gamma-sitosterol and lyoniside

Beta-sitosterol (BSS) and its glycoside (BSSG) are sterol molecules which are synthesized by plants. When humans eat plant foods phytosterols are ingested, and are found in the serum and tissues of healthy individuals, but at concentrations orders of magnitude lower than endogenous cholesterol. Epidemiological studies have correlated a reduced risk of numerous diseases with a diet high in fruits and vegetables, and have concluded that specific molecules, including b-carotene, tocopherols, vitamin C, and flavonoids, confer some of this protective benefit. However, these epidemiologic studies have not examined the potential effect that phytosterols ingested with fruits and vegetables might have on disease risk reduction. In animals, BSS and BSSG have been shown to exhibit anti-inflammatory, anti-neoplastic, anti-pyretic, and immune-modulating activity. A proprietary BSS:BSSG mixture has demonstrated promising results in a number of studies, including in vitro studies, animal models, and human clinical trials. This phytosterol complex seems to target specific T-helper lymphocytes, the Th1 and Th2 cells, helping normalize their functioning and resulting in improved T-lymphocyte and natural killer cell activity. A dampening effect on overactive antibody responses has also been seen, as well as normalization of the DHEA:cortisol ratio. The re-establishment of these immune parameters may be of help in numerous disease processes relating to chronic immune-mediated abnormalities, including chronic viral infections, tuberculosis, rheumatoid arthritis, allergies, cancer, and auto-immune diseases.

Topics: Adjuvants, Immunologic; Animals; Cats; HIV Infections; Humans; Immune Tolerance; Sitosterols; Tuberculosis, Pulmonary

All available treatments for anogenital warts have substantial failure rates. An immunomodulating treatment that enhances the patient's own immunity could be valuable as an adjuvant to conventional methods.. About 123 patients were enrolled in this study and were randomized either to undergo only cryotherapy every 3 weeks (n = 60 patients) or to undergo cryotherapy and to receive a mixture containing 20 mg sterol and 0.2 mg sterolin (BSS-BSSG mixture), known for its immunomodulating properties (n = 63).. A complete response after 3 months was demonstrated by 18.3% of the patients in the first group and 30.2% of the second group, while 61.7% of the first group and 79.4% of the second group were lesion free at the end of the 6-month follow-up period. Cox regression analysis of the time until response showed a significant advantage to the mixture treatment group (hazard ratio 2.76, 95% confidence interval 1.61-5.67).. The study was not placebo controlled.. The BSS-BSSG mixture gave promising results as an adjuvant to cryotherapy and may be used in patients with refractory warts.

Topics: Adult; Combined Modality Therapy; Condylomata Acuminata; Cryotherapy; Female; Glucosides; Humans; Male; Middle Aged; Sitosterols; Young Adult

To evaluate the adjuvant effect of beta-sitosterol and its glucoside in the treatment of culture proven pulmonary tuberculosis (PTB).. A blinded randomised placebo-controlled trial in culture proven drug sensitive PTB. Patients were hospitalised for the duration of treatment and evaluated at monthly intervals with regard to sputum culture positivity, chest radiography, weight gain, Mantoux test response, routine haematology and liver functions. STATISTICAL EVALUATION: General linear models for repeated measures (SAS GLM package) compared the interaction effects, group effects and time effects of findings in 19 patients receiving sitosterols with those in 18 patients receiving a placebo (talcum powder). Absolute values and change from baseline values were evaluated, although only the latter are reported.. Weight gain was significantly greater in the sitosterol group (mean weight gain 8.9 kg) than the placebo group (mean gain 6.1 kg) (P = 0.0023 group effects; P = 0.0001 for time effects). Speed of achieving culture negativity, radiological improvement and induration on Mantoux testing was similar in the two groups. Change in lymphocyte counts from baseline was significantly higher in the sitosterol group (P = 0.0001 and P = 0.0001 for group and time effects) as was the increase in eosinophil counts (P = 0.0001 and P = 0.0137 for group and time effects).. The study has shown significantly improved weight gain and higher lymphocyte and eosinophil counts in PTB patients receiving sitosterols in addition to an efficacious antituberculosis regimen. Sitosterols and their possible mode of action should now be evaluated in larger numbers of tuberculosis patients and in diseases with a similar immunopathogenesis.

Topics: Adjuvants, Immunologic; Adult; Antitubercular Agents; Humans; Leukocyte Count; Male; Sitosterols; Tuberculosis, Pulmonary; Weight Gain

Three new compounds (

Topics: Animals; Anti-Infective Agents; Drug Evaluation, Preclinical; Insecticides; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Molecular Structure; Moths; Plant Extracts; Sitosterols; Viburnum

The phytochemical investigation of both chloroform and ethyl acetate extracts of

Topics: Asteraceae; Centaurea; Flavonoids; Glucosides; Molecular Structure; Nitriles; Plant Extracts; Sitosterols; Spectrum Analysis

Salvia aethiopis is a perennial plant native to Eurasia and known by the common names Mediterranean sage or African sage. This plant has been used in Iranian medicine as a carminative and tonic. The ethanolic extract of aerial part of S. aethiopis exhibited moderate to weak activity towards delta and kappa opioid receptors (46.3 and 45.3% displacement, respectively). Chromatographic purification of the ethanolic extract on silica gel column led to isolation of one new: 3α-hydroxy-8α-acetoxy-13,14,15,16-tetranorlabdan-12-oic acid (I) and three known compounds: sesquiterpene spathulenol (II), β-sitosterol (III) and β-sitosterol-3-O-glucoside (IV). The structures of all isolated compounds were elucidated by their NMR (1D and 2D) and MS spectral data. All the fractions and isolated compounds were tested for cannabinoid and opioid receptor binding assays.

Topics: Drug Evaluation, Preclinical; Iran; Magnetic Resonance Spectroscopy; Molecular Structure; Plant Components, Aerial; Plant Extracts; Plants, Medicinal; Receptors, Cannabinoid; Receptors, Opioid, delta; Receptors, Opioid, kappa; Salvia; Sesquiterpenes; Sitosterols

Cichorium intybus L. (Asteraceae family) is a world-wide grown plant known as chicory. In traditional medicine, this plant is used as diuretic, anti-inflammatory, digestive, cardiotonic and liver tonic. Chromatographic purification of the supercritical fluid extract of aerial parts of C. intybus on silica gel column led to isolation of three compounds: new compound, 28β-hydroxytaraxasterol (I), and two known compounds usnic acid (II) and β-sitosterol (III). Purification of the ethanolic extract of aerial parts of this plant on silica gel column chromatography yielded four compounds: 1,3-dioleylglycerate (IV), sitoindoside II (V), 11β-13-dihydrolactucin (VI) and β-sitosterol-3-O-glucoside (VII). The structures of the isolated compounds were determined by their 1D, 2D NMR and MS spectral data. All the fractions and isolated compounds were tested for cannabinoid and opioid receptor binding, as well as antibacterial, antifungal and antimalarial activities. Compound I showed moderate activity (60.5% displacement) towards CB1 receptor.

Topics: Anti-Bacterial Agents; Antifungal Agents; Antimalarials; Cichorium intybus; Drug Evaluation, Preclinical; Glucosides; Magnetic Resonance Spectroscopy; Medicine, Traditional; Molecular Structure; Plant Components, Aerial; Plant Extracts; Plants, Medicinal; Receptor, Cannabinoid, CB1; Receptors, Cannabinoid; Receptors, Opioid; Sitosterols

Lung cancer is the leading cause of cancer related deaths both in developed and developing countries. Since majority of the existing therapeutic methods harms both normal and malignant cells, a viable alternative is to switch into safe and beneficial traditional medicinal plants. Hence the present study was framed to identify selective anti-lung cancer agents from the medicinal plant Grewia tiliaefolia (GT). Cell viability experiments showed that benzene extract of GT (BGT) leaf effectively inhibited the growth of A549 cells, while being non-toxic to normal human lung L132 and PBMC cells. Ames and comet assays demonstrated that BGT is of non-mutagenic and non-genotoxic nature in untransformed cells. The hematological and histopathological profiles of the in vivo acute and sub-acute toxicity studies demonstrated that BGT is safe and tolerable. Importantly, western blot analysis and Annexin V-FITC staining confirmed that BGT promotes mitochondrial dependent apoptotic cell death in A549 cells by arresting cell cycle at G2/M phase. Bio-assay guided fractionation revealed the presence of phytosteols (β-sitosterol and daucosterol) which significantly inhibited the growth of A549 cells both alone and in combination. This study warrants that these phytosterols in alone or in combination can be considered as safe and potential drug candidates for lung cancer treatment.

Topics: Apoptosis; Cell Cycle; Cell Line, Tumor; Grewia; Humans; Sitosterols

To study the chemical constituents from the fleshy stems of Cynomorium songaricum.. The chemical constituents were isolated and purified by chromatography on MCI, silica gel, Sephadex LH-20 columns, etc. The structures of compounds were elucidated by physicochemical property and spectral analyses.. 15 compounds were isolated and identified as choerospondin( 1), isolariciresinol-4-O-β-D-glucopyranoside( 2), lutelin-7-O-β-D-glucopyranoside( 3), 3, 4-dihydroxyphenethylacetate( 4),maslinic acid( 5),catechin ( 6),ursolic acid( 7),gentisic acid( 8),mannitol( 9), phloroglucinol( 10), β-sitosterol( 11), daucosterol( 12), rutin( 13), cetylic acid( 14) and epicatechin( 15).. Compounds 4,5,8,9 and 10 are obtained from this plant for the first time.

Topics: Catechin; Cynomorium; Lignin; Naphthols; Phloroglucinol; Sitosterols; Triterpenes; Ursolic Acid

To investigate the chemical constituent from the roots of Gentiana straminea.. The constituents were separated by microporous resin,silica gel,Sephadex LH-20 and preparative column chromatography and their structures were elucidated by NMR and MS spectral methods.. Twelve chemical constituents were isolated from the roots of Gentiana straminea and their structures were identified as daucosterol( 1),β-sitosterol( 2),ursolic acid( 3),sweroside( 4),swertiamarin( 5),gentiopicroside( 6),6’-O-acetyl-gentiopicroside( 7),6’-O-β-D-glucopyranosyl-sweroside( 8),protocatech uic aldehyde( 9),protocatechuic acid( 10),methyl gallate( 11) and dibutyl phthalate( 12).. The compounds 8,9,10,11 and 12 are obtained from this plant for the first time.

Topics: Gentiana; Iridoid Glucosides; Magnetic Resonance Spectroscopy; Medicine, Tibetan Traditional; Plant Roots; Pyrones; Sitosterols; Triterpenes; Ursolic Acid

To study the chemical constituents from the whole plant of Delphinium caeruleum.. The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures of the isolated compounds were elucidated by spectroscopic analysis and physicochemical properties.. Twelve compounds were isolated and purified from the ethanol extract of Delphinium caeruleum. They were identified as β-sitosterol( 1),kaempferol( 2),quercetin( 3),isovanillic acid( 4),apigenin( 5),luteolin( 6),8-methoxy-5,7,3’,4’-tetrahydroxy-flavone( 7),β-daucosterol( 8),kaempferol-3-O-β-D-glucoside( 9),3,5-dihydroxy-4’-methoxyflavone-7-yl-O-β-D-glucopyranosyl-( 1→4)-α-L-rhamnopyranoside( 10),rutin( 11) and sucrose( 12).. Compounds 1 ~ 12 are isolated from this plant for the first time.

