g(m1)-ganglioside and formamide

g(m1)-ganglioside has been researched along with formamide* in 1 studies

Other Studies

1 other study(ies) available for g(m1)-ganglioside and formamide

Article	Year
Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors. Electrophoresis, 2018, Volume: 39, Issue:2 Developing tools for the study of protein carbohydrate interactions is an important goal in glycobiology. Cholera toxin inhibition is an interesting target in this context, as its inhibition may help to fight against cholera. For the study of novel ligands an affinity capillary electrophoresis (ACE) method was optimized and applied. The method uses unlabeled cholera toxin B-subunit (CTB) and unlabeled carbohydrate ligands based on ganglioside GM1-oligosaccharides (GM1os). In an optimized method at pH 4, adsorption of the protein to the capillary walls was prevented by a polybrene-dextran sulfate-polybrene coating. Different concentrations of the ligands were added to the BGE. CTB binding was observed by a mobility shift that could be used for dissociation constant (K Topics: Cholera Toxin; Electrophoresis, Capillary; Enzyme Inhibitors; Formamides; G(M1) Ganglioside; Oligosaccharides; Protein Binding	2018

Article

Year

Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors.

Electrophoresis, 2018, Volume: 39, Issue:2

Developing tools for the study of protein carbohydrate interactions is an important goal in glycobiology. Cholera toxin inhibition is an interesting target in this context, as its inhibition may help to fight against cholera. For the study of novel ligands an affinity capillary electrophoresis (ACE) method was optimized and applied. The method uses unlabeled cholera toxin B-subunit (CTB) and unlabeled carbohydrate ligands based on ganglioside GM1-oligosaccharides (GM1os). In an optimized method at pH 4, adsorption of the protein to the capillary walls was prevented by a polybrene-dextran sulfate-polybrene coating. Different concentrations of the ligands were added to the BGE. CTB binding was observed by a mobility shift that could be used for dissociation constant (K

Topics: Cholera Toxin; Electrophoresis, Capillary; Enzyme Inhibitors; Formamides; G(M1) Ganglioside; Oligosaccharides; Protein Binding

2018