flavin-adenine-dinucleotide and 5-11-methenyltetrahydrohomofolate

flavin-adenine-dinucleotide has been researched along with 5-11-methenyltetrahydrohomofolate* in 11 studies

Reviews

1 review(s) available for flavin-adenine-dinucleotide and 5-11-methenyltetrahydrohomofolate

ArticleYear
The structure of photolyase: using photon energy for DNA repair.
    Science (New York, N.Y.), 1995, Jun-30, Volume: 268, Issue:5219

    Topics: Cloning, Molecular; Crystallography, X-Ray; Deoxyribodipyrimidine Photo-Lyase; DNA Damage; DNA Repair; Escherichia coli; Flavin-Adenine Dinucleotide; Folic Acid; Light; Photosynthesis; Pyrimidine Dimers; Ultraviolet Rays

1995

Other Studies

10 other study(ies) available for flavin-adenine-dinucleotide and 5-11-methenyltetrahydrohomofolate

ArticleYear
Direct determination of resonance energy transfer in photolyase: structural alignment for the functional state.
    The journal of physical chemistry. A, 2014, Nov-13, Volume: 118, Issue:45

    Photoantenna is essential to energy transduction in photoinduced biological machinery. A photoenzyme, photolyase, has a light-harvesting pigment of methenyltetrahydrofolate (MTHF) that transfers its excitation energy to the catalytic flavin cofactor FADH¯ to enhance DNA-repair efficiency. Here we report our systematic characterization and direct determination of the ultrafast dynamics of resonance energy transfer from excited MTHF to three flavin redox states in E. coli photolyase by capturing the intermediates formed through the energy transfer and thus excluding the electron-transfer quenching pathway. We observed 170 ps for excitation energy transferring to the fully reduced hydroquinone FADH¯, 20 ps to the fully oxidized FAD, and 18 ps to the neutral semiquinone FADH(•), and the corresponding orientation factors (κ(2)) were determined to be 2.84, 1.53 and 1.26, respectively, perfectly matching with our calculated theoretical values. Thus, under physiological conditions and over the course of evolution, photolyase has adopted the optimized orientation of its photopigment to efficiently convert solar energy for repair of damaged DNA.

    Topics: Deoxyribodipyrimidine Photo-Lyase; Energy Transfer; Escherichia coli; Escherichia coli Proteins; Flavin-Adenine Dinucleotide; Folic Acid; Oxidation-Reduction; Riboflavin; Spectrum Analysis

2014
Genetic and molecular characterization of a cryptochrome from the filamentous fungus Neurospora crassa.
    Eukaryotic cell, 2010, Volume: 9, Issue:5

    In plants and animals, cryptochromes function as either photoreceptors or circadian clock components. We have examined the cryptochrome from the filamentous fungus Neurospora crassa and demonstrate that Neurospora cry encodes a DASH-type cryptochrome that appears capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). The cry transcript and CRY protein levels are strongly induced by blue light in a wc-1-dependent manner, and cry transcript is circadianly regulated, with a peak abundance opposite in phase to frq. Neither deletion nor overexpression of cry appears to perturb the free-running circadian clock. However, cry disruption knockout mutants show a small phase delay under circadian entrainment. Using electrophoretic mobility shift assays (EMSA), we show that CRY is capable of binding single- and double-stranded DNA (ssDNA and dsDNA, respectively) and ssRNA and dsRNA. Whole-genome microarray experiments failed to identify substantive transcriptional regulatory activity of cry under our laboratory conditions.

    Topics: Amino Acid Sequence; Amino Acids; Binding Sites; Biological Clocks; Circadian Rhythm; Conserved Sequence; Cryptochromes; DNA, Fungal; Escherichia coli; Flavin-Adenine Dinucleotide; Folic Acid; Fungal Proteins; Gene Expression Regulation, Fungal; Light; Molecular Sequence Data; Mutation; Neurospora crassa; Phenotype; Protein Binding; Pyrimidine Dimers; RNA, Fungal; RNA, Messenger; Time Factors

2010
Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna.
    Journal of molecular biology, 2007, Feb-23, Volume: 366, Issue:3

    Cryptochromes are almost ubiquitous blue-light receptors and act in several species as central components of the circadian clock. Despite being evolutionary and structurally related with DNA photolyases, a class of light-driven DNA-repair enzymes, and having similar cofactor compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its crystal structure determined at 1.9 Angstroms resolution shows cryptochrome 3 in a dimeric state with the antenna cofactor 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 Angstroms to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a residue crucial for MTHF-binding, E149, was replaced by site-directed mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional antenna for the photoreduction of FAD.

