ex-527 and entinostat

Histone deacetylase inhibitors (HDACi) are used as therapeutics for several B cell-derived malignancies. Furthermore, they have been shown to modulate the response of the immune system, like the B cell function. HDACi treatment affects differentiation, proliferation, and survival of B cells. Here we describe how to investigate the effects of HDACi treatment on naïve B cells regarding class-switch recombination (CSR) in vitro using flow cytometry.

Topics: Animals; B-Lymphocytes; Benzamides; Carbazoles; Cell Cycle; Cell Differentiation; Cell Proliferation; Cell Survival; Cytokines; Flow Cytometry; Histone Deacetylase Inhibitors; Histone Deacetylases; Hydroxamic Acids; Immunoglobulin Class Switching; Immunoglobulins; Immunomagnetic Separation; Indoles; Lipopolysaccharides; Mice; Mice, Inbred C57BL; Panobinostat; Primary Cell Culture; Pyridines; Recombination, Genetic