fluvoxamine has been researched along with n-hexane* in 1 studies
1 other study(ies) available for fluvoxamine and n-hexane
Article | Year |
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A sensitive HPLC method for the determination of fluoxetine and norfluoxetine in human plasma with fluorescence detection.
A selective, sensitive, and precise HPLC method for the simultaneous determination of fluoxetine (FL) and its N-demethylated metabolite norfluoxetine (NFL) in human plasma has been developed. Following extraction with n-hexane, FL, NFL, and fluvoxamine (internal standard) were derivatized with 7-chloro-4-nitrobenzofurazan (NBD-Cl) under weakly alkaline conditions. NBD derivatives were extracted with chloroform after acidification and chromatographed on a reversed-phase column with gradient elution using acetonitrile and 0.1 mol/L nitric acid (pH 3) solution. Calibration curves were linear over the range of 1.0-100.0 ng/mL and 0.1-50.0 ng/mL for FL and NFL, respectively, with inter- and intraassay precision given by a relative standard deviation (RSD%) of less than 9.2%. The lower limits of quantification were 1.0 ng/mL for FL and 0.1 ng/mL for NFL. Recoveries of FL and NFL from plasma at three different concentrations were assessed. Average recovery was about 100% for both substances. The assay was applied to pharmacokinetic study in 2 healthy volunteers after a single oral administration of 40 mg of FL. Topics: Administration, Oral; Area Under Curve; Benzofurans; Chromatography, High Pressure Liquid; Drug Administration Schedule; Drug Monitoring; Fluoxetine; Fluvoxamine; Half-Life; Hexanes; Humans; Kinetics; Reference Standards; Sensitivity and Specificity | 2005 |