europium-cryptate has been researched along with 4-azidophenylalanine* in 1 studies
1 other study(ies) available for europium-cryptate and 4-azidophenylalanine
| Article | Year |
|---|---|
Bioorthogonal Labeling of Ghrelin Receptor to Facilitate Studies of Ligand-Dependent Conformational Dynamics.
Ghrelin receptor (GhrR) is a promising drug target because of its central role in energy homeostasis. GhrR, known for high constitutive activity, is thought to display multi-state conformations during activation and signaling. We used genetically encoded unnatural amino acids and bioorthogonal labeling reactions to engineer multiple fluorescent donor-acceptor pairs to probe ligand-directed structural changes in GhrR. We demonstrate how conformational dynamics of a G-protein-coupled receptor can be measured in reconstituted systems. Topics: Antibodies, Monoclonal; Azides; Cycloaddition Reaction; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Humans; Ligands; Organometallic Compounds; Phenylalanine; Protein Conformation; Receptors, Ghrelin | 2015 |