ethylmaleimide has been researched along with cysteine in 654 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 260 (39.76) | 18.7374 |
| 1990's | 206 (31.50) | 18.2507 |
| 2000's | 141 (21.56) | 29.6817 |
| 2010's | 42 (6.42) | 24.3611 |
| 2020's | 5 (0.76) | 2.80 |
| Authors | Studies |
|---|---|
| Keil, B; Keil-Dlouha, V; Lecroisey, A; Perrin, D; Woods, DR | 1 |
| Danpure, CJ | 1 |
| Craven, PA; DeRubertis, FR | 1 |
| Bauer, H; Kresze, GB; Lynen, F; Oesterhelt, D; Steber, L | 1 |
| Iwakura, M; Katsuki, H; Tokushige, M | 1 |
| Junikiewicz, E; Szydlowska, H | 2 |
| Horigome, T; Yamashita, T | 1 |
| Porter, M; Weber, A | 1 |
| Brevet, A; Pradel, LA; Zeitoun, Y | 1 |
| Danpure, CJ; Fyfe, DA | 1 |
| Vas, M | 1 |
| Bartha, F; Vas, M | 1 |
| Demain, AL; Inamine, E | 1 |
| Gregory, EM | 1 |
| Boettcher, B; Martinez-Carrion, M | 1 |
| Abe, Y; Aomine, M | 1 |
| Goldberg, ME; Raibaud, O | 1 |
| Davidson, BE; Gething, MJ | 1 |
| Manly, SP; Matthews, KS | 1 |
| Brown, RD; Matthews, KS | 1 |
| Galivan, J; Maley, F; Noonan, J | 1 |
| Doonan, S; Marra, E; Passarella, S; Quagliariello, E; Saccone, C | 1 |
| Feeney, RE; Nashef, AS; Osuga, DT | 1 |
| Drabikowski, W; Gergely, J; Lehrer, S; Nagy, B | 1 |
| Portanova, JP; Shrift, A | 1 |
| Hayakari, M; Izumi, H; Ozawa, H | 1 |
| Hayakari, M; Izumi, H; Oyama, H; Ozawa, H | 1 |
| Hayakari, M; Izumi, H; Oyama, H; Ozawa, K | 1 |
| Baliga, BS; Munro, HN; Nelson, E | 1 |
| Lagunoff, D | 1 |
| Berry, RW; Weisblat, DA | 1 |
| Kochkina, VM; Torchinskii, YM | 1 |
| Chignard, M; Tranier, Y; Vargaftig, BB | 1 |
| Heinle, H; Wendel, A; Wiest, E | 1 |
| Crichton, RR; Wetz, K | 1 |
| Bowie, L; Crawhall, JC; Gochman, N; Johnson, K; Schneider, JA | 1 |
| Gee, N; Gore, MG; Greasley, PJ; Knowles, MR; McAllister, G; Ragan, CI | 1 |
| Howley, PM; Klausner, RD; McBride, AA | 1 |
| Gee, NS; Gore, MG; Greasley, PJ; Knowles, MR; McAllister, G; Ragan, CI | 1 |
| Felton, JR; Hoover, CI; Ng, CY | 1 |
| Pleckaityte, M; Siksnys, V | 1 |
| Lee, RS; Senior, AE; Wilke-Mounts, S | 1 |
| Feng, Y; Forgac, M | 3 |
| Cartoux, L; Chase, PB; Chen, T; DasGupta, G; Kushmerick, MJ; Reisler, E | 1 |
| Altman, RA; Gonzalez-DeWhitt, PA; Greenberg, BD; Kisilevsky, R; Lowery, DE; Narindrasorasak, S | 1 |
| DeMartino, GN; Dick, LR; Moomaw, CR; Pramanik, BC; Slaughter, CA | 1 |
| Aggeler, R; Cai, SX; Capaldi, RA; Chicas-Cruz, K; Keana, JF | 1 |
| Hochstein, LI; Stan-Lotter, H | 1 |
| Colman, RF; Smyth, GE | 2 |
| Hecker, M; Macarthur, H; Sessa, WC; Siegle, I; Vane, JR | 1 |
| Cheon, HG; Hanna, PE | 1 |
| Bhaumik, D; Datta, AK | 1 |
| Mansoor, MA; Svardal, AM; Ueland, PM | 1 |
| Hartman, FC; Milanez, S; Mural, RJ | 1 |
| Ahn, HS; Foster, C; Foster, M; Sybertz, E; Wells, JN | 1 |
| al Jamal, JA; Benz, R; De Pinto, V; Genchi, G; Palmieri, F | 1 |
| Norton, TR; Ringer, DP; Self, RR | 1 |
| Brennan, L; Ferenci, T; Francis, G | 1 |
| Fujioka, M; Konishi, K | 1 |
| Hatayama, I; Oikawa, A; Sato, K; Satoh, K; Shen, HX; Tamai, K; Tsuchida, S; Yasui, A | 1 |
| Colman, RW; Puri, RN | 1 |
| Charles, H; Simms, SA; Subbaramaiah, K | 1 |
| Haapasalo, M; McBride, BC; Singh, U; Uitto, VJ | 1 |
| Phelps, A; Wohlrab, H | 1 |
| Martin Neto, L; Nascimento, OR; Tabak, M | 1 |
| Corrales, FJ; Mato, JM; Ochoa, P; Pajares, MA | 1 |
| Chen, VJ; Frolik, CA; Hassell, TC; Holbrook, NL; Johnson, MG; Kriauciunas, A; Skatrud, PL | 1 |
| Brüne, B; Lapetina, EG | 1 |
| Agarwal, R; Burke, M; Mayadevi, M; Rajasekharan, KN | 1 |
| Angleton, EL; Birkedal-Hansen, H; Springman, EB; Van Wart, HE | 1 |
| Dong, Q; Fromm, HJ; Liu, F; Soans, C | 1 |
| Imai, S; Kitamura, Y; Matsueda, R; Nomura, Y | 1 |
| Everett, EA; Falick, AM; Reich, NO | 1 |
| Ikehara, M; Kanaya, S; Katsuda, C; Kimura, S | 1 |
| Aschner, M; Eberle, NB; Goderie, S; Kimelberg, HK | 1 |
| Detwiler, TC; Speziale, MV | 1 |
| Bukusoglu, C; Guérin, B; Rakotomanana, F; Wohlrab, H | 1 |
| Brodie, AE; Reed, DJ | 1 |
| Hutchison, KA; Matić, G; Meshinchi, S; Pratt, WB | 1 |
| Salo, T; Tryggvason, K; Turpeenniemi-Hujanen, T | 1 |
| Gaspar, AR; Neitz, AW; Vermeulen, NM; Verschoor, JA | 1 |
| Ferrari, N; Pfeffer, U; Vidali, G | 1 |
| Bhatnagar, A; Das, B; Liu, SQ; Srivastava, SK | 1 |
| Benjouad, A; Desmadril, M; el Hachimi, Z; Tijane, M; Yon, JM | 1 |
| Pardo, JP; Slayman, CW | 1 |
| Burns, DL; Manclark, CR | 1 |
| Low, PS; Thevenin, BJ; Willardson, BM | 1 |
| Lieberman, DM; Reithmeier, RA; Werner, PK | 1 |
| Bednar, RA; Fried, WB; Lock, YW; Pramanik, B | 1 |
| Heese, D; Röhm, KH | 1 |
| Hatefi, Y; Yamaguchi, M | 1 |
| Biessen, EA; Horn, AS; Norder, JA; Robillard, GT | 1 |
| Bannai, S | 1 |
| Ahmad, S; Aljian, J; Okine, L; Vistica, DT; Wood, R | 1 |
| Baba, A; Iwata, H; Nishiuchi, Y; Tatsuno, T; Uemura, A | 1 |
| Fisher, SE; Karl, PI; Tkaczevski, H | 1 |
| Fahey, RC; Fenton, SS | 1 |
| Kawakita, M; Yamashita, T | 1 |
| Hartman, FC; Omnaas, J; Porter, MA | 1 |
| Hubert, E; Reyes, A; Slebe, JC | 2 |
| Antholine, WE; Manoharan, PT; Rifkind, JM; Taketa, F; Wang, JT | 1 |
| Brown, SG; Han, PF; Pearson, KH; Zeidan, HM | 1 |
| Nicholls, DG; Rial, E | 1 |
| Köhler, H; Neupert, W; Nicholson, DW | 1 |
| Burgos, ME; Hubert, E; Reyes, A; Slebe, JC | 1 |
| Kato, H; Kimura, A; Nishioka, T; Oda, J; Tanaka, T | 1 |
| Dernburg, AF; Falke, JJ; Koshland, DE; Milligan, DL; Sternberg, DA; Zalkin, N | 1 |
| Anraku, Y; Yamato, I | 1 |
| Kaback, HR; Menick, DR; Poonian, MS; Sarkar, HK; Viitanen, PV | 1 |
| Barden, JA; dos Remedios, CG; Hambly, BD; Miki, M | 1 |
| Bradley, JD; Neer, EJ; Smith, JA; Winslow, JW | 1 |
| Gershoni, JM; Mosckovitz, R | 1 |
| Anderson, PM; Fuchs, JA; Johnson, WV; Korte, JJ; Sung, YC; Xiong, XF | 1 |
| Kretsinger, RH; Persechini, A | 1 |
| Pas, HH; Robillard, GT | 1 |
| Alberta, JA; Fisher, RR; Modrak, DE; Wu, LN | 1 |
| Tu, GC; Weiner, H | 1 |
| Bieseler, B; Ehring, R; Stochaj, U | 1 |
| Brooker, RJ; Wilson, TH | 1 |
| Profy, AT; Schimmel, P | 1 |
| Hackett, CS; Novoa, WB; Ozols, J; Strittmatter, P | 1 |
| Clarke, JH; Martinez-Carrion, M | 1 |
| Barden, JA; dos Remedios, CG; Miki, M | 1 |
| Beyreuther, K; Bieseler, B; Prinz, H | 1 |
| Kaback, HR; Menick, DR; Sarkar, HK; Trumble, WR; Viitanen, PV | 1 |
| Lolkema, JS; Robillard, GT; ten Hoeve-Duurkens, RH | 1 |
| King, MM | 1 |
| Ikeda, Y; Okamura-Ikeda, K; Tanaka, K | 1 |
| Caughey, WS; Kawanishi, S | 1 |
| Deziel, MR; Jung, CY; Rothstein, A | 1 |
| Kolbe, HV; Wohlrab, H | 1 |
| Gluckman, MI; Triester, S; Yu, DH | 1 |
| Roediger, WE | 1 |
| Apffel, CA; Nathanson, IT; Walker, JE | 1 |
| Chapman, JD; Greenstock, CL; Raleigh, JA; Reuvers, AP; Shiermen, E | 1 |
| Hegner, D | 1 |
| Oe, M; Seon, BK; Titani, K; Tonomura, Y | 1 |
| Hasselbach, W; Taugner, G | 1 |
| Farron, F; Racker, E | 1 |
| Feddersen, C; Gormsen, J | 1 |
| Braun, HE; Mondt, H; Towliati, H; Weber, E | 1 |
| Holmsen, H; Holmsen, I | 1 |
| McHenry, CS; Santi, DV | 1 |
| Blum, JJ; Hayes, A | 1 |
| Anderson, BM; Anderson, CD; Heitz, JR | 1 |
| Foor, WE; Huggins, CG; Hurst, MW; Tou, JS | 1 |
| Hamilton, IR; Khandelwal, RL | 1 |
| Farcasiu, M; Istratoiu, R; Milvy, P | 1 |
| Gregory, EM; Harrison, JH; Rohrbach, MS; Yost, FJ | 1 |
| Pittman, JA; Starnes, WR | 1 |
| Iwata, H; Muraki, K; Yamamoto, I | 1 |
| Nakamura, M; Yamazaki, I | 1 |
| Fleisch, JH; Krzan, MC; Titus, E | 1 |
| Bentley, PJ | 1 |
| Hartzell, RW; Neurath, AR; Rubin, BA; Vernon, SK | 1 |
| Goitein, RD; Piszkiewicz, D | 1 |
| Catt, KJ; Dufau, ML; Ryan, D | 1 |
| Fan, CC; Plaut, GW | 1 |
| Degani, Y; Nyc, JF; Smith, EL; Veronese, FM | 1 |
| Cantagalli, P; Dianzani, F; Gagnoni, G; Grasso, A | 2 |
| Wills, ED | 2 |
| Chu, R; Colman, RF | 1 |
| Holland, P; Little, C | 1 |
| Kitano, T; Murano, T; Nishino, H; Yamamoto, H | 1 |
| Murano, T; Okada, N; Yamamoto, H; Yano, I | 1 |
| Sonenberg, N; Wilchek, M; Zamir, A | 1 |
| Giordano, GF; Lichtman, MA | 1 |
| Kula, MR | 1 |
| Drobnica, L; Ondrejicková, O; Rychlík, I; Sedlácek, J | 1 |
| Titani, Y; Tsuruta, Y | 1 |
| Birchmeier, W; Christen, P; Wilson, KJ | 2 |
| Gladner, JA; Klee, CB | 1 |
| Fenselau, A; Moore, J | 1 |
| Kitano, T | 1 |
| Butler, M; Giannina, T; Steinetz, B | 1 |
| Arvidsson, L; Larsson-Raźnikiewicz, M | 1 |
| Harris, JI; Nicolas, JC | 1 |
| Greenberg, J; Johnson, BF | 1 |
| Birchmeier, W; Christen, P; Zaoralek, PE | 1 |
| Kanatani, H; Kishimoto, T | 1 |
| Planas-Bohne, F | 1 |
| Brubacher, LJ; Glick, BR | 1 |
| Hastings, JW; Meighen, EA; Nicoli, MZ | 1 |
| Bocharov, AL; Demidkina, TV; Karpeiskii, MY; Polyanovskii, OL | 1 |
| Wheldrake, JF | 1 |
| Carter, JR; Fox, CF; Kennedy, EP | 1 |
| Ellis, RJ | 3 |
| Hardman, JK; Yanofsky, C | 2 |
| Hardman, JK; Malkinson, AM | 2 |
| Der Terrossian, E; Kassab, R; Pradel, LA; Thoai, NV | 1 |
| Brown, WP; Knox, J; Spero, L | 1 |
| Kanaoka, Y; Kimura, K; Machida, M; Watanabe, A | 1 |
| Cooperstein, SJ; Lazarow, A; Watkins, D | 1 |
| Fontana, A; Scoffone, E | 1 |
| Emmons, PR; Harrison, MJ; Mitchell, JR | 1 |
| Benckhuijsen, C | 1 |
| Iyer, GY; Paniker, NV | 1 |
| Egami, F; Ishii, S; Yui, C | 1 |
| Kornberg, A; Setlow, P | 1 |
| Bernheim, F | 1 |
| Horowitz, MI; Kim, YS | 1 |
| Bloemmen, J; Lontie, R | 1 |
| Carraway, KL; Shin, BC | 1 |
| Aoki, K; Saito, T | 1 |
| Felberg, NT; Hollocher, TC | 1 |
| Brauner, MJ; Majerus, PW; Minnich, V; Smith, MB | 1 |
| Mäkinen, KK; Mäkinen, PL | 1 |
| Desvages, G; Kassab, R; Pradel, LA | 1 |
| Tyler, HJ; Weitzman, PD | 1 |
| Kress, LF; Laskowski, M; Wilson, KA | 1 |
| Schneider, F; Wenck, H | 1 |
| Joshi, VC; Plate, CA; Wakil, SJ | 1 |
| Mapes, CJ | 1 |
| Barker, HA; Zappia, V | 1 |
| Kail, M; Schneider, F; Wenck, H | 1 |
| Krishnan, PS; Kumar, KS | 1 |
| Pradel, LA; Roustan, C | 1 |
| Chang, CC; Lorber, A; Masuoka, D; Meacham, I | 1 |
| Jákó, J; László, C; Pálos, LA; Sas, G | 1 |
| Smith, FM; Verpoorte, JA | 1 |
| Jocelyn, PC; Kamminga, A | 1 |
| Smith, IK; Thompson, JF | 1 |
| Martin, K | 1 |
| Giovanelli, J; Mudd, SH | 1 |
| Yamamoto, M | 1 |
| Guidotti, G; Konigsberg, W; Neer, EJ | 1 |
| Barns, RJ; Keech, DB | 1 |
| Jeanjean, R; Vallée, M | 1 |
| Mahadevan, S; Shukla, PS | 1 |
| Franklin, JG; Leslie, J | 1 |
| Mäkinen, KK | 1 |
| Evans, Z; McVeigh, I | 1 |
| Smyth, DG; Tuppy, H | 1 |
| Williams, VR; Wu, WT | 1 |
| Lüscher, EF; Mueller-Eckhardt, C | 1 |
| Entlicher, G; Kostír, J; Prosková, M; Tichá, M | 1 |
| Huovinen, JA | 1 |
| Falconi, C | 1 |
| Segal, S; States, B | 1 |
| De Barreiro, OL | 1 |
| Morgan, TE | 1 |
| Meister, A; Ronzio, RA; Rowe, WB; Wilk, S | 1 |
| Naylor, AW; Rosenthal, GA | 1 |
| Fasold, H; Lusty, CJ | 1 |
| Baker, E; Morgan, EH | 1 |
| Konishi, N; Toda, N | 1 |
| Blumenfeld, OO; Konigsberg, W; Smyth, DG | 1 |
| Damjanovich, S; Jókay, I; Tóth, S | 1 |
| Haugaard, N; Horn, RS | 1 |
| Flavin, M; Kaplan, MM | 1 |
| Cole, PW; Folk, JE | 1 |
| Segel, IH; Yamamoto, LA | 1 |
| Doughty, G; Gorin, G; Martic, PA | 1 |
| Alam, A; Arcos, JC; Argus, MF; Mathison, JH | 1 |
| Hidaka, H; Kuzuya, H; Nagatsu, T | 1 |
| Bradley, K; Schneider, JA; Seegmiller, JE | 1 |
| Edwards, JB; Keech, DB | 1 |
| DiBona, FJ; Lessard, JL; Smits, MR; Taketa, F | 1 |
| El-Maghrabi, MR; Pate, TM; Pilkis, J; Pilkis, SJ | 1 |
| Boulay, F; Vignais, PV | 1 |
| MacLennan, DH; Reithmeier, RA | 1 |
| Schmerold, I; Tempel, K; Winkle, J; Wulffius-Kock, M | 1 |
| Chao, ES; Frenkel, GD | 1 |
| Goette, A; Pfahler, W; Schmerold, I; Tempel, K | 1 |
| Bragg, PD; Stan-Lotter, H | 1 |
| Fullmer, CS; McCarty, RE; Moroney, JV | 1 |
| Huttunen, A; Lindy, S; Turto, H; Uitto, J; Uitto, VJ | 1 |
| Piette, LH; Watanabe, K; Yasunobu, KT; Zeidan, H | 1 |
| Fox, JM; Hof, D | 1 |
| Beintema, JJ; Jekel, PA; Müller, F; van Berkel, WJ; Weijer, WJ | 1 |
| Angelaccio, S; Barra, D; Bossa, F; Doonan, S; Martini, F | 1 |
| de Souza Otero, A; Hamilton, SL | 1 |
| Appaji Rao, N; Manohar, R | 1 |
| De Burlet, G; Linder, M; Sudaka, P | 1 |
| Bolen, JB; Israel, MA | 1 |
| Malm, B | 1 |
| Bull, P; Valenzuela, P; Wyneken, U | 1 |
| Juliao, SF; Regen, DM; Schraw, WP; Tarpley, HL | 1 |
| Asano, M; Hidaka, H | 1 |
| Subramanian, AR | 1 |
| Higgins, W; Miles, EW | 1 |
| Gennis, RB; Koland, JG | 1 |
| Bashan, N; Gahl, WA; Schulman, JD; Steinherz, R; Tietze, F | 1 |
| Bashan, N; Bernardini, I; Gahl, WA; Schulman, JD; Tietze, F | 1 |
| Evans, BL; Horton, HR; Knopp, JA | 1 |
| Clary, LG; Harrison, JH; Hodges, CT; Howell, SM; Wood, DC | 1 |
| Cutler, J; Haugaard, N; Ruggieri, MR | 1 |
| Bannai, S; Kitamura, E | 1 |
| Christen, P; Gehring, H | 1 |
| Bennett, N; Ildefonse, M; Serre, V | 1 |
| Henderson, BR; Hirling, H; Kühn, LC | 1 |
| Eckardt, S; Köhr, G; Lüddens, H; Monyer, H; Seeburg, PH | 1 |
| Goto, S; Hong, YM; Hori, H; Majima, E; Shinohara, Y; Terada, H | 1 |
| Clarke, DM; Loo, TW | 2 |
| Frillingos, S; Kaback, HR; Wu, J | 1 |
| Khorana, HG; Ridge, KD; Zhang, C | 1 |
| Schmidt, G; Senior, AE | 1 |
| Goldstein, J; Lunn, R; Suyama, K | 1 |
| Guerra, G; Pardo, JP; Uribe, S | 1 |
| Adachi, H; Aketa, M; Mizoguchi, T; Nanjo, H; Nishihara, T; Terada, T | 1 |
| Diaz, I; Garcia-Olmedo, F; Piñeiro, M; Rodriguez-Palenzuela, P; Titarenko, E | 1 |
| Aplin, RT; Gul, S; O'Grady, P; Roessner, CA; Scott, AI; Shoolingin-Jordan, PM; Warren, MJ | 1 |
| Bird, M; Britten, C; Hood, C | 1 |
| Hong, YM; Koike, H; Majima, E; Shinohara, Y; Terada, H | 1 |
| Inagaki, Y; Kimura, T; Sawai, T; Yamaguchi, A | 1 |
| Lei, Y; Liu, HW; Ploux, O; Vatanen, K | 1 |
| Gomi, S; Homma, M; Imae, Y; Iwama, T; Kawagishi, I | 1 |
| Frillingos, S; Kaback, HR; Voss, J; Wu, J | 1 |
| Bibi, E; Frillingos, S; Gonzalez, A; Kaback, HR; Sahin-Tóth, M | 1 |
| Shields, D; Xu, H | 1 |
| Kim, EJ; Rea, PA; Zhen, RG | 1 |
| Block, MA; Douce, R; Joyard, J; Maréchal, E; Miège, C | 1 |
| Maggert, K; Morgan, L; Nelson, H; Nelson, N; Rubinstein, B; Taiz, L; Taiz, SL; Yatabe, B | 1 |
| Jung, H; Jung, K; Kaback, HR | 1 |
| Futai, M; Iwamoto, A; Maeda, M; Orita-Saita, Y | 1 |
| Kimura, T; Sawai, T; Yamaguchi, A | 2 |
| Bandyopadhyay, S; Gronostajski, RM | 1 |
| Jordan, PM; Spencer, P | 1 |
| Mashhoon, N; Reich, NO | 1 |
| Cohan, P; Kaback, HR; Persson, B; Sahin-Tóth, M; Schwieger, J | 1 |
| Kanazawa, T; Nakamura, S; Suzuki, H | 1 |
| Gillespie, JS; Liu, X; Martin, W | 1 |
| McCarty, RE; Ong, AM; Soteropoulos, P | 1 |
| Bovee-Geurts, PH; DeGrip, WJ; Rath, P; Rothschild, KJ | 1 |
| Hagiwara, Y; Hayashi, K; Mizuno, Y; Ozawa, E; Suzuki, A; Yoshida, M | 1 |
| Choi, EJ; Dittman, AH; Duhe, RJ; Nielsen, MD; Storm, DR; Villacres, EC | 1 |
| Fonda, ML; Gao, G | 1 |
| Futai, M; Maeda, M; Omote, H; Park, MY | 1 |
| Blume, AJ; Brown, AM; Dushin, RG; George, SM; Jacobsen, JS; Sonnenberg-Reines, J | 1 |
| Ali, MS; Patel, MS; Roche, TE | 1 |
| Kachar, B; Kalinec, F | 1 |
| Dunten, RL; Kaback, HR; Sahin-Tóth, M | 1 |
| Blau, N; Bürgisser, DM; Heizmann, CW; Hunziker, P; Redweik, U; Thöny, B | 1 |
| Kaback, HR; Sahin-Tóth, M | 2 |
| Dumont, S; Fournel, M; Veillette, A | 1 |
| Haile, D; Klausner, RD; Philpott, CC; Rouault, TA | 1 |
| Jonsson, CB; Roth, MJ | 1 |
| Freedman, RB; Lennarz, WJ; Noiva, R | 1 |
| Bravo, N; Iriarte, A; Ludwig, H; Reyes, AM; Slebe, JC | 1 |
| Cotgreave, IC; Moldéus, P; Moore, GA; Norbeck, K; Weis, M | 1 |
| Fucile, NW; Sheflin, LG; Spaulding, SW | 1 |
| Aggeler, R; Capaldi, RA; Lee, RS; Senior, AE; Turina, P | 1 |
| Cash, VL; Dean, DR; Jack, RF; White, RH; Zheng, L | 1 |
| al-Nassar, KE; Fatania, HR; Thomas, N | 1 |
| Ash, DE; Werneburg, BG | 1 |
| Faulstich, H; Heintz, D | 1 |
| Hotta, K; Ichikawa, T; Ohara, S; Saigenji, K; Watanabe, T | 1 |
| Frillingos, S; Kaback, HR | 2 |
| Milner, JA; Sundaram, SG | 1 |
| Rao, NA; Santha, R; Vaidyanathan, CS | 1 |
| Capaldi, RA; Tang, C | 1 |
| Earm, YE; Han, J; Ho, WK; Kim, E | 1 |
| Connolly, DT; Glenn, K; Heuvelman, D | 1 |
| Barber, MJ; Quinn, GB; Smith, ET; Trimboli, AJ | 1 |
| Hirai, H; Hirano, N; Kurokawa, M; Mitani, K; Ogawa, S; Tanaka, K; Tanaka, T; Yazaki, Y | 1 |
| Colman, RW; Ghazaleh, FA; Omburo, GA | 1 |
| Bigelow, DJ; Chen, LT; Negash, S; Squier, TC | 1 |
| Ameyibor, E; Fu, W; Gardet-Salvi, L; Johnson, MK; Knaff, DB; Schürmann, P; Staples, CR; Stritt-Etter, AL | 1 |
| Liu, R; Sharom, FJ | 1 |
| Bates, JN; Holmes, RW; Snyder, GD; Van Voorhis, BJ | 1 |
| Chinenov, Y; Martin, ME; Schmidt, TK; Yang, XY; Yu, M | 1 |
| Hubbell, WL; Kaback, HR; Voss, J; Wu, J | 1 |
| Capaldi, RA; Haughton, MA | 1 |
| Farrens, DL; Hubbell, WL; Khorana, HG; Yang, K | 1 |
| Chew, A; Isaya, G; Rollins, RA; Sakati, WR | 1 |
| Harris, C; Hiranruengchok, R | 1 |
| He, MM; Kaback, HR; Sun, J | 1 |
| Dost, MH; Kok, J; Venema, G; Venema, K | 1 |
| Brewer, JW; Corley, RB | 1 |
| Kim, E; Reisler, E | 1 |
| Casey, PJ; Fu, HW; Moomaw, CR; Moomaw, JF | 1 |
| Keng, YF; Viola, RE | 1 |
| Landy, A; Pargellis, CA; Tirumalai, RS | 1 |
| Beyer, K; Nuscher, B | 1 |
| Kimura, T; Sawai, T; Suzuki, M; Yamaguchi, A | 1 |
| Franklin, MR; Kushner, JP; Phillips, JD; Sowa, AM; Wyckoff, EE | 1 |
| Lipton, SA; Pan, ZH; Segal, MM | 1 |
| Frillingos, S; Gonzalez, A; Kaback, HR; Sun, J | 1 |
| Kimura, T; Ohnuma, M; Sawai, T; Yamaguchi, A | 1 |
| Gabriel, JL; Pieringer, RA; Soprano, DR; Soprano, KJ; Wolfgang, CL; Zhang, Z | 1 |
| Pardo, JP; Petrov, VV; Slayman, CW | 3 |
| Board, PG; Gali, RR | 1 |
| Frillingos, S; Kaback, HR; Sun, J; Ujwal, ML | 1 |
| Bird, MI; Britten, CJ | 1 |
| Colman, RF; Hu, L | 1 |
| Giusti, L; Lucacchini, A; Mazzoni, MR; Rossi, E; Taddei, S | 1 |
| Capaldi, RA; Watts, SD | 1 |
| Capeyrou, R; Dietrich, G; Emorine, LJ; Gaibelet, G | 1 |
| Avidan, O; Boyer, PL; Gao, HQ; Hizi, A; Hughes, SH; Loya, S | 1 |
| Saxty, BA; van Heyningen, S | 1 |
| Babior, BM; Park, JW | 1 |
| Katiyar, SS; Pandey, A | 1 |
| Díaz, M; Riquelme, G; Sepúlveda, FV | 1 |
| Cotten, JF; Welsh, MJ | 2 |
| Botfield, MC; Kuroda, M; Tsuchiya, T; Weissborn, AC; Wilson, TH | 1 |
| Chung, CH; Kang, MS; Kim, HS; Kwon, JA; Seol, JH; Yoo, SJ | 1 |
| Ciucci, A; Manzini, S; Palma, C; Riitano, D; Werge, TM | 1 |
| Ferry, JG; Rasche, ME; Smith, KS | 1 |
| Eu, JP; Meissner, G; Stamler, JS; Xu, L | 1 |
| Pelham, HR; Scheel, AA | 1 |
| Chaconas, G; Millner, A | 1 |
| Kono, M; Oprian, DD; Yu, H | 1 |
| Kimura, T; Sawai, T; Shiina, Y; Yamaguchi, A | 1 |
| Chinenov, Y; Martin, ME; Schmidt, T; Yang, XY | 1 |
| Martin, J | 1 |
| Gaymard, E; Hoffman, BM; Johnson, MK; Knaff, DB; Schürmann, P; Staples, CR; Stritt-Etter, AL; Telser, J | 1 |
| Hamiche, A; Richard-Foy, H | 1 |
| Kawabe, T; Kimura, T; Nakatani, M; Yamaguchi, A | 1 |
| Herrero-Yraola, A; Jorcke, D; Schweiger, M; Ziegler, M | 1 |
| Blanot, D; Legrand, R; Masson, A; Nosal, F; Parquet, C; Schoot, B; van Heijenoort, J | 1 |
| Grunewald, M; Kanner, BI; Kavanaugh, MP; Zarbiv, R | 1 |
| Abboud, FM; Bates, JN; Bielefeldt, K; Chapleau, MW; Lee, HC; Li, Z | 1 |
| Burchell, A; Clottes, E | 1 |
| Matsumoto, M; Nakano, T; Terada, S; Yamaguchi, T | 1 |
| Chung, CH; Kim, HH; Lee, CS; Seol, JH; Seong, IS; Shimbara, N; Shin, DH; Tanaka, K; Yoo, SJ | 1 |
| Rao, DN; Reddy, YV | 1 |
| Amachi, T; Kino, K; Komano, T; Matsuo, M; Taguchi, Y; Takada, Y; Tucker, SJ; Ueda, K; Yamada, K | 1 |
| Abugo, OO; Balagopalakrishna, C; Horsky, J; Manoharan, PT; Nagababu, E; Rifkind, JM | 1 |
| Lynch, JW | 1 |
| Iwaki, S; Kimura-Someya, T; Yamaguchi, A | 1 |
| Hatsuzawa, K; Himeno, M; Nakayama, K; Tagaya, M; Tani, K; Yamaguchi, T | 1 |
| Kleineidam, RG; Kruse, S; Pommerencke, J; Roggentin, P; Schauer, R | 1 |
| Kanazawa, T; Suzuki, H | 1 |
| Swiedler, SJ; Wei, Z | 1 |
| Goto, S; Hashimoto, M; Majima, E; Shinohara, Y; Terada, H | 1 |
| Aledo, JC; Campos, JA; del Castillo-Olivares, A; del Valle, AE; Márquez, J; Núñez de Castro, I | 1 |
| Arthur, LO; Chertova, EN; Henderson, LE; Johnson, DG; Kane, BP; McGrath, C; Sowder, RC | 1 |
| Griffiths, G; Locker, JK | 1 |
| Gioannini, TL; Hiller, JM; Onoprishvili, I; Simon, EJ | 1 |
| Murayama, T; Naganuma, T; Nomura, Y | 1 |
| Hu, LA; King, SC | 1 |
| Hunke, S; Schneider, E | 1 |
| Darley-Usmar, VM; Hogg, N; Kalyanaraman, B; Matalon, S; Patel, RP; Spencer, NY | 1 |
| Chafin, DR; Hayes, JJ; Lee, KM | 1 |
| Granot, D; Lebendiker, M; Mordoch, SS; Schuldiner, S | 1 |
| Bouchard, BA; Furie, B; Furie, BC | 1 |
| Emi, Y; Ikushiro, S; Iyanagi, T; Kimura, S | 1 |
| Phelps, KK; Walker, RA | 1 |
| Huang, Y; Liu, M; Wang, E; Wang, Y; Wu, J | 1 |
| Jung, H; Quick, M; Stölting, S | 1 |
| Iwaki, S; Kimura-Someya, T; Konishi, S; Yamaguchi, A | 1 |
| Konings, WN; Lolkema, JS; Slotboom, DJ; Sobczak, I | 1 |
| Hamilton, SL; Porter Moore, C; Zhang, JZ | 1 |
| Martínez-Chantar, ML; Pajares, MA | 1 |
| Haniu, M; Rohde, MF; Rosenfeld, RD; Stark, KL; Young, Y; Zeni, L | 1 |
| Arana, ME; Boehmer, PE; Sampson, DA | 1 |
| Keller, K; Krause, G; Monden, I; Olsowski, A | 1 |
| Maekawa, M; Murayama, T; Nomura, Y; Satoh, S | 1 |
| Iwaki, S; Kimura-Someya, T; Konishi, S; Kubo, Y; Tamura, N; Yamaguchi, A | 1 |
| Polgár, L; Renner, V; Szeltner, Z | 1 |
| Barouki, R; Morel, Y | 1 |
| Murayama, T; Nomura, Y; Thang, SH; Umezawa, M; Yasuda, Y | 1 |
| Geertsma, ER; Knol, J; Poolman, B; Veenhoff, LM | 1 |
| Frillingos, S; Kaback, HR; Lawrence, MC; Sahin-Tóth, M | 1 |
| Harvey, JR; Klemm, MF; Lang, RJ; McPhee, GJ | 1 |
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| Bragg, PD; Hou, C | 1 |
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| Bray, CM; Waterworth, WM; West, CE | 1 |
| Hayakari, M; Kimura, J; Kumano, T; Sawamura, D; Tsuchida, S; Yamazaki, T | 1 |
| Hu, Y; Kaback, HR; Kwaw, I; Venkatesan, P | 1 |
| Hu, Y; Kaback, HR; Liu, Z; Venkatesan, P | 1 |
| Hu, Y; Kaback, HR; Venkatesan, P | 1 |
| Hara, N; Shimoyama, M; Terashima, M; Tsuchiya, M | 1 |
| Liu, L; Singh, TK | 1 |
| Dyllick-Brenzinger, M; Liu, M; Taylor, DE; Turner, RJ; Winstone, TL | 1 |
| Konev, SV; Mongin, AA; Nedvetsky, PI; Petrenko, SV; Rakovich, AA | 1 |
| Hunter, CN; Jensen, PE; Reid, JD | 1 |
| Ahvazi, B; Meighen, E; Szittner, R; Vrielink, A; Zhang, L | 1 |
| Berkner, KL; Crabb, JW; Hallgren, KW; Misono, KS; Miyagi, M; Pudota, BN; West, KA | 1 |
| Henderson, PJ; McDonald, TP | 1 |
| Choi, M; Hwang, IS; Kim, HJ; Park, SJ; Roh, T | 1 |
| Hamilton, SL; Reid, MB | 1 |
| Iwaki, S; Kimura-Someya, T; Konishi, S; Nada, S; Tamura, N; Yamaguchi, A | 1 |
| Arduini, RM; Baker, DP; Boriack-Sjodin, A; Carmillo, AN; Day, ES; Galdes, A; Garber, EA; Hronowski, X; Pepinsky, RB; Rayhorn, P; Shapiro, RI; Taylor, FR; Weinreb, PH; Wen, D; Whitty, A; Williams, KP | 1 |
| Boeren, S; de Wit, PJ; van den Burg, HA; van den Hooven, HW; Vervoort, J; Vossen, P | 1 |
| Cai, K; Itoh, Y; Khorana, HG | 1 |
| George, SP; Rao, MB | 1 |
| Di Stasi, MA; Mallozzi, C; Minetti, M | 1 |
| Helmkamp, GM; Li, H; Tremblay, JM; Yarbrough, LR | 1 |
| Kaback, HR; Sahin-Tóth, M; Weinglass, AB | 1 |
| Friedrich, CG; Quentmeier, A | 1 |
| Kitabatake, N; Wada, R | 1 |
| Blair, DF; Braun, TF | 1 |
| Goldblum, A; Goren, I; Honigman, A; Tavor, E | 1 |
| Lei, B; Tu, SC; Wei, CJ | 1 |
| Cappello, AR; Daddabbo, L; Miniero, DV; Natuzzi, D; Palmieri, F; Stipani, I; Stipani, V | 1 |
| Fujihira, E; Tamura, N; Yamaguchi, A | 1 |
| Acharya, SA; Bonaventura, C; Friedman, JM; Juszczak, LJ; Manjula, B | 1 |
| Galili, L; Kozachkov, L; Padan, E; Rimon, A; Rothman, A | 1 |
| Baggenstoss, BA; Kumari, K; Tlapak-Simmons, VL; Weigel, PH | 1 |
| Baggenstoss, BA; Heldermon, CD; Tlapak-Simmons, VL; Weigel, PH | 1 |
| Choi, EJ; Kang, SI; Kim, IY; Kim, JY; Park, HS | 1 |
| Ansorge, S; Arndt, M; Firla, B; Frank, K; Lendeckel, U; Täger, M; Thiel, U | 1 |
| Epps, DE; Vosters, AF | 1 |
| DeAngelis, PL; Pummill, PE | 1 |
| Bloom, D; Dhakshinamoorthy, S; Jaiswal, AK | 1 |
| Agbas, A; Chen, X; Hong, O; Kumar, KN; Michaelis, EK | 1 |
| English, AM; Murphy, ME; Tao, L | 1 |
| Guan, L; Kaback, HR; Sahin-Toth, M | 1 |
| Büyüknacar, HS; Dikmen, A; Göçmen, C; Onder, S; Resim, S | 1 |
| Giangregorio, N; Iacobazzi, V; Indiveri, C; Palmieri, F | 1 |
| Pitts, KE; Summers, AO | 1 |
| Dubocovich, MI; Gerdin, MJ; Mseeh, F | 1 |
| Dickson, KA; Hollenbach, AD; Washabaugh, MW | 1 |
| Konings, WN; Poelarends, GJ | 1 |
| Beckman, DL; Enright, E; Liu, L; Mehta, P; Sun, P; Terrian, DM; Tsai, SY | 1 |
| Driessen, AJ; Nouwen, N; van der Sluis, EO | 1 |
| Barnes, S; Falany, CN; Sakalian, M; Sfakianos, MK; Wilson, L | 1 |
| Apuzzo, S; Gros, P | 2 |
| Cotgreave, IA; Gerdes, R; Hamnell, Y; Holmgren, A; Lind, C; Schuppe-Koistinen, I; von Löwenhielm, HB | 1 |
| Emi, Y; Ikushiro, S; Iyanagi, T | 1 |
| Bal, N; Catty, P; Guillain, F; Mintz, E; Wu, CC | 1 |
| Czupryn, MJ; Marzilli, LA; Rouse, JC; Zhang, W | 1 |
| Angevine, CM; Fillingame, RH | 1 |
| Krom, BP; Lolkema, JS | 1 |
| Becker, D; Brand, P; Knop, J; Valk, E; Zahn, S | 1 |
| Kil, IS; Kim, SY; Lee, JH; Park, JW; Yang, ES | 1 |
| Bouhallab, S; Croguennec, T; Mehra, R; Mollé, D; O'Kennedy, BT | 1 |
| Guan, L; Hu, Y; Kaback, HR | 1 |
| Falke, JJ; Mehan, RS; White, NC | 1 |
| Hatanaka, T; Kubota-Akizawa, M; Mori, K; Negishi, T | 1 |
| Arroyo, A; Gandley, RE; Gryzunov, YA; Hubel, CA; Kagan, VE; Taylor, RN; Tyurin, VA; Vigne, JL; Vladimirov, YA; Zhao, Q | 1 |
| Althage, M; Karlsson, J; Rydström, J | 1 |
| Edmondson, DE; Hubalek, F; Pohl, J | 1 |
| Chang, XB; Cui, L; Hou, YX; Riordan, JR; Yang, R | 1 |
| Lolkema, JS; Sobczak, I | 1 |
| Jung, H; Landmeier, S; Pirch, T | 1 |
| Craik, DJ; Göransson, U | 1 |
| Dhar, SK; Jha, S; Karnani, N; Mukhopadhayay, G; Mukhopadhayay, K; Prasad, R; Saini, P; Shukla, S | 1 |
| CHANTRENNE, H | 1 |
| FLAVIN, M; SLAUGHTER, C | 1 |
| GRONINGER, HS | 1 |
| HOWARD-FLANDERS, P; JOHANSEN, I | 1 |
| STOREY, ID | 1 |
| Duurkens, RH; Geertsma, ER; Poolman, B | 1 |
| Jha, S; Karnani, N; Lynn, AM; Prasad, R | 1 |
| Tai, PC; Wu, KH | 1 |
| Hirata, T; Murakami, S; Saito, A; Tamura, N; Yamaguchi, A | 1 |
| Belyaeva, T; Faddejeva, M; Leontieva, E; Mozhenok, T; Shpakov, A | 1 |
| Angevine, CM; Fillingame, RH; Herold, KA | 1 |
| Heinze, M; Keller, K; Monden, I | 1 |
| Apuy, JL; Busenlehner, LS; Giedroc, DP; Russell, DH | 1 |
| Bouhallab, S; Croguennec, T; Mehra, R; Mollé, D | 1 |
| Cross, JV; Templeton, DJ | 1 |
| Kim, JE; Kim, JH; Kwon, J; Lee, CH; Lee, JW; Lee, SR; Park, EJ | 1 |
| Gamper, N; Li, Y; Shapiro, MS | 1 |
| García-Piñeres, AJ; Lindenmeyer, MT; Merfort, I | 1 |
| Boye, JI; Ismail, A; Ma, CY | 1 |
| Inoue, H; Kanazawa, H; Kuwabara, N; Nakamura, N; Tsuboi, Y | 1 |
| Maurer, MC; Sabo, TM; Turner, BT; Wilding, D | 1 |
| Av-Gay, Y; Fahey, RC; Newton, G; Rawat, M; Steffek, M; Uppal, M | 1 |
| Bowes, TJ; Gupta, RS | 1 |
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| Bauer, PJ; Krause, E | 1 |
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| Blache, D; Bourdon, E; Lagrost, L; Loreau, N | 1 |
| Cheah, J; Han, Y; Huang, Y; Huganir, RL; Juluri, K; Lowenstein, C; Luo, H; Man, HY; Sekine-Aizawa, Y; Snyder, SH | 1 |
| DeGrado, WF; Hemler, ME; Kovalenko, OV; Metcalf, DG | 1 |
| Boggs, JM; Culham, DE; Hillar, A; Vernikovska, YI; Wood, JM | 1 |
| Carbone, DL; Doorn, JA; Kiebler, Z; Petersen, DR | 1 |
| Benz, FW; Cai, J; Feng, W; Kang, YJ; Pierce, WM | 1 |
| Chen, C; Chen, Y; Huang, P; Li, JG; Liu-Chen, LY; Wang, Y | 1 |
| Ermolova, N; Kaback, HR; Madhvani, RV | 1 |
| Bachi, A; Camerini, S; Polci, ML | 1 |
| Pezzementi, L; Rowland, M; Tsigelny, I; Wolfe, M | 1 |
| Gleich, GJ; Nelson, CC; Ohnuki, LE; Parsawar, K; Wagner, LA | 1 |
| Dunten, R; Ermolova, N; Frillingos, S; Kaback, HR; Kwaw, I; Venkatesan, P; Zhang, W | 1 |
| Angevine, CM; Fillingame, RH; Herold, KA; Vincent, OD | 1 |
| Kanner, BI; Shlaifer, I | 1 |
| Flory, MR; Knee, KM; Mukerji, I; Roden, CK | 1 |
| Bjornsti, MA; Douat-Casassus, C; Montaudon, D; Palle, K; Pourquier, P; Quideau, S; Rivory, LP; Robert, J | 1 |
| Boggs, JM; Culham, DE; Keates, RA; Liu, F; Vernikovska, YI; Wood, JM | 1 |
| Das, S; Gavin, T; Geohagen, BC; LoPachin, RM | 1 |
| Ruf, W; Versteeg, HH | 1 |
| Guan, L; Kaback, HR | 1 |
| Ermolova, N; Kaback, HR; Nie, Y | 1 |
| Fillingame, RH; Steed, PR | 1 |
| Crankshaw, MW; Grant, GA | 1 |
| Forgac, M; Toei, M; Wang, Y | 1 |
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| Harayama, T; Shimizu, T; Shindou, H | 1 |
| Chowdhury, R; Flashman, E; Mecinović, J; Schofield, CJ | 1 |
| Haratake, M; Hongoh, M; Nakayama, M; Ono, M | 1 |
| Cao, K; Jiang, B; Tang, G; Wang, R; Wu, L | 1 |
| Becker, JM; Kim, H; Lee, BK; Naider, F | 1 |
| Wohlrab, H | 1 |
| Aram, L; Arbel, N; Geula, S; Shoshan-Barmatz, V | 1 |
| Kayastha, AM; Kumar, S | 1 |
| Hayakawa, T; Kawase, N; Kishi, J; Nakamura, H | 1 |
| Delaplane, S; Georgiadis, MM; Gross, ML; Kelley, MR; Luo, M; Rempel, DL; Su, D; Vu, B | 1 |
| Clarke, DJ; Hupp, TR; Langridge-Smith, PR; Mackay, CL; Sadler, PJ; Scotcher, J; Weidt, SK | 1 |
| Bauer, J; Fritsch, MJ; Palmer, T; Unden, G | 1 |
| Adams, A; Meiners, P; Rees, CA; Thompson, A; Woodbury, DJ | 1 |
| Forgac, M; Toei, M; Toei, S | 1 |
| Inamine, S; Matsushima, A; Nishimura, H; Shimohigashi, Y; Uemura, S | 1 |
| Lyon, RP; Meyer, DL; Senter, PD; Setter, JR | 1 |
| Niemann, J; Tsikas, D | 1 |
| Lu, L; Shimizu, T; Tanaka, K; Yokotani, K | 1 |
| Chen, C; Sun, Z; Tan, F; Tian, D; Tong, G; Yuan, S; Zhang, R; Zhuang, J | 1 |
| Berliner, JA; Cha, S; Gugiu, BG; Lee, S; Springstead, JR; Watson, AD | 1 |
| Anderson, CM; Choi, YE; Eskandari, S; Maestas, MJ; Omoto, JJ; Rahnama-Vaghef, A; Salto, G; Sanchez, RV | 1 |
| Forster, MJ; Sumien, N; Thangthaeng, N; Yan, LJ | 1 |
| Bönisch, H; Wenge, B | 1 |
| Samuels, HH; Tinnikov, AA | 1 |
| Beyer, SR; Boissel, JP; Closs, EI; Habermeier, A; Jaenecke, I; Mallmann, RT | 1 |
| Bechtold, E; Furdui, CM; King, SB; Poole, LB; Reisz, JA | 1 |
| Camp, DG; Gaffrey, MJ; Guo, J; Liu, T; Qian, WJ; Smith, RD; Su, D | 1 |
| Huh, Y; Jeong, NY; Jung, J; Kim, HW; Park, BS; Park, C; Rhyu, IJ; Yeo, SG | 1 |
| Chen, SR; Guo, W; Hiess, F; Jones, PP; Mi, T; Tang, Y; Wang, R; Wang, Y; Xiao, J; Xiao, Z; Zhang, JZ; Zhang, L | 1 |
| Tooker, RE; Vigh, J | 1 |
| Kiedrowski, L | 1 |
| Rogowska-Wrzesinska, A; Wojdyla, K | 1 |
| Chen, GJ; Dong, YB; Li, YA; Liu, JB; Liu, QK; Ma, JP; Zhang, S; Zhao, CW; Zhao, XD; Zhu, NX | 1 |
| Davies, MJ; Jongberg, S; Lund, MN; Pattison, DI; Skibsted, LH | 1 |
| Cai, R; Chen, C; Grewer, C; Guo, Z; Wang, J; Yuan, Y; Zhang, Z | 1 |
| Grant, GA | 1 |
| Fuller, W; Hemsley, PA; Hurst, CH; Plain, F; Turnbull, D | 1 |
| Ashcroft, AE; Beckstein, O; Calabrese, AN; Gsponer, J; Heinkel, F; Henderson, PJF; Jackson, SM; Jones, LN; Kokkinidou, M; Pearson, AR; Radford, SE; Sans, M; Sharples, D | 1 |
| Esparza, Y; Ullah, A; Wu, J | 1 |
| Beltrán González, AN; Calvo, DJ; Vicentini, F | 1 |
| Braakman, I; Hebert, DN; Lamriben, L; van Zadelhoff, G | 1 |
| Lametsch, R; Lu, H; Luo, Y | 1 |
| Ford, A; Ruan, X; Tavares-Carreón, F; Valvano, MA | 1 |
| McCoy, KM; McDermott, AE; Rogawski, R; Stovicek, O | 1 |
| Feng, Y; Liu, W; Qiu, F; Ru, J; Song, X; Wang, Y; Yang, Y; Zhang, G | 1 |
| Long, Y; Pan, Y; Shen, D; Yi, D; Zheng, H; Zheng, W | 1 |
| Chao, J; Huo, F; Xie, X; Yin, C; Yue, Y | 1 |
| Chen, W; Daniyal, M; Fan, J; Liu, B; Qin, Y; Shen, B; Sheng, WB; Tong, C; Wang, W; Xiao, F; Yang, W; Yang, Y; Yu, H; Zhou, J; Zhou, Q | 1 |
| Li, H; Li, Y; Pang, X; Wu, C; Xie, R; Yin, P; Zhou, Z | 1 |
| Hara, S; Ichinose, H; Inukai, S | 1 |
| Chen, PJ; DeVita, RJ; Dodatko, T; Houten, SM; Khamrui, S; Lazarus, MB; Leandro, J; Sanchez, R; Secor, C; Suebsuwong, C; Yu, C | 1 |
5 review(s) available for ethylmaleimide and cysteine
| Article | Year |
|---|---|
Topological studies of lactose permease of Escherichia coli by protein sequence analysis.
Topics: Affinity Labels; Amino Acid Sequence; Base Sequence; Binding Sites; Cell Membrane; Cysteine; Cytoplasm; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Immunologic Techniques; Iodine Radioisotopes; Membrane Transport Modulators; Membrane Transport Proteins; Molecular Weight; Monosaccharide Transport Proteins; Mutation; Peptide Fragments; Protein Conformation; Symporters; Thermolysin | 1985 |
Identification of specific amino acid residues.
Topics: Alkylating Agents; Amides; Amino Acid Sequence; Amino Acids; Arginine; Chromatography, Paper; Colorimetry; Cysteine; Cystine; Disulfides; Ethylmaleimide; Histidine; Hydroxymercuribenzoates; Indicators and Reagents; Mathematics; Mercury; Methionine; Methods; Oxidation-Reduction; Peptides; Proteins; Silver; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Tryptophan | 1970 |
RyR1 modulation by oxidation and calmodulin.
Topics: Alkylation; Animals; Binding Sites; Calcium Signaling; Calmodulin; Cysteine; Cystine; Ethylmaleimide; Hydrogen Peroxide; Ion Transport; Muscle Contraction; Muscle Proteins; Muscle, Skeletal; Myocardial Contraction; Myocardium; Nitric Oxide Donors; Oxidants; Oxidation-Reduction; Protein Binding; Protein Conformation; Protein Isoforms; Reactive Oxygen Species; Reducing Agents; Ryanodine Receptor Calcium Release Channel; Sarcoplasmic Reticulum; Sulfhydryl Compounds | 2000 |
The kamikaze approach to membrane transport.
Topics: Alkylation; Amino Acid Sequence; Bacterial Proteins; Binding Sites; Biological Transport; Carrier Proteins; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Humans; Lactose; Long QT Syndrome; Membrane Proteins; Membrane Transport Proteins; Metabolism, Inborn Errors; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protons; Recombinant Fusion Proteins; Structure-Activity Relationship; Sulfhydryl Reagents; Symporters | 2001 |
Differential alkylation-based redox proteomics--Lessons learnt.
Topics: Alkylation; Animals; Arsenites; Ascorbic Acid; Cysteine; Dithiothreitol; Ethylmaleimide; Eukaryotic Cells; Humans; Iodoacetamide; Models, Biological; Oxidation-Reduction; Phosphines; Proteomics; Staining and Labeling; Sulfhydryl Compounds | 2015 |
649 other study(ies) available for ethylmaleimide and cysteine
| Article | Year |
|---|---|
Purification, stability and inhibition of the collagenase from Achromobacter iophagus.
Topics: Alcaligenes; Caseins; Cysteine; Edetic Acid; Ethylmaleimide; Histidine; Hydrogen-Ion Concentration; Microbial Collagenase; Temperature | 1975 |
The interaction of aurothiomalate and cysteine.
Topics: Chemical Phenomena; Chemistry; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Gold Radioisotopes; Gold Sodium Thiomalate; Humans; Hydrogen-Ion Concentration; Serum Albumin; Sulfhydryl Reagents; Time Factors | 1976 |
Activation of hepatic guanylate cyclase by N-methyl-N'-nitro-N-nitrosoguanidine. Effects of thiols, N-ethylmaleimide, and divalent cations.
Topics: Anaerobiosis; Animals; Ascorbic Acid; Cysteine; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Guanylate Cyclase; In Vitro Techniques; Kinetics; Liver; Magnesium; Male; Manganese; Methylnitronitrosoguanidine; Rats; Sulfhydryl Compounds | 1977 |
Reaction of yeast fatty acid synthetase with iodoacetamide. 1. Kinetics of inactivation and extent of carboxamidomethylation.
Topics: Cysteine; Ethylmaleimide; Fatty Acid Synthases; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Kinetics; Saccharomyces cerevisiae; Sulfhydryl Reagents | 1977 |
Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli W.
Topics: Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Ethylmaleimide; Kinetics; Magnesium; Malate Dehydrogenase; NADP; Spectrophotometry, Ultraviolet; Sulfhydryl Reagents; Thermodynamics | 1979 |
Further studies on the possibility of differential demonstration of radicals of sulphur-containing amino-acids.
Topics: Amino Acids; Animals; Brain; Brain Chemistry; Cysteine; Ethylmaleimide; Homocystine; Liver; Methionine; Rats; Staining and Labeling | 1976 |
The sulfhydryl groups involved in the active site of myosin B adenosinetriphosphatase. V. The possible localization of Sa and other sulfhydryl groups in myosin rods.
Topics: Adenosine Triphosphatases; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Kinetics; Molecular Weight; Muscles; Myosins; Protein Binding; Rabbits; Sulfhydryl Compounds | 1979 |
Non-cooperative response of actin-cystein 373 in cooperatively behaving regulated actin filaments.
Topics: Actins; Adenosine Triphosphatases; Animals; Cysteine; Dithiothreitol; Ethylmaleimide; Fluorescent Dyes; Iodoacetamide; Kinetics; Magnesium; Naphthalenesulfonates; Protein Binding; Spectrometry, Fluorescence | 1979 |
Comparative structural studies of the active site of ATP: guanidine phosphotransferases. The essential cysteine tryptic peptide of taurocyamine kinase from Arenicola marina.
Topics: Alkanesulfonates; Amino Acid Sequence; Amino Acids; Animals; Binding Sites; Cysteine; Ethylmaleimide; Guanidines; Peptide Fragments; Phosphotransferases; Polychaeta; Protein Binding; Subtilisins; Succinimides; Taurocholic Acid; Thermolysin; Trypsin | 1975 |
Inability of organomercurials to inhibit the reaction between aurothiomalate and thiols.
Topics: 4-Chloromercuribenzenesulfonate; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Gold Sodium Thiomalate; Phenylmercury Compounds; Serum Albumin | 1976 |
Conformational motility in D-glyceraldehyde-3-phosphate dehydrogenase influenced by subunit interactions.
Topics: Animals; Binding Sites; Cysteine; Ethylmaleimide; Glyceraldehyde-3-Phosphate Dehydrogenases; Kinetics; Macromolecular Substances; Mercuribenzoates; Muscles; NAD; Protein Binding; Protein Conformation; Swine | 1976 |
Effect of modification of SH-groups in D-glyceraldehyde-3-phosphate dehydrogenase on the properties of enzyme--coenzyme complex.
Topics: Animals; Binding Sites; Cysteine; Ethylmaleimide; Glyceraldehyde-3-Phosphate Dehydrogenases; Iodoacetates; Kinetics; Mercuribenzoates; Muscles; NAD; Protein Binding; Protein Conformation; Sulfhydryl Reagents; Swine | 1976 |
Mannosidostreptomycin hydrolase.
Topics: Arsenates; Azides; Cations, Divalent; Cyanides; Cysteine; Disaccharidases; Enzyme Activation; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Mannosidases; Mannosides; Methods; Streptomyces; Streptomycin; Subcellular Fractions; Sulfites | 1975 |
Chemical modification of bovine heart mitochondrial malate dehydrogenase. Selective modification of cysteine and histidine.
Topics: Amino Acids; Animals; Carbon Radioisotopes; Cattle; Cysteine; Ethylmaleimide; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Malate Dehydrogenase; Malates; Mitochondria, Muscle; Molecular Weight; Myocardium; NAD; Oxaloacetates; Urea | 1975 |
Glutamine aspartate transaminase modified at cysteine 390 with enriched carbon-13 cyanide.
Topics: Animals; Aspartate Aminotransferases; Carbon Isotopes; Chemical Phenomena; Chemistry; Cyanides; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Myocardium; Swine | 1975 |
The action of N-ethylmaleimide on the membrane currents and contractile tension in the bullfrog atrium.
Topics: Action Potentials; Animals; Anura; Calcium; Cysteine; Ethylmaleimide; Heart; Heart Atria; In Vitro Techniques; Myocardial Contraction; Rana catesbeiana; Stimulation, Chemical | 1978 |
The reactivity of one essential cysteine as a conformational probe in Escherichia coli tryptophanase. Application to the study of the structural influence of subunit interactions.
Topics: Apoproteins; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Kinetics; Lyases; Macromolecular Substances; Peptide Fragments; Potassium; Protein Binding; Sodium; Spectrometry, Fluorescence; Trypsin; Tryptophanase | 1977 |
Chorismate mutase/prephenate dehydratase from Escherichia coli K12. Effects of chemical modification on the enzymic activities and allosteric inhibition.
Topics: Allosteric Regulation; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Hydro-Lyases; Kinetics; Phenylalanine; Prephenate Dehydratase; Sulfhydryl Reagents | 1977 |
Activity changes in lac repressor with cysteine oxidation.
Topics: Amino Acids; Bromosuccinimide; Cysteine; DNA, Bacterial; Escherichia coli; Ethylmaleimide; Kinetics; Oxidation-Reduction; Protein Binding; Repressor Proteins; Transcription Factors | 1979 |
Chemical modification of lactose repressor protein using N-substituted maleimides.
Topics: Cysteine; Escherichia coli; Ethylmaleimide; Maleimides; Protein Binding; Protein Conformation; Repressor Proteins; Structure-Activity Relationship; Transcription Factors | 1979 |
Studies on the reactivity of the essential cysteine of thymidylate synthetase.
Topics: Binding Sites; Cysteine; Ethylmaleimide; Iodoacetates; Kinetics; Lacticaseibacillus casei; Methyltransferases; Sulfhydryl Reagents; Thymidylate Synthase | 1977 |
The effect of sulfhydryl group reagents on the permeation of mitochondrial aspartate aminotransferase into mitochondria in vitro.
Topics: Animals; Aspartate Aminotransferases; Biological Transport; Cysteine; Ethylmaleimide; Kinetics; Mersalyl; Mitochondria, Liver; Permeability; Rats; Sulfhydryl Reagents | 1979 |
Determination of hydrogen sulfide with 5,5'-dithiobis-(2-nitrobenzoic acid), N-ethylmaleimide, and parachloromercuribenzoate.
Topics: Chloromercuribenzoates; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydrogen Sulfide; Methods; Muramidase; Nitrobenzoates; Proteins; Sulfhydryl Compounds; Sulfur | 1977 |
Loss of Cu2+-binding to actin upon removal of the C-terminal phenylalanine by carboxypeptidase A.
Topics: Actins; Carboxypeptidases; Copper; Cysteine; Ethylmaleimide; Phenylalanine; Protein Binding | 1977 |
Usefulness of N-ethylmaleimide in the identification of 75Se-labeled selenocysteine.
Topics: Chromatography, Paper; Cysteine; Ethylmaleimide; Methods; Proteins; Radioisotopes; Selenium | 1977 |
Further studies on irreversible inhibition of dopamine-beta-hydroxylase by cysteine.
Topics: Catalase; Copper; Cysteine; Dopamine beta-Hydroxylase; Ethylmaleimide; Glutathione; In Vitro Techniques; Kinetics; Temperature; Tyramine | 1976 |
Irreversible inhibition of dopamine-beta-hydroxylase by cysteine.
Topics: Adrenal Glands; Animals; Catalase; Cattle; Chelating Agents; Cysteine; Dialysis; Dopamine beta-Hydroxylase; Ethylmaleimide; In Vitro Techniques; Peroxides; Solutions | 1976 |
Different patterns of inhibition of dopamine-beta-hydroxylase by cysteine.
Topics: Adrenal Medulla; Animals; Cattle; Copper; Cysteine; Dopamine beta-Hydroxylase; Ethylmaleimide; In Vitro Techniques; Tyramine | 1976 |
A simple procedure for measuring small amounts of (14C)N-ethylmaleimide.
Topics: Binding Sites; Carbon Radioisotopes; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Kinetics; Methods; Microchemistry | 1976 |
The reaction of cytochalasin A with sulfhydryl groups.
Topics: Binding Sites; Cysteine; Cytochalasins; Ethylmaleimide; Glutathione; Kinetics; L-Lactate Dehydrogenase; Ovalbumin; Protein Binding; Protein Denaturation; Sodium Dodecyl Sulfate; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 1976 |
N-ethylmaleimide-induced conductance changes in the giant neuron of Aplysia.
Topics: Action Potentials; Animals; Cell Membrane; Cell Membrane Permeability; Chlorides; Cysteine; Dithiothreitol; Ethylmaleimide; Ganglia; In Vitro Techniques; Membrane Potentials; Mollusca; Neurons; Potassium; Sodium | 1975 |
Reactivity of the cysteine and tyrosine residues of aspartate transaminase from chicken heart cytosol.
Topics: Animals; Aspartate Aminotransferases; Binding Sites; Chickens; Cysteine; Cytosol; Disulfides; Dithionitrobenzoic Acid; Ethylmaleimide; Iodoacetamide; Kinetics; Mercuribenzoates; Myocardium; Protein Binding; Pyridines; Sulfhydryl Compounds; Tetranitromethane; Tyrosine | 1975 |
Blockade by metal complexing agents and by catalase of the effects of arachidonic acid on platelets: relevance to the study of anti-inflammatory mechanisms.
Topics: Adenosine Diphosphate; Amitrole; Animals; Anti-Inflammatory Agents; Aorta; Arachidonic Acids; Azides; Blood Platelets; Catalase; Chelating Agents; Chloromercuribenzoates; Copper; Cysteine; Ethylmaleimide; In Vitro Techniques; Muscle Contraction; Platelet Aggregation; Rabbits; Rats; Stomach; Zinc | 1975 |
[Biosynthesis of glutathione. VI. Ocurrence and level of gamma-glutamylcysteine in human red cells (author's transl)].
Topics: Cysteine; Erythrocytes; Ethylmaleimide; Glutathione; Humans; Peptide Synthases | 1975 |
Chemical modification as a probe of the topography and reactivity of horse-spleen apoferritin.
Topics: Amides; Anhydrides; Animals; Apoferritins; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Ferritins; Glycine; Guanidines; Horses; Iodoacetates; Kinetics; Lysine; Maleates; Protein Binding; Protein Conformation; Spleen | 1976 |
Automated microanalysis of sulfur containing amino acids and their derivatives.
Topics: Amino Acids; Autoanalysis; Cells, Cultured; Cysteine; Cystinosis; Disulfides; Ethylmaleimide; Fibroblasts; Glutathione; Homocysteine; Humans; Methionine; Methods; Sulfur | 1976 |
Bovine inositol monophosphatase. Modification, identification and mutagenesis of reactive cysteine residues.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cattle; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Fluorescence Polarization; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis; Peptide Mapping; Phosphoric Monoester Hydrolases | 1992 |
Conserved cysteine residue in the DNA-binding domain of the bovine papillomavirus type 1 E2 protein confers redox regulation of the DNA-binding activity in vitro.
Topics: Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Bovine papillomavirus 1; Cysteine; Diamide; Disulfides; DNA-Binding Proteins; Ethylmaleimide; Molecular Sequence Data; Oligonucleotide Probes; Oncogene Proteins, Viral; Open Reading Frames; Plasmids; Protein Biosynthesis; Rabbits; Reticulocytes; Sequence Homology, Nucleic Acid; Sulfhydryl Reagents; Transcription, Genetic | 1992 |
Bovine inositol monophosphatase; observation of the modification of a cysteine residue using protein fluorescence.
Topics: Animals; Brain; Cattle; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Humans; Macromolecular Substances; Molecular Weight; Mutagenesis, Site-Directed; Phosphoric Monoester Hydrolases; Rats; Spectrometry, Fluorescence | 1992 |
Correlation of haemagglutination activity with trypsin-like protease activity of Porphyromonas gingivalis.
Topics: Animals; Cysteine; Dithiothreitol; Ethylmaleimide; Fusobacterium nucleatum; Gingiva; Hemagglutination; Humans; Macaca fascicularis; Mutagens; Nitrosoguanidines; Phenotype; Phenylmethylsulfonyl Fluoride; Porphyromonas gingivalis; Tosyllysine Chloromethyl Ketone; Trypsin | 1992 |
Role of the reactive cysteine residue in restriction endonuclease Cfr9I.
Topics: Base Sequence; Cysteine; Deoxyribonucleases, Type II Site-Specific; Dithionitrobenzoic Acid; DNA Restriction Enzymes; Ethylmaleimide; Hydrogen-Ion Concentration; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Oligonucleotides | 1992 |
F1-ATPase with cysteine instead of serine at residue 373 of the alpha subunit.
Topics: Amino Acid Sequence; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Genotype; Kinetics; Macromolecular Substances; Proton-Translocating ATPases; Serine; Species Specificity | 1992 |
A novel mechanism for regulation of vacuolar acidification.
Topics: Animals; Brain; Cattle; Clathrin; Coated Pits, Cell-Membrane; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Hydrogen-Ion Concentration; Oxidation-Reduction; Proton-Translocating ATPases; Vacuoles | 1992 |
Antibody and peptide probes of interactions between the SH1-SH2 region of myosin subfragment 1 and actin's N-terminus.
Topics: Actins; Adenosine Triphosphatases; Amino Acid Sequence; Animals; Antibodies; Binding Sites; Calcium-Transporting ATPases; Cation Transport Proteins; Cysteine; Electrochemistry; Ethylmaleimide; Kinetics; Molecular Sequence Data; Myosin Subfragments; Oligopeptides; Peptide Fragments; Polymers; Rabbits | 1992 |
Characterization of high affinity binding between laminin and Alzheimer's disease amyloid precursor proteins.
Topics: Alzheimer Disease; Amyloid beta-Protein Precursor; Binding, Competitive; Cations, Divalent; Chlorides; Cysteine; Enzyme-Linked Immunosorbent Assay; Ethylmaleimide; Heparan Sulfate Proteoglycans; Heparitin Sulfate; Humans; Laminin; Proteoglycans; Zinc; Zinc Compounds; Zinc Fingers | 1992 |
Identification and localization of a cysteinyl residue critical for the trypsin-like catalytic activity of the proteasome.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cattle; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Chymotrypsin; Cyanogen Bromide; Cysteine; Cysteine Endopeptidases; Erythrocytes; Ethylmaleimide; Humans; Kinetics; Leupeptins; Macromolecular Substances; Molecular Sequence Data; Multienzyme Complexes; Myocardium; Peptide Fragments; Peptide Mapping; Proteasome Endopeptidase Complex; Trypsin | 1992 |
Introduction of reactive cysteine residues in the epsilon subunit of Escherichia coli F1 ATPase, modification of these sites with tetrafluorophenyl azide-maleimides, and examination of changes in the binding of the epsilon subunit when different nucleotid
Topics: Adenosine Triphosphate; Azides; Base Sequence; Binding Sites; Cross-Linking Reagents; Cysteine; Edetic Acid; Escherichia coli; Ethylmaleimide; Fluorobenzenes; Magnesium; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Photolysis; Proton-Translocating ATPases | 1992 |
Cysteine 254 of the 73-kDa A subunit is responsible for inhibition of the coated vesicle (H+)-ATPase upon modification by sulfhydryl reagents.
Topics: Amino Acid Sequence; Animals; Binding Sites; Brain; Cattle; Clathrin; Coated Pits, Cell-Membrane; Cysteine; Dithiothreitol; Ethylmaleimide; Kinetics; Macromolecular Substances; Molecular Sequence Data; Molecular Weight; Proton-Translocating ATPases; Sulfhydryl Reagents | 1992 |
Purification and properties of an ATPase from Sulfolobus solfataricus.
Topics: 4-Chloro-7-nitrobenzofurazan; Adenosine Triphosphatases; Anions; Chloromercuribenzoates; Cysteine; Enzyme Activation; Ethylmaleimide; Intracellular Membranes; Protein Conformation; Sulfites; Sulfolobus; Vacuoles | 1992 |
Inactivation of pig heart NADP-specific isocitrate dehydrogenase by two affinity reagents is due to reaction with a cysteine not essential for function.
Topics: Adenosine Diphosphate; Affinity Labels; Amino Acid Sequence; Animals; Cysteine; Ethylmaleimide; Isocitrate Dehydrogenase; Ketoglutaric Acids; Mitochondria, Heart; Molecular Sequence Data; Swine | 1992 |
Role of intracellular thiols in release of EDRF from cultured endothelial cells.
Topics: Adenosine Diphosphate; Animals; Aorta; Arginine; Biological Assay; Cattle; Cells, Cultured; Cysteine; Diamide; Endothelium, Vascular; Ethylmaleimide; Glutathione; Ionomycin; Kinetics; Nitric Oxide; Nitroarginine; omega-N-Methylarginine; Sulfhydryl Compounds; Sulfhydryl Reagents | 1992 |
Effect of group-selective modification reagents on arylamine N-acetyltransferase activities.
Topics: Animals; Arginine; Arylamine N-Acetyltransferase; Binding Sites; Cricetinae; Cysteine; Diethyl Pyrocarbonate; Enzyme Activation; Ethylmaleimide; Histidine; Hydroxylamine; Hydroxylamines; Liver; Male; Mesocricetus; Phenylglyoxal | 1992 |
Active site thiol(s) in Leishmania donovani adenosine kinase: comparison with hamster enzyme and evidence for the absence of regulatory adenosine binding site.
Topics: Adenosine; Adenosine Kinase; Animals; Binding Sites; Cricetinae; Cysteine; Ethylmaleimide; Kinetics; Leishmania donovani; Sulfhydryl Compounds | 1992 |
Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine, and glutathione in human plasma.
Topics: Borohydrides; Chromatography, Liquid; Cysteine; Dipeptides; Ethylmaleimide; Female; Glutathione; Homocysteine; Humans; Indicators and Reagents; Male; Oxidation-Reduction; Reference Values; Sex Characteristics; Spectrometry, Fluorescence | 1992 |
Roles of cysteinyl residues of phosphoribulokinase as examined by site-directed mutagenesis.
Topics: Base Sequence; Blotting, Western; Cysteine; DNA; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Plants | 1991 |
Evidence for essential histidine and cysteine residues in calcium/calmodulin-sensitive cyclic nucleotide phosphodiesterase.
Topics: Animals; Brain; Catalysis; Cattle; Cyclic Nucleotide Phosphodiesterases, Type 1; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Histidine; Hydrogen-Ion Concentration; Kinetics; Phosphoric Diester Hydrolases; Spectrophotometry, Ultraviolet | 1991 |
Characterization of SH groups in porin of bovine heart mitochondria. Porin cysteines are localized in the channel walls.
Topics: Animals; Bacterial Outer Membrane Proteins; Cattle; Cysteine; Ethylmaleimide; Indicators and Reagents; Intracellular Membranes; Ion Channels; Mitochondria, Heart; Molecular Weight; Porins; Protein Binding; Submitochondrial Particles; Sulfhydryl Compounds | 1991 |
Reaction product inactivation of aryl sulfotransferase IV following electrophilic substitution by the sulfuric acid ester of N-hydroxy-2-acetylaminofluorene.
Topics: Animals; Arylsulfotransferase; Cysteine; Esters; Ethylmaleimide; Hydroxyacetylaminofluorene; Liver; Methionine; Rats | 1992 |
Affinity-chromatographic purification of sixteen cysteine-substituted maltoporin variants: thiol reactivity and cross-linking in an outer membrane protein of Escherichia coli.
Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Chromatography, Affinity; Cysteine; Escherichia coli; Ethylmaleimide; Molecular Sequence Data; Mutagenesis; Oxidation-Reduction; Porins; Receptors, Virus; Sulfhydryl Compounds; Temperature | 1991 |
Cysteinyl peptides of pig heart NADP-dependent isocitrate dehydrogenase that are modified upon inactivation by N-ethylmaleimide.
Topics: Amino Acid Sequence; Animals; Chromatography, High Pressure Liquid; Cysteine; Ethylmaleimide; Isocitrate Dehydrogenase; Molecular Sequence Data; Myocardium; NADP; Peptide Mapping; Swine; Trypsin | 1991 |
Reversible inactivation of recombinant rat liver guanidinoacetate methyltransferase by glutathione disulfide.
Topics: Adenosine; Animals; Cysteine; Enzyme Activation; Enzyme Reactivators; Ethylmaleimide; Glutathione; Glutathione Disulfide; Glycine; Guanidinoacetate N-Methyltransferase; Iodoacetates; Iodoacetic Acid; Kinetics; Liver; Mercaptoethanol; Methyltransferases; Oxidation-Reduction; Rats; Recombinant Proteins; S-Adenosylmethionine; Spectrum Analysis; Sulfhydryl Compounds | 1991 |
Role of cysteine residues in the activity of rat glutathione transferase P (7-7): elucidation by oligonucleotide site-directed mutagenesis.
Topics: Animals; Base Sequence; Cystamine; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethylmaleimide; Gene Expression; Glutathione Transferase; Kinetics; Liver; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligonucleotides; Plasmids; Rats; Recombinant Proteins; Structure-Activity Relationship | 1991 |
Inhibition of ADP-induced platelet shape change and aggregation by o-phthalaldehyde: evidence for covalent modification of cysteine and lysine residues.
Topics: Adenosine; Adenosine Diphosphate; Affinity Labels; Blood Platelets; Cysteine; Ethylmaleimide; Humans; Kinetics; Lysine; o-Phthalaldehyde; Platelet Aggregation; Platelet Aggregation Inhibitors; Spectrometry, Fluorescence | 1991 |
Probing the role of cysteine residues in the CheR methyltransferase.
Topics: Base Sequence; Cysteine; Dithionitrobenzoic Acid; DNA; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Kinetics; Methyltransferases; Molecular Sequence Data; Mutagenesis, Site-Directed; Sulfhydryl Compounds; Temperature | 1991 |
Sulfhydryl-dependent attachment of Treponema denticola to laminin and other proteins.
Topics: Amino Acid Sequence; Animals; Bacterial Adhesion; Binding Sites; Cysteine; Epithelium; Ethylmaleimide; Extracellular Matrix Proteins; Glutathione; Hot Temperature; Hydrogen-Ion Concentration; Laminin; Microscopy, Electron, Scanning; Molecular Sequence Data; Oligopeptides; Osmolar Concentration; Periodontal Ligament; Protein Binding; Sulfhydryl Compounds; Swine; Treponema | 1991 |
Mitochondrial phosphate transport. The Saccharomyces cerevisiae (threonine 43 to cysteine) mutant protein explicitly identifies transport with genomic sequence.
Topics: Base Sequence; Carrier Proteins; Catalysis; Cysteine; DNA; Ethylmaleimide; Hydrogen-Ion Concentration; Mitochondria; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Phosphate-Binding Proteins; Plasmids; Polymerase Chain Reaction; Saccharomyces cerevisiae; Threonine | 1991 |
Effect of hydration in metHb: reversible changes monitored by ESR of iron.
Topics: Animals; Cattle; Cysteine; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Ferric Compounds; Ferrous Compounds; Freeze Drying; Hemeproteins; Hemoglobins; Humans; Iron; Methemoglobin; Protein Conformation; Water | 1990 |
The role of cysteine-150 in the structure and activity of rat liver S-adenosyl-L-methionine synthetase.
Topics: Amino Acids; Animals; Chromatography, High Pressure Liquid; Cysteine; Ethylmaleimide; Kinetics; Liver; Methionine Adenosyltransferase; Molecular Weight; Peptide Fragments; Peptide Mapping; Protein Binding; Rats; Trypsin; Urea | 1991 |
The functional role of cysteines in isopenicillin N synthase. Correlation of cysteine reactivities toward sulfhydryl reagents with kinetic properties of cysteine mutants.
Topics: Acremonium; Amino Acid Sequence; Catalysis; Chromatography, High Pressure Liquid; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Kinetics; Molecular Sequence Data; Mutation; Oxidation-Reduction; Oxidoreductases; Protein Conformation; Sulfhydryl Compounds; Trypsin | 1991 |
Properties of a novel nitric oxide-stimulated ADP-ribosyltransferase.
Topics: Adenosine Diphosphate Ribose; Blood Platelets; Cysteine; Cytosol; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Humans; Nitric Oxide; Nitroprusside; Poly(ADP-ribose) Polymerases | 1990 |
MgADP-induced changes in the structure of myosin S1 near the ATPase-related thiol SH1 probed by cross-linking.
Topics: Adenosine Diphosphate; Calcium-Transporting ATPases; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Cysteine; Ethylmaleimide; Kinetics; Maleimides; Myosin Subfragments; Myosins; Peptide Fragments | 1990 |
Multiple modes of activation of latent human fibroblast collagenase: evidence for the role of a Cys73 active-site zinc complex in latency and a "cysteine switch" mechanism for activation.
Topics: Amino Acid Sequence; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Fibroblasts; Gingiva; Humans; Kinetics; Microbial Collagenase; Models, Structural; Molecular Sequence Data; Protein Binding; Protein Conformation; Zinc | 1990 |
Identification of different classes of nonessential sulfhydryl groups in Escherichia coli adenylosuccinate synthetase.
Topics: Adenylosuccinate Synthase; Binding Sites; Chromatography, High Pressure Liquid; Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Electron Spin Resonance Spectroscopy; Escherichia coli; Ethylmaleimide; Kinetics; Ligases; Nitrobenzoates; Peptide Fragments; Sulfhydryl Compounds; Trypsin | 1990 |
Effects of sulfhydryl-modifying reagents, 3-nitro-2-pyridinesulfenyl compounds, on the coupling between inhibitory receptors and GTP-binding proteins Gi/Go in rat brain membranes.
Topics: 8-Hydroxy-2-(di-n-propylamino)tetralin; Animals; Brain; Cell Membrane; Clonidine; Cysteine; Ethylmaleimide; GTP-Binding Proteins; Guanosine Triphosphate; Indicators and Reagents; Male; Nitro Compounds; Pertussis Toxin; Pyridines; Rats; Receptors, Adrenergic, alpha; Receptors, Serotonin; Sulfhydryl Compounds; Tetrahydronaphthalenes; Virulence Factors, Bordetella | 1990 |
Identification of a critical cysteine in EcoRI DNA methyltransferase by mass spectrometry.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Deoxyribonuclease EcoRI; Dipeptides; DNA Restriction Enzymes; Escherichia coli; Ethylmaleimide; Kinetics; Mass Spectrometry; Molecular Sequence Data; Peptide Fragments; Staphylococcus aureus | 1990 |
Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification.
Topics: Amino Acid Sequence; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Endoribonucleases; Escherichia coli; Ethylmaleimide; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Mapping; Ribonuclease H; Serine Endopeptidases; Structure-Activity Relationship | 1990 |
Methylmercury uptake in rat primary astrocyte cultures: the role of the neutral amino acid transport system.
Topics: Amino Acids; Animals; Animals, Newborn; Astrocytes; Cells, Cultured; Cerebral Cortex; Cysteine; Ethylmaleimide; Methylmercury Compounds; Rats | 1990 |
Free thiols of platelet thrombospondin. Evidence for disulfide isomerization.
Topics: Amino Acid Sequence; Blood Platelets; Cysteine; Disulfides; Ethylmaleimide; Humans; Iodoacetamide; Isomerism; Kinetics; Molecular Sequence Data; Platelet Membrane Glycoproteins; Sequence Homology, Nucleic Acid; Thrombospondins | 1990 |
Mitochondrial phosphate transport. N-ethylmaleimide insensitivity correlates with absence of beef heart-like Cys42 from the Saccharomyces cerevisiae phosphate transport protein.
Topics: Amino Acid Sequence; Animals; Biological Transport; Candida; Carrier Proteins; Cattle; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Liposomes; Mersalyl; Mitochondria; Mitochondria, Heart; Molecular Sequence Data; Peptide Mapping; Phosphate-Binding Proteins; Phosphates; Saccharomyces cerevisiae | 1990 |
Cellular recovery of glyceraldehyde-3-phosphate dehydrogenase activity and thiol status after exposure to hydroperoxides.
Topics: Cell Line; Cysteamine; Cysteine; Cystine; Dithiothreitol; Ethacrynic Acid; Ethylmaleimide; Glutathione; Glutathione Disulfide; Glyceraldehyde-3-Phosphate Dehydrogenases; Humans; Hydrogen Peroxide; Kinetics; Lung Neoplasms; Oxidation-Reduction; Peroxides; Sulfhydryl Compounds; tert-Butylhydroperoxide; Ultrasonics | 1990 |
Selective molybdate-directed covalent modification of sulfhydryl groups in the steroid-binding versus the DNA-binding domain of the glucocorticoid receptor.
Topics: Affinity Labels; Animals; Binding Sites; Cysteine; Cytosol; Dexamethasone; DNA-Binding Proteins; Ethylmaleimide; Hydrogen Peroxide; Kinetics; L Cells; Methyl Methanesulfonate; Mice; Molecular Weight; Molybdenum; Receptors, Glucocorticoid; Sulfhydryl Compounds; Sulfhydryl Reagents | 1990 |
Tumor-promoting phorbol esters and cell proliferation stimulate secretion of basement membrane (type IV) collagen-degrading metalloproteinase by human fibroblasts.
Topics: Aprotinin; Basement Membrane; Cell Division; Culture Media; Cysteine; Edetic Acid; Endopeptidases; Ethylmaleimide; Fibroblasts; Humans; Metalloendopeptidases; Microbial Collagenase; Phorbols; Procollagen; Skin; Tetradecanoylphorbol Acetate; Time Factors; Trypsin | 1985 |
The influence of the sesquiterpene lactones from Geigeria on mast cell degranulation.
Topics: Alkylation; Animals; Cysteine; Ethylmaleimide; Histamine Release; Lactones; Male; Mast Cells; p-Methoxy-N-methylphenethylamine; Plant Extracts; Rats; Rats, Inbred Strains; Sesquiterpenes; Sesquiterpenes, Guaiane; Thapsigargin | 1987 |
Nucleosomal structure as probed by H3 histone thiol reactivity. Conformation of H3 histone variants is differently affected by thiol group reagents.
Topics: Acetylation; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; HeLa Cells; Histones; Humans; Nucleosomes; Protein Conformation | 1987 |
Functional cysteinyl residues in human placental aldose reductase.
Topics: Aldehyde Reductase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; Female; Humans; Kinetics; Models, Theoretical; Placenta; Pregnancy; Protein Binding; Substrate Specificity; Sugar Alcohol Dehydrogenases | 1989 |
Conformational modification of muscle phosphofructokinase from Jaculus orientalis upon ligand binding.
Topics: Adenosine Monophosphate; Adenosine Triphosphate; Allosteric Regulation; Animals; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Fructosephosphates; Inosine Triphosphate; Muscles; Phosphofructokinase-1; Protein Conformation; Rodentia; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 1989 |
Cysteine 532 and cysteine 545 are the N-ethylmaleimide-reactive residues of the Neurospora plasma membrane H+-ATPase.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cysteine; Cytoplasm; Ethylmaleimide; Fungal Proteins; Methyl Methanesulfonate; Molecular Sequence Data; Naphthalenesulfonates; Neurospora; Neurospora crassa; Peptide Fragments; Proton-Translocating ATPases; Rabbits; Rats; Sequence Homology, Nucleic Acid; Sheep; Swine | 1989 |
Role of cysteine 41 of the A subunit of pertussis toxin.
Topics: Animals; Cell Line; Cell Membrane; Cysteine; Disulfides; Ethylmaleimide; Macromolecular Substances; Membrane Proteins; Molecular Weight; NAD+ Nucleosidase; Pertussis Toxin; Protein Conformation; Virulence Factors, Bordetella | 1989 |
The redox state of cysteines 201 and 317 of the erythrocyte anion exchanger is critical for ankyrin binding.
Topics: 4-Chloromercuribenzenesulfonate; Anion Exchange Protein 1, Erythrocyte; Anion Transport Proteins; Ankyrins; Blood Proteins; Carrier Proteins; Cysteine; Erythrocyte Membrane; Ethylmaleimide; Glutathione; Humans; Iodoacetamide; Kinetics; Membrane Proteins; Molecular Weight; Oxidation-Reduction; Phenanthrolines | 1989 |
Accessibility of the N-ethylmaleimide-unreactive sulfhydryl of human erythrocyte Band 3.
Topics: Anion Exchange Protein 1, Erythrocyte; Chloromercuribenzoates; Chromatography, Affinity; Cysteine; Ethylmaleimide; Humans; p-Chloromercuribenzoic Acid | 1989 |
Chemical modification of chalcone isomerase by mercurials and tetrathionate. Evidence for a single cysteine residue in the active site.
Topics: Binding Sites; Catalysis; Cysteine; Enzyme Activation; Ethylmaleimide; Intramolecular Lyases; Isomerases; Kinetics; Mercuribenzoates; Mercuric Chloride; Mercury; Plant Proteins; Tetrathionic Acid; Thiosulfates; Tritium; Urea | 1989 |
Reactivities of sulfhydryl groups in native and metal-free aminoacylase I.
Topics: Amidohydrolases; Apoenzymes; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Kinetics; Metalloproteins; Protein Binding; Zinc | 1989 |
Mitochondrial nicotinamide nucleotide transhydrogenase: NADPH binding increases and NADP binding decreases the acidity and susceptibility to modification of cysteine-893.
Topics: Animals; Cattle; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; In Vitro Techniques; Kinetics; Mitochondria; NADH, NADPH Oxidoreductases; NADP; NADP Transhydrogenases; Substrate Specificity | 1989 |
Evidence for the existence of at least two different binding sites for 5HT-reuptake inhibitors within the 5HT-reuptake system from human platelets.
Topics: Arsenicals; Binding Sites; Blood Platelets; Carrier Proteins; Cysteine; Dithiothreitol; Ethylmaleimide; Humans; Imipramine; Neurotransmitter Uptake Inhibitors; Quinolines; Receptors, Drug; Receptors, Neurotransmitter; Serotonin | 1988 |
Exchange of cystine and glutamate across plasma membrane of human fibroblasts.
Topics: Amino Acids; Binding, Competitive; Biological Transport, Active; Cell Membrane; Cysteine; Cystine; Ethylmaleimide; Fibroblasts; Glutamates; Glutamic Acid; Humans | 1986 |
gamma-Glutamyl transpeptidase (gamma-GT) and maintenance of thiol pools in tumor cells resistant to alkylating agents.
Topics: Alkylating Agents; Animals; Cell Line; Cysteine; Drug Resistance; Ethylmaleimide; gamma-Glutamyltransferase; Glutamates; Kinetics; Leukemia L1210; Melphalan; Mice; Sulfhydryl Compounds | 1987 |
Inhibition by forskolin of excitatory amino acid-induced accumulation of cyclic AMP in guinea pig hippocampal slices.
Topics: Adenosine; Amino Acids; Animals; Aspartic Acid; Chlorides; Colforsin; Cyclic AMP; Cysteine; Drug Interactions; Ethylmaleimide; Glutamates; Glutamic Acid; Guanosine Triphosphate; Guinea Pigs; Hippocampus; Histamine; Male; N-Methylaspartate; Neurotransmitter Agents; Veratridine | 1988 |
Characteristics of histidine uptake by human placental microvillous membrane vesicles.
Topics: Aminoisobutyric Acids; Anions; Biological Transport, Active; Cations; Cysteine; Ethylmaleimide; Female; Gluconates; Glutamine; Histidine; Humans; Hydrogen-Ion Concentration; Microvilli; Osmolar Concentration; Phenylalanine; Placenta; Pregnancy; Sodium; Temperature | 1989 |
Analysis of biological thiols: determination of thiol components of disulfides and thioesters.
Topics: Animals; Bridged Bicyclo Compounds; Coenzyme A; Cysteine; Disulfides; Dithiothreitol; Esters; Ethylmaleimide; Glutathione; Hydroxylamine; Hydroxylamines; Male; Mitochondria, Liver; Neurospora crassa; Rats; Sulfhydryl Compounds | 1986 |
Reactive sulfhydryl groups of sarcoplasmic reticulum ATPase. III. Identification of cysteine residues whose modification with N-ethylmaleimide leads to loss of the Ca2+-transporting activity.
Topics: Animals; Binding Sites; Calcium; Calcium-Transporting ATPases; Cysteine; Ethylmaleimide; Kinetics; Muscles; Protein Binding; Rabbits; Sarcoplasmic Reticulum; Sulfhydryl Compounds | 1987 |
Evidence for a reactive cysteine at the nucleotide binding site of spinach ribulose-5-phosphate kinase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Ethanolamines; Ethylmaleimide; Kinetics; Nucleotides; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Plants | 1985 |
The reactive cysteine residue of pig kidney fructose 1,6-bisphosphatase is related to a fructose 2,6-bisphosphate allosteric site.
Topics: Animals; Binding Sites; Cysteine; Enzyme Activation; Ethylmaleimide; Fructose-Bisphosphatase; Fructosediphosphates; Hexosediphosphates; Kidney; Potassium; Swine | 1985 |
Interaction between bound cupric ion and spin-labeled cysteine beta-93 in human and horse hemoglobins.
Topics: Animals; Binding Sites; Copper; Cysteine; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Hemoglobins; Horses; Humans; Protein Binding; Species Specificity; Spin Labels | 1985 |
Microenvironment around the essential cysteine residues in chicken liver fructose-1,6-bisphosphatase as analyzed by ESR spin labelling.
Topics: Animals; Chickens; Cysteine; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Fructose-Bisphosphatase; Iodoacetates; Iodoacetic Acid; Liver; Protein Conformation; Spin Labels; Sulfhydryl Compounds | 1986 |
Activation of fructose-1,6-bisphosphatases by monovalent cations and its relationship with a fructose-2,6-bisphosphate allosteric site.
Topics: Animals; Binding Sites; Cyanates; Cysteine; Enzyme Activation; Ethylmaleimide; Fructose-Bisphosphatase; Fructosediphosphates; Hexosediphosphates; Kidney; Kinetics; Magnesium; Potassium; Swine | 1985 |
Chemical modification of the brown-fat-mitochondrial uncoupling protein with tetranitromethane and N-ethylmaleimide. A cysteine residue is implicated in the nucleotide regulation of anion permeability.
Topics: Adipose Tissue, Brown; Animals; Carrier Proteins; Chlorides; Cricetinae; Cysteine; Ethylmaleimide; Guanosine Diphosphate; Ion Channels; Male; Membrane Proteins; Mesocricetus; Methane; Mitochondria; Mitochondrial Proteins; Permeability; Protein Binding; Protons; Tetranitromethane; Uncoupling Protein 1 | 1986 |
Import of cytochrome c into mitochondria. Cytochrome c heme lyase.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cytochrome c Group; Ethylmaleimide; Heme; Hemin; Lyases; Mitochondria; Models, Molecular; NAD; NADP; Neurospora crassa; Peptide Fragments; Protein Conformation; Reticulocytes; Trypsin | 1987 |
Selective thiol group modification renders fructose-1,6-bisphosphatase insensitive to fructose 2,6-bisphosphate inhibition.
Topics: Animals; Cysteine; Ethylmaleimide; Fructose-Bisphosphatase; Fructosediphosphates; Hexosediphosphates; Kidney; Kinetics; Swine | 1987 |
Role of cysteine residues in glutathione synthetase from Escherichia coli B. Chemical modification and oligonucleotide site-directed mutagenesis.
Topics: Alanine; Amino Acids; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Codon; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Ethylmaleimide; Genes, Bacterial; Glutathione Synthase; Mutation; p-Chloromercuribenzoic Acid; Peptide Synthases; Plasmids; Sulfhydryl Compounds; Thiocyanates; Transformation, Bacterial | 1988 |
Structure of a bacterial sensory receptor. A site-directed sulfhydryl study.
Topics: Cell Membrane; Chemotaxis; Cysteine; Escherichia coli; Ethylmaleimide; Kinetics; Macromolecular Substances; Membrane Proteins; Mutation; Plasmids; Sensory Receptor Cells; Species Specificity; Sulfhydryl Compounds | 1988 |
Site-specific alteration of cysteine 281, cysteine 344, and cysteine 349 in the proline carrier of Escherichia coli.
Topics: Amino Acid Transport Systems, Neutral; Azetidines; Carrier Proteins; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Kinetics; Mutation; Plasmids; Proline; Protein Conformation; Symporters | 1988 |
Site-specific mutagenesis of cysteine 148 to serine in the lac permease of Escherichia coli.
Topics: Cysteine; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Genes; Genes, Bacterial; Kinetics; Membrane Transport Proteins; Molecular Weight; Monosaccharide Transport Proteins; Mutation; Nucleic Acid Heteroduplexes; Nucleic Acid Hybridization; Serine; Species Specificity; Symporters; Thiogalactosides | 1986 |
Fluorescence energy transfer between Cys-10 residues in F-actin filaments.
Topics: Actins; Animals; Cysteine; Energy Transfer; Ethylmaleimide; Fluorescent Dyes; Macromolecular Substances; Muscles; Naphthalenesulfonates; p-Dimethylaminoazobenzene; Rabbits; Spectrometry, Fluorescence; Spectrophotometry | 1986 |
Reactive sulfhydryl groups of alpha 39, a guanine nucleotide-binding protein from brain. Location and function.
Topics: Adenosine Diphosphate Ribose; Alkylation; Amino Acid Sequence; Animals; Brain; Cattle; Cysteine; Ethylmaleimide; GTP Phosphohydrolases; GTP-Binding Proteins; Peptide Fragments; Protein Conformation; Trypsin | 1987 |
Three possible disulfides in the acetylcholine receptor alpha-subunit.
Topics: Animals; Borohydrides; Bungarotoxins; Cysteine; Disulfides; Ethylmaleimide; Lysine; Macromolecular Substances; Maleimides; Peptide Mapping; Receptors, Cholinergic; Serine Endopeptidases; Torpedo | 1988 |
Reversible dissociation of active octamer of cyanase to inactive dimer promoted by alteration of the sulfhydryl group.
Topics: Aminohydrolases; Azides; Bicarbonates; Carbon-Nitrogen Lyases; Chromatography, Gel; Cysteine; Enzyme Activation; Enzyme Reactivators; Escherichia coli; Ethylmaleimide; Macromolecular Substances; Mercuric Chloride; Methyl Methanesulfonate; Mutation; Oxalates; Oxalic Acid; Serine; Spectrometry, Fluorescence; Structure-Activity Relationship; Sulfhydryl Compounds; Tetranitromethane | 1988 |
The central helix of calmodulin functions as a flexible tether.
Topics: Calmodulin; Cross-Linking Reagents; Crystallization; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Ethylmaleimide; Glutamine; Mutation; Myosin-Light-Chain Kinase; Protein Conformation; Structure-Activity Relationship; Threonine; Trypsin | 1988 |
Enzyme IIMtl of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: identification of the activity-linked cysteine on the mannitol carrier.
Topics: Chromatography, High Pressure Liquid; Cysteine; Escherichia coli; Ethylmaleimide; Mannitol; Oxidation-Reduction; Peptide Fragments; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphorylation; Structure-Activity Relationship | 1988 |
Conformational features of bovine heart mitochondrial transhydrogenase.
Topics: Animals; Binding Sites; Cattle; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; In Vitro Techniques; Mitochondria, Heart; NADH, NADPH Oxidoreductases; NADP; NADP Transhydrogenases; Protein Conformation; Sulfhydryl Reagents | 1988 |
Identification of the cysteine residue in the active site of horse liver mitochondrial aldehyde dehydrogenase.
Topics: Aldehyde Dehydrogenase; Amino Acid Sequence; Animals; Binding Sites; Chloral Hydrate; Chromatography, High Pressure Liquid; Cysteine; Ethylmaleimide; Horses; Kinetics; Mitochondria, Liver; Molecular Sequence Data; Peptide Mapping | 1988 |
Limited proteolysis of lactose permease from Escherichia coli.
Topics: Amino Acid Sequence; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Hydrolysis; Membrane Transport Proteins; Molecular Weight; Monosaccharide Transport Proteins; Octoxynol; Peptide Hydrolases; Polyethylene Glycols; Symporters | 1986 |
Site-specific alteration of cysteine 176 and cysteine 234 in the lactose carrier of Escherichia coli.
Topics: Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Hydroxymercuribenzoates; Kinetics; Lactose; Membrane Transport Proteins; Monosaccharide Transport Proteins; Mutation; Symporters | 1986 |
A sulfhydryl presumed essential is not required for catalysis by an aminoacyl-tRNA synthetase.
Topics: Alanine; Alkylation; Amino Acid Sequence; Amino Acyl-tRNA Synthetases; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Glutamine; Glycine-tRNA Ligase; Kinetics; Mutation | 1986 |
Identification of the essential cysteine residue of NADH-cytochrome b5 reductase.
Topics: Amino Acids; Animals; Cattle; Chromatography, Affinity; Chromatography, High Pressure Liquid; Cyanogen Bromide; Cysteine; Cytochrome Reductases; Cytochrome-B(5) Reductase; Ethylmaleimide; Liver; Male; NADH, NADPH Oxidoreductases | 1986 |
Labeling of functionally sensitive sulfhydryl-containing domains of acetylcholine receptor from Torpedo californica membranes.
Topics: Alkylation; Animals; Binding, Competitive; Bungarotoxins; Carbachol; Cell Membrane; Cell Membrane Permeability; Cysteine; Electric Organ; Ethylmaleimide; Fluorescent Dyes; Humans; Hydrogen-Ion Concentration; Maleimides; Receptors, Cholinergic; Spectrometry, Fluorescence; Sulfhydryl Compounds; Sulfhydryl Reagents; Torpedo | 1986 |
Selective labelling of Cys-10 on actin.
Topics: Actins; Animals; Cattle; Cysteine; Ethylmaleimide; Fluoresceins; Magnetic Resonance Spectroscopy; Muscles; Peptide Fragments; Protein Conformation; Rabbits; Spectrometry, Fluorescence; Thrombin; Viscosity | 1986 |
Site-directed mutagenesis of cysteine-148 in the lac permease of Escherichia coli: effect on transport, binding, and sulfhydryl inactivation.
Topics: Amino Acid Sequence; Binding Sites; Biological Transport, Active; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Genes, Bacterial; Hydrogen-Ion Concentration; Kinetics; Lactose; Membrane Transport Modulators; Membrane Transport Proteins; Monosaccharide Transport Proteins; Mutation; Sulfhydryl Reagents; Symporters | 1985 |
The phosphoenolpyruvate-dependent fructose-specific phosphotransferase system in Rhodopseudomonas sphaeroides. EIIFru possesses a Zn2+-binding site and a dithiol/disulfide redox centre.
Topics: Cations, Divalent; Cysteine; Disulfides; Ethylmaleimide; Fructose; Hydrogen-Ion Concentration; Oxidation-Reduction; Phosphoenolpyruvate Sugar Phosphotransferase System; Protein Conformation; Rhodopseudomonas; Zinc | 1986 |
Modification of the calmodulin-stimulated phosphatase, calcineurin, by sulfhydryl reagents.
Topics: Animals; Binding Sites; Calcium; Calmodulin-Binding Proteins; Cattle; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Macromolecular Substances; Manganese; Nickel; Sulfhydryl Reagents | 1986 |
An essential cysteine residue located in the vicinity of the FAD-binding site in short-chain, medium-chain, and long-chain acyl-CoA dehydrogenases from rat liver mitochondria.
Topics: Acyl-CoA Dehydrogenase, Long-Chain; Amino Acids; Animals; Binding Sites; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Flavin-Adenine Dinucleotide; Hydrogen-Ion Concentration; Hydroxymercuribenzoates; Kinetics; Methylmercury Compounds; Mitochondria, Liver; p-Chloromercuribenzoic Acid; Rats; Spectrophotometry | 1985 |
Mechanism of electron transfer to coordinated dioxygen of oxyhemoglobins to yield peroxide and methemoglobin. Protein control of electron donation by aquopentacyanoferrate(II).
Topics: Alkylation; Catalase; Cysteine; Electron Transport; Ethylmaleimide; Ferrocyanides; Humans; Hydrogen Peroxide; Methemoglobin; Oxygen; Oxyhemoglobins; Sodium Chloride; Zinc | 1985 |
The topology of the major band 4.5 protein component of the human erythrocyte membrane: characterization of reactive cysteine residues.
Topics: Blood Proteins; Cysteine; Cytochalasin B; Erythrocyte Membrane; Ethylmaleimide; Humans; Kinetics; Membrane Proteins; Monosaccharide Transport Proteins; Nucleoside Transport Proteins; Protein Conformation; Trypsin | 1985 |
Sequence of the N-terminal formic acid fragment and location of the N-ethylmaleimide-binding site of the phosphate transport protein from beef heart mitochondria.
Topics: Amino Acid Sequence; Animals; Carrier Proteins; Cattle; Cyanogen Bromide; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Fluorometry; Formates; Mitochondria, Heart; Mitochondrial ADP, ATP Translocases; Peptide Fragments; Phosphate-Binding Proteins; Silver | 1985 |
Mechanism of the inhibitory effects of cardiac glycosides on lipolysis.
Topics: Amides; Animals; Cardiac Glycosides; Cyclic AMP; Cysteine; Dicumarol; Digitoxin; Dinitrophenols; Ethylmaleimide; Glutathione; Glycerol; Hydrolysis; In Vitro Techniques; Iodoacetates; Lipid Metabolism; Lipid Mobilization; Male; Norepinephrine; Ouabain; Rats; Rats, Inbred Strains; Sulfhydryl Compounds; Theophylline | 1972 |
The nature of silver binding in the canine thyroid "C" cell.
Topics: Acetylation; Animals; Binding Sites; Calcitonin; Cysteine; Cystine; Disulfides; Dogs; Ethylmaleimide; Hydrogen-Ion Concentration; Hydroxides; Methionine; Oxidation-Reduction; Periodic Acid; Polysaccharides; Potassium Permanganate; Silver; Staining and Labeling; Sulfonic Acids; Thyroid Gland | 1973 |
Studies on a possible simultaneous differential demonstration of SH groups and S-S bonds.
Topics: Animals; Cysteine; Cystine; Disulfides; Ethylmaleimide; Evaluation Studies as Topic; Histocytochemistry; Homocysteine; Hydrogen-Ion Concentration; Indicators and Reagents; Liver; Methionine; Methods; Mice; Nerve Tissue; Staining and Labeling; Sulfhydryl Compounds | 1973 |
Tumor growth and disulfide reduction: possible dependence on protein-disulfide reductase.
Topics: Animals; Antigens, Neoplasm; Arsenic; Ascitic Fluid; Cell Survival; Cell-Free System; Cysteine; Disulfides; Dithiothreitol; Edetic Acid; Ethylmaleimide; Glutathione; Glutathione Reductase; Hot Temperature; Iodoacetates; Mice; NADH, NADPH Oxidoreductases; Neoplasm Transplantation; Neoplasms, Experimental; Oxidation-Reduction; Transplantation, Homologous | 1973 |
Competitive radioprotection and radiosensitization in chemical systems.
Topics: Acetophenones; Ascorbic Acid; Binding, Competitive; Cyclic N-Oxides; Cysteamine; Cysteine; Dithiothreitol; DNA; Ethylmaleimide; Histamine; Metronidazole; NAD; NADP; Nitro Compounds; Nitrobenzenes; Nitrofurantoin; Nitrofurazone; Nitrophenols; Oxygen; Paraquat; Promethazine; Pyridinium Compounds; Quinones; Radiation Effects; Radiation-Protective Agents; Radiation-Sensitizing Agents; Tetrazolium Salts; Vitamin K | 1974 |
[Functional importance of sulfhydryl groups in membranes of lysosomal granules of bovine polymorphonuclear leukocytes].
Topics: Acid Phosphatase; Amides; Animals; Cathepsins; Cattle; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Lysosomes; Membranes; Microscopy, Electron; Neutrophils; Organomercury Compounds; Peptides; Photometry; Sulfhydryl Compounds | 1968 |
On the active site of myosin A-adenosine triphosphatase. IX. Chemical modification of the enzyme by monoiodoacetamide.
Topics: Adenosine Triphosphatases; Amides; Animals; Binding Sites; Chloromercuribenzoates; Chromatography; Cysteine; Dextrans; Edetic Acid; Enzyme Activation; Ethylmaleimide; Histidine; Iodoacetates; Lysine; Muscle Proteins; Nitrobenzenes; Rabbits; Sulfonamides; Sulfonic Acids; Toluene; Tyrosine | 1970 |
The effect of a cross-bridging thiol reagent on the catecholamine fluxes of adrenal medulla vesicles.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Adrenal Medulla; Animals; Carbon Isotopes; Catecholamines; Cattle; Cysteine; Epinephrine; Ethers; Ethylmaleimide; In Vitro Techniques; Membranes; Pyrroles; Sulfhydryl Compounds | 1970 |
Studies on the mechanism of the conversion of coupling factor 1 from chloroplasts to an active adenosine triphosphatase.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Benzoates; Catalysis; Chemical Phenomena; Chemistry; Chloroplasts; Chromatography, Gel; Cysteine; Cystine; Detergents; Enzyme Activation; Ethylmaleimide; Hot Temperature; Iodoacetates; Nucleotides; Oxidation-Reduction; Plant Cells; Sulfhydryl Compounds; Sulfides; Sulfonic Acids; Trypsin; Urea | 1970 |
Plasmin digestion of stabilized and non-stabilized fibrin illustrated by pH-stat titration and thrombelastography.
Topics: Cysteine; Ethylmaleimide; Fibrinolysin; Fibrinolysis; Glycine; Humans; In Vitro Techniques; Iodoacetates; Isoniazid; Thrombelastography; Trypsin | 1971 |
[Fractionation of platelet homogenates. II. Determination and properties of structure-bound adenine nucleotides].
Topics: Adenine Nucleotides; Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Azides; Blood Platelets; Calcium; Chlorpromazine; Cysteine; Dinitrophenols; Edetic Acid; Ethylmaleimide; In Vitro Techniques; Iodoacetates; Lipase; Muramidase; Organoids; Organomercury Compounds; Osmotic Fragility; Ouabain; Phenoxybenzamine; Phospholipases; Potassium; Prenylamine; Reserpine; Sodium; Surface-Active Agents; Swine; Thrombin; Trypsin; Tyramine | 1970 |
Partial purification and characterization of an ADP phosphohydrolase from human plasma.
Topics: Adenine Nucleotides; Adenosine Triphosphatases; Calcium; Carbon Isotopes; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Ion Exchange; Cyanides; Cysteine; Cytosine Nucleotides; Edetic Acid; Ethylmaleimide; Guanine Nucleotides; Humans; Hydrogen-Ion Concentration; Inosine Nucleotides; Kinetics; Magnesium; Manganese; Nucleotidases; Plasma; Tartrates; Tritium; Uracil Nucleotides; Zinc | 1971 |
A sulfhydryl group is not the covalent catalyst in the thymidylate synthetase reaction.
Topics: Benzoates; Binding Sites; Carbon Radioisotopes; Chromatography, Gel; Chromatography, Thin Layer; Cysteine; Disulfides; Ethylmaleimide; Floxuridine; Methyltransferases; Nitro Compounds; Protein Binding; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Thymidylate Synthase; Uracil Nucleotides | 1974 |
Effect of N-ethylmaleimide and of heat treatment on the binding of dynein to ethylenediaminetetraacetic acid extracted axonemes.
Topics: Adenosine Triphosphatases; Animals; Binding Sites; Chromosomes; Cilia; Cysteine; Edetic Acid; Ethylmaleimide; Hot Temperature; Protein Binding; Proteins; Tetrahymena pyriformis; Time Factors | 1974 |
Inactivation of yeast alcohol dehydrogenase by N-alkylmaleimides.
Topics: Alcohol Oxidoreductases; Alkylation; Chemical Phenomena; Chemistry; Cysteine; Ethylmaleimide; Glutathione; Hydrogen-Ion Concentration; Imides; Kinetics; NAD; Niacinamide; Saccharomyces; Temperature | 1968 |
Biosynthesis of triphosphoinositide in rat kidney cortex.
Topics: Adenine Nucleotides; Adenosine Triphosphate; Ammonium Chloride; Animals; Cell Membrane; Chloromercuribenzoates; Copper; Cysteine; Detergents; Edetic Acid; Ethylmaleimide; Glutathione; Kidney; Kinetics; Lithium; Magnesium; Male; Mercury; Microscopy, Electron; Phosphatidylinositols; Phosphorus Isotopes; Phosphotransferases; Potassium Chloride; Rats; Sodium Chloride; Sulfhydryl Compounds | 1970 |
Purification and properties of adenyl cyclase from Streptococcus salivarius.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Adenylyl Cyclase Inhibitors; Adenylyl Cyclases; Benzoates; Binding Sites; Chemical Precipitation; Chromatography, Gel; Cyclic AMP; Cyclic GMP; Cysteine; Deoxyribonucleases; Drug Stability; Electrophoresis, Disc; Enzyme Activation; Enzymes; Ethylmaleimide; Fluorides; Hydrogen-Ion Concentration; Kinetics; Magnesium; Manganese; Mercury; Organometallic Compounds; Phosphoric Monoester Hydrolases; Phosphotransferases; Pyrophosphatases; Quaternary Ammonium Compounds; Streptococcus; Sulfates; Sulfhydryl Reagents; Thermodynamics | 1971 |
ESR-studies of the mechanism of radiosensitization. Action of N-ethylmaleimide on irradiated sulfhydryl compounds, irradiated DNA, and irradiated nucleotides.
Topics: Adenine Nucleotides; Cyclic AMP; Cystamine; Cysteine; DNA; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Free Radicals; Nucleotides; Radiation-Sensitizing Agents; Sulfhydryl Compounds; Thymidine; Thymine | 1971 |
Selective chemical modification of malate dehydrogenase. N-ethylmaleimide modification of active center sulfhydryl residues.
Topics: Amino Acids; Ammonium Sulfate; Animals; Binding Sites; Carbon Isotopes; Chemical Phenomena; Chemical Precipitation; Chemistry; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Cysteine; Electrophoresis; Ethylmaleimide; Hydrogen-Ion Concentration; Imides; Kinetics; Malate Dehydrogenase; Mathematics; Mitochondria, Muscle; Myocardium; NAD; Protein Binding; Spectrophotometry; Succinates; Swine; Ultracentrifugation | 1971 |
The effect N-ethylmaleimide on peptide hormone lacking a disulfide bridge.
Topics: Adipose Tissue; Adrenocorticotropic Hormone; Animals; Cyclic AMP; Cysteine; Ethylmaleimide; Glucose; Lipid Metabolism; Melanocyte-Stimulating Hormones; Parathyroid Hormone; Rats | 1971 |
Biological activity of diazonium compounds. Studies on the mechanism of action of 4 (or 5)-diazoimidazole-5 (or 4)-carboxamide on 5-hydroxytryptamine release from rabbit platelets. II. Comparative studies with N-ethylmaleimide in vitro.
Topics: Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Amides; Animals; Blood Platelets; Calcium; Carboxylic Acids; Cysteine; Diazonium Compounds; Diphosphates; Ethylmaleimide; Glutathione; Imidazoles; Phosphocreatine; Rabbits; Serotonin; Serotonin Antagonists; Sulfhydryl Compounds | 1971 |
One-electron transfer reactions in biochemical systems. VI. Changes in electron transfer mechanism of lipoamide dehydrogenase by modification of sulfhydryl groups.
Topics: Animals; Arsenic; Catalysis; Copper; Cysteine; Dihydrolipoamide Dehydrogenase; Electron Spin Resonance Spectroscopy; Electron Transport; Ethylmaleimide; Ferricyanides; Hydrogen-Ion Concentration; Kinetics; Mercury; Models, Chemical; Myocardium; NAD; Organometallic Compounds; Oxidation-Reduction; Quinones; Spectrophotometry; Swine | 1972 |
Pharmacologic receptor activity of rabbit aorta. Effect of dithiothreitol and N-ethylmaleimide.
Topics: Amine Oxidase (Copper-Containing); Angiotensin II; Animals; Aorta; Cysteine; Cystine; Dithiothreitol; Erythritol; Ethylmaleimide; Histamine; In Vitro Techniques; Male; Methyltransferases; Muscle Contraction; Muscle, Smooth; Norepinephrine; Phentolamine; Potassium Chloride; Rabbits; Receptors, Drug; Serotonin | 1973 |
Some properties of the hydro-osmotic vasopressin "effector": interactions with N-ethylmaleimide.
Topics: Animals; Bufo marinus; Calcium; Cell Membrane Permeability; Culture Media; Cyclic AMP; Cysteine; Ethylmaleimide; Hypertonic Solutions; In Vitro Techniques; Mannitol; Osmolar Concentration; Osmosis; Urinary Bladder; Vasopressins; Zinc | 1973 |
Virocidal cleavage of disulphide bonds within structural proteins of Sendai virus.
Topics: Acetamides; Animals; Carbon Radioisotopes; Centrifugation, Density Gradient; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Hemagglutination Inhibition Tests; Hemagglutinins, Viral; Hemolysin Proteins; Hemolysis; Immune Sera; Mercaptoethanol; Microscopy, Electron; Neuraminidase; Neutralization Tests; Parainfluenza Virus 1, Human; Peptides; Protein Denaturation; Rabbits; Viral Proteins | 1973 |
Isoleucyl transfer ribonucleic acid synthetase from Escherichia coli. Effect of limited cleavage by trypsin on activity and structure.
Topics: Adenosine Triphosphate; Amino Acids; Amino Acyl-tRNA Synthetases; Binding Sites; Carbon Radioisotopes; Catalysis; Chemical Phenomena; Chemistry; Chromatography, Gel; Cysteine; Diphosphates; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethylmaleimide; Hydrolysis; Isoleucine; Molecular Weight; Peptide Fragments; Phosphorus Radioisotopes; Sodium Dodecyl Sulfate; Transfer RNA Aminoacylation; Trypsin; Ultracentrifugation | 1974 |
Disulphide groups of gonadotropin receptors are essential for specific binding of human chorionic gonadotropin.
Topics: Animals; Binding Sites; Centrifugation, Density Gradient; Chorionic Gonadotropin; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Female; Humans; Iodine Radioisotopes; Male; Methylene Blue; Oxygen; Protein Binding; Receptors, Cell Surface; Testis | 1974 |
Functional groups of diphosphopyridine nucleotide-linked isocitrate dehydrogenase from bovine heart. Studies of cysteine residues.
Topics: Amino Acids; Animals; Benzoates; Binding Sites; Cattle; Chloromercuribenzoates; Cyanides; Cysteine; Dithiothreitol; Ethylmaleimide; Hydrogen-Ion Concentration; Iodoacetates; Isocitrate Dehydrogenase; Kinetics; Mercaptoethanol; Molecular Weight; Myocardium; NAD; Nitro Compounds; Oxidation-Reduction; Protein Binding; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Temperature; Thiosulfates; Time Factors | 1974 |
Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. III. Isolation and sequences of peptides containing selectively labeled lysine and cysteine residues.
Topics: Amino Acid Sequence; Amino Acids; Aminopeptidases; Binding Sites; Borohydrides; Carbon Radioisotopes; Carboxypeptidases; Chromatography, Gel; Chromatography, Ion Exchange; Chromatography, Paper; Chymotrypsin; Cysteine; Electrophoresis, Paper; Ethylmaleimide; Glutamate Dehydrogenase; Leucyl Aminopeptidase; Lysine; NAD; Neurospora; Neurospora crassa; Oxidation-Reduction; Papain; Peptide Fragments; Protein Binding; Pyridoxal Phosphate; Thermolysin; Trypsin | 1974 |
Effect of reagents affecting specific groups of proteins on the haemagglutinating activity of rubella virus.
Topics: Anhydrides; Aniline Compounds; Antigens; Benzoates; Copper; Cysteine; Ethylmaleimide; Hemagglutination Tests; Hemagglutinins, Viral; Iodoacetates; Mercaptoethanol; Rubella virus; Sulfonic Acids; Viral Proteins | 1969 |
Lipid peroxide formation in microsomes. General considerations.
Topics: Animals; Ascorbic Acid; Bile Acids and Salts; Chloromercuribenzoates; Cysteine; Electron Transport; Ethylmaleimide; Ferredoxins; Ferricyanides; Glutathione; In Vitro Techniques; Lipid Metabolism; Liver; Male; Metals; Microsomes; NADP; Oxygen Consumption; Peroxides; Rats | 1969 |
Lipid peroxide formation in microsomes. Relationship of hydroxylation to lipid peroxide formation.
Topics: Aminopyrine; Animals; Carbon Tetrachloride; Chloroform; Chloromercuribenzoates; Codeine; Cysteine; Ethylmaleimide; Glutathione; In Vitro Techniques; Lipid Metabolism; Microsomes; NAD; NADP; Oxidation-Reduction; Peroxides; Rats | 1969 |
The role of sulfhydryl groups in the catalytic function of isocitrate dehydrogenase. 3. Effect of N-ethylmaleimide on chemical and physical properties.
Topics: Amino Acids; Animals; Antigens; Benzoates; Binding Sites; Carbon Isotopes; Chemical Phenomena; Chemistry; Chromatography, Gel; Chromatography, Paper; Cysteine; Ethylmaleimide; Immune Sera; Immunodiffusion; Isocitrate Dehydrogenase; Kinetics; Manganese; Methionine; Myocardium; NADP; Optical Rotatory Dispersion; Peptide Hydrolases; Protein Denaturation; Rabbits; Succinates; Sulfhydryl Compounds; Sulfides; Swine; Time Factors | 1970 |
Sulfhydril groups and the concerted inhibition of NADP + -linked isocitrate dehydrogenase.
Topics: Animals; Binding Sites; Carboxy-Lyases; Cysteine; Drug Synergism; Ethylmaleimide; Glyoxylates; Isocitrate Dehydrogenase; Ketoglutaric Acids; Myocardium; NADP; Oxaloacetates; Oxidation-Reduction; Radiation Effects; Structure-Activity Relationship; Sulfhydryl Compounds; Swine | 1972 |
Studies on pharmacodynamic action of N-ethylmaleimide (NEM). l. Influence of NEM on synaptic transmission of sympathetic nerves in guinea pigs.
Topics: Animals; Anura; Calcium; Cysteine; Ethylmaleimide; Guanethidine; Guinea Pigs; Heart Atria; In Vitro Techniques; Male; Norepinephrine; Propranolol; Reserpine; Spinal Nerves; Sympathetic Nervous System; Synapses; Vas Deferens | 1973 |
Studies on pharmacodynamic action of N-ethylmaleimide (NEM). 2. An inflammatory property of NEM in rats.
Topics: Animals; Ascites; Capillary Permeability; Cortisone; Cysteine; Edema; Ethylmaleimide; Inflammation; Injections, Intraperitoneal; Injections, Subcutaneous; Mesentery; Rats | 1973 |
Mapping of Escherichia coli ribosomal components involved in peptidyl transferase activity.
Topics: Acetamides; Acyltransferases; Alkylation; Anti-Bacterial Agents; Bacterial Proteins; Binding, Competitive; Bromine; Carbon Isotopes; Chloramphenicol; Cysteine; Escherichia coli; Ethylmaleimide; Methionine; Methods; Peptide Elongation Factors; Peptides; Phenethylamines; Phenylalanine; Ribosomes; Succinimides | 1973 |
The role of sulfhydryl groups in human neutrophil adhesion, movement and particle ingestion.
Topics: Buffers; Cell Adhesion; Cell Movement; Chemotaxis; Chlorides; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Humans; Latex; Mercury; Micropore Filters; Microscopy, Phase-Contrast; Microspheres; Neutrophils; Phagocytosis; Plasma; Platelet Adhesiveness; Pseudopodia; Stereoisomerism; Sulfhydryl Compounds; Sulfhydryl Reagents | 1973 |
Structural studies on isoleucyl-tRNA synthetase from E. coli--identification of the cysteine residue modified specifically with N-ethylmaleimide.
Topics: Amino Acid Sequence; Amino Acids; Amino Acyl-tRNA Synthetases; Binding Sites; Carbon Radioisotopes; Chromatography, Gel; Chymotrypsin; Cysteine; Escherichia coli; Ethylmaleimide; Isoleucine; Peptide Fragments; Protein Binding; Protein Conformation; Trypsin | 1974 |
The effect of thiol-combining agents on polypeptide synthesis in cell-free system from Escherichia coli.
Topics: Anthraquinones; Bacterial Proteins; Carbon Radioisotopes; Cell-Free System; Cysteine; Escherichia coli; Ethylmaleimide; Peptide Biosynthesis; Peptide Elongation Factors; Phenylalanine; Phosphorus Radioisotopes; Puromycin; Ribosomes; Sulfhydryl Reagents; Thiocyanates | 1974 |
Some chemical and biological characteristics of showdomycin.
Topics: Antibiotics, Antineoplastic; Cysteine; Escherichia coli; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Maleimides; Mercaptoethylamines; Showdomycin; Sulfhydryl Compounds; Temperature | 1974 |
Syncatalytic modification of cytoplasmic aspartate aminotransferase: identification of a peptide containing the modified cysteinyl residue.
Topics: Amino Acids; Animals; Aspartate Aminotransferases; Benzoates; Carbon Isotopes; Chemical Phenomena; Chemistry; Chromatography, Gel; Chromatography, Ion Exchange; Cyanides; Cyanogen Bromide; Cysteine; Cytoplasm; Ethylmaleimide; Macromolecular Substances; Myocardium; Peptides; Sulfhydryl Compounds; Sulfides; Swine; Trypsin | 1972 |
Isolation of a cysteine-peptide at the active site of histidine ammonia-lyase.
Topics: Alkylation; Amino Acids; Ammonia-Lyases; Benzoates; Binding Sites; Carbon Isotopes; Chromatography, Gel; Chromatography, Paper; Cysteine; Disulfides; Electrophoresis, Paper; Ethylmaleimide; Histidine; Iodoacetates; Kinetics; Macromolecular Substances; Molecular Weight; Nitro Compounds; Oxidation-Reduction; Peptides; Pseudomonas; Trypsin | 1972 |
Selective cysteine modification in glyceraldehyde-3-phosphate dehydrogenase.
Topics: Acetone; Alkylation; Amino Acids; Animals; Benzoates; Binding Sites; Bromine; Carbon Isotopes; Chemical Phenomena; Chemistry; Chromatography, Paper; Cysteine; Dithiothreitol; Electrophoresis, Paper; Enzyme Activation; Ethylmaleimide; Glyceraldehyde-3-Phosphate Dehydrogenases; Iodine; Iodoacetates; Mercaptoethanol; Muscles; Papain; Peptides; Rabbits; Thiosulfates; Trypsin | 1972 |
[Pharmacological actions of N-ethylmaleimide (NEM). 1. Effects of NEM on sympathetic synapses in guinea pigs].
Topics: Acetylcholine; Animals; Anura; Calcium; Cocaine; Cysteine; Electric Stimulation; Ethylmaleimide; Guanethidine; Guinea Pigs; Heart Atria; Heart Rate; Hypogastric Plexus; In Vitro Techniques; Male; Muscle Contraction; Nicotine; Potassium; Propranolol; Reserpine; Spinal Cord; Sympathetic Nervous System; Synapses; Vas Deferens | 1972 |
The role of sulfhydryl groups in three models of inflammatory disease.
Topics: Animals; Arthritis; Carrageenan; Croton Oil; Cyclophosphamide; Cysteine; Dexamethasone; Disulfides; Edema; Ethylmaleimide; Freund's Adjuvant; Glycoproteins; Granuloma; Indomethacin; Male; Penicillamine; Rats; Sulfhydryl Compounds | 1973 |
The SH group of yeast phosphoglycerate kinase.
Topics: Acetamides; Adenosine Triphosphate; Benzoates; Binding Sites; Chemical Phenomena; Chemistry; Cysteine; Disulfides; Drug Stability; Ethylmaleimide; Glyceric Acids; Hydroxymercuribenzoates; Iodoacetates; Kinetics; Magnesium; Nitro Compounds; Organophosphorus Compounds; Phosphoglycerate Kinase; Yeasts; Zinc | 1973 |
Human placental 17 -oestradiol dehydrogenase. Sequence of a tryptic peptide containing an essential cysteine.
Topics: Alkylation; Amino Acids; Carbon Isotopes; Chromatography, Gel; Chromatography, Paper; Cysteine; Electrophoresis, Paper; Ethylmaleimide; Female; Humans; Hydroxysteroid Dehydrogenases; Iodoacetates; Peptides; Placenta; Pregnancy; Trypsin | 1973 |
Modification of three enzymes of the beta-ketoadipate pathway by N-methyl-N'-nitro-N-nitrosoguanidine.
Topics: Adipates; Binding Sites; Cell-Free System; Cysteine; Esterases; Ethylmaleimide; Half-Life; Hydrolases; Isomerases; Keto Acids; Kinetics; Lactones; Mercuribenzoates; Mercury; Nitro Compounds; Nitrosoguanidines; Protein Binding; Pseudomonas; Spectrophotometry; Time Factors | 1973 |
Reaction of cytoplasmic aspartate aminotransferase with tetranitromethane.
Topics: Animals; Aspartate Aminotransferases; Binding Sites; Carbon Isotopes; Chromatography, Gel; Cyanides; Cysteine; Cytosol; Ethylmaleimide; Methane; Myocardium; Nitro Compounds; Protein Binding; Protein Denaturation; Spectrophotometry; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Swine; Time Factors; Tyrosine | 1973 |
Induction of starfish oocyte maturation by disulfide-reducing agents.
Topics: Adenine; Amides; Animals; Chloromercuribenzoates; Cysteine; Dimercaprol; Disulfides; Dithiothreitol; Ethylmaleimide; Female; Fertilization; Glutathione; Insemination; Iodides; Iodoacetates; Iodobenzoates; Larva; Male; Ovum; Peroxides; Starfish; Time Factors | 1973 |
[Studies on the formation of mixed disulfides between D-penicillamine and serum proteins (author's transl)].
Topics: Animals; Blood Proteins; Cysteine; Cystine; Disulfides; Ethylmaleimide; Male; Penicillamine; Protein Binding; Rats; Serum Albumin | 1973 |
Inhibition of papain by N-ethylmaleimide.
Topics: Arginine; Benzoates; Binding Sites; Cysteine; Drug Stability; Enzyme Activation; Ethylmaleimide; Glycine; Kinetics; Mathematics; Mercury; Models, Chemical; Papain; Protein Binding; Spectrophotometry; Structure-Activity Relationship; Sulfhydryl Compounds | 1974 |
Bacterial luciferase. Chemistry of the reactive sulfhydryl.
Topics: Alkylating Agents; Amides; Bacteria; Binding Sites; Carbon Radioisotopes; Catalysis; Cysteine; Ethylmaleimide; Flavin Mononucleotide; Hydrogen-Ion Concentration; Iodoacetates; Kinetics; Luciferases; Luminescent Measurements; Macromolecular Substances; Mathematics; Models, Chemical; Organophosphorus Compounds; Oxidation-Reduction; Protein Binding; Structure-Activity Relationship; Sulfhydryl Compounds | 1974 |
The reactive sulfhydryl groups of cytoplasmic aspartate aminotransferase. Localization in the primary structure and spectral properties of their mixed disulfide derivatives with N-dansylcysteine.
Topics: Amino Acids; Animals; Aspartate Aminotransferases; Benzoates; Carbon Radioisotopes; Chromatography, Gel; Chromatography, Ion Exchange; Cyanogen Bromide; Cysteine; Cytoplasm; Dansyl Compounds; Disulfides; Electrophoresis, Paper; Ethylmaleimide; Methane; Myocardium; Nitro Compounds; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Swine | 1974 |
Physicochemical and catalytic properties of aspartate aminotransferase selectively modified at the tyrosine and cysteine residues.
Topics: Animals; Apoproteins; Aspartate Aminotransferases; Binding Sites; Carbon Radioisotopes; Cattle; Chemical Phenomena; Chemistry; Chromatography, Gel; Chromatography, Ion Exchange; Circular Dichroism; Cysteine; Electrophoresis, Paper; Enzyme Activation; Ethylmaleimide; Ketoglutarate Dehydrogenase Complex; Methane; Myocardium; Nitro Compounds; Protein Binding; Protein Conformation; Pyridoxal Phosphate; Spectrophotometry; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Tyrosine | 1974 |
Intracellular concentration of cysteine in Escherichia coli and its relation to repression of the sulphate-activating enzymes.
Topics: Adenine Nucleotides; Carbon Isotopes; Chromatography, Paper; Cysteine; Enzyme Repression; Escherichia coli; Ethylmaleimide; Glutathione; Sulfates; Sulfur Isotopes; Time Factors | 1967 |
Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli.
Topics: Bacterial Proteins; Binding Sites; Biological Transport, Active; Carbohydrates; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Formaldehyde; Galactose; Glycosides; Lactose | 1968 |
The use of N-ethylmaleimide in stabilizing and measuring inorganic sulphur compounds.
Topics: Cysteine; Escherichia coli; Ethylmaleimide; Methods; Oxidoreductases; Sulfides; Sulfites; Sulfur; Sulfur Isotopes | 1968 |
Substrate binding properties of mutant and wild-type A proteins of Escherichia coli tryptophan synthetase.
Topics: Amino Acid Sequence; Bacterial Proteins; Binding Sites; Chemical Phenomena; Chemistry; Cysteine; Escherichia coli; Ethylmaleimide; Genetics, Microbial; Glycerophosphates; Hydro-Lyases; Iodoacetates; Mutation; Operon; Protein Binding; Tryptophan | 1967 |
Structural and functional roles of the cysteine residues in the alpha subunit of the Escherichia coli trypotphan synthetase. II. Functional roles of the cysteine residues.
Topics: Benzoates; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry; Chromatography, Gel; Cysteine; Dextrans; Escherichia coli; Ethylmaleimide; Hydro-Lyases; Mercury; Mutation; Nitrobenzenes; Peptides; Tryptophan | 1969 |
Comparative structural studies of the active site of ATP-guanidine phosphotransferases. The essential cysteine tryptic peptide of arginine kinase from Homarus vulgaris muscle.
Topics: Amino Acid Sequence; Animals; Arginine; Binding Sites; Carbon Isotopes; Carboxypeptidases; Chromatography, Gel; Chromatography, Ion Exchange; Chromatography, Paper; Chymotrypsin; Creatine Kinase; Crustacea; Cysteine; Electrophoresis; Ethylmaleimide; Leucyl Aminopeptidase; Muscles; Paper; Pepsin A; Peptides; Phosphotransferases; Trypsin | 1969 |
The role of sulfhydryl groups in the activity of type A botulinum toxin.
Topics: Antitoxins; Benzoates; Binding Sites; Chemical Phenomena; Chemistry; Clostridium botulinum; Crystallization; Cysteine; Cystine; Ethylmaleimide; Iodoacetates; Mercury; Molecular Weight; Organometallic Compounds; Sulfhydryl Compounds; Sulfides; Toxins, Biological | 1970 |
Chemical evidence showing involvement of sulfhydryl groups in protein synthesis with E. coli system.
Topics: Bacterial Proteins; Benzimidazoles; Carbon Isotopes; Cell-Free System; Cysteine; Depression, Chemical; Escherichia coli; Ethylmaleimide; Fluorescence; Imides; Metabolism; Peptide Biosynthesis; Phenylalanine; Polynucleotides; Pyrroles; Ribosomes; RNA, Transfer; Spectrophotometry; Succinates; Sulfhydryl Compounds; Sulfhydryl Reagents; Ultraviolet Rays; Uracil Nucleotides | 1971 |
Effect of sulfhydryl-binding reagents on islet tissue permeability: protection and reversal by thiol compounds.
Topics: Alloxan; Animals; Arsenicals; Cadmium; Carbon Isotopes; Cell Membrane Permeability; Cysteine; Dimercaprol; Drug Antagonism; Ethylmaleimide; Fishes; Glutathione; In Vitro Techniques; Iodoacetates; Islets of Langerhans; Mannitol; Sulfhydryl Compounds; Sulfhydryl Reagents | 1971 |
The effect of sulphydryl and enzyme inhibitors on platelet aggregation in vitro.
Topics: Adenine Nucleotides; Adenosine Triphosphate; Blood Coagulation; Blood Platelets; Cyanides; Cysteine; Densitometry; Depression, Chemical; Edetic Acid; Enzyme Inhibitors; Ethylmaleimide; Fluorides; Fluoroacetates; Glycolysis; Humans; Mercury; Sulfhydryl Compounds | 1966 |
Acid-catalysed conversion of triethyleneimine thiophosphoramide (thio-TEPA) to an SH compound.
Topics: Acids; Benzoates; Catalysis; Chromatography, Thin Layer; Cysteamine; Cysteine; Ethylenes; Ethylmaleimide; Hydrogen-Ion Concentration; Imines; Nitrobenzenes; Phenols; Pyridines; Sulfhydryl Compounds; Sulfur; Thiotepa | 1968 |
Protective factors in the detoxication of hydrogen peroxide in erythrocytes.
Topics: Animals; Azides; Carbon Isotopes; Catalase; Cats; Cattle; Cysteine; Dogs; Erythrocytes; Ethylmaleimide; Glucose; Glutathione; Goats; Guinea Pigs; Haplorhini; Hemoglobins; Hexosephosphates; Humans; Hydrogen Peroxide; Lactates; Methemoglobin; Rabbits; Sulfhydryl Compounds; Triazoles | 1969 |
Existence and reactivity of sulfhydryl groups in pyocin R.
Topics: Amino Acids; Bacteriocins; Benzoates; Chloromercuribenzoates; Chromatography, Paper; Cysteine; Cystine; Dinitrophenols; Electrophoresis; Ethylmaleimide; Nitrobenzenes; Pseudomonas aeruginosa; Spectrum Analysis; Sulfhydryl Compounds | 1969 |
Structural and functional roles of the cysteine residues in the alpha subunit of the Escherichia coli tryptophan synthetase. I. Structural roles and reactivity of the cysteine residues.
Topics: Animals; Antigens; Benzoates; Carbon Isotopes; Chemical Phenomena; Chemistry; Cysteine; Dextrans; Electrophoresis; Electrophoresis, Disc; Escherichia coli; Ethylmaleimide; Hydro-Lyases; Immune Sera; Immunodiffusion; Iodoacetates; Kinetics; Mercury; Methods; Neutralization Tests; Nitrobenzenes; Peptides; Rabbits; Sulfides; Tryptophan; Ultracentrifugation | 1969 |
Biochemical studies of bacterial sporulation and germination. XVII. Sulfhydryl and disulfide levels in dormancy and germination.
Topics: Bacillus megaterium; Carbon Isotopes; Cysteine; Ethylmaleimide; Spores; Sulfhydryl Compounds; Sulfides | 1969 |
The effect of sulfhydryl compounds and mercurials on the swelling rate of cells of Pseudomonas aeruginosa in sodium and potassium buffers.
Topics: Buffers; Cell Membrane Permeability; Chloromercuribenzoates; Cysteine; Depression, Chemical; Ethylmaleimide; Glycols; Hydantoins; Hydrogen Peroxide; Hydrogen-Ion Concentration; Iodine; Mercaptoethylamines; Potassium; Pseudomonas aeruginosa; Sodium; Spectrophotometry; Sulfhydryl Compounds | 1970 |
Solubilization and chemical and immunochemical characterization of sparingly soluble canine gastric mucin.
Topics: Alkylation; Amino Acids; Animals; Boron Compounds; Chemical Phenomena; Chemistry; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Thin Layer; Cysteine; Dogs; Electrophoresis; Ethylmaleimide; Fucose; Galactose; Gastric Mucins; Gastric Mucosa; Glucosamine; Glycoproteins; Hexosamines; Immunodiffusion; Immunoelectrophoresis; Neuraminic Acids; Oxidation-Reduction; Phosphates; Proline; Rabbits; Serine; Sodium; Sodium Chloride; Sodium Hydroxide; Solubility; Sulfhydryl Reagents; Sulfites; Sulfur Isotopes; Threonine | 1971 |
The reaction of 4-(p-trimethylammoniumphenylazo)-2-hydroxymercuriphenol with the thiol groups of proteins.
Topics: Animals; Azo Compounds; Cattle; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Thin Layer; Cysteamine; Cysteine; Edetic Acid; Ethylmaleimide; Glutathione; Guanidines; Hydrogen-Ion Concentration; Lactoglobulins; Mercury; Organometallic Compounds; Ovalbumin; Phenols; Protein Denaturation; Quaternary Ammonium Compounds; Serum Albumin, Bovine; Spectrophotometry; Sulfhydryl Compounds; Sulfhydryl Reagents; Thioglycolates; Urea | 1971 |
Specific modification, isolation, and partial characterization of an erythrocyte membrane protein.
Topics: Amino Acids; Blood Proteins; Carbon Isotopes; Cell Membrane; Chromatography, Gel; Chromatography, Ion Exchange; Cysteine; Electrophoresis; Erythrocytes; Ethylmaleimide; Humans; Iodoacetates; Sodium Chloride; Sodium Dodecyl Sulfate; Tritium | 1972 |
Nonspecific esterases activity and degranulation in the mast cell.
Topics: Animals; Bile Acids and Salts; Chloromercuribenzoates; Copper; Cyanides; Cysteine; Cytoplasmic Granules; Esterases; Ethylmaleimide; Fluorides; Iodoacetates; Lead; Male; Mast Cells; Mice; Mice, Inbred Strains; Physostigmine; Propionates; Skin | 1972 |
Inactivation of succinate dehydrogenase by N-ethylmaleimide. Stoichiometry and chemistry.
Topics: Alkylation; Animals; Bromine; Carbon Isotopes; Cattle; Chromatography, Gel; Cysteine; Electrophoresis, Disc; Enzyme Activation; Ethylmaleimide; Flavins; Flavoproteins; Kinetics; Malonates; Myocardium; Sodium Dodecyl Sulfate; Spectrophotometry; Succinate Dehydrogenase | 1972 |
Glutathione synthesis in human erythrocytes. II. Purification and properties of the enzymes of glutathione biosynthesis.
Topics: Acetamides; Amino Acids; Antimetabolites; Biological Assay; Carbon Isotopes; Catalysis; Chemical Phenomena; Chemistry, Physical; Chromatography; Chromatography, DEAE-Cellulose; Cysteine; Electrophoresis, Disc; Erythrocytes; Ethylmaleimide; Glutamates; Glutathione; Glycine; Humans; Ligases; Sulfhydryl Compounds; Tritium; Ultracentrifugation | 1971 |
Evidence on erythrocyte aminopeptidase B.
Topics: Aminopeptidases; Animals; Arginine; Borates; Chloromercuribenzoates; Cysteine; Dithiothreitol; Drug Stability; Electrophoresis, Disc; Enzyme Activation; Erythrocytes; Ethylmaleimide; Hot Temperature; Isoenzymes; Kinetics; Liver; Lysine; Mathematics; Methylene Blue; Naphthalenes; Phosphates; Photolysis; Radiation Effects; Rats; Sodium Chloride | 1971 |
Comparative structural studies of the active site of ATP: guanidine phosphotransferases. The essential cysteine tryptic peptide of lombricine kinase from Lumbricus terrestris muscle.
Topics: Amino Acid Sequence; Animals; Annelida; Arginine; Binding Sites; Brain; Carbon Isotopes; Cattle; Chromatography, Gel; Chromatography, Ion Exchange; Creatine Kinase; Crustacea; Cysteine; Digestion; Ethylmaleimide; Guanidines; Hydrolysis; Macromolecular Substances; Molecular Weight; Muscles; Peptides; Phosphotransferases; Rabbits; Trypsin | 1971 |
Sensitive polarographic estimation of thiol groups with N-ethylmaleimide.
Topics: Benzoates; Cysteine; Disulfides; Ethylmaleimide; Insulin; Methods; Nitro Compounds; Polarography; Spectrophotometry; Sulfhydryl Compounds | 1971 |
The basic trypsin inhibitor of bovine pancreas. 8. Changes in activity following substitution of reduced half-cystin res4ues 14 and 38 with sulfhydryl reagents.
Topics: Amines; Aprotinin; Benzoates; Boron Compounds; Chemical Phenomena; Chemistry; Chymotrypsin; Cysteine; Ethylmaleimide; Iodoacetates; Mercury; Nitrobenzenes; Sulfonic Acids; Trypsin Inhibitors | 1968 |
Sulphur metabolism: the usefulness of N-ethylmaleimide.
Topics: Chromatography; Cysteine; Escherichia coli; Ethylmaleimide; Glutathione; Methionine; Sulfur; Sulfur Isotopes | 1966 |
[Kinetics of the reaction of imidazole-SH-compounds with N-ethylmaleimide].
Topics: Chemical Phenomena; Chemistry; Cysteine; Ethylmaleimide; Glutathione; Hydrogen-Ion Concentration; Imidazoles; Kinetics; Sulfhydryl Compounds | 1969 |
Studies on the mechanism of fatty acid synthesis. 23. The acyl binding sites of the pigeon liver fatty acid synthetase.
Topics: Acetates; Amides; Amino Acids; Animals; Binding Sites; Caproates; Carbon Isotopes; Chromatography, Ion Exchange; Coenzyme A; Columbidae; Cysteine; Ethylmaleimide; Fatty Acids; Ligases; Liver; Malonates; Oxidation-Reduction; Serine; Sulfhydryl Compounds | 1970 |
The development of Haemonchus contortus in vitro. II. The effect of disulphide-reducing and sulphydryl-blocking reagents on the rate of development to the fourth-stage larvae.
Topics: Animals; Benzoates; Carbon Dioxide; Cysteine; Depression, Chemical; Ethylmaleimide; Glutathione; Iodoacetates; Stimulation, Chemical; Time Factors; Trichostrongyloidea | 1970 |
Studies on lysine-2,3-aminomutase. Subunit structure and sulfhydryl groups.
Topics: Amino Acids; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Chromatography, Gel; Clostridium; Cysteine; Detergents; Dialysis; Ethylmaleimide; Glutathione; Hydrogen-Ion Concentration; Iodoacetates; Isomerases; Lysine; Methionine; Molecular Weight; Protein Denaturation; Sulfhydryl Compounds; Sulfuric Acids; Urea | 1970 |
[Kinetics of the reaction of papain with N-ethylmaleimide].
Topics: Cysteine; Drug Antagonism; Ethylmaleimide; Hydrogen-Ion Concentration; Papain; Spectrophotometry; Sulfhydryl Compounds | 1970 |
Further studies on the isozymic forms of soluble rat liver guanine deaminase.
Topics: Aminohydrolases; Animals; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Guanidines; Guanine; Isoenzymes; Liver; Mercaptoethanol; Oxidation-Reduction; Protein Denaturation; Rats; Sulfhydryl Compounds; Urea | 1970 |
The essential thiol group of arginine kinase from Homarus vulgaris muscle studied by difference spectrophotometry and N-ethyl-[1-14C] maleimide labelling.
Topics: Amino Acid Sequence; Animals; Arginine; Brain; Carbon Isotopes; Chemical Phenomena; Chemistry; Creatine Kinase; Crustacea; Cysteine; Ethylmaleimide; Glutathione; Models, Chemical; Muscles; Peptides; Phosphotransferases; Rabbits; Spectrophotometry; Sulfhydryl Compounds; Trypsin; Tyrosine | 1970 |
Effect of thiols in biological systems on protein sulfhydryl content.
Topics: Acetamides; Animals; Autoradiography; Blood Proteins; Cysteine; Erythrocytes; Ethylmaleimide; Glycine; Humans; Kidney; Liver; Lung; Mice; Muscles; Penicillamine; Polarography; Skin; Spleen; Sulfhydryl Compounds; Sulfhydryl Reagents; Sulfur Isotopes; Tetroses; Time Factors | 1970 |
Oxidizing-reducing properties of the intrinsic prothrombin activator.
Topics: Absorption; Aluminum Silicates; Barbiturates; Barium Sulfate; Biochemical Phenomena; Biochemistry; Blood Coagulation Tests; Cysteine; Enzyme Activation; Ethylmaleimide; Glutathione; Hemophilia A; Humans; Nitrogen; Oxidation-Reduction; Oxygen; Prothrombin; Thrombin | 1970 |
Mg 2+ -activated ATP hydrolysis and sulfhydryl groups in membranes from human erythrocytes.
Topics: Adenosine Triphosphate; Amino Acids; Benzoates; Blood Proteins; Cell Membrane; Chloromercuribenzoates; Cysteine; Disulfides; Drug Synergism; Erythrocytes; Ethylmaleimide; Humans; Hydrogen-Ion Concentration; Hydrolysis; Magnesium; Nitro Compounds; Potassium; Sulfhydryl Compounds; Ultracentrifugation | 1970 |
Development of fluorescence between o-phthaldialdehyde and thiols.
Topics: Alcohol Oxidoreductases; Aldehydes; Animals; Cattle; Cysteine; Ethylmaleimide; Fluorescence; Glutathione; Hemoglobins; Homocysteine; Insulin; Liver; Mercaptoethanol; Phthalic Acids; Serum Albumin, Bovine; Sulfhydryl Compounds | 1970 |
Purification and characterization of L-serine transacetylase and O-acetyl-L-serine sulfhydrylase from kidney bean seedlings (Phaseolus vulgaris).
Topics: Acetates; Acyltransferases; Carbon Isotopes; Chemical Precipitation; Chloromercuribenzoates; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Lyases; Plants; Pyridoxal Phosphate; Quaternary Ammonium Compounds; Serine; Sulfates; Sulfhydryl Reagents | 1971 |
Some properties of an SH group essential for choline transport in human erythrocytes.
Topics: Biological Transport, Active; Bis-Trimethylammonium Compounds; Choline; Cystamine; Cysteine; Depression, Chemical; Erythritol; Erythrocytes; Ethylmaleimide; Glutathione; Humans; In Vitro Techniques; Mercaptoethanol; Mercury; Quaternary Ammonium Compounds; Sulfonic Acids | 1971 |
Transsulfuration in higher plants. Partial purification and properties of beta-cystathionase of spinach.
Topics: Alanine; Amino Acids; Benzoates; Carbon Isotopes; Chemical Phenomena; Chemistry; Chromatography, Paper; Cysteine; Drug Stability; Edible Grain; Ethylmaleimide; Homocysteine; Hydro-Lyases; Hydrogen-Ion Concentration; Hydroxylamines; Kinetics; Models, Structural; Plants; Pyridoxal Phosphate; Pyruvates; Quaternary Ammonium Compounds; Serine; Species Specificity; Spectrophotometry; Stereoisomerism; Sulfates; Sulfides; Sulfur; Triticum | 1971 |
Osmotic regulation in a halophilic strain of Chlamydomonas. II. Effects of sulfhydryl reagents.
Topics: Chloromercuribenzoates; Cysteine; Ethylmaleimide; Eukaryota; Hypotonic Solutions; Osmosis; Sulfhydryl Compounds; Water | 1967 |
The interactions between alpha and beta chains of human hemoglobin.
Topics: Amides; Benzoates; Carbon Monoxide; Carboxypeptidases; Chemical Phenomena; Chemistry; Cysteine; Ethylmaleimide; Fluorine; Hemoglobins; Humans; Iodoacetates; Kinetics; Nitrobenzenes; Oxygen; Sulfhydryl Compounds | 1968 |
The essential thiol group of sheep kidney mitochondrial phosphoenolpyruvate carboxykinase.
Topics: Animals; Benzoates; Carbon Isotopes; Carboxy-Lyases; Catalysis; Cysteine; Electrophoresis; Ethylmaleimide; Hydrogen-Ion Concentration; Iodoacetates; Kidney; Kinetics; Methylation; Mitochondria; Nitrobenzenes; Nucleotides; Phosphates; Pyruvates; Sheep | 1968 |
[The S04= transport system of Chlorella pyrenoidosa and its regulation. Research on the regulation of entry].
Topics: Absorption; Biological Transport; Chloromercuribenzoates; Chromates; Cysteine; Depression, Chemical; Ethylmaleimide; Eukaryota; Methionine; Oxidation-Reduction; Phenylalanine; Sulfates; Sulfhydryl Compounds; Sulfonic Acids; Sulfur; Time Factors | 1968 |
Indoleacetaldoxime hydro-lyase. II. Purification and properties.
Topics: Ascomycota; Ascorbic Acid; Benzoates; Chelating Agents; Cyanides; Cysteine; Dimercaprol; Ethylmaleimide; Glutathione; Hydro-Lyases; Hydrogen-Ion Concentration; Kinetics; Mercaptoethanol; Mercury; Oxidation-Reduction; Pyridoxal Phosphate; Thermodynamics | 1968 |
The kinetics of the reaction of n-ethylmaleimide with denatured beta-lactoglobulin and ovalbumin.
Topics: Chemical Phenomena; Chemistry; Chlorides; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Lactoglobulins; Mercury; Ovalbumin; Spectrophotometry; Sulfhydryl Compounds; Surface-Active Agents; Urea | 1968 |
Effect of certain chemical compounds on rat liver aminopeptidase B acting on L-arginyl-2-naphthylamide with evidence of the occurrence of a similar enzyme in the liver of human fetuses.
Topics: Amides; Aminopeptidases; Animals; Arginine; Carbamates; Cysteine; Ethylmaleimide; Fetus; Humans; Ketones; Kinetics; Liver; Naphthalenes; Rats; Succinates | 1968 |
Effects of various compounds on growth of yeast cells of Histoplasma capsulatum.
Topics: Antifungal Agents; Benzoates; Cephalothin; Cysteine; Cystine; Ethylmaleimide; Histoplasma; Imides; Indoleacetic Acids; Indoles; Melatonin; Penicillins; Sulfides; Sulfur; Thiamine; Thiocarbamates | 1968 |
Acylation reactions with cyclic imides.
Topics: Amino Acids; Chemical Phenomena; Chemistry; Chromatography, Paper; Cysteine; Drug Stability; Ethylmaleimide; Glutathione; Heterocyclic Compounds; Homocystine; Hydrogen-Ion Concentration; Imides; Sulfhydryl Compounds | 1968 |
Subunit structure and isolation of a peptide containing the reactive cysteine group of beta-methylaspartase.
Topics: Amino Acids; Aspartic Acid; Binding Sites; Carbon Isotopes; Chromatography; Chromatography, Gel; Chromatography, Ion Exchange; Cysteine; Electrophoresis; Ethylmaleimide; Fumarates; Lyases; Peptides; Protein Hydrolysates; Sulfhydryl Compounds; Trypsin | 1968 |
Immune reactions of human blood platelets. 3. Inhibition of immune reactions by sulfhydryl reagents.
Topics: Antigen-Antibody Reactions; Blood Platelets; Cysteine; Ethylmaleimide; Hirudins; Humans; Immune Sera; Iodoacetates; Spectrophotometry; Sulfhydryl Compounds; Thrombin | 1968 |
Beta-fructofuranosidase activity in pea seeds.
Topics: Amides; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Fructose; Glutathione; Glycoside Hydrolases; Hydrogen-Ion Concentration; Mercaptoethanol; Plants, Edible; Seeds | 1968 |
The enzymic synthesis of cysteine from inorganic sulphide and L-serine by rat liver preparations.
Topics: Animals; Culture Techniques; Cysteine; Enzyme Induction; Enzyme Precursors; Ethylmaleimide; Hydro-Lyases; Hydrogen-Ion Concentration; Infrared Rays; Kinetics; Liver; Male; Rats; Serine; Spectrum Analysis; Sulfides; Sulfur Isotopes | 1968 |
[Action of dithiothreitol in irradiated rats after treatment with NEM].
Topics: Alcohols; Animals; Cysteine; Ethylmaleimide; Radiation Effects; Radiation-Protective Agents; Radiation-Sensitizing Agents; Rats; Sulfur | 1968 |
[Action of some chemical reagents on the active protein groups of the hemagglutinin of rubella virus].
Topics: Anhydrides; Aniline Compounds; Antigens; Benzoates; Copper; Cysteine; Ethylmaleimide; Hemagglutination, Viral; Hemagglutinins, Viral; Iodoacetates; Mercaptoethanol; Mercury; Rubella virus; Sulfates; Sulfonic Acids; Viral Proteins | 1968 |
Thin-layer chromatographic separation of cystine and the N-ethylmaleimide adducts of cysteine and glutathionen.
Topics: Amino Acids; Carbon Isotopes; Chemical Phenomena; Chemistry; Chromatography, Thin Layer; Cysteine; Cystine; Ethylmaleimide; Glutathione; Sulfur Isotopes | 1969 |
Effect of sulfhydryl groups on 5-aminolaevulinate dehydratase activity.
Topics: Animals; Copper; Cysteine; Depression, Chemical; Ethylmaleimide; Hydro-Lyases; Liver; Rabbits; Saccharomyces | 1969 |
Isolation and characterization of lipid N-methylrtansferase from dog lung.
Topics: Adenine Nucleotides; Animals; Azides; Carbon Dioxide; Centrifugation, Density Gradient; Chloromercuribenzoates; Chromatography, Ion Exchange; Cyanides; Cysteine; Dogs; Ethylmaleimide; Hydrogen-Ion Concentration; Liver; Lung; Magnesium; Maleates; Mercury; Methionine; Methylation; Microscopy, Electron; Microsomes; Oxygen; Phosphatidylcholines; Phosphatidylethanolamines; Temperature; Transferases | 1969 |
Preparation and studies on the characterization of sheep brain glutamine synthetase.
Topics: Adenosine Triphosphate; Amino Acids; Animals; Arginine; Benzoates; Brain; Bromine; Cellulose; Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Cyanides; Cysteine; Drug Stability; Ethylmaleimide; Glutamine; Hydroxyapatites; Ligases; Magnesium; Methods; Peptides; Sheep; Sulfhydryl Compounds; Sulfides; Trypsin | 1969 |
Purification and general properties of argininosuccinate lyase from jack bean, Canavalia ensiformis (L.)DC.
Topics: Arginine; Benzoates; Chemical Phenomena; Chemistry; Chromatography, Gel; Cysteine; Detergents; Drug Stability; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Lipids; Lyases; Mercury; Plants; Succinates; Sulfates; Temperature; Time Factors | 1969 |
Characterization of sulfhydryl groups of actin.
Topics: Amino Acids; Animals; Azo Compounds; Benzoates; Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Chromatography, Paper; Coloring Agents; Cysteine; Electrophoresis, Disc; Ethylmaleimide; Glutathione; Mercury; Muscle Proteins; Nitrates; Organometallic Compounds; Peptides; Protein Binding; Protein Denaturation; Rabbits; Radioisotopes; Silver; Sulfhydryl Compounds; Ultracentrifugation; Viscosity | 1969 |
The effect of metabolic inhibitors on transferrin and iron uptake and transferrin release from reticulocytes.
Topics: Animals; Arsenic; Azides; Biological Transport; Bloodletting; Chloromercuribenzoates; Cyanides; Cycloheximide; Cysteine; Depression, Chemical; Dinitrophenols; Electron Transport; Ethylmaleimide; Female; Fluorides; In Vitro Techniques; Iodine Isotopes; Iron; Iron Isotopes; Lead; Male; Malonates; Oligomycins; Ouabain; Rabbits; Reticulocytes; Rotenone; Transferrin | 1969 |
Effects of a sulfhydry reagent, N-ethylmaleimide, on electrical and mechanical activities of isolated atria.
Topics: Acetylcholine; Adenosine Triphosphate; Animals; Cysteine; Electric Stimulation; Ethylmaleimide; Female; Heart; Heart Atria; Heart Rate; In Vitro Techniques; Male; Membrane Potentials; Muscle Contraction; Muscle, Smooth; Norepinephrine; Rabbits; Sinoatrial Node | 1969 |
Reactions of N-ethylmaleimide with peptides and amino acids.
Topics: Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Cysteine; Dipeptides; Ethylmaleimide; Hemoglobins; Homocysteine; Ribonucleases | 1964 |
The role of -SH groups in the enzymic activity of phosphorylase-b.
Topics: Adenine Nucleotides; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Glucosyltransferases; Glycogen; Hexosephosphates; Kinetics; Mercaptoethanol; Sulfhydryl Compounds | 1965 |
Inhibition of carbohydrate metabolism by oxygen and N-ethylmaleimide in rat heart homogenates.
Topics: Adenosine Triphosphate; Animals; Cysteine; Ethylmaleimide; Glutathione; Glycols; Glycolysis; In Vitro Techniques; Myocardium; Oxygen; Rats; Sulfhydryl Compounds | 1966 |
Cystathionine gamma-synthetase of Salmonella. Catalytic properties of a new enzyme in bacterial methionine biosynthesis.
Topics: Amino Acids; Catalysis; Cysteine; Ethylmaleimide; Kinetics; Ligases; Methionine; Molecular Biology; Mutation; Propionates; Salmonella; Ultracentrifugation | 1966 |
Mechanism of action of guinea pig liver transglutaminase. I. Purification and properties of the enzyme: identification of a functional cysteine essential for activity.
Topics: Amides; Amino Acids; Animals; Benzoates; Catalysis; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Chromatography; Cysteine; Ethylmaleimide; Glutaminase; Guinea Pigs; In Vitro Techniques; Liver; Molecular Weight; Spectrophotometry; Ultracentrifugation | 1966 |
The inorganic sulfate transport system of Penicillium chrysogenum.
Topics: Amides; Biological Transport, Active; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Methionine; Penicillium; Sulfates; Thiosulfates | 1966 |
Kinetics of the reaction of N-ethylmaleimide with cysteine and some congeners.
Topics: Chemical Phenomena; Chemistry; Cysteine; Ethylmaleimide; Glutathione; Hydrogen-Ion Concentration; Kinetics; Mercaptoethylamines; Propylene Glycols; Sulfhydryl Compounds | 1966 |
Studies on the denaturation of biological macromolecules by chemical carcinogens.
Topics: Benzoates; Carcinogens; Chemical Phenomena; Chemistry; Cysteine; Ethylmaleimide; Ferrocyanides; Glutathione; Hydrogen Peroxide; Iodides; Iodine; Mercury; Nitrosamines; Ovalbumin; Spectrum Analysis; Sulfhydryl Compounds | 1966 |
Inhibition of dopamine beta-hydroxylase by sulfhydryl compounds and the nature of the natural inhibitors.
Topics: Adrenal Medulla; Animals; Cattle; Chromaffin System; Coenzyme A; Copper; Cysteine; Cystine; Detergents; Dopamine; Enzymes; Ethylmaleimide; Glutathione; Mercaptoethanol; Mixed Function Oxygenases; Rats; Sulfhydryl Compounds | 1967 |
Increased cystine in leukocytes from individuals homozygous and heterozygous for cystinosis.
Topics: Acid Phosphatase; Cell Nucleus; Cysteine; Cystine; Cystinosis; Cytoplasmic Granules; Ethylmaleimide; Humans; Leukocytes; Molecular Biology | 1967 |
The essential thiol group of propionyl-CoA carboxylase.
Topics: Adenosine Triphosphate; Animals; Chemical Phenomena; Chemistry; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Ligases; Myocardium; Sulfhydryl Compounds; Swine | 1967 |
Studies on cat hemoglobin and hybrids with human hemoglobin A.
Topics: Animals; Benzoates; Cats; Cysteine; Dogs; Electrophoresis; Ethylmaleimide; Gels; Hemoglobins; Humans; Hybridization, Genetic; Mercury; Oxygen; Peptides; Spectrophotometry; Starch; Sulfhydryl Compounds | 1967 |
Effect of sulfhydryl modification on the activities of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Topics: Acetamides; Amino Acids; Animals; Carbon Radioisotopes; Cysteine; Ethylmaleimide; Kinetics; Liver; Mercuribenzoates; Phosphofructokinase-2; Phosphoric Monoester Hydrolases; Phosphotransferases; Protein Binding; Rats | 1984 |
Localization of the N-ethylmaleimide reactive cysteine in the beef heart mitochondrial ADP/ATP carrier protein.
Topics: Alkylation; Amino Acid Sequence; Amino Acids; Animals; Cattle; Cysteine; Ethylmaleimide; Methionine; Mitochondria, Heart; Mitochondrial ADP, ATP Translocases; Nucleotidyltransferases; Peptide Fragments | 1984 |
The NH2 terminus of the (Ca2+ + Mg2+)-adenosine triphosphatase is located on the cytoplasmic surface of the sarcoplasmic reticulum membrane.
Topics: Amino Acid Sequence; Animals; Ca(2+) Mg(2+)-ATPase; Calcium-Transporting ATPases; Chemical Phenomena; Chemistry; Cysteine; Ethylmaleimide; Intracellular Membranes; Peptide Fragments; Sarcoplasmic Reticulum; Sulfhydryl Reagents | 1981 |
[Semiconservative and unscheduled DNA-synthesis of rat thymocytes under the influence of some radioprotecting and radiosensitizing agents (author's transl)].
Topics: Acetophenones; Animals; beta-Aminoethyl Isothiourea; Bleomycin; Cysteamine; Cysteine; Cytarabine; Ditiocarb; DNA; Doxorubicin; Ethidium; Ethylmaleimide; Female; Fluorouracil; Glutathione; Mercaptoethanol; Metronidazole; Nitrofurazone; Radiation-Protective Agents; Radiation-Sensitizing Agents; Rats; T-Lymphocytes; Tiopronin | 1982 |
Studies on the mechanism of the stimulation of polymerase II-catalyzed RNA synthesis by mercury compounds.
Topics: Amanitins; Cetrimonium; Cetrimonium Compounds; Cysteine; Deoxycholic Acid; DNA-Directed RNA Polymerases; Ethylmaleimide; Heparin; Humans; Methylmercury Compounds; Octoxynol; Polyethylene Glycols; RNA; RNA Polymerase II; Transcription, Genetic | 1983 |
[Comparative studies on the DNA synthesis of rat thymus and spleen cells in vitro under the influence of x rays, UV light and radiomimetic compounds].
Topics: Animals; Bleomycin; Cysteine; Cytarabine; DNA; DNA Repair; Ethylmaleimide; Female; Novobiocin; Rats; Spleen; Thymidine; Thymus Gland; Tritium; Ultraviolet Rays; X-Rays | 1984 |
Sulfhydryl groups of the F1 adenosine triphosphatase of Escherichia coli and the stoichiometry of the subunits.
Topics: Affinity Labels; Cysteine; Escherichia coli; Ethylmaleimide; Iodoacetamide; Isoelectric Focusing; Macromolecular Substances; Proton-Translocating ATPases; Sulfhydryl Compounds | 1984 |
Characterization of the cysteinyl-containing peptides of the gamma subunit of coupling factor 1.
Topics: Amino Acids; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Ethylmaleimide; Macromolecular Substances; Peptide Fragments; Plants; Proton-Translocating ATPases; Pyridines; Trypsin | 1984 |
Activation of human leukocyte collagenase by compounds reacting with sulfhydryl groups.
Topics: Chromatography, Gel; Cysteine; Enzyme Activation; Ethylmaleimide; Hot Temperature; Humans; Kinetics; Microbial Collagenase; Neutrophils; Phenylmercuric Acetate; Sulfhydryl Reagents | 1980 |
Electron spin resonance studies of bovine plasma amine oxidase. Probing of the environment about the substrate-liberated sulfhydryl groups in the active site.
Topics: Amine Oxidase (Copper-Containing); Animals; Binding Sites; Cattle; Chemical Phenomena; Chemistry; Circular Dichroism; Copper; Cysteine; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Hydrogen-Ion Concentration; Protein Conformation; Spin Labels | 1980 |
Modification of ultraviolet radiation effects on the membrane of myelinated nerve fibers by sulfhydryl compounds.
Topics: Animals; Cysteine; Ethylmaleimide; In Vitro Techniques; Ion Channels; Membrane Proteins; Mercaptoethanol; Nerve Tissue Proteins; Rana esculenta; Ranvier's Nodes; Sciatic Nerve; Sodium; Sulfhydryl Compounds; Ultraviolet Rays | 1984 |
Chemical modification of sulfhydryl groups in p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Involvement in catalysis and assignment in the sequence.
Topics: 4-Hydroxybenzoate-3-Monooxygenase; Amino Acid Sequence; Binding Sites; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Kinetics; Mixed Function Oxygenases; p-Chloromercuribenzoic Acid; Pseudomonas fluorescens; Sulfhydryl Reagents | 1984 |
Partial amino-acid sequence and cysteine reactivities of cytosolic aspartate aminotransferase from horse heart.
Topics: Amino Acid Sequence; Animals; Aspartate Aminotransferases; Chymotrypsin; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Horses; Isoenzymes; Myocardium; Peptide Fragments; Swine | 1984 |
Ligand-induced variations in the reactivity of thio groups of the alpha-subunit of the acetylcholine receptor from Torpedo californica.
Topics: Acetylcholine; Animals; Binding, Competitive; Cysteine; Electric Organ; Ethylmaleimide; Kinetics; Ligands; Macromolecular Substances; Molecular Weight; Receptors, Cholinergic; Torpedo | 1984 |
Identification of active-site residues of sheep liver serine hydroxymethyltransferase.
Topics: Animals; Arginine; Binding Sites; Cysteine; Diethyl Pyrocarbonate; Ethylmaleimide; Glycine Hydroxymethyltransferase; Histidine; Kinetics; Liver; Phenylglyoxal; Sheep; Spectrophotometry; Transferases | 1984 |
Transport of glutathione by intestinal brush border membrane vesicles.
Topics: Animals; Biological Transport; Cysteine; Dipeptides; Ethylmaleimide; Glutathione; Intestinal Absorption; Intestinal Mucosa; Jejunum; Kinetics; Microvilli; Oligopeptides; Osmolar Concentration; Swine | 1984 |
Inhibition of polyoma virus middle T antigen-associated tyrosyl kinase activity by N-ethylmaleimide.
Topics: Adenine Nucleotides; Animals; Antigens, Polyomavirus Transforming; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Protein Kinase Inhibitors; Protein-Tyrosine Kinases; Viral Proteins | 1983 |
Chemical modification of Cys-374 of actin interferes with the formation of the profilactin complex.
Topics: Actins; Animals; Cattle; Contractile Proteins; Cysteine; Ethylmaleimide; Fluorescent Dyes; Iodoacetamide; Iodoacetates; Macromolecular Substances; Microfilament Proteins; Profilins; Proteins; Spectrometry, Fluorescence; Spleen | 1984 |
The reactivity of sulfhydryl groups of yeast DNA dependent RNA polymerase I.
Topics: Chloromercuribenzoates; Cysteine; Dithionitrobenzoic Acid; DNA-Directed RNA Polymerases; Ethylmaleimide; Kinetics; Mercaptoethanol; p-Chloromercuribenzoic Acid; Protein Binding; RNA Polymerase I; Saccharomyces cerevisiae | 1982 |
Effects of thiol reagents on glucose transport in thymocytes.
Topics: Amino Acids; Animals; Biological Transport, Active; Calcimycin; Calcium; Cysteine; Dithiothreitol; Edetic Acid; Ethylmaleimide; Glutathione; In Vitro Techniques; Kinetics; Methylglucosides; Methylglycosides; Rats; Sulfhydryl Compounds; Thymus Gland | 1981 |
Alterations in pharmacological receptor activities of rabbit arteries by sulfhydryl reagents.
Topics: Animals; Calcium; Cysteine; Dithiothreitol; Ethylmaleimide; Female; In Vitro Techniques; Male; Mercaptoethanol; Mesenteric Arteries; Muscle Contraction; Muscle, Smooth, Vascular; Rabbits; Receptors, Drug; Sulfhydryl Reagents | 1983 |
Sulfhydryl groups of Escherichia coli ribosomal protein S1. Location along the polypeptide chain.
Topics: Cysteine; Escherichia coli; Ethylmaleimide; Peptides; Ribosomal Proteins; Thiocyanates; Urea | 1980 |
Location of the reactive sulfhydryl residues in the primary sequence of the beta 2 subunit of tryptophan synthase of Escherichia coli.
Topics: Affinity Labels; Amino Acid Sequence; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Macromolecular Substances; Peptide Fragments; Pyridoxal Phosphate; Tryptophan Synthase | 1980 |
Identification of an active site cysteine residue in Escherichia coli pyruvate oxidase.
Topics: Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Kinetics; Protein Binding; Pyruvate Oxidase | 1982 |
Defective cystine exodus from isolated lysosome-rich fractions of cystinotic leucocytes.
Topics: Cysteine; Cystine; Cystinosis; Ethylmaleimide; Half-Life; Heterozygote; Humans; Leukocytes; Lysosomes | 1982 |
Cystine transport is defective in isolated leukocyte lysosomes from patients with cystinosis.
Topics: Biological Transport; Carrier Proteins; Cysteine; Cystine; Cystinosis; Ethylmaleimide; Humans; Kinetics; Leukocytes; Lysosomes | 1982 |
Effect of hydroxynitrobenzylation of tryptophan-177 on reactivity of active site cysteine-25 in papain.
Topics: Acetamides; Acetates; Alkylating Agents; Binding Sites; Cysteine; Ethylmaleimide; Glycine; Kinetics; Nitrophenols; Papain; Tryptophan | 1981 |
The N-ethylmaleimide-sensitive cysteine residue in the pH-dependent subunit interactions of malate dehydrogenase.
Topics: Animals; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Macromolecular Substances; Malate Dehydrogenase; Mitochondria, Heart; Molecular Weight; NAD; Oxidation-Reduction; Swine | 1981 |
Use of N-ethylmaleimide to prevent interference by sulfhydryl reagents with the glucose oxidase assay for glucose.
Topics: Cysteine; Ethylmaleimide; False Negative Reactions; Glucose; Glucose Oxidase; Glutathione; Methods; Oxidation-Reduction; Peroxidases; Sulfhydryl Reagents | 1981 |
Transport interaction of L-cystine and L-glutamate in human diploid fibroblasts in culture.
Topics: Amino Acids; Biological Transport; Cell Line; Cysteine; Cystine; Diploidy; Ethylmaleimide; Glutamates; Humans; Kinetics; Lung | 1980 |
A diagonal procedure for isolating sulfhydryl peptides alkylated with N-ethylmaleimide.
Topics: Alkylation; Cysteine; Electrophoresis, Paper; Ethylmaleimide; Peptides | 1980 |
Effects of cysteine modification on the activity of the cGMP-gated channel from retinal rods.
Topics: Animals; Blotting, Western; Calcium; Calcium Channels; Cattle; Cyclic GMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Diamide; Dithiothreitol; Ethylmaleimide; Glutathione; Glutathione Disulfide; Ion Channel Gating; Ion Channels; Lipid Bilayers; Mersalyl; Nitric Oxide; Rod Cell Outer Segment; Sodium; Sulfhydryl Reagents | 1995 |
Mutational analysis of the [4Fe-4S]-cluster converting iron regulatory factor from its RNA-binding form to cytoplasmic aconitase.
Topics: Aconitate Hydratase; Animals; Cysteine; Cytoplasm; Diamide; Enzyme Activation; Ethylmaleimide; Humans; Iron; Iron-Regulatory Proteins; L Cells; Mercaptoethanol; Mice; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Binding; Protein Conformation; RNA; RNA-Binding Proteins; Substrate Specificity; Transfection | 1994 |
NMDA receptor channels: subunit-specific potentiation by reducing agents.
Topics: Alanine; Animals; Base Sequence; Cell Line; Cloning, Molecular; Cysteine; Dithiothreitol; Drug Synergism; Ethylmaleimide; Glutamates; Glutamic Acid; Glutathione; Humans; Ion Channels; Kidney; Kinetics; Macromolecular Substances; Membrane Potentials; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Rats; Receptors, N-Methyl-D-Aspartate; Recombinant Fusion Proteins; Serine; Transfection | 1994 |
Stabilities of the fluorescent SH-reagent eosin-5-maleimide and its adducts with sulfhydryl compounds.
Topics: Acetylcysteine; Chromatography, High Pressure Liquid; Cysteine; Drug Stability; Eosine Yellowish-(YS); Ethylmaleimide; Glutathione; Hydrogen-Ion Concentration; Kinetics; Mass Spectrometry; Spectrometry, Fluorescence; Spectrometry, Mass, Fast Atom Bombardment; Succinimides; Sulfhydryl Compounds | 1995 |
Covalent modification of human P-glycoprotein mutants containing a single cysteine in either nucleotide-binding fold abolishes drug-stimulated ATPase activity.
Topics: Adenosine Triphosphatases; Amino Acid Sequence; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Calcium Channel Blockers; Cell Line; Cell Membrane; Consensus Sequence; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Humans; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleotides; Protein Folding; Recombinant Proteins; Sequence Deletion; Spodoptera; Transfection; Verapamil | 1995 |
Dynamics of lactose permease of Escherichia coli determined by site-directed chemical labeling and fluorescence spectroscopy.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Carbohydrate Sequence; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Fluorescent Dyes; Kinetics; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protein Structure, Secondary; Pyrenes; Recombinant Proteins; Spectrometry, Fluorescence; Sulfhydryl Reagents; Symporters; Thiogalactosides | 1995 |
Mapping of the amino acids in the cytoplasmic loop connecting helices C and D in rhodopsin. Chemical reactivity in the dark state following single cysteine replacements.
Topics: Amino Acid Sequence; Animals; Cattle; Cysteine; Cytoplasm; Darkness; Ethylmaleimide; GTP-Binding Proteins; Molecular Sequence Data; Mutation; Protein Structure, Secondary; Rhodopsin | 1995 |
ATP-dependent inactivation of the beta-Ser339Cys mutant F1-ATPase from Escherichia coli by N-ethylmaleimide.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Base Sequence; Cysteine; Edetic Acid; Escherichia coli; Ethylmaleimide; Hydrogen-Ion Concentration; Molecular Sequence Data; Mutagenesis, Site-Directed; Proton-Translocating ATPases; Serine; Structure-Activity Relationship | 1995 |
Red cell surface cysteine residue (285) of D polypeptide is not essential for D antigenicity.
Topics: Antibodies, Monoclonal; Antigen-Antibody Reactions; Cysteine; Ethylmaleimide; Humans; In Vitro Techniques; Palmitates; Rh-Hr Blood-Group System; Structure-Activity Relationship; Sulfhydryl Reagents | 1995 |
Reactivity of the H(+)-ATPase from Kluyveromyces lactis to sulfhydryl reagents.
Topics: Adenosine Diphosphate; Binding Sites; Cell Membrane; Cysteine; Ethylmaleimide; Kinetics; Kluyveromyces; Magnesium Sulfate; Methyl Methanesulfonate; Proton-Translocating ATPases | 1995 |
The role of cysteine in the alteration of bovine liver dihydrodiol dehydrogenase 3 activity.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteine; Ethylmaleimide; Isoenzymes; Liver; Molecular Sequence Data; Oxidoreductases; Sequence Homology, Amino Acid | 1995 |
Selective disulphide linkage of plant thionins with other proteins.
Topics: Antimicrobial Cationic Peptides; Bacterial Proteins; Cysteine; Cytochrome c Group; Disulfides; Dithiothreitol; Ethylmaleimide; Glucuronidase; Glutathione; Glutathione Disulfide; Kanamycin Kinase; Ovalbumin; Peptides; Phosphotransferases (Alcohol Group Acceptor); Plant Proteins; Proteins; Pseudomonas; Serum Albumin, Bovine; Toxins, Biological | 1995 |
Evidence for conformational changes in Escherichia coli porphobilinogen deaminase during stepwise pyrrole chain elongation monitored by increased reactivity of cysteine-134 to alkylation by N-ethylmaleimide.
Topics: Alkylation; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Hydroxymethylbilane Synthase; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Protein Conformation | 1995 |
Chemical modification of alpha(1-3) fucosyltransferase defines residues of catalytic importance.
Topics: Animals; Arginine; Binding Sites; Carbohydrate Sequence; Cell Line; Cloning, Molecular; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Fucosyltransferases; Histidine; Humans; Kinetics; Lysine; Molecular Sequence Data; Recombinant Proteins; Spodoptera; Transfection; Tryptophan | 1995 |
Characterization of cysteine residues of mitochondrial ADP/ATP carrier with the SH-reagents eosin 5-maleimide and N-ethylmaleimide.
Topics: Animals; Cattle; Cysteine; Eosine Yellowish-(YS); Ethylmaleimide; Kinetics; Mitochondria, Heart; Mitochondrial ADP, ATP Translocases; Sulfhydryl Reagents | 1993 |
Substrate-induced acceleration of N-ethylmaleimide reaction with the Cys-65 mutant of the transposon Tn10-encoded metal-tetracycline/H+ antiporter depends on the interaction of Asp-66 with the substrate.
Topics: Amino Acid Sequence; Antiporters; Aspartic Acid; Bacterial Proteins; Base Sequence; Cell Membrane; Cysteine; DNA Transposable Elements; Escherichia coli; Ethylmaleimide; Magnesium; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Protein Conformation; Tetracycline | 1995 |
Mechanistic studies on CDP-6-deoxy-delta 3,4-glucoseen reductase: the role of cysteine residues in catalysis as probed by chemical modification and site-directed mutagenesis.
Topics: Amino Acid Sequence; Base Sequence; Binding Sites; Catalysis; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Escherichia coli; Ethylmaleimide; Gene Expression; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Oxidoreductases; Recombinant Proteins; Structure-Activity Relationship; Trypsin; Yersinia pseudotuberculosis | 1995 |
In vivo sulfhydryl modification of the ligand-binding site of Tsr, the Escherichia coli serine chemoreceptor.
Topics: Bacterial Proteins; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Ligands; Membrane Proteins; Mutagenesis, Site-Directed; Serine; Threonine | 1995 |
Ligand-induced conformational changes in the lactose permease of Escherichia coli: evidence for two binding sites.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Bacterial Proteins; Binding Sites; Coumarins; Cysteine; Electron Spin Resonance Spectroscopy; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Isopropyl Thiogalactoside; Lactose; Ligands; Membrane Transport Modulators; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Spin Labels; Symporters; Thiogalactosides; Valine | 1994 |
The role of transmembrane domain III in the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Bacterial Proteins; Base Sequence; Biological Transport, Active; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Lactose; Membrane Transport Modulators; Membrane Transport Proteins; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Recombinant Fusion Proteins; Structure-Activity Relationship; Symporters; Thiogalactosides | 1994 |
Prohormone processing in permeabilized cells: endoproteolytic cleavage of prosomatostatin in the trans-Golgi network.
Topics: Adenosine Triphosphate; Animals; Carbonyl Cyanide m-Chlorophenyl Hydrazone; Cell Line; Cell Membrane Permeability; Chloroquine; Cysteine; Cytoplasmic Granules; Endopeptidases; Ethylmaleimide; Golgi Apparatus; Kinetics; Protein Precursors; Protein Processing, Post-Translational; Proton-Translocating ATPases; Rats; Somatostatin; Tumor Cells, Cultured | 1994 |
Site-directed mutagenesis of vacuolar H(+)-pyrophosphatase. Necessity of Cys634 for inhibition by maleimides but not catalysis.
Topics: Alanine; Amino Acid Sequence; Arabidopsis; Base Sequence; Catalysis; Cysteine; DNA, Complementary; Ethylmaleimide; Molecular Sequence Data; Mutagenesis, Site-Directed; Pyrophosphatases; Saccharomyces cerevisiae; Serine; Vacuoles | 1995 |
The catalytic site of monogalactosyldiacylglycerol synthase from spinach chloroplast envelope membranes. Biochemical analysis of the structure and of the metal content.
Topics: Binding Sites; Cations, Divalent; Chelating Agents; Chlorides; Chloroplasts; Cysteine; Dithiothreitol; Ethylmaleimide; Galactosyltransferases; Histidine; Intracellular Membranes; Kinetics; Molecular Weight; Spinacia oleracea; Sulfur Radioisotopes; Uridine Diphosphate; Zinc; Zinc Compounds | 1995 |
Functional analysis of conserved cysteine residues in the catalytic subunit of the yeast vacuolar H(+)-ATPase.
Topics: Base Sequence; Binding Sites; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Molecular Sequence Data; Mutagenesis, Site-Directed; Nitrates; Proton-Translocating ATPases; Saccharomyces cerevisiae; Sequence Alignment; Serine | 1994 |
A conformational change in the lactose permease of Escherichia coli is induced by ligand binding or membrane potential.
Topics: Cysteine; Electrochemistry; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Fluorescence; Galactosides; Kinetics; Lactose; Ligands; Liposomes; Maleimides; Membrane Potentials; Membrane Transport Proteins; Monosaccharide Transport Proteins; Protein Conformation; Spectrometry, Fluorescence; Substrate Specificity; Sulfhydryl Compounds; Symporters; Valine | 1994 |
N-ethylmaleimide-sensitive mutant (beta Val-153-->Cys) Escherichia coli F1-ATPase: cross-linking of the mutant beta subunit with the alpha subunit.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Cross-Linking Reagents; Cysteine; Escherichia coli; Ethylmaleimide; Molecular Sequence Data; Mutation; Proton-Translocating ATPases; Succinimides | 1994 |
Hot spots for sulfhydryl inactivation of Cys mutants in the widely conserved sequence motifs of the metal-tetracycline/H+ antiporter of Escherichia coli.
Topics: Amino Acid Sequence; Bacterial Proteins; Base Sequence; Conserved Sequence; Cysteine; Escherichia coli; Ethylmaleimide; Helix-Loop-Helix Motifs; Metals; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Secondary; Repressor Proteins; Sulfhydryl Reagents; Tetracycline | 1994 |
Identification of a conserved oxidation-sensitive cysteine residue in the NFI family of DNA-binding proteins.
Topics: Amino Acid Sequence; Base Sequence; CCAAT-Enhancer-Binding Proteins; Conserved Sequence; Cysteine; DNA-Binding Proteins; DNA, Complementary; Ethylmaleimide; Humans; Molecular Sequence Data; NFI Transcription Factors; Nuclear Proteins; Oxidation-Reduction; Transcription Factors; Y-Box-Binding Protein 1 | 1994 |
Investigation of the nature of the two metal-binding sites in 5-aminolaevulinic acid dehydratase from Escherichia coli.
Topics: Amino Acid Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cobalt; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Ethylmaleimide; Hydrogen-Ion Concentration; Magnesium; Metals; Molecular Sequence Data; Peptide Mapping; Porphobilinogen Synthase; Spectrometry, Fluorescence; Spectrophotometry, Atomic; Zinc | 1994 |
Investigation of ionizable residues critical for sequence-specific enzymatic DNA modification: protein modification and steady-state and pre-steady-state kinetic pH analyses of EcoRI DNA methyltransferase.
Topics: Base Sequence; Cysteine; DNA; Ethylmaleimide; Hydrogen-Ion Concentration; Ions; Kinetics; Molecular Sequence Data; Oligodeoxyribonucleotides; Proteins; Site-Specific DNA-Methyltransferase (Adenine-Specific); Spectrometry, Fluorescence | 1994 |
Cysteine scanning mutagenesis of the N-terminal 32 amino acid residues in the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Base Sequence; Biological Transport, Active; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Kinetics; Lactose; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Structure-Activity Relationship; Symporters | 1994 |
Effects of divalent cations bound to the catalytic site on ATP-induced conformational changes in the sarcoplasmic reticulum Ca(2+)-ATPase: stopped-flow analysis of the fluorescence of N-acetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine attached to cysteine-67
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; Calcium-Transporting ATPases; Catalysis; Cations, Divalent; Cysteine; Ethylmaleimide; Fluorescent Dyes; Kinetics; Muscles; Naphthalenesulfonates; Protein Conformation; Rabbits; Sarcoplasmic Reticulum; Spectrometry, Fluorescence | 1994 |
Non-adrenergic, non-cholinergic relaxation of the bovine retractor penis muscle: role of S-nitrosothiols.
Topics: Animals; Cattle; Cysteine; Ethylmaleimide; Glutathione; Hydrogen-Ion Concentration; Hydroquinones; Male; Muscle Relaxation; Nitric Oxide; Nitrogen Dioxide; Nitroso Compounds; Penis; Pyrogallol; Rabbits; S-Nitrosoglutathione; S-Nitrosothiols | 1994 |
Alkylation of cysteine 89 of the gamma subunit of chloroplast coupling factor 1 with N-ethylmaleimide alters nucleotide interactions.
Topics: Adenine Nucleotides; Adenosine Diphosphate; Adenylyl Imidodiphosphate; Alkylation; Binding Sites; Chloroplasts; Cysteine; Ethylmaleimide; Intracellular Membranes; Kinetics; Oxidation-Reduction; Phosphorylation; Photochemistry; Proton-Translocating ATPases | 1994 |
Photoactivation of rhodopsin involves alterations in cysteine side chains: detection of an S-H band in the Meta I-->Meta II FTIR difference spectrum.
Topics: Amino Acid Sequence; Animals; Cattle; Cysteine; Disulfides; Ethylmaleimide; Rhodopsin; Rod Cell Outer Segment; Spectroscopy, Fourier Transform Infrared | 1994 |
Molecular organization at the glycoprotein-complex-binding site of dystrophin. Three dystrophin-associated proteins bind directly to the carboxy-terminal portion of dystrophin.
Topics: Antibodies; Antibodies, Monoclonal; Binding Sites; Cysteine; Dystrophin; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Glycoproteins; Humans; Immunoblotting; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Fusion Proteins; Restriction Mapping; Sarcolemma | 1994 |
Oxidation of critical cysteine residues of type I adenylyl cyclase by o-iodosobenzoate or nitric oxide reversibly inhibits stimulation by calcium and calmodulin.
Topics: Adenylyl Cyclase Inhibitors; Adenylyl Cyclases; Animals; Calcium; Calmodulin; Cattle; Cells, Cultured; Cysteine; Enzyme Activation; Ethylmaleimide; Iodobenzoates; Kinetics; Nitric Oxide; Nitroprusside; Sulfhydryl Compounds | 1994 |
Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Erythrocytes; Ethylmaleimide; Humans; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; Peptide Fragments; Phosphoric Monoester Hydrolases; Protein Binding; Sulfhydryl Reagents | 1994 |
The alpha/beta subunit interaction in H(+)-ATPase (ATP synthase). An Escherichia coli alpha subunit mutation (Arg-alpha 296-->Cys) restores coupling efficiency to the deleterious beta subunit mutant (Ser-beta 174-->Phe).
Topics: Amino Acid Sequence; Arginine; Biological Transport; Catalysis; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Hydrogen; Molecular Sequence Data; Mutation; Phenylalanine; Proton-Translocating ATPases; Serine | 1994 |
Inhibition of vacuolar H(+)-ATPase by disulfide bond formation between cysteine 254 and cysteine 532 in subunit A.
Topics: Amino Acid Sequence; Animals; Cattle; Cysteine; Disulfides; Ethylmaleimide; Hydrogen-Ion Concentration; Molecular Sequence Data; Proton-Translocating ATPases; Vacuoles | 1994 |
Biotinylated and cysteine-modified peptides as useful reagents for studying the inhibition of cathepsin G.
Topics: alpha 1-Antichymotrypsin; Amino Acid Sequence; Biotin; Cathepsin G; Cathepsins; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Peptides; Reproducibility of Results; Sensitivity and Specificity; Serine Endopeptidases; Tritium | 1994 |
Identification of the essential cysteine residue in the active site of bovine pyruvate dehydrogenase.
Topics: Amino Acid Sequence; Animals; Binding Sites; Carbon Radioisotopes; Cattle; Chromatography, High Pressure Liquid; Cysteine; Ethylmaleimide; Kidney; Kinetics; Macromolecular Substances; Molecular Sequence Data; Peptide Fragments; Pyruvate Dehydrogenase Complex; Thiamine Pyrophosphate | 1993 |
Inhibition of outer hair cell electromotility by sulfhydryl specific reagents.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cell Movement; Cysteine; Cystine; Diamide; Electric Stimulation; Ethacrynic Acid; Ethylmaleimide; Guinea Pigs; Hair Cells, Auditory, Outer; Membrane Proteins; Mersalyl; Nerve Tissue Proteins; Protein Conformation; Sulfhydryl Reagents | 1993 |
Cysteine scanning mutagenesis of putative helix XI in the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Base Sequence; Biological Transport, Active; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Lactose; Membrane Transport Proteins; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Analysis, DNA; Structure-Activity Relationship; Symporters | 1993 |
Expression and characterization of recombinant human and rat liver 6-pyruvoyl tetrahydropterin synthase. Modified cysteine residues inhibit the enzyme activity.
Topics: Alcohol Oxidoreductases; Animals; Base Sequence; Chromatography, Gel; Cloning, Molecular; Cysteine; DNA Primers; Escherichia coli; Ethylmaleimide; Factor Xa; Gene Expression; Genetic Vectors; Guanidine; Guanidines; Humans; Kinetics; Liver; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphorus-Oxygen Lyases; Pyridines; Rats; Recombinant Proteins; Restriction Mapping | 1994 |
Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Base Sequence; Biological Transport, Active; Cysteine; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Kinetics; Lactose; Membrane Transport Proteins; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Symporters | 1993 |
Conserved cysteine residues are critical for the enzymatic function of the lymphocyte-specific tyrosine protein kinase p56lck.
Topics: Alanine; Amino Acid Sequence; Animals; Base Sequence; Conserved Sequence; Cysteine; DNA; Ethylmaleimide; Lymphocyte Specific Protein Tyrosine Kinase p56(lck); Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein-Tyrosine Kinases | 1993 |
Modification of a free Fe-S cluster cysteine residue in the active iron-responsive element-binding protein prevents RNA binding.
Topics: Amino Acid Sequence; Animals; Cell Line; Cysteine; Cytosol; Deferoxamine; Ethylmaleimide; Fibroblasts; Hemin; Iron-Regulatory Proteins; Iron-Sulfur Proteins; Mice; Polymerase Chain Reaction; Recombinant Proteins; RNA-Binding Proteins; RNA, Messenger; Serine | 1993 |
Role of the His-Cys finger of Moloney murine leukemia virus integrase protein in integration and disintegration.
Topics: Amino Acid Sequence; Base Sequence; Cysteine; Deoxyribonuclease I; DNA Nucleotidyltransferases; DNA, Viral; Ethylmaleimide; Histidine; Integrases; Kinetics; Models, Structural; Molecular Sequence Data; Moloney murine leukemia virus; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Recombinant Proteins; Repetitive Sequences, Nucleic Acid; Substrate Specificity; Virus Integration | 1993 |
Peptide binding to protein disulfide isomerase occurs at a site distinct from the active sites.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cadmium; Chromatography, High Pressure Liquid; Cyanogen Bromide; Cysteine; Ethylmaleimide; Iodine Radioisotopes; Isomerases; Kinetics; Liver; Male; Molecular Sequence Data; Peptide Fragments; Peptides; Protein Disulfide-Isomerases; Rats; Rats, Sprague-Dawley; Thioredoxins; Trypsin | 1993 |
Effects of sulfhydryl reagents on the Cys65 mutant of the transposon Tn10-encoded metal-tetracycline/H+ antiporter of Escherichia coli.
Topics: Amino Acid Sequence; Antiporters; Aspartic Acid; Bacterial Proteins; Carrier Proteins; Cysteine; DNA Transposable Elements; Escherichia coli; Ethylmaleimide; Methyl Methanesulfonate; Models, Molecular; Molecular Sequence Data; Mutation; Protein Conformation; Repressor Proteins; Structure-Activity Relationship; Sulfhydryl Reagents; Tetracycline; Tetracycline Resistance | 1993 |
Modification of Cys-128 of pig kidney fructose 1,6-bisphosphatase with different thiol reagents: size dependent effect on the substrate and fructose-2,6-bisphosphate interaction.
Topics: Amino Acids; Animals; Cysteine; Ethylmaleimide; Fructose-Bisphosphatase; Fructosediphosphates; Kidney Cortex; Kinetics; Spectrophotometry, Ultraviolet; Substrate Specificity; Swine | 1993 |
Accessibility of hepatocyte protein thiols to monobromobimane.
Topics: Amino Acids; Animals; Benzoquinones; Bridged Bicyclo Compounds; Cells, Cultured; Cysteine; Cytosol; Ethylmaleimide; Fluorescent Dyes; Liver; Male; Microsomes, Liver; Mitochondria, Liver; Proteins; Rats; Rats, Wistar; Serum Albumin, Bovine; Sulfhydryl Compounds; Time Factors | 1993 |
The specific interactions of HMG 1 and 2 with negatively supercoiled DNA are modulated by their acidic C-terminal domains and involve cysteine residues in their HMG 1/2 boxes.
Topics: Animals; Cattle; Cysteine; DNA-Binding Proteins; DNA, Single-Stranded; DNA, Superhelical; Ethylmaleimide; High Mobility Group Proteins; In Vitro Techniques; Peptide Fragments; Plasmids; Protein Binding; Structure-Activity Relationship | 1993 |
The cysteine introduced into the alpha subunit of the Escherichia coli F1-ATPase by the mutation alpha R376C is near the alpha-beta subunit interface and close to a noncatalytic nucleotide binding site.
Topics: Adenosine Triphosphate; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Fluorescence; Immunoblotting; Kinetics; Mutagenesis; Proton-Translocating ATPases | 1993 |
Cysteine desulfurase activity indicates a role for NIFS in metallocluster biosynthesis.
Topics: Amino Acid Sequence; Azotobacter vinelandii; Bacterial Proteins; Carbon-Sulfur Lyases; Chromatography, Ion Exchange; Cloning, Molecular; Cysteine; DNA, Bacterial; Escherichia coli; Ethylmaleimide; Genes, Bacterial; Kinetics; Lyases; Molecular Sequence Data; Molecular Weight; Multigene Family; Nitrogen Fixation; Sequence Homology, Amino Acid; Spectrophotometry | 1993 |
Chemical modification of rat liver cytosolic NADP(+)-linked isocitrate dehydrogenase by N-ethylmaleimide. Evidence for essential sulphydryl groups.
Topics: Animals; Cysteine; Cytosol; Enzyme Activation; Ethylmaleimide; Hydrogen-Ion Concentration; Isocitrate Dehydrogenase; Kinetics; Liver; Molecular Conformation; Rats; Sulfhydryl Compounds | 1993 |
Chemical modifications of chicken liver pyruvate carboxylase: evidence for essential cysteine-lysine pairs and a reactive sulfhydryl group.
Topics: Adenosine Diphosphate; Animals; Binding Sites; Carbamyl Phosphate; Chickens; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Liver; Lysine; Maleimides; o-Phthalaldehyde; Oxaloacetates; Phosphorylation; Pyruvate Carboxylase; Structure-Activity Relationship; Sulfhydryl Compounds | 1993 |
Cross-link between cys 374 and cys 10 of actin abolishes polymerizability and allows study of the properties of the "F-actin monomer".
Topics: Actins; Amino Acid Sequence; Animals; Binding Sites; Chromatography, Gel; Crystallography, X-Ray; Cyanogen Bromide; Cysteine; Deoxyribonuclease I; Disulfides; Ethylmaleimide; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Kinetics; Macromolecular Substances; Microfilament Proteins; Myosin Subfragments; Myosins; Peptide Fragments; Peptide Mapping; Phalloidine; Potassium Chloride; Protein Conformation; Spectrometry, Fluorescence | 1996 |
Mechanisms for cytoprotection by vitamin U from ethanol-induced gastric mucosal damage in rats.
Topics: Animals; Anti-Ulcer Agents; Cysteine; Ethanol; Ethylmaleimide; Gastric Mucosa; Male; Mucins; Rats; Rats, Wistar; Sulfhydryl Reagents; Vitamin U | 1996 |
Cysteine-scanning mutagenesis of helix VI and the flanking hydrophilic domains on the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Bacterial Proteins; Biological Transport, Active; Cell Compartmentation; Cysteine; DNA Mutational Analysis; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Lactose; Membrane Proteins; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Protein Structure, Secondary; Sulfhydryl Reagents; Symporters | 1996 |
Diallyl disulfide inhibits the proliferation of human tumor cells in culture.
Topics: Allyl Compounds; Antineoplastic Agents; Calcium; Calcium-Transporting ATPases; Cell Division; Cysteine; Disulfides; Egtazic Acid; Ethylmaleimide; Garlic; Humans; Kinetics; Molecular Structure; Neoplasms; Plants, Medicinal; Tumor Cells, Cultured | 1996 |
Identification of the active-site peptide of 2,3-dihydroxybenzoic acid decarboxylase from Aspergillus oryzae.
Topics: Amino Acid Sequence; Aspergillus oryzae; Benzoates; Binding Sites; Carboxy-Lyases; Catalysis; Cysteine; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Histidine; Hydrogen-Ion Concentration; Indoles; Kinetics; Molecular Sequence Data; Molecular Structure; Peptide Fragments; Salicylates; Salicylic Acid; Spectrophotometry, Ultraviolet | 1996 |
Characterization of the interface between gamma and epsilon subunits of Escherichia coli F1-ATPase.
Topics: Amino Acid Sequence; Binding Sites; Cross-Linking Reagents; Cysteine; Escherichia coli; Ethylmaleimide; Helix-Loop-Helix Motifs; Kinetics; Macromolecular Substances; Models, Structural; Molecular Sequence Data; Mutagenesis, Site-Directed; Point Mutation; Protein Structure, Secondary; Proton-Translocating ATPases; Recombinant Proteins; Structure-Activity Relationship | 1996 |
Sulfhydryl redox modulates ATP-sensitive K+ channels in rabbit ventricular myocytes.
Topics: Adenosine Triphosphate; Animals; Cells, Cultured; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Glyburide; Heart; Heart Ventricles; Ion Channel Gating; Kinetics; Membrane Potentials; Oxidation-Reduction; Patch-Clamp Techniques; Potassium Channels; Rabbits; Sulfhydryl Reagents; Time Factors | 1996 |
Inactivation of cholesteryl ester transfer protein by cysteine modification.
Topics: 4-Chloromercuribenzenesulfonate; Alkylating Agents; Animals; Azides; Carrier Proteins; Cell Line; Cholesterol Ester Transfer Proteins; Cholesterol Esters; Cricetinae; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Glycoproteins; Humans; Iodoacetamide; Kinetics; Pyridines; Recombinant Proteins; Structure-Activity Relationship; Sulfhydryl Reagents; Transfection | 1996 |
Thiol modification and site directed mutagenesis of the flavin domain of spinach NADH:nitrate reductase.
Topics: Amino Acid Sequence; Base Sequence; Circular Dichroism; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Ferricyanides; Flavin-Adenine Dinucleotide; Flavins; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Nitrate Reductase (NADH); Nitrate Reductases; Oxidation-Reduction; Spectrophotometry; Spinacia oleracea; Structure-Activity Relationship; Sulfhydryl Reagents | 1996 |
A conserved cysteine residue in the runt homology domain of AML1 is required for the DNA binding ability and the transforming activity on fibroblasts.
Topics: 3T3 Cells; Animals; Cell Line; Cell Transformation, Neoplastic; Conserved Sequence; Core Binding Factor Alpha 2 Subunit; Cysteine; Diamide; DNA-Binding Proteins; Ethylmaleimide; Fibroblasts; Mice; Mutagenesis, Site-Directed; Neoplasm Proteins; Oxidation-Reduction; Protein Binding; Proto-Oncogene Proteins; Serine; Transcription Factor AP-2; Transcription Factors; Transcriptional Activation | 1996 |
Probing the conformation of the lactose permease of Escherichia coli by in situ site-directed sulfhydryl modification.
Topics: Amino Acid Sequence; Biotin; Carbohydrate Sequence; Cell Membrane; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Kinetics; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Plasmids; Point Mutation; Protein Conformation; Recombinant Proteins; Sulfhydryl Compounds; Symporters; Thiogalactosides; Valine | 1996 |
Evidence for the presence of essential histidine and cysteine residues in platelet cGMP-inhibited phosphodiesterase.
Topics: 3',5'-Cyclic-AMP Phosphodiesterases; Adenosine Monophosphate; Binding Sites; Blood Platelets; Catalysis; Cyclic AMP; Cyclic GMP; Cyclic Nucleotide Phosphodiesterases, Type 3; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Ethylmaleimide; Histidine; Humans; Kinetics; Phosphodiesterase Inhibitors | 1996 |
Phosphorylation of phospholamban by cAMP-dependent protein kinase enhances interactions between Ca-ATPase polypeptide chains in cardiac sarcoplasmic reticulum membranes.
Topics: Animals; Calcium; Calcium-Binding Proteins; Calcium-Transporting ATPases; Cyclic AMP-Dependent Protein Kinases; Cysteine; Electron Spin Resonance Spectroscopy; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Ethylmaleimide; Lipid Metabolism; Myocardium; Phosphorylation; Rabbits; Sarcoplasmic Reticulum; Spin Labels; Stearic Acids; Thermodynamics | 1996 |
The function and properties of the iron-sulfur center in spinach ferredoxin: thioredoxin reductase: a new biological role for iron-sulfur clusters.
Topics: Chloroplasts; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electron Spin Resonance Spectroscopy; Electron Transport; Ethylmaleimide; Ferredoxins; Ferricyanides; Iron; Iron-Sulfur Proteins; NADP; Oxidoreductases; Spectrophotometry; Spectrum Analysis, Raman; Spinacia oleracea; Sulfur | 1996 |
Site-directed fluorescence labeling of P-glycoprotein on cysteine residues in the nucleotide binding domains.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Anilino Naphthalenesulfonates; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Binding, Competitive; Biological Transport; CHO Cells; Cricetinae; Cysteine; Drug Resistance, Multiple; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Ethylmaleimide; Fluorescent Dyes; Kinetics; Molecular Sequence Data; Nucleotides; Spectrometry, Fluorescence; Sulfhydryl Reagents | 1996 |
Nitric oxide inhibits aromatase activity: mechanisms of action.
Topics: Androstenedione; Aromatase Inhibitors; Carbon Monoxide; Cells, Cultured; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Female; Gene Expression Regulation, Enzymologic; Granulosa Cells; Humans; Luteal Cells; Mercaptoethanol; Microsomes; NADP; Nitric Oxide; Penicillamine; Placenta; RNA, Messenger; S-Nitroso-N-Acetylpenicillamine; Sulfhydryl Reagents | 1996 |
Redox regulation of GA-binding protein-alpha DNA binding activity.
Topics: 3T3 Cells; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Cysteine; DNA-Binding Proteins; Ethylmaleimide; GA-Binding Protein Transcription Factor; Glutathione; Glutathione Disulfide; Homeostasis; Humans; Macromolecular Substances; Mice; Molecular Sequence Data; Oxidation-Reduction; Promoter Regions, Genetic; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-ets; Recombinant Proteins; Transcription Factors | 1996 |
Site-directed spin labeling and chemical crosslinking demonstrate that helix V is close to helices VII and VIII in the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Biotin; Cell Membrane; Cysteine; Cytoplasm; Electron Spin Resonance Spectroscopy; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Transport Proteins; Models, Structural; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Recombinant Proteins; Spin Labels; Symporters | 1996 |
The Escherichia coli F1-ATPase mutant beta Tyr-297-->Cys: functional studies and asymmetry of the enzyme under various nucleotide conditions based on reaction of the introduced Cys with N-ethylmaleimide and 7-chloro-4-nitrobenzofurazan.
Topics: Benzofurans; Binding Sites; Copper; Cross-Linking Reagents; Cysteine; Escherichia coli; Ethylmaleimide; Mutation; Nucleotides; Oxidative Phosphorylation; Protein Conformation; Proton-Translocating ATPases; Sulfhydryl Reagents | 1996 |
Structure and function in rhodopsin. Single cysteine substitution mutants in the cytoplasmic interhelical E-F loop region show position-specific effects in transducin activation.
Topics: Amino Acid Sequence; Animals; Cattle; COS Cells; Cysteine; Ethylmaleimide; Fluorescence; Gene Expression; Guanosine Triphosphate; Light; Molecular Sequence Data; Mutagenesis; Recombinant Proteins; Rhodopsin; Rod Opsins; Spectrophotometry; Transducin | 1996 |
Mutations in a putative zinc-binding domain inactivate the mitochondrial intermediate peptidase.
Topics: Amino Acid Sequence; Binding Sites; Cloning, Molecular; Codon; Conserved Sequence; Cysteine; DNA Mutational Analysis; Enzyme Stability; Escherichia coli; Ethylmaleimide; Iodoacetates; Iodoacetic Acid; Metalloendopeptidases; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxygen Consumption; Protein Precursors; Recombinant Fusion Proteins; Restriction Mapping; Saccharomyces cerevisiae; Zinc Fingers | 1996 |
Formation of protein-glutathione mixed disulfides in the developing rat conceptus following diamide treatment in vitro.
Topics: Animals; Cysteine; Diamide; Dithiothreitol; Dose-Response Relationship, Drug; Embryo, Mammalian; Embryonic and Fetal Development; Ethylmaleimide; Female; Glutathione; Organ Culture Techniques; Pregnancy; Protein Disulfide Reductase (Glutathione); Rats; Rats, Sprague-Dawley; Sulfhydryl Reagents; Tissue Distribution | 1995 |
Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of EScherichia coli.
Topics: Amino Acid Sequence; Biological Transport; Blotting, Western; Cell Membrane; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Lactose; Membrane Transport Proteins; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Symporters | 1996 |
Mutational analysis and chemical modification of Cys24 of lactococcin B, a bacteriocin produced by Lactococcus lactis.
Topics: Bacteriocins; Copper Sulfate; Cysteine; Dithiothreitol; Ethylmaleimide; Formates; Lactococcus lactis; Membrane Potentials; Mercuribenzoates; Mercuric Chloride; Mutagenesis, Site-Directed; Oxidants; Oxidation-Reduction; Reducing Agents | 1996 |
Quality control in protein biogenesis: thiol-mediated retention monitors the redox state of proteins in the endoplasmic reticulum.
Topics: Alkylation; Animals; Cysteine; Endoplasmic Reticulum; Ethylmaleimide; Hexosaminidases; Hybridomas; Immunoglobulin M; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Oxidation-Reduction; Protein Biosynthesis; Proteins; Quality Control; Sulfhydryl Compounds | 1996 |
Intermolecular coupling between loop 38-52 and the C-terminus in actin filaments.
Topics: Actins; Animals; Binding Sites; Ca(2+) Mg(2+)-ATPase; Copper; Cysteine; Dansyl Compounds; Ethylenediamines; Ethylmaleimide; Fluorescent Dyes; Kinetics; Muscle, Skeletal; Peptide Fragments; Rabbits; Spectrometry, Fluorescence | 1996 |
Identification of a cysteine residue essential for activity of protein farnesyltransferase. Cys299 is exposed only upon removal of zinc from the enzyme.
Topics: Alkyl and Aryl Transferases; Chromatography, Ion Exchange; Cysteine; Dimethylallyltranstransferase; Dithiothreitol; Ethylmaleimide; Kinetics; Mutagenesis, Site-Directed; Structure-Activity Relationship; Sulfhydryl Reagents; Transferases; Trypsin; Zinc | 1996 |
Specificity of aspartokinase III from Escherichia coli and an examination of important catalytic residues.
Topics: Aspartate Kinase; Binding Sites; Chromatography, Ion Exchange; Cloning, Molecular; Cysteine; Enzyme Inhibitors; Escherichia coli; Ethylmaleimide; Hydrogen-Ion Concentration; Imidazoles; Kinetics; Macromolecular Substances; Recombinant Proteins; Substrate Specificity; Tetranitromethane | 1996 |
Identification and characterization of the N-ethylmaleimide-sensitive site in lambda-integrase.
Topics: Amino Acid Sequence; Bacteriophage lambda; Cysteine; Ethylmaleimide; Integrase Inhibitors; Integrases; Molecular Sequence Data; Mutagenesis, Site-Directed | 1996 |
Specific cardiolipin binding interferes with labeling of sulfhydryl residues in the adenosine diphosphate/adenosine triphosphate carrier protein from beef heart mitochondria.
Topics: Animals; Cardiolipins; Carrier Proteins; Cattle; Cysteine; Ethylmaleimide; Fluorescence; Fluorescent Dyes; Kinetics; Magnetic Resonance Spectroscopy; Maleimides; Mitochondria, Heart; Phospholipases A; Phospholipases A2; Phospholipids; Protein Binding; Protein Conformation; Receptors, Cytoplasmic and Nuclear; Sulfhydryl Compounds; Sulfhydryl Reagents | 1996 |
Determination of a transmembrane segment using cysteine-scanning mutants of transposon Tn10-encoded metal-tetracycline/H+ antiporter.
Topics: Amino Acid Sequence; Antiporters; Bacterial Proteins; Cell Membrane; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethylmaleimide; Membrane Proteins; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Protein Binding; Tetracycline Resistance | 1996 |
Mutational analysis of human uroporphyrinogen decarboxylase.
Topics: Amino Acid Sequence; Animals; Cysteine; Ethylmaleimide; Histidine; Humans; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Rats; Recombinant Proteins; Sequence Homology, Amino Acid; Uroporphyrinogen Decarboxylase | 1996 |
Nitric oxide-related species inhibit evoked neurotransmission but enhance spontaneous miniature synaptic currents in central neuronal cultures.
Topics: Animals; Aplysia; Cells, Cultured; Cerebral Cortex; Cyclic GMP; Cysteine; Ethylmaleimide; Fetus; Hippocampus; Hydrazines; Kinetics; Neuroglia; Neurons; Nitric Oxide; Nitrogen Oxides; Nitroglycerin; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; S-Nitrosothiols; Synapses; Synaptic Transmission | 1996 |
Cysteine-scanning mutagenesis of helix II and flanking hydrophilic domains in the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Carrier Proteins; Conserved Sequence; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Lactose; Membrane Proteins; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Mutation; Protein Structure, Secondary; Symporters | 1997 |
Membrane topology of the transposon 10-encoded metal-tetracycline/H+ antiporter as studied by site-directed chemical labeling.
Topics: Amino Acid Sequence; Antiporters; Bacterial Proteins; Cysteine; DNA Transposable Elements; Ethylmaleimide; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Plasmids; Sulfhydryl Reagents; Tetracycline Resistance | 1997 |
Identification of sulfhydryl-modified cysteine residues in the ligand binding pocket of retinoic acid receptor beta.
Topics: Binding Sites; Blotting, Western; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Mutagenesis, Site-Directed; Plasmids; Polymerase Chain Reaction; Protease Inhibitors; Receptors, Retinoic Acid; Retinoic Acid Receptor alpha; Retinoic Acid Receptor gamma; Structure-Activity Relationship; Sulfhydryl Reagents; Tretinoin | 1997 |
Reactive cysteines of the yeast plasma-membrane H+-ATPase (PMA1). Mapping the sites of inactivation by N-ethylmaleimide.
Topics: Cell Membrane; Cysteine; Ethylmaleimide; Mutagenesis, Site-Directed; Proton-Translocating ATPases; Saccharomyces cerevisiae | 1997 |
Identification of an essential cysteine residue in human glutathione synthase.
Topics: Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Glutathione Synthase; Humans; Iodoacetates; Iodoacetic Acid; Mutagenesis, Site-Directed; Phencyclidine | 1997 |
The role of helix VIII in the lactose permease of Escherichia coli: I. Cys-scanning mutagenesis.
Topics: Amino Acid Sequence; Biological Transport, Active; Cysteine; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Transport Modulators; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Symporters | 1997 |
Chemical modification of an alpha 3-fucosyltransferase; definition of amino acid residues essential for enzyme activity.
Topics: Amino Acid Sequence; Cysteine; Diethyl Pyrocarbonate; Ethylmaleimide; Fucosyltransferases; Guanosine Diphosphate Fucose; Histidine; Humans; Molecular Sequence Data; Sequence Alignment | 1997 |
Resonance energy transfer between sites in rat liver glutathione S-transferase, 1-1, selectively modified at cysteine-17 and cysteine-111.
Topics: Amino Acid Sequence; Animals; Bridged Bicyclo Compounds; Cysteine; Energy Transfer; Enzyme Activation; Ethylmaleimide; Fluoresceins; Fluorescence Polarization; Fluorescent Dyes; Glutathione Transferase; Kinetics; Liver; Peptide Fragments; Rats; Spectrometry, Fluorescence; Sulfhydryl Reagents; Thermolysin | 1997 |
Role of cysteine residues of rat A2a adenosine receptors in agonist binding.
Topics: Adenosine; Adenosine-5'-(N-ethylcarboxamide); Animals; Cell Membrane; Corpus Striatum; Cysteine; Dithiothreitol; Ethylmaleimide; Guanosine 5'-O-(3-Thiotriphosphate); Guanylyl Imidodiphosphate; Male; Phenethylamines; Purinergic P1 Receptor Agonists; Purinergic P1 Receptor Antagonists; Radioligand Assay; Rats; Rats, Sprague-Dawley; Receptors, Purinergic P1; Sulfhydryl Reagents | 1997 |
Yeast plasma-membrane H(+)-ATPase: the role of cysteine residues.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Alanine; Chromosome Mapping; Cloning, Molecular; Cysteine; DNA, Fungal; Ethylmaleimide; Gene Expression Regulation, Fungal; Immunoblotting; Mutagenesis, Site-Directed; Proton Pumps; Proton-Translocating ATPases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins | 1996 |
Interactions between the F1 and F0 parts in the Escherichia coli ATP synthase. Associations involving the loop region of C subunits.
Topics: Cysteine; Escherichia coli; Ethylmaleimide; Kinetics; Mutagenesis; Proton-Translocating ATPases | 1997 |
Identification in the mu-opioid receptor of cysteine residues responsible for inactivation of ligand binding by thiol alkylating and reducing agents.
Topics: Alkylating Agents; Alkylation; Animals; Binding Sites; Cloning, Molecular; COS Cells; Cysteine; Diprenorphine; Disulfides; Dithiothreitol; Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; Enkephalins; Ethylmaleimide; Humans; Ligands; Mutagenesis, Site-Directed; Narcotic Antagonists; Protein Binding; Receptors, Opioid, mu; Reducing Agents; Sulfhydryl Reagents; Transfection | 1997 |
Subunit-specific mutagenesis of the cysteine 280 residue of the reverse transcriptase of human immunodeficiency virus type 1: effects on sensitivity to a specific inhibitor of the RNase H activity.
Topics: Binding Sites; Cysteine; Enzyme Inhibitors; Ethylmaleimide; HIV Reverse Transcriptase; Humans; Molecular Structure; Mutagenesis; Ribonuclease H | 1997 |
An ADP-ribosyltransferase from bovine erythrocytes apparently specific for cysteine residues.
Topics: Adenosine Diphosphate Ribose; Animals; Cattle; Cysteine; Enzyme Inhibitors; Erythrocytes; Ethylmaleimide; NAD; NAD+ Nucleosidase; Poly(ADP-ribose) Polymerases; Substrate Specificity | 1997 |
Activation of the leukocyte NADPH oxidase subunit p47phox by protein kinase C. A phosphorylation-dependent change in the conformation of the C-terminal end of p47phox.
Topics: Amino Acid Sequence; Cyanogen Bromide; Cysteine; Enzyme Activation; Ethylmaleimide; Humans; Molecular Sequence Data; NADPH Oxidases; Neutrophils; Peptide Fragments; Phosphoproteins; Phosphorylation; Protein Conformation; Protein Kinase C; Structure-Activity Relationship | 1997 |
Inactivation of yeast glutathione reductase by O-phthalaldehyde.
Topics: Cysteine; Enzyme Activation; Ethylmaleimide; Glutathione Reductase; Lysine; o-Phthalaldehyde; Spectrometry, Fluorescence; Substrate Specificity; Sulfhydryl Reagents; Yeasts | 1996 |
Possible thiol group involvement in intracellular pH effect on low-conductance Ca(2+)-dependent K+ channels.
Topics: Calcium; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Electric Stimulation; Ethylmaleimide; HeLa Cells; Humans; Hydrogen-Ion Concentration; Intracellular Fluid; Ion Channel Gating; Kinetics; Membrane Potentials; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Probability; Sulfhydryl Reagents; Time Factors | 1997 |
Covalent modification of the regulatory domain irreversibly stimulates cystic fibrosis transmembrane conductance regulator.
Topics: Adenosine Triphosphate; Alanine; Binding Sites; Cyclic AMP-Dependent Protein Kinases; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Cytoplasm; Ethylmaleimide; Humans; Models, Chemical; Mutagenesis, Site-Directed; Transfection | 1997 |
The construction of a cysteine-less melibiose carrier from E. coli.
Topics: Amino Acid Substitution; Cell Membrane; Cysteine; DNA Primers; Escherichia coli; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Melibiose; Membrane Transport Proteins; Models, Structural; Mutagenesis, Site-Directed; Protein Structure, Secondary; Recombinant Proteins; Serine; Symporters; Valine | 1997 |
Site-directed mutagenesis of the Cys residues in ClpA, the ATPase component of protease Ti (ClpAP) in Escherichia coli.
Topics: Adenosine Triphosphatases; Alanine; Caseins; Cysteine; Electrophoresis, Polyacrylamide Gel; Endopeptidase Clp; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Mutagenesis, Site-Directed; Serine; Serine Endopeptidases; Sulfhydryl Reagents | 1997 |
Gly166 in the NK1 receptor regulates tachykinin selectivity and receptor conformation.
Topics: Amino Acid Substitution; Animals; Binding Sites; CHO Cells; COS Cells; Cricetinae; Cysteine; Dithiothreitol; Ethylmaleimide; Glycine; Humans; Kinetics; Mutagenesis, Site-Directed; Neurokinin A; Neurokinin B; Protein Conformation; Rats; Receptors, Neurokinin-1; Receptors, Tachykinin; Recombinant Proteins; Substance P | 1997 |
Yeast plasma-membrane H+(-)ATPase: role of Cys-409 in interaction of the enzyme with NEM and FITC.
Topics: Binding Sites; Cell Membrane; Cysteine; Ethylmaleimide; Fluorescein-5-isothiocyanate; Protein Conformation; Proton-Translocating ATPases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins | 1997 |
Identification of cysteine and arginine residues essential for the phosphotransacetylase from Methanosarcina thermophila.
Topics: Amino Acid Sequence; Archaeal Proteins; Arginine; Bacteria, Anaerobic; Binding Sites; Coenzyme A; Cysteine; Ethylmaleimide; Methanosarcina; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphate Acetyltransferase; Pyruvaldehyde; Recombinant Proteins; Sequence Analysis; Sequence Homology, Amino Acid; Species Specificity | 1997 |
Activation of the cardiac calcium release channel (ryanodine receptor) by poly-S-nitrosylation.
Topics: Animals; Calcium; Cyclic GMP; Cysteine; Dithiothreitol; Dogs; Electric Conductivity; Ethylmaleimide; Glutathione; Liposomes; Mercaptoethanol; Molsidomine; Myocardium; Nitric Oxide; Nitrosation; Nitroso Compounds; Oxidation-Reduction; Proteolipids; Ryanodine Receptor Calcium Release Channel; S-Nitrosoglutathione; S-Nitrosothiols; Sulfhydryl Compounds | 1998 |
Identification of amino acids in the binding pocket of the human KDEL receptor.
Topics: Amino Acid Sequence; Amino Acid Substitution; Amino Acids; Binding Sites; Cysteine; Ethylmaleimide; Humans; Ligands; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Secondary; Receptors, Peptide; Sulfhydryl Reagents | 1998 |
Disruption of target DNA binding in Mu DNA transposition by alteration of position 99 in the Mu B protein.
Topics: Amino Acid Substitution; Bacteriophage mu; Binding Sites; Cysteine; DNA-Binding Proteins; Ethylmaleimide; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Plasmids; Protein Conformation; Transposases; Viral Proteins | 1998 |
Disulfide bond exchange in rhodopsin.
Topics: Animals; Cattle; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Mutagenesis; Photolysis; Protein Denaturation; Rhodopsin; Spectrophotometry; Transducin | 1998 |
Cysteine-scanning mutagenesis around transmembrane segment III of Tn10-encoded metal-tetracycline/H+ antiporter.
Topics: Amino Acid Sequence; Antiporters; Bacterial Proteins; Biological Transport; Cysteine; DNA Transposable Elements; Ethylmaleimide; Metals; Methyl Methanesulfonate; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Secondary; Protons; Sulfhydryl Reagents; Tetracyclines | 1998 |
Identification of redox-sensitive cysteines in GA-binding protein-alpha that regulate DNA binding and heterodimerization.
Topics: Alkylation; Amino Acid Sequence; Cysteine; Dimerization; DNA-Binding Proteins; Ethylmaleimide; GA-Binding Protein Transcription Factor; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Binding; Proto-Oncogene Protein c-ets-1; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-ets; Recombinant Proteins; Sequence Homology, Amino Acid; Transcription Factors | 1998 |
Role of the GroEL chaperonin intermediate domain in coupling ATP hydrolysis to polypeptide release.
Topics: Adenosine Triphosphate; Chaperonin 10; Chaperonin 60; Crystallography, X-Ray; Cysteine; Escherichia coli; Ethylmaleimide; Hydrolysis; Mutagenesis, Insertional; Polymerase Chain Reaction; Protein Conformation; Protein Folding; Structure-Activity Relationship; Sulfhydryl Reagents | 1998 |
Role of the [Fe4S4] cluster in mediating disulfide reduction in spinach ferredoxin:thioredoxin reductase.
Topics: Benzyl Viologen; Binding Sites; Cysteine; Disulfides; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Iron-Sulfur Proteins; Oxidation-Reduction; Oxidoreductases; Spectrum Analysis, Raman; Spinacia oleracea | 1998 |
The switch in the helical handedness of the histone (H3-H4)2 tetramer within a nucleoprotein particle requires a reorientation of the H3-H3 interface.
Topics: Animals; Base Sequence; Cysteine; Dithionitrobenzoic Acid; DNA; DNA Primers; DNA, Circular; Ducks; Ethylmaleimide; Histones; Iodoacetamide; Kinetics; Macromolecular Substances; Mammary Tumor Virus, Mouse; Nucleoproteins; Protein Structure, Secondary; Repetitive Sequences, Nucleic Acid; Stress, Mechanical; Sulfhydryl Reagents | 1998 |
Roles of conserved arginine residues in the metal-tetracycline/H+ antiporter of Escherichia coli.
Topics: Amino Acid Sequence; Amino Acid Substitution; Antiporters; Arginine; Aspartic Acid; Bacterial Proteins; Biological Transport; Carbon Radioisotopes; Cell Membrane; Conserved Sequence; Cysteine; Escherichia coli; Ethylmaleimide; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Tetracycline; Tetracycline Resistance | 1998 |
Enzymic, cysteine-specific ADP-ribosylation in bovine liver mitochondria.
Topics: Adenosine Diphosphate Ribose; Aldehyde Dehydrogenase; Animals; Cattle; Cysteine; Disulfides; Enzyme Inhibitors; Ethylmaleimide; Fungal Proteins; Mitochondria, Liver; NAD; NAD+ Nucleosidase; Poly(ADP-ribose) Polymerases | 1998 |
Site-directed mutagenesis and chemical modification of the two cysteine residues of the UDP-N-acetylmuramoyl:L-alanine ligase of Escherichia coli.
Topics: Alanine; Alkylation; Cysteine; Enzyme Activation; Enzyme Stability; Escherichia coli; Ethylmaleimide; Mutagenesis, Site-Directed; Peptide Mapping; Peptide Synthases; Reducing Agents | 1998 |
Cysteine scanning of the surroundings of an alkali-ion binding site of the glutamate transporter GLT-1 reveals a conformationally sensitive residue.
Topics: Amino Acid Transport System X-AG; Amino Acids; Aspartic Acid; ATP-Binding Cassette Transporters; Binding Sites; Biological Transport; Conserved Sequence; Cysteine; Ethylmaleimide; HeLa Cells; Humans; Kainic Acid; Mesylates; Mutagenesis, Site-Directed; Potassium; Protein Conformation; Sodium; Sulfhydryl Reagents | 1998 |
Nitric oxide as an autocrine regulator of sodium currents in baroreceptor neurons.
Topics: Animals; Aorta; Autocrine Communication; Cyclic GMP; Cysteine; Ditiocarb; Ethylmaleimide; Ferrous Compounds; Hemoglobins; Hydrazines; Ion Channel Gating; Neurons, Afferent; Nitric Oxide; Nitroso Compounds; Nodose Ganglion; Patch-Clamp Techniques; Pressoreceptors; Rats; Rats, Sprague-Dawley; S-Nitrosothiols; Sodium; Sodium Channels; Sulfhydryl Reagents; Tetrodotoxin | 1998 |
Three thiol groups are important for the activity of the liver microsomal glucose-6-phosphatase system. Unusual behavior of one thiol located in the glucose-6-phosphate translocase.
Topics: Animals; Binding Sites; Biological Transport; Carrier Proteins; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Glucose; Glucose-6-Phosphatase; Glucose-6-Phosphate; Kinetics; Microsomes, Liver; Molecular Sequence Data; Phosphates; Protein Binding; Protein Conformation; Rats; Rats, Wistar; Scattering, Radiation; Sulfhydryl Compounds; Vanadates | 1998 |
Effects of chemical modification of cysteines 201 and 317 of band 3 on hemolytic properties of human erythrocytes under hydrostatic pressure.
Topics: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Anion Exchange Protein 1, Erythrocyte; Anions; Cysteine; Electron Spin Resonance Spectroscopy; Electrophoresis, Polyacrylamide Gel; Erythrocyte Membrane; Ethylmaleimide; Hemolysis; Humans; Ion Transport; Pressure; Spin Labels | 1998 |
Effects of the cys mutations on structure and function of the ATP-dependent HslVU protease in Escherichia coli. The Cys287 to Val mutation in HslU uncouples the ATP-dependent proteolysis by HslvU from ATP hydrolysis.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Alanine; ATP-Dependent Proteases; Cysteine; Endopeptidases; Escherichia coli; Ethylmaleimide; Heat-Shock Proteins; Hydrolysis; Mutagenesis, Site-Directed; Protein Conformation; Serine; Serine Endopeptidases; Substrate Specificity; Valine | 1998 |
Probing the role of cysteine residues in the EcoP15I DNA methyltransferase.
Topics: Amino Acid Substitution; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; DNA Methylation; Escherichia coli; Ethylmaleimide; Iodoacetamide; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Recombinant Proteins; Site-Specific DNA-Methyltransferase (Adenine-Specific) | 1998 |
Non-equivalent cooperation between the two nucleotide-binding folds of P-glycoprotein.
Topics: Adenosine Triphosphate; ATP Binding Cassette Transporter, Subfamily B, Member 1; Cysteine; Ethylmaleimide; Humans; Protein Binding; Protein Folding; Vanadates | 1998 |
Superoxide produced in the heme pocket of the beta-chain of hemoglobin reacts with the beta-93 cysteine to produce a thiyl radical.
Topics: Cysteine; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Free Radicals; Heme; Hemoglobins; Humans; Kinetics; Macromolecular Substances; Models, Chemical; Peroxides; Spectrometry, Fluorescence; Sulfhydryl Compounds; Superoxides | 1998 |
Nitric oxide inhibition of the rat olfactory cyclic nucleotide-gated cation channel.
Topics: Animals; Cyclic AMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; Ion Channel Gating; Ion Channels; Molsidomine; Nitric Oxide Donors; Nitroso Compounds; Olfactory Receptor Neurons; Rats; S-Nitrosothiols; Sulfhydryl Reagents | 1998 |
Covalent modification of the nucleotide binding domains of cystic fibrosis transmembrane conductance regulator.
Topics: Adenosine Triphosphate; Amino Acid Sequence; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Cell Membrane; Consensus Sequence; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Ethylmaleimide; HeLa Cells; Humans; Ion Channel Gating; Membrane Potentials; Mutagenesis, Insertional; Mutagenesis, Site-Directed; Patch-Clamp Techniques; Recombinant Proteins; Transfection | 1998 |
Site-directed chemical modification of cysteine-scanning mutants as to transmembrane segment II and its flanking regions of the Tn10-encoded metal-tetracycline/H+ antiporter reveals a transmembrane water-filled channel.
Topics: Amino Acid Sequence; Antiporters; Bacterial Proteins; Biological Transport; Cysteine; DNA Transposable Elements; Escherichia coli; Ethylmaleimide; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Tetracycline; Water | 1998 |
ADP-ribosylation factor-1 is sensitive to N-ethylmaleimide.
Topics: Adaptor Protein Complex gamma Subunits; ADP-Ribosylation Factor 1; ADP-Ribosylation Factors; Animals; Cattle; Cells, Cultured; Coatomer Protein; Cysteine; Cytosol; Ethylmaleimide; Golgi Apparatus; GTP-Binding Proteins; Guanosine 5'-O-(3-Thiotriphosphate); Intracellular Membranes; Kidney; Membrane Proteins; Microtubule-Associated Proteins; Paclitaxel; Rats | 1998 |
Effect of cysteine modifications on the activity of the 'small' Clostridium perfringens sialidase.
Topics: Amino Acid Sequence; Clostridium perfringens; Cysteine; Ethylmaleimide; Gram-Positive Bacteria; Kinetics; Mercuric Chloride; Molecular Sequence Data; Mutagenesis, Site-Directed; Neuraminidase; p-Chloromercuribenzoic Acid; Recombinant Proteins; Sequence Homology, Amino Acid; Serine; Structure-Activity Relationship; Substrate Specificity | 1998 |
Formation of the ADP-insensitive phosphoenzyme intermediate in the sarcoplasmic reticulum Ca2+-ATPase of which both Cys344 and Cys364 are modified by N-ethylmaleimide.
Topics: Adenosine Diphosphate; Animals; Calcium-Transporting ATPases; Cysteine; Dimethyl Sulfoxide; Enzyme Inhibitors; Ethylmaleimide; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Phosphates; Phosphoproteins; Rabbits; Sarcoplasmic Reticulum; Time Factors | 1999 |
Functional analysis of conserved cysteines in heparan sulfate N-deacetylase-N-sulfotransferases.
Topics: Alanine; Amidohydrolases; Amino Acid Substitution; Animals; Catalysis; Cysteine; Dithiothreitol; Enzyme Inhibitors; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Liver; Rats; Recombinant Proteins; Sulfotransferases | 1999 |
Fluctuation of the first loop facing the matrix of the mitochondrial ADP/ATP carrier deduced from intermolecular cross-linking of Cys56 residues by bifunctional dimaleimides.
Topics: Adenosine Diphosphate; Animals; Biological Transport; Cattle; Cross-Linking Reagents; Cysteine; Ethylmaleimide; Glutathione; Maleimides; Mitochondria, Heart; Mitochondrial ADP, ATP Translocases; Protein Conformation; Solutions; Submitochondrial Particles | 1999 |
Involvement of essential cysteine and histidine residues in the activity of isolated glutaminase from tumour cells.
Topics: Animals; Binding Sites; Carcinoma, Ehrlich Tumor; Cysteine; Diethyl Pyrocarbonate; Enzyme Inhibitors; Ethylmaleimide; Glutaminase; Histidine; Hydrogen-Ion Concentration; Intracellular Membranes | 1998 |
Probing the topography of HIV-1 nucleocapsid protein with the alkylating agent N-ethylmaleimide.
Topics: Alkylating Agents; Amino Acid Sequence; Capsid; Capsid Proteins; Cysteine; Ethylmaleimide; Fluorescence Polarization; gag Gene Products, Human Immunodeficiency Virus; Gene Products, gag; HIV-1; Hydrogen-Ion Concentration; Molecular Sequence Data; Oligonucleotides; Peptide Fragments; Protein Conformation; Sequence Analysis; Viral Proteins; Zinc Fingers | 1998 |
An unconventional role for cytoplasmic disulfide bonds in vaccinia virus proteins.
Topics: Antiviral Agents; Cysteine; Cytoplasm; Cytosol; Dimerization; Disulfides; Dithiothreitol; Ethylmaleimide; HeLa Cells; Humans; Kinetics; Sulfhydryl Reagents; Tritium; Vaccinia virus; Viral Core Proteins; Viral Envelope Proteins | 1999 |
Inactivation of the purified bovine mu opioid receptor by sulfhydryl reagents.
Topics: Animals; Benzomorphans; Cattle; Cell Membrane; Corpus Striatum; Cysteine; Dithionitrobenzoic Acid; Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; Enkephalins; Ethylmaleimide; Kinetics; Ligands; Naloxone; Radioligand Assay; Receptors, Opioid, mu; Sulfhydryl Reagents; Tritium | 1999 |
Modifications of Ca2+ mobilization and noradrenaline release by S-nitroso-cysteine in PC12 cells.
Topics: Adenosine Triphosphate; Animals; Biological Transport; Calcium; Cysteine; Dithiothreitol; Ethylmaleimide; Nitric Oxide Donors; Nitroso Compounds; Norepinephrine; PC12 Cells; Rats; Receptors, Purinergic; S-Nitrosothiols; Sulfhydryl Compounds | 1999 |
Identification of the amine-polyamine-choline transporter superfamily 'consensus amphipathic region' as the target for inactivation of the Escherichia coli GABA transporter GabP by thiol modification reagents. Role of Cys-300 in restoring thiol sensitivit
Topics: 4-Chloromercuribenzenesulfonate; Amines; Amino Acid Substitution; Biological Transport; Cell Membrane Permeability; Choline; Consensus Sequence; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Kinetics; Membrane Proteins; Membrane Transport Modulators; Membrane Transport Proteins; Mesylates; Models, Biological; Organic Anion Transporters; Polyamines; Protein Conformation; Substrate Specificity; Sulfhydryl Compounds; Sulfhydryl Reagents | 1999 |
A Cys-less variant of the bacterial ATP binding cassette protein MalK is functional in maltose transport and regulation.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; ATP-Binding Cassette Transporters; Bacterial Proteins; Biological Transport; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Maltose; Mutagenesis, Site-Directed; Salmonella typhimurium; Serine | 1999 |
Biochemical characterization of human S-nitrosohemoglobin. Effects on oxygen binding and transnitrosation.
Topics: Allosteric Regulation; Cysteine; Erythrocytes; Ethylmaleimide; Glutathione; Hemoglobins; Humans; Kinetics; Nitrosation; Nitroso Compounds; Oxygen; Penicillamine; S-Nitrosoglutathione; S-Nitrosothiols; Spectrophotometry; Sulfhydryl Compounds; Vasodilation | 1999 |
Targeted cross-linking and DNA cleavage within model chromatin complexes.
Topics: Amino Acid Substitution; Animals; Carbon Radioisotopes; Cattle; Chickens; Chromatin; Cloning, Molecular; Cross-Linking Reagents; Cysteine; DNA; DNA, Ribosomal; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Ethylmaleimide; Histones; Indicators and Reagents; Mutagenesis, Site-Directed; Plasmids; Polymerase Chain Reaction; Radioisotope Dilution Technique; Recombinant Proteins; RNA, Ribosomal, 5S; Xenopus | 1999 |
Scanning cysteine accessibility of EmrE, an H+-coupled multidrug transporter from Escherichia coli, reveals a hydrophobic pathway for solutes.
Topics: Acriflavine; Amino Acid Sequence; Antiporters; ATP Binding Cassette Transporter, Subfamily B, Member 1; Bacterial Proteins; Carrier Proteins; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethidium; Ethylmaleimide; Membrane Proteins; Molecular Sequence Data; Phenotype; Protein Structure, Secondary; Solutions | 1999 |
Glutamyl substrate-induced exposure of a free cysteine residue in the vitamin K-dependent gamma-glutamyl carboxylase is critical for vitamin K epoxidation.
Topics: Amino Acid Sequence; Animals; Binding Sites; Carbon-Carbon Ligases; Cysteine; Enzyme Activation; Epoxy Compounds; Ethylmaleimide; Glutamic Acid; Humans; Molecular Sequence Data; Oligopeptides; Peptides; Protein Precursors; Substrate Specificity; Sulfhydryl Reagents; Vitamin K | 1999 |
Chemical modification of rat hepatic microsomes with N-ethylmaleimide results in inactivation of both UDP-N-acetylglucosamine-dependent stimulation of glucuronidation and UDP-glucuronic acid uptake.
Topics: Animals; Cysteine; Dose-Response Relationship, Drug; Endoplasmic Reticulum; Ethylmaleimide; Glucuronosyltransferase; Kinetics; Male; Microsomes, Liver; Nitrophenols; Rats; Rats, Wistar; Testosterone; Uridine Diphosphate Glucuronic Acid | 1999 |
N-ethylmaleimide inhibits Ncd motor function by modification of a cysteine in the stalk domain.
Topics: Adenosine Triphosphatases; Amino Acid Sequence; Animals; Cysteine; Drosophila Proteins; Ethylmaleimide; Kinesins; Microtubules; Molecular Motor Proteins; Molecular Sequence Data; Plasmids; Protein Binding; Protein Structure, Tertiary | 1999 |
Effect of cysteine residues on the activity of arginyl-tRNA synthetase from Escherichia coli.
Topics: Acylation; Arginine-tRNA Ligase; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Escherichia coli; Ethylmaleimide; Gene Expression Regulation, Enzymologic; Iodoacetamide; Kinetics; Mutagenesis, Site-Directed; Recombinant Proteins; Sulfhydryl Reagents; Titrimetry | 1999 |
Role of conserved Arg40 and Arg117 in the Na+/proline transporter of Escherichia coli.
Topics: Amino Acid Sequence; Amino Acid Substitution; Amino Acid Transport Systems, Neutral; Arginine; Biological Transport, Active; Blotting, Western; Conserved Sequence; Cysteine; Diffusion; Escherichia coli; Ethylmaleimide; Kinetics; Membrane Transport Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Proline; Sodium | 1999 |
Cysteine-scanning mutagenesis around transmembrane segment VI of Tn10-encoded metal-tetracycline/H(+) antiporter.
Topics: Amino Acid Sequence; Cell Membrane; Chemical Phenomena; Chemistry, Physical; Cysteine; Escherichia coli; Ethylmaleimide; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Protein Structure, Secondary; Sulfhydryl Compounds; Tetracycline Resistance | 1999 |
A conserved serine-rich stretch in the glutamate transporter family forms a substrate-sensitive reentrant loop.
Topics: Amino Acid Motifs; Amino Acid Sequence; Amino Acid Transport System X-AG; ATP-Binding Cassette Transporters; Conserved Sequence; Cysteine; Ethylmaleimide; Glutamic Acid; Molecular Sequence Data; Serine | 1999 |
A role for cysteine 3635 of RYR1 in redox modulation and calmodulin binding.
Topics: Alkylation; Amino Acid Sequence; Animals; Calmodulin; Cysteine; Ethylmaleimide; Molecular Sequence Data; Oxidation-Reduction; Rabbits; Ryanodine Receptor Calcium Release Channel; Structure-Activity Relationship | 1999 |
Assignment of a single disulfide bridge in rat liver methionine adenosyltransferase.
Topics: Amino Acid Sequence; Animals; Cellulase; Cysteine; Dimerization; Disulfides; Dithiothreitol; Ethylmaleimide; Liver; Methionine Adenosyltransferase; Molecular Sequence Data; Nitric Oxide Donors; Peptide Fragments; Peptide Mapping; Protein Denaturation; Protein Structure, Quaternary; Rats; Sequence Analysis, Protein; Trypsin; Urea | 2000 |
Disulfide assignment of the C-terminal cysteine knot of agouti-related protein (AGRP) by direct sequencing analysis.
Topics: Agouti-Related Protein; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Ethylmaleimide; Humans; Intercellular Signaling Peptides and Proteins; Peptide Mapping; Proteins; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 1999 |
Cysteine 111 affects coupling of single-stranded DNA binding to ATP hydrolysis in the herpes simplex virus type-1 origin-binding protein.
Topics: Adenosine Triphosphate; Alanine; Amino Acid Sequence; Cysteine; Dithiothreitol; DNA Helicases; DNA Replication; DNA-Binding Proteins; DNA, Single-Stranded; Ethylmaleimide; Hydrolysis; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Sequence Homology, Amino Acid; Viral Proteins | 2000 |
Cysteine scanning mutagenesis of helices 2 and 7 in GLUT1 identifies an exofacial cleft in both transmembrane segments.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Computer Simulation; Cysteine; Ethylmaleimide; Glucose Transporter Type 1; Glutamine; Humans; Isoleucine; Membrane Proteins; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Sulfhydryl Reagents | 2000 |
Involvement of Hg2+-sensitive sulfhydryl groups in regulating noradrenaline release induced by S-nitrosocysteine in rat brain slices.
Topics: Animals; Brain; Calcium Chloride; Central Nervous System Stimulants; Cerebral Cortex; Cysteine; Diethylamines; Ethylmaleimide; In Vitro Techniques; Male; Mercuric Chloride; Mercury; Nitroso Compounds; Norepinephrine; p-Chloromercuribenzoic Acid; Rats; Rats, Wistar; S-Nitrosothiols; Sulfhydryl Compounds; Thiophenes | 2000 |
Cysteine-scanning mutagenesis around transmembrane segments 1 and 11 and their flanking loop regions of Tn10-encoded metal-Tetracycline/H+ antiporter.
Topics: Amino Acid Sequence; Antiporters; Bacterial Proteins; Carbon Radioisotopes; Cysteine; Disulfides; DNA Transposable Elements; Ethylmaleimide; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed | 2000 |
The noncatalytic beta-propeller domain of prolyl oligopeptidase enhances the catalytic capability of the peptidase domain.
Topics: Amino Acid Substitution; Animals; Brain; Catalysis; Catalytic Domain; Cloning, Molecular; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Mutagenesis, Site-Directed; Prolyl Oligopeptidases; Protein Structure, Secondary; Recombinant Proteins; Serine Endopeptidases; Substrate Specificity; Swine | 2000 |
The repression of nuclear factor I/CCAAT transcription factor (NFI/CTF) transactivating domain by oxidative stress is mediated by a critical cysteine (Cys-427).
Topics: Binding Sites; CCAAT-Enhancer-Binding Proteins; Cysteine; DNA-Binding Proteins; Dose-Response Relationship, Drug; Ethylmaleimide; Humans; Hydrogen Peroxide; NFI Transcription Factors; Nuclear Proteins; Oxidants; Oxidative Stress; Protein Conformation; Sulfhydryl Reagents; Transcription Factors; Transcriptional Activation; Tumor Cells, Cultured; Y-Box-Binding Protein 1 | 2000 |
Drug-stimulated ATPase activity of human P-glycoprotein is blocked by disulfide cross-linking between the nucleotide-binding sites.
Topics: Adenosine Triphosphatases; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Cell Line; Cell Membrane; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Humans; Models, Biological; Mutagenesis; Nucleotides; Oxygen; Protein Structure, Tertiary; Sulfhydryl Reagents; Verapamil | 2000 |
Inhibition of phospholipase A(2) activity by S-nitroso-cysteine in a cyclic GMP-independent manner in PC12 cells.
Topics: Animals; Arachidonic Acid; Cyclic GMP; Cysteine; Cytosol; Dose-Response Relationship, Drug; Enzyme Activation; Enzyme Inhibitors; Ethylmaleimide; Intercellular Signaling Peptides and Proteins; Nitroso Compounds; PC12 Cells; Peptides; Phospholipases A; Rats; S-Nitrosothiols; Tritium; Wasp Venoms | 2000 |
Close approximation of putative alpha -helices II, IV, VII, X, and XI in the translocation pathway of the lactose transport protein of Streptococcus thermophilus.
Topics: Amino Acid Sequence; Binding Sites; Biological Transport; Cysteine; Escherichia coli Proteins; Ethylmaleimide; Lactose; Ligands; Membrane Transport Proteins; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Protons; Sequence Homology, Amino Acid; Streptococcus; Suppression, Genetic; Symporters | 2000 |
The sucrose permease of Escherichia coli: functional significance of cysteine residues and properties of a cysteine-less transporter.
Topics: Alkylation; Biological Transport, Active; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Transport Modulators; Membrane Transport Proteins; Mutagenesis, Site-Directed; Serine; Sucrose | 2000 |
Functional conservation in the putative substrate binding site of the sucrose permease from Escherichia coli.
Topics: Alkylation; Arginine; Aspartic Acid; Binding Sites; Biological Transport, Active; Chromatography, Affinity; Conserved Sequence; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Iodoacetic Acid; Membrane Transport Proteins; Mutagenesis, Site-Directed; Serine; Substrate Specificity; Sucrose | 2000 |
Nitric oxide and thiol reagent modulation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeci.
Topics: Animals; Calcium; Cecum; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Glutathione; Guinea Pigs; Immunohistochemistry; In Vitro Techniques; Membrane Potentials; Muscle, Smooth; Nitric Oxide; Nitric Oxide Donors; Nitroprusside; Nitroso Compounds; Oxidation-Reduction; Potassium Channels; Pyridines; S-Nitrosothiols; Sulfhydryl Reagents; Thimerosal | 2000 |
Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking.
Topics: Amino Acid Sequence; Bacterial Proteins; Biological Transport; Cell Membrane; Cell Membrane Permeability; Cross-Linking Reagents; Cysteine; Dimerization; Disulfides; Escherichia coli Proteins; Ethylmaleimide; Membrane Proteins; Molecular Sequence Data; Mutation; Recombinant Proteins; SEC Translocation Channels; Stilbenes; Sulfonic Acids | 2000 |
The presence of an aqueous cavity in the proton-pumping pathway of the pyridine nucleotide transhydrogenase of Escherichia coli is suggested by the reaction of the enzyme with sulfhydryl inhibitors.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Cell Membrane; Cysteine; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Ethylmaleimide; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP Transhydrogenases; Plasmids; Protein Structure, Tertiary; Protons; Sulfhydryl Reagents; Time Factors; Water | 2000 |
Identification of a cysteine residue in the active site of nitroalkane oxidase by modification with N-ethylmaleimide.
Topics: Amino Acid Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cysteine; Dioxygenases; Ethylmaleimide; Flavin-Adenine Dinucleotide; Flavoproteins; Fusarium; Half-Life; Kinetics; Mass Spectrometry; Molecular Sequence Data; Oxygenases; Peptide Fragments; Peptide Mapping; Sequence Analysis, Protein; Trypsin; Valerates | 2000 |
The barley scutellar peptide transporter: biochemical characterization and localization to the plasma membrane.
Topics: Affinity Labels; Amino Acid Sequence; Animals; Carrier Proteins; Cell Membrane; Conserved Sequence; Cysteine; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Hordeum; Membrane Transport Proteins; Molecular Sequence Data; Molecular Weight; Oocytes; Plant Proteins; Seeds; Sequence Alignment; Xenopus laevis | 2000 |
Polymorphism of the glutathione transferase subunit 3 in Sprague-Dawley rats involves a reactive cysteine residue.
Topics: Animals; Chromatography, Agarose; Chromatography, High Pressure Liquid; Cloning, Molecular; Cysteine; Dithiothreitol; DNA, Complementary; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Glutathione; Glutathione Transferase; Hydrogen Peroxide; Hydrogen-Ion Concentration; Immunoblotting; Liver; Male; Oxidative Stress; Polymorphism, Genetic; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, Protein; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity; Time Factors; Trypsin; Xanthine; Xanthine Oxidase | 2000 |
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helix VII.
Topics: Amino Acid Sequence; Amino Acid Substitution; Cold Temperature; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Hot Temperature; Membrane Proteins; Membrane Transport Proteins; Mesylates; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protein Structure, Secondary; Sulfhydryl Reagents; Symporters | 2000 |
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: N-ethylmaleimide-sensitive face of helix II.
Topics: Alanine; Amino Acid Sequence; Cold Temperature; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Glycine; Hot Temperature; Leucine; Membrane Proteins; Membrane Transport Modulators; Membrane Transport Proteins; Mesylates; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Periplasm; Protein Structure, Secondary; Solvents; Sulfhydryl Reagents; Symporters | 2000 |
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helix X.
Topics: Amino Acid Sequence; Amino Acid Substitution; Ascorbic Acid; Cold Temperature; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Hot Temperature; Ligands; Membrane Proteins; Membrane Transport Proteins; Mesylates; Methylphenazonium Methosulfate; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protein Structure, Secondary; Sulfhydryl Reagents; Symporters | 2000 |
Mouse T-cell antigen rt6.1 has thiol-dependent NAD glycohydrolase activity.
Topics: Adenosine Diphosphate Ribose; ADP Ribose Transferases; Animals; Antigens, Differentiation, T-Lymphocyte; COS Cells; Cysteine; Dithiothreitol; Escherichia coli; Ethylmaleimide; Histocompatibility Antigens; Hydrolysis; Kinetics; Macrophages, Peritoneal; Membrane Glycoproteins; Mice; Mice, Inbred BALB C; Mutation; NAD; NAD+ Nucleosidase; Poly(ADP-ribose) Polymerases; Recombinant Fusion Proteins; Sulfhydryl Compounds; T-Lymphocytes; Transfection | 2000 |
Modification of cysteine residues by N-ethylmaleimide inhibits annexin II tetramer mediated liposome aggregation.
Topics: Animals; Annexin A2; Calcium; Cattle; Cysteine; Ethylmaleimide; In Vitro Techniques; Liposomes; Membrane Fusion; Protein Conformation; Protein Structure, Quaternary; Sulfhydryl Reagents | 2000 |
The role of cysteine residues in tellurite resistance mediated by the TehAB determinant.
Topics: Alanine; Alkylating Agents; Bacterial Proteins; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Drug Resistance; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Iodoacetamide; Iodoacetates; Ligands; Microbial Sensitivity Tests; Mutagenesis, Site-Directed; Phenotype; Plasmids; Protein Binding; Protein Conformation; Protein Denaturation; S-Adenosylmethionine; Sulfhydryl Reagents; Tellurium; Up-Regulation | 2000 |
Effects of nitric oxide donors on Ca2+-dependent [14C]GABA release from brain synaptosomes: the role of SH-groups.
Topics: Animals; Brain; Calcium; Carbon Isotopes; Cyclic GMP; Cysteine; Dibutyryl Cyclic GMP; Dose-Response Relationship, Drug; Enzyme Activation; Enzyme Inhibitors; Ethylmaleimide; gamma-Aminobutyric Acid; Guanylate Cyclase; Hydroxylamine; Kinetics; Male; Methylene Blue; Nitric Oxide Donors; Nitroprusside; Nitroso Compounds; Potassium; Rats; Rats, Wistar; S-Nitrosothiols; src Homology Domains; Sulfhydryl Reagents; Synaptosomes; Time Factors | 2000 |
Modification of cysteine residues in the ChlI and ChlH subunits of magnesium chelatase results in enzyme inactivation.
Topics: Adenosine Triphosphatases; Bacterial Proteins; Cysteine; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Lyases | 2000 |
A histidine residue in the catalytic mechanism distinguishes Vibrio harveyi aldehyde dehydrogenase from other members of the aldehyde dehydrogenase superfamily.
Topics: Acyl Coenzyme A; Acylation; Acyltransferases; Aldehyde Dehydrogenase; Animals; Binding Sites; Catalytic Domain; Cysteine; Enzyme Activation; Esterases; Ethylmaleimide; Histidine; Kinetics; Multigene Family; Mutagenesis, Site-Directed; NADP; Niacinamide; Rats; Sequence Homology, Amino Acid; Thiolester Hydrolases; Vibrio | 2000 |
Identification of the vitamin K-dependent carboxylase active site: Cys-99 and Cys-450 are required for both epoxidation and carboxylation.
Topics: Amino Acid Sequence; Amino Acid Substitution; Binding Sites; Carbon Radioisotopes; Carbon-Carbon Ligases; Cysteine; Epoxide Hydrolases; Ethylmaleimide; Humans; Kinetics; Peptide Fragments; Peptide Mapping; Recombinant Proteins; Spectrometry, Mass, Electrospray Ionization; Trypsin | 2000 |
Cysteine residues in the D-galactose-H+ symport protein of Escherichia coli: effects of mutagenesis on transport, reaction with N-ethylmaleimide and antibiotic binding.
Topics: Amino Acid Sequence; Anti-Bacterial Agents; Base Sequence; Biological Transport; Calcium-Binding Proteins; Colforsin; Cysteine; Cytochalasin B; DNA Primers; Eosine Yellowish-(YS); Ethylmaleimide; Galactose; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Periplasmic Binding Proteins; Protein Binding | 2001 |
Investigation of sulfhydryl groups in cabbage phospholipase D by combination of derivatization methods and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Topics: Brassica; Cysteine; DNA, Complementary; Ethylmaleimide; Iodoacetic Acid; p-Chloromercuribenzoic Acid; Phospholipase D; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin | 2001 |
Complete cysteine-scanning mutagenesis and site-directed chemical modification of the Tn10-encoded metal-tetracycline/H+ antiporter.
Topics: Amino Acid Sequence; Antiporters; Bacterial Proteins; Cysteine; DNA Transposable Elements; Ethylmaleimide; Molecular Sequence Data; Mutagenesis, Site-Directed | 2001 |
Enhanced potency of human Sonic hedgehog by hydrophobic modification.
Topics: Acyl Coenzyme A; Amides; Amino Acid Substitution; Animals; Cell Line; Circular Dichroism; Cysteine; Ethylmaleimide; Fatty Acids; Formaldehyde; Hedgehog Proteins; Humans; Indicators and Reagents; Intracellular Signaling Peptides and Proteins; Iodoacetamide; Membrane Proteins; Mice; Mice, Inbred C3H; Patched Receptors; Peptide Fragments; Protein Binding; Protein Processing, Post-Translational; Proteins; Receptors, Cell Surface; Signal Transduction; Spectrometry, Mass, Electrospray Ionization; Sulfhydryl Compounds; Thiazoles; Thiazolidines; Trans-Activators; Up-Regulation | 2001 |
Disulfide bond structure of the AVR9 elicitor of the fungal tomato pathogen Cladosporium fulvum: evidence for a cystine knot.
Topics: Alkylation; Amino Acid Sequence; Carboxypeptidases; Carboxypeptidases A; Cladosporium; Cysteine; Cystine; Disulfides; Ethylmaleimide; Fungal Proteins; Iodoacetamide; Molecular Sequence Data; Necrosis; Phosphines; Plant Diseases; Plant Leaves; Reducing Agents; Solanum lycopersicum; Sulfhydryl Compounds; Virulence | 2001 |
Mapping of contact sites in complex formation between transducin and light-activated rhodopsin by covalent crosslinking: use of a photoactivatable reagent.
Topics: Amino Acid Sequence; Animals; Azides; Binding Sites; Cattle; COS Cells; Cross-Linking Reagents; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Guanosine Diphosphate; Light; Lysine; Maleimides; Models, Molecular; Molecular Sequence Data; Mutation; Photolysis; Protein Binding; Protein Structure, Secondary; Pyridines; Rhodopsin; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transducin; Trypsin; Ultraviolet Rays | 2001 |
Conformation and polarity of the active site of xylanase I from Thermomonospora sp. as deduced by fluorescent chemoaffinity labeling. Site and significance of a histidine residue.
Topics: Actinomycetales; Affinity Labels; Amino Acids; Binding Sites; Circular Dichroism; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Enzyme Activation; Ethylmaleimide; Histidine; Hydroxymercuribenzoates; Indicators and Reagents; Kinetics; Lysine; Models, Chemical; o-Phthalaldehyde; Protease Inhibitors; Protein Binding; Protein Conformation; Sequence Analysis, Protein; Spectrometry, Fluorescence; Sulfhydryl Reagents; Time Factors; Trinitrobenzenesulfonic Acid; Urea; Xylan Endo-1,3-beta-Xylosidase; Xylosidases | 2001 |
Peroxynitrite-dependent activation of src tyrosine kinases lyn and hck in erythrocytes is under mechanistically different pathways of redox control.
Topics: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Alkylation; Cysteine; Diffusion; Dithiothreitol; Enzyme Activation; Erythrocytes; Ethylmaleimide; Glucose; Humans; Hydrogen Peroxide; Immunochemistry; Nitrates; Oxidation-Reduction; Protein Tyrosine Phosphatases; Protein-Tyrosine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-hck; Sequence Homology; Signal Transduction; Sodium Bicarbonate; src-Family Kinases; Substrate Specificity | 2001 |
Modifications of cysteine residues in the solution and membrane-associated conformations of phosphatidylinositol transfer protein have differential effects on lipid transfer activity.
Topics: Animals; Biological Transport, Active; Carrier Proteins; Colorimetry; Cysteine; Disulfides; Dithionitrobenzoic Acid; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Membrane Proteins; Mercaptoethanol; Mutagenesis, Site-Directed; Phosphatidylinositols; Phospholipid Transfer Proteins; Phospholipids; Protein Conformation; Protein Denaturation; Pyridines; Rats; Solutions; Sulfhydryl Compounds | 2001 |
The cysteine residue of the SoxY protein as the active site of protein-bound sulfur oxidation of Paracoccus pantotrophus GB17.
Topics: Bacterial Proteins; Catalytic Domain; Cysteine; Ethylmaleimide; Kinetics; Molecular Weight; Oxidation-Reduction; Oxidoreductases; Paracoccus; Protein Binding; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Sulfur | 2001 |
beta-Lactoglobulin A with N-ethylmaleimide-modified sulfhydryl residue, polymerized through intermolecular disulfide bridge on heating in the presence of dithiothreitol.
Topics: Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Hot Temperature; Lactoglobulins; Polymers; Sodium Chloride; Sulfhydryl Compounds | 2001 |
Targeted disulfide cross-linking of the MotB protein of Escherichia coli: evidence for two H(+) channels in the stator Complex.
Topics: Aspartic Acid; Bacterial Proteins; Cell Membrane; Cross-Linking Reagents; Cysteine; Dimerization; Disulfides; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethylmaleimide; Hydrogen; Immunoblotting; Iodine; Ions; Mesylates; Models, Biological; Mutation; Oxygen; Plasmids; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Protons | 2001 |
Two cysteine residues in the DNA-binding domain of CREB control binding to CRE and CREB-mediated gene expression.
Topics: Amino Acid Sequence; Cyclic AMP Response Element-Binding Protein; Cysteine; Deoxyribonuclease I; DNA Footprinting; Ethylmaleimide; Gene Expression Regulation; Gene Products, tax; Genes, Reporter; Humans; Isoenzymes; L-Lactate Dehydrogenase; Lactate Dehydrogenase 5; Models, Molecular; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Response Elements; RNA, Messenger; Transcriptional Activation; Tumor Cells, Cultured | 2001 |
Characterization of the binding of Photobacterium phosphoreum P-flavin by Vibrio harveyi Luciferase.
Topics: Binding Sites; Binding, Competitive; Chromatography, Gel; Cysteine; Ethylmaleimide; Flavins; Kinetics; Luciferases; Photobacterium; Protein Binding; Spectrometry, Fluorescence; Time Factors; Vibrio | 2001 |
The mitochondrial oxoglutarate carrier: cysteine-scanning mutagenesis of transmembrane domain IV and sensitivity of Cys mutants to sulfhydryl reagents.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Biological Transport, Active; Carrier Proteins; Cattle; Cysteine; Ethyl Methanesulfonate; Ethylmaleimide; Ketoglutaric Acids; Membrane Proteins; Membrane Transport Proteins; Mesylates; Mitochondria, Heart; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Secondary; Protein Structure, Tertiary; Proteolipids; Recombinant Proteins; Sulfhydryl Reagents | 2001 |
Membrane topology of a multidrug efflux transporter, AcrB, in Escherichia coli.
Topics: Amino Acid Sequence; Bacterial Proteins; Binding, Competitive; Carbon Radioisotopes; Carrier Proteins; Cloning, Molecular; Cysteine; Cytoplasm; Drug Resistance, Multiple; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Proteins; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Periplasm; Protein Conformation; Radioligand Assay; Stilbenes; Sulfonic Acids | 2002 |
UV resonance Raman study of beta93-modified hemoglobin A: chemical modifier-specific effects and added influences of attached poly(ethylene glycol) chains.
Topics: Binding Sites; Cysteine; Ethylmaleimide; Hemoglobin A; Humans; Ligands; Polyethylene Glycols; Protein Conformation; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman; Sulfhydryl Reagents; Surface-Active Agents | 2002 |
Trans membrane domain IV is involved in ion transport activity and pH regulation of the NhaA-Na(+)/H(+) antiporter of Escherichia coli.
Topics: Amino Acid Sequence; Cell Membrane; Cysteine; Cytoplasm; Dose-Response Relationship, Drug; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Hydrogen-Ion Concentration; Ions; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Phenotype; Plasmids; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Transport; Sequence Homology, Amino Acid; Sodium; Sodium-Hydrogen Exchangers; Time Factors | 2002 |
The streptococcal hyaluronan synthases are inhibited by sulfhydryl-modifying reagents, but conserved cysteine residues are not essential for enzyme function.
Topics: Alanine; Amino Acid Sequence; Binding Sites; Carbohydrates; Cysteine; Dose-Response Relationship, Drug; Escherichia coli; Ethylmaleimide; Genetic Variation; Genetic Vectors; Glucuronosyltransferase; Glycosyltransferases; Hyaluronan Synthases; Kinetics; Mass Spectrometry; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Sequence Homology, Amino Acid; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptococcus; Structure-Activity Relationship; Sulfhydryl Reagents; Transferases; Xenopus Proteins | 2002 |
Site-directed mutation of conserved cysteine residues does not inactivate the Streptococcus pyogenes hyaluronan synthase.
Topics: Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution; Catalysis; Cell Membrane; Conserved Sequence; Cysteine; Disulfides; Enzyme Inhibitors; Escherichia coli; Ethylmaleimide; Glucuronosyltransferase; Glycosyltransferases; Hyaluronan Synthases; Kinetics; Membrane Proteins; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptococcus pyogenes; Structure-Activity Relationship; Transferases; Xenopus Proteins | 2001 |
Redox regulation of cytosolic glycerol-3-phosphate dehydrogenase: Cys(102) is the target of the redox control and essential for the catalytic activity.
Topics: Animals; Binding Sites; Catalysis; CHO Cells; Cricetinae; Cysteine; Cystine; Cytosol; Ethylmaleimide; Glutathione; Glycerolphosphate Dehydrogenase; In Vitro Techniques; Nitric Oxide Donors; Oxidation-Reduction; Penicillamine; Sodium Selenite; Transfection | 2002 |
Extracellular cysteines define ectopeptidase (APN, CD13) expression and function.
Topics: Amidohydrolases; Antigens, Surface; CD13 Antigens; Cysteine; DNA Primers; Ethylmaleimide; Green Fluorescent Proteins; Hexosaminidases; Humans; Immunoblotting; Iodoacetamide; Luminescent Proteins; Microscopy, Fluorescence; Mutagenesis, Site-Directed; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; Polymerase Chain Reaction; Protein Disulfide-Isomerases; Recombinant Fusion Proteins; Subcellular Fractions; U937 Cells | 2002 |
The essential role of a free sulfhydryl group in blocking the cholesteryl site of cholesteryl ester transfer protein (CETP).
Topics: 2-Naphthylamine; Amino Acid Sequence; Anilino Naphthalenesulfonates; Binding Sites; Biological Transport, Active; Carrier Proteins; Cholesterol Ester Transfer Proteins; Cholesterol Esters; Cysteine; Ethylmaleimide; Glycoproteins; In Vitro Techniques; Kinetics; Molecular Sequence Data; Peptide Fragments; Recombinant Proteins; Spectrometry, Fluorescence; Sulfhydryl Compounds | 2002 |
Evaluation of critical structural elements of UDP-sugar substrates and certain cysteine residues of a vertebrate hyaluronan synthase.
Topics: Animals; Binding Sites; Catalysis; Cysteine; Dose-Response Relationship, Drug; Enzyme Inhibitors; Ethylmaleimide; Glucuronosyltransferase; Glycosyltransferases; Hyaluronan Synthases; Kinetics; Membrane Proteins; Mutation; Polysaccharides; Protein Binding; Recombinant Proteins; Substrate Specificity; Sulfhydryl Reagents; Transferases; Uridine Diphosphate Sugars; Xenopus laevis; Xenopus Proteins | 2002 |
Site-directed mutagenesis of cysteine to serine in the DNA binding region of Nrf2 decreases its capacity to upregulate antioxidant response element-mediated expression and antioxidant induction of NAD(P)H:quinone oxidoreductase1 gene.
Topics: Amino Acid Sequence; Animals; Antioxidants; Cysteine; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Ethylmaleimide; Gene Expression Regulation; Humans; MafG Transcription Factor; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; NADH, NADPH Oxidoreductases; NF-E2-Related Factor 2; Repressor Proteins; Response Elements; Sequence Homology, Amino Acid; Serine; Structure-Activity Relationship; Trans-Activators; Tumor Cells, Cultured | 2002 |
Superoxide modification and inactivation of a neuronal receptor-like complex.
Topics: Animals; Cysteine; Ethylmaleimide; Glutamic Acid; Membrane Proteins; N-Methylaspartate; Point Mutation; Protein Binding; Rats; Reactive Oxygen Species; Receptors, N-Methyl-D-Aspartate; Superoxides; Synaptic Membranes; Tritium; Xanthine; Xanthine Oxidase | 2002 |
S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain.
Topics: Binding Sites; Brain; Calbindins; Calcium; Cysteine; Ethylmaleimide; Humans; In Vitro Techniques; Nitric Oxide; Nitric Oxide Donors; Recombinant Proteins; S-Nitrosoglutathione; S100 Calcium Binding Protein G; Spectrometry, Fluorescence; Spectrometry, Mass, Electrospray Ionization; Sulfhydryl Reagents | 2002 |
Changing the lactose permease of Escherichia coli into a galactose-specific symporter.
Topics: Alanine; Calcium-Binding Proteins; Cysteine; Disaccharides; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Galactose; Lactose; Membrane Transport Proteins; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Periplasmic Binding Proteins; Substrate Specificity; Symporters | 2002 |
A possible effect of sulfhydryl reagents on the contractile activity of the rat detrusor muscle.
Topics: Acetylcholine; Animals; Cysteine; Diuretics; Dose-Response Relationship, Drug; Electric Stimulation; Ethacrynic Acid; Ethylmaleimide; Glutathione; In Vitro Techniques; Muscle Contraction; Rats; Rats, Inbred WKY; Sulfhydryl Reagents; Urinary Bladder; Vasodilator Agents; Verapamil | 2002 |
Site-directed mutagenesis and chemical modification of the six native cysteine residues of the rat mitochondrial carnitine carrier: implications for the role of cysteine-136.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Carrier Proteins; Cloning, Molecular; Cysteine; Escherichia coli; Ethyl Methanesulfonate; Ethylmaleimide; Mesylates; Mitochondria; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Organic Cation Transport Proteins; Protein Structure, Secondary; Rats; Recombinant Proteins; Solute Carrier Family 22 Member 5; Sulfhydryl Reagents | 2002 |
The roles of thiols in the bacterial organomercurial lyase (MerB).
Topics: Amino Acid Sequence; Bacterial Proteins; Cysteine; Cytosol; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Hydroxymercuribenzoates; Iodoacetamide; Kinetics; Lyases; Molecular Sequence Data; Mutagenesis, Site-Directed; Organomercury Compounds; Sequence Homology, Amino Acid; Substrate Specificity; Sulfhydryl Compounds | 2002 |
Identification of cysteines involved in ligand binding to the human melatonin MT(2) receptor.
Topics: Alkylating Agents; Cysteine; Dithiothreitol; DNA, Complementary; Epitopes; Ethylmaleimide; Humans; Immunohistochemistry; Ligands; Melatonin; Microscopy, Confocal; Mutagenesis, Site-Directed; Oligopeptides; Peptides; Receptors, Cell Surface; Receptors, Cytoplasmic and Nuclear; Receptors, Melatonin; Sulfhydryl Compounds; Transfection | 2002 |
Thiamine transport in Escherichia coli: the mechanism of inhibition by the sulfhydryl-specific modifier N-ethylmaleimide.
Topics: Adenosine Triphosphate; Amino Acid Sequence; ATP-Binding Cassette Transporters; Binding Sites; Biological Transport; Cysteine; Escherichia coli; Ethylmaleimide; Kinetics; Membrane Transport Modulators; Membrane Transport Proteins; Molecular Sequence Data; Thiamine | 2002 |
The transmembrane domains of the ABC multidrug transporter LmrA form a cytoplasmic exposed, aqueous chamber within the membrane.
Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins; Biological Transport; Cell Membrane; Cysteine; Drug Resistance, Multiple; Ethylmaleimide; Fluoresceins; Fluorescent Dyes; Lactococcus lactis; Models, Molecular; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Plasmids; Protein Conformation; Protein Structure, Secondary; Recombinant Proteins; Sequence Deletion; Solvents | 2002 |
Inactivation of annexin II tetramer by S-nitrosoglutathione.
Topics: Animals; Annexin A2; Calcium; Cattle; Chromaffin Granules; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Ethylmaleimide; Glutathione; In Vitro Techniques; Liposomes; Lung; Membrane Fusion; Membranes, Artificial; Nitrates; Nitric Oxide Donors; Nitrites; Nitroprusside; Protein Conformation; S-Nitrosoglutathione | 2002 |
SecY-SecY and SecY-SecG contacts revealed by site-specific crosslinking.
Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Cross-Linking Reagents; Cysteine; Cytoplasm; Disulfides; Escherichia coli Proteins; Ethylmaleimide; Fluoresceins; Membrane Proteins; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Precursors; Protein Structure, Tertiary; Protein Transport; SEC Translocation Channels | 2002 |
Conserved residues in the putative catalytic triad of human bile acid Coenzyme A:amino acid N-acyltransferase.
Topics: Acyl Coenzyme A; Acyltransferases; Amino Acid Sequence; Aspartic Acid; Bile Acids and Salts; Catalysis; Catalytic Domain; Cholic Acids; Coenzyme A; Conserved Sequence; Cysteine; DNA, Complementary; Dose-Response Relationship, Drug; Ethylmaleimide; Histidine; Humans; Kinetics; Models, Chemical; Molecular Sequence Data; Mutation; Protein Binding; Sequence Homology, Amino Acid; Spectrometry, Mass, Electrospray Ionization; Time Factors; Trypsin | 2002 |
Site-specific modification of single cysteine Pax3 mutants reveals reciprocal regulation of DNA binding activity of the paired and homeo domain.
Topics: Amino Acid Sequence; Animals; Binding Sites; Bridged Bicyclo Compounds; COS Cells; Cysteine; DNA; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Homeodomain Proteins; Immunoblotting; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Paired Box Transcription Factors; PAX3 Transcription Factor; Protein Binding; Protein Structure, Tertiary; Transcription Factors | 2002 |
Identification of S-glutathionylated cellular proteins during oxidative stress and constitutive metabolism by affinity purification and proteomic analysis.
Topics: Cell Line; Cells, Cultured; Chromatography, Affinity; Cysteine; Electrophoresis, Gel, Two-Dimensional; Endothelium, Vascular; Ethylmaleimide; Glutaredoxins; Glutathione; Humans; Oxidation-Reduction; Oxidative Stress; Oxidoreductases; Polymerase Chain Reaction; Proteins; Proteome; Umbilical Veins | 2002 |
Activation of glucuronidation through reduction of a disulfide bond in rat UDP-glucuronosyltransferase 1A6.
Topics: Animals; Biotransformation; COS Cells; Cysteine; Disulfides; Dithiothreitol; Enzyme Inhibitors; Ethylmaleimide; Glucuronides; Glucuronosyltransferase; Isoenzymes; Male; Maleimides; Microsomes, Liver; Nitrophenols; Oxidation-Reduction; Rats; Rats, Gunn; Rats, Wistar; Reducing Agents | 2002 |
Cd2+ and the N-terminal metal-binding domain protect the putative membranous CPC motif of the Cd2+-ATPase of Listeria monocytogenes.
Topics: Adenosine Triphosphatases; Amino Acid Motifs; Biological Transport, Active; Cadmium; Cell Membrane; Cysteine; Ethylmaleimide; Listeria monocytogenes; Phosphorylation; Protein Structure, Tertiary; Sulfhydryl Reagents | 2003 |
Complete disulfide bond assignment of a recombinant immunoglobulin G4 monoclonal antibody.
Topics: Amino Acid Sequence; Animals; Antibodies, Monoclonal; CHO Cells; Chromatography, High Pressure Liquid; Cricetinae; Cysteine; Disulfides; Ethylmaleimide; Guanidine; Humans; Immunoglobulin G; Metalloendopeptidases; Molecular Sequence Data; Peptides; Recombinant Proteins; Sequence Analysis, Protein; Spectrometry, Mass, Electrospray Ionization | 2002 |
Aqueous access channels in subunit a of rotary ATP synthase.
Topics: Adenosine Triphosphate; Amino Acid Substitution; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Glucose; Ion Channels; Kinetics; Mitochondrial Proton-Translocating ATPases; Mutagenesis, Site-Directed; NAD; Plasmids; Protein Structure, Secondary; Protein Subunits; Quinacrine; Recombinant Proteins; Spectrometry, Fluorescence; Succinates | 2003 |
Conserved residues R420 and Q428 in a cytoplasmic loop of the citrate/malate transporter CimH of Bacillus subtilis are accessible from the external face of the membrane.
Topics: Arginine; Bacillus subtilis; Bacterial Proteins; Calcium; Carrier Proteins; Cell Membrane; Cell Membrane Permeability; Citric Acid; Conserved Sequence; Cysteine; Cytoplasm; Ethyl Methanesulfonate; Ethylmaleimide; Glutamine; Hydroxy Acids; Lysine; Malates; Mutagenesis, Site-Directed; Organic Anion Transporters; Plasmids; Substrate Specificity | 2003 |
Coupling of contact sensitizers to thiol groups is a key event for the activation of monocytes and monocyte-derived dendritic cells.
Topics: Acetates; Anti-Infective Agents; Antioxidants; Cysteine; Dendritic Cells; Ethylmaleimide; Humans; Interleukin-1; Lysine; Maleimides; MAP Kinase Signaling System; Monocytes; Phosphorylation; Picryl Chloride; Succinimides; Sulfhydryl Compounds; Sulfhydryl Reagents; Thiazoles; Tyrosine | 2003 |
Human sensitive to apoptosis gene protein inhibits peroxynitrite-induced DNA damage.
Topics: 8-Hydroxy-2'-Deoxyguanosine; Antioxidants; Cysteine; Deoxyguanosine; DNA Damage; Dose-Response Relationship, Drug; Ethylmaleimide; Humans; Molsidomine; Peroxynitrous Acid; RNA-Binding Proteins; Sulfhydryl Reagents; Ubiquitin-Protein Ligases | 2003 |
Stable monomeric intermediate with exposed Cys-119 is formed during heat denaturation of beta-lactoglobulin.
Topics: Animals; Chromatography; Cysteine; Disulfides; Ethylmaleimide; Hot Temperature; Lactoglobulins; Protein Conformation; Protein Denaturation; Protein Folding; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 2003 |
Aromatic stacking in the sugar binding site of the lactose permease.
Topics: Alkylation; Amino Acids, Aromatic; Binding Sites; Biological Transport, Active; Cysteine; Escherichia coli Proteins; Ethylmaleimide; Kinetics; Lactose; Membrane Transport Proteins; Monosaccharide Transport Proteins; Nitrophenylgalactosides; Phenylalanine; Substrate Specificity; Symporters; Thiogalactosides; Tryptophan; Tyrosine | 2003 |
Mapping out regions on the surface of the aspartate receptor that are essential for kinase activation.
Topics: Aspartic Acid; Chemotaxis; Cysteine; Cytoplasm; Dimerization; Enzyme Activation; Ethylmaleimide; Fluoresceins; Fluorescent Dyes; Models, Molecular; Mutagenesis, Site-Directed; Peptide Library; Protein Interaction Mapping; Protein Kinases; Protein Structure, Tertiary; Receptors, Amino Acid; Rhodamines; Salmonella typhimurium; Surface Properties | 2003 |
Inhibition of Streptomyces chromofuscus phospholipase D activity by dichloro-(2,2':6',2''-terpyridine)-platinum (II) dihydrate.
Topics: Aluminum Compounds; Amino Acid Motifs; Brassica; Calcium; Conserved Sequence; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Fluorides; Histidine; Hydrolysis; Isoenzymes; Phospholipase D; Platinum Compounds; Streptomyces; Structure-Activity Relationship | 2002 |
Binding of fatty acids facilitates oxidation of cysteine-34 and converts copper-albumin complexes from antioxidants to prooxidants.
Topics: Antioxidants; Ascorbic Acid; Copper; Cysteine; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Fatty Acids; Humans; In Vitro Techniques; Macromolecular Substances; Nitric Oxide; Oxidants; Oxidation-Reduction; Protein Conformation; Serum Albumin | 2003 |
Roles of individual amino acids in helix 14 of the membrane domain of proton-translocating transhydrogenase from Escherichia coli as deduced from cysteine mutagenesis.
Topics: Alkaline Phosphatase; Amino Acids; Binding Sites; Cysteine; Escherichia coli; Ethylmaleimide; Glycine; Models, Chemical; Mutagenesis, Site-Directed; Mutation; NAD; NADP; NADP Transhydrogenases; Plasmids; Protein Structure, Tertiary; Protons; Serine | 2003 |
Structural comparison of human monoamine oxidases A and B: mass spectrometry monitoring of cysteine reactivities.
Topics: Amino Acid Sequence; Animals; Biotinylation; Clorgyline; Cysteine; Disulfides; Enzyme Stability; Ethylmaleimide; Gene Expression; Humans; Kinetics; Mitochondria; Molecular Sequence Data; Monoamine Oxidase; Monoamine Oxidase Inhibitors; Pargyline; Pichia; Recombinant Proteins; Sequence Alignment; Spectrometry, Mass, Electrospray Ionization; Structure-Activity Relationship | 2003 |
ATP binding to the first nucleotide binding domain of multidrug resistance-associated protein plays a regulatory role at low nucleotide concentration, whereas ATP hydrolysis at the second plays a dominant role in ATP-dependent leukotriene C4 transport.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Amino Acid Substitution; Animals; Binding Sites; Biological Transport; Cell Line; Cricetinae; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Humans; Hydrolysis; Kidney; Leukotriene C4; Mutagenesis; Protein Structure, Tertiary | 2003 |
Accessibility of cysteine residues in a cytoplasmic loop of CitS of Klebsiella pneumoniae is controlled by the catalytic state of the transporter.
Topics: Bacterial Proteins; Binding Sites; Carbon Isotopes; Carrier Proteins; Catalysis; Cell Membrane; Cell Membrane Permeability; Citric Acid; Cysteine; Cytoplasm; Ethylmaleimide; Klebsiella pneumoniae; Maleimides; Mesylates; Proton-Motive Force; Sodium; Transport Vesicles | 2003 |
Transmembrane domain II of the Na+/proline transporter PutP of Escherichia coli forms part of a conformationally flexible, cytoplasmic exposed aqueous cavity within the membrane.
Topics: Amino Acid Sequence; Amino Acid Transport Systems, Neutral; Biological Transport; Cell Membrane; Cysteine; Cytoplasm; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethylmaleimide; Fluoresceins; Ligands; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Plasmids; Proline; Protein Conformation; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Sodium | 2003 |
Disulfide mapping of the cyclotide kalata B1. Chemical proof of the cystic cystine knot motif.
Topics: Amino Acid Motifs; Amino Acid Sequence; Chromatography, High Pressure Liquid; Conserved Sequence; Cyclotides; Cysteine; Disulfides; Ethylmaleimide; Hydrogen-Ion Concentration; Ions; Mass Spectrometry; Molecular Sequence Data; Oldenlandia; Peptides, Cyclic; Protein Folding; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin | 2003 |
Purification and characterization of the N-terminal nucleotide binding domain of an ABC drug transporter of Candida albicans: uncommon cysteine 193 of Walker A is critical for ATP hydrolysis.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Motifs; Amino Acid Sequence; ATP-Binding Cassette Transporters; Base Sequence; Candida albicans; Cysteine; Ethylmaleimide; Fungal Proteins; Hydrolysis; Membrane Transport Proteins; Molecular Sequence Data; Nucleotides; Oligonucleotides; Plasmids; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Homology, Amino Acid; Spectrometry, Fluorescence | 2003 |
[Use of N-ethylmaleimide in the chromatography of amino acid mixtures containing cysteine].
Topics: Amino Acids; Chromatography; Cysteine; Ethylmaleimide | 1953 |
A RAPID ASSAY FOR SULPHITE REDUCTASE.
Topics: Biological Assay; Colorimetry; Culture Media; Cysteine; Escherichia coli; Ethylmaleimide; Glutathione; Metabolism; Methionine; NADP; Oxidoreductases; Oxidoreductases Acting on Sulfur Group Donors; Pyrroles; Research; Sulfides; Sulfites; Sulfonic Acids; Thiosulfates | 1964 |
AN INTERMEDIATE TRAPPED BY MALEIMIDES IN A PYRIDOXAL-PHOSPHATE POTENTIATED ENZYMATIC ELIMINATION REACTION.
Topics: Amino Acids; Butyrates; Carbon Isotopes; Chromatography; Cysteine; Ethylmaleimide; Homocysteine; Keto Acids; Ligases; Lyases; Maleimides; Neurospora; Phosphates; Pyridoxal Phosphate; Pyrroles; Pyruvates; Research; Threonine | 1964 |
PARTIAL PURIFICATION AND SOME PROPERTIES OF A PROTEINASE FROM ALBACORE (GERMO ALALUNGA) MUSCLE.
Topics: Animals; Cathepsins; Cattle; Chloromercuribenzoates; Chromatography; Cysteine; Edetic Acid; Electrophoresis; Ethylmaleimide; Fishes; Hemoglobins; Hydrogen-Ion Concentration; Iodoacetates; Iron; Isoflurophate; Kinetics; Muramidase; Muscles; Peptide Hydrolases; Research; Serum Albumin; Serum Albumin, Bovine; Spectrophotometry | 1964 |
MACROMOLECULAR REPAIR AND FREE RADICAL SCAVENGING IN THE PROTECTION OF BACTERIA AGAINST X-RAYS.
Topics: Carbon Dioxide; Chemical Phenomena; Chemistry; Cysteine; Escherichia coli; Ethylmaleimide; Hydroxides; Macromolecular Substances; Nitric Oxide; Nitrogen; Oxygen; Pyrroles; Radiation Effects; Radiation-Protective Agents; Research; Sulfhydryl Compounds; X-Rays | 1965 |
STUDIES ON THE ACTIVE SITE OF THE A PROTEIN SUBUNIT OF THE ESCHERICHIA COLI TRYPTOPHAN SYNTHETASE.
Topics: Bacterial Proteins; Biochemical Phenomena; Biochemistry; Carbon Isotopes; Catalysis; Catalytic Domain; Cysteine; Enzyme Inhibitors; Escherichia coli; Ethylmaleimide; Histidine; Hydro-Lyases; Iodoacetates; Kinetics; Light; Methionine; Molecular Biology; Protein Subunits; Pyrroles; Research; Tryptophan Synthase; Urea | 1965 |
THE INHIBITION OF THE URIDINE DIPHOSPHATE-TRANSGLUCURONYLASE ACTIVITY OF MOUSE-LIVER HOMOGENATES BY THIOL REAGENTS.
Topics: Amides; Aminophenols; Arsenicals; Benzoates; Chloromercuribenzoates; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Glucosyltransferases; Glucuronates; Glutathione; Humans; Liver; Metabolism; Mice; Nitrophenols; Pharmacology; Phenols; Pyrroles; Research; Sulfhydryl Reagents; Uridine; Uridine Diphosphate | 1965 |
Identification of the dimer interface of the lactose transport protein from Streptococcus thermophilus.
Topics: Amino Acid Sequence; Binding Sites; Biological Transport; Cross-Linking Reagents; Cysteine; Cytoplasm; Detergents; Dimerization; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Immunoblotting; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutation; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; Protons; Streptococcus; Symporters; Time Factors | 2003 |
Covalent modification of cysteine 193 impairs ATPase function of nucleotide-binding domain of a Candida drug efflux pump.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Candida; Catalysis; Cations; Cysteine; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethylmaleimide; Hydrolysis; Iodoacetic Acid; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Structure, Tertiary; Sequence Homology, Amino Acid | 2003 |
Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolytic activity of CvaB, an ATP-binding cassette transporter.
Topics: Amino Acid Sequence; Animals; ATP-Binding Cassette Transporters; Blotting, Western; Calcium; Calpain; Cell Division; Colicins; Culture Media; Cysteine; Cysteine Endopeptidases; Dose-Response Relationship, Drug; Edetic Acid; Egtazic Acid; Escherichia coli Proteins; Ethylmaleimide; Histidine; Humans; Hydrogen-Ion Concentration; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Peptides; Plasmids; Protease Inhibitors; Protein Binding; Protein Structure, Tertiary; Rats; Sequence Homology, Amino Acid; Time Factors | 2004 |
Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport.
Topics: Amino Acid Sequence; Blotting, Western; Carrier Proteins; Cross-Linking Reagents; Cysteine; Dimerization; Drug Resistance, Microbial; Drug Resistance, Multiple; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Proteins; Models, Chemical; Models, Molecular; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Mutation; Plasmids; Protein Binding; Protein Conformation; Pyridinium Compounds; Sequence Homology, Amino Acid; Time Factors | 2004 |
Sensitivity of lysosomal enzymes to the plant alkaloid sanguinarine: comparison with other SH-specific agents.
Topics: Acetylglucosaminidase; Acid Phosphatase; Alkaloids; Animals; Arylsulfatases; Benzophenanthridines; beta-Galactosidase; Cell Line; Cysteine; Dose-Response Relationship, Drug; Enzyme Inhibitors; Ethylmaleimide; Hydrolases; Isoquinolines; Lipase; Lysosomes; Mice; Oxidation-Reduction; p-Chloromercuribenzoic Acid; Sulfhydryl Compounds; Sulfhydryl Reagents | 2003 |
Aqueous access pathways in subunit a of rotary ATP synthase extend to both sides of the membrane.
Topics: Adenosine Triphosphate; Aspartic Acid; Biological Transport; Cell Membrane; Cysteine; Cystine; Cytoplasm; Escherichia coli; Ethylmaleimide; Models, Molecular; Mutation; Protein Structure, Tertiary; Proton-Translocating ATPases; Protons; Silver; Temperature; Time Factors | 2003 |
Cysteine-scanning mutagenesis of transmembrane segment 1 of glucose transporter GLUT1: extracellular accessibility of helix positions.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Animals; Cell Membrane; Cell Membrane Permeability; Cysteine; Ethylmaleimide; Extracellular Fluid; Glucose; Glucose Transporter Type 1; Humans; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Oocytes; Peptide Fragments; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Transport; Sulfhydryl Reagents; Xenopus | 2004 |
Ratiometric pulsed alkylation mass spectrometry as a probe of thiolate reactivity in different metalloderivatives of Staphylococcus aureus pI258 CadC.
Topics: Alkylation; Apoproteins; Bacterial Proteins; Bismuth; Cadmium; Chymotrypsin; Cobalt; Cysteine; Ethylmaleimide; Hydrolysis; Metalloproteins; Peptide Fragments; Plasmids; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staphylococcus aureus; Sulfhydryl Compounds; Trypsin; Zinc | 2004 |
Spectroscopic characterization of heat-induced nonnative beta-lactoglobulin monomers.
Topics: Animals; Cattle; Cysteine; Ethylmaleimide; Hot Temperature; Lactoglobulins; Peptide Mapping; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrum Analysis | 2004 |
Oxidative stress inhibits MEKK1 by site-specific glutathionylation in the ATP-binding domain.
Topics: Adenosine Triphosphate; Alkylation; Amino Acid Sequence; Amino Acid Substitution; Binding Sites; Catalytic Domain; Cell Line, Tumor; Cysteine; Dithiothreitol; Enzyme Inhibitors; Ethylmaleimide; Glutathione; Humans; Lymph Nodes; Male; MAP Kinase Kinase Kinase 1; Molecular Sequence Data; Mutation; Oxidants; Oxidation-Reduction; Oxidative Stress; Peptides; Prostatic Neoplasms; Protein Structure, Tertiary; Valine | 2004 |
Two conserved cysteine residues are critical for the enzymic function of the human platelet-derived growth factor receptor-beta: evidence for different roles of Cys-822 and Cys-940 in the kinase activity.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Carcinoma, Hepatocellular; Cell Line; Cell Line, Tumor; Cysteine; Cytoplasm; Disulfides; DNA, Neoplasm; Ethylmaleimide; Humans; Insecta; Kidney; Liver Neoplasms; Mass Spectrometry; Molecular Sequence Data; Mutation; Peptides; Phosphotransferases; Protein Conformation; Protein Structure, Tertiary; Protein-Tyrosine Kinases; Receptor, Platelet-Derived Growth Factor beta | 2004 |
Single-channel analysis of KCNQ K+ channels reveals the mechanism of augmentation by a cysteine-modifying reagent.
Topics: Animals; Biotinylation; Cells, Cultured; CHO Cells; Cricetinae; Cysteine; Dose-Response Relationship, Drug; Ethylmaleimide; Green Fluorescent Proteins; Humans; KCNQ Potassium Channels; KCNQ2 Potassium Channel; KCNQ3 Potassium Channel; Luminescent Proteins; Membrane Potentials; Microscopy, Fluorescence; Mutagenesis, Site-Directed; Patch-Clamp Techniques; Potassium Channel Blockers; Potassium Channels; Potassium Channels, Voltage-Gated; Rats; Recombinant Fusion Proteins; Sulfhydryl Reagents; Transfection | 2004 |
Role of cysteine residues of p65/NF-kappaB on the inhibition by the sesquiterpene lactone parthenolide and N-ethyl maleimide, and on its transactivating potential.
Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Calcium-Binding Proteins; Cysteine; DNA-Binding Proteins; Ethylmaleimide; HeLa Cells; Humans; I-kappa B Kinase; Jurkat Cells; Macrophages; Membrane Glycoproteins; Mice; Mutation; Nerve Tissue Proteins; NF-kappa B; Protein Serine-Threonine Kinases; Sesquiterpenes; Synaptotagmin I; Synaptotagmins; Transcription Factor RelA; Transcriptional Activation; Transfection | 2004 |
Thermal stability of beta-lactoglobulins A and B: effect of SDS, urea, cysteine and N-ethylmaleimide.
Topics: Calorimetry, Differential Scanning; Cysteine; Drug Stability; Ethylmaleimide; Hot Temperature; Hydrogen-Ion Concentration; Lactoglobulins; Protein Denaturation; Protein Structure, Secondary; Sodium Dodecyl Sulfate; Spectroscopy, Fourier Transform Infrared; Urea | 2004 |
The fourth transmembrane domain of the Helicobacter pylori Na+/H+ antiporter NhaA faces a water-filled channel required for ion transport.
Topics: Alanine; Amino Acid Sequence; Antiporters; Aspartic Acid; Binding Sites; Cell Membrane; Cysteine; Cytoplasm; DNA; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Helicobacter pylori; Ion Transport; Ions; Kinetics; Leucine; Lithium; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Phenylalanine; Plasmids; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Protons; Serine; Sodium-Hydrogen Exchangers; Threonine; Valine; Water | 2004 |
Mapping of factor XIII solvent accessibility as a function of activation state using chemical modification methods.
Topics: Acetic Anhydrides; Acetylation; Alkylation; Amino Acid Sequence; Cysteine; Ethylmaleimide; Factor XIII; Factor XIIIa; Humans; Hydrolysis; Intercellular Signaling Peptides and Proteins; Iodoacetamide; Lysine; Molecular Sequence Data; Peptide Mapping; Peptides; Solutions; Solvents; Trypsin | 2004 |
Targeted mutagenesis of the Mycobacterium smegmatis mca gene, encoding a mycothiol-dependent detoxification protein.
Topics: Amidohydrolases; Anti-Bacterial Agents; Bridged Bicyclo Compounds; Cysteine; Dinitrochlorobenzene; Disaccharides; Enzyme Inhibitors; Ethylmaleimide; Gene Deletion; Genes, Bacterial; Genetic Complementation Test; Glycopeptides; Inositol; Iodoacetamide; Mutagenesis, Insertional; Mycobacterium smegmatis; Mycobacterium tuberculosis; Naphthoquinones; Oxidants; Pyrazoles; Rifamycins; Streptomycin; Sulfhydryl Compounds; Vitamin K 3 | 2004 |
Induction of mitochondrial fusion by cysteine-alkylators ethacrynic acid and N-ethylmaleimide.
Topics: Animals; Antioxidants; Ascorbic Acid; Cell Line; Cell Survival; Cricetinae; Cysteine; Ethacrynic Acid; Ethylmaleimide; Glutathione; Membrane Fusion; Mitochondria; Molecular Structure; Sulfhydryl Reagents; Vitamin E | 2005 |
Differential protein mobility of the gamma-aminobutyric acid, type A, receptor alpha and beta subunit channel-lining segments.
Topics: Animals; Binding Sites; Cysteine; Diazepam; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Electric Conductivity; Ethylmaleimide; gamma-Aminobutyric Acid; Inhibitory Concentration 50; Models, Molecular; Movement; Oocytes; Oxidation-Reduction; Protein Conformation; Protein Subunits; Rats; Receptors, GABA-A; RNA, Messenger; Zinc | 2005 |
Enhanced dephosphorylation of cAMP-dependent protein kinase by oxidation and thiol modification.
Topics: Animals; Binding Sites; Blotting, Western; Cyclic AMP-Dependent Protein Kinases; Cysteine; Disulfides; Ethylmaleimide; Glutathione; Mice; Models, Molecular; Mutation; Oxidative Stress; Oxygen; PC12 Cells; Phosphorylation; Protein Conformation; Protein Kinase C; Protein Kinase C-alpha; Protein Structure, Tertiary; Rats; Sulfhydryl Compounds; Threonine; Time Factors | 2005 |
Molecular characterization of the human Calpha-formylglycine-generating enzyme.
Topics: Amino Acid Sequence; Binding Sites; Blotting, Western; Catalytic Domain; Cell Line, Tumor; Cross-Linking Reagents; Cysteine; Dimerization; Disulfides; DNA, Complementary; Endoplasmic Reticulum; Ethylmaleimide; Fibroblasts; Fluorescent Antibody Technique, Indirect; Glycoside Hydrolases; Glycosylation; Humans; Microscopy, Immunoelectron; Molecular Sequence Data; Oxidation-Reduction; Oxidoreductases Acting on Sulfur Group Donors; Peptides; Plasmids; Polysaccharides; Proline; Protein Binding; Protein Processing, Post-Translational; Protein Structure, Tertiary; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfatases; Thermolysin; Time Factors; Trypsin; Two-Hybrid System Techniques | 2005 |
Accessibility of cysteines in the native bovine rod cGMP-gated channel.
Topics: Amino Acid Sequence; Animals; Cattle; Conserved Sequence; Cyclic GMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Ethylmaleimide; Eye Proteins; Ion Channels; Maleimides; Molecular Sequence Data; Polyethylene Glycols; Protein Subunits; Rod Cell Outer Segment; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2005 |
The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.
Topics: Amino Acid Sequence; Amino Acid Substitution; Cell Membrane; Cysteine; Cytoplasm; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphorylation; Protein Structure, Tertiary; Recombinant Proteins; Sulfhydryl Reagents; Trypsin | 2005 |
Differential effects of cysteine and methionine residues in the antioxidant activity of human serum albumin.
Topics: Antioxidants; Chloramines; Copper; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Ethylmaleimide; Free Radical Scavengers; Free Radicals; Hemolysis; Humans; Lipoproteins, LDL; Methionine; Oxidation-Reduction; Oxidative Stress; Oxygen; Phenanthrolines; Protein Binding; Serum Albumin; Time Factors; Tosyl Compounds | 2005 |
S-nitrosylation of N-ethylmaleimide sensitive factor mediates surface expression of AMPA receptors.
Topics: Adenylyl Imidodiphosphate; Aldehydes; Animals; Blotting, Western; Cells, Cultured; Cerebellum; Cysteine; Diagnostic Imaging; Dizocilpine Maleate; Drug Interactions; Embryo, Mammalian; Enzyme Activation; Enzyme Inhibitors; Epoxy Compounds; Ethylmaleimide; Excitatory Amino Acid Antagonists; Gene Expression Regulation; Hippocampus; Immunoprecipitation; Mice; Mice, Knockout; Mutagenesis; N-Ethylmaleimide-Sensitive Proteins; Nerve Tissue Proteins; Neurons; NG-Nitroarginine Methyl Ester; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase; Nitric Oxide Synthase Type I; Penicillamine; Protein Binding; Rats; Receptors, AMPA; Recombinant Fusion Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Reagents; Time Factors; Transfection; Vesicular Transport Proteins | 2005 |
Structural organization and interactions of transmembrane domains in tetraspanin proteins.
Topics: Algorithms; Amino Acid Motifs; Amino Acid Sequence; Antigens, CD; Blotting, Western; Cloning, Molecular; Conserved Sequence; Cross-Linking Reagents; Cysteine; Dimerization; Disulfides; DNA Mutational Analysis; Ethylmaleimide; Glycine; Humans; Leucine; Membrane Glycoproteins; Models, Molecular; Molecular Conformation; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Phenylalanine; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tetraspanin 29 | 2005 |
Formation of an antiparallel, intermolecular coiled coil is associated with in vivo dimerization of osmosensor and osmoprotectant transporter ProP in Escherichia coli.
Topics: Amino Acid Sequence; Amino Acid Substitution; Arginine; Cross-Linking Reagents; Cysteine; Dimerization; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Glutamic Acid; Isoleucine; Molecular Sequence Data; Mutagenesis, Site-Directed; Osmolar Concentration; Osmotic Pressure; Oxidation-Reduction; Peptide Fragments; Protein Structure, Secondary; Symporters | 2005 |
Cysteine modification by lipid peroxidation products inhibits protein disulfide isomerase.
Topics: Acrolein; Aldehydes; Amino Acid Sequence; Animals; Blotting, Western; Central Nervous System Depressants; Cysteine; Diet; Dietary Fats; Electrophoresis, Gel, Two-Dimensional; Ethanol; Ethylmaleimide; Hydrolysis; Insulin; Lipid Peroxidation; Male; Mass Spectrometry; Molecular Sequence Data; Oxidation-Reduction; Protein Disulfide-Isomerases; Rats; Rats, Sprague-Dawley; Sulfhydryl Reagents; Trypsin | 2005 |
Metallothionein disulfides are present in metallothionein-overexpressing transgenic mouse heart and increase under conditions of oxidative stress.
Topics: Animals; Antineoplastic Agents; Blotting, Western; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Doxorubicin; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Iodoacetamide; Mass Spectrometry; Metallothionein; Mice; Mice, Transgenic; Myocardium; Oxidative Stress; Peptides; Protein Structure, Tertiary; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors; Trypsin; Zinc | 2006 |
GEC1 interacts with the kappa opioid receptor and enhances expression of the receptor.
Topics: 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Analgesics, Non-Narcotic; Animals; Blotting, Western; Brain; Cell Membrane; CHO Cells; Cricetinae; Cysteine; DNA, Complementary; Down-Regulation; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Estrogens; Ethylmaleimide; Flow Cytometry; Gene Expression Regulation; Glutathione Transferase; Glycoside Hydrolases; Glycosylation; Golgi Apparatus; Histidine; Humans; Immunoblotting; Immunohistochemistry; Immunoprecipitation; Kinetics; Methionine; Microscopy, Fluorescence; Microtubule-Associated Proteins; Molecular Sequence Data; Protein Binding; Protein Structure, Tertiary; Rats; Receptors, Opioid, kappa; Recombinant Fusion Proteins; RNA, Messenger; Sequence Homology, Amino Acid; Signal Transduction; Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; Time Factors; Transfection; Tubulin; Two-Hybrid System Techniques | 2006 |
Site-directed alkylation of cysteine replacements in the lactose permease of Escherichia coli: helices I, III, VI, and XI.
Topics: Alkylation; Amino Acid Sequence; Cysteine; Escherichia coli Proteins; Ethylmaleimide; Liposomes; Mesylates; Monosaccharide Transport Proteins; Protein Structure, Secondary; Symporters | 2006 |
Proteomics approaches to study the redox state of cysteine-containing proteins.
Topics: Animals; Biotinylation; Cattle; Cysteine; Ethylmaleimide; HeLa Cells; Humans; Hydrogen-Ion Concentration; Nitroso Compounds; Ovalbumin; Oxidation-Reduction; Proteins; Proteomics; Ribonucleases; Serum Albumin, Bovine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Reagents | 2005 |
Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme.
Topics: Amphibian Proteins; Animals; Catalytic Domain; Chlorocebus aethiops; Cholinesterase Inhibitors; Cholinesterases; COS Cells; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Inhibitory Concentration 50; Models, Molecular; Mutagenesis, Site-Directed; Sequence Alignment; Sulfhydryl Reagents; Time Factors; Transfection | 2006 |
The paired domain of Pax3 contains a putative homeodomain interaction pocket defined by cysteine scanning mutagenesis.
Topics: Amino Acid Sequence; Animals; Base Sequence; Chlorocebus aethiops; COS Cells; Cysteine; DNA Primers; Electrophoretic Mobility Shift Assay; Ethylmaleimide; Molecular Sequence Data; Mutagenesis, Site-Directed; Paired Box Transcription Factors; PAX3 Transcription Factor | 2006 |
Human eosinophil major basic protein 2: location of disulfide bonds and free sulfhydryl groups.
Topics: Amino Acid Sequence; Blood Proteins; Cysteine; Cystine; Eosinophil Major Basic Protein; Ethylmaleimide; Humans; Peptide Mapping; Proteoglycans; Sequence Alignment; Spectrometry, Mass, Electrospray Ionization; Trypsin | 2007 |
Site-directed alkylation and the alternating access model for LacY.
Topics: Alkylating Agents; Alkylation; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Ligands; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Symporters | 2007 |
Aqueous access pathways in ATP synthase subunit a. Reactivity of cysteine substituted into transmembrane helices 1, 3, and 5.
Topics: Aspartic Acid; Cell Membrane; Cysteine; Cytoplasm; Escherichia coli; Ethylmaleimide; Mutation; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Proton-Translocating ATPases; Protons; Silver; Water | 2007 |
Conformationally sensitive reactivity to permeant sulfhydryl reagents of cysteine residues engineered into helical hairpin 1 of the glutamate transporter GLT-1.
Topics: Amino Acid Sequence; Biological Transport; Cell Membrane Permeability; Cross-Linking Reagents; Cysteine; Cytoplasm; Ethylmaleimide; Excitatory Amino Acid Transporter 2; HeLa Cells; Humans; Kainic Acid; Mesylates; Molecular Sequence Data; Mutant Proteins; Oxidation-Reduction; Phenanthrolines; Protein Engineering; Protein Structure, Secondary; Protein Structure, Tertiary; Sulfhydryl Reagents | 2007 |
The role of beta93 Cys in the inhibition of Hb S fiber formation.
Topics: Anemia, Sickle Cell; Cysteine; Ethylmaleimide; Hemoglobin, Sickle; Humans; Methemoglobin; Nitric Oxide; S-Nitrosothiols; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman | 2007 |
Inhibition of topoisomerase I cleavage activity by thiol-reactive compounds: importance of vicinal cysteines 504 and 505.
Topics: Arsenicals; Camptothecin; Cysteine; DNA Cleavage; DNA Topoisomerases, Type I; Enzyme Inhibitors; Ethylmaleimide; Humans; Mutagenesis, Site-Directed; Protein Conformation; Sulfhydryl Compounds; Topoisomerase I Inhibitors | 2007 |
Structure and function of transmembrane segment XII in osmosensor and osmoprotectant transporter ProP of Escherichia coli.
Topics: Amino Acid Sequence; Cross-Linking Reagents; Cysteine; Dimerization; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Models, Molecular; Molecular Sequence Data; Osmolar Concentration; Symporters | 2007 |
Neurotoxic mechanisms of electrophilic type-2 alkenes: soft soft interactions described by quantum mechanical parameters.
Topics: 1-Propanol; Acrolein; Acrylamide; Acrylates; Alkenes; Animals; Butanones; Corpus Striatum; Cysteine; Dopamine; Ethylmaleimide; Male; Neurotoxicity Syndromes; Propanols; Quantum Theory; Rats; Rats, Sprague-Dawley; Synaptosomes | 2007 |
Tissue factor coagulant function is enhanced by protein-disulfide isomerase independent of oxidoreductase activity.
Topics: Animals; Bacitracin; Binding Sites; Cattle; Cells, Cultured; Cysteine; Enzyme Activation; Ethylmaleimide; Factor VIIa; Factor X; Humans; Methyl Methanesulfonate; Molecular Chaperones; Multienzyme Complexes; Oxidation-Reduction; Phospholipids; Protein Disulfide-Isomerases; Protein Structure, Quaternary; Signal Transduction; Thromboplastin | 2007 |
Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins.
Topics: Affinity Labels; Alkylation; Biochemistry; Carbon Isotopes; Chromatography, Affinity; Cysteine; Electrophoresis; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Proteins; Monosaccharide Transport Proteins; Mutation; Solvents; Sulfhydryl Compounds; Symporters | 2007 |
Site-directed alkylation of LacY: effect of the proton electrochemical gradient.
Topics: Alkylating Agents; Alkylation; Cysteine; Electrochemistry; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Ligands; Membrane Potentials; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protons; Recombinant Proteins; Symporters | 2007 |
Subunit a facilitates aqueous access to a membrane-embedded region of subunit c in Escherichia coli F1F0 ATP synthase.
Topics: Adenosine Triphosphate; Amino Acid Substitution; Binding Sites; Carbon Radioisotopes; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Mesylates; Mitochondrial Proton-Translocating ATPases; Models, Molecular; Mutant Proteins; Protein Subunits; Proton Pumps; Silver; Staining and Labeling; Sulfhydryl Reagents | 2008 |
Modification of cysteine.
Topics: Acetamides; Acrylamide; Alkylation; Biochemistry; Colorimetry; Crystallization; Cysteine; Disulfides; Ethylmaleimide; Fluoroacetates; Formates; Molecular Weight; Oxidation-Reduction; Propylamines; Proteins; Pyridines; Trifluoroacetic Acid | 2001 |
Analysis of the membrane topology of transmembrane segments in the C-terminal hydrophobic domain of the yeast vacuolar ATPase subunit a (Vph1p) by chemical modification.
Topics: Cell Membrane; Cysteine; Cytoplasm; Detergents; Ethylmaleimide; Gene Expression Regulation, Fungal; Hydrophobic and Hydrophilic Interactions; Models, Biological; Mutagenesis, Site-Directed; Mutation; Phenotype; Protein Structure, Tertiary; Saccharomyces cerevisiae; Vacuolar Proton-Translocating ATPases | 2008 |
Analysis of the interaction between the UL11 and UL16 tegument proteins of herpes simplex virus.
Topics: Amino Acid Motifs; Amino Acid Substitution; Animals; Binding Sites; Cell Line; Chlorocebus aethiops; Cloning, Molecular; Conserved Sequence; Cysteine; Ethylmaleimide; Gene Expression; Herpesvirus 1, Human; Humans; Mutagenesis, Site-Directed; Protein Binding; Protein Interaction Domains and Motifs; Protein Interaction Mapping; Recombinant Proteins; Sequence Deletion; Serine; Viral Structural Proteins | 2008 |
Biosynthesis of phosphatidylcholine by human lysophosphatidylcholine acyltransferase 1.
Topics: 1-Acylglycerophosphocholine O-Acyltransferase; Amino Acid Sequence; Animals; Cell Line; Cloning, Molecular; Conserved Sequence; Cysteine; DNA, Complementary; Ethylmaleimide; Evolution, Molecular; Gene Expression Regulation, Enzymologic; Glycosylation; Humans; Immune Sera; Lung; Mice; Molecular Sequence Data; Mutation; Oxidation-Reduction; Phosphatidylcholines; Phylogeny; Pulmonary Surfactants; Respiration; RNA, Messenger; Sequence Homology, Amino Acid; Sulfhydryl Compounds | 2009 |
Use of mass spectrometry to probe the nucleophilicity of cysteinyl residues of prolyl hydroxylase domain 2.
Topics: Alkylating Agents; Amino Acid Substitution; Catalytic Domain; Cysteine; Escherichia coli; Ethylmaleimide; Gene Expression; Humans; Hypoxia-Inducible Factor-Proline Dioxygenases; Mesylates; Procollagen-Proline Dioxygenase; Recombinant Proteins; Spectrometry, Mass, Electrospray Ionization; Spin Labels; Sulfhydryl Compounds; Sulfhydryl Reagents | 2009 |
Thiol-dependent membrane transport of selenium through an integral protein of the red blood cell membrane.
Topics: Anion Exchange Protein 1, Erythrocyte; Biological Transport; Cell Membrane; Cysteine; Electrophoresis; Erythrocytes; Ethylmaleimide; Humans; Selenium; Sulfhydryl Compounds; Tetrathionic Acid | 2009 |
Molecular mechanism for H(2)S-induced activation of K(ATP) channels.
Topics: Air Pollutants; Cell Line; Chloramines; Cysteine; Ethylmaleimide; Humans; Hydrogen Sulfide; Ion Channel Gating; KATP Channels; Mutagenesis, Site-Directed; Oxidants; Patch-Clamp Techniques; Point Mutation; Protein Conformation; Protein Disulfide-Isomerases; Protein Subunits; Sulfhydryl Reagents; Tosyl Compounds | 2010 |
Identification of specific transmembrane residues and ligand-induced interface changes involved in homo-dimer formation of a yeast G protein-coupled receptor.
Topics: Amino Acid Substitution; Amino Acids; Binding Sites; Cell Membrane; Cell Proliferation; Cysteine; Cystine; Ethylmaleimide; Factor Xa; Genes, Reporter; Kinetics; Ligands; Mating Factor; Models, Molecular; Organometallic Compounds; Oxidants; Peptides; Phenanthrolines; Protein Multimerization; Receptors, Mating Factor; Recombinant Proteins; Saccharomyces cerevisiae Proteins | 2009 |
Homodimeric intrinsic membrane proteins. Identification and modulation of interactions between mitochondrial transporter (carrier) subunits.
Topics: Cysteine; DNA Mutational Analysis; Enzyme Inhibitors; Ethylmaleimide; Humans; Mitochondrial Membranes; Mutation; Phosphate Transport Proteins; Protein Multimerization; Protein Subunits | 2010 |
VDAC1 cysteine residues: topology and function in channel activity and apoptosis.
Topics: Animals; Apoptosis; Arsenic Trioxide; Arsenicals; Cattle; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Fluoresceins; Humans; Hydrogen Peroxide; Immunoblotting; Lipid Bilayers; Mice; Mitochondrial Membranes; Oxides; Rabbits; Rats; RNA, Small Interfering; Sodium Selenite; Structure-Activity Relationship; Voltage-Dependent Anion Channel 1 | 2010 |
Soybean (Glycine max) urease: significance of sulfhydryl groups in urea catalysis.
Topics: Biocatalysis; Cysteine; Dithionitrobenzoic Acid; Enzyme Assays; Ethylmaleimide; Glycine max; Iodoacetamide; Kinetics; p-Chloromercuribenzoic Acid; Plant Proteins; Sulfhydryl Compounds; Sulfhydryl Reagents; Time Factors; Urea; Urease | 2010 |
Collagenolytic activity in sonic extracts of Tannerella forsythia.
Topics: Bacteroides; Calcium Chloride; Cathepsins; Cell Line, Tumor; Chelating Agents; Collagen Type I; Cysteine; Cysteine Proteinase Inhibitors; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Inhibitors; Enzyme Precursors; Ethylmaleimide; Gelatinases; Humans; Iodoacetamide; Iodoacetic Acid; Leucine; Leupeptins; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Phenylmethylsulfonyl Fluoride; Protease Inhibitors; Serine Proteinase Inhibitors; Tosyllysine Chloromethyl Ketone; Tosylphenylalanyl Chloromethyl Ketone | 2010 |
Interactions of apurinic/apyrimidinic endonuclease with a redox inhibitor: evidence for an alternate conformation of the enzyme.
Topics: Animals; Benzoquinones; Cysteine; DNA-(Apurinic or Apyrimidinic Site) Lyase; Ethylmaleimide; Humans; Oxidation-Reduction; Propionates; Protein Conformation; Spectrometry, Mass, Electrospray Ionization; Sulfhydryl Reagents; Temperature | 2011 |
Identification of two reactive cysteine residues in the tumor suppressor protein p53 using top-down FTICR mass spectrometry.
Topics: Alkylating Agents; Amino Acid Sequence; Crystallography, X-Ray; Cysteine; Ethylmaleimide; Fourier Analysis; Humans; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Protein Unfolding; Temperature; Tumor Suppressor Protein p53 | 2011 |
Topology and accessibility of the transmembrane helices and the sensory site in the bifunctional transporter DcuB of Escherichia coli.
Topics: Alkaline Phosphatase; Amino Acid Sequence; Bacterial Proteins; Catalytic Domain; Cell Membrane; Cysteine; Dicarboxylic Acid Transporters; Escherichia coli K12; Escherichia coli Proteins; Ethylmaleimide; Hydrophobic and Hydrophilic Interactions; Lac Operon; Models, Molecular; Molecular Sequence Data; Polyethylene Glycols; Protein Structure, Secondary; Recombinant Fusion Proteins; Stilbenes; Sulfonic Acids | 2011 |
An assay to quantitate reducible cysteines from nanograms of GST-fusion proteins.
Topics: Cysteine; Escherichia coli; Ethylmaleimide; Glutathione Transferase; Luminescent Measurements; Oxidation-Reduction; Protein Processing, Post-Translational; Proteins; Recombinant Fusion Proteins; SNARE Proteins; Streptavidin | 2011 |
Definition of membrane topology and identification of residues important for transport in subunit a of the vacuolar ATPase.
Topics: Binding Sites; Biological Transport; Cell Membrane; Cysteine; Cytoplasm; Ethylmaleimide; Fungal Proteins; Gene Expression Regulation, Fungal; Mutagenesis, Site-Directed; Mutation; Phenotype; Protein Structure, Tertiary; Vacuolar Proton-Translocating ATPases | 2011 |
Capturing of the free cysteine residue in the ligand-binding site by affinity labeling of the ORL1 nociceptin receptor.
Topics: Affinity Labels; Amino Acid Sequence; Animals; Binding Sites; Chlorocebus aethiops; COS Cells; Cysteine; Ethylmaleimide; Ligands; Narcotic Antagonists; Nociceptin Receptor; Peptides; Protein Binding; Receptors, Opioid | 2011 |
Conjugation of anticancer drugs through endogenous monoclonal antibody cysteine residues.
Topics: Animals; Antibodies, Monoclonal; Antigens; Antineoplastic Agents; Cell Line, Tumor; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Cysteine; Disulfides; Drug Carriers; Ethylmaleimide; Humans; Hybridomas; Immobilized Proteins; Immunoconjugates; Mice; Molecular Structure; Neoplasms; Oxidation-Reduction; Sulfhydryl Reagents | 2012 |
Nitric oxide, peroxynitrite, S-nitrosothiols and thiols are unlikely to exert their effects on recombinant cyclooxygenase-1 and cyclooxygenase-2 activity in vitro by modifying cysteine moieties.
Topics: Arachidonic Acid; Blood Platelets; Cyclooxygenase 1; Cyclooxygenase 2; Cysteine; Ethylmaleimide; Humans; Nitric Oxide; Peroxynitrous Acid; Recombinant Proteins; S-Nitrosothiols | 2012 |
Possible involvement of S-nitrosylation of brain cyclooxygenase-1 in bombesin-induced central activation of adrenomedullary outflow in rats.
Topics: 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; Adrenal Medulla; Animals; Benzoates; Bombesin; Brain; Catecholamines; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Ethylmaleimide; Imidazoles; Injections, Intraventricular; Male; Molsidomine; Nitric Oxide Donors; Oxadiazoles; Paraventricular Hypothalamic Nucleus; Quinoxalines; Rats; Rats, Wistar; S-Nitrosothiols; Sulfhydryl Reagents | 2012 |
Disulfide linkages mediating nucleocapsid protein dimerization are not required for porcine arterivirus infectivity.
Topics: Amino Acid Sequence; Animals; Cell Line; Cysteine; Ethylmaleimide; Gene Order; Molecular Sequence Data; Mutation; Nucleocapsid Proteins; Porcine respiratory and reproductive syndrome virus; Protein Binding; Protein Multimerization; Sequence Alignment; Virion | 2012 |
Evidence for the importance of OxPAPC interaction with cysteines in regulating endothelial cell function.
Topics: Binding Sites; Cells, Cultured; Cysteine; Endothelial Cells; Ethylmaleimide; Humans; Isoprostanes; Phosphatidylcholines; Prostaglandin D2 | 2012 |
Functional consequences of sulfhydryl modification of the γ-aminobutyric acid transporter 1 at a single solvent-exposed cysteine residue.
Topics: Animals; Cysteine; Dithiothreitol; Ethylmaleimide; Female; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Gene Expression; Humans; Kinetics; Mesylates; Mutation; Oocytes; Patch-Clamp Techniques; Rhodamines; Sodium Chloride; Sulfhydryl Reagents; Transfection; Valproic Acid; Xenopus laevis | 2012 |
Reversible inactivation of dihydrolipoamide dehydrogenase by mitochondrial hydrogen peroxide.
Topics: Animals; Antimycin A; Brain; Catalase; Cysteine; Dihydrolipoamide Dehydrogenase; Dithiothreitol; Electron Transport Complex I; Electron Transport Complex III; Ethylmaleimide; Glutathione; Hydrogen Peroxide; Malates; Male; Mitochondria; Oxidation-Reduction; Oxidative Stress; Pyruvic Acid; Rats; Rats, Sprague-Dawley; Rotenone; Succinic Acid; Superoxides | 2013 |
The role of cysteines and histidins of the norepinephrine transporter.
Topics: Cysteine; Ethylmaleimide; Fluoxetine; HEK293 Cells; Histidine; Humans; Mutagenesis, Site-Directed; Norepinephrine Plasma Membrane Transport Proteins; Radioligand Assay; Subcellular Fractions | 2013 |
A novel cell lysis approach reveals that caspase-2 rapidly translocates from the nucleus to the cytoplasm in response to apoptotic stimuli.
Topics: Apoptosis; Caspase 2; Cell Line; Cell Nucleus; Cysteine; Cytoplasm; Ethylmaleimide; Humans; Mutation; Oxidative Stress; Protein Transport | 2013 |
Identification of cysteine residues in human cationic amino acid transporter hCAT-2A that are targets for inhibition by N-ethylmaleimide.
Topics: Amino Acid Substitution; Amino Acid Transport Systems, Basic; Animals; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Humans; Mutation, Missense; Oocytes; Protein Structure, Tertiary; Xenopus laevis | 2013 |
Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids.
Topics: Ascorbic Acid; Cyclohexanones; Cysteine; Dithiothreitol; Ethylmaleimide; Iodoacetamide; Methyl Methanesulfonate; Oxidation-Reduction; Proteins; Reactive Oxygen Species; Spectrometry, Mass, Electrospray Ionization; Sulfenic Acids; Sulfhydryl Compounds | 2013 |
Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications.
Topics: Animals; Cysteine; Ethylmaleimide; Mice; Oxidation-Reduction; Proteomics; Sepharose; Signal Transduction; Sulfhydryl Compounds | 2014 |
Hydrogen sulfide is essential for Schwann cell responses to peripheral nerve injury.
Topics: Animals; Axotomy; Cystathionine beta-Synthase; Cystathionine gamma-Lyase; Cysteine; Ethylmaleimide; Hydrogen Sulfide; Male; Mice; Mice, Inbred C57BL; Organ Culture Techniques; Peripheral Nerve Injuries; Reverse Transcriptase Polymerase Chain Reaction; Schwann Cells; Sciatic Nerve; Sulfurtransferases; Transaminases; Wallerian Degeneration | 2015 |
Role of Cys³⁶⁰² in the function and regulation of the cardiac ryanodine receptor.
Topics: Alkylation; Amino Acid Sequence; Amino Acid Substitution; Animals; Calcium Signaling; Calmodulin; Conserved Sequence; Cysteine; Disulfides; Ethylmaleimide; HEK293 Cells; Humans; Kinetics; Mice; Oxidation-Reduction; Peptide Fragments; Point Mutation; Protein Interaction Domains and Motifs; Pyridines; Recombinant Proteins; Ryanodine Receptor Calcium Release Channel; Sulfhydryl Reagents | 2015 |
Light-evoked S-nitrosylation in the retina.
Topics: Adaptation, Physiological; Animals; Cysteine; Enzyme Inhibitors; Ethylmaleimide; Female; Goldfish; Light; Male; Mice, Inbred C57BL; Mice, Transgenic; Nitric Oxide; Nitric Oxide Synthase Type I; Photic Stimulation; Retina; S-Nitrosothiols; Species Specificity; Tissue Culture Techniques | 2015 |
Neuronal acid-induced [Zn²⁺]i elevations calibrated using the low-affinity ratiometric probe FuraZin-1.
Topics: Acids; Adenosine Triphosphate; Animals; Calcium; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; Cells, Cultured; Cerebral Cortex; Chelating Agents; Cysteine; Cytosol; Dose-Response Relationship, Drug; Egtazic Acid; Embryo, Mammalian; Enzyme Inhibitors; Ethylenediamines; Ethylmaleimide; Magnesium; Mice; Mice, Inbred C57BL; Neurons; Polycyclic Compounds; Proton Ionophores; Zinc | 2015 |
Nanoscale UiO-MOF-based luminescent sensors for highly selective detection of cysteine and glutathione and their application in bioimaging.
Topics: Cysteine; Ethylmaleimide; Fluorescent Dyes; Glutathione; HeLa Cells; Humans; Maleimides; Models, Chemical; Organometallic Compounds; Porosity; Zirconium | 2015 |
Competitive kinetics as a tool to determine rate constants for reduction of ferrylmyoglobin by food components.
Topics: Catechin; Cysteine; Ethylmaleimide; Kinetics; Meat; Metmyoglobin; Oxidation-Reduction; Plant Extracts; Tea | 2016 |
Identification of a Disulfide Bridge in Sodium-Coupled Neutral Amino Acid Transporter 2(SNAT2) by Chemical Modification.
Topics: Alanine; Amino Acid Transport System A; Amino Acid Transport Systems; Animals; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; HEK293 Cells; Humans; Ion Transport; Mutagenesis; Mutation; Polyethylene Glycols; Rats; Sodium; Sulfhydryl Compounds | 2016 |
Modification of Cysteine.
Topics: Alkylation; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Formates; Indicators and Reagents; Iodoacetamide; Iodoacetic Acid; Oxidation-Reduction; Peptides; Proteins; Pyridines | 2017 |
Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.
Topics: Acylation; Alkenes; Arabidopsis; Butanes; Cysteine; Ethylmaleimide; Lipoylation; Maleimides; Protein Processing, Post-Translational; Proteins; Reproducibility of Results | 2017 |
Topological Dissection of the Membrane Transport Protein Mhp1 Derived from Cysteine Accessibility and Mass Spectrometry.
Topics: Bacterial Proteins; Binding Sites; Cysteine; Ethylmaleimide; Hydantoins; Kinetics; Mass Spectrometry; Membrane Transport Proteins; Micrococcaceae; Molecular Dynamics Simulation; Mutagenesis, Site-Directed; Protein Binding; Sodium; Substrate Specificity | 2017 |
Molecular mechanism and characterization of self-assembly of feather keratin gelation.
Topics: Animals; Chickens; Cysteine; Ethylmaleimide; Feathers; Hydrogel, Polyethylene Glycol Dimethacrylate; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Keratins; Oxidation-Reduction; Rheology; Sulfhydryl Compounds; Temperature; Viscoelastic Substances | 2018 |
Negative modulation of the GABA
Topics: Animals; Binding, Competitive; Chlorides; Cysteine; Cystine; Dose-Response Relationship, Drug; Ethylmaleimide; GABA-A Receptor Antagonists; gamma-Aminobutyric Acid; Homocysteine; Humans; Ion Transport; Oocytes; Patch-Clamp Techniques; Receptors, GABA-A; Recombinant Proteins; RNA, Complementary; Xenopus laevis | 2018 |
Analysis of Disulfide Bond Formation.
Topics: Animals; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Endosomes; Ethylmaleimide; HEK293 Cells; Humans; Immunoprecipitation; Methionine; Oxidation-Reduction; Protein Biosynthesis; Protein Folding; Staining and Labeling; Sulfur Radioisotopes | 2017 |
Proteomic profiling of oxidized cysteine and methionine residues by hydroxyl radicals in myosin of pork.
Topics: Animals; Chromatography, Liquid; Cysteine; Disulfides; Ethylmaleimide; Hydrogen Peroxide; Hydroxyl Radical; Methionine; Muscle, Skeletal; Myosins; Oxidation-Reduction; Proteomics; Red Meat; Sulfones; Swine | 2018 |
Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolysaccharide Biosynthesis.
Topics: Bacteria; Bacterial Proteins; Biosynthetic Pathways; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Genes, Bacterial; Lipopolysaccharides; Maleimides; Mutagenesis, Site-Directed; Polyethylene Glycols; Staining and Labeling; Sulfhydryl Compounds | 2019 |
Stability of nitroxide biradical TOTAPOL in biological samples.
Topics: Bacteria; Cyclic N-Oxides; Cysteine; Electron Spin Resonance Spectroscopy; Escherichia coli; Ethylmaleimide; Ferricyanides; Free Radicals; Magnetic Resonance Spectroscopy; Nitrogen Oxides; Oxidative Phosphorylation; Propanols; Temperature | 2019 |
Highly specific monitoring and imaging of endogenous and exogenous cysteine in living cells.
Topics: Coumarins; Cysteine; Ethylmaleimide; Fluorescent Dyes; HeLa Cells; Humans; Hydrogen Peroxide; Hydrogen-Ion Concentration; Limit of Detection; Microscopy, Confocal; Microscopy, Fluorescence | 2019 |
Enhancing the peroxidase-like activity of ficin by rational blocking thiol groups for colorimetric detection of biothiols.
Topics: Benzidines; Chromogenic Compounds; Colorimetry; Cysteine; Ethylmaleimide; Ficain; Glutathione; Homocysteine; Humans; Hydrogen Peroxide; Indicators and Reagents; Kinetics; Limit of Detection; Peroxidases; Phosphines | 2019 |
NEM assisted real-time fluorescence detection of Cys in cytoplasm and mice imaging by a Coumarin probe containing carboxyl group.
Topics: Animals; Cell Membrane Permeability; Computer Systems; Cysteine; Cytoplasm; Ethylmaleimide; Fluorescence; Fluorescent Dyes; Hep G2 Cells; Humans; Intravital Microscopy; Mice; Mice, Inbred BALB C; Molecular Structure; Optical Imaging; Sulfhydryl Reagents | 2020 |
Endogenous Cys-Assisted GSH@AgNCs-rGO Nanoprobe for Real-Time Monitoring of Dynamic Change in GSH Levels Regulated by Natural Drug.
Topics: Cysteine; Doxorubicin; Drugs, Chinese Herbal; Ethylmaleimide; Fluorescent Dyes; Glutathione; Graphite; Hep G2 Cells; Humans; Limit of Detection; Metal Nanoparticles; Reactive Oxygen Species; Silver; Spectrometry, Fluorescence | 2020 |
A near-infrared excitation/emission fluorescent probe for imaging of endogenous cysteine in living cells and zebrafish.
Topics: Animals; Cysteine; Ethylmaleimide; Fluorescent Dyes; Hep G2 Cells; Humans; Spectroscopy, Near-Infrared; Spectrum Analysis; Zebrafish | 2020 |
Tyrosine hydroxylase activity is regulated through the modification of the 176th cysteine residue.
Topics: Cysteine; Dopamine; Enzyme Activation; Ethylmaleimide; Humans; Mutation; Phosphorylation; Structure-Activity Relationship; Tyrosine 3-Monooxygenase | 2022 |
Characterization and structure of the human lysine-2-oxoglutarate reductase domain, a novel therapeutic target for treatment of glutaric aciduria type 1.
Topics: Amino Acid Metabolism, Inborn Errors; Animals; Brain Diseases, Metabolic; Cysteine; Ethylmaleimide; Glutaryl-CoA Dehydrogenase; Humans; Lysine; Mice; Saccharopine Dehydrogenases | 2022 |