formazans and indoxyl

formazans has been researched along with indoxyl* in 1 studies

Other Studies

1 other study(ies) available for formazans and indoxyl

Article	Year
Visualization of alkaline phosphatase-labelled antibodies on immunoblots by means of formazan staining using indoxyl phosphate and thiazolyl blue. Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, 1990, Volume: 1, Issue:5 Alkaline phosphatase-conjugated secondary antibodies on blotting membranes were visualized by means of a modified formazan staining method (Hodson & Skillen 1988). It is based on the standard 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue tetrazolium method, but employs different buffer conditions and thiazolyl blue instead of Nitroblue. It gave a sensitivity of 1 ng albumin in a 12 mm2 dot and less background staining on dot blots than the standard method. The new protocol provides an advantage with regard to ease of staining and preparation of reagents while it equals the former method with respect to sensitivity. Topics: Alkaline Phosphatase; Antibodies; Collodion; Electrophoresis, Polyacrylamide Gel; Erythrocyte Membrane; Formazans; Immunoblotting; Indoles; Polyvinyl Chloride; Sensitivity and Specificity; Serum Albumin; Staining and Labeling; Tetrazolium Salts; Thiazoles	1990

Article

Year

Visualization of alkaline phosphatase-labelled antibodies on immunoblots by means of formazan staining using indoxyl phosphate and thiazolyl blue.

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, 1990, Volume: 1, Issue:5

Alkaline phosphatase-conjugated secondary antibodies on blotting membranes were visualized by means of a modified formazan staining method (Hodson & Skillen 1988). It is based on the standard 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue tetrazolium method, but employs different buffer conditions and thiazolyl blue instead of Nitroblue. It gave a sensitivity of 1 ng albumin in a 12 mm2 dot and less background staining on dot blots than the standard method. The new protocol provides an advantage with regard to ease of staining and preparation of reagents while it equals the former method with respect to sensitivity.

Topics: Alkaline Phosphatase; Antibodies; Collodion; Electrophoresis, Polyacrylamide Gel; Erythrocyte Membrane; Formazans; Immunoblotting; Indoles; Polyvinyl Chloride; Sensitivity and Specificity; Serum Albumin; Staining and Labeling; Tetrazolium Salts; Thiazoles

1990