ergoline and ethyl-acetate

A method is described for the determination of roquefortine C in (blue) cheese. After liquid-liquid extraction with a mixture of hydrochloric acid and methanol, and filtration, an aliquot is analysed using column-switching reversed-phase liquid chromatography. The recovery of roquefortine C in Fetta cheese is about 85%, the calibration curve is linear from 10 to 2500 ng g(-1) (r2 = 0.998), and the detection limit is about 10 ng g(-1). In different batches of Danish Blue concentrations of 1000-2000 ng g(-1) of roquefortine C are found. As regards the stability of roquefortine C its half-life in diffuse daylight is ca. 50 min, while after irradiation with ultraviolet light, it is about 10 min.

Topics: Acetates; Buffers; Calibration; Cheese; Chromatography, Liquid; Drug Stability; Ergolines; Heterocyclic Compounds, 4 or More Rings; Hydrochloric Acid; Indoles; Methanol; Mycotoxins; Piperazines; Regression Analysis; Reproducibility of Results; Sensitivity and Specificity; Sunlight; Ultraviolet Rays

The influence of five doses of the D-2 receptor agonist quinpirole (0.025, 0.05, 0.075, 0.10, and 0.20 mg/kg IP) on the odor detection performance of 21 adult male Long Evans rats was assessed using high precision olfactometry and a go/no-go operant task. Additionally, ten rats were pre-treated with the D-2 receptor antagonist spiperone (0.62 mg/kg IP) and their performance monitored following quinpirole administration. Treatments were administered every third day in a counterbalanced order, with the quinpirole injections occurring 15 min before, and the spiperone injections 35 min before, the 260-trial test sessions. Quinpirole injection resulted in a dose-dependent decrease in odor detection performance, as measured by the percentage of correct trials and by the non-parametric signal detection sensitivity index SI. Prior treatment with spiperone eliminated these effects. Dose-related influences of quinpirole on (a) the average latency to initiate a detection response (i.e., the S + response latency), (b) the total session duration, and (c) the number of aborted trials were also eliminated or greatly attenuated by prior spiperone injection. These results suggest that D-2 receptors may be involved in the modulation of odor detection performance and related behaviors.

Topics: Acetates; Animals; Behavior, Animal; Conditioning, Operant; Dopamine Agents; Ergolines; Male; Quinpirole; Rats; Rats, Inbred Strains; Smell; Spiperone