gamma-sitosterol and protocatechuic-acid

To study the chemical constituents of stalk form Pottsia laxifora.. The chemical constituents were isolated by various chromatography methods,and the structures were elucidated by spectroscopic methods,physical and chemical properties.. Twelve compounds were isolated and their structures were identified as β-sitosterol( 1),salicylic acid( 2),resorcinol( 3),4-hydroxybenzoic acid( 4),protocatechuic acid( 5),vanillic acid( 6),syringic acid( 7),scopoletin( 8),cleomiscosin D( 9),( +)-lyoniresinol( 10),(-)-loliolide( 11) and daucosterol( 12).. Compounds 1 ~ 11 are isolated from this plant for the first time.

Topics: Coumarins; Gallic Acid; Hydroxybenzoates; Magnoliaceae; Scopoletin; Sitosterols; Vanillic Acid

Landolphia owariensis P. Beauv is economically important for latex/rubber and folklore medicine. Its stringy seed pulp is freely eaten by humans and animals. Thus, L. owariensis stringy seed pulp was extracted serially with hexane and acetone to isolate and characterize its active pharmaceutical ingredients. Solvent/solvent partition and chromatographic separations afforded four bioactive compounds, (E)-3-(3,4-Dihydroxylcinnamoyl)quinic acid [(E)-Chlorogenic acid], I; (E)-3-(3,4-Dihydroxylcinnamoyl)quinic acid methyl ester [(E)-Chlorogenic acid methyl ester], II; 3,4-Dihydroxylbenzoic acid, (Protocatechuic acid), III; and 22,23-Dihydrostigmaster-3β-ol (3β-Sitosterol) (IV). Structures of I, II and III were assigned by combinations of high-performance liquid chromatography-ultraviolet-visible spectroscopy, 1D and 2D nuclear magnetic resonance spectroscopy, high-performance liquid chromatography-mass spectrometry and reference to published literatures, while compound IV was identified by chemical methods and gas chromatography-mass spectrometry. The phenylpropanoids and phenolic acid (compounds I, II and III) are notable standard antioxidants with confirmed hepatic-protective activity and other exciting biological activities. Compound IV has been reported to possess anti-inflammatory activity, anti-colon cancer action and a cholesterol-lowering effect. The described compounds are important medicinal constituents of L. owariensis stringy seed pulp, and this is the first major report on the phytochemistry of L. owariensis P. Beauv.

Topics: Apocynaceae; Chlorogenic Acid; Chromatography, High Pressure Liquid; Gas Chromatography-Mass Spectrometry; Hydroxybenzoates; Phytosterols; Plant Extracts; Quinic Acid; Seeds; Sitosterols

To study the chemical constituents from the seeds of Euryale ferox.. The chemical constituents were isolated by silica gel column, Sephadex LH-20 and their structures were identified by physico-chemical and spectral analysis.. Seven compounds were purified from the 95% ethanol extract. These constituents were elucidated as protocatechuic acid (1), gallic acid (2), gallic acid ethyl ester(3),5 ,7-dihydroxychromone(4), β-sitosterol(5), daucosterol(6), and 5,7-dihydroxy-6,4'-dimethoxyflavone(7), respectively.. All compounds are isolated from this plant for the first time.

Topics: Gallic Acid; Hydroxybenzoates; Nymphaeaceae; Phytochemicals; Seeds; Sitosterols

To investigate the chemical constituents in the seaweed Sargassum naozhouense collected from the sea around Zhanjiang.. The compounds were isolated and purified by various chromatographic techniques including silica gel chromatography, Sephadex LH-20 gel chromatography as well as preparative thin layer chromatography, and their structures were identified by spectral analysis.. Ten compounds were isolated and identified as 1-O-hexadecanoyl glycerol (1), pheophytin a(2), β-sitosterol (3), mannitol (4), uracil (5), thymine(6),p-hydroxybenzoic acid(7) 3,4-dihydroxybenzoic acid (8) 4-hydroxyphthalide (9), and 2'-de-cxythymidine( 10).. All the compounds are isolated from the seaweed Sargassum naozhouense for the first time.

