gamma-sitosterol and hyperoside

To study the chemical consituents of Hypericum ascyron.. The constituents were isolated and purified by chromatography on silica gel; the structure of the compound was determined by MS and NMR spectral analysis.. On the basis of spectroscopic analysis and comparison with the reported data, they were identified as hyperoside( 1),hypercalin B( 2),hypercalin C( 3),1,7-dihydroxyxanthone( 4),2,3-dimethoxyxanthone( 5),1-hydroxy-7-methoxyxanthone( 6),rutin( 7),kaempferol( 8),toxyloxanthone B( 9),quercetin( 10),quercitrin( 11),β-daucosterol( 12) and β-sitosterol( 13).. Compounds 2,3,6 and 9 are obtained from this plant for the first time.

Topics: Acetates; Hypericum; Kaempferols; Quercetin; Rutin; Sitosterols

Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as β-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC50 values of 0.77, 0.74, and 16.6 μM, respectively.

Topics: Bauhinia; Cell Survival; Dose-Response Relationship, Drug; Flavonoids; Genes, Reporter; HCT116 Cells; HEK293 Cells; Humans; Inhibitory Concentration 50; Kaempferols; Luciferases; Methanol; Phytotherapy; Plant Extracts; Plant Leaves; Plants, Medicinal; Quercetin; Sitosterols; Solvents; Transfection; Wnt Signaling Pathway

Laetia suaveolens, known as "casinga-cheirosa", crude extract EB719 has previously shown cytotoxic activity against prostate cancer and squamous cell carcinoma. For the first time, seven molecules were isolated from its apolar-α-tocopherol (1) and sitosterol (2)-and polar-3-O-caffeoylquinic acid (3), 4-O-caffeoylquinic acid (4), 5-O-feruloylquinic acid (5), hyperoside (6), and isoquercitrin (7)-fractions. Acute toxicity was determined in a two-stage experiment: (1) a reduced number of Balb-c male mice received 5000 mg/kg of EB719 to allow evaluation of general activity and other 27 parameters, plus death, up to the establishment of non-lethal dose (NLD), as well as lethal dose 50% (LD50); (2) NLD was administered and diazepam introduced as reference drug. EB719 showed LD50=178.0 mg/kg, and NLD 156.3 mg/kg. In stage one EB719 did not influence general activity, but provoked impairment in grasp reflexes, tail squeeze and breathing; piloerection and cyanosis were increased. In stage two, alterations occurred in auricular reflex, piloerection and breathing after diazepam administration, but not in response to EB719. Intestinal hemorrhage caused by local bleeding was observed after necropsy, and may be the main cause of animals' death other than a systemic effect of the extract. Although the isolated compounds are biologically and pharmacologically active in both men and animal systems, it is premature to relate their occurrence in EB719 to the observed intestine hemorrhage in mice.

Topics: alpha-Tocopherol; Animals; Body Weight; Diazepam; Gastrointestinal Hemorrhage; Humans; Lethal Dose 50; Male; Mice; Mice, Inbred BALB C; Organ Size; Piloerection; Plant Extracts; Quercetin; Quinic Acid; Respiration; Salicaceae; Sitosterols

To study the chemical constituents from the leaf of Rhododendron przewalskii.. Isolate and purify compounds by chromatographic methods, and identify structures by spectroscopic methods.. Six compounds were isolated and identified as hyperin (1), avicularin (2), gossypetin-3-O-β-D-galactoside (3), quercetin (4), betuloside (5) and β-sitosterol (6).. Compounds 1-4 and 6 are isolated from Rhododendron przewalskii for the frist time.

Topics: Flavonoids; Plant Leaves; Quercetin; Rhododendron; Sitosterols