fructooligosaccharide has been researched along with galactosucrose* in 2 studies
2 other study(ies) available for fructooligosaccharide and galactosucrose
| Article | Year |
|---|---|
Investigation of biocatalytic production of lactosucrose and fructooligosaccharides using levansucrases and dairy by-products as starting materials.
Selected levansucrases (LSs) were investigated for their ability to catalyze the transfructosylation of lactose/sucrose into lactosucrose and fructooligosaccharides (FOSs). Additionally, dairy by-products, including whey permeate (WP) and milk permeate (MP), were assessed for their effectiveness as lactose sources. LSs from Gluconobacter oxydans (LS1), Vibrio natriegens (LS2), Novosphingobium aromaticivorans (LS3), and Burkholderia graminis (LS4) were utilized in three transfructosylation reactions that combined sucrose with either lactose, WP, or MP. All LSs demonstrated a higher transfructosylation activity than hydrolytic one, except for V. natriegens LS2 in the presence of sucrose and MP/sucrose. Furthermore, the bioconversion efficiency of lactose/sucrose into lactosucrose and FOSs exhibited varying time courses and end-product profiles. Both the acceptor specificity of LS and the thermodynamic equilibrium of its reaction modulated the end-product profile. V. natriegens LS2 resulted in the highest lactosucrose production of 328 and 251 g/L with lactose/sucrose and WP/sucrose, respectively. Our results revealed the potential of LS-catalyzed transfructosylation for the biocatalytic production of both lactosucrose and FOSs from abundant biomasses. Topics: Lactose; Sucrose | 2023 |
Comparison of digestibility and breath hydrogen gas excretion of fructo-oligosaccharide, galactosyl-sucrose, and isomalto-oligosaccharide in healthy human subjects.
To clarify the difference of digestibility in the small intestine among fructo-oligosaccharide (FOS), galactosyl-sucrose (GS), and isomalto-oligosaccharide (IMO) using breath hydrogen test.. The first step: screening test of breath hydrogen excretion and FOS tolerance test to select the subjects. The second step: breath hydrogen test of three kinds of oligosaccharides, carried out using precautionary regulations. The ingestion order was 10 g of FOS, GS, and IMO, with increases, at 1-week interval, up to 20 g, respectively. Breath gas was collected before, at 20 min intervals from 40 to 120 min after, and at 30 min intervals from 120 min to 7 h after ingestion of test substance.. Laboratory of Public Health Nutrition, Department of Nutrition and Health Sciences, Siebold University of Nagasaki, Nagasaki, Japan.. A total of nine males (average: age 25.7+/-3.5 y, weight 61.9+/-8.8 kg, height 170.0+/-6.0 cm) and 29 females (average: 23.1+/-7.2 y, 52.9+/-5.3 kg, 157.5+/-5.1 cm) from the University of Tokyo and Siebold University of Nagasaki.. Breath hydrogen excretion from end-expiratory gas.. : Breath hydrogen of FOS was more remarkably excreted than that of GS; that of IMO was slight; and that of AUC (10 g) was significantly different. FOS was 9768+/-3253 ppm, GS was 3662+/-2632 ppm, and IMO was 831+/-1154 ppm. A dose dependence was observed at doses between 10 and 20 g of FOS and GS, and the initial time of 20 g was earlier than that of 10 g.. FOS was not hydrolyzed, GS was slightly hydrolyzed, and IMO was readily hydrolyzed by small intestinal enzymes. H(2) gas reflected fermentability in the large intestine.. Siebold University of Nagasaki. Topics: Adult; Analysis of Variance; Breath Tests; Digestion; Female; Humans; Hydrogen; Intestine, Small; Isomaltose; Male; Oligosaccharides; Reference Values; Time Factors; Trisaccharides | 2003 |