fm1-43 and glycylphenylalanine-2-naphthylamide

The purpose of this study was to determine whether lysosome trafficking and targeting of acid sphingomyelinase (ASMase) to this organelle contribute to the formation of lipid raft (LR) signaling platforms in the membrane of coronary arterial endothelial cells (CAECs).. By measurement of fluorescent resonance energy transfer (FRET), it was found that in FasL-stimulated CAECs, membrane lamp1 (a lysosome marker protein) or Fas and GM1 (a LR marker) were trafficking together. Cofocal colocalization assay showed that ceramide was enriched in these LR platforms. Further studies demonstrated that these ceramide molecules in LR platforms were colocalized with ASMase, a ceramide producing enzyme. Fluorescence imaging of living CAECs loaded with lysosomal specific dyes demonstrated that lysosomes fused with membrane on FasL stimulation. In the presence of lysosome function inhibitors, bafilomycin (Baf) or glycyl-L-phenylalanine-beta-naphthylamide (GPN), these FasL-induced changes were abolished. Moreover, this FasL-induced formation of LR platforms was also blocked in ECs transfected with siRNA of sortilin, an intracellular transporter for targeting of ASMase to lysosomes. Functionally, FasL-induced impairment of vasodilator response was reversed by lysosomal inhibitors or sortilin gene silencing.. Lysosomal trafficking and targeting of ASMase are importantly involved in LRs clustering in ECs membrane, leading to the formation of signaling platforms or signalosomes.

Topics: Adaptor Proteins, Vesicular Transport; Animals; Bradykinin; Cattle; Caveolin 1; Cells, Cultured; Ceramides; Coronary Vessels; Dipeptides; Endothelial Cells; Fas Ligand Protein; fas Receptor; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; G(M1) Ganglioside; Lysosomal-Associated Membrane Protein 1; Lysosomes; Macrolides; Membrane Fusion; Membrane Glycoproteins; Membrane Microdomains; Microscopy, Confocal; Nerve Tissue Proteins; Protein Transport; Pyridinium Compounds; Quaternary Ammonium Compounds; RNA Interference; RNA, Small Interfering; Signal Transduction; Sphingomyelin Phosphodiesterase; Vasodilation