g(m1)-ganglioside and thiobarbituric-acid

g(m1)-ganglioside has been researched along with thiobarbituric-acid* in 1 studies

Other Studies

1 other study(ies) available for g(m1)-ganglioside and thiobarbituric-acid

Article	Year
Quantitative determination of sialic acid in the monosialoganglioside, GM1, by the thiobarbituric acid method. Analytical biochemistry, 1997, Mar-01, Volume: 246, Issue:1 Several methods for the quantitative analysis of sialic acids in glycoconjugates require that the sialic acid glycosidic bond be cleaved prior to assay. The conventional (L. Svennerholm, (1958) Acta Chem. Scand. 12, 547-554) procedure for the hydrolysis of complex carbohydrates such as gangliosides employs 0.1 n H+ at 80 degrees C for 60 min. Under these conditions, we find that the monosialoganglioside (GM1) yields less than 50% of the total sialic acid. However, 90% recovery of sialic acid was achieved by supplementing the hydrolysis mixture with 0.2% sodium dodecylsulfate (SDS) and increasing the temperature to 85 degrees C. During hydrolysis under these conditions, N-acetylneuraminic acid (NAN) is degraded at about 7% per hour. If the analytical values for GM1 are corrected for degradation, the recovery of NAN is essentially quantitative. Topics: Chromatography, Thin Layer; G(M1) Ganglioside; N-Acetylneuraminic Acid; Thiobarbiturates	1997

Article

Year

Quantitative determination of sialic acid in the monosialoganglioside, GM1, by the thiobarbituric acid method.

Analytical biochemistry, 1997, Mar-01, Volume: 246, Issue:1

Several methods for the quantitative analysis of sialic acids in glycoconjugates require that the sialic acid glycosidic bond be cleaved prior to assay. The conventional (L. Svennerholm, (1958) Acta Chem. Scand. 12, 547-554) procedure for the hydrolysis of complex carbohydrates such as gangliosides employs 0.1 n H+ at 80 degrees C for 60 min. Under these conditions, we find that the monosialoganglioside (GM1) yields less than 50% of the total sialic acid. However, 90% recovery of sialic acid was achieved by supplementing the hydrolysis mixture with 0.2% sodium dodecylsulfate (SDS) and increasing the temperature to 85 degrees C. During hydrolysis under these conditions, N-acetylneuraminic acid (NAN) is degraded at about 7% per hour. If the analytical values for GM1 are corrected for degradation, the recovery of NAN is essentially quantitative.

Topics: Chromatography, Thin Layer; G(M1) Ganglioside; N-Acetylneuraminic Acid; Thiobarbiturates

1997