europium-cryptate has been researched along with deoxyuridine-triphosphate* in 1 studies
1 other study(ies) available for europium-cryptate and deoxyuridine-triphosphate
| Article | Year |
|---|---|
Europium cryptate labeled deoxyuridine-triphosphate analog: synthesis and enzymatic incorporation.
The synthesis of an europium tris-bipyridine cryptate labeled 2'-deoxyuridine-5 '-triphosphate analog (K-11-dUTP) is described. This labeled triphosphate was incorporated into DNA through enzymatic reactions with terminal transferase and DNA polymerases. The enzymatic reactions were monitored by TRACE (Time Resolved Amplification of Cryptate Emission), a homogeneous method using Fluorescence Resonance Energy Transfer (FRET) from an europium cryptate as donor to a modified allophycocyanine as acceptor. Topics: Deoxyuracil Nucleotides; Deoxyuridine; DNA; DNA Nucleotidylexotransferase; Energy Transfer; Fluorescence; Fluorescent Dyes; Humans; Kinetics; Molecular Structure; Oligodeoxyribonucleotides; Organometallic Compounds | 2000 |