gamma-sitosterol and naringenin

To identify the active constituents, core targets, immunomodulatory functions and potential mechanisms of Dizhi pill (DZP) in the treatment of myopia. The active constituents and drug targets of DZP were searched in the TCMSP, Herb databases and correlational studies. The targets of myopia were searched in the TTD, Genecards, OMIM and Drugbank databases. Gene expression profile data of GSE136701 were downloaded from the GEO database and subjected to WGCNA and DEG analysis to screen for significant modules and targets of myopia. Intersectional targets of myopia and DZP and core targets of myopia were analyzed through the String database. The GO and KEGG enrichment analyses of the interested targets were conducted. Cibersort algorithm was used for immune infiltration analysis to investigate the immunomodulatory functions of DZP on myopia. Autodock was used to dock the important targets and active constituents. Eight targets (STAT3, PIK3CA, PIK3R1, MAPK1, MAPK3, HSP90AA1, MIP, and LGSN) and 5 active constituents (Quercetin, Beta-sitosterol, Diincarvilone A, Ferulic acid methyl ester, and Naringenin) were identified from DZP. In pathways identified by the GO and KEGG enrichment analyses, "ATP metabolic process" and "AGE-RAGE diabetes complication signaling" pathways were closely related to the mechanisms of DZP in the treatment of myopia. Molecular docking showed that both the intersectional targets and core targets of myopia could bind stably and spontaneously with the active constituents of DZP. This study suggested that the mechanisms of DZP in the treatment of myopia were related to active constituents: Quercetin, Beta-sitosterol, Diincarvilone A, Ferulic acid methyl ester and Naringenin, intersectional targets: STAT3, PIK3CA, PIK3R1, MAPK1, MAPK3, and HSP90AA1, core targets of myopia: MIP and LGSN, AGE-RAGE signaling pathway, positive regulation of ATP metabolic process pathway and immunomodulatory functions.

Topics: Adenosine Triphosphate; Computational Biology; Drugs, Chinese Herbal; Humans; Molecular Docking Simulation; Myopia; Quercetin; Transcription Factors

A phytochemical study of Ficus thonningii has led to the isolation of two previously unreported compounds, thonningiiflavanonol A and thonningiiflavanonol B together with 16 known compounds: shuterin, naringenin, syringic acid, p-hydroxybenzoic acid, genistein, 5,7,3',4',5'-pentahydroxyflavanone, luteolin, methylparaben, aromadendrin, garbanzol, dihydroquercetin, 5,7,3'-trihydroxyflavanone, β-sitosterol, sitosterolglucoside, lupeol acetate, and taraxerol. Their structures were elucidated on the basis of spectroscopic data. The new compounds and extracts displayed potent antioxidant activity.

Topics: Antioxidants; Ficus; Flavanones; Flavonoids; Gallic Acid; Genistein; Luteolin; Magnetic Resonance Spectroscopy; Molecular Structure; Oleanolic Acid; Parabens; Plant Bark; Plant Extracts; Plant Roots; Plant Stems; Quercetin; Sitosterols

To investigate the chemical constituents from the young fruits of Citrus maxima cv. Shatian.. The chemical constituents were isolated and purified by silica gel column chromatography and recrystallization, and their structures were identified on the basis of physicochemical properties and spectral analysis.. Seven compounds were isolated and identified as naringenin (I), marmin (II), naringin (III), β-sitosterol (IV) 5,7-dihydroxylcoumarin (V) 1, 3,5-trihydroxyhenzene (VI) and xanthotoxol (VII).. Except compound III, all compounds are isolated from the young fruits of Citrus maxima cv. Shatian for the first time.

Topics: Citrus; Flavanones; Fruit; Phytochemicals; Sitosterols

Six compounds were isolated from Rhizoma Heterosmilacis Japonicae. They were identified as beta-sitosterol (I) dau-costerol (II),3,3',5,5'-tetrahydroxy-4'-methoxystilbene (III), naringenin (IV), alpha-L-butylsorbopyranoside (V) and macrozamin (VI). The compounds III, IV, V and VI were isolated from this genus for the first time.

Topics: Flavanones; Liliaceae; Malpighiaceae; Methylazoxymethanol Acetate; Molecular Structure; Plant Tubers; Plants, Medicinal; Sitosterols; Spectrophotometry, Ultraviolet

In this paper three compounds isolated from Daphne koreane are described. Their structures have been identified as beta-sitosterol, naringenin and 7,8-dimethoxycoumarin on the basis of spectral data and chemical reactions.

Topics: Anti-Ulcer Agents; Coumarins; Drugs, Chinese Herbal; Flavanones; Flavonoids; Sitosterols