    Topics: Alanine; Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Binding Sites; Cryptochromes; Deoxyribodipyrimidine Photo-Lyase; Electrons; Flavin-Adenine Dinucleotide; Folic Acid; Glutamine; Light-Harvesting Protein Complexes; Models, Molecular; Molecular Sequence Data; Mutant Proteins; Protein Structure, Secondary; Spectrophotometry; Static Electricity; Structure-Activity Relationship

2007
Resonance Raman spectroscopic investigation of the light-harvesting chromophore in escherichia coli photolyase and Vibrio cholerae cryptochrome-1.
    Biochemistry, 2007, Mar-27, Volume: 46, Issue:12

    Photolyases and cryptochromes are flavoproteins that belong to the class of blue-light photoreceptors. They usually bind two chromophores: flavin adenine dinucleotide (FAD), which forms the active site, and a light-harvesting pigment, which is a 5,10-methenyltetrahydrofolate polyglutamate (MTHF) in most cases. In Escherichia coli photolyase (EcPhr), the MTHF cofactor is present in substoichiometric amounts after purification, while in Vibrio cholerae cryptochrome-1 (VcCry1) the MTHF cofactor is bound more strongly and is present at stoichiometric levels after purification. In this paper, we have used resonance Raman spectroscopy to monitor the effect of loss of MTHF on the protein-FAD interactions in EcPhr and to probe the protein-MTHF interactions in both EcPhr and VcCry1. We find that removal of MTHF does not perturb protein-FAD interactions, suggesting that it may not affect the physicochemical properties of FAD in EcPhr. Our data demonstrate that the pteridine ring of MTHF in EcPhr has different interactions with the protein matrix than that of MTHF in VcCry1. Comparison to solution resonance Raman spectra of MTHF suggests that the carbonyl of its pteridine ring in EcPhr experiences stronger hydrogen bonding and a more polar environment than in VcCry1, but that hydrogen bonding to the pteridine ring amine hydrogens is stronger in VcCry-1. These differences in hydrogen bonding may account for the higher binding affinity of MTHF in VcCry1 compared to EcPhr.

    Topics: Bacterial Proteins; Cryptochromes; Escherichia coli; Flavin-Adenine Dinucleotide; Flavoproteins; Folic Acid; Hydrogen Bonding; Photoreceptors, Microbial; Protein Binding; Protein Structure, Secondary; Spectrum Analysis, Raman; Vibrio cholerae

2007
Absorption and fluorescence spectroscopic characterization of cryptochrome 3 from Arabidopsis thaliana.
    Journal of photochemistry and photobiology. B, Biology, 2006, Oct-02, Volume: 85, Issue:1

    The blue light photoreceptor cryptochrome 3 (cry3) from Arabidopsis thaliana was characterized at room temperature in vitro in aqueous solution by optical absorption and emission spectroscopic studies. The protein non-covalently binds the chromophores flavin adenine dinucleotide (FAD) and N5,N10-methenyl-5,6,7,8-tetrahydrofolate (MTHF). In the dark-adapted state of cry3, the bound FAD is present in the oxidized form (FAD(ox), ca. 38.5%), in the semiquinone form (FADH., ca. 5%), and in the fully reduced neutral form (FAD(red)H2) or fully reduced anionic form (FAD(red)H-, ca. 55%). Some amount of FAD (ca. 1.5%) in the oxidized state remains unbound probably caused by chromophore release and/or denaturation. Förster-type energy transfer from MTHF to FAD(ox) is observed. Photo-excitation reversibly modifies the protein conformation causing a slight rise of the MTHF absorption strength and an increase of the MTHF fluorescence efficiency (efficient protein conformation photo-cycle). Additionally there occurs reversible reduction of bound FAD(ox) to FAD(red)H2 (or FAD(red)H-, FAD(ox) photo-cycle of moderate efficiency), reversible reduction of FADH. to FAD(red)H2 (or FAD(red)H-, FADH. photo-cycle of high efficiency), and modification of re-oxidable FAD(red)H2 (or FAD(red)H-) to permanent FAD(red)H2 (or FAD(red)H-) with low quantum efficiency. Photo-excitation of MTHF causes the reversible formation of a MTHF species (MTHF', MTHF photo-cycle, moderate quantum efficiency) with slow recovery to the initial dark state, and also the formation of an irreversible photoproduct (MTHF'').

