of 115 amino acid residues, with 11 amino acids in common with the human gastrin precursor, another member of the gastrin-CCK family, and that the coding region is separated by a single, long intron. CCK appears to be encoded by a single-copy gene in the haploid human genome, as revealed by genomic Southern hybridization analysis, suggesting that the same gene is expressed both in gut and brain. Topics: Amino Acid Sequence; Base Sequence; Cholecystokinin; Cloning, Molecular; Gastrins; Genes, Synthetic; Humans; Inosine; Nucleic Acid Hybridization	1985

Article

Year

An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.

The Journal of biological chemistry, 1985, Mar-10, Volume: 260, Issue:5

Two deoxyoligonucleotide probes (23-mer and 26-mer) carrying deoxyinosine residues (I) at positions corresponding to ambiguous nucleotides derived from amino acid sequence have been synthesized by the phosphotriester method using a polymer support. The 23-mer and 26-mer corresponded to the mRNA for 8 amino acids from gastrin and 9 amino acids from cholecystokinin, respectively. The dIs have been used where the base in the third position of the amino acid codon is ambiguous. These deoxyoligonucleotides were used as probes for hybridization with colonies containing the corresponding cDNAs or genes. The hybrid formed between a gastrin clone and the 23-mer that harbors 5 dIs was dissociated at 50-55 degrees C, suggesting that deoxyinosine did not significantly effect the stabilization or destabilization of the DNA duplex. A similar result was obtained using the 26-mer that contains 5 dIs and a phage clone DNA of the cholecystokinin gene. Thus oligonucleotide probes with deoxyinosine residues at ambiguous points seem to be useful as hybridization probes for cloning genes for proteins containing amino acids with degenerate codons.

Topics: Base Sequence; Codon; Deoxyribonucleotides; DNA; Gastrins; Inosine; Nucleic Acid Hybridization; RNA, Messenger; Temperature; Templates, Genetic

1985

Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine.

Proceedings of the National Academy of Sciences of the United States of America, 1985, Volume: 82, Issue:7

A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of the isolated gene revealed that human preprocholecystokinin consists of 115 amino acid residues, with 11 amino acids in common with the human gastrin precursor, another member of the gastrin-CCK family, and that the coding region is separated by a single, long intron. CCK appears to be encoded by a single-copy gene in the haploid human genome, as revealed by genomic Southern hybridization analysis, suggesting that the same gene is expressed both in gut and brain.

Topics: Amino Acid Sequence; Base Sequence; Cholecystokinin; Cloning, Molecular; Gastrins; Genes, Synthetic; Humans; Inosine; Nucleic Acid Hybridization

1985