flavin-adenine-dinucleotide has been researched along with 2-3-dimethoxy-5-methyl-6-decyl-1-4-benzoquinone* in 1 studies
1 other study(ies) available for flavin-adenine-dinucleotide and 2-3-dimethoxy-5-methyl-6-decyl-1-4-benzoquinone
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Steady-state kinetics and inhibitory action of antitubercular phenothiazines on mycobacterium tuberculosis type-II NADH-menaquinone oxidoreductase (NDH-2).
Type-II NADH-menaquinone oxidoreductase (NDH-2) is an essential respiratory enzyme of the pathogenic bacterium Mycobacterium tuberculosis (Mtb) that plays a pivotal role in its growth. In the present study, we expressed and purified highly active Mtb NDH-2 using a Mycobacterium smegmatis expression system, and the steady-state kinetics and inhibitory actions of phenothiazines were characterized. Purified NDH-2 contains a non-covalently bound flavin adenine dinucleotide cofactor and oxidizes NADH with quinones but does not react with either NADPH or oxygen. Ubiquinone-2 (Q2) and decylubiquinone showed high electron-accepting activity, and the steady-state kinetics and the NADH-Q2 oxidoreductase reaction were found to operate by a ping-pong reaction mechanism. Phenothiazine analogues, trifluoperazine, Compound 1, and Compound 2 inhibit the NADH-Q2 reductase activity with IC50 = 12, 11, and 13 microm, respectively. Trifluoperazine inhibition is non-competitive for NADH, whereas the inhibition kinetics is found to be uncompetitive in terms of Q2. Topics: Antitubercular Agents; Binding, Competitive; Enzyme Inhibitors; Flavin-Adenine Dinucleotide; Kinetics; Mycobacterium tuberculosis; NAD; Phenothiazines; Quinone Reductases; Quinones; Ubiquinone | 2006 |