fumarates and titanium-dioxide

A two-step synthesis is described for activating the surface of a fully hydrated hydrogel that is of interest as a possible scaffold for neural regeneration devices. The first step exploits the water content of the hydrogel and the hydrophobicity of the reaction solvent to create a thin oxide layer on the hydrogel surface using a common titanium or zirconium alkoxide. This layer serves as a reactive interface that enables rapid transformation of the hydrophilic, cell-nonadhesive hydrogel into either a highly hydrophobic surface by reaction with an alkylphosphonic acid, or into a cell-adhesive one using a (α,ω-diphosphono)alkane. Physically imprinting a mask ("debossing") into the hydrogel, followed by a two-step surface modification with a phosphonate, allows for patterning its surface to create spatially defined, cell-adhesive regions.

Topics: Animals; Cell Adhesion; Fumarates; Hydrogels; Hydrophobic and Hydrophilic Interactions; Mice; Microscopy; NIH 3T3 Cells; Polyethylene Glycols; Surface Properties; Titanium; Zirconium

This study tested the accuracy of tissue engineering scaffold rendering via the continuous digital light processing (cDLP) light-based additive manufacturing technology. High accuracy (i.e., <50 µm) allows the designed performance of features relevant to three scale spaces: cell-scaffold, scaffold-tissue, and tissue-organ interactions. The biodegradable polymer poly (propylene fumarate) was used to render highly accurate scaffolds through the use of a dye-initiator package, TiO2 and bis (2,4,6-trimethylbenzoyl)phenylphosphine oxide. This dye-initiator package facilitates high accuracy in the Z dimension. Linear, round, and right-angle features were measured to gauge accuracy. Most features showed accuracies between 5.4-15% of the design. However, one feature, an 800 µm diameter circular pore, exhibited a 35.7% average reduction of patency. Light scattered in the x, y directions by the dye may have reduced this feature's accuracy. Our new fine-grained understanding of accuracy could be used to make further improvements by including corrections in the scaffold design software. Successful cell attachment occurred with both canine and human mesenchymal stem cells (MSCs). Highly accurate cDLP scaffold rendering is critical to the design of scaffolds that both guide bone regeneration and that fully resorb. Scaffold resorption must occur for regenerated bone to be remodeled and, thereby, achieve optimal strength.

Topics: Animals; Bone Marrow Cells; Bone Regeneration; Cell Adhesion; Coloring Agents; Dogs; Fumarates; Humans; Light; Mesenchymal Stem Cells; Polypropylenes; Tissue Engineering; Tissue Scaffolds; Titanium

Rigid, biodegradable photopolymer scaffolds were coated with titanate nanotubes (TNTs) by using a spin-coating method. TNTs were synthesized by a hydrothermal process at 150 °C under 4.7 bar ambient pressure. The biodegradable photopolymer scaffolds were produced by mask-assisted excimer laser photocuring at 308 nm. For scaffold coating, a stable ethanolic TNT sol was prepared by a simple colloid chemical route without the use of any binding compounds or additives. Scanning electron microscopy along with elemental analysis revealed that the scaffolds were homogenously coated by TNTs. The developed TNT coating can further improve the surface geometry of fabricated scaffolds, and therefore it can further increase the cell adhesion.

Topics: Biodegradation, Environmental; Coated Materials, Biocompatible; Fumarates; Light; Nanotubes; Polymerization; Polymers; Polypropylenes; Porosity; Powders; Tissue Scaffolds; Titanium; X-Ray Diffraction