Topics: Apigenin; Delphinium; Glucosides; Kaempferols; Luteolin; Quercetin; Rutin; Sitosterols

To study the chemical constituents from Mitrasacme pygmaea.. The compounds were isolated and purified by column chromatography and their structure were identified by NMR and MS,and comparison spectral data with literature.. Eleven compounds were isolated and identified as tricin-7-O-β-D-glucopyranoside( 1),massonianoid A( 2),kaempferol( 3),cinnamic acid( 4),quercetin( 5),tiliroside( 6),tricin( 7),β-sitosterol( 8),adenosine( 9),α-tocopherolquinone( 10)and β-daucosterol( 11).. All the compounds are isolated from this genus for the first time.

Topics: Flavonoids; Kaempferols; Magnoliaceae; Quercetin; Sitosterols

To study the chemical constituents of stalk form Pottsia laxifora.. The chemical constituents were isolated by various chromatography methods,and the structures were elucidated by spectroscopic methods,physical and chemical properties.. Twelve compounds were isolated and their structures were identified as β-sitosterol( 1),salicylic acid( 2),resorcinol( 3),4-hydroxybenzoic acid( 4),protocatechuic acid( 5),vanillic acid( 6),syringic acid( 7),scopoletin( 8),cleomiscosin D( 9),( +)-lyoniresinol( 10),(-)-loliolide( 11) and daucosterol( 12).. Compounds 1 ~ 11 are isolated from this plant for the first time.

Topics: Coumarins; Gallic Acid; Hydroxybenzoates; Magnoliaceae; Scopoletin; Sitosterols; Vanillic Acid

To study the chemical consituents of Hypericum ascyron.. The constituents were isolated and purified by chromatography on silica gel; the structure of the compound was determined by MS and NMR spectral analysis.. On the basis of spectroscopic analysis and comparison with the reported data, they were identified as hyperoside( 1),hypercalin B( 2),hypercalin C( 3),1,7-dihydroxyxanthone( 4),2,3-dimethoxyxanthone( 5),1-hydroxy-7-methoxyxanthone( 6),rutin( 7),kaempferol( 8),toxyloxanthone B( 9),quercetin( 10),quercitrin( 11),β-daucosterol( 12) and β-sitosterol( 13).. Compounds 2,3,6 and 9 are obtained from this plant for the first time.

Topics: Acetates; Hypericum; Kaempferols; Quercetin; Rutin; Sitosterols

To study the non-alkaloid chemical constituents of Macleaya cordata.. Alcohol extraction and liquidliquid partitionmethods were used to extract the chemical constituents. Silica gel,reverse-phase octadecylsilyl( ODS), and Sephadex LH-20 column chromatographic methods were applied for isolation and purification. Spectroscopic methods including MS and NMR were used to determine their structures.. Eleven non-alkaloid compounds were isolated from the fruits of Macleaya cordata, and their structures were identified as 3-( 3,4-dihydroxy) phenylpropanoic acid methyl ester( 1),ferulic acid( 2),1-octacosanol( 3),syringic acid( 4),p-hydroxy-benzoic acid( 5),p-coumaric acid( 6),quercetin-3-O-β-D-glucoside( 7),N-p-coumaroyl tyramine( 8),10-eicosenoic acid( 9) and β-sitosterol( 10) and daucosterol( 11).. Compounds 1,3 ~9 are isolated from Macleaya cordata for the first time.

Topics: Alkaloids; Coumaric Acids; Gallic Acid; Glucosides; Papaveraceae; Quercetin; Sitosterols; Tyramine

To study the chemical constituents of Rubus stans.. The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures were identified on the basis of spectroscopic analysis and physicochemical properties.. Eleven compounds were isolated and purified from the ethanol extract of Rubus stans. They were identified as β-sitosterol( 1),betulinic acid( 2),euscaphic acid( 3),ursolic acid( 4),corosolic acid( 5),kaempferol( 6),quercetin( 7),2α,3β,19α,23-tetrahydroxy-urs-12-en-28-oic acid( 8),β-daucosterol( 9),quercetin-3-O-β-D-glucoside( 10) and kaempferol-3-O-β-D-6-O-( p-hydroxycinnamoyl)-glucopyranoside( 11).. All the compounds are isolated from this plant for the first time.

Topics: Betulinic Acid; Kaempferols; Pentacyclic Triterpenes; Rubus; Sitosterols; Triterpenes; Ursolic Acid

To study the chemical constituents of the stems from Altingia chinensis.. The stems from Altingia chinensis were extracted with 95% ethanol for reflux,and the extract were evaporated. The chemical constituents were isolated by silica gel chromatography and Sephadex LH-20 column chromatography from petrol ether part and ethyl acetate part of extract. Their structures were identified on the basis of physico-chemical characters and spectroscopic analysis.. Eleven compounds were obtained from the stems from Altingia chinensis,which identified as myrsinene( 1),oleanonic aldehyde( 2),3β,23,28-trihydroxyolean-12-ene( 3),ursolic acid-3β-octadecanoate( 4),arjunglucoside Ⅱ( 5),β-sitosterol( 6),daucosterol( 7),trans-resveratrol-3-O-β-D-glucopyranoside( 8),ellagic acid 3,3’-dimethylether( 9),lyoniside( 10) and 3,3’-O-dimethylellagic acid-4’-O-α-L-rhamnoside( 11).. All the compounds are isolated from Altingia chinensis for the first time.

Topics: Ellagic Acid; Saxifragaceae; Sitosterols; Triterpenes; Ursolic Acid

To study the chemical constituents of the methylene chloride extract of Lonicerae Japonicae Flos.. The compounds were isolated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography and recrystallization. The structures were elucidated by the physical and chemical properties,MS,1H-NMR and13C-NMR spectroscopy.. Nine compounds were isolated and their structures were identified as β-sitosterol( 1),benzoic acid( 2),5-hydroxy-7,3’,4’-trimethoxyflavone( 3),tetrapedic acid B( 4),tricin( 5),hydnocarpin D( 6),6,7,10-trihydroxy-8-octadecenoic acid( 7),5’-methoxyhydnocarpin-D( 8) and daucosterol( 9).. Compound 2,4,7,8 are isolated from this plant,compounds 2,7,8 are isolated from Caprifoliaceae family for the first time.

Topics: Drugs, Chinese Herbal; Flavonoids; Lonicera; Plant Extracts; Sitosterols

Hedyotis caudatifolia Merr. et Metcalf. (HC), a folk medicine in Yao nationalities areas in China, was used to investigate the chemical constituents. Through silica gel and Sephadex LH-20 column chromatography, nine compounds were isolated and purified. By physical and chemical properties, IR, MS (EI-MS, high resolution EI-MS), 1D NMR ((1)H NMR, (13)C NMR) and 2D NMR (HSQC, (1)H-(1)H COSY, HMBC), their structures were identified as β-sitosterol (1), stigmasterol (2), scopolin (3), 2-hydroxy-1,7,8-trimethoxyanthracene-9,10-dione (4), oleanolic acid (5), ursolic acid (6), methyl barbinervate (7), β-daucosterol (8) and p-Hydroxybenzoic acid (9). These compounds were isolated from HC for the first time, and 4 a new anthraquinone whose biological activities are worth to be investigated in future. These compounds may contribute to the HC's pharmacological effects on treating diseases, and may be used as candidates for control index in establishing the quality control standard of HC.

Topics: Anthraquinones; China; Hedyotis; Magnetic Resonance Spectroscopy; Molecular Structure; Oleanolic Acid; Plants, Medicinal; Sitosterols; Stigmasterol; Triterpenes; Ursolic Acid

The chemical investigation of ethanolic extract from rhizomes of Cautleya spicata (Sm.) Baker (Zingiberaceae) has resulted in the isolation of eight compounds which were characterised as β-sitosterol (1), β-sitosterol β-D-glucoside (2), bergapten (3), zerumin A (4), (E)-labda-8(17),12-diene-15,16-dial (5), kaempferol (6), quercetin (7) and astragalin (8). All compounds were identified by spectroscopic and chemical methods. This paper describes the first phytochemical work on C. spicata.

Topics: Glucosides; Kaempferols; Molecular Structure; Phytochemicals; Plant Extracts; Quercetin; Rhizome; Sitosterols; Zingiberaceae

Sterol glycosyltransferases regulate the properties of sterols by catalyzing the transfer of carbohydrate molecules to the sterol moiety for the synthesis of steryl glycosides and acyl steryl glycosides. We have analyzed the functional role of TTG15/UGT80B1 gene of Arabidopsis thaliana in freeze/thaw and heat shock stress using T-DNA insertional sgt knockout mutants. Quantitative study of spatial as well as temporal gene expression showed tissue-specific and dynamic expression patterns throughout the growth stages. Comparative responses of Col-0, TTG15/UGT80B1 knockout mutant and p35S:TTG15/UGT80B1 restored lines were analyzed under heat and freeze stress conditions. Heat tolerance was determined by survival of plants at 42°C for 3 h, MDA analysis and chlorophyll fluorescence image (CFI) analysis. Freezing tolerance was determined by survival of the plants at -1°C temperature in non-acclimatized (NA) and cold acclimatized (CA) conditions and also by CFI analysis, which revealed that, p35S:TTG15/UGT80B1 restored plants were more adapted to freeze stress than TTG15/UGT80B1 knockout mutant under CA condition. HPLC analysis of the plants showed reduced sterol glycoside in mutant seedlings as compared to other genotypes. Following CA condition, both β-sitosterol and sitosterol glycoside quantity was more in Col-0 and p35S:TTG15/UGT80B1 restored lines, whereas it was significantly less in TTG15/UGT80B1 knockout mutants. From these results, it may be concluded that due to low content of free sterols and sterol glycosides, the physiology of mutant plants was more affected during both, the chilling and heat stress.

Topics: Acclimatization; Arabidopsis; Arabidopsis Proteins; Chromatography, High Pressure Liquid; Ecotype; Fluorescence; Freezing; Gene Expression Regulation, Plant; Genes, Plant; Glucosyltransferases; Hot Temperature; Lipid Peroxidation; Mutation; Phenotype; Promoter Regions, Genetic; Sitosterols; Stress, Physiological; Time Factors

To study the chemical constituents of fermented Antrodia camphorata powder.. 15 compounds were isolated from Antrodia camphorata by Silica gel column chromatography, ODS column chromatography, gel column chromatography, preparative liquid phase chromatography separation technique, as well as recrystallization.. On the basis of their physical and chemical properties and spectral data,their structures were identified as Ferulic acid (1), Inositol (2), β-Sitosterol (3),Vanillin (4),Vanillic acid (5), Butyric acid (6), Daucosterol (7), p-Hydroxycinnamic acid (8), Lauric acid (9), Inosine (10), Uridine (11), Adenine (12), D(+)-Sucrose (13), Arachidic acid (14) and Guanosine (15).. Compounds 1, 5, 6 and 8-15 are isolated from fermented powder for the first time.

