fructooligosaccharide has been researched along with levanbiose* in 3 studies
3 other study(ies) available for fructooligosaccharide and levanbiose
| Article | Year |
|---|---|
Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
Prebiotics are known for their ability to modulate the composition of the human microbiome and mediate health-promoting benefits. Endo-levanases, which hydrolyze levan into short-chain FOS, could be used for the production of levan-based prebiotics. The novel endo-levanase (LevB Topics: Azotobacter; Bacterial Proteins; Disaccharides; Escherichia coli; Fructans; Fructose; Gene Expression; Gluconobacter; Glycoside Hydrolases; Hexosyltransferases; Humans; Hydrolysis; Oligosaccharides; Phleum; Prebiotics; Recombinant Proteins; Sucrose | 2021 |
The Structure of Sucrose-Soaked Levansucrase Crystals from
Given its potential role in the synthesis of novel prebiotics and applications in the pharmaceutical industry, a strong interest has developed in the enzyme levansucrase (LSC, EC 2.4.1.10). LSC catalyzes both the hydrolysis of sucrose (or sucroselike substrates) and the transfructosylation of a wide range of acceptors. LSC from the Gram-negative bacterium Topics: Bacterial Proteins; Binding Sites; Crystallization; Crystallography, X-Ray; Disaccharides; Erwinia; Fructans; Hexosyltransferases; Models, Molecular; Oligosaccharides; Protein Conformation; Sucrose | 2019 |
Understanding the transfer reaction network behind the non-processive synthesis of low molecular weight levan catalyzed by Bacillus subtilis levansucrase.
Under specific reaction conditions, levansucrase from Bacillus subtilis (SacB) catalyzes the synthesis of a low molecular weight levan through the non-processive elongation of a great number of intermediates. To deepen understanding of the polymer elongation mechanism, we conducted a meticulous examination of the fructooligosaccharide profile evolution during the levan synthesis. As a result, the formation of primary and secondary intermediates series in different reaction stages was observed. The origin of the series was identified through comparison with product profiles obtained in acceptor reactions employing levanbiose, blastose, 1-kestose, 6-kestose, and neo-kestose, and supported with the isolation and NMR analyses of some relevant products, demonstrating that all of them are inherent products during levan formation from sucrose. These results allowed to establish the network of fructosyl transfer reactions involved in the non-processive levan synthesis. Overall, our results reveal how the relaxed acceptor specificity of SacB during the initial steps of the synthesis is responsible for the formation of several levan series, which constitute the final low molecular weight levan distribution. Topics: Bacillus subtilis; Catalysis; Disaccharidases; Disaccharides; Fructans; Hexosyltransferases; Kinetics; Molecular Weight; Oligosaccharides; Sucrose; Trisaccharides | 2018 |