euscaphic-acid and corosolic-acid

This study was designed to investigate triterpenoids from the roots of Rosa laevigata Michx.\ The silica gel column chromatography was used to separate the chemical constituents from the roots of Rosa\ laevigata Michx. HPLC was used to analyze its purity and chemical constitution. Spectroscopy methods were\ used to determine their structures. Five constituents were isolated and identified as19α-OH-3β-E-feruloyl corosolic\ acid (1), 23-hydroxy-tormentic acid (2), 2α, 3β, 19α, 23- tetrahydroxy-12-en-28-oleanolic acid (3), 2α, 3α, 20β-\ trihydroxyurs-13 (18)-en-28-oic-acid (4), 2α, 3β, 20β-trihydroxyurs-13 (18)-en-28-oic-acid (5). Compound 1 was\ assigned as a new compound, compounds 4, 5 were obtained from the genus Rosa for the first time.

Topics: Chromatography, High Pressure Liquid; Molecular Structure; Oleanolic Acid; Plant Extracts; Plant Roots; Rosa; Triterpenes

To study the chemical constituents of Rubus stans.. The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures were identified on the basis of spectroscopic analysis and physicochemical properties.. Eleven compounds were isolated and purified from the ethanol extract of Rubus stans. They were identified as β-sitosterol( 1),betulinic acid( 2),euscaphic acid( 3),ursolic acid( 4),corosolic acid( 5),kaempferol( 6),quercetin( 7),2α,3β,19α,23-tetrahydroxy-urs-12-en-28-oic acid( 8),β-daucosterol( 9),quercetin-3-O-β-D-glucoside( 10) and kaempferol-3-O-β-D-6-O-( p-hydroxycinnamoyl)-glucopyranoside( 11).. All the compounds are isolated from this plant for the first time.

Topics: Betulinic Acid; Kaempferols; Pentacyclic Triterpenes; Rubus; Sitosterols; Triterpenes; Ursolic Acid

Corosolic acid (CRA), maslinic acid (MA), and tormentic acid (TA) are three kind of bioactive constituents of Eriobotrta japonica leaves. In this study, plasma protein binding model prediction suggested that the binding ability to HSA was CRA>MA>TA. Furthermore, fluorescence spectroscopy confirmed this prediction. The results from emission and time resolved fluorescence studies revealed that the emission quenching of HSA with CRA, MA, and TA were all initiated by static quenching mechanism. From molecular docking results and site marker competitive experimental results it was possible to make good estimates about CRA, MA, and TA mainly bound to subdomain IIA of HSA. 3D fluorescence, FT-IR and CD spectra indicated that the local conformation of HSA molecules was affected by the presence of CRA, MA, and TA, but at different extents.

Topics: Binding Sites; Circular Dichroism; Eriobotrya; Humans; Molecular Docking Simulation; Plant Leaves; Protein Binding; Protein Structure, Tertiary; Serum Albumin; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Triterpenes

Potentilla discolor Bunge has been used for diabetes in China for a long time. Corosolic acid (CA) and euscaphic acid (EA), with significant anti-diabetic activity, are two major triterpenoids in P. discolor. In this study, a specific, sensitive and convenient LC-MS method has been developed for simultaneous determination of CA and EA in the plasma of normal and diabetic rats after oral administration of the extract of P. discolor. The chromatographic separation was achieved using an Alltima C18 column (53 × 7.0 mm, i.d., 3 µm) with a mobile phase composed of 0.1% formic acid water and 0.1% formic acid acetonitrile at a flow rate of 1.0 mL/min. The detection was performed by MS with electrospray ionization interface in negative selected ion monitoring mode. All the validation data, such as specificity, linearity (r(2)  > 0.9991 within 0.025-10.0 µg/mL), lower limit of quantitation (2.5 ng/mL), precision (intra- and inter-day <14.7%), accuracy (<15.0%), recovery (85.7-110.8%) and stability were determined and all of them were within the required limits. This method was successfully applied for the evaluation of the pharmacokinetic behaviors of these two compounds in the plasma of normal and diabetic rats.

Topics: Administration, Oral; Animals; Chromatography, High Pressure Liquid; Diabetes Mellitus, Experimental; Drugs, Chinese Herbal; Humans; Male; Potentilla; Rats; Rats, Sprague-Dawley; Spectrometry, Mass, Electrospray Ionization; Triterpenes

Triterpenoids of ursane class: ursolic acid, ilelatifol D, corosolic acid and euscaphic acid were isolated for the first time from Leonurus cardiaca, a member of the family Lamiaceae. The isolated compounds were tested for their cell-based antiinflammatory potential by suppressing respiratory burst activity and superoxide scavenging property by using xanthine/xanthine oxidase system to produce superoxides in the cell-free system. Ursolic acid was found to be an excellent inhibitor for the superoxides produced in the cellular system, while the same was inactive in the superoxide scavenging activity in cell-free system.

Topics: Cells, Cultured; Dose-Response Relationship, Drug; Free Radical Scavengers; Humans; Lamiaceae; Molecular Structure; Neutrophils; Respiratory Burst; Superoxides; Triterpenes; Ursolic Acid; Xanthine Oxidase

A new 27-carboxylic lupane-type triterpene, tiarellic acid (1), was isolated from Tiarella polyphylla together with corosolic acid (2) and tormentic acid (3). Tiarellic acid was characterized as 3,23-dihydroxy-20(29)-lupen-27-oic acid and its NMR data were unambiguously assigned using 2-D NMR techniques.

Topics: Antineoplastic Agents, Phytogenic; Korea; Magnetic Resonance Spectroscopy; Molecular Conformation; Plants, Medicinal; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Infrared; Triterpenes

Five triterpenoid compounds were isolated from Rubus alceaefollius. On the basis of the chemical and spectral analysis the compounds were identified as corosolic acid, tormentic acid, niga-inchigoside F1, trachelosperoside E-1 and suavissimoside R1, were obtained from the plant for the first time.

Topics: Drugs, Chinese Herbal; Molecular Structure; Plants, Medicinal; Rosales; Triterpenes