Topics: Antrodia; Chromatography, Liquid; Coumaric Acids; Fermentation; Phytochemicals; Plants, Medicinal; Powders; Propionates; Sitosterols; Vanillic Acid

To study the chemical constituents from the leaves of "Chuju" Chrysanthemum morifolium.. All compounds were separated and purified by column chromatography over silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by spectral methods including 1H-NMR and 13C-NMR.. 21 compounds were isolated and identified as octa-cosyl alcohol (1), β-sitosterol (2), lupeol (3), α-amyrin (4), daucosterol (5), ineupatorolide B (6), syringin (7), chlorogenic acid (8), petasiphenol (9), physcion (10), acacetin (11), eupatilin (12), quercetin (13), diosmetin (14), luteolin (15), apigenin (16), apigenin- 7-O-β-D-glucopyranoside (17), quercetin-3-O-β-D-glucopyranoside (18), luteolin-7-O-β-D-gluco pyranoside (19), apigenin-7-O-β-D- neospheroside (20), and acacetin-7-O-β-D-glucoside (21).. Compounds 1-12, 18 and 20 are isolated from this plant for the first time. Compounds 10, 13, 14, 15 and 16 have shown strong antioxidant activities by DPPH · scavenging activity better than Vit C.

Topics: Antioxidants; Apigenin; Chlorogenic Acid; Chrysanthemum; Flavones; Flavonoids; Glucosides; Luteolin; Phenylpropionates; Phytochemicals; Plant Leaves; Plants, Medicinal; Quercetin; Sitosterols

To investigate the chemical constituents of peanut hull.. Several chromatography methods such as silica gel and Sephadex LH-20 combined with recrystallization were applied to isolate the compounds. Based on spectrum technologies (MS,1H-NMR and 13C-NMR) and physico-chemical methods, structures of isolated compounds were identified.. Twelve compounds were isolated and elucidated as luteolin (1), diosmetin (2), 5,7,3',4'-tetrahydroxy-8-prenyflavone (3),5,7,3'-trihydroxy-4'- methoxy-8-prenylflavone(4), eriodicrtyol (5), racemoflavone (6), hydnocarpin (7), 5,7-dihydroxy chromone (8), 5-hydroxy-chromone- 7-O-β-D-glucoside (9), ferulic acid (10), β-sitosterol (11) and daucosterol(12).. Except compounds 1, 5 and 8, all compounds are obtained from peanut hull for the first time.

Topics: Arachis; Coumaric Acids; Flavonoids; Luteolin; Phytochemicals; Seeds; Sitosterols

To investigate the chemical constituents of Melissa officinalis leaves.. The chemical constituents were separated by silica gel column chromatography and their structures were determined by spectroscopic experiments.. 13 compounds were isolated and identified as protocatechuyl aldehyde(1), serratagenic acid(2), vanillin(3), 2α,3β-dihydroxy-urs-12-en-28-oic acid(4), ursolic acid(5), oleanolic acid(6), daucosterol(7),2α,3β,23,29-tetrahydroxyolean-12-en-28-oic acid-29-O-β-D-gluco- pyranoside(8), luteolin(9) rosmarinic acid(10), luteolin-7-O-β-D-glucoside (11), β-stitosterol(12) and palmitic acid(13).. Compounds 1 ~ 8 are separated from this plant for the first time and compounds 1-4 and 8 are isolated from this genus for the first time.

Topics: Benzaldehydes; Cinnamates; Depsides; Melissa; Oleanolic Acid; Palmitic Acid; Phytochemicals; Plant Extracts; Rosmarinic Acid; Sitosterols; Triterpenes; Ursolic Acid

To study the chemical constituents of Suaeda glauca.. The chemical constituents were isolated and purified with several separation and purification techniques. Their structures were identified by physicochemical properties and various spectroscopic methods.. Ten compounds were isolated from the ethyl acetate fraction as lignoceric acid (1), β-amyrin-n-nonyl ether(2), β-sitosterol(3), β-daucosterol(4), quercetin(5), luteolin(6), luteolin-7-O-β-D-glucoside(7), isorhamnetin(8), scopoletin (9) and stigmasterol(10).. Compounds 1, 2, 6, 7, 8, 9 and 10 are isolated from Suaeda genus for the first time and compounds 3 - 5 are isolated from this plant for the first time.

Topics: Acetates; Chenopodiaceae; Glucosides; Luteolin; Oleanolic Acid; Phytochemicals; Plant Extracts; Quercetin; Sitosterols; Stigmasterol

To study the chemical constituents of Claoxylon indicum stems.. The chemical constituents were isolated, purified and identified by various chromatographic and spectral techniques.. Seven compounds including oleanolic acid (1), 6-amyrin(2), 7-ketositosterol(3), scopoletin(4), β-sitosterol-β-glucoside(5), L-pyroglutamic acid (6) and β-sitosterol (7) were isolated from ethanol extract of this plant stems.. Compounds 1 - 6 are obtained from this plant for the first time.

Topics: Euphorbiaceae; Oleanolic Acid; Phytochemicals; Plant Stems; Sitosterols

To investigate the chemical constituents of the processed products of Aconitum Vilmorinian Radix.. The constituents were isolated by repeated column chromatography over silica gel, alumina and RP-C18 as well as recrystallization. The structures were elucidated on the basis of spectral analysis and physicochemical properties.. Ten compounds were obtained from the methanol extract, and they were identified as yunaconitine (1), 8-deacetyl-yunaconitine (2), geniculatine C (3), vilmorrianine B (4), vilmorrianine C(5), vilmorrianine D (6), talatisamine (7), β-sitosterol (8), β-daucosterol (9) and β-sitosterol acetate (10).. All compounds are obtained from the processed products of Aconitum Vilmoriniani Radix for the first time.

Topics: Aconitine; Aconitum; Phytochemicals; Plant Roots; Sitosterols

To study the chemical constituents of aerial parts of Paris polyphylla var. chinensis .. Aerial parts of Paris polyphylla var. chinensis was extracted with 95% EtOH, and separated and purified by silica gel, RP 18 and Sephadex LH-20 col- umn chromatography. The structures were identified by spectroscopic analysis.. A total of ten compounds were isolated and iden- tified as β-sitosterol (1) ergosta-7, 22-dien-3-one (2), β-ecdysone (3), kaempferol (4), daucosterol (5) luteolin (6) calonysterone (7), luteolin-7-O-glucoside (8), quercetin (9), and 3β, 5α, 9α-trihydroxyergosta-7, 22-dien-6-one (10).. Compounds 2,6 and 10 are isolated from Paris polyphylla var. chinensis for the first time.

Topics: Ecdysterone; Flavones; Glucosides; Kaempferols; Liliaceae; Phytochemicals; Plant Components, Aerial; Plant Extracts; Plants, Medicinal; Quercetin; Sitosterols

To study the chemical constituents from the aerial part of Aconitum brachypodum.. The constituents were isolated and purified by silica gel, activated alumina and Sephadex LH-20 column chromatography. their structures were elucidated on the basis of spectral data and physiochemical evidence.. Eleven compounds were isolated from 80% ethanol extract and identified as secokaraconitine (1), brachyaconitines A (2), C (3), talatisamine (4), hypaconitine (5), songrine (6), bullatine A (7), 7-carbony sitosterone (8), lupeol (9), β-sitosterol (10) and daucosterol (11).. All compounds are isolated from the aerial part of Aconitum brachypodum for the first time.

Topics: Aconitum; Dextrans; Pentacyclic Triterpenes; Plant Components, Aerial; Sitosterols

To separate and identify the chemical constituents from the stem of Schisandra chinensis.. Various chromatographic techniques were used to separate and purify the chemical constituents from 95% ethanol extraction of the stem of Schisandra chinensis. Their structures were elucidated based on the physico-chemical properties and spectral data.. Ten compounds were obtained and elucidated as (+)-deoxyschizandrin (1), γ-schizandrin (2), wuweizisu C (3), gomisin N (4), schizandrin (5), anwuweizic acid (6), (-)-dihydroguaiaretic acid (7), tetradecanoic acid (8), β-sitosterol (9) and daucosterol (10).. Compounds 6-8 are obtained from the stem of Schisandra chinensis for the first time.

Topics: Cyclooctanes; Guaiacol; Lignans; Phytochemicals; Plant Stems; Polycyclic Compounds; Schisandra; Sitosterols

To study the anti-tumor chemical components of the pericarps of Juglans mandshurica.. The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin, silica gel, Sephadex LH-20 columns and recrystallization. The structures were elucidated on the basis of physicochemical properties and NMR spectral data analysis.. From the pericarps of Juglans mandshurica, twelve compounds were separated and identified as 3-methoxy juglone(1), 3-ethoxy juglone(2), 1,8-di-hydroxy anthraquinone (3), juglone (4), 2α, 3α, 19α-trihydroxy ursolic acid (5), 1α, 3β-dihydroxy-olean-18-ene (6), methyl gallate (7), pterocarine(8), quercetin(9), kaempferol(10), daucosterol(11), and β-sitosterol(12).. Compounds 1 - 3 and 6 are isolated from the pericarps of Juglans mandshurica for the first time. Compounds 5 and 7 are isolated from Juglans genus for the first time.

Topics: Anthraquinones; Antineoplastic Agents, Phytogenic; Drugs, Chinese Herbal; Gallic Acid; Juglans; Kaempferols; Naphthoquinones; Phytochemicals; Seeds; Sitosterols; Triterpenes; Ursolic Acid

To study the chemical constituents from the supercritical CO2 extraction of Schisandra chinensis.. The compounds were separated and purified by conventional column chromatography and their structures were identified by spectroscopic methods.. Nine compounds were isolated from the supercritical CO2 extraction of Schisandra chinensis, and their structures were identified as chrysophanol(1),schisandrin B(2), β-sitosterol(3), schisandrin C(4),schisandrol A(5), angeloylgomisin H(6), daucosterol(7) 1, 5-dimethyl citrate (8), and shikimic acid (9).. Compounds 1, 8 and 9 are isolated from Schisandra chinensis for the first time,and compound 1 as an anthraquinone is isolated from this genus for the first time.

Topics: Cyclooctanes; Lignans; Phytochemicals; Polycyclic Compounds; Schisandra; Sitosterols

To study the chemical constituents from the seeds of Euryale ferox.. The chemical constituents were isolated by silica gel column, Sephadex LH-20 and their structures were identified by physico-chemical and spectral analysis.. Seven compounds were purified from the 95% ethanol extract. These constituents were elucidated as protocatechuic acid (1), gallic acid (2), gallic acid ethyl ester(3),5 ,7-dihydroxychromone(4), β-sitosterol(5), daucosterol(6), and 5,7-dihydroxy-6,4'-dimethoxyflavone(7), respectively.. All compounds are isolated from this plant for the first time.

Topics: Gallic Acid; Hydroxybenzoates; Nymphaeaceae; Phytochemicals; Seeds; Sitosterols

To study chemical constituents of the roots of Lonicera macranthoides.. The chemical constituents were isolated and purified by means of several chromatographic techniques and their structures were elucidated by spectroscopic methods.. Seven compounds were isolated and identified as ribenol (1), excoecarin C (2), 18-hydroxy-13-epi-manoyloxide (3), asiatic acid (4), oleanolic acid (5), β-sitosterol (6) and β-daucosterol (7).. Compounds 1-4 are obtained from this genus for the first time. Compound 5 is obtained from this plant for the first time. All the compounds are found from the roots of Lonicera mac- ranthoides for the first time.

Topics: Lonicera; Oleanolic Acid; Pentacyclic Triterpenes; Phytochemicals; Plant Roots; Sitosterols

To study the chemical constituents of Ajuga nipponensis.. The chemical constituents were isolated by repeated silica gel column chromatography and their structures were elucidated by phyisochemical properties and spectral analysis.. Ten compounds were isolated and identified as:hexadecanoic acid(1), ajuforrestin A(2), beta-sitosterol(3), acacetin(4), apigenin(5), ajugamacrin B(6), ursolic acid(7), beta-ecdysone(8), 8-acetylharpagide(9) and daucosterol(10).. Compounds 1-7 and 10 are isolated from this plant for the first time.

