gala-peptide has been researched along with stearic-acid* in 1 studies
1 other study(ies) available for gala-peptide and stearic-acid
| Article | Year |
|---|---|
Design and evaluation of a stearylated multicomponent peptide-siRNA nanocomplex for efficient cellular siRNA delivery.
To develop a new synthetic peptide-based nanoparticulate siRNA delivery system.. DEN-K(GALA)-TAT-K(STR) was generated by incorporating stearic acid into a multicomponent peptide (DEN-K(GALA)-TAT), containing a cationic poly-L-lysine dendron, an endosome-disrupting peptide GALA and a cell-penetrating peptide TAT(48-60). Its physicochemical characteristics, size, toxicity, cellular uptake and gene knockdown activity of the peptide/siRNA complexes were studied.. DEN-K(GALA)-TAT-K(STR) exhibited a pH-responsive behavior, which assists with endosomal escape. When siRNA was delivered by DEN-K(GALA)-TAT-K(STR), it showed a significantly enhanced cellular uptake, compared with the nonlipidic peptide. This system also displayed enhanced knockdown efficiency and reduced cytotoxicity over the widely used delivery system branched 25-kDa polyethyleneimine.. Our stearylated multicomponent delivery system has great potential as an efficient siRNA delivery vector. Topics: Cell-Penetrating Peptides; Green Fluorescent Proteins; HeLa Cells; Humans; Nanoparticles; Peptides; Polyethyleneimine; RNA Interference; RNA, Small Interfering; Stearic Acids; Transfection | 2017 |