flavin-adenine-dinucleotide and 2-aminobicyclo(2-2-1)heptane-2-carboxylic-acid

In perifused pancreatic islets, the fluorescence of oxidized flavoproteins (FAD) was recorded continuously. Elevation of glucose concentration in the medium form 0 or 5 mM to 20 mM led to decrease in FAD-fluorescence beginning 10 sec after change of medium. L-leucine (10 mM), (+/-)-B-BCH (20 mM) and alpha-ketoisocaproic acid (10 mM) caused typical kinetics of FAD-fluorescence decrease. The results are interpreted to indicate rapid changes of the functional state of B-cell mitochondria induced by the above-mentioned stimulators of insulin release.

Topics: Amino Acids; Amino Acids, Cyclic; Animals; Arginine; Caproates; Female; Flavin-Adenine Dinucleotide; Glucose; Islets of Langerhans; Keto Acids; Leucine; Male; Mice; Mice, Obese; Microscopy, Fluorescence; Mitochondria; NAD; NADP; Norbornanes; Oxidation-Reduction; Pentobarbital