gamma-cyclodextrin and 4-(aminosulfonyl)-7-fluoro-2-1-3-benzoxadiazole

gamma-cyclodextrin has been researched along with 4-(aminosulfonyl)-7-fluoro-2-1-3-benzoxadiazole* in 1 studies

Other Studies

1 other study(ies) available for gamma-cyclodextrin and 4-(aminosulfonyl)-7-fluoro-2-1-3-benzoxadiazole

Article	Year
Chiral separation of homocysteine by derivatization with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole followed by capillary electrophoresis using gamma-cyclodextrin. Journal of chromatography. A, 2000, Apr-28, Volume: 877, Issue:1-2 Homocysteine was derivatized with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F) to form an inclusion complex with cyclodextrin and to facilitate UV detection. ABD-homocysteine showed interaction with beta- and gamma-cyclodextrin in capillary electrophoresis at pH 2.25 as indicated by the decreased migration time. However, chiral separation of D,L-ABD-homocysteine was observed using gamma-CD only. Optimal separation was obtained at pH 2.25, 50 mM gamma-CD concentration, and 20 kV applied voltage. L-ABD-Homocysteine migrated faster than the D-isomer as demonstrated by a spiking experiment using dithiothreitol-reduced L-homocystine. Topics: Chromatography, High Pressure Liquid; Cyclodextrins; Electrophoresis, Capillary; Fluorescent Dyes; gamma-Cyclodextrins; Homocysteine; Hydrogen-Ion Concentration; Oxadiazoles; Spectrophotometry, Ultraviolet; Stereoisomerism	2000

Article

Year

Chiral separation of homocysteine by derivatization with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole followed by capillary electrophoresis using gamma-cyclodextrin.

Journal of chromatography. A, 2000, Apr-28, Volume: 877, Issue:1-2

Homocysteine was derivatized with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F) to form an inclusion complex with cyclodextrin and to facilitate UV detection. ABD-homocysteine showed interaction with beta- and gamma-cyclodextrin in capillary electrophoresis at pH 2.25 as indicated by the decreased migration time. However, chiral separation of D,L-ABD-homocysteine was observed using gamma-CD only. Optimal separation was obtained at pH 2.25, 50 mM gamma-CD concentration, and 20 kV applied voltage. L-ABD-Homocysteine migrated faster than the D-isomer as demonstrated by a spiking experiment using dithiothreitol-reduced L-homocystine.

Topics: Chromatography, High Pressure Liquid; Cyclodextrins; Electrophoresis, Capillary; Fluorescent Dyes; gamma-Cyclodextrins; Homocysteine; Hydrogen-Ion Concentration; Oxadiazoles; Spectrophotometry, Ultraviolet; Stereoisomerism

2000