g(m2)-ganglioside and 15-keto-5-8-11-13-eicosatetraenoic-acid

g(m2)-ganglioside has been researched along with 15-keto-5-8-11-13-eicosatetraenoic-acid* in 1 studies

Other Studies

1 other study(ies) available for g(m2)-ganglioside and 15-keto-5-8-11-13-eicosatetraenoic-acid

Article	Year
Monoclonal antibody directed to a Hanganutziu-Deicher active ganglioside, GM2 (NeuGc). Biochimica et biophysica acta, 1988, Feb-19, Volume: 958, Issue:3 Spleen cells from NZB mouse immunized with a membrane fraction of rabbit thymus tissue were fused with BALB/c 6-thioguanine-resistant myeloma cells, P3-X63-Ag8.653. One hybridoma clone (Y-2-HD-1) produced IgM immunoglobulin that bound to an N-glycolylneuraminic acid-containing GM2 ganglioside, GM2(NeuGc), which is known to be a Hanganutziu-Deicher antigen. The specificity of the Y-2-HD-1 monoclonal antibody was examined, using authentic glycosphingolipids structurally related to GM2(NeuGc), by means of an enzyme-linked immunosorbent assay and thin-layer chromatography/enzyme immunostaining, respectively. The monoclonal antibody was found to be highly specific to GM2(NeuGc) and the epitope was a non-reducing terminal GalNAc beta 1-4[NeuGc alpha 2-3]Gal structure. This monoclonal antibody (Y-2-HD-1) bound to native mouse erythrocytes, in which GM2(NeuGc) is a major ganglioside. These results indicate that GM2(NeuGc) is located on the surface of mouse erythrocytes. Topics: Animals; Antibodies, Monoclonal; Antibody Specificity; Antigens, Heterophile; Arachidonic Acids; Chromatography, Thin Layer; Enzyme-Linked Immunosorbent Assay; Epitopes; Erythrocytes; Fatty Acids, Unsaturated; Female; G(M2) Ganglioside; Gangliosides; Hybridomas; Immunoenzyme Techniques; Immunoglobulin M; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred NZB; Rabbits	1988

Article

Year

Monoclonal antibody directed to a Hanganutziu-Deicher active ganglioside, GM2 (NeuGc).

Biochimica et biophysica acta, 1988, Feb-19, Volume: 958, Issue:3

Spleen cells from NZB mouse immunized with a membrane fraction of rabbit thymus tissue were fused with BALB/c 6-thioguanine-resistant myeloma cells, P3-X63-Ag8.653. One hybridoma clone (Y-2-HD-1) produced IgM immunoglobulin that bound to an N-glycolylneuraminic acid-containing GM2 ganglioside, GM2(NeuGc), which is known to be a Hanganutziu-Deicher antigen. The specificity of the Y-2-HD-1 monoclonal antibody was examined, using authentic glycosphingolipids structurally related to GM2(NeuGc), by means of an enzyme-linked immunosorbent assay and thin-layer chromatography/enzyme immunostaining, respectively. The monoclonal antibody was found to be highly specific to GM2(NeuGc) and the epitope was a non-reducing terminal GalNAc beta 1-4[NeuGc alpha 2-3]Gal structure. This monoclonal antibody (Y-2-HD-1) bound to native mouse erythrocytes, in which GM2(NeuGc) is a major ganglioside. These results indicate that GM2(NeuGc) is located on the surface of mouse erythrocytes.

Topics: Animals; Antibodies, Monoclonal; Antibody Specificity; Antigens, Heterophile; Arachidonic Acids; Chromatography, Thin Layer; Enzyme-Linked Immunosorbent Assay; Epitopes; Erythrocytes; Fatty Acids, Unsaturated; Female; G(M2) Ganglioside; Gangliosides; Hybridomas; Immunoenzyme Techniques; Immunoglobulin M; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred NZB; Rabbits

1988