Topics: Chromatography, Gel; Hydroxybenzoates; Pheophytins; Sargassum; Seaweed; Sitosterols

This work reports isolation of an unusual lignan with a bicyclic [2.2.2] octene skeleton, named rufescenolide (1), from stems of Cordia rufescens, along with β-sitosterol, stigmasterol, syringaldehyde, 3-β-O-D-glucopyranosyl-sitosterol, methyl caffeate, 4-methoxy-protocatechuic acid and methyl rosmarinate. Structural characterizations employed IR spectroscopic, ESIHRMS and mono and dimensional NMR spectroscopy.

Topics: Bridged Bicyclo Compounds; Caffeic Acids; Cordia; Hydroxybenzoates; Lignans; Magnetic Resonance Spectroscopy; Molecular Structure; Octanes; Plant Stems; Sitosterols; Stigmasterol

To study the chemical constituents of Asarum himalaicum, fifteen compounds were isolated from a 70% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, and semi-preparative HPLC. By spectroscopic techniques including 1H NMR, 13C NMR, and HR-ESI-MS, these compounds were identified as 4-demethoxyaristolochic acid BII (1), aristolochic acid I (2), aristolochic acid Ia (3), 7-hydroxyaristolochic acid I (4), aristolochic acid IV (5), aristolic acid II (6), debilic acid (7), aristololactam I (8), 9-hydroxyaristololactam I (9), 7-methoxyaristololactam IV (10), (2S)-narigenin-5, 7-di-O-beta-D-pyranosylglucoside (11), 4-hydroxybenzoic acid (12), 3, 4-dihydroxybenzoic acid (13), 4-hydroxycinnamic acid (14), and beta-sitosterol (15). All of these compounds (1-15) were obtained from A. himalaicum for the first time. Among them, 1 was identified as a new compound, and compounds 3-6, 9, 12-14 were isolated from Asarum genus for the first time. Since the kidney toxicity of aristolochic acids and aristololactams has been reported, the result of this investigation suggests that it should be cautioned to use A. himalaicum as a medicine.

Topics: Aristolochic Acids; Asarum; Chromatography, High Pressure Liquid; Coumaric Acids; Hydroxybenzoates; Magnetic Resonance Spectroscopy; Molecular Structure; Parabens; Plants, Medicinal; Propionates; Sitosterols; Spectrometry, Mass, Electrospray Ionization

To study the chemical constituents in the fruits of Acanthopanax gracilistylus.. The chemical components were isolated and purified by silica gel, ODS C-18, and Sephadex LH-20 column chromatogram. The chemical structures were elucidated on the basis of physicochemical properties and spectral data.. Ten compounds were isolated and identified as acankoreoside D(1), 3alpha, 11alpha-dihydroxylup-20(29)-en-28-oic acid(2), 3/3-([O-beta-D-glucopyranuronosyl] oxy) -olean-12-ene-28-olc acid (3),3beta-([O-beta-D-glucopyranuronosyl]oxy)-28-O-P3-D-glucopyranosyl-olean-12-ene-28-olc acid(4),oleanolic acid-3-O-6'-O-methyl-beta-D-glucuronopyranoside(5), acantrifoside A(6), acankoreoside A(7), (-)-kaur-16-en-19-oic acid(8), protocatechuic acid (9),beta-sitosterol(10).. Compounds 2-5 are obtained from the fruits of the plant for the first time.