Topics: Ajuga; Apigenin; Flavones; Molecular Structure; Palmitic Acid; Plants, Medicinal; Sitosterols; Spectrophotometry, Ultraviolet

To study the chemical constituents of Pouzolzia zeylanica.. Many chromatography means were used in separation and purification, and the structures of all compounds were identified by the means of spectroscopic analysis and physicochemical properties.. 14 compounds were elucidated as: beta-sitosterol (1), daucosterol (2), oleanolic acid (3), epicatechin (4), alpha-amyrin (5), eugenyl-beta-rutinoside (6), 2alpha, 3alpha, 19alpha-trihydroxyurs-12-en-28-oic (7), scopolin (8), scutellarein-7-O-alpha-L-rhamnoside (9), scopoletin (10), quercetin (11), quercetin-3-O-beta-D-glucoside (12), apigenin (13), 2alpha-hydroxyursolic acid (14).. All compounds are obtained from this plant for the first time.

Topics: Catechin; Chromatography, Thin Layer; Glycosides; Oleanolic Acid; Sitosterols; Urticaceae

To study the chemical constituents from Cyathea spinulosa.. Compounds were isolated by chromatographic techniques. Their structures were elucidated by spectral methods.. Eight compounds were isolated from the ethanol extract of Cyathea spinulosa and identified as stigmast-4-ene-3,6-dione (1), stigmast-3,6-dione (2), ergosterol (3), protocatechuic aldehyde (4), 1-O-beta-D-glucopyranosyl-(2S,3R,4E,8Z)-2-[(2-hydroxyoctadecanoyl) amido]-4,8- octadecadiene-1,3-diol (5), (2S,3S, 4R)-2-[(2'R) -2'-hydroxytetracosanoylamino]-1,3,4-octadecanetriol (6), beta-sitosterol (7), daucosterol (8).. Compounds 1-6 are isolated from this plant for the first time.

Topics: Benzaldehydes; Catechols; Cholestenones; Ergosterol; Ferns; Molecular Structure; Plant Stems; Sitosterols

Local communities in Malaysia consume Pereskia bleo Kunth. (Cactaceae) leaves as raw vegetables or as a concoction and drink as a tea to treat diabetes, hypertension, rheumatism, cancer-related diseases, inflammation, gastric pain, ulcers, and for revitalizing the body.. To evaluate anti-nociceptive activity of the extracts and vitexin, isolated for the first time in this species, in two analgesic models; formalin-induced licking and acetic acid-induced abdominal writhing.. Three and a half kilos of P. bleo leaves were extracted using Soxhlet apparatus with ethanol for 72 h. The crude ethanol extract was treated with activated charcoal overnight and subjected to a liquid-liquid partition yielding hexane, dichloromethane, ethyl acetate and butanol extracts. All extracts, including the crude ethanol and vitexin isolated from the ethyl acetate partition were tested for peripheral anti-nociceptive activity using formalin test and acetic acid-induced abdominal writhing, besides having their acute toxicity assays performed.. The phytochemical analyses resulted in the isolation of vitexin (1), β-sitosterol glucoside (2) and β-sitosterol (3) isolated from the ethyl acetate, dichloromethane and hexane extracts, respectively. This is the first time vitexin and β-sitosterol glucoside are isolated from this species. The anti-nociceptive activities for all extracts were only moderate. Vitexin, which was isolated from the ethyl acetate extract did not show any activity in all models tested when used alone at the same concentration as it appears in the extract.. This study showed that all the extracts possess moderate anti-nociceptive activity. Vitexin is not the compound responsible for the anti-nociceptive effect in the ethyl acetate extract. Further investigations are needed to identify the compound(s) that might be responsible for the anti-nociceptive activity in this plant.

Topics: Acetic Acid; Analgesics; Animals; Apigenin; Behavior, Animal; Cactaceae; Formaldehyde; Male; Mice; Pain; Plant Extracts; Plant Leaves; Sitosterols; Toxicity Tests, Acute

To study the chemical constituents of Pileostegia viburnoides var. glabrescens.. The compounds were isolated and purified by various techniques. Their structures were determined by physicochemical properties and spectral analysis.. Five compounds were isolated and identified as friedelin (1), beta-sitosterol (2), umbelliferone (3), daucosterol (4) and skimmin (5).. All the compounds were isolated from this genus for the first time.

Topics: Magnetic Resonance Spectroscopy; Molecular Structure; Saxifragaceae; Sitosterols; Triterpenes; Umbelliferones

To study the chemical constituents of Exochorda racemosa.. Compounds were isolated and purified by silica gel, Sephadex LH-20, MCI gel and RP-18 column chromatography, and their structures were determined by spectroscopic analysis.. Twenty compounds were isolated and identified as N-p-coumaroyl-N'-caffeoylputrescine (1), sutherlandin trans-p-coumarate (2), apigenin 7-O-methylglucuronide (3), astragalin (4), nicotiflorin (5), kaempferol 3-neohesperidoside (6), rutin (7), apigenin (8), luteolin (9), linalool-1-oic acid (10), betulalbuside A (11), ursolic acid (12) , corosolic acid (13), gynuramide II (14), beta-sitosterol (15), daucosterol (16), uridine (17), adenosine (18), syringin (19), and trans4-hydroxycinnamic acid (20), respectively.. All compounds were obtained from this plant for the first time, moreover, 1 was reported as a new natural product, and 2 is a naturally rare cyanogenic glycoside.

Topics: Apigenin; Flavonoids; Glucosides; Glucuronides; Kaempferols; Luteolin; Magnetic Resonance Spectroscopy; Phenols; Phenylpropionates; Rosaceae; Sitosterols; Triterpenes; Ursolic Acid

To study the chemical constituents of the aerial part of Ligusticum jeholense.. The constituents were isolated by sillica gel column chromatography, Sephadex LH-20 column chromatography and their structures were elucidated by spectral analysis.. Seven compounds were separated from the EtOH extracts. Their structures were identified as psoralen (1), beta-sitosterol (2), daucosterol (3), kaempferol-3-O-(2",4"-di-E-p-coumaroyl)-alpha-L-rhamnoside (4), kaempferol-3-O-beta-D-galactoside (5), quercetin-3-O-beta-D-galactoside (6), sucrose (7).. Compounds 1, 4, 5 and 6 are isolated from the genus for the first time. Compounds 2, 3 and 7 are isolated from the aerial part of the plant for the first time.

Topics: Ethanol; Ficusin; Kaempferols; Ligusticum; Molecular Structure; Monosaccharides; Plant Components, Aerial; Plants, Medicinal; Quercetin; Sitosterols; Sucrose

To systematically study the chemical constituents in the roots of Gentiana dahurica.. Various column chromatographic techniques were used for isolation and purification. The structures were elucidated on the basis of spectral data (UV, IR, MS, NMR) and identified by comparing with the authentic substance.. Seven compounds were isolated and identified as: roburic acid (1), oleanolic acid (2), beta-sitosterol (3), daucosterol (4), gentiopicroside(5), swertiamarine (6), sweroside (7).. Compounds 1, 2 and 4 are isolated from this plant for the first time.

Topics: Gentiana; Molecular Structure; Oleanolic Acid; Plant Roots; Plants, Medicinal; Sitosterols; Spectrophotometry, Ultraviolet; Triterpenes

To study the chemical constituents of the Ficus microcarpa.. Isolation and identification were carried out by using various chromatography techniques and spectral methods.. Eight compounds were isolated. Their structures were identified as beta-amyrone (I), lupeol (II), lupeol acetate (III), maslinic acid (IV), epifriedelinol (V), stearic acid (VI), beta-sitosterol (VI), daucosterol (VI).. Compounds I, II, VI are isolated from this plant for the first time.

Topics: Ficus; Magnetic Resonance Spectroscopy; Molecular Structure; Pentacyclic Triterpenes; Plant Leaves; Sitosterols; Stearic Acids; Triterpenes

Five sesquiterpenes, dehydrocostus lactone (1), santamarine (5), beta-cyclocostunolide (6), 4alpha-hydroxy-4beta-methyldihydrocostol (7) and 10alpha-hydroxyl-artemisinic acid (9), along with four other compounds, beta-sitosterol (2), daucosterol (3), 5-hydroxymethyl-furaldehyde (4), and trans-syingin (8), were isolated and identified from the roots of Saussurea lappa (Compositae). Based on previous reports and our study, sesquiterpene derivatives are common and characteristic constituents of the genus Saussurea. Among the nine compounds obtained, 9 is a new sesquiterpene. It is an artemisinic acid derivative, whose structural skeleton has not been reported for Saussurea species before, but artemisinic acid is a common compound in another Compositae species, Artemisia annua. Dehydrocostus lactone (1) is present in high-content and is a possible bioprecursor of 10alpha-hydroxyartemisinic acid (9).

Topics: Furaldehyde; Plant Roots; Saussurea; Sesquiterpenes; Sitosterols

To investigate the chemical constituents of the stem and leaves of Tagetes erecta.. The materials extracted with ethanol were first purified with D101 resin and then separated by repeated silica gel column chromatography as well as recrystallization to get single compounds. The chemical structures of the compounds were elucidated on the basis of physicochemical properties, spectroscopic analysis and comparing with standard sample and literatures.. Six compounds were identified as 4'-methoxy-eupatolitin-3-O-glucoside (I), kaempferitrin (II), rutin (III), beta-sitosterol (IV), daucosterol (V) and gallic acid (VI).. Compounds I, II, III are isolated from the plant for the first time; the compounds IV, V, VI are isolated from the stem and leaves of the plant for the first time.

Topics: Ethanol; Gallic Acid; Kaempferols; Molecular Structure; Plant Leaves; Plant Stems; Resins, Synthetic; Rutin; Sitosterols; Tagetes

Silica gel column chromatography was used for the isolation and purification of the chemical constituents of the pericarp of Illicium macranthum. From dichloromethane-EtOAc (1:1) fraction and EtOAc fraction of the methanol extracts, eleven compounds were identified on the basis of chemical and spectral data. Two new compounds were elucidated to be 6-deoxyneomajucin (1) and 2-oxo-6-deoxyneomajucin (2), along with nine known compounds 6-deoxypseudoanisatin (3), pseudoanisatin (4), anisatin (5), pseudomajucin (6), protocatecheuic acid (7), shikimic acid (8), shikimic acid methylester (9), beta-sitosterol (10) and daucosterol (11). Compounds 1 and 2 are new majucin-type sesquiterpene lactones.

Topics: Drugs, Chinese Herbal; Fruit; Illicium; Lactones; Magnetic Resonance Spectroscopy; Molecular Structure; Plants, Medicinal; Sesquiterpenes; Shikimic Acid; Sitosterols; Spiro Compounds

To study the chemical constituents of Yao Medicine Cissus pteroclada.. The compounds were isolated and purified by column chromatography with silica gel, TLC and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis.. Six compounds were isolated and identified as beta-sitosterol (I), bergenin (II), 11-O-galloylbergenin (III), 11-O-(4-hydroxy benzoyl) bergenin (IV), gallic acid (V), daucosterol (VI).. Compounds III and NIV are obtained from the genus for the first time. All the compounds are isolated from this plant for the first time except the compound II.

Topics: Benzopyrans; Cissus; Gallic Acid; Molecular Structure; Plant Stems; Plants, Medicinal; Sitosterols; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet

To study the chemical constituents of the root tube from Pteroxygonum giraldii.. Column chromatography and spectral analysis were used to isolate and identify the constituents.. Ten compounds were isolated and identified as beta-sitosterol (I), beta-sitosterol glucoside (II), 4', 5,5', 7-tetrahydroxy-3'-methoxy-3'-O-alpha-L-arabinopyranosyl flavone (III), gallic acid (IV), myricetin (V), annulatin (VI), 5,5', 7-trihydroxy-2',3-dimethoxy-4'-O-beta-D-glucopyranosyl flavone (VII), 2', 5,5',7-tetrahydroxy -3-methoxy-4'-O-beta-D-glucopyranoside flavone (VIII), myricetin-3-O-alpha-L-rhamnopyranoside (IX) and myricetin-3,4'-dimethyl ether( X).. Compounds I, II, V, VIII and X are isolated from Pteroxygonum giraldii for the first time.

Topics: Flavonoids; Gallic Acid; Molecular Structure; Plant Roots; Plants, Medicinal; Polygonaceae; Sitosterols

From the methanolic extract of the roots of Clerodendrum philipinum, a new rearranged abietane diterpene (1) and eight known compounds were isolated by various chromatography methods. Their structures were identified by means of spectroscopic methods, including 1D- and 2D-NMR, as 17(15-->16),18(4-->3)-bisabeo-11,12,14,16-tetrahydroxy-3,5,8,11,13,15-abietahexaen-7-one (1), binankadsurin A, clerodenoside A, martynoside, acteoside, isoacteoside, astragalin, p3-sitosterol, and daucosterol. Binankadsurin A was found for the first time from a Clerodendrum species.

Topics: Abietanes; Chromatography; Clerodendrum; Glucosides; Kaempferols; Lignans; Magnetic Resonance Spectroscopy; Methanol; Molecular Structure; Phenols; Sitosterols

To study the chemical constituents from the antioxidant fraction of Neo-Taraxacum siphonathum.. Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated on the basis of chemical evidence and spectral analysis.. Ten compounds were isolated and identified from Neo-T. siphonathum, caffeic acid (1), chlorogenic acid (2), quercetin (3), luteolin (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin-3-O-alpha-D-arabinofuranoside (6), quercetin-3-O-alpha-D-arabinopyranoside (7), luteolin-7-O-beta-D-glucopyranoside (8), beta-sitosterol (9) and daucosterol (10).. Compounds 1-10 were isolated from this plant for the first time.

Topics: Caffeic Acids; Chlorogenic Acid; Glucosides; Luteolin; Magnetic Resonance Spectroscopy; Quercetin; Sitosterols; Taraxacum

To study the chemical constituents in the leaves and stems of Aconitum coreanum.. The isolation and purification of chemical constituents were carried out on silica gel and polyamide column chromatographic. Their structures were identified by physico-chemical properties and spectral analysis.. Five compounds were obtained and their structures were identified as guan-fu base I (1), guan-fu base R (2), beta-sitosterol (3), D-mannitol (4), daucosterol (5).. Compound 2 is a new compound. Compounds 1 and 3, 4 are isolated from the leaves and stems of A. coreanum for the first time.

Topics: Aconitum; Molecular Structure; Plant Leaves; Plant Stems; Sitosterols

To study the chemical constituents in roots of Saussurea lappa.. Isolation and purification were carried out by silica gel, Sephadex LH-20 and RP-18 column chromatography. The chemical structures of constituents were elucidated on the basis of spectral data.. Eleven compounds were isolated and identified as: 5,7-dihydroxy-2-methylchromone (1), p-hydroxybenzaldehyde (2), 3,5-dimethoxy-4-hydroxy-benzaldehyde (3), 3,5-dimethoxy-4-hydroxy-acetophenone (4), ethyl 2-pyrrolidinone-5(s)-carboxylate (5), 5-hydroxymethyl-furaldehyde (6), palmitic acid (7), succinic acid (8), glucose (9), daucosterol (10), beta-sitosterol (11).. Compounds 1, 2, 4, 5, 7, 9 were isolated from the genus Saussurea for the first time.

Topics: Benzaldehydes; Palmitic Acid; Plant Roots; Pyrrolidinones; Saussurea; Sitosterols

To study the chemical constituents of the fruit handles from Schizandra chinensis.. Compounds from the 85% ethanol extracts were isolated by silica gel, Sephadex LH-20, recrystal, etc., and their structures were identified by the spectral analysis and chemical evidence.. Eight compounds were isolated and identified as wuweizisu C (I), ganwuweizic acid(II), beta-sitosterol(III), gomisin A(IV), schizandrin(V), daucosterol(VI), wuweizisu A(VII), gamma-schizandrin (VIII).. Compounds I - VIII are isolated from the fruit handles of Schizandra chinensis for the first time.

Topics: Chromatography, Thin Layer; Cyclooctanes; Dioxoles; Fruit; Lignans; Magnetic Resonance Spectroscopy; Plants, Medicinal; Polycyclic Compounds; Schisandra; Sitosterols

To study the chemical constituents from the petroleum ether fraction of Buxus microphylla.. The petroleum ether fraction of Buxus microphylla was isolated and identified by silica gel column chromatography. And the anticancer activity of different chemical constituents was measured by MTT reduction test.. Eight compounds were isolated and identified as lupeol (1), butulin (3), beta-sitosterol (4), stigmasterol (5), dibutyl phthalate (6), 3beta, 30-dihydroxy-lup-20 (29) ene (7), daucosterol (8). Compound 7 inhibited KB cells' proliferation in a dose-dependent manner.. Compounds 2 - 5, 7, 8 are isolated from this genus for the first time. Compound 7 has certainly anticancer effects.

Topics: Buxus; Chromatography, Thin Layer; Dibutyl Phthalate; Ethers; Magnetic Resonance Spectroscopy; Molecular Structure; Pentacyclic Triterpenes; Plants, Medicinal; Sitosterols; Stigmasterol

To study the chemical constituents from Clematis brevicaudata.. The compounds were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR).. Eight compounds were isolated and identified as: palmitic acid (1), 1-docosanol (2), pentacosanoic acid-2', 3'-dihydroxypropyl ester (3), beta-sitosterol (4), daucosterol (5), a mixture of the trans-p-coumarate of the n-alkanols (6), 3,4-dihydroxy-trans coumatate ethyl ester (7), syringaresinol-O-D-glucopyranoside (8).. All these compounds are obtained from Clematis brevicaudata for the first time.

Topics: Clematis; Drugs, Chinese Herbal; Glucosides; Lignans; Magnetic Resonance Spectroscopy; Palmitic Acid; Plants, Medicinal; Sitosterols

To study chemical constituents of Changium smyrnioides Wolff.. The chemical components were isolated and purified by silica gel column and recrystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data.. Ten compounds were isolated and identified as lignoceric acid (1), beta-sitosterol (2), stigmasterol (3), 5-hydroxy-8-methoxypsoralen (4), glycerylmonopalmitate (5), L-pyroglutamic acid (6), succinic acid (7), vanillic acid-4-O-beta-D-glucopyranoside (8 ), vanillic acid (9), daucosterol (10).. Compounds 1, 4, 5, 6, 8 and 9 are obtained from the plant for the first time.

Topics: Apiaceae; Fatty Acids; Hydrolyzable Tannins; Plant Roots; Plants, Medicinal; Pyrrolidonecarboxylic Acid; Sitosterols; Stigmasterol; Succinic Acid; Vanillic Acid

To study chemical compounds from Pinus koraiensis.. The constituents were isolated by chromatographic method and the structures were identified on the basis of spectral alanlysis.. Eight compounds were identified as 8 (14)-podocarpen-13-on-18-oic acid (1), 15-hydroxydehydroabietic acid (2), 12-hydroxyabietic acid (3), lambertianic acid (4), dehydroabietic acid (5), sandaracopimaric acid (6), beta-sitosterol (7), daucosterol (8).. Compounds 1--6 are isolated from this plant for the first time.

Topics: Abietanes; Carboxylic Acids; Diterpenes; Fruit; Molecular Structure; Naphthalenes; Pinus; Plants, Medicinal; Sitosterols

To study the chemical constituents from the seeds of Allium cepa L., the constituents of the seeds of Allium cepa L.. To isolate and purify by silica gel, macroporous resin HP-20, Sephadex LH-20, RP-18 column.. Seven compounds were isolated from the EtOH extract of the seeds of Allium cepa., their structures were elucidated by physico-chemical properties and spectroscopic analysis as tianshic acid (I), N-trans-feruloyl tyramine (II), beta-sitosterol-3 beta-glucopyranoside-6'-palmitate (III), sitosterol (IV), daucosterol (V), tryptophane (VI), adenine riboside (VI).. Compounds V-VIII are obtained from this plant for the first time, compounds I-IV are isolated from the genus Allium for the first time.

Topics: Adenosine; Ethanol; Onions; Seeds; Sitosterols; Tryptophan

To study the chemical constituents of Rhizoma Cyperi.. The constituents were separated and purified by silica gel column chromatography, their structures were identified on the basis of physico-chemical properties and spectral data.. Six compounds were isolated and identified as physicion (1), hexadecanoic acid (2), beta-sitosterol (3), stigmasterol (4), catenarin (5), daucosterol (6).. Compounds 1, 4, 5 were isolated from this plant for the first fime.

Topics: Cyperus; Emodin; Magnetic Resonance Spectroscopy; Palmitic Acid; Plants, Medicinal; Rhizome; Sitosterols; Spectrophotometry, Ultraviolet; Stigmasterol

To study the chemical constituents of leaves of Paulownia fortunei (Seem.) Hemsl.. The constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis.. The compounds were identified as mimulone (I), apigenin (II), luteolin (III), 2alpha, 3beta, 19beta-trihydroxyurs-28-O-beta-D-galactonopyranos ylester (anserinoside, IV), 3alpha-hydroxyl-ursolicacid (V), ursolicacid (VI), daucosterol (VII), beta-sitosterol (VIII).. The compounds I - V are obtained from leaves of Paulownia fortunei (Seem.) Hemsl for the first time.

Topics: Apigenin; China; Luteolin; Magnetic Resonance Spectroscopy; Plant Leaves; Plants, Medicinal; Sapogenins; Scrophulariaceae; Sitosterols

To investigate the chemical constituents of the aerial parts of Ammopiptanthus mongolicus.. The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.. Ten compounds were isolated and identified as m-hydroxybenzoic acid (1), 1-(4-hydroxyphenyl) ethanone (2), beta-sitosterol (3), (-)-syringaresinol (4), (+)-lariciresinol (5), blumenol A (6), blumenol B (7), beta-daucosterol (8), coniferin (9), syringin (10).. The ten compounds were obtained from the genus Ammopiptanthus for the first time.

Topics: Cinnamates; Cyclohexanones; Drugs, Chinese Herbal; Fabaceae; Glucosides; Magnetic Resonance Spectroscopy; Phenylpropionates; Plant Components, Aerial; Sitosterols

To study the chemical constituents of Botrychium lanuginosum.. Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated by chemical evidence and spectroscopic methods.. Ten compounds were isolated from the 95% ethanol extract of the herb of B. lanuginosum and their structures were elucidated as 30-nor-21beta-hopan-22-one (1), beta-sitosterol (2), luteolin (3), thunberginol A (4), apigenin (5), (6'-O-palmitoyl)-sitosterol-3-O-beta-D-glucoside (6), daucosterol (7), 1-O-beta-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-[(2R-hydroxy hexadecanoyl) amino]-4, 8-octadecadiene-1, 3-diol (8), luteolin-7-O-glucoside (9), sucrose (10).. Compounds 1-10 were isolated from this genus for the first time.

Topics: Apigenin; Chromatography; Drugs, Chinese Herbal; Ferns; Isocoumarins; Luteolin; Sitosterols; Sucrose

Two new and seven known withanolides along with beta-sitosterol, stigmasterol, beta-sitosterol glucoside, stigmasterol glucoside, alpha+beta glucose were isolated from the roots of Withania somnifera. Among the known compounds, Viscosa lactone B, stigmasterol, stigmasterol glucoside and alpha+beta glucose are being reported from the roots of W. somnifera for the first time. One of the new compounds contained the rare 16beta-acetoxy-17(20)-ene the other contained unusual 6alpha-hydroxy-5,7alpha-epoxy functional groups in the withasteroid skeleton. The structures were elucidated by spectroscopic methods and chemical transformations.

Topics: Glucosides; Molecular Structure; Plant Extracts; Plant Roots; Sitosterols; Stigmasterol; Withania; Withanolides

To investigate the anti-HIV constituents from the root of Mirabilis jalapa.. The compounds were isolated by column chromatography on silica gel, Sephadex LH - 20, MCI-gel CHP-20P and RP-18. The structure were identified by means of NMR and MS analyses (1H-NMR, 13C-NMR, MS).. Eleven compounds were isolated and identified as astragaloside II (1), astragaloside II (2), astragaloside IV (3), astragaloside VI (4), flazin (5), 4'-hydroxy-2, 3-dihydroflavone 7-beta-D-glucopyranoside (6), gingerglycolipid A (7), 3, 4-dihydroxybenzaldehyd (8), p-hydroxybenzaldehyde (9), beta-sitosterol (10) and daucosterol (11).. Compounds 1-9 were obtained from this genus for the first time.

Topics: Benzaldehydes; Carbolines; Chromatography, Gel; Furans; Galactolipids; Magnetic Resonance Spectroscopy; Mass Spectrometry; Mirabilis; Plant Roots; Sitosterols

To study the chemical constituents in the whole herb of Anoectochiluts roxburghii (Wall) Lindl.. The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, NMR and MS evidences.. Fourteen compounds were obtained and there into ten were identified as beta-sitosterol(2), ferulic acid(3), oleanolic acid(6), lanosterol(7), p-hydroxybenzaldehyde(8), 3-methoxyl-p-hydroxybenzaldehlde(9), daucosterol(10),3',4',7-trimethoxy-3, 5-dihydroxyflavone(11), isorhamnetin-3-O-beta-D-rutinoside(12) and rutin(13).. Compounds 6,7,9 and 11 from genera Anoeetochilus were isolated for the first time.

Topics: Coumaric Acids; Flavones; Lanosterol; Molecular Structure; Oleanolic Acid; Orchidaceae; Plants, Medicinal; Sitosterols; Spectrophotometry, Ultraviolet

To study the chemical constituents of Oxytropis chiliophylla.. The air-dried whole plants of O. chiliophylla were extracted with EtOH (90%) three times at room temperature. The compounds were isolated by silica-gel, polyamide, C-18 and Sphadex LH -20 columns. The structures were identified based on spectral analysis.. 8 compounds were isolated from O. chiliophylla and identified as azukisapogenol (1), (22E, 24R) -24-methyl-5alpha-cholesta-7, 22-diene-3beta, 5alpha, 6beta-triol (2), apigein (3), 3', 4'-dimethoxy-quercetin-3-O-beta-D-galactopyranoside (4), 7, 3', 4'-trimethoxy-quercetin-3-O-alpha-L-rhamopyranosyl-(1-->2)-beta-D-glucopyranoside (5), (2S, 3S, 4R)-N-[(R)-2'-hydroxytetracosanoyl]-1, 3, 4-trihydroxy-2-amino-octadeca-6-ene (6), beta-sitosterol (7), daucosterol (8).. All the compounds were isolated from O. chiliophylla for the first time.

Topics: Oxytropis; Plants, Medicinal; Sapogenins; Sitosterols

To study the chemical constituents of Gueldenstaeditia stenophylla.. The constituents were isolated by alcohol extraction, column chromatography on silica gel. Their structures were elucidated by chemical and spectroscopic methods.. Six compounds were obtained, and five of them were identified as n-hexadecanioc acid (I), beta-sitosterol (II), daucosterol (III), apigenin (IV), D-fructose (VI). Compound V was being determined.. Five compounds are isolated from Gueldenstaedtia stenophylla and compounds I, III are extracted from Gueldenstaedtia Fisch for the first time.

Topics: Apigenin; Fabaceae; Palmitic Acid; Plants, Medicinal; Sitosterols; Spectrophotometry, Ultraviolet

To study the chemical constituents of Heterosmilax yunianensis.. The compounds were isolated and repeatedly purified with chromatograph and the structures were elucidated by physico-chemical properties and spectral analysis.. Eight compounds were obtained and elucidated as beta-sitosterol (I), glycerol monopalmitate (II), daucosterol (IIl), hexacosanoic acid (IV), 5-hydroxymethyl furaldehyde (V), hergapen (VI), ursolic acid (VII), liquiritigenin (VIII).. They have been isolated from this plant for the first time, and IV - VIII are obtained from the plants of Heterosmilax for the first time.

Topics: Fatty Acids; Flavanones; Lactones; Molecular Structure; Plants, Medicinal; Rhizome; Sitosterols; Smilacaceae; Triterpenes; Ursolic Acid

Six compounds were isolated and purified from Orobanche coerulescens by extraction and different kinds of column chromatography. The structures were determined on the basis of spectral analysis. The structures were elucidated as D-mannitol(I), beta-sitosterol(II), succinic acid(III), caffeic acid(IV), protocatechuic aldehyde(V) and daucosterol(VI). All compounds are obtained from this plant for the first time.

Topics: Benzaldehydes; Caffeic Acids; Catechols; Chromatography, Thin Layer; Ethanol; Mannitol; Orobanche; Plants, Medicinal; Rhizome; Sitosterols; Succinic Acid

To study the chemical constituents of Taxillus sutchuenenisis (Lecomte) Danser.. Chromatography and spectrum analysis were employed to isolated and elucidate the chemical constituents in the plant.. 9 compounds were isolated and identified as quercetin (I), quervetin 3-O-beta-D-galactoside (II), isoquercitrin (III), quercitrin (IV), rutin (V), gallic acid (VI), ferulic acid (VII), beta-sitosterol (VIII), daucosterol (IX), respectively.. Compounds III-IX are isolated from this plant for the first time. The work provide evidence for the exploitation and utilization of this plant resouce.

Topics: Coumaric Acids; Gallic Acid; Loranthaceae; Molecular Structure; Plants, Medicinal; Quercetin; Rhizome; Rutin; Sitosterols; Spectrophotometry, Ultraviolet

To investigate the chemical constituents from aerial part of Curcuma wenyujin.. Compounds were isolated by repeated column chromatography on silica gel. Their structures were elucidated on the basis of spectral analysis and comparison with literature data.. Six compounds were isolated and identified as codonolactone (1), voleneol (2), octacosanoic acid (3), beta-sitosterol (4), mangdesisterol (5), and daucosterol (6).. Compounds 1, 2, and 5 were isolated from the plant for the first time.

Topics: Curcuma; Fatty Acids; Phytosterols; Plant Components, Aerial; Plants, Medicinal; Sesquiterpenes; Sitosterols

To study the chemical constituents of Bauhinia championii.. Compounds were isolated from the ethanolic extract of B. championii by silica gel column Chromatography, and their structures were elucidated by spectral analyses.. Five compounds were isolated and elucidated as 2,4,6-trimethoxyphenol 1-O-beta-D-(6'-O-galloyl)-glucopyranoside (1), (+/-)-lyoniresinol (2), daucosterol (3), beta-sitosterol (4) and gallic acid (5).. Compounds 1-4 were isolated from B. championii for the first time.

Topics: Anisoles; Bauhinia; Gallic Acid; Glucosides; Molecular Structure; Naphthalenes; Plant Stems; Plants, Medicinal; Sitosterols

To study the chemical constituents of the 95% ethanol extract from the rhizome of Smilax china.. Seven compounds were isolated from ethyl acetate fraction of 95% ethanol extract by using repeated silica column chromatography, partition chromatography, preparing TLC, and other methods. Structure of three compounds of them was identified based on chemical methods and spectroscopic methods.. The structure of them were established as dihydrokaempferol-5-O-beta-D-glucoside (I), beta-sitosterol (II), daucosterol (III).. Compound I was a novel compound which was first found from natural plants.

Topics: Flavonoids; Glucosides; Molecular Structure; Plants, Medicinal; Rhizome; Sitosterols; Smilax; Spectrophotometry, Ultraviolet

To study the chemical constituents from rhizome of Pulsatilla dahurica.. The constituents were isolated and purified by various chromatographic methods. AR compounds were identified on the basis of spectral analysis and physico-chemical characters.. Six compounds were isolated from the 70% alcohol extract of the rhizome identified as hederagenin ( I ), hederagenin 3-O-alpha-L-arabinopyranoside (II), hederagenin 3-O-beta-D-glucopyranosyl(1-->2)-alpha-L-arabinopyranoside (III), hederagenin 3-O-beta-D-glucopyranosyl(1 -->2) [beta-D-glucopyranosyl(1-->4)]-alpha-L-arabinopyranoside (IV), beta-sitosterol (V) and daucosterol (VI), respectively.. Compounds I approximately VI were isolated from this plant for the first time.

Topics: Oleanolic Acid; Plants, Medicinal; Pulsatilla; Rhizome; Saponins; Sitosterols

An ethyl acetate extract of Oryza sativa (rice) hulls yielded seven compounds: hentriacontane, 1-tetratriacontanol, beta-sitosterol, momilactone A, momilactone B, tricin (a flavonoid), and beta-sitosterol-3-O-beta-D-glucoside. The structures of these compounds were elucidated with 500 MHz nuclear magnetic resonance (NMR), using 1D and 2D spectral methods, aided by electron ionization mass spectrometry (EI-MS), fast atom bombardment mass spectrometry (FAB-MS), infrared (IR), and ultraviolet (UV) spectrophotometry. The complete 1H NMR assignments for momilactone A and B and 13C NMR assignments for tricin are discussed. To the best of our knowledge, hentriacontane, 1-tetratriacontanol, and beta-sitosterol-3-O-beta-D-glucoside were identified for the first time in rice hulls. In biological activity tests using these identified compounds, momilactone A and B showed potent inhibitory activity against duckweed (Lemna paucicostata). 1-Tetratriacontanol and beta-sitosterol-3-O-beta-D-glucoside also showed about 13-20% inhibitory activity based on chlorophyll reduction. Hentriacontane and beta-sitosterol did not show any herbicidal activity. In a germination assay of three weed species (Leptochloa chinenesis L., Amaranthus retroflexus L., and Cyperus difformis L.) in culture tubes both momilactones A and B had high inhibitory effects. Momilactone B completely inhibited germination of all three weed species at 20 ppm. Germination of L. chinensis L. was completely inhibited by a 4 ppm solution of momilactone B.

Topics: Amaranthus; Cyperus; Diterpenes; Fatty Alcohols; Flavonoids; Germination; Herbicides; Hydrocarbons; Lactones; Oryza; Plant Development; Plants; Sitosterols; Spectrum Analysis

We hypothesized that the phytosterols beta-sitosterol (BSS), beta-sitosterol glucoside (BSSG), and Moducare (MC; BSS:BSSG = 99:1) could modulate the growth of estrogen-dependent human breast cancer cells in vitro and in vivo. The present study evaluated the estrogenic and antiestrogenic effects of BSS, BSSG, and MC (0.001 to 150 micromol/L) on the proliferation of Michigan Cancer Foundation 7 (MCF-7) cells in vitro. Both BSS (>1 micromol/L) and MC (>50 micromol/L) increased MCF-7 cell proliferation. Treatment with 150 micro mol/L of BSS and MC increased cell growth by 2.4 and 1.5 times, respectively, compared to the negative control (NC) group. However, BSSG had no effect at the concentrations tested. The effects of dietary BSS, BSSG, and MC on the growth of MCF-7 cells implanted in ovariectomized athymic mice were also evaluated. Estrogenic effects of the phytosterols were evaluated in the NC, BSS, BSSG, and MC treatment groups, and antiestrogenic effects were evaluated in the 17 beta-estradiol (E(2)), E(2) + BSS, E(2) + BSSG, and E(2) + MC treatment groups. Mice were treated with dietary BSS (9.8 g/kg AIN93G diet), BSSG (0.2 g/kg diet), or MC (10.0 g/kg diet) for 11 wk. Dietary BSS, BSSG, and MC did not stimulate MCF-7 tumor growth. However, dietary BSS, BSSG, and MC reduced E(2)-induced MCF-7 tumor growth by 38.9% (P < 0.05), 31.6% (P = 0.08), and 42.13% (P < 0.05), respectively. The dietary phytosterols lowered serum E(2) levels by 35.1, 30.2, and 36.5% in the E(2) + BSS, E(2) + BSSG, and E(2) + MC groups, respectively (P < 0.05), compared to that of the E(2) treatment group. Estrogen-responsive pS2 mRNA expression in tumors did not differ among groups, but expression of the antiapoptotic marker B-cell lymphoma/leukemia-2 (bcl-2) in tumors from the E(2) + MC group was downregulated, compared to that of the E(2) treatment group. In summary, BSS and MC stimulated MCF-7 cell growth in vitro. Although BSSG comprises only 1% of MC, BSSG made MC less estrogenic than BSS alone in vitro. However, dietary BSS and MC protected against E(2)-stimulated MCF-7 tumor growth and lowered circulating E(2) levels.

Topics: Animals; Breast Neoplasms; Cell Division; Cell Line, Tumor; Drug Combinations; Drug Implants; Estradiol; Estrogen Antagonists; Female; Humans; Mammary Neoplasms, Experimental; Membrane Proteins; Mice; Mice, Nude; Neoplasm Transplantation; Organ Size; Ovariectomy; Phytosterols; Presenilin-2; Proto-Oncogene Proteins c-bcl-2; Sitosterols; Uterus

To study the major chemical constituents from the root tuber of Typhyonium flagelliforme.. Compounds were separated and purified with silica gel column and preparative HPLC, and their structures were elucidated on the basis of spectroscopic evidence (UV, IR, MS, NMR and 2D NMR).. Four compounds were identified as 1-O-beta-glucopyranosyl-2-[(2-hydroxyloctadecanoyl) amido]-4,8-octadecadiene-1,3-diol (1), coniferin (2), beta-sitosterol (3) and beta-daucosterol (4).. A cerebroside with significant antihepatotoxic activity and a phenylpropanoid glycoside were isolated from Typhyonium flagelliforme for the first time.

Topics: Araceae; Cinnamates; Glucosides; Molecular Conformation; Molecular Structure; Plant Roots; Plants, Medicinal; Sitosterols

To study the chemical constituents from the herb of Gentianopsis paludosa.. Column chromatogrophy and spectral analysis were used to isolate and identify the constituents.. Six compounds were isolated and identified as 1,7-dihydroxy-3,8-dimethoxyxanthone (I), 1-hydroxy-3, 7, 8-trimethoxyxanthone (II), 1, 8-dihydroxy-3, 7-dimethoxyxanthone (III), 1-hydroxy-3, 7-dimethoxyxanthone (IV), beta-sitosterol (V), daucosterol (VI).. Compounds III-VI were isolated from the plant for the first time.

Topics: Gentianaceae; Plants, Medicinal; Sitosterols; Xanthones

To study the chemical constituents from Lamium maculatum L. var Kansuense.. The chemical constituents were isolated and repeatedly purified by silica gel column chromatography and the structures were elucidated by the NMR spectra and physico-chemical properties.. Ten compounds were obtained and they were identified as D-mannitol, beta-sitosterol, stigmasterol, rutin, 3'-methylquercetin-3-O-rutinoside, n-butyl-beta-D-fructopyranoside, daucosterol, acteoside, 20-hydroxyecdysone, allantoin.. All the compounds were obtained from L. maculatum L. var Kansuense for the first time.

Topics: Allantoin; Ecdysterone; Glucosides; Lamiaceae; Mannitol; Phenols; Plants, Medicinal; Rutin; Sitosterols; Stigmasterol

One sphingolipid, 1-O-beta-D-glucopyranosyl-(2S,3R,4E,8Z)-2-[(2R)-2-hydroxyhexadecanoylamino]-4,8-octadecadiene-1,3-diol, and four other constituents, beta-sitosterol, daucosterol, uridine, and adenosine, have been isolated from the nuts of almond (Prunus amygdalus). Complete assignments of the proton and carbon chemical shifts for the sphingolipid were accomplished on the basis of high-resolution 1D and 2D NMR data. All of these compounds are being reported from almond nuts (P. amygdalus)for the first time.

Topics: Adenosine; Chromatography; Magnetic Resonance Spectroscopy; Nuts; Prunus; Sitosterols; Sphingolipids; Uridine

Beta-sitosterol and beta-sitosteryl-beta-D-glucoside were isolated as analgesic constituents from the leaves of Mentha cordifolia Opiz. The acetic acid-induced writhing test showed that beta-sitosterol and beta-sitosteryl-beta-D-glucoside decreased the number of squirms induced by acetic acid by 70.0% and 73.0%, respectively, at a dose of 100 mg / kg mouse. Statistical analysis using the Kruskall Wallis one-way analysis of variance by ranks showed that these isolates approximate the analgesic activity of mefenamic acid at a 0.001 level of significance. The hot plate method confirmed their analgesic activities, as beta-sitosterol and beta-sitosteryl-beta-D-glucoside exhibited a 300% and 157% increase in pain tolerance, respectively, while mefenamic acid, a known analgesic, showed a 171% increase. Neither isolate exhibited antiinflammatory activity using the carrageenan-induced mouse paw oedema assay. Beta-sitosterol also exhibited anthelminthic and antimutagenic activities. In vitro tests using live Ascaris suum as test animals showed that the behaviour of worms treated with beta-sitosterol approximated that of the positive controls, Combantrin and Antiox. An in vivo micronucleus test showed that beta-sitosterol inhibited the mutagenicity of tetracycline by 65.3% at a dose of 0.5 mg /kg mouse. At the same dose, it did not exhibit chromosome-breaking activity.

Topics: Acetic Acid; Analgesics; Animals; Anthelmintics; Anti-Inflammatory Agents; Ascaris suum; Drug Evaluation, Preclinical; Edema; Extremities; Gas Chromatography-Mass Spectrometry; Hot Temperature; Mefenamic Acid; Mentha; Mice; Micronucleus Tests; Pain; Pain Threshold; Plant Extracts; Plant Leaves; Plants, Medicinal; Sitosterols

To study the chemical constituents from Salvia roborowskii Maxim.. Chromatography and spectroscopic analysis were employed to isolate and elucidate the chemical constituents in the plant.. Six compounds were isolated and elucidated as lupeol (I), 11 beta-hydroxy-lupeol(II),3 beta-acetyl-11 beta-hydroxy-lupeol(III), ursolic acid (IV), beta-sitosterol(V), daucosterol (VI).. I approximately VI were isolated from this plant for the first time.

Topics: Chromatography, Thin Layer; Molecular Structure; Pentacyclic Triterpenes; Plants, Medicinal; Salvia; Sitosterols; Triterpenes; Ursolic Acid

Topics: Arabidopsis Proteins; Binding Sites; Carbohydrate Conformation; Catalysis; Cell Membrane; Cellulase; Cellulose; Dextrins; Glucans; Glucose; Glucosyltransferases; Gossypium; Membrane Proteins; Models, Biological; Organisms, Genetically Modified; Plants; Sitosterols; Uridine Diphosphate Glucose; Yeasts

Cellulose synthesis in plants requires beta-1,4-glucan chain initiation, elongation, and termination. The process of chain elongation is likely to be distinct from the process of chain initiation. We demonstrate that a CesA glucosyltransferase initiates glucan polymerization by using sitosterol-beta-glucoside (SG) as primer. Cotton fiber membranes synthesize sitosterol-cellodextrins (SCDs) from SG and uridine 5'-diphosphate-glucose (UDP-Glc) under conditions that also favor cellulose synthesis. The cellulase encoded by the Korrigan (Kor) gene, required for cellulose synthesis in plants, may function to cleave SG from the growing polymer chain.

Topics: Arabidopsis Proteins; Calcium; Cell Wall; Cellobiose; Cellulase; Cellulose; Dextrins; Egtazic Acid; Glucans; Glucose; Glucosyltransferases; Gossypium; Herbicides; Membrane Proteins; Nitriles; Recombinant Proteins; Sitosterols; Uridine Diphosphate Glucose; Yeasts

To study the lipid chemical constituents of Salvia sochifolia growing in Yunnan province.. Chromatographic techniques were used to isolate the compounds and the structures were identified by spectral analysis.. Five compounds were isolated from the plant and identified as 2 alpha, 3 alpha-dihydroxyurs-12-en-28-oic acid, 2 alpha, 3 alpha, 24-trihydroxyurs-12-en-28-oic acid, ursolic acid, daucosterol and beta-sitosterol.. These compounds were isolated from the plant for the first time.

Topics: Plant Roots; Plants, Medicinal; Salvia; Sitosterols; Triterpenes; Ursolic Acid

Eicosane acid, beta-sitosterol, oleanolic acid, ursolic acid and daucosterol have been firstly isolated and identified from Schizonepeta tenuifolia Briq..

Topics: Eicosanoic Acids; Lamiaceae; Oleanolic Acid; Sitosterols; Triterpenes; Ursolic Acid

The chemical constituents of the herb of Cuscuta chinensis Lam. were investigated. Five compounds were isolated from petroleum ether and chloroform fraction. Their structures were identified as beta-sitosterol, d-sesamin, 9(R)-hydroxy-d-sesamin, d-pinoresinol and daucosterol by chemical and spectroscopical methods. All these compounds were isolated from the stem for the first time.

Topics: Chemical Fractionation; Cuscuta; Dioxoles; Drugs, Chinese Herbal; Furans; Lignans; Sitosterols; Spectrum Analysis

Study on the non-anthraquinone constituents from rhizoma and radix of Rheum sublanceolatum.. The constituents were isolated through column chromatography and identified on the basis of their physiochemical and spectral data.. Six non-anthraquinone constituents were isolated and identified as n-octacosanic acid, sitosterol, daucosterol, 2-methyl-5-carboxymethyl-7-hydroxychromone, piceatannol and 6-hydroxymusizin-8-O-beta-D-glucopyranoside.. All these compounds were firstly isolated from R. sublanceolatum.

Topics: Plant Roots; Plants, Medicinal; Rheum; Rhizome; Sitosterols; Stilbenes

Friedelin, epifriedelinol, beta-amyrin, beta-sitosterol, beta-sitosterol 3-beta-D-glucopyranoside, and naringin were isolated from the dried rhizome of Drynaria quercifolia. The methanol extract showed broad and concentration-dependent antibacterial activity.

Topics: Anti-Bacterial Agents; Anti-Infective Agents; Bacteria; Candida albicans; Dose-Response Relationship, Drug; Flavanones; Flavonoids; Humans; Microbial Sensitivity Tests; Oleanolic Acid; Phytotherapy; Plant Extracts; Plant Roots; Plants, Medicinal; Sitosterols; Triterpenes

To investigate the chemical constituents from the herb Eremurus chinensis.. The chemical constituents were extracted with solvent and separated with column chromatography.. The structures were identified as chrysophanol 8-Me ether, aloesaponol III, daucosterol and beta-sitosterol.. These compounds were separated from genus Eremurus first time.

Topics: Liliaceae; Plants, Medicinal; Sitosterols

To study the chemical constituents of Lagopsis supina.. Compounds were separated by solvent extraction, column chromatography and TLC, and the structures were determined by chemical and spectral analyses.. Six compounds were isolated from the ethanol extract of L. supina and identified as eicosanoic acid octadecyl ester, eicosanoic acid-16-methyl-15, 16-hetadecenyl ester, palmitic acid, beta-sitosterol, oleanolic acid and daucosterol.. Except beta-sitosterol, all the compounds were isolated from this genus for the first time.

Topics: Lamiaceae; Oleanolic Acid; Palmitic Acid; Plants, Medicinal; Sitosterols

From the butanolic extract of petals of Lilium candidum L., beta-sitosterol and beta-sitosterol glucoside were isolated. The isolated compounds were identified by spectroscopic means and by comparison with literature data; beta-sitosterol glucoside was isolated in genus Lilium L. for the first time.

Topics: Plant Extracts; Sitosterols

The free radical scavenging abilities of the structurally related steroids beta-sitosterol, beta-sitosterol glucoside (plant sterols and sterolins), cholesterol, and dehydroepiandrosterone sulphate (DHEAS) were compared with melatonin (an efficient free radical scavenger) in an in vitro system which measures lipid peroxidation of platelet membranes in the presence of iron (Fe2+). Lipid peroxidation is a process whereby cellular membranes are damaged due to the oxidative deterioration of polyunsaturated lipids, which may lead to cell death and disease in living organisms. Substances such as vitamin E protect cellular membranes against oxidative damage due to their chemical structures. The steroids cholesterol, beta-sitosterol, beta-sitosterol glucoside and dehydroepiandrosterone (DHEA) are structurally related to each other. During aging, serum concentrations of DHEA, DHEAS and melatonin decrease, while the concentration of cholesterol tends to increase. The aim of the present study was to compare the role these substances play in lipid peroxidation over a wide concentration range. At concentrations lower than the free iron in the reaction mixture, all the steroids investigated decreased lipid peroxidation. At higher concentrations, cholesterol and beta-sitosterol increased lipid peroxidation, while DHEAS and melatonin continued to decrease lipid peroxidation.

Topics: Aging; Androsterone; Antioxidants; Blood Platelets; Cholesterol; Free Radical Scavengers; Humans; Hypolipidemic Agents; In Vitro Techniques; Lipid Peroxidation; Melatonin; Sitosterols

To separate compounds from the root of Gypsophila oldhamiana.. Chemical and chromatographic methods were used to separate the compounds. IR, MS, NMR were used to determine the structures of compounds.. Compound pentacosanoic acid, lacceroic acid, beta-sitosterol, alpha-spinasterol, daucosterol and sucrose were identified.. All the above compounds were obtained from this genus for the first time.

Topics: Caryophyllaceae; Plant Roots; Plants, Medicinal; Sitosterols; Stigmasterol

Seven compounds were isolated from Quercus mongolica Fisch., six (1-VI) of them were identified as fridelin (I), beta-amyrin (II), 1-octadecanol (III), beta-sitosterol (IV), daucosterol (V) and gallic acid (VI) by means of chemical, physical and spectrosopic evidiences.

Topics: Fatty Alcohols; Gallic Acid; Oleanolic Acid; Plant Leaves; Plants, Medicinal; Quercus; Sitosterols; Spectrophotometry, Ultraviolet; Triterpenes

To investigate the chemical constituents of Tetrastigma hemsleyanum.. Compounds were separated by rechromatography on silica gel from the chloroform solubles of its ethanol extracts, and the structures were determined by spectral analysis and chemical evidence.. Three compounds were isolated and elucidated as 6'-O-benzoyldaucosterol, daucosterol and beta-sitosterol.. All the three compounds were isolated from this plant for the first time, of which 6'-O-benzoyldaucosterol is a new natural product.

Topics: Drugs, Chinese Herbal; Magnoliopsida; Molecular Structure; Plants, Medicinal; Sitosterols

Lilium brownii F. E. Brown var. viridulum Baker is a well-known and very important traditional Chinese medicine. It has been used as sedative, antitussive, anti-inflammatory or as a nutrient. Five compounds were isolated from the bulbs of Lilium brownii. On the basis of spectroscopic analysis (IR, FAB-MS, 1HNMR, 13CNMR, DEPT, HMQC, HMBC) and their physicochemical properties, they were identified as beta-sitosterol (I), daucosterol (II), n-butyl-beta-D-fructopyranoside (III), 26-O-beta-D-glucopyranosyl-3 beta, 26-dihydroxy-5-cholesten-16, 22-dioxo-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside (IV), 26-O-beta-D-glucopyranosyl-3beta, 26-dihydroxy cholestan-16, 22-dioxo-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside(V). Among them, I, II and III were isolated from this plant for the first time, IV and V are new compounds.

Topics: Drugs, Chinese Herbal; Lilium; Molecular Structure; Saponins; Sitosterols

Three compounds were isolated from the root of Boehmeria nivea and elucidated according to their spectroscopic data as beta-sitosterol, daucosterol and 19 alpha-hydroxyursolic acid. They are obtained from the herb for the first time.

Topics: Drugs, Chinese Herbal; Magnoliopsida; Plant Roots; Plants, Medicinal; Sitosterols; Triterpenes

Three compounds were obtained for the first time from the rhizome of Sparganium stoloniferum and identified as beta-sitosterol, succinic acid and daucosterol by comparing with the known specimens. Twenty-one fatty acids were separated and identified by GC-MS.

Topics: Drugs, Chinese Herbal; Fatty Acids; Sitosterols

Topics: Drugs, Chinese Herbal; Mannitol; Sitosterols

Four compounds were isolated from the fruit of cultivated Panax ginseng and identified as beta-sitosterol,20(R)-protopanaxatriol, daucosterine and 20(R)-ginsenoside-Rg3 on the basis of physicochemical constants and spectral evidences.

Topics: Fruit; Panax; Plants, Medicinal; Sapogenins; Sitosterols; Triterpenes

Topics: Drugs, Chinese Herbal; Glucosides; Phenylpropionates; Sitosterols

Beta-sitosterol-beta-D-glucoside and its aglycone (the major constituent of the seed oil of Hippophae rhamnoides L.) were investigated for their antigastroulcerative activity in rats. Two experimental gastric ulcer models were selected: chronic acetic acid-induced ulcers and cold stress-induced ulcers. Both the glucoside and its aglycone showed antiulcerative activity in chronic acetic acid-induced gastric ulcer models, and their effects were at least comparable to the effects of wishupin in combination with cimetidine. The effect of aglycone appears better than the glucoside's. Glucoside also showed visibly antiulcerative effects on cold stress-induced ulcers, but wishupin combined with cimetidine did not have such effects.

Topics: Acetates; Acetic Acid; Animals; Anti-Ulcer Agents; Female; Male; Rats; Rats, Inbred Strains; Sitosterols; Stomach Ulcer; Stress, Physiological

From the extract of dry roots of Rehmannia glutimosa, fatty acids, palmitic acid, beta-sitosterol, daucosterol and sulfur (Ss) cyclic compound were isolated and identified. The latter four were isolated for the first time.

Topics: Drugs, Chinese Herbal; Fatty Acids; Palmitic Acids; Sitosterols

beta-Sitosterol, beta-sitosterol-beta-D-glucopyranoside and a butyrolactone lignan disaccharide, ramontoside, were isolated from the heartwood of Flacourtia ramontchi. The structure of ramontoside was determined as diphyllin-4-O-[beta-D-glucopyranosyl(1---4)]-beta- 2,3-di-O-methyl-D-xylopyranoside by hydrolysis and spectral data.

Topics: Acetylation; Carbohydrate Sequence; Dioxolanes; Glycosides; Hydrolysis; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Molecular Structure; Plants, Medicinal; Sitosterols

The bulb of Fritillaria anhuiensis, a substitute for the traditional Chinese medicine Beimu, has been used as antibechic and expectorant. From the either soluble part of the bulb, four constituents were isolated and identified by spectral analysis (IR, MS, 1HNMR, 13CNMR) as beta-sitosterol, daucosterol, ent-kaur-15-en-17-ol and ent-kauran-16 beta, 17-diol.

Topics: Diterpenes; Drugs, Chinese Herbal; Sitosterols

Four compounds were isolated from the roots of Adenophora stricta. On the basis of spectrometric analysis and physicochemical constants, they were identified as beta-sitosterol, beta-sitosterol-O-beta-D-glucopyranoside, taraxerone and octacasanoic acid respectively. Taraxerone and octacasanoic acid were isolated from the plants of Adenophora for the first time.

Topics: Drugs, Chinese Herbal; Fatty Acids; Oleanolic Acid; Sitosterols

Heptatriacontane, benzoic acid, succinic acid, palmitic acid, stearic acid, beta-sitosterol, stigmasterol, daucosterol, D-mannitol, D-glucose, sucrose and choline chloride were isolated from the roots of Arisaema fargesii collected from Gansu Province. Their structures were elucidated by spectroscopic methods and a few chemical transformations. The twelve compounds have not been reported before as constituents of Arisaema fargesii.

Topics: Antineoplastic Agents, Phytogenic; Drugs, Chinese Herbal; Sitosterols; Succinates; Succinic Acid

In this paper four compounds isolated from Radix Ophiopogonis are described. Their structures have been identified as beta-sitosterol (I), 6-aldehydo-7-methoxy-isoophiopogonanone B (II), beta-sitosterol-beta-D-glucopyranoside (III) and 1-borneol-beta-D-apisyl-beta-D-glucopyranoside (IV) on the basis of spectral data and hydrolytic reaction.

Topics: Camphanes; Drugs, Chinese Herbal; Glucosides; Isoflavones; Saponins; Sitosterols

Seven compounds were isolated from the roots of Aralia continentalis. Four of these compounds were identified as ent-kaur-16-en-19-oic acid, beta-sitosterol glucoside (daucosterol), beta-sitosterol and 16 alpha-hydroxy-(-)-kauran-19-oic acid. It is the first time that 16 alpha-hydroxy-(-)-karan-19-oic acid and beta-sitosterol glucoside were isolated from this plant.

Topics: Diterpenes; Drugs, Chinese Herbal; Sitosterols