flavin-adenine-dinucleotide and aklavinone

The gene encoding aklavinone 11-hydroxylase of Streptomyces peucetius subsp. caesius ATCC 27952 was cloned and sequenced. The deduced amino acid sequence of the gene contains at least two common motifs of well-conserved amino acid sequences of several flavin-type bacterial hydroxylases. The hydroxylase gene is apparently transcribed from a single transcriptional start point. The phenotype of a dnrF mutant generated by gene disruption supports the idea that the dnrF gene encodes aklavinone 11-hydroxylase.

Topics: Amino Acid Sequence; Aryl Hydrocarbon Hydroxylases; Base Sequence; Cloning, Molecular; Flavin-Adenine Dinucleotide; Genes, Fungal; Molecular Sequence Data; NAD; Naphthacenes; Sequence Analysis, DNA; Streptomyces; Transcription, Genetic