    Topics: Absorption; Arabidopsis; Arabidopsis Proteins; Cryptochromes; Deoxyribodipyrimidine Photo-Lyase; Electron Transport; Energy Transfer; Flavin-Adenine Dinucleotide; Flavoproteins; Folic Acid; Quinones; Spectrometry, Fluorescence; Temperature; Time Factors

2006
Crystal structure of cryptochrome 3 from Arabidopsis thaliana and its implications for photolyase activity.
    Proceedings of the National Academy of Sciences of the United States of America, 2006, Nov-21, Volume: 103, Issue:47

    Cryptochromes use near-UV/blue light to regulate a variety of growth and adaptive process. Recent biochemical studies demonstrate that the Cryptochrome-Drosophila, Arabidopsis, Synechocystis, Human (Cry-DASH) subfamily of cryptochromes have photolyase activity exclusively for single-stranded cyclobutane pyrimidine dimer (CPD)-containing DNA substrate [Selby C, Sancar A (2006) Proc Natl Acad Sci USA 103:17696-17700]. The crystal structure of cryptochrome 3 from Arabidopsis thaliana (At-Cry3), a member of the Cry-DASH proteins, at 2.1 A resolution, reveals that both the light-harvesting cofactor 5,10-methenyl-tetrahydrofolyl-polyglutamate (MTHF) and the catalytic cofactor flavin adenine dinucleotide (FAD) are noncovalently bound to the protein. The residues responsible for binding of MTHF in At-Cry3 are not conserved in Escherichia coli photolyase but are strongly conserved in the Cry-DASH subfamily of cryptochromes. The distance and orientation between MTHF and flavin adenine dinucleotide in At-Cry3 is similar to that of E. coli photolyase, in conjunction with the presence of electron transfer chain, suggesting the conservation of redox activity in At-Cry3. Two amino acid substitutions and the penetration of three charged side chains into the CPD-binding cavity in At-Cry3 alter the hydrophobic environment that is accommodating the hydrophobic sugar ring and thymine base moieties in class I CPD photolyases. These changes most likely make CPD binding less energetically favorable and, hence, insufficient to compete with pairing and stacking interactions between the CPD and the duplex DNA substrate. Thus, Cry-DASH subfamily proteins may be unable to stabilize CPD flipped out from the duplex DNA substrate but may be able to preserve the DNA repair activity toward single-stranded CPD-containing DNA substrate.

    Topics: Amino Acid Sequence; Animals; Arabidopsis; Arabidopsis Proteins; Binding Sites; Coenzymes; Cryptochromes; Crystallography, X-Ray; Deoxyribodipyrimidine Photo-Lyase; Electron Transport; Flavin-Adenine Dinucleotide; Folic Acid; Humans; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Sequence Alignment

2006
Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy.
    The FEBS journal, 2005, Volume: 272, Issue:8

    Cyclobutane-type pyrimidine dimers generated by ultraviolet irradiation of DNA can be cleaved by DNA photolyase. The enzyme-catalysed reaction is believed to be initiated by the light-induced transfer of an electron from the anionic FADH- chromophore of the enzyme to the pyrimidine dimer. In this contribution, first infrared experiments using a novel E109A mutant of Escherichia coli DNA photolyase, which is catalytically active but unable to bind the second cofactor methenyltetrahydrofolate, are described. A stable blue-coloured form of the enzyme carrying a neutral FADH radical cofactor can be interpreted as an intermediate analogue of the light-driven DNA repair reaction and can be reduced to the enzymatically active FADH- form by red-light irradiation. Difference Fourier transform infrared (FT-IR) spectroscopy was used to monitor vibronic bands of the blue radical form and of the fully reduced FADH- form of the enzyme. Preliminary band assignments are based on experiments with 15N-labelled enzyme and on experiments with D2O as solvent. Difference FT-IR measurements were also used to observe the formation of thymidine dimers by ultraviolet irradiation and their repair by light-driven photolyase catalysis. This study provides the basis for future time-resolved FT-IR studies which are aimed at an elucidation of a detailed molecular picture of the light-driven DNA repair process.

    Topics: Bacillus subtilis; Catalysis; Deoxyribodipyrimidine Photo-Lyase; DNA Damage; DNA Repair; Enzyme Activation; Escherichia coli; Flavin-Adenine Dinucleotide; Folic Acid; Light; Mutation; Photochemistry; Spectroscopy, Fourier Transform Infrared; Thymine; Uridine

2005
Ultrafast dynamics of resonance energy transfer in cryptochrome.
    Journal of the American Chemical Society, 2005, Jun-08, Volume: 127, Issue:22

    In this communication, we report the ultrafast dynamics of resonance energy transfer in a blue-light photoreceptor, Vibrio cholerae cryptochrome. The transfer was observed to occur in 60 ps. We also studied the local rigidity and solvation around the binding site of the photoantenna molecule. The results for the first time show energy transfer in cryptochrome suggesting some mechanistic similarities between photolyase that repairs damaged DNA and cryptochrome that mediates blue-light signaling.

