flonicamid has been researched along with 3-cyanoalanine* in 1 studies
1 other study(ies) available for flonicamid and 3-cyanoalanine
| Article | Year |
|---|---|
Characterization of nitrilases from Variovorax boronicumulans that functions in insecticide flonicamid degradation and β-cyano-L-alanine detoxification.
To characterize the functions of nitrilases of Variovorax boronicumulans CGMCC 4969 and evaluate flonicamid (FLO) degradation and β-cyano-L-alanine (Ala(CN)) detoxification by this bacterium.. Variovorax boronicumulans CGMCC 4969 nitrilases (NitA and NitB) were purified, and substrate specificity assay indicated that both of them degraded insecticide FLO to N-(4-trifluoromethylnicotinoyl)glycinamide (TFNG-AM) and 4-(trifluoromethyl)nicotinol glycine (TFNG). Ala(CN), a plant detoxification intermediate, was hydrolysed by NitB. Escherichia coli overexpressing NitA and NitB degraded 41.2 and 93.8% of FLO (0.87 mmol·L. CGMCC 4969 efficiently degraded FLO mediated by NitA and NitB; NitB was involved in Ala(CN) detoxification.. This study promotes our understanding of versatile functions of nitrilases from CGMCC 4969 that is promising for environmental remediation. Topics: Alanine; Aminohydrolases; Comamonadaceae; Escherichia coli; Insecticides; Niacinamide | 2022 |