fm1-43 has been researched along with diacetylfluorescein* in 1 studies
1 other study(ies) available for fm1-43 and diacetylfluorescein
| Article | Year |
|---|---|
Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus.
The isolation of vacuoles is an essential step to unravel the important and complex functions of this organelle in plant physiology. Here, we describe a method for the isolation of vacuoles from Catharanthus roseus leaves involving a simple procedure for the isolation of protoplasts, and the application of a controlled osmotic/thermal shock to the naked cells, leading to the release of intact vacuoles, which are subsequently purified by density gradient centrifugation. The purity of the isolated intact vacuoles is assayed by microscopy, western blotting, and measurement of vacuolar (V)-H Topics: Benzenesulfonates; Blotting, Western; Catharanthus; Cell Fractionation; Enzyme Assays; Fluoresceins; Fluorescent Dyes; Hydrolysis; Microscopy, Fluorescence; Neutral Red; Optical Imaging; Osmotic Pressure; Plant Leaves; Plant Proteins; Plants, Medicinal; Protoplasts; Pyridinium Compounds; Quaternary Ammonium Compounds; Staining and Labeling; Vacuolar Proton-Translocating ATPases; Vacuoles | 2018 |