    Topics: Anisotropy; Cryptochromes; Deoxyribodipyrimidine Photo-Lyase; Energy Transfer; Flavin-Adenine Dinucleotide; Flavins; Flavoproteins; Folic Acid; Kinetics; Oxidation-Reduction; Photochemistry; Photoreceptor Cells; Spectrometry, Fluorescence; Vibrio cholerae

2005
Crystal structure of DNA photolyase from Escherichia coli.
    Science (New York, N.Y.), 1995, Jun-30, Volume: 268, Issue:5219

    Photolyase repairs ultraviolet (UV) damage to DNA by splitting the cyclobutane ring of the major UV photoproduct, the cis, syn-cyclobutane pyrimidine dimer (Pyr <> Pyr). The reaction is initiated by blue light and proceeds through long-range energy transfer, single electron transfer, and enzyme catalysis by a radical mechanism. The three-dimensional crystallographic structure of DNA photolyase from Escherichia coli is presented and the atomic model was refined to an R value of 0.172 at 2.3 A resolution. The polypeptide chain of 471 amino acids is folded into an amino-terminal alpha/beta domain resembling dinucleotide binding domains and a carboxyl-terminal helical domain; a loop of 72 residues connects the domains. The light-harvesting cofactor 5,10-methenyltetrahydrofolylpolyglutamate (MTHF) binds in a cleft between the two domains. Energy transfer from MTHF to the catalytic cofactor flavin adenine dinucleotide (FAD) occurs over a distance of 16.8 A. The FAD adopts a U-shaped conformation between two helix clusters in the center of the helical domain and is accessible through a hole in the surface of this domain. Dimensions and polarity of the hole match those of a Pyr <> Pyr dinucleotide, suggesting that the Pyr <> Pyr "flips out" of the helix to fit into this hole, and that electron transfer between the flavin and the Pyr <> Pyr occurs over van der Waals contact distance.

    Topics: Amino Acid Sequence; Computer Graphics; Crystallography, X-Ray; Deoxyribodipyrimidine Photo-Lyase; DNA Damage; DNA Repair; DNA, Bacterial; Electron Transport; Escherichia coli; Flavin-Adenine Dinucleotide; Folic Acid; Hydrogen Bonding; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Pyrimidine Dimers

1995
Absolute action spectrum of E-FADH2 and E-FADH2-MTHF forms of Escherichia coli DNA photolyase.
    Biochemistry, 1990, Aug-21, Volume: 29, Issue:33

    Escherichia coli DNA photolyase mediates photorepair of pyrimidine dimers occurring in UV-damaged DNA. The enzyme contains two chromophores, 1,5-dihydroflavin adenine dinucleotide (FADH2) and 5,10-methenyltetrahydrofolylpolyglutamate (MTHF). To define the roles of the two chromophores in the photochemical reaction(s) resulting in DNA repair and the effect of DNA structure on the photocatalytic step, we determined the absolute action spectra of the enzyme containing only FADH2 (E-FADH2) or both chromophores (E-FADH2-MTHF), with double- and single-stranded substrates and with substrates of different sequences in the immediate vicinity of the thymine dimer. We found that the shape of the action spectrum of E-FADH2 matches that of the absorption spectrum with a quantum yield phi (FADH2) = 0.69. The action spectrum of E-FADH2-MTHF is also in a fairly good agreement with the absorption spectrum with phi (FADH2-MTHF) = 0.59. From these values and from the previously established properties of the two chromophores, we propose that MTHF transfers energy to FADH2 with a quantum yield of phi epsilon T = 0.8 and that 1FADH2 singlet transfers an electron to or from the dimer with a quantum yield phi ET = 0.69. The chemical nature of the chromophores did not change after several catalytic cycles. The enzyme repaired a thymine dimer in five different sequence contexts with the same efficiency. Similarly, single- and double-stranded DNAs were repaired with the same overall quantum yield.

    Topics: Base Sequence; Deoxyribodipyrimidine Photo-Lyase; DNA Repair; Escherichia coli; Flavin-Adenine Dinucleotide; Folic Acid; Molecular Sequence Data; Protein Denaturation; Pyrimidine Dimers; Spectrophotometry, Ultraviolet; Substrate Specificity; Tetrahydrofolates

1990