Topics: Eleutherococcus; Ethanol; Fruit; Hydroxybenzoates; Magnetic Resonance Spectroscopy; Plants, Medicinal; Saponins; Sitosterols; Triterpenes

To study the chemical constituent from the antithrombus activity extract of Radix Aerio Fici Microcarpae.. The isolation and purification of the extract were conducted by means of silica gel column chromatography, polyamide column chromatography and recrytallization. The structures of these compounds were elucidated based on the physicochemical properties and spectroscopic analysis.. 7 compounds were isolated from the extract. They were identified as isovanillic acid (1), 4-hydroxybenzoic acid(2),3,4-dihydroxybenzoic acid (3), friedelane (4), alpha-amyrin acetate (5), lanosterol (6) and beta-sitosterol (7) .. Compound 3,4 and 7 are isolated from this plant for the first time.

Topics: Fibrinolytic Agents; Ficus; Hydroxybenzoates; Lanosterol; Molecular Structure; Oleanolic Acid; Parabens; Plant Roots; Sitosterols; Triterpenes; Vanillic Acid

To study the chemical constituents of Geranium eristemon.. Chromatography and spectral analysis were used to isolate the constituents and elucidate their structures.. Five compounds were isolated from acetone extract of the whole grass of G. eristemon, and identified as beta-sitosterol, protocatechuic acid, myricetin, kaempferol-7-O-alpha-L-arabifuranoside and kaempferol-3-O-alpha-L-arabifuranoside.. kaempferol-7-O-alpha-L-arabifuranoside and kaempferol-3-O-alpha-L-arabifuranoside were isolated from G. genus for the first time.

Topics: Arabinose; Flavonoids; Geranium; Hydroxybenzoates; Kaempferols; Plants, Medicinal; Sitosterols

Seven compounds were isolated from Neonauclea sessilifolia. They are paeonol, 3,4-dihydroxybenzoic acid, scopoletin, anthraquinnones chrysophanol, 4H-1-benzopyran-4-one,5,7-dihydroxy-2-methyl, beta-sitosterol and stigmasterol glucoside. These compounds were isolated at first time from this genus and this plant.

Topics: Acetophenones; Gas Chromatography-Mass Spectrometry; Glucosides; Hydroxybenzoates; Plant Stems; Plants, Medicinal; Rubiaceae; Scopoletin; Sitosterols; Stigmasterol

Seven compounds were isolated from Alinia officinarum Hance and were identified as beta-sitoterol, 1,7-diphenyl-5-ol-3-heptone, 1-phenyl-7-(3'-methoxyl-4'-hydroxyl) phenyl-5-ol-3-heptone, glandin, kaempferol-4'-methylether and 3,4-dihydroxylbenzoic acid by IR, 1HNMR, 13CNMR, FAB-MS and EA. Among these compounds, 3,4-dihydroxylbenzoic acid was the first time obtained from Alpinia officinarum Hance. Furthermore, 1-phenyl-7-(3'-methoxyl-4'-hydroxyl) phenyl-5-ol-3-heptone and a new compound 1,7-diphenyl-3,5-heptandiol-phenyl-7-(3'-methoxyl-4'-hydroxyl) phenyl-3,5-heptaxdiol were obtained from 1,7-diphenyl-5-ol-3-heptone and 1-phenyl-7-(3'-methoxyl-4'-hydroxyl) phenyl-5-ol-3-heptone via chemical reductions.

Topics: Alpinia; Flavonoids; Hydroxybenzoates; Kaempferols; Plant Roots; Plants, Medicinal; Sitosterols

To study the chemical constituents in the aerial part of Thalictrum atriplex.. Chromatography and spectral analysis were used to isolate and elucidate the constituents.. Six compounds were isolated from the aerial part of Thalictrum atriplex, and elucidated as protocatechuic acid, caffeic acid, p-coumaric acid, kaempferol, beta-sitosterol and N-methylcorydaldine.. They are all isolated from the plant for the first time.

Topics: Caffeic Acids; Coumaric Acids; Drugs, Chinese Herbal; Flavonoids; Hydroxybenzoates; Kaempferols; Plants, Medicinal; Propionates; Sitosterols; Thalictrum