ferrihydrite and ferrous-phosphate

Mineral transformations by two hyperthermophilic Fe(III)-reducing crenarchaea,

Topics: Ferric Compounds; Ferrosoferric Oxide; Iron; Minerals; Oxidation-Reduction

The effects of humic acids (HA) and fulvic acids (FA) on the fate of Cd in anaerobic environment upon microbial reduction of Cd-bearing ferrihydrite (Fh) with Geobacter metallireducens were investigated. The results showed that HA and FA could promote the reductive dissolution of Fh and the formation of vivianite. After incubation of 38 d, vivianite accounted for 47.19%, 59.22%, and 48.53% of total Fe in biological control batch (BCK), HA and FA batches (C/Fe molar ratio of 1.0), respectively, by Mössbauer spectroscopy analysis. In terms of Cd, HA and FA could promote the release of adsorbed Cd during the initial bioreduction process, but reassuringly, after 38 d the dissolved Cd with HA and FA addition batches were 0.58-0.91 and 0.99-1.08 times of the BCK, respectively. The proportions of residual Cd in HA batches were higher than FA and BCK batches, indicating that HA was better than FA in immobilizing Cd. This might be because the quinone groups in HA could act as electron shuttle. This study showed that HA facilitated the transformation of vivianite better than FA, and Cd can be stabilized by resorption or co-precipitation with vivianite, providing a theoretical support for the translocation of Cd in sediment-water interface.

Topics: Cadmium; Ferric Compounds; Humic Substances; Iron

Although PO

Topics: Arsenic; Bacteria; Ferric Compounds; Iron; Oxidation-Reduction; Oxides; Phosphates; Sulfates; Sulfur Oxides

Although iron- and sulfate-reducing bacteria in subsurface environments have crucial roles in biogeochemical cycling of C, Fe, and S, how specific electron donors impact the compositional structure and activity of native iron- and/or sulfate-reducing communities is largely unknown. To understand this better, we created bicarbonate-buffered batch systems in duplicate with three different electron donors (acetate, lactate, or glucose) paired with ferrihydrite and sulfate as the electron acceptors and inoculated them with subsurface sediment as the microbial inoculum. Sulfate and ferrihydrite reduction occurred simultaneously and were faster with lactate than with acetate. 16S rRNA-based sequence analysis of the communities over time revealed that Desulfotomaculum was the major driver for sulfate reduction coupled with propionate oxidation in lactate-amended incubations. The reduction of sulfate resulted in sulfide production and subsequent abiotic reduction of ferrihydrite. In contrast, glucose promoted faster reduction of ferrihydrite, but without reduction of sulfate. Interestingly, the glucose-amended incubations led to two different biogeochemical trajectories among replicate bottles that resulted in distinct coloration (white and brown). The two outcomes in geochemical evolution might be due to the stochastic evolution of the microbial communities or subtle differences in the initial composition of the fermenting microbial community and its development via the use of different glucose fermentation pathways available within the community. Synchrotron-based x-ray analysis indicated that siderite and amorphous Fe(II) were formed in the replicate bottles with glucose, while ferrous sulfide and vivianite were formed with lactate or acetate. These data sets reveal that use of different C utilization pathways projects significant changes in microbial community composition over time that uniquely impact both the geochemistry and mineralogy of subsurface environments.

Topics: Acetic Acid; Base Sequence; Biodegradation, Environmental; Carbon; Carbonates; Desulfotomaculum; DNA, Bacterial; DNA, Ribosomal; Electrons; Energy Metabolism; Ferric Compounds; Ferrous Compounds; Glucose; Lactic Acid; Metabolic Networks and Pathways; Microbial Consortia; Oxidation-Reduction; Phosphates; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sulfates

The dissolution of arsenic-bearing iron(III) (oxyhydr)oxides during combined microbial iron(III) and arsenate(V) reduction is thought to be the main mechanism responsible for arsenic mobilization in reducing environments. Besides its mobilization during bioreduction, arsenic is often resequestered by newly forming secondary iron(II)-bearing mineral phases. In phosphate-bearing environments, iron(II) inputs generally lead to vivianite precipitation. In fact, in a previous study we observed that during bioreduction of arsenate(V)-bearing biogenic iron(III) (oxyhydr)oxides in phosphate-containing growth media, arsenate(V) was immobilized by the newly forming secondary iron(II) and iron(II)/iron(III)mineral phases, including vivianite. In the present study, changes in arsenic redox state and binding environment in these experiments were analyzed. We found that arsenate(V) partly replaced phosphate in vivianite, thus forming a vivianite-symplesite solid solution identified as Fe3(PO4)1.7(AsO4)0.3·8H2O. Our data suggests that in order to predict the fate of arsenic during the bioreduction of abiogenic and biogenic iron(III) (oxyhydr)oxides in arsenic-contaminated environments, the formation of symplesite-vivianite minerals needs to be considered. Indeed, such mineral phases could contribute to a delayed and slow release of arsenic in phosphate-bearing surface and groundwater environments.

Topics: Arsenates; Arsenic; Ferric Compounds; Ferrous Compounds; Groundwater; Iron; Oxidation-Reduction; Phosphates; Shewanella; X-Ray Absorption Spectroscopy