gamma-linolenic-acid and malic-acid

To optimize the production of linolenic acid by disrupted mycelia of Mortierella isabellina.. Effects of incubation conditions such as incubation time, pH of reaction mixture, concentration of Mg2+ or malate and incubation temperature on production of linolenic acid were studied. The production of gamma-linolenic acid reached 224 mg g-1 dry cells when the reaction mixture was composed of 1.0 g (dry mycelial mass) of disrupted mycelia of M. isabellina, 50 ml (50 mmol l(-1)) potassium phosphate buffer supplemented with 0.312 mmol l(-1) of Mg2+ and 10 mmol l(-1) of malate, pH 7.0 and incubated at 5 degrees C for 1 day.. Incubation temperature, concentration of Mg2+ and malate showed major effects on the increased linolenic acid production.. This study highlights conditions for increasing gamma-linolenic acid production by cell-free mycelia of M. isabellina and an insight into rapidly gaining high production of polyunsaturated fatty acids.

Topics: Culture Media; Fatty Acid Desaturases; gamma-Linolenic Acid; Magnesium; Malates; Mortierella; Mycelium; Temperature