esculetin and 4-7-10-13-eicosatetraynoic-acid

esculetin has been researched along with 4-7-10-13-eicosatetraynoic-acid* in 2 studies

Other Studies

2 other study(ies) available for esculetin and 4-7-10-13-eicosatetraynoic-acid

Article	Year
Biosynthesis of 12(S)-hydroxyeicosatetraenoic acid by bovine corneal epithelium. Acta physiologica Scandinavica, 1993, Volume: 147, Issue:1 Microsomes of bovine corneal epithelium metabolized [14C]arachidonic acid extensively to [14C]12-hydroxyeicosatetraenoic acid. The biosynthesis was not stimulated by exogenous NADPH and it could be inhibited by over 90% with two lipoxygenase inhibitors, esculetin and eicosatetraynoic acid. Chiral phase high performance liquid chromatography showed that 12-HETE consisted of the 12S enantiomer. The results indicate biosynthesis by 12S-lipoxygenase of bovine corneal epithelium rather than by cytochrome P450. Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 5,8,11,14-Eicosatetraynoic Acid; Animals; Cattle; Chromatography, High Pressure Liquid; Cytochrome P-450 Enzyme System; Endothelium, Corneal; Gas Chromatography-Mass Spectrometry; Hydroxyeicosatetraenoic Acids; In Vitro Techniques; Lipoxygenase; Lipoxygenase Inhibitors; Microsomes; Molecular Conformation; NADP; Umbelliferones	1993
Differential effects of putative inhibitors on cytosolic and membrane associated platelet lipoxygenase. Prostaglandins, leukotrienes, and medicine, 1987, Volume: 26, Issue:3 The effects of Indomethacin, Esculetin, ETYA (4, 7, 10, 13-eicosatetraynoic acid, U53119), 3-amino-1-trifluoromethyl-7-phenyl-pyrazoline (BW 755C), Quercetin, Phenidone, and Nordihydroguaretic acid (NDGA) on the synthesis of 12-L hydroxyeicosatetraenoic acid (12-HETE) by human platelet 12-lipoxygenases were investigated. Except Indomethacin and Esculetin, all other drugs demonstrated significant inhibitory effect on 12-lipoxygenase activity. The rank order of potency for the inhibition of lipoxygenase in intact human platelets was ETYA greater than Quercetin greater than NDGA greater than Esculetin greater than Indomethacin. BW755C and Indomethacin were effective against platelet cyclooxygenase also. ETYA (U53119) was the most potent and selective inhibitor of platelet lipoxygenases. Results of our studies suggest that known lipoxygenase inhibitors display differential effects on platelet cyclooxygenase as well as membrane and cytosol associated lipoxygenases. Topics: 4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; 5,8,11,14-Eicosatetraynoic Acid; Adult; Arachidonate Lipoxygenases; Blood Platelets; Cell Membrane; Cytosol; Humans; Indomethacin; Lipoxygenase Inhibitors; Masoprocol; Prostaglandin Antagonists; Pyrazoles; Quercetin; Substrate Specificity; Umbelliferones	1987

Article

Year

Biosynthesis of 12(S)-hydroxyeicosatetraenoic acid by bovine corneal epithelium.

Acta physiologica Scandinavica, 1993, Volume: 147, Issue:1

Microsomes of bovine corneal epithelium metabolized [14C]arachidonic acid extensively to [14C]12-hydroxyeicosatetraenoic acid. The biosynthesis was not stimulated by exogenous NADPH and it could be inhibited by over 90% with two lipoxygenase inhibitors, esculetin and eicosatetraynoic acid. Chiral phase high performance liquid chromatography showed that 12-HETE consisted of the 12S enantiomer. The results indicate biosynthesis by 12S-lipoxygenase of bovine corneal epithelium rather than by cytochrome P450.

Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 5,8,11,14-Eicosatetraynoic Acid; Animals; Cattle; Chromatography, High Pressure Liquid; Cytochrome P-450 Enzyme System; Endothelium, Corneal; Gas Chromatography-Mass Spectrometry; Hydroxyeicosatetraenoic Acids; In Vitro Techniques; Lipoxygenase; Lipoxygenase Inhibitors; Microsomes; Molecular Conformation; NADP; Umbelliferones

1993

Differential effects of putative inhibitors on cytosolic and membrane associated platelet lipoxygenase.

Prostaglandins, leukotrienes, and medicine, 1987, Volume: 26, Issue:3

The effects of Indomethacin, Esculetin, ETYA (4, 7, 10, 13-eicosatetraynoic acid, U53119), 3-amino-1-trifluoromethyl-7-phenyl-pyrazoline (BW 755C), Quercetin, Phenidone, and Nordihydroguaretic acid (NDGA) on the synthesis of 12-L hydroxyeicosatetraenoic acid (12-HETE) by human platelet 12-lipoxygenases were investigated. Except Indomethacin and Esculetin, all other drugs demonstrated significant inhibitory effect on 12-lipoxygenase activity. The rank order of potency for the inhibition of lipoxygenase in intact human platelets was ETYA greater than Quercetin greater than NDGA greater than Esculetin greater than Indomethacin. BW755C and Indomethacin were effective against platelet cyclooxygenase also. ETYA (U53119) was the most potent and selective inhibitor of platelet lipoxygenases. Results of our studies suggest that known lipoxygenase inhibitors display differential effects on platelet cyclooxygenase as well as membrane and cytosol associated lipoxygenases.

Topics: 4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; 5,8,11,14-Eicosatetraynoic Acid; Adult; Arachidonate Lipoxygenases; Blood Platelets; Cell Membrane; Cytosol; Humans; Indomethacin; Lipoxygenase Inhibitors; Masoprocol; Prostaglandin Antagonists; Pyrazoles; Quercetin; Substrate Specificity; Umbelliferones

1987