forsythiaside and acteoside

Topics: Antiviral Agents; Binding Sites; Coronavirus 3C Proteases; Coronavirus Protease Inhibitors; Drug Evaluation, Preclinical; Glucosides; Glycosides; Hydrogen Bonding; Molecular Docking Simulation; Molecular Dynamics Simulation; Phenols

To establish a new method for simultaneous determination of shanzhiside methyl ester, chlorogenic acid, 8-O-acetyl shanzhiside methylester, forsythiaside B, rutin, acteoside and galuteolin in Lamiophlomis rotata.. Separation was performed on a Welchrom-C18 chromatographic column with acetonitrile-0.1% orthophosphoric acid as mobile phasewith gradient elution. The flow rate was 1.0 mL x min(-1). The column temperature was 30 degrees C, and the detection wavelength was set at 238 nm, 330 nm and 350 nm.. The seven compounds were well separated with good linear correlations. The mean recoveries of seven compounds were 96.47%-102.2% (RSD 0.70%-2.2%).. There were good correlations among the seven compounds in the samples of aerial parts. The mean sum of shanzhiside methyl ester and 8-O-acetyl shanzhiside methylester in samples of aerial parts is 1.44%. The aerial parts have more kinds of composition and with higher content than that of underground parts in L. rotata, which was reasonable for the resonable use of the aerial part as medicinal part. The method was simple, repeatable and stable, which could be used for identification and quality evaluation of L. rotata.

Topics: Chlorogenic Acid; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Glucosides; Glycosides; Lamiaceae; Methyl Ethers; Phenols; Plant Components, Aerial; Plants, Medicinal; Pyrans; Rutin

Three phenylethanoid glycosides, forsythoside B (1), phlinoside C (2) and verbascoside (3), were isolated from the methanol extract of the leaves of Phlomis lanceolata, an Iranian medicinal plant, by reversed-phase preparative high-performance liquid chromatography (HPLC), and the structures of these compounds were elucidated conclusively by ultraviolet (UV), mass spectrometry (MS) and a series of 1D and 2D nuclear magnetic resonance (NMR) analyses. The antibacterial properties of 1-3 against five multi-drug-resistant (MDR) strains of Staphylococcus aureus have been assessed by the rapid and robust microtitre-plate-based serial dilution method. While compounds 1 and 3 showed considerable activities against all five strains, compound 2 was inactive at the test concentrations.

Topics: Anti-Bacterial Agents; Chromatography, High Pressure Liquid; Drug Resistance, Multiple, Bacterial; Glucosides; Glycosides; Magnetic Resonance Spectroscopy; Mass Spectrometry; Microbial Sensitivity Tests; Molecular Structure; Phenols; Phlomis; Plant Leaves; Spectrophotometry, Ultraviolet; Staphylococcus aureus