gamma-sitosterol and luteolin-7-glucoside

To study the chemical constituents from Hibiscus syriacus leaves and their α-glucosidase inhibitory activities.. Column chromatography including macroporous resins, silica gel and Sephadex LH-20 were used for the isolation and purification of all compounds. Spectroscopic methods including physical and chemical properties, 1H-NMR and 13C-NMR were used for the identification of structures. Their α-glucosidase inhibitory activities were detected by a 96-well microplate.. 15 compounds were isolated and identified as β-sitosterol(1), β-daucostero (2), β-amyrin (3), oleanolic acid (4), stigmast-4-en-3-one (5), friedelin (6), syriacusin A (7), kaempferol (8), isovitexin (9), vitexin (10), apigenin (11), apigenin-7-O-β-D-glucopyranoside (12), luteolin-7-O-β-D-glucopyranoside (13), vitexin-7-O-β-D-glucopyranoside (14) and rutin (15).. All the compounds are isolated from the leaves of Hibiscus syriacus for the first time. Taking acarbose as positive control, the α-glucosidase inhibitory activities of 15 compounds were evaluated. Compounds 7 and 9 have shown strong α-glucosidase inhibitory activities with IC50 of 39.03 ± 0.38 and 32.12 ± 0.62 mg/L, inhibition ratio of 94.95% and 97.15%, respectively.

Topics: alpha-Glucosidases; Apigenin; Glucosides; Glycoside Hydrolase Inhibitors; Hibiscus; Kaempferols; Luteolin; Oleanolic Acid; Phytochemicals; Plant Leaves; Sitosterols; Stigmasterol; Triterpenes

To study the chemical constituents of aerial parts of Paris polyphylla var. chinensis .. Aerial parts of Paris polyphylla var. chinensis was extracted with 95% EtOH, and separated and purified by silica gel, RP 18 and Sephadex LH-20 col- umn chromatography. The structures were identified by spectroscopic analysis.. A total of ten compounds were isolated and iden- tified as β-sitosterol (1) ergosta-7, 22-dien-3-one (2), β-ecdysone (3), kaempferol (4), daucosterol (5) luteolin (6) calonysterone (7), luteolin-7-O-glucoside (8), quercetin (9), and 3β, 5α, 9α-trihydroxyergosta-7, 22-dien-6-one (10).. Compounds 2,6 and 10 are isolated from Paris polyphylla var. chinensis for the first time.

Topics: Ecdysterone; Flavones; Glucosides; Kaempferols; Liliaceae; Phytochemicals; Plant Components, Aerial; Plant Extracts; Plants, Medicinal; Quercetin; Sitosterols

To study the chemical constituents of the leaf of Salix matsudana.. The chemical constituents were isolated by column chromatography on silica gel, Sephadex LH-20 and MPLC. Their structures were elucidated on the basis of spectral analysis.. Eight compounds were isolated and their structures were identified as beta-Sitosterol(1),5,7-Dihydroxychromone-7-O-beta-D-glucopyranoside(2), (2S)-Helichrysin A(3), (2R)-Helichrysin A(4), Luteolin-7-O-beta-D-glucoside(5), Salicin(6), Apigenin-7-O-beta-D-glucopyranside(7), Lutelion-3'-methylether-7-O-f-D-glucopyranside(8).. Compounds 1-4 are isolated from this plant for the first time.

Topics: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Glucosides; Luteolin; Molecular Structure; Plant Leaves; Salix; Sitosterols

To study the chemical constituents from the antioxidant fraction of Neo-Taraxacum siphonathum.. Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated on the basis of chemical evidence and spectral analysis.. Ten compounds were isolated and identified from Neo-T. siphonathum, caffeic acid (1), chlorogenic acid (2), quercetin (3), luteolin (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin-3-O-alpha-D-arabinofuranoside (6), quercetin-3-O-alpha-D-arabinopyranoside (7), luteolin-7-O-beta-D-glucopyranoside (8), beta-sitosterol (9) and daucosterol (10).. Compounds 1-10 were isolated from this plant for the first time.

Topics: Caffeic Acids; Chlorogenic Acid; Glucosides; Luteolin; Magnetic Resonance Spectroscopy; Quercetin; Sitosterols; Taraxacum

To study the chemical constituents from Aquilegia ecalcarata.. Compounds were isolated with silica gel and polyamide chromatography and their structures were determined by spectral analysis and chemical evidence.. Five compounds were obtained and identified as beta-sitosterol, isoorientin-7-O-glucoside, isovitexin-4'-O-glucoside, isovitexin-2'-O-rhamnoside, luteolin-7-O-glucoside.. All the compounds were isolated from this plant for the first time.

Topics: Aquilegia; Flavonoids; Glucosides; Isoflavones; Luteolin; Plants, Medicinal; Sitosterols

To study chemical constituents of the above ground part of Salix oritrepha.. Using chromatography to isolate compounds and their structure were identified by physical, chemical and spectral techniques.. Four compounds were isolated and elucidated as beta-sitosterol, beta-sitosteryl-3-O-glucoside, friedelin, luteolin-7-O-glucoside.. All of them were isolated from S. oritrepha for the first time. Friedelin from the plants of genus Salix for the first time.

Topics: Flavonoids; Glucosides; Luteolin; Plant Components, Aerial; Plants, Medicinal; Salix; Sitosterols; Tibet; Triterpenes

The paper deals with the isolation of constituents from light petrol and methanol extracts of the leaves of Holodiscus discolor (PURSH) MAXIM., Rosaceae. beta-sitosterol and taraxasterol were isolated from the light petrol extract, luteolin-7-O-glucoside was isolated from the methanol extract. The isolated compounds were identified by spectroscopic means and by comparison with authentic samples.

Topics: Flavonoids; Glucosides; Luteolin; Plant Leaves; Plants, Medicinal; Rosaceae; Sitosterols; Sterols